Source: EMBREX, INC. submitted to NRP
AUTOMATED SYSTEM FOR DETECTION AND REMOVAL OF NON-VIABLE POULTRY EGGS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0190882
Grant No.
2001-33610-11082
Cumulative Award Amt.
$243,832.00
Proposal No.
2001-03219
Multistate No.
(N/A)
Project Start Date
Sep 1, 2001
Project End Date
Aug 31, 2004
Grant Year
2001
Program Code
[(N/A)]- (N/A)
Recipient Organization
EMBREX, INC.
1040 Swabia Ct.
DURHAM,NC 27703
Performing Department
(N/A)
Non Technical Summary
In the US about 90% of the ten-billion broiler chicks hatched annually are vaccinated against various diseases by placing vaccine in the egg three days before the chick hatches. There is a need in the poultry industry for an automated system that will reliably discriminate between live and dead eggs during incubation to allow removal of all dead and infertile eggs prior to egg inoculation and hatching. The incubation of dead, contaminated eggs can contaminate incubators and in ovo injection systems and spread disease agents to other eggs. Removing non-viable eggs improves the operation of conventional incubators by increasing the number of live eggs per incubator and protecting hatchlings from exposure to the pathogens in rotten eggs. Reduction in contaminates and pathogens from incubators and in ovo injection machinery also will reduce the need for antibiotics in hatcheries. The living embryos in live eggs produce heat so that a live egg is about 1 degree C warmer than a non-live egg. Egg temperature can be measured by non-contact infrared radiation sensors. A Thermal Candling System combines information from conventional opacity candling with egg temperature data to select live eggs both faster and more accurately than current manual or automatic systems used by the poultry industry. Commercial Thermal Candlers will be marketed as stand-alone devices and as an integral part of an in ovo vaccination systems.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3013299102050%
3013299202050%
Goals / Objectives
There is a need in the poultry industry for an automated system that will reliably discriminate live from non-live eggs during incubation to allow removal of all dead, rotten and infertile eggs prior to egg inoculation and hatching. The incubation of dead, contaminated eggs can contaminate incubators and in ovo injection systems and spread disease agents to other eggs. Removing non-viable eggs improves the operation of conventional incubators by increasing the number of live eggs per incubator and protecting hatchlings from exposure to the pathogens in rotten eggs. A Thermal Candling System combines information from conventional opacity candling with egg temperature data to select live eggs. The objective of this research is first to develop a Thermal Candling System with a sufficiently low error rate that will operate in the environment of a poultry hatchery. And second to design, build and test a fully automatic Thermal Candler capable of detecting and loading live eggs into an egg injection system meeting hatchery speed, accuracy and sanitation requirements
Project Methods
The Phase II development will begin with the purchase of an IR camera and computer interface to both understand the occasional egg temperature measurement errors that occur using IR sensors, as well as evaluate the camera's suitability for use on the machine measuring the temperatures of groups of eggs. This may apply to measuring all the bottom-egg temperatures to do live/non-live separation or the lower accuracy measurements of the tops of eggs to identify live (warm) eggs that are upside-down. This work will require using the camera with the Phase I Thermal Candler prototype in order to identify eggs with incorrect temperature data and then observe the radiation patterns across the egg. Data from these thermal scan experiments will describe the size and location of the point on the egg that will give accurate temperature data and determine the specifications for the sensor system necessary. We anticipate this system will use a 20 millisecond bolometer with lensing as it's backbone and, if necessary, a mechanical scanning system. Development of this system, testing and verification will be the major focus for the first year. During the second year we will use this sensor system to design and build a Live Loader. The Live Loader is a device that will send only live eggs to an Inovoject injection or gender sorting system. The Embrex gender sorting system is currently in development as a USDA Phase II SBIR. Because of the difficulty of removing rotten eggs from a flat, the Live Loader will likely remove the live eggs from a flat and place them in an injection support. The injection support may be another flat, or a system of cups that conveys eggs to an injection site, followed by release of the eggs into a transfer basket. (A similar system will be used for gender sorting eggs in which an allantois sampling system will penetrate the egg shell rather than injection.) Backfilling so that all positions of the gender sampling system have a live egg may also be a requirement of this application of the Live Loader. Technical challenges of the Live Loader should be the good application of mechanical engineering to create a functional and hatchery robust unit.

