Source: UNIVERSITY OF FLORIDA submitted to NRP
ENHANCING BIOACTIVE PHYTOCHEMICALS IN FRESH AND PROCESSED GUAVA (PSIDIUM GUAJAVA)
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
SPECIAL GRANT
Reporting Frequency
Annual
Accession No.
0200742
Grant No.
2004-34135-14632
Cumulative Award Amt.
(N/A)
Proposal No.
2004-05114
Multistate No.
(N/A)
Project Start Date
Sep 1, 2004
Project End Date
Aug 31, 2006
Grant Year
2004
Program Code
[AH]- (N/A)
Recipient Organization
UNIVERSITY OF FLORIDA
G022 MCCARTY HALL
GAINESVILLE,FL 32611
Performing Department
FOOD SCIENCE & HUMAN NUTRITION
Non Technical Summary
A. Health benefits of tropical fruits can be maximized by post-harvest treatments. B. Loss of fruit crops occur due to pest infestations. The influence on post harvest treatments to reduce pest infestation on beneficial phytochemicals is not known. The purpose of the experiments are to determine what occurs to beneficial phytonutrients during post harvest treatments.
Animal Health Component
60%
Research Effort Categories
Basic
30%
Applied
60%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
5031099100025%
7011099101075%
Knowledge Area
503 - Quality Maintenance in Storing and Marketing Food Products; 701 - Nutrient Composition of Food;

Subject Of Investigation
1099 - Tropical/subtropical fruit, general/other;

Field Of Science
1010 - Nutrition and metabolism; 1000 - Biochemistry and biophysics;
Goals / Objectives
Objective 1. To isolate and identify the predominant phytochemicals present in guava for assessment of their antioxidant and bioactive activities. Objective 2. To evaluate phytochemical content and bioactive properties of guava as affected by a thermal quarantine treatment and postharvest ripening. Objective 3. To evaluate pre-processing and processing variables that affects antioxidant and bioactive properties of guava juice and puree.
Project Methods
We are currently investigating the content and identity of guava polyphenolics and their distribution in the fruit. The postharvest treatments with hot water immersion will be repeated to insure the accuracy and repeatability of the trial, as this information has the potential to significantly impact guava fruit distribution world-wide. These studies will be followed by additional characterization by HPLC-MS, which we anticipate will be a rather lengthy procedure based on our previous work with other commodities. We will continue to take advantage of the detailed phytochemical fractionation procedures previously elucidated for guava to chemically and biochemically characterize the content and health promoting aspects of the fruit. We have completed our preliminary trials with processed guava juice, and will shortly begin phytochemical stability trials with the goal of commercial application. These characterizations will include investigating for the presence of cell wall bound polyphenolics, but only after identification of major intracellular compounds have been elucidated. Health benefits regarding the nature of the anticancer activity will also continue. The data gathered thus far is expected to result in presentations at two meetings (IFT and Experimental Biology) and at least two publications in major peer reviewed journals.

Progress 09/01/04 to 08/31/06

Outputs
Several tropical fruits including guava, mango, acai, and passionfruit, were shown to have a unique chemical makeup and that those chemical compounds provide health benefits related to their antioxidant and anti-cancer activities. Fractionation of these foods into related families of compounds do not provide the same degree of health benefits as the whole food. Guava induces caspase-3 and caspase-8, two enzymes in the apoptotic pathway resulting in death of cancer cells. A dose related increase in caspase activity was shown.

Impacts
Understanding the chemical composition of these and other tropical fruits is new information not previously known. The health benefits are driven by the chemical makeup and we can predict now what disease risk reductions may occur. Growers and marketers of tropical and sub-tropical fruits will be able to use this information to provide consumers with important nutritional knowledge.

Publications

  • Nantz, M.P., Rowe, C.A., Nieves, C.J., and Percival, S.S. 2006 Immunity and antioxidant capacity in humans is enhanced by consumption of a fruit and vegetable juice concentrate. J Nutr. 136: 2606-2610, 2006.
  • Percival, S.S., Talcott, S.T., Chin, S.T., Mallak, A.C., Lounds-Singleton, A. and Pettit-Moore, J. 2006 Inhibition of neoplastic transformation and cancer cell cycle arrest by mango (Mangifera indica L.). J Nutr. 136:1-5, 2006.
  • Del Pozo-Insfran, D., Percival S.S. and Talcott, S.T. 2006 Acai (Euterpe oleracea Mart.) polyphenolics in the glycoside and aglycone forms induce apoptosis of HL-60 leukemia cells. J. Ag. and Food Chem. Published on the Web 1/12/2006.
  • Talcott S.T., Moore, J., Lounds-Singleton, A., and Percival, S.S. 2005 Ripening associated phytochemical changes in mangos (mangifera indica) following thermal quarantine and low-temperature storage. Journal of Food Science 70(5)337-41, 2005. Published on Web 6/14/2005.
  • Rowe, C. A., Nantz, M. P., DeNiera, C., Green, K., Talcott, S. T., and Percival, S. S. 2004 Inhibition of neoplastic transformation of benzo[]pyrene-treated Balb/c 3T3 murine cells by a phytochemical extract of passionfruit juice. J. Med. Food. 7(4):402-7, 2004.


Progress 10/01/04 to 09/30/05

Outputs
All activity reported under FLA-FOS-04080-P.

Impacts
All activity reported under FLA-FOS-04080-P.

Publications

  • No publications reported this period