Progress 01/01/06 to 12/31/06
Outputs
Course contents were revised and several new multimedia modules were developed for teaching the core curriculum courses of Immunology (MBIO413), Virology & Immunology Laboratory (MBIO 414) as well as for the existing elective course Advanced Pathogenic Microbiology (MBIO 512). The Course Outline and syllabus for the Immunology (MBIO413) course is currently available on the Blackboard, a web-based software system currently being used by Tuskegee University. Guest lectures were provided by Dr. Peter Holt, USDA/ARS, Russell Research Center, Athens, GA (Avian Immunology) and Dr. Ron Schultz, Professor & Head, Department of Pathobiology, University of Wisconsin (Production & Use of Veterinary vaccines) and Dr. Irene Wesley (USDA National Animal Disease Center, Ames, IA). We sent two junior veterinary students (Ms. Melanie Muldrow and Ms. Tia Harris) for international research internship training at the Faculty of Veterinary Science in Nairobi, Kenya. One graduate student
received training at the University of Wisconsin Veterinary Diagnostic Laboratory on the use of real-time PCR assay. Two graduate students received partial financial support and supplies for their research and one student Ms. Alystia Walker participated in the Summer Internship program (8 weeks) at the USDA Southeast Poultry Research Laboratory. Three graduate students have completed the M.S degree program in veterinary science (Mr. Yezeed Abdelmageed Ms. Maria Bai-Sheka and Ms. Alystia Walker) and used real-time PCR assays and microarray protocols for their research. Grant funds allowed us to train the graduate students in the molecular protocols. Students used these protocols with reagents and chemicals purchased from these grant funds. Two more students are extensively using the real-time PCR machine purchased with the grant funds. They are receiving partial financial support from the grant funds. The project received no-cost extension for 12 months. This was mainly to allow one
graduate student (Mr. Rufus Clinkscales) to complete his research and submit his thesis. This student was with another faculty member in the department who passed away suddenly before the student could initiate the assigned research project.
PRODUCTS: Three graduate students that were provided partial support graduated with a master's degree in veterinary science. Two students availed international internship opportunity at the faculty of Veterinary Medicine, University of Nairobi, Kenya.Guest Lectures were useful in updating syllabi. Syllabi for few core curriculum courses is updated and made available on BLACKBOARD, a web-based course management system.
OUTCOMES: A few courses taught in the Department of Pathobiology are updated and reflect new developments in the host immune responses, development and use of vaccines, food safety, etc. Three minority graduate students have graduated. One student has enrolled in an advanced degree program and two were successful in getting jobs of their choice. Three graduate students are continuing in the department and are using molecular assays already developed with grant funds.
DISSEMINATION ACTIVITIES: The two veterinary students that availed international internship have presented their experiences in the Student Chapter of the American Veterinary Medical Association meeting. They will present it in the Tuskegee University's Annual Veterinary Medical Symposium. One papaer was presented by the graduate student at the Annual Meeting of the American Society of Microbiology.
FUTURE INITIATIVES: Course Content and syllabus will be constantly updated for the identified courses and we indent to initiate new courses (Ex., Foreign Animal Diseases) as first batch of PhD students have enrolled in the Fall semester of 2006. Guest lectures will be invited to provide some lectures to the new PhD students. Students will also be sent to the identified USDA laboratory for training in research protocols for which facilities are not currently available at Tuskegee University, including infectious disease research in poultry.
Impacts
Graduate students at Tuskegee will be able to use state-of-the-art molecular assays in their research and in developing rapid methods for the detection of foodborne pathogens. These methods will be useful for the food industry. They are also involved in research to further understand the molecular mechanisms of involved in immunosuppression in animals due to management stressors such as induced molting or due to environmental toxicants such as mercury. Courses such as Immunology and Virology & Immunology Laboratory are being taught with the use of multimedia audio-visuals and problem-solving methodology. The new multiple-choice questions added to the question bank are boosting the confidence of veterinary students in taking the board examinations. Infrastructure needed to educate graduate students has vastly improved. Rapid methods developed for the detection of foodborne pathogens will be useful for the industry in assuring safety of the foods.
Publications
- Hashim, N.S.M,, S. Nadeem, K.K. Srivastava and P.G. Reddy. 2006. Rapid detection of Listeria monocytogenes by immunomagnetic separation and real-time PCR. Paper presented at the Annual Meeting of the American Society of Microbiology. Orlando, FL, May 21-25, 2006.
- Nadeem, S., K.K. Srivastava, , Hashim, N.S and P.G. Reddy. 2007. Rapid detection of Listeria monocytogenes by immunomagnetic separation and real-time PCR. Paper submitted to Journal of Food Safety.
