Source: OREGON STATE UNIVERSITY submitted to NRP
A FLEXIBLE PROGRAM FOR INTEGRATED MANAGEMENT OF POSTHARVEST DECAY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0194042
Grant No.
2002-34103-11984
Cumulative Award Amt.
(N/A)
Proposal No.
2002-04084
Multistate No.
(N/A)
Project Start Date
May 15, 2002
Project End Date
Sep 30, 2004
Grant Year
2002
Program Code
[QQ]- (N/A)
Recipient Organization
OREGON STATE UNIVERSITY
(N/A)
CORVALLIS,OR 97331
Performing Department
SOUTHERN OREGON RESEARCH AND EXTENSION CENTER
Non Technical Summary
Postharvest fungal decay causes serious losses in the pear industry of the Pacific Northwest. Current control programs depend on postharvest application of a fungicide that is ineffective against some of the causal fungi and may be undesirable for certain markets. The purpose of this project is to evaluate control programs composed of nutritional, biological, and chemical techniques, and identify programs appropriate to various levels of disease risk.
Animal Health Component
90%
Research Effort Categories
Basic
10%
Applied
90%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21211151160100%
Knowledge Area
212 - Pathogens and Nematodes Affecting Plants;

Subject Of Investigation
1115 - Pear;

Field Of Science
1160 - Pathology;
Goals / Objectives
The objectives of this project are: (1) To validate the effectiveness of an integrated program for pear postharvest decay built of optional sequential field treatments with calcium chloride, two fungicides (ziram and trifloxystrobin) and postharvest application of yeast biocontrol agents; (2) to effectively communicate the features of this program to pear producers and packinghouse operators.
Project Methods
The concept behind this project is that different treatment options will apply to different disease risk situations and to different producer objectives. The treatment options in this program are calcium chloride as a nutritional supplement, ziram as a conventional long-residual fungicide, trifloxystrobin as a reduced-risk short-residual fungicide, and the yeast Cryptococcus infirmo-miniatus (CIM) or Candida oleophila as postharvest biocontrol treatments. The expected outcome of the research is validation of the idea that specific combinations of treatment options may be applied to fit the specific needs of the producer with respect to disease risk (based on orchard history) and the level of IPM the producer wishes to adopt. The plot will be designed factorially to include all possible combinations of field and postharvest treatment levels.

Progress 05/15/02 to 09/30/04

Outputs
In two growing seasons, randomized plots of pear trees were treated with all possible combinations of calcium chloride and the fungicides ziram and trifloxystrobin (Flint) in a factorial design. Fruit from trees receiving each possible combination of orchard treatments then received postharvest treatment with water, thiabendazole (TBZ), the yeast Cryptococcus infirmo-miniatus (CIM), or biocontrol products Aspire (2002) or BioSave (2003). Each fruit was wounded with a nail, and stored at 31 F for 3 months. Decay that developed at wounds was identified and the percentage of wounds infected was calculated. The experimental design allowed various types of decay management programs to be extracted and compared. Five program alternatives will be described. (1) No chemical treatments in orchard or postharvest resulted in 19.8 % of wounds infected (average of 2002 and 2003 results). (2) A full program of calcium and fungicide treatments in the orchard, but no postharvest treatment resulted in 5.9 % of wounds infected. (3) A full orchard program followed by postharvest biocontrol with CIM resulted in 2.8 % infection, with Aspire/BioSave resulted in 1.0 and 2.6 % infection, and with TBZ resulted in 5.3 % infection. (4) No chemical treatments in the orchard, but biocontrol postharvest resulted in 5.2 % infection with CIM and in 11.4 and 1.2 % infection with Aspire/BioSave. (5) Using only organic-compatible treatments (calcium in the orchard resulted in 8.4 % infection without postharvest treatment, and in 4.3 % with CIM, 2.0 % with Aspire, and 2.2 % with BioSave. Across all postharvest treatments, orchard treatment with calcium chloride was a significant factor in both years of study, while ziram and trifloxystrobin were significant as individual effects only in 2002. The interaction of ziram and trifloxystrobin was significant in 2003. Across all orchard treatments, all of the biocontrol agent effects were significant in both years, while the TBZ effect was not significant. These results may be used to identify decay risk levels associated with the various choices of management program presented. All programs were of value, compared with no treatments in the orchard or postharvest. Orchard treatments contributed substantially to decay control; calcium alone reduced decay while calcium followed by ziram and flint (full orchard program) resulted in less decay. Without orchard treatments, postharvest biocontrol results were variable, but the combination of orchard treatment, whether calcium alone or calcium followed by fungicides, subsequently followed by biocontrol treatments generally resulted in the least amount of infection. Programs consisting of diverse elements are likely to be more dependable in providing decay control. A minimum program of calcium in the orchard followed by postharvest biocontrol can provide substantial decay control, where avoiding use of fungicides is desired. In this study, the programs with the greatest diversity of tactics, integrating calcium, fungicides, and postharvest biocontrol, resulted in the greatest level of control.

