Progress 10/01/04 to 09/30/09
Outputs
OUTPUTS: The Biotechnology Core Facility at Kansas State University continues to provide a number of unique services to members of the Kansas Agricultural Experiment Station. These services include: the centralized ordering of oligopeptides, protein/peptide sequencing, peptide mapping, mass spectral analysis of proteins/peptides and other small molecules, detection of post-translationally modified peptide sequences, and the preparation of peptide based substrates for proteolytic enzymes and measuring ligand-receptor interactions using plasmon resonance. Ruth Welti and John Tomich spent the funds from a 45-8 "Major Research Instrumentation" grant to the National Science Foundation. We have assembled a state of the art functional genomics center at K-State, which is still being supported in part by a KSU Targeted Excellence Award. The Biotech Core Lab proteomics facility allows for the identification of proteins/peptides by nanoflow HPLC or 2-D gels by high resolution MALDI TOF/TOF and ESI-Ion Trap mass spectrometry. We applied for funds to acquire a new MS instrument with enhanced capabilities over our current machines. The funds were part of an NIH COBRE grant submitted by the university. The grant received a good yet not funded score. A revised application will be submitted in spring 2010. It had a number of repairs that needed to be made to both the MALDI TOF/TOF and ESI-Ion Trap instruments totaling more than $12,000. These repairs were carried out by the lab staff thereby saving more $8,000 that would have been spent on bringing in a company service engineer. This past year the long-time Associate Director Dr. Takeo Iwamoto stepped down to become a tenured professor at Jikei University in Tokyo Japan. Dr. Yasuaki Hiromasa was promoted to the position in September 2009. His background is in mass spectral analyses. PARTICIPANTS: We had 93 AES approved projects make use of the oligo-nucleotide service, 40 projects used the mass spectroscopy service, 12 projects used the peptide/protein synthesis/ separation service and 10 other projects used other services provided by the core lab. TARGET AUDIENCES: We primarily serve KAES research faculty from K-State, and in particular the entomology and plant science communities. We also accepted samples from other K-State research faculty as well as from other universities and companies, when time permitted. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
We continued serving the research needs of KAES supported faculty, research fellows and students. By centralizing expensive and specialized instrumentation under the operation and maintenance of a single center, a large number of molecular biological and protein researchers are aided more economically. During the previous five year award the facility has assisted more than 92 different laboratories supported by KAES.
Publications
- Marshall, J. L, Huestis, D.L., Hiromasa, Y., Oppert, C., Marshall, S.A., Tomich, J.M. and Oppert, B. (2009) Identification, RNAi knockdown, and functional analysis of an ejaculate protein that mediates a postmating, prezygotic phenotype in a cricket. PLoS ONE 4(10): e7537 (10 pgs). PMID: 19851502
- Martin, J., Devlin, S., Malreddy, P., Iwamoto, T., Freeman, L., Davidson, H., Tomich, J.M. and Schultz, B.D., (2009) NC-1059, A Channel-Forming Peptide Modulates Drug Delivery Across In Vitro Corneal Epithelium. Invest. Ophthal. Vis Sci. 50(7):3337-3345. PMID: 19234338
- Thomas, V. C., Hiromasa, Y., Harms, N., Thurlow, L., Tomich, J., Hancock, L. (2009) A fratricidal mechanism is responsible for eDNA release and contributes to biofilm development of Enterococcus faecalis, Molecular Microbiology 72(4):1022-1036. PMID: 19400795
- Morris, K., Lorenzen, M., Hiromasa, Y., Tomich, J.M. Oppert, C., Elpidina, E.N., Vinokurov, K., Jurat-Fuentes, J.L., Fabrick, J., Oppert, B.S. (2009) The Tribolium castaneum larval gut transcriptome and proteome: A resource for the study of the coleopteran gut. J Proteome Res. 8(8):3889-3898. PMID: 19545177
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Progress 01/01/08 to 12/31/08
Outputs
