Source: OKLAHOMA STATE UNIVERSITY submitted to NRP
VACUUM FOR POST HARVEST DISINFESTATION OF INSECTS FROM DURABLE AND FRESH COMMODITIES
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0200706
Grant No.
2004-51102-02194
Cumulative Award Amt.
(N/A)
Proposal No.
2004-04007
Multistate No.
(N/A)
Project Start Date
Aug 1, 2004
Project End Date
Jul 31, 2007
Grant Year
2004
Program Code
[112.C]- (N/A)
Recipient Organization
OKLAHOMA STATE UNIVERSITY
(N/A)
STILLWATER,OK 74078
Performing Department
ENTOMOLOGY & PLANT PATHOLOGY
Non Technical Summary
The bruchid, Callosobruchus maculatus, commonly known as the cowpea weevil, is an important field-to-store pest of cowpeas and other grain legumes. The level of infestation of cowpeas in the field can be low and thus difficult to detect, however these low populations can increase to damaging numbers in storage. The goal of this research is to investigate the effectiveness of low pressure simulating vacuum as an alternative to fumigation in the disinfestation cowpeas and other grain legumes in storage.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21615991130100%
Goals / Objectives
The purpose of this reasearch is on the application of low pressure, or vacuum, as an alternative to methyl bromide for insect disinfestation of postharves agricultural commodities. Vacuum reduces all the atmospheric gases from a system and imposes a low oxygen atmosphere. Commercial scale application of vacuum is proposed using flexible PVC chambers, referred to as "Cocoons", that are evacuated using an electric vacuum pump.
Project Methods
1. Validate the treatment parameters of pressure, exposure time and temperature required to control the cowpea weevil infesting commercial sized lots of cowpeas, a stored-product group that has not been studied so far. 2. Develop methods to utilize vacuum cocoons to treat fresh commodities withoug damage using a novel technology 3. Research the possibility of combining low doses of natural volatile toxicant ethyl formate with vacuum to enhance or improve mortality of certain postharvest pests, perhaps shortening treatment times. 4. Present our results to various user and industry groups through a series of extension and outreach programs and publish media on a regional and nationwide scale.

Progress 08/01/04 to 07/31/07

Outputs
Application of vacuum, or low pressure, to a sealed system reduces the amount of all atmospheric gases and creates a low-oxygen atmosphere that kills insects through suffocation while causing no harm to durable agricultural products. Laboratory studies found that vacuum treatment at 50 mm Hg could kill all life stages of major stored product insects, and that generally the egg and the pupal stages were the most tolerant to vacuum. A commercial scale demonstration of vacuum treatments was conducted at a dried bean plant in California. A 5-ton "Cocoon" attached to an electric vacuum pump was used to treat 100-lb sacks of dried beans at the company's warehouse. Ventilated jars of beans infested with different life stages of the cowpea weevil were placed in the treatment to monitor efficacy. A large PITs Tunnel system was also tested at the bean plant. The PITs tunnel is a flexible PVC chamber about the size and dimensions of a 20-foot shipping container and when empty it is held open by a metal frame. One-ton cloth tote bags of bean were loaded into the PITs tunnel using a forklift; a total of 10 totes were loaded. The PITs tunnel was sealed and evacuated with a pump to approximately 50 mm of Hg and held overnight. Additional laboratory work showed that the duration of an effective vacuum treatment could be reduced to four hours by the addition of low levels of the botanical insecticide ethyl formate. Educational programs about vacuum treatments were presented to groups of potential users at the national workshop on storage and handling of organic stored grains at Purdue University, West Lafayette, IN, at the national Pest Control Technology seminar held in Minneapolis, MN, and at the VIIth National Stored Product IPM Training Conference in Stillwater, OK.

Impacts
Vacuum treatments for high value commodities represent effective methods to control insect pests without use of any chemicals. Thus, vacuum represents an organically compliant method of pest control, and could be adopted by organic food companies or others who want to eliminate use of chemical insecticides. Pest control companies have used our information to help clients. Extension and training programs in the past year have shared information on vacuum treatment with several hundred potential users.

Publications

  • Mbata, G. N., M. Johnson, T. W. Phillips and M. Payton. 2005. Mortality of life stages of the cowpea weevil..exposed to low pressure at different temperatures. J. Econ. Entomol. 98: 1070-1075.
  • Thomas, E. and T. W. Phillips. 2004. Chapter 20. Fumigation. Pp. 1165-1261, In: Hedges, S. ed. Mallis Handbook of Pest Control, 9th edition. Mallis Publishing Co.
  • Phillips, T. W. 2006. The Science and Technology of Postharvest Insect Control: Challenges, Accomplishments and Future Directions. Chapter 19, pp. 211-222, In: J. Heaps, ed., Insect Management for Food Storage and Processing, 2nd Ed. Am. Assoc. Cereal Chem.


