Progress 09/01/04 to 08/31/08
Outputs
OUTPUTS: This project used a variety of molecular genetic tools and computational approaches to determine the processes that led to the biological invasion of the olive fruit fly, or olive fly, Bactrocera oleae, in new olive growing areas in the US and elsewhere. This project (1) investigated the process through which detailed knowledge of historical and present day population parameters are assembled for invasive species and (2) used modern computational approaches to infer the spatial expansion dynamics of invading populations of agricultural pests. In addition, results from this study will assisted with efforts (1) to plan integrated strategies of pest management, (2) to establish import-export regulations and quarantine practices, and (3) to assess risks and benefits of foreseeable SIT (sterile insect techniques) programs to restrict the spread of invading populations. The results were disseminated through publications, symposia, and workshops. PARTICIPANTS: Collaborations: Nardi, F. University of Siena, Italy, molecular genetics of olive fly Hoelmer, K. USDA, Montpellier, France Bon, M-C, USDA Montpellier, France Navajas, M. INRA, Montpellier, France, studies of invasive species in Mediterranean regions. invasions Research Coordination Network, NSF-funded grant (PI Roderick) to collaborate with 35 scientists in 12 countries to study invasive insect species. TARGET AUDIENCES: College and university students Academic researchers Government and private researchers Managers K-12 students Public Political decision makers PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Olive fly is an emerging pest in the US and its study is a model for the analysis of population history and expansion through genetic and computational methods. We analyzed variation within and among populations of olive flies from microsatellite loci which we developed and mitochondrial DNA. Many of the collections were made as part of exploration for classical biological control agents. We have also examined genetic data from previous work on olive fly. Complete mitochondrial sequences have been completed for "population-sized" samples. We are continuing to develop demographic models to understand the historical demography of the olive fly invasions. Results from nuclear markers and genes for insecticide resistance suggest that (1) the history of olive fly parallels that of the domestication of olives, (2) the California populations have a recent origin in the Mediterranean region, and (3) that presence of alleles for insecticide resistance in some populations reflects both past use of insecticides and recent patterns of immigration. Results that have broader implications for biological invasions generally, are, (1) the populations introduced into the Americas represented only a small sample of the genetic variation of potential source populations and (2) invasive populations were "pre-adapted" to certain control methods based on previous history. Future work will include collaborations with USDA and California researchers to obtain genetic material from olive flies collected worldwide to conduct similar analyses.
Publications
- Navajas M, Roderick GK (2008) Molecular diagnosis. (ed. Capinera JL) Encyclopedia of Entomology, pp. 191-196. Springer, Dordrecht.
- Roderick GK, Navajas M (2008) The primacy of evolution in biological control. In: Proceedings of the XII International Symposium on Biological Control of Weeds (eds. Julien MH, Sforza R, Bon MC, Evans HC, Hatcher PE, Hinz HL, Rector BG), p. 411-177. CAB International, Wallingford, UK.
- Nardi F, Carapelli A, Vontas JG, Dallai R, Roderick GK, Frati F (2006) Geographical distribution and evolutionary history of organophosphate-resistant Ace alleles in the olive fly (Bactrocera oleae). Insect Biochemistry and Molecular Biology 36, 593-602.
- Clarke AR, Armstrong KF, Carmichael AE, Milne JR, Raghu S, Roderick GK, Yeates DK (2005) Invasive phytophagous pests arising through a recent tropical evolutionary radiation: The Bactrocera dorsalis complex of tropical fruit flies. Annual Review of Entomology 50, 293-319.
- Davies N, Roderick GK (2005) Dipteran sex chromosomes in evolutionary developmental biology. (eds. Wiegmann B, Yeates D) Evolutionary Biology of Flies, p. 196-213. New York: Columbia University Press.
- Hufbauer RA, Roderick GK (2005) Microevolution in biological control: mechanisms, patterns, and processes. Biological Control 35 (3), 181-193.
- Nardi F, Crapelli A, Dallai R, Roderick GK, Frati F (2005) Population structure and colonization history of the olive fly, Bactrocera oleae (Diptera, Tephritidae). Molecular Ecology 14: 2729-2738.
- Savolainen V, Cowan RS, Vogler AP, Roderick GK, Lane R (2005) Towards writing the encyclopaedia of life: an introduction to DNA barcoding. Philosophical Transactions of the Royal Society: Biological Sciences 360, 1805-1811.
- Roderick GK (2004) Tracing the origin of pests and natural enemies: genetic and statistical approaches. (eds. Ehler LE, Sforza R, Mateille T). Genetics, Evolution, and Biological Control, pp. 97-112. CAB International, Wallingford, UK.