OUTPUTS: The Biotechnology Core Facility at Kansas State University continues to provide a number of unique services to members of the Kansas Agricultural Experiment Station. These services include: the synthesis of oligopeptides, protein/peptide sequencing, peptide mapping, mass spectral analysis of proteins and peptides, detection of post-translationally modified peptide sequences, and the preparation of peptide based substrates for proteolytic enzymes and measuring ligand-receptor interactions using plasmon resonance. Ruth Welti and John Tomich spent the funds from a 45-8 "Major Research Instrumentation" grant to the National Science Foundation. We have assembled a state of the art functional genomics center at K-State, which is still being supported in part by a KSU Targeted Excellence Award. The Biotech Core Lab proteomics facility allows for the identification of proteins/peptides by nanoflow HPLC or 2-D gels by high resolution MALDI TOF/TOF and ESI-Ion Trap mass spectrometry. In the past year we have upgraded the software on several of the proteomics instruments such that we can now perform DIGE analyses which identify differences in protein expression using 2D-gels, containing mixtures labeled with different fluorescent dyes. We have been invited to submit a new Targeted Excellence proposal for the purchase of a new mass spectrometer that will greatly increase our ability to serve the insect researchers on campus. PARTICIPANTS: We had 40 AES members use our oligonucleotide service, 30 faculty members used the Mass spectroscopy service, 15 faculty use the peptide/protein synthesis/ separation service and 12 other faculty use other services provided by the core lab. TARGET AUDIENCES: We primarily serve KAES research faculty from K-State, and in particular the entomology community. We also accept samples from other K-State research faculty as well as from other universities and companies, time permitting. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
We plan to continue serving the research needs of KAES supported faculty, research fellows and students. By centralizing expensive and specialized instrumentation under the operation and maintenance of a single center, a large number of molecular biological and protein researchers are aided more economically. During the previous five year award the facility has assisted more than 86 different laboratories supported by KAES.
Publications
- Frederik W. van Ginkel, Takeo Iwamoto, Bruce D. Schultz and John M. Tomich (2008) Peptide-Specific Immunity to Self-derived, Anion Channel-Forming Peptides in the Respiratory Tract. Clinical and Vaccine Immunology. 15(2):260-266.
- Xiaoqun Mo, Yasuaki Hiromasa, Ahlam Al-Rawi, Matt Warner, Takeo Iwamoto, Talat Rahman, Xiuzhi Sun and John M. Tomich. (2008) Design of 11-Residue Peptides with Unusual Biophysical Properties: Induced Secondary Structure in the Absence of Water. Biophysical J. 94:1807-1817.
- Yuntao Zhang, Takeo Iwamoto, John M. Tomich, Yutaka Kariya, Kiyoshi Suzuki, Abigain H. Conrad, Gary W. Conrad (2008) MALDI-TOF/TOF-MS for the Analysis of Keratan Sulfate Oligosaccharides J. Mass Spectrom. 43(6):765-772
- Suma Somasekharan, Robert Brandt, Takeo Iwamoto, John M. Tomich, and Bruce D. Schultz (2008) Modulation of Epithelial Barrier Function by a Synthetic Peptide. J. Membr. Biol. 222: 17-30.
- Gwi-Moon Seo, Chuanmin Cheng, John M. Tomich and Roman R. Ganta1 (2008) Total, membrane, and immunogenic proteomes of macrophage- and tick cell-derived Ehrlichia chaffeensis evaluated by LC-MS/MS and MALDI-TOF methods. Infection and Immunity. 76: 4823-4832.
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Progress 01/01/07 to 12/31/07
Outputs
OUTPUTS: The Biotechnology Core Facility at Kansas State University continues to provide a number of unique services to members of the Kansas Agricultural Experiment Station. These services include: the synthesis of oligopeptides, protein/peptide sequencing, peptide mapping, mass spectral analysis of proteins and peptides, detection of post-translationally modified peptide sequences, and the preparation of peptide based substrates for proteolytic enzymes and measuring ligand-receptor interactions using plasmon resonance. Ruth Welti and John Tomich spent the funds from a 45-8 "Major Research Instrumentation" grant to the National Science Foundation. We have assembled a state of the art functional genomics center at K-State, which is supported in part by a KSU Targeted Excellence Award. The Biotech Core Lab proteomics facility allows for the identification of proteins/peptides by nanoflow HPLC or 2-D gels by high resolution MALDI TOF/TOF and ESI-Ion Trap mass spectrometry. In the past
year we have upgraded the software on several of the proteomics instruments such that we can now perform DIGE analyses which identify differences in protein expression using 2D-gels, containing mixtures labeled with different fluorescent dyes.
PARTICIPANTS: We had 39 AES members use our oligonucleotide service, 33 faculty use the Mass spectroscopy service, 10 faculty use the peptide synthesis service and 13 other faculty use other services provided by the core lab.
TARGET AUDIENCES: We primarily serve KAES research faculty from K-State, but do accept samples from other K-State research faculty as well as from other universities and companies, time permitting.