Impacts
The results of this study can be used as an educational tool for decision-making in pear production. Pear producers can have greater awareness of the impact of their management choices on postharvest decay, and of the overall decay potential of various management strategies. This will help producers to identify risks and consider optimum practices within a market-based production strategy. Where no fungicide use is preferred, or where fungicide use is to be limited to the orchard, programs evaluated in this project provided reasonable levels of decay control. A program compatible with organic standards, using calcium in the orchard and biocontrol agents postharvest, provided substantial decay control as compared with taking no action against decay. Maximum decay control was achieved with a combination of nutritional enhancement, orchard fungicide sprays, and postharvest fungicide applications. This integrated program should provide dependable control, as it consists of diverse tactics acting to reduce decay. Economic benefits should accrue from identifying the optimum decay control program that is compatible with production and market strategies.

Publications

  • No publications reported this period


Progress 01/01/03 to 12/31/03

Outputs
Without any orchard or postharvest treatments, fungal decay developed in approximately 17% of Bosc pears held for five months in cold storage. Application of calcium in the orchard ("no fungicide" or organic program) reduced decay to 13%, and application of fungicides in the orchard reduced decay to 6%. In each type of orchard program, postharvest application of biocontrol agents further reduced decay. Using a combination of orchard fungicides and postharvest application of biocontrol agents, decay was less than 1%, while the "no fungicide" program of calcium followed by biocontrol resulted in 3-4% decay. Decay levels achieved by all treatment combination choices were defined.

Impacts
Pear producers can have greater awareness of the impact of their management choices on postharvest decay, and of the overall decay potential of various management strategies. This will help producers to identify risks and consider optimum practices within a market-based production strategy. Where no fungicide use is preferred, or where fungicide use is to be limited to the orchard, programs evaluated in this project can provide reasonable levels of decay control. However, maximum decay control was achieved with a combination of nutritional enhancement, orchard fungicide sprays, and postharvest fungicide applications.

Publications

  • No publications reported this period


Progress 05/15/02 to 12/31/02

Outputs
Sequential treatments were applied to Bosc pear trees in a factorial completely randomized design during the summer and fall of 2002. Fruit calcium enrichment via three orchard sprays of calcium chloride in mid-summer, ziram fungicide applied one month before harvest, and tryfloxystrobin two weeks before harvest were applied in all possible factorial arrangements, including untreated. After harvest, a portion of the fruit harvested from each replicate of each treatment were artificially wounded, and all fruit received a simulated packinghouse line-spray of either the yeast Cryptococcus infirmo-miniatus, the yeast biological control product Aspire, the fungicide thiabendazole, or no treatment. All fruit are currently in storage at 0 C awaiting decay development. Results from various treatment combinations reflecting different management and marketing strategies will be compared.

Impacts
It is expected that this project will demonstrate the relative effectiveness of various control tactic combinations, in turn facilitating producer choice among decay management strategies. Different decay control strategies could be applied to satisfy diverse marketing strategies (organic, conventional, or specific export requirements). Overall, the project results should indicate whether or not successful pear storage decay can be achieved without the postharvest application of fungicide, or if control provided by traditional postharvest fungicide treatments can be improved upon by combination with orchard tactics. Furthermore, results should indicate the relative value of management programs without any fungicide treatments (calcium in the orchard + yeast postharvest). If found to be viable alternatives, these programs would potentially reduce environmental impact through reduced fungicide residue on fruit and elimination of fungicide runoff and disposal in pear packinghouses.

Publications

  • No publications reported this period