- APEC (2006) Invasive Alien Species (IAS) Strategy Framework Implementation, SOM Steering Committee on Economic and Technical Cooperation (SCE), Asia-Pacific Economic Cooperation. Da Nang, Viet Nam, 2006/SOM3/SCE/009. Available on the web, http://aimp.apec.org/Documents/2006/SCE/SCE3/06_sce3_009.doc Policy statement resulting from APEC Workshop, Beijing, 2005.
- Zhang F-T, Roderick GK, eds. (2005) APEC Workshop on Invasive Alien Species, Proceedings. Ministry of Agriculture, Beijing, China.
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Progress 01/01/07 to 12/31/07
Outputs
OUTPUTS: The research uses molecular genetic tools and computational approaches to determine the processes that led to the biological invasion of the olive fruit fly, or olive fly, Bactrocera oleae, in new olive growing areas in the US and elsewhere. . Genetic markers include mitochondrial and nuclear DNA, including microsatellites and genes for insecticide resistance. Analysis includes include traditional FST-based approaches and as well as novel non-equilibrium molecular and computational approaches. This project will also serve as a model system for the study of biological invasions of agricultural insect pests. Two general outcomes can be generalized to other systems: (1) the process through which detailed knowledge of historical and present day population parameters are assembled for invasive species and (2) the use of modern computational approaches to infer the spatial expansion dynamics of invading populations of agricultural pests. In addition, results from this study will
assist with efforts (1) to plan integrated strategies of pest management, (2) to establish import-export regulations and quarantine practices, and (3) to assess risks and benefits of foreseeable SIT (sterile insect techniques) programs to restrict the spread of invading populations.
PARTICIPANTS: Francesco Nardi, Univ. Siena, Italy George Roderick, UC Berkeley CBGP, INRA, Montferrier-sur-Lez, nr Montpellier, France
TARGET AUDIENCES: Information is targeted at research scientists, managers, students at all levels, and the general public with an interest in growing olives for harvest or as ornamentals.
PROJECT MODIFICATIONS: One year no cost extension granted to continue sampling in California and Mediterranean regions
Impacts
To date, we have created an enriched microsatellite library prepared from olive fly DNA and have analyzed variation within and among populations. We are collaborating with USDA and California researchers to obtain genetic material from olive flies collected worldwide. Many of the previous collections were made as part of exploration for classical biological control agents. We have also examined genetic data from previous work on olive fly. We are continuing to develop demographic models to understand the historical demography of the olive fly invasions. Results from nuclear markers and genes for insecticide resistance suggest that (1) the history of olive fly parallels that of the domestication of olives, (2) the California populations have a recent origin in the Mediterranean region, and (3) that presence of alleles for insecticide resistance in some populations reflects both past use of insecticides and recent patterns of immigration. Results that have broader
implications for biological invasions generally, are, (1) the populations introduced into the Americas represented only a small sample of the genetic variation of potential source populations and (2) invasive populations were "pre-adapted" to certain control methods based on previous history. Olive fly is an emerging pest in the US and its study will be a model for the analysis of population history and expansion through genetic and computational methods.
Publications
- Gillespie RG, Claridge EM, Roderick GK (2007) Natural and human-mediated biodiversity dynamics in isolated island communities. Proceedings, 3rd Meeting in Ecology and Behaviour, Montpellier, France, March 2007
- Roderick GK, Navajas M (2007) Biological control meets evolutionary biology in the South of France. In XIIth International Symposium on Biological Control of Weeds, Abstracts USDA/CSIRO Montpellier, France, La Grande Motte, France. p. 51
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Progress 01/01/06 to 12/31/06
Outputs
To date, we have created an enriched microsatellite library prepared from olive fly DNA and have analyzed variation within and among populations. This library was created based on attempted enrichments from 7 microsatellite motifs: CA, ATG, AAT, CAG, TACA, CATC, and TAGA. We are collaborating with USDA and California researchers to obtain genetic material from olive flies collected worldwide. Many of the previous collections were made as part of exploration for classical biological control agents. We have also examined genetic data from previous work on olive fly. We are initiating the modeling effort to understand the historical demography of the olive fly invasions. Results from nuclear markers and genes for insecticide resistance suggest that (1) the history of olive fly parallels that of the domestication of olives, (2) the California populations have a recent origin in the Mediterranean region, and (3) that presence of alleles for insecticide resistance in some
populations reflects both past use of insecticides and recent patterns of immigration.
Impacts
Olive fly is an emerging pest in the US and its study will be a model for the analysis of population history and expansion through genetic and computational methods.