Impacts
We plan to continue serving the research needs of KAES supported faculty, research fellows and students. By centralizing expensive and specialized instrumentation under the operation and maintenance of a single center, a large number of molecular biological and protein researchers are aided more economically. During the previous five year award the facility has assisted more than 80 different laboratories supported by KAES.
Publications
- No publications reported this period
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Progress 01/01/06 to 12/31/06
Outputs
The Biotechnology Core Facility at Kansas State University provides a number of unique services to members of the Kansas Agricultural Experiment Station. These services include: the synthesis of oligopeptides, protein/peptide sequencing, peptide mapping, mass spectral analysis of proteins and peptides, detection of post-translationally modified peptide sequences, and the preparation of peptide based substrates for proteolytic enzymes and measuring ligand-receptor interactions using plasmon resonance. Ruth Welti and John Tomich spent the funds from a 45-8 "Major Research Instrumentation" grant to the National Science Foundation. We have assembled a state of the art functional genomics center at K-State. The Biotech Core Lab created a proteomics facility to allow for the identification of proteins/peptides by nanoflow HPLC or 2-D gels by high resolution MALDI TOF/TOF and ESI-Ion Trap mass spectrometry.
Impacts
We plan to continue serving the research needs of KAES supported faculty, research fellows and students. By centralizing expensive and specialized instrumentation under the operation and maintenance of a single center, a large number of molecular biological and protein researchers are aided more economically. During the previous five year award the facility has assisted more than 80 different laboratories supported by KAES.
Publications
- No publications reported this period
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Progress 01/01/05 to 12/31/05
Outputs
The Biotechnology Core Facility at Kansas State University provides a number of unique services to members of the Kansas Agricultural Experiment Station. These services include: the synthesis of oligopeptides, protein/peptide sequencing, peptide mapping, mass spectral analysis of proteins and peptides, detection of post-translationally modified peptide sequences, and the preparation of peptide based substrates for proteolytic enzymes and measuring ligand-receptor interactions using plasmon resonance. Ruth Welti and John Tomich submitted and were awarded a 45-8 Major Research Instrumentation grant to the National Science Foundation. We have acquired funds ($1.5 Million) to assemble a state of the art functional genomics center at K-State. The Protein Lab will create a proteomics facility to allow for the identification of proteins by nanoflow HPLC or 2-D gels by high resolution TOF/TOF mass spectrometry.
Impacts
We plan to continue serving the research needs of KAES supported faculty, research fellows and students. By centralizing expensive and specialized instrumentation under the operation and maintenance of a single center, a large number of molecular biological and protein researchers are aided more economically. During the previous five year award the facility has assisted more than 80 different laboratories supported by KAES.
Publications
- No publications reported this period
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Progress 01/01/04 to 12/31/04
Outputs
The Biotechnology Core Facility at Kansas State University provides a number of unique services to members of the Kansas Agricultural Experiment Station. These services include: the synthesis of oligopeptides, protein/peptide sequencing, peptide mapping, mass spectral analysis of proteins and peptides, detection of post-translationally modified peptide sequences, and the preparation of peptide based substrates for proteolytic enzymes and measuring ligand-receptor interactions using plasmon resonance. In 2004, 46 Agriculture Experiment Station affiliated laboratories in three separate colleges used one or more core lab services. During 2004 the Core Lab operation submitted its 5-Year Action Plan, which was approved and began on October 1, 2004. The lab also submitted an instrument proposal to the National Eye Institute at NIH for the purchase of a TOF/TOF mass spectrometer that would be used for proteomic studies. Unfortunately we did not receive a fundable score due
in large part, to a lack of institutional support from K-State. In addition the Biotech Core Lab joined Dr. Susan Sun's soybean adhesives project which was funded by a targeted excellence award that provides $50,000/yr for technical and material support. Together we have been preparing synthetic peptides with adhesive properties. At this point we have identified a highly adhesive peptide sequence that relies only on hydrophobic interactions for its strength. New versions of the peptide that include residues capable of cross-linking are being prepared that will further enhance the adhesive properties. A manuscript and a provisional patent application have been submitted.
Impacts
We serve the research needs of KAES supported faculty, research fellows and students. By centralizing expensive and specialized instrumentation under the operation and maintenance of a single center, a large number of molecular biological and protein researchers are aided more economically. Over the past nine years the facility has assisted more than 80 different laboratories supported by KAES.
Publications
- No publications reported this period
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