Source: UNIVERSITY OF NEVADA submitted to
AGREEMENT BETWEEN SIERRA BIOMEDICAL RESEARCH CORP AND THE BOARD OF REGENTS OF THE UCCSN ON BEHALF OF THE UNIVERSITY OF NEVADA, RENO
Sponsoring Institution
State Agricultural Experiment Station
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0192837
Grant No.
(N/A)
Project No.
NEV053AC
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Apr 1, 2002
Project End Date
Aug 31, 2008
Grant Year
(N/A)
Project Director
Zanjani, E.
Recipient Organization
UNIVERSITY OF NEVADA
(N/A)
RENO,NV 89557
Performing Department
Ag Nutrition and Vet Sciences
Non Technical Summary
(N/A)
Animal Health Component
(N/A)
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
30536101020100%
Knowledge Area
305 - Animal Physiological Processes;

Subject Of Investigation
3610 - Sheep, live animal;

Field Of Science
1020 - Physiology;
Goals / Objectives
Support salary for Research Associate working in th Research Laboratory of Esmail Zanjani. Research consists of flow cytometry on the FACScan, cell/tissue culture from human and animal samples, cell sorting on the FACS Vantage and basic alb maintenance/management.
Project Methods
Basic maintenance including quality control and cleaning. Preparing and staining cell suspensions. Culturing of cells in varioius media. Maintenance of cell sorter including washing, blaching, alcohol on a weekly or as needed basis. Preparing, aliquotting and freezing media. Inventory of supplies. Assisting physicians with bone marrow aspirations.

Progress 04/01/02 to 08/31/08

Outputs
OUTPUTS: This agreement which provided salary suport for a research associate working in the research laboratory of Esmail D. Zanjani, expired on March 31, 2008. Funds have been exhausted and the corresponding account has been closed. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

Impacts
The research associate funded by this agreement was responsible for flow cytometry on BD FacScan and FacsVantage equipment, cell/tissue culture from human and animal samples. Basic maintenance including quality control and cleaning, preparing and staining cell suspensions, and culturing of cells in various media. Maintained inventory of supplies used in labs. Assisted physicians with bone marrow aspirations used in research.

Publications

  • No publications to report for this agreement in 2008.


Progress 01/01/07 to 12/31/07

Outputs
This agreement provides salary support for a research associate working in the research laboratory of Esmail D. Zanjani. Research consists of flow cytometry on the FACScan, cell/tissue culture from human and animal samples, cell sorting on the FACS Vantage and basic maintenance/management.

Impacts
Basic maintenance including quality control and cleaning, preparing and staining cell suspensions, and culturing of cells in various media. Maintains cell sorter including washing, bleaching, and cleaning with alcohol on a weekly or as needed basis. Prepares and aliquots freezing media. Responsible for maintaining inventory of supplies. Assists physicians with bone marrow aspirations used in research.

Publications

  • No publications reported this period


Progress 01/01/06 to 12/31/06

Outputs
OBJECTIVES: Support salary for Research Associate working in the Research Laboratory of Esmail D. Zanjani. Research consists of flow cytometry on FACScan, cell/tissue culture from human and animal samples, cell sorting on the FACS Vantage and basic laboratory maintenance/management. APPROACH: Basic maintenance including quality control and cleaning. Preparing and staining cell suspensions. Culturing of cells in various media. Maintenance of cell sorter including washing, bleaching, alcohol on a weekly or as needed basis. Preparing, aliquotting and freezing media. Inventory of supplies. Asssisting physicians with bone marrow asipirations. KEY WORDS: Sheep; animal physiology; flow cytometry; tissue culture; cell culture; agreements; research; quality control; research equipment; research services; maintenance; culture media; staining; inventory; bone marrow aspiration PROGRESS; Sierra Biomedical Research Corporation provides funds through this agreement to pay for the salary and fringe benefits of Donna Colon to the UNR Board of Regents.

Impacts
Ms. Colon continues to work on the animal surgery projects. Her assistance is essential to work being done in Dr. Zanjani's laboratory.

Publications

  • Jacoub JF, Suryadevara U, Pereyra V, Colon D, Fontelonga A, Mackintosh FR, Hall SW, Ascensao JL. Mobilization strategies for the collection of peripheral blood progenitor cells: Results from a pilot study of delayed addition G-CSF following chemotherapy and review of the literature. Exp Hematol. 2006 Nov;34(11):1443-50.


Progress 01/01/05 to 12/31/05

Outputs
OBJECTIVES: Support salary for Research Associate working in the Research Laboratory of Esmail D. Zanjani. Research consists of flow cytometry on FACScan, cell/tissue culture from human and animal samples, cell sorting on the FACS Vantage and basic laboratory maintenance/management. APPROACH: Basic maintenance including quality control and cleaning. Preparing and staining cell suspensions. Culturing of cells in various media. Maintenance of cell sorter including washing, bleaching, alcohol on a weekly or as needed basis. Preparing, aliquotting and freezing media. Inventory of supplies. Asssisting physicians with bone marrow asipirations. KEY WORDS: Sheep; animal physiology; flow cytometry; tissue culture; cell culture; agreements; research; quality control; research equipment; research services; maintenance; culture media; staining; inventory; bone marrow aspiration PROGRESS; Sierra Biomedical Research Corporation provides funds through this agreement to pay for the salary and fringe benefits of Donna Colon to the UNR Board of Regents.

Impacts
Ms. Colon continues to work on the animal surgery projects. Her assistance is essential to work being done in Dr. Zanjani's laboratory.

Publications

  • Narayan AD, Ersek A, Campbell TA, Colon DM, Pixley JS, Zanjani ED. The effect of hypoxia and stem cell source on haemoglobin switching. Br J Haematol. 2005 Feb;128(4):562-70.


Progress 01/01/04 to 12/31/04

Outputs
OBJECTIVES: Support salary for Research Associate working in the Research Laboratory of Esmail D. Zanjani. Research consists of flow cytometry on FACScan, cell/tissue culture from human and animal samples, cell sorting on the FACS Vantage and basic laboratory maintenance/management. APPROACH: Basic maintenance including quality control and cleaning. Preparing and staining cell suspensions. Culturing of cells in various media. Maintenance of cell sorter including washing, bleaching, alcohol on a weekly or as needed basis. Preparing, aliquotting and freezing media. Inventory of supplies. Asssisting physicians with bone marrow asipirations. KEY WORDS: Sheep; animal physiology; flow cytometry; tissue culture; cell culture; agreements; research; quality control; research equipment; research services; maintenance; culture media; staining; inventory; bone marrow aspiration PROGRESS; Sierra Biomedical Research Corporation provides funds through this agreement to pay for the salary and fringe benefits of Donna Colon to the UNR Board of Regents.

Impacts
Ms. Colon works on the animal surgery projects. Her assistance is essential to the work being done in Dr. Zanjani's laboratory.

Publications

  • 2004/01 to 2004/12. No publications reported this period.


Progress 01/01/03 to 12/31/03

Outputs
This agreement is the to pay the salary for a Research Associate.

Impacts
See report.

Publications

  • No publications reported this period


Progress 01/01/02 to 12/31/02

Outputs
Sierra Biomedical Research Corporation provides funding for the salary and fringe benefits of Ms. Donna Colon to the UNR Board of Regents under this agreement.

Impacts
Ms Colon works on the animal surgery project. Her assistance is essential to the work being done in Dr Zanjani's lab.

Publications

  • No publications reported this period