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Progress 09/15/03 to 09/14/06
Outputs
Course contents were revised and several new multimedia modules were developed for teaching the newly started core curriculum courses of Immunology (MBIO413), Virology & Immunology Laboratory (MBIO 414) as well as for the existing elective course Advanced Pathogenic Microbiology (MBIo512) courses. The Course Outline and syllabus for the Immunology (MBIO413) course is currently available on the Blackboard 6, a web-based software system currently being used by Tuskegee University. Guest lectures were provided by: Dr. Peter Holt, USDA/ARS, Russell Research Center, Athens, GA (Avian Immunology), Dr. Irene Wesley, USDA/ARS NADC Ames, IA (Food Safety) Dr. Ron Schultz, Professor & Head, Department of Pathobiology, University of Wisconsin (Production & Use of Veterinary vaccines). A total of five veterinary students were given the opportunity for international research internship training at the Faculty of Veterinary Science at Sokoine Agricultural University, Morogor, Tanzania and University of Nairobi, Kenya. 1. Ms. Leonara Dickson (Summer 2004) 2. Ms. Shadawn salmond (Summer 2004) 3. Ms. Melanie Muldrow (Summer 2006) 4. Ms. Tia Harris (Summer 2006) 5. Ms. Lavonne Gaffin (Summer 2007) Two graduate students (Mr. Sadig Nadeem and Ms. Maria Bai-Sheka) received training at the University of Wisconsin Veterinary Diagnostic Laboratory on the use of real-time PCR assay. The following graduate students received partial financial support (stipends and/or tuition): 1. Ms. Nora Hasheem 2. Ms. Maria Bai-Sheka 3. Mr. Sadig Nadeem 4. Mr. Yezeed Abdelmageed 5. Mr. Rufus Clinkscales 6. Ms. Ensaaf Taha 7. Ms. Kaylyn Dillard Among the students shown above, the first four have graduated. Ms. Kaylyn Dillard went on to the DVM program but returned to the graduate program after one semester. She is curretly taking three courses and will start her research in the Summer of 2008. Mr. Rufus Clinkscales and Ms. Ensaaf Taha are likely to graduate in May 2008. One graduate student (Ms. Alystia Walker) participated in the Summer Internship program (8 weeks) at the USDA Southeast Poultry Research Laboratory. Four graduate students have completed the M.S degree program in veterinary science and used real-time PCR assays and microarray protocols for their research. PRODUCTS: Four graduate students have successfully completed their Master's degree programs and almost all of them used molecular techniques including real-time PCR. Syllabi for the identified courses have been updated, multimedia modules have been developed and the course outlines, lecture notes are now available for the students on the BLACKBOARD, a Web-based course management software. The Smart Cycler purchased with grant funds is being extensively used by graduate students and faculty in their research projects. OUTCOMES: Grant funds allowed us to train the graduate students in the molecular protocols. Students used these protocols with reagents and chemicals purchased from these grant funds. Two more students are extensively using the real-time PCR machine purchased with the grant funds. They are receiving partial financial support from the grant funds. Two full-length manuscripts are being prepared for publication in scientific journals. Course Content and syllabus will be constantly updated for the identified courses and we indent to initiate new courses (Ex., Foreign Animal Diseases) for the benefit of students in the new Integrative Biosciences Ph.D program. We will continue to invite Guest lectures to provide some lectures to the new PhD students.. Graduate students are able to use state-of-the-art molecular assays in their research and are involved in developing rapid methods for the detection of foodborne pathogens. These methods will be useful for the food industry. They are also involved in research to further understand the molecular mechanisms of involved in immunosuppression in animals due management stressors such as induced molting or due to environmental toxicants such as mercury. Courses such as Immunology and Virology & Immunology Laboratory are being taught with the use of multimedia audio-visuals and problem-solving methodology. The new multiple-choice questions added to the question bank are boosting the confidence of veterinary students in taking the board examinations. Infrastructure needed to educate graduate students has vastly improved. Rapid methods developed for the detection of foodborne pathogens will be useful for the industry in assuring safety of the foods. DISSEMINATION ACTIVITIES: Students returning from International research internship either at Sokoine Agricultural University, Tanzania or University of nairobi, Kenya presented their experiences at the Student Chapter of the American Veterinary Medical Association meetings as well as the Annual Veterinary Medical Symposium at Tuskegee University. Two students have also presented their research at the Annual Biomedical Research Symposium in the School of Veterinary Medicine at Tuskegee University as well as at the American Society of Microbiology Annual Meetings. Faculty members have also presented papers at scientific and international meetings. FUTURE INITIATIVES: 1. Continue to acquire resources, especially the equipment to teach molecular methods to graduate students pursuing research in Animal Health and Food Safety. 2. Apply for National Needs Fellowship funding to support master's and Ph.D students. 3. Continue to seek extramural funding for acquiring teaching resources and upgrading faculty skills for excellence in teaching. 4. Continue to seek funding for providing national and international externship programs for veterinary and graduate students. 5. Continue to seek funding for providing travel support for graduate students to present research papers at the National scientific Meetings such as the Annual Meeting of the American Society of Microbiology.