Progress 10/01/03 to 09/30/04

Outputs
In the US, about 90 % of the ten-billion broiler chicks hatched annually are vaccinated against various diseases by placing vaccine in the egg three days before the chick hatches. While some non-viable eggs (infertiles, early deads) can be removed before inoculation with the use of conventional opacity candling, other non-viable eggs (middle deads, rots) remain and are not only inoculated but placed into the hatcher as well. The inoculation and incubation of dead, contaminated eggs can contaminate incubators and in ovo injection systems and can support the spread of disease agents to other eggs. Removing non-viable eggs prior to inoculation improves the operation of conventional incubators by increasing the number of live eggs per incubator and protecting hatchlings from exposure to the pathogens in rotten eggs. Cleaner incubators and reduction in pathogens (and, therefore, reduced clean-up time and waste disposal), as well as reducing dependency on antibiotics during inoculation fostered interest in a system that can reliably differentiate between live and dead eggs. The living embryos in live eggs produce heat so that these eggs are warmer than non-live eggs. With the use of non-contact infrared radiation sensors or cameras, the egg temperature can be measured, recorded and analyzed. Combining information from conventional opacity candling system with egg temperature data, allows the industry to select live eggs more accurately than current manual or automatic systems used by the poultry industry. During Phase I of this SBIR grant, we were able to prove the feasibility of using non-contact egg temperature measurement for thermal candling. The purpose of the Phase II research was to eliminate problems that arose in Phase I, such as sporadic inaccurate temperature measurements and misidentification of live eggs in warm environments, and to develop a method to correctly identify upside down placed eggs. We also addressed issues that would be important for a commercial device such as protecting the sensitive thermal equipment, increasing the speed of the Thermal Candler and the accuracy with which Day 16 and Day 18 eggs are categorized as either dead or live. Using a thermal camera and image analysis tools, we were able to address all the above listed problems while significantly increasing the accuracy of the Thermal Candler. We tested 28,800 Day 18 broiler eggs of both prime and old flocks (33 and 51 weeks), as well as 9,600 Day 16 (Day 15.5) broiler eggs of a prime flock (33 weeks) in a commercial hatchery. Flock age proved to be non-detrimental to the live/dead decision-making. The automated Thermal Candler Prototype that was built during Phase II achieved accuracies around 99.9 % for both Day 18 and Day 16 eggs, including correctly identifying upside down placed eggs.

Impacts
Reducing exposure of hatchlings to contaminants such as bacteria and mold will increase hatchability and therefore increase product yield. We expect that value trials will support results from previous `manual candling and removing non-viable egg' tests, which ended with a 1.2 % to 2.0 % hatch improvement for older flocks (> 54 weeks). The United States poultry industry would receive a direct value resulting from this hatch improvement in the order of 0.2 to 0.4 cents per chick. Cleaner setters, therefore reduced clean-up time, and reduced wet waste disposal is another major benefit.