Publications
- Chen YH, Opp SB, Berlocher SH, Roderick GK (2006) Are bottlenecks associated with colonization? Genetic diversity and diapause variation of native and introduced Rhagoletis completa populations. Oecologia 149, 656-667.
- Nardi F, Carapelli A, Vontas JG, Dallai R, Roderick GK, Frati F (2006) Geographical distribution and evolutionary history of organophosphate-resistant Ace alleles in the olive fly (Bactrocera oleae). Insect Biochemistry and Molecular Biology 36, 593-602.
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Progress 01/01/05 to 12/31/05
Outputs
To date, we have created an enriched microsatellite library prepared from olive fly DNA and are analyzing variation within and among populations. This library was created based on attempted enrichments from 7 microsatellite motifs: CA, ATG, AAT, CAG, TACA, CATC, and TAGA. We continue to assess this library and to use the markers on material collected worldwide. We have also examined genetic data from previous work on olive fly. We are initiating the modeling effort to understand the historical demography of the olive fly invasions.
Impacts
Olive fly is an emerging pest in the US and its study will be a model for the analysis of population history and expansion through genetic and computational methods.
Publications
- Savolainen, V., Cowan, R.S., Vogler, A.P., Roderick, G.K., Lane, R. 2005. Towards writing the encyclopaedia of life: an introduction to DNA barcoding. Philosophical Transactions of the Royal Society: Biological Sciences 360, 1805-1811.
- Clarke, A.R., Armstrong, K.F., Carmichael, A.E., Milne, J.R., Raghu, S., Roderick, G.K., Yeates, D.K. 2005. Invasive phytophagous pests arising through a recent tropical evolutionary radiation: The Bactrocera dorsalis complex of tropical fruit flies. Annual Review of Entomology 50: 293-319.
- Davies, N., Roderick, G.K. 2005. Dipteran sex chromosomes in evolutionary developmental biology. In: Wiegmann B, Yeates D, editors. Evolutionary Biology of Flies. New York: Columbia University Press. pp. 196-213.
- Hufbauer, R.A., Roderick, G.K. 2005 Microevolution in biological control: mechanisms, patterns, and processes. Biological Control 35: 227-239.
- Nardi, F., Crapelli, A., Dallai, R., Roderick, G.K., Frati, F. 2005. Population structure and colonization history of the olive fly, Bactrocera oleae (Diptera, Tephritidae). Molecular Ecology 14: 2729-2738.
- Savolainen, V., Cowan, R.S., Vogler, A.P., Roderick, G.K. 2005. DNA barcoding of life. Papers of a Theme Issue. In: Philosophical Transactions of the Royal Society: Biological Sciences, pp. 1803-1980. Philosophical Transactions of the Royal Society: Biological Sciences. 360 (1462).
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Progress 01/01/04 to 12/31/04
Outputs
This project will determine the processes that led to the biological invasion of the olive fruit fly, or olive fly, Bactrocera oleae, in new olive growing areas in the US and elsewhere. This project will also serve as a model system for the study of biological invasions of agricultural insect pests. Two general outcomes can be generalized to other systems: (1) the process through which detailed knowledge of historical and present day population parameters are assembled for invasive species and (2) the use of modern computational approaches to infer the spatial expansion dynamics of invading populations of agricultural pests. In addition, results from this study will assist with efforts (1) to plan integrated strategies of pest management, (2) to establish import-export regulations and quarantine practices, and (3) to assess risks and benefits of foreseeable SIT or GESIT programs to restrict the spread of invading populations. To date, we have created an enriched
microsatellite library prepared from olive fly DNA. This library was created based on attempted enrichments from 7 microsatellite motifs: CA, ATG, AAT, CAG, TACA, CATC, and TAGA. Work is now ongoing to assess this library and to use the markers on material collected world-wide. We have also examined genetic data from previous work on olive fly and this work is now in press.
Impacts
Olive fly is an emerging pest in the US and its study will be a model for the analysis of population history and expansion through genetic and computational methods.
Publications
- Armstrong, K.F. and Roderick, G.K. 2004. Molecular markers in identification: lessons from the Tephritidae Proceedings of the XXII International Congress of Entomology. Brisbane, Australia.
- Roderick, G.K. 2004. Tracing the origin of pests and natural enemies: genetic and statistical approaches. In: Genetics, Evolution, and Biological Control (eds. Ehler LE, Sforza R, Mateille T), pp. 97-112. CAB International, Wallingford, UK.
- Roderick, G.K. and Hufbauer, R.A. 2004. Genetic, molecular, & analytical tools for refining classical biological control. Proceedings of the XXII International Congress of Entomology. Brisbane, Australia.
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