Impacts
Graduate students at Tuskegee will be able to use state-of-the-art molecular assays in their research and in developing rapid methods for the detection of foodborne pathogens. These methods will be useful for the food industry. They are also involved in research to further understand the molecular mechanisms of involved in immunosuppression in animals due to management stressors such as induced molting or due to environmental toxicants such as mercury. Courses such as Immunology and Virology & Immunology Laboratory are being taught with the use of multimedia audio-visuals and problem-solving methodology. The new multiple-choice questions added to the question bank are boosting the confidence of veterinary students in taking the board examinations. Infrastructure needed to educate graduate students has vastly improved. Rapid methods developed for the detection of foodborne pathogens will be useful for the industry in assuring safety of the foods.
Publications
- 1. Nadeem, S. 2005. Rapid detection of Listeria monocytogenes using immunomagnetic separation and real-time PCR assay. M.S. Thesis submitted to Tuskegee University, August 2005.
- 2. Hashim, N.S. 2004. Serological Survey for West Nile virus (WNV) infection in horses and goats. M.S. Thesis submitted to Tuskegee University, August 2004.
- 3. Walker, A., M. Corley, and P.G. Reddy. 2005. The effects of forced molting on the immune response of spent layers, M.S. Thesis submitted to Tuskegee University, May 2006.
- 4. Mohamed, A. 2007. Prevalence of Campylobacter jejuni in retail chicken in Macon and Lee counties of Alabama, May 2007.
- 5. Reddy, P.G. Surveliinace and diagnosis of West Nile virus infections. Key Note paper submitted for presentation at the Indian Society of Veterinary Medicine, Bangalore, India, February 22-24, 2006.
- 6. Bai-Sheka, Maria. 2006. Detection of virulence genes of Escherichia coli isolates from bovine calves clinical cases: Comparison of the shiga toxin I (STX I) sequences with those of pathogenic E. Coli from human cases. Graduated in May 2006.
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Progress 01/01/05 to 12/31/05
Outputs
Course contents were revised and new multimedia modules were developed for teaching the newly started core curriculum courses of Immunology (MBIO413), Virology & Immunology Laboratory (MBIO414) as well as for the existing elective course Advanced Pathogenic Microbiology (MBIO512) courses. Graduate students are receiving training needed to undertake food safety research at the molecular level. The course outline and syllabus for the Immunology (MBIO413) course is now available on the Blackboard 6, a web-based software system being used at Tuskegee University. Guest lectures were provided by Dr. Peter Holt, USDA/ARS, Russell Research Center, Athens, GA (Avian Immunology) and Dr. Ron Schultz, Professor & Head, Department of Pathobiology, University of Wisconsin (Production & Use of Veterinary vaccines). We are in the process of selecting two veterinary students for international research internship training at the Faculty of Veterinary Science in Nairobi, Kenya. One
graduate student received training at the University of Wisconsin Veterinary Diagnostic Laboratory on the use of real-time PCR assay. Four graduate students received partial financial support (stipends and/or tuition) and two of them have completed the M.S degree program in veterinary science. Two students that have already participated in the international research internship at the Faculty of veterinary Science in Tanzania presented seminars on their work at the student chapter of the American Veterinary Medical Association meetings in the school and also at the Annual Veterinary Medical Symposium.
PRODUCTS: Two graduate students have successfully completed the Master's degree program in Veterinary Science and have extensively used molecular assays including real-time PCR and microarray protocols. Reagents and chemicals used in these assays were purchased with the grant funds. With the input from visitng professors, we have updated course content and syllabi for the identified courses for the veterinary and graduate students. Two other graduate students are showing rapid progress in their thesis research work and are expected to graduate in May 2006. Two papers will be presented at the scientific meetings at the national level and one papaer at an international meeting.
OUTCOMES: Grant funds are allowing us to build the much needed infrastructure to train graduate students in the molecular protocols needed for research in food safety and food animal health. Students were able to use these protocols with reagents and chemicals purchased from the grant funds. Two more students are extensively using the real-time PCR machine purchased with the grant funds. The students graduating with the M.S. degree will be competitive both in the job market and admission to PhD programs. Improved methodologies for teaching the identified courses for Veterinary students is increasing their satisfaction as evidenced in their evaluations of the instructor and the course ontent. The grant is helping us to strengthen the collaboration with the identified USDA laboratories and is proving to be mutually beneficial.
