Source: KANSAS STATE UNIV submitted to
WORKSHOP ON WHEAT GENOME SEQUENCING, NOVEMBER 2003
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
OTHER GRANTS
Reporting Frequency
Annual
Accession No.
0196750
Grant No.
2003-38836-01639
Project No.
KS9745
Proposal No.
2003-05075
Multistate No.
(N/A)
Program Code
MN.1
Project Start Date
Sep 1, 2003
Project End Date
Aug 31, 2004
Grant Year
2003
Project Director
Gill, B.
Recipient Organization
KANSAS STATE UNIV
(N/A)
MANHATTAN,KS 66506
Performing Department
PLANT PATHOLOGY
Non Technical Summary
The purpose of the proposed workshop is to establish a clear plan, utilizing the expertise from other genome sequencing projects, for the wheat genome project.
Animal Health Component
(N/A)
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20515491050100%
Knowledge Area
205 - Plant Management Systems;

Subject Of Investigation
1549 - Wheat, general/other;

Field Of Science
1050 - Developmental biology;
Goals / Objectives
Establish a dialogue between genome researchers, breeding and industry interests to explore the scientific need, relevance to cereal genome analysis and feasibility of detailed analysis of the wheat genome at the DNA sequence level. Establish a strategy for wheat genome sequencing. Establish a plan for international coordination, capturing, and dissemination of the wheat genome sequence information.
Project Methods
Investments by the USDA and NSF, together with global investments, have provided resources for the analysis of the wheat genome that make it feasible to plan the sequencing of gene-rich regions of this complex genome. The sequence database for the expressed gene sequences (ESTs) is a combination of both private and public investments and the entire database is being utilized in the public arena. Molecular and cytogenetic mapping of ESTs in chromosome bins using deletion stocks have revealed that many regions of the wheat genome appear partitioned into gene-poor and gene rich compartments. The gene-rich regions are accessible either by EST probing of large DNA (BAC) libraries or generalized genome fractionation methods for a large genome-sequencing project. The increasing efficiency and the lowering cost of large-scale genome sequencing makes it feasible to plan a project as large as sequencing the gene-rich regions of the wheat genome. This planning process, followed by the commitment to achieve the goals, is widely supported internationally. The purpose of the proposed workshop is to establish a clear plan, utilizing the expertise from other genome sequencing projects, for the wheat genome project. Scope and Dissemination: To allow focused discussions, the Workshop will be small, with fewer than 60 participants. Possible participants and speakers will have the range of expertise needed to achieve the Workshop objectives. Speakers, key participants, and funding agency representatives will be invited directly by telephone/E-mail contact with a follow-up letter of invitation and a copy of the proposed agenda. Needs, Capacity, & Benefits: The Workshop will begin with brief reviews of challenges facing wheat improvement in the 21st century. Then, to guide our discussions, part of the first day session will be devoted to scientific and technical overviews of the status of cereal genomics including both structural and functional analyses and bioinformatics, and the resources available to accomplish these efforts. Lessons Learned: Another group of presentations will include summaries of already funded or completed sequencing efforts. Speakers will present general strategies, and the important lessons learned, including pitfalls, from these projects. Focused Discussions: The morning of the second day will be spent in open but guided discussions geared to outline merit, the specific needs and strategies to accomplish wheat genome sequencing, and a plan for the next steps. Method of announcement or invitation. All participants will be contacted via electronic mail.

Progress 09/01/03 to 08/31/04

Outputs
The aim of the workshop held in Washington DC November 10 and 11, 2003, was to integrate advice from other genome projects and develop a strategy for sequencing the wheat genome. First, the workshop revisited national and international collaborative research in wheat genomics and the lessons learned from other genomics projects and model organisms. Some general concepts, including the principle that the genomes of major crops should be sequenced if we are to interpret the details of how gene networks function; that it is not possible to use rice as a surrogate for others due to the common occurrence of deletions and rearrangements when cereal genomes (rice, wheat, barley) are compared; that major differences in gene structure, expression, and function exist between cereal species; and that gene annotation of orthologous genes between species remains a major challenge. The wheat genome is likely to be the largest genome ever to be sequenced and will provide a model for changes that accompany polyploidy, a phenomenon that is common among plants.

Impacts
A wheat sequence will provide perfect markers for difficult traits, harness genetic diversity, enhance quality, increase yield in drought-prone areas, and help design varieties for sustainable food production. The benefits of a wheat sequence for human health and nutrition include enhanced nutrition especially in developing countries, world food security, and crop adaptation to climate change. The outcomes from a significant, wheat genome project will reach across the global agricultural, research, and education communities. An international collaboration of wheat researchers will meet the responsibility of providing food for much of the world's population. Scientific outcomes include tools for marker-assisted selection for wheat improvement, insights into how a polyploid genome is organized and functions, and a stimulus for further national and international collaboration.

Publications

  • Gill BS, Appels R, Botha-Oberholster A-M, Buell CR, Bennetzen JL, Chalhoub B, Chumley F, Dvorak J, Iwanaga M, Keller B, Li W, McCombie WR, Ogihara Y, Quetier F, and Sasaki T. 2004. A workshop report on wheat genome sequencing. The International Genome Research on Wheat Consortium. Genetics 168:1087-1096.


Progress 01/01/03 to 12/31/03

Outputs
The aim of the workshop held in Washington DC on November 10 and 11, 2003, was to integrate advice from other genome projects and develop a strategy for sequencing the wheat genome. The workshop revisited national and international collaborative research in wheat genomics and the lessons learned from other genomics projects and model organisms. Some general concepts, including the principle that the genomes of major crops should be sequenced if we are to interpret the details of how gene networks function; that it is not possible to use rice as a surrogate for others due to the common occurrence of deletions and rearrangements when cereal genomes (rice, wheat, barley) are compared; major differences in gene structure, expression, and function exist between cereal species; and gene annotation of orthologous genes between species remains a major challenge. The wheat genome is likely to be the largest genome ever to be sequenced and will provide a model for changes that accompany polyploidy, a phenomenon that is common among plants.

Impacts
A wheat sequence will provide perfect markers for difficult traits, harness genetic diversity, enhance quality, increase yield in drought-prone areas, and help design varieties for sustainable food production. The benefits include enhanced nutrition in developing countries, world food security, and crop adaptation to climate change.

Publications

  • No publications reported this period