Progress 09/01/02 to 08/31/06
Outputs
We prepared the samples and extracted tubers of 123 genotypes in USA and Egypt, and made an initial purification through solid phase extraction columns specific for basic molecules enabling cytokinins to be separated from the non-basic plant hormones. Every sample was run through HPLC, collecting fractions for IAA, JA, tuberonic acid and tuberonic acid glucoside. The IAA samples were run on GCMS, but the data was yet been analyzed. The other samples have yet to be run. All samples were analyzed for the cytokinins zeatin, zeatin riboside, isopentenyl adenine and isopentenyl adenosine. Unfortunately, despite repeated attempts, the variability was too great to permit any definitive conclusions. No conclusive data was available at the official termination of the project. It is hoped to continue working on the samples.
Impacts
A determination of the hormonal content of potato tubers in relation to dormancy and sprouting will assist in the hormonal regulation of dormancy and sprouting
Publications
- No publications reported this period
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Progress 01/01/05 to 12/31/05
Outputs
One hundred and twenty potato tuber samples were purified by HPLC into fractions for jasmonic acid, indoleacetic acid, tuberonic acid and tuberonic acid glycoside. The jasmonic acid samples were derivatized and analyzed by GC-MS. The analysis of other samples is still to be done. Calculations of levels of jasmonic acid are ongoing. Zeatin, zeatin riboside, isopentenyl adenine and isopentenyl adenosine were analyzed in these samples by immunoassay. Analysis of the data is ongoing.
Impacts
A determination of the hormonal content of potato tubers in relation to dormancy and sprouting will assist in the hormonal regulation of dormancy and sprouting.
Publications
- Ewing, E. E., Simko, I., Omer, E. A. and Davies P. J. 2004. Polygene mapping as a tool to study the physiology of potato tuberization and dormancy. Am. J. Potato Research 81: 33-41.
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Progress 01/01/04 to 12/31/04
Outputs
The objective of this project is to identify quantitative trait loci (QTL) for plant hormones associated with potato dormancy or dormancy breaking in an RFLP-mapped potato population. 123 clones were previously mapped for the depth of tuber dormancy. Tuber samples of all 123 clones were extracted in methanol, the methanol evaporated, and the extract run through solid phase extraction (SPE) cartridges with consecutive elution solvents designed to separate acidic and basic plant hormones. The basic fraction was used to quantitate the level of zeatin/zeatin riboside and isopentenyl adenine/adenosine using enzyme linked immunosorbent assay (ELISA). The assay was performed 5 times for replication and consistency. The data are currently being computed.
Impacts
A determination of the hormonal content of potato tubers in relation to dormancy and sprouting will assist in the hormonal regulation of dormancy and sprouting.
Publications
- No publications reported this period
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Progress 01/01/03 to 12/31/03
Outputs
123 clones of RFLP mapped potato were grown harvested and the tubers placed in cold storage until the earliest started to sprout. About 10-15g of tubers was freeze dried and stored at -80C with desiccant. 63 samples were extracted and the extract partially purified. 60 samples were transported to Egypt for extraction and purification there as per the terms of the research agreement.
Impacts
A determination of the hormonal content of potato tubers in relation to dormancy and sprouting will assist in the hormonal regulation of dormancy and sprouting.
Publications
- No publications reported this period
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