Progress 01/01/03 to 12/31/03
Outputs
The SF4 gene conferring seedlessness with NPTII reporter gene was genetically engineered into the muscadine "Fry" embryogenic cell lines via the Agrobacterium system. Putative transgenic plantlets have been recovered. PCR analysis was used to verify the true transgenic grapes. The transgenic grapes were being raised in the greenhouse. In the mean time, experiments on improving the transformation and regeneration efficiency are continuing. Over 8,000 flowers of seedless females were pollinated for the seedless breeding project. About thirty putative seedless hybrids of bunch grapes x muscadine grapes were produced by embryo rescue. Fifteen Euvitis x Muscadinia hybrids were identified and confirmed from crosses made in previous years. These hybrids have been established in the field, and some of which were used for backcross pollination in the 2003 season.
Impacts
Diseases limit the production of Vitis vinifera L., a predominant commercial table grape species with many seedless cultivars in the Southeastern United States. No grapes in commercial production in the southeast are seedless. Incorporating the "seedless factors" into the seeded grapes to convert them into seedless ones will have great economic potential for this region. Five hundred percent increase in acreage within three years is expected should a seedless muscadine cultivar is developed for commercial use.
Publications
- No publications reported this period
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Progress 09/01/00 to 08/31/03
Outputs
The SF4 gene conferring seedlessness with NPTII reporter gene was genetically engineered into the muscadine "Fry" embryogenic cell lines via the Agrobacterium system. Twenty putative transgenic plantlets have been transferred, established and maintained in 5 gallon pots in the greenhouse. PCR and southern analysis was conducted to verify the true transgenic grapes. The seediness/seedlessness status of these grapes have not been confirmed since the putative transgenic grapes have not bloomed. In the mean time, we have produced three more embryogenic lines of muscadine grapes ("Tara", "Alachua", and "Triumph"). Experiments on improving transformation and regeneration efficiency of muscadine grapes are continuing. We have also successfully used embryo rescue technique for introgression of the seedlessness genes from V. vinifera grape varieties into the muscadine grapes. About thirty V. rotundifolia V. vinifera hybrids have been recovered from twenty cross combinations and
over 20,000 flowers. These hybrids have been established in the field, some of which appeared seedless, including both stenospermic and parthonocarpic seedless. Some of the hybrids have been backcrossing with either muscadine or seedless V. vinifera parents.
Impacts
Diseases are the limiting factor for the production of Vitis vinifera grape in the Southeastern United States. No grapes in commercial production in the southeast are seedless. Incorporating the "seedless factors" into seeded grapes to convert them into seedless ones will have great economic potential in this region. Five hundred percent increase in acreage within three years is expected if a seedless muscadine cultivar is developed for commercial use.
Publications
- Colova-Tsolova, V., J. Lu and A. Perl. 2002. Cyto-embryological aspects of seedlessness in Vitis vinifera L. and exploiting DNA recombinant technology as an advanced approach for inducing seedlessness in European and muscadine grapes. J.ISHS. In press.
- X. Xu and J. Lu. 2002. Cytogenetic study of interspecific hybrids between Vitis rotundifolia and Vitis vinifera. J. ISHS.
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Progress 01/01/02 to 12/31/02
Outputs
The SF4 gene conferring seedlessness with NPTII reporter gene was genetically engineered into the muscadine "Fry" embryogenic cell lines via the Agrobacterium system. Putative transgenic plantlets have been recovered. PCR and Southern analysis are being used to confirm the true transgenic grapes. In the mean time, experiments on improving the transformation and regeneration efficiency are continuing. Over 10,000 flowers of seedless females were pollinated for the seedless breeding project. About thirty five putative seedless hybrids of bunch grapes x muscadine grapes were produced by embryo rescue. Over a dozen Euvitis x Muscadinia hybrids were identified and confirmed from crosses made in previous years. These hybrids have established in the field, and some of which are expected to bloom in 2003 season.
Impacts
Diseases limit the production of Vitis vinifera L., a predominant commercial table grape species with many seedless cultivars grown World wide. No grapes in commercial production in the southeast are seedless. Five hundred percent increase in acreage within three years is expected should a seedless muscadine cultivar is developed for commercial use.
Publications
- V. Colova-Tsolova, L. Lu and A. Perl. 2002. Cyto-embryological aspects of seedlessness in Vitis vinifera L. and exploiting DNA recombinant technology as an advanced approach for inducing seedlessness in European and muscadine grapes. J.ISHS. In press.
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Progress 01/01/01 to 12/31/01
Outputs
Embryogenic cell lines have been established for muscadine grape cultivar "Fry". Through a series of experiments, regeneration has been achieved from cell suspension of the muscadine embryogenic cells. In the mean time, a transformation system has been developed using the somatic cell lines and reporter genes via the Agrobacterium system. The SF4 gene conferring seedlessness with NPTII reporter gene was genetically engineered into the muscadine "Fry" embryogenic cell lines. Recovering plantlets from the putative transgenic cells proved to be difficult. Experiments on stimulating germination of the putative emryogenic lines were under way. Two hundred and twenty putative seedless hybrids were obtained from embryo rescue. Among them, twenty were the interspecific hybrids of bunch grapes x muscadine grapes. Several back-crossed hybrids [(seedless V. vinifera x muscadine) x muscadine] with seedless potential were produced and established in the field.
Impacts
Diseases limit the production of Vitis vinifera L., a predominant commercial table grape species with many seedless cultivars in the Southeastern United States. No grapes in commercial production in the southeast are seedless. Incorporating the "seedless factors" into the seeded grapes to convert them into seedless ones will have great economic potential for this region. Five hundred percent increase in acreage within three years is expected should a seedless muscadine cultivar is developed for commercial use.
Publications
- Colova, V. & J. Lu. 2001. DNA recombinant technology as a efficient tool to express alien gene in grape: genetically engineered grape for seedless and stress tolerance. ASEV Annual Meeting, San Diego, California.
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