Source: AUBURN UNIVERSITY submitted to NRP
DETERMINATION OF MILK WITHDRAWAL TIME OF COMMONLY USED ANESTHETICS IN DAIRY COWS
Sponsoring Institution
Cooperating Schools of Veterinary Medicine
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0196622
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2002
Project End Date
Sep 30, 2004
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
AUBURN UNIVERSITY
108 M. WHITE SMITH HALL
AUBURN,AL 36849
Performing Department
COLLEGE OF VETERINARY MEDICINE
Non Technical Summary
Not knowing correct milk withdrawal of the anesthetics can result in consumption of milk containing anesthetic residue which may greatly affects human health. Milk withdrawal time of the anesthetic can be determined based on the disappearance time of the anesthetics from the blood and the milk. The result of this project, thus, can be used to prevent anesthetic residue in the milk.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
71134501180100%
Goals / Objectives
The goals of this project are to measure the concentrations of commonly used anesthetics and anesthetic adjuncts (xylazine, butorphanol, ketamine, propofol, guaifenesin,lidocaine, tolazoline, and yohimbine) in milk over times and hence determine actual milk withdrawal times of these drugs when administered to dairy cows.
Project Methods
Nine dairy cows and nine anesthetic treatments will be studied in this projects. Cows will be randomly assigned to each treatment with at least a one-week interval between treatments. Ten milliliters each of milk and venous blood samples will be collected prior to and at 5, 10, 20, 30, 40, 50, and 60 minutes and at 2, 4, 6, 8, 10, 12, 24, 48, 72, 96 and 120 hours after anesthetic administration to determine anesthetic concentration over times. High performance liquid chromatography (HPLC) will be used for assay of anesthetic concentrations.

Progress 10/01/02 to 09/30/04

Outputs
The milk and blood samples collected for this study are currenlty still under analysis of the anesthetics with HPLC.

Impacts
The result of this study will prevent human consumption of anesthetics from the milk.

Publications

  • No pulication in year 2004 (publication the results of analysis).


Progress 01/01/04 to 09/30/04

Outputs
Collection of the blood and milk samples from 9 cows receiving 9 different treatments (Xylazine, Butorphanol, Guaifenesin, Ketamine, Propofol, Lidoacaine-epidural, Lidocaine-infiltration, Yohimbine, Tolazoline) have been completed. Currently, the HPLC is being set up and testing extracting solution with standards. Once this step is satisfactory, then we will begin with sample analysis for drug concentration for each treatment.

Impacts
The result of this study will prevent human consumption of anesthetics from the milk.

Publications

  • No publications reported this period