Publications

  • No publications reported this period


Progress 10/01/03 to 08/31/04

Outputs
Target Audience:nullChanges/Problems:nullWhat opportunities for training and professional development has the project provided?In the US, about 90 % of the ten-billion broiler chicks hatched annually are vaccinated against various diseases by placing vaccine in the egg three days before the chick hatches. While some non-viable eggs (infertiles, early deads) can be removed before inoculation with the use of conventional opacity candling, other non-viable eggs (middle deads, rots) remain and are not only inoculated but placed into the hatcher as well. The inoculation and incubation of dead, contaminated eggs can contaminate incubators and in ovo injection systems and can support the spread of disease agents to other eggs. Removing non-viable eggs prior to inoculation improves the operation of conventional incubators by increasing the number of live eggs per incubator and protecting hatchlings from exposure to the pathogens in rotten eggs. Cleaner incubators and reduction in pathogens (and, therefore, reduced clean-up time and waste disposal), as well as reducing dependency on antibiotics during inoculation fostered interest in a system that can reliably differentiate between live and dead eggs. The living embryos in live eggs produce heat so that these eggs are warmer than non-live eggs. With the use of non-contact infrared radiation sensors or cameras, the egg temperature can be measured, recorded and analyzed. Combining information from conventional opacity candling system with egg temperature data, allows the industry to select live eggs more accurately than current manual or automatic systems used by the poultry industry. During Phase I of this SBIR grant, we were able to prove the feasibility of using non-contact egg temperature measurement for thermal candling. The purpose of the Phase II research was to eliminate problems that arose in Phase I, such as sporadic inaccurate temperature measurements and misidentification of live eggs in warm environments, and to develop a method to correctly identify upside down placed eggs. We also addressed issues that would be important for a commercial device such as protecting the sensitive thermal equipment, increasing the speed of the Thermal Candler and the accuracy with which Day 16 and Day 18 eggs are categorized as either dead or live. Using a thermal camera and image analysis tools, we were able to address all the above listed problems while significantly increasing the accuracy of the Thermal Candler. We tested 28,800 Day 18 broiler eggs of both prime and old flocks (33 and 51 weeks), as well as 9,600 Day 16 (Day 15.5) broiler eggs of a prime flock (33 weeks) in a commercial hatchery. Flock age proved to be non-detrimental to the live/dead decision-making. The automated Thermal Candler Prototype that was built during Phase II achieved accuracies around 99.9 % for both Day 18 and Day 16 eggs, including correctly identifying upside down placed eggs.How have the results been disseminated to communities of interest?nullWhat do you plan to do during the next reporting period to accomplish the goals?null

Impacts
What was accomplished under these goals? Reducing exposure of hatchlings to contaminants such as bacteria and mold will increase hatchability and therefore increase product yield. We expect that value trials will support results from previous `manual candling and removing non-viable egg tests, which ended with a 1.2 % to 2.0 % hatch improvement for older flocks (> 54 weeks). The United States poultry industry would receive a direct value resulting from this hatch improvement in the order of 0.2 to 0.4 cents per chick. Cleaner setters, therefore reduced clean-up time, and reduced wet waste disposal is another major benefit.

Publications

  • Citation: No publications reported this period


Progress 10/01/00 to 09/30/01

Outputs
A "Thermal Marker" that could identify eggs at a rate of 22,000 eggs/hour was completed in July 2001. This research concluded with a reliability of 99.31 % correctly identified live eggs and 98.97 % correctly identified non-viable eggs. The causes of the misclassifications were unknown but hypothesized to be due to a variety of temperature measurement errors related to spot size, egg position and alignment. With those results we demonstrated the feasibility of the concept although a successful commercial machine must not discard so many live eggs. Since thermal imaging offers detailed pictures of the eggs it is a great opportunity to develop solutions to the technical challenges that arose from Phase I. We have used a thermal camera to collect data in numerous experiments and analysis of that database is providing us insight into the 1% of the eggs that are misclassified. Our candling includes the recording thermal and opacity data for each egg, as well as manual candling results for cross-referencing. We have found that there is still an occurrence of low temperature eggs that have been manually identified as live. Analysis of the thermal images shows these eggs are clearly cooler than surrounding live eggs and not cooler due to artifacts such as feathers on eggs or displaced air cells. We have followed colder live eggs through to hatch and found that of 29 "cool live" eggs in a batch of 2,700 candled eggs that only 11 hatched as healthy chicks. In response to our discoveries of "cool live" eggs, our current experiments include real time image processing to improve our sampling accuracy and make experimental results quickly available to match with eggs. Low temperature areas such as the ones resulting from aircells of upside down eggs or from feathers on the egg shell are therefore excluded in the averaging of each egg temperature. Alignment problems should also be eliminated since the software will focus on high intensity areas rather than on areas that are tied to certain coordinates. We are using our database of thermal images to develop the algorithms and older data will first be cross-checked to assure proper functioning of the software.

Impacts
The ability to detect and remove dead and rotten eggs from hatcheries will bring multiple benefits including increased hatch rates from pathogen reduction in incubators and the egg innoculation process, improved airflow in incubators, and better hatch prediction.

Publications

  • No publications reported this period