DISSEMINATION ACTIVITIES: Two students will be presenting their research work at national meetings including the American Society of Microbiology and at the Annual Biomedical Research Symposium at Tuskgee University. Two full-length manuscripts are being prepared for publication in scientific journals
FUTURE INITIATIVES: Course Content and syllabus will be constantly updated for the identified courses and we intend to initiate new courses (Ex., Foreign Animal Diseases) as first batch of PhD students are expected to enroll in the Fall semester. We will seek funds from other sources to provide international experiential learning opportunities for veterinary students as we have realized the importance of this program in the context of increasing globalization of animal trade. The new PhD program at Tuskegee will be stabilized with input from Guest Lectures. Students will be sent to the USDA laboratories for training in research protocols for which facilities are not currently available at Tuskegee University, including infectious disease research in poultry.
Impacts
Graduate students are able to use state-of-the-art molecular assays in their research and are involved in developing rapid methods for the detection of foodborne pathogens. These methods are likely to be useful for the food industry. They are also involved in research to further understand the molecular mechanisms involved in immunosuppression in animals due to management stressors such as induced molting or due to environmental toxicants such as mercury. Courses such as Immunology and Virology & Immunology Laboratory are being taught with the use of multimedia audio-visuals and problem-solving methodology. The new multiple-choice questions added to the question bank are boosting the confidence of veterinary students in taking the board examinations. Infrastructure needed to educate graduate students at Tuskegee University in the areas of food safety and food animal health has vastly improved.
Publications
- Nadeem, S. 2005. Rapid detection of Listeria monocytogenes using immunomagnetic separation and real-time PCR assay. M.S. Thesis submitted to Tuskegee University, August 2005.
- Hashim, N., K.K. Srivastava, S. Nadeem, and P.G. Reddy. 2005. Rapid detection of Listeria monocytogenes using immunomagnetic separation and real-time PCR assay. Abstarct accepted for presentation at the 2006 Annual Meeting of the American Society of Microbiology.
- Walker, A., M. Corley, and P.G. Reddy. 2005. Abstract submitted for presentation at the 2006 Association of Research Directors meeting, Atlant, GA.
- Reddy, P.G. Surveliinace and diagnosis of West Nile virus infections. Key Note paper submitted for presentation at the Indian Society of Veterinary Medicine, Bangalore, India, February 22-24, 2006.
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Progress 10/01/03 to 09/30/04
Outputs
A real-time PCR machine (CEPHEID) has been purchased and several graduate studens are trained to use it in their research projects. Some of the research projects assigned to the students include: rapid detection of Listeria in ready-to-eat foods, detection of West Nile virus in mosquitoes, quantitation of Th1/Th2 cytokines in animals exposed to methyl-mercury compunds and rapid detection of Campylobacter jejuni in chicken samples. Multimedia presentations with animation have been prepared for the courses of Immunology (MBIO 511), Advanced Immunology (MBIO 522), Advanced Pathogenic Microbiology (MBIO 523), and Virology & Immunology Laboratory (MBIO 521). The syllabus for course of Immunology has been made available to the students on-line through BLACKBOARD 6, a e-learning web-based software system. Two graduate students are currently receiving stipends and tuition support. Two veterinary students have completed an 8-week international research internship training at
the Faculty of Veterinary Science, Sokoine University of Agriculture, Morogoro, Tanzania and participated in a study on the prevalence of Cryptosporidium infection among domestic and wild animals in the Morogoro area. Dr. Ron Schultz and Dr. Ogi Okwumabua from the University of Wisconsin, have provided guest lectures in the above mentioned courses.
PRODUCTS: A real-time PCR machine (Smart Cycler; CEPHEID) has been purchased. Two graduate students are receiving stipends and tuition supports. Two veterinary students have spent 8-weeks in Tanzania for their international research internship. Six copies of the new edition of "Veterinary Immunology" by Tizard have been purchased for the Library. Reagents and chemicals needed for the molecular assays used in graduate student research projects have been purchased. Two graduate students have received training in molecular techniques at the University of Wisconsin.
OUTCOMES: Graduate students are currently using Smart Cycler in their research projects. Two students are expected to graduate in August 2005. Importantly, use of real-time PCR technique is being routinely used by few other graduate students. The two veterinary students that spent 8 weeks in Tanzania made a presentation on their research during the Annual Veterinary Symposium.
DISSEMINATION ACTIVITIES:
FUTURE INITIATIVES: Graduate students as well as selected veterinary students will continue to receive hands-on training in molecular techniques including real-time PCR, cloning and DNA microarray technology. They will also hands-0n training at the selected USDA laboratories. Similarly, one or two faculty members will receive hands-on training in the latest molecular techniques including reverse genetics. Problem-based teaching methodologies will be developed for the identified core courses.
Impacts
The major impact of the project is the production of master's level students and selected veterinary students very well trained in state-of-the-art molecular techniques. This is likely to attract high quality students to the Ph.D program in biosciences starting in the Fall 2005.
Publications
- No publications reported this period
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