Progress 10/01/05 to 09/30/06

Outputs
Laboratory studies found that vacuum treatment at 50 mm of mercury could kill all life stages of the cowpea weevil, and that the pupal stage was the most tolerant to vacuum. A commercial scale demonstration of vacuum treatments was conducted at a dried bean plant in California. A 5-ton 'Cocoon' attached to an electric vacuum pump was used to treat 100-lb sacks of dried beans at the company's warehouse. Ventilated jars of beans infested with different life stages of the cowpea weevil were placed in the treatment to monitor efficacy. A large PITs Tunnel system was also tested at the bean plant. The PITs tunnel is a flexible PVC chamber about the size and dimensions of a 20-foot shipping container and when empty it is held open by a metal frame. One-ton cloth tote bags of bean were loaded into the PITs tunnel using a forklift; a total of 10 totes were loaded. The PITs tunnel was sealed and evacuated with a pump to approximately 50 mm of Hg and held overnight. Educational programs about vacuum treatments were presented to groups of extension educators and potential users at the VIIth National Integrated Pest Management Symposium in St. Louis, MO, 5 April 2006. Extension publications are being prepared on vacuum technology and other controlled or modified atmosphere methods for insect control.

Impacts
Vacuum treatments for stored products represent effective methods to control pests in bagged commodities without use of any chemicals. Thus, vacuum represents and organically compliant method of pest control, and could be adopted by organic food companies or others who want to eliminate use of chemical insecticides.

Publications

  • Phillips, T. W. 2006. The Science and Technology of Postharvest Insect Control: Chapter 19, pp. 211-222, In: J. Heaps, ed., Insect Management for Food Storage and Processing, 2nd Ed. Am. Assoc. Cereal Chem.


Progress 10/01/04 to 09/30/05

Outputs
Laboratory studies found that vacuum treatment at 50 mm Hg could kill all life stages of the cowpea weevil, and that the pupal stage was the most tolerant to vacuum. A commercial scale demonstration of vacuum treatments was conducted at a dried bean plant in California. A 5-ton 'Cocoon' attached to an electric vacuum pump was used to treat 100-lb sacks of dried beans at the company's warehouse. Ventilated jars of beans infested with different life stages of the cowpea weevil were placed in the treatment to monitor efficacy. A large PITs Tunnel system was also tested at the bean plant. The PITs tunnel is a flexible PVC chamber about the size and dimensions of a 20-foot shipping container and when empty it is held open by a metal frame. One-ton cloth tote bags of beans were loaded into the PITs tunnel using a forklift; a total of 10 totes were loaded. The PITs tunnel was sealed and evacuated with a pump to approximately 50 mm of Hg and held overnight. Educational programs about vacuum treatments were presented to groups of potential users at the national workshop on storage and handling of organic stored grains at Purdue University, West Lafayette, IN, and also at the national Pest Control Technology seminar held in Minneapolis, MN.

Impacts
Vacuum treatments for stored products represent effective methods to control pests in bagged commodities without use of any chemicals. Thus, vacuum represents and organically compliant method of pest control, and could be adopted by organic food companies or others who want to eliminate use of chemical insecticides.

Publications

  • Mbata, G. N., M. Johnson, T. W. Phillips and M. Payton. 2005. Mortality of life stages of the cowpea weevil, Callosobruchus maculaturs (F.) (Coleoptera: Curculionidae), exposed to low pressure at different temperatures. J. Econ. Entomol. 98: 1070-1075.


Progress 10/01/03 to 09/30/04

Outputs
Ethyl formate is a naturally occurring volatile in some grains, fruits and vegetables that is insecticidal; it is exempted from a requirement of a tolerance by EPA and it is generally regarded as safe (GRAS) as a food additive. Laboratory studies found that the most tolerant life stages of key stored grain pests could be killed with exposure to ethyl formate, and that mortality could be increased by increase in temperature and by a combination treatment with a partial vacuum. Ethyl formate formulated with carbon dioxide will be tested in the future under commercial conditions. Ethyl formate may offer a low risk, natural product alternative to traditional chemical insecticides for commodity disinfestation. An investigation into the effect of low pressure alone on apples infested with apple maggots, Rhagoletis pomonella, revealed that it may also be useful for disinfesting perishable commodities. Infested apples were treated at 25 and 50 mm Hg pressures at 25 and 30 degrees C for exposure period ranging from 2 to 120 hours. Mortality was determined by comparing with the controls at ambient pressure. Eggs were the most tolerant stage followed by larvae. Exposure time for 99% mortality decreased with increase in temperature and pressure. However, at 30 degrees C the quality of apples in terms of appearance and taste may be affected.

Impacts
These results suggest a new use for ethyl formate as an insecticide when combined with vacuum, and can lead to a new product for pest control.

Publications

  • Mbata, G. N., T. W. Phillips and M. Payton. 2004. Mortality of eggs of stored-product insects held under vacuum: effects of pressure, temperature and exposure time. J. Econ. Entomol. 97: 695-702.