Source: NORTH CAROLINA STATE UNIV submitted to NRP
GENETIC AND PRODUCTION ENVIRONMENT INFLUENCES ON PROCESSING AND PLANTING QUALITY OF NUTRITIONALLY ENHANCED SOYBEAN SEED
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
EXTENDED
Funding Source
Reporting Frequency
Annual
Accession No.
0189407
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2001
Project End Date
Sep 30, 2008
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
NORTH CAROLINA STATE UNIV
(N/A)
RALEIGH,NC 27695
Performing Department
CROP SCIENCES
Non Technical Summary
The purpose of this project is to investigate production, harvest, and post harvest handling of soybean seed from varieties with enhanced oil and or protein content and to determine if genetically altering oil or protein content through traditional or molecular breeding techniques will have any influence on seed germination, vigor, or field performance.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20518201020100%
Knowledge Area
205 - Plant Management Systems;

Subject Of Investigation
1820 - Soybean;

Field Of Science
1020 - Physiology;
Goals / Objectives
1. Determine the influence of temperature during seed fill on the planting and nutritional quality of enhanced soybean. 2. Identify field production systems suitable for the development of high quality nutritionally enhanced soybean seeds. 3. Determine if genetic changes in soybean seed oil and protein composition influences seed quality germination and vigor and storability.
Project Methods
This project is designed to provide information about how environment during seed development, after maturity, and following storage influences nutritional and planting quality of enhanced soybean varieties developed through traditional and molecular breeding techniques. Experiments will be conducted in controlled environment chambers NCSU phytotron and in the field. Field environments will be modified by altering planting date to provide variations in day length, temperature, and humidity during critical times of soybean development and altering harvest date to evaluate field weathering. Seeds will also be stored in several environments to provide information about shelf life expectation of enhanced germplasm.

Progress 10/01/05 to 09/30/06

Outputs
Field studies on the influence of the high oleic acid trait on peanut seed quality were continued in 2006. Normal and high oleic acid peanut cultivars were harvested near maturity and separated into maturity categories. Seeds will be analyzed for seed germination, seed vigor, and fatty acid composition. Additional peanuts were harvested at maturity, shelled and placed in storage at 0, 10, and 25C. Samples will be taken every 4 months and seed germination, vigor and oil composition will be analyzed.

Impacts
The data generated from this study will provide information related to peanut seed quality as influenced by the high oleic acid trait incorporated into existing peanut cultivars.

Publications

  • No publications reported this period


Progress 10/01/04 to 09/30/05

Outputs
Field studies of the influence of high oleic acid on peanut seed quality were conducted in 2005. Peanuts were planted in May and June and harvested at maturity and 3 weeks past maturity. Our data shows that the high-oleate trait reduced seed vigor in several cultivars. The high oleic acid lines of the commercial cultivars NC 10C, NC-V11, NC 12C and Gregory all exhibited lower cool germination (18C) and higher electrical conductivity values than their paired normal oleic acid cultivars. NC 7 and NC 9 high oleic acid and normal oleic acid lines showed no difference in seed quality for the production environments investigated. Additional field studies will be established in 2006.

Impacts
Genetically altering seed oil composition has been shown to reduce seed quality of some soybean and peanut cultivars. This is especially true when seed production environments are less that favorable. Our data indicates that high-oleate soybean and peanut seed production may need to be shifted to locations where temperatures are more favorable.

Publications

  • No publications reported this period


Progress 10/01/03 to 09/30/04

Outputs
The second field study to evaluate the influence of planting date, planting pattern, and harvest date on seed quality of high oleic soybeans was completed in 2004. Laboratory analysis of seed quality (germination and vigor) and oil quality will be completed in 2005. Results from the 2003 field study suggest that delaying planting and delaying harvest of seed carrying the high oleic trait can decrease seed vigor. Seed oil composition in the 2003 study was not affected by planting date or harvest date. A seed storage study will be initiated in January 2005 with high oleic and traditional varieties. The objective will be to determine if genetic changes in soybean seed oil and protein composition influences seed storability. Seeds from traditional, high protein, and high oleic acid will be stored in temperatures of 0, 10, and 25C and evaluated after 3, 6, 9, and 12 months of storage for oil, protein and seed quality (germination and vigor).

Impacts
While the high oleic trait is enhanced when seeds are grown in a warm environment, seed quality (germination and vigor) in these same environments can be reduced. This project will provide information about seed production environment requirements of high oleic soybean seeds.

Publications

  • No publications reported this period


Progress 10/01/02 to 09/30/03

Outputs
The second replication of a phytotron study to evaluate the influence of temperature during seed-fill on oil and seed quality of high oleic soybeans was completed in 2003. Preliminary results indicate seed quality (germination and vigor) of the high oleic acid varieties is more sensitive to production temperature than traditional varieties. The high oleic acid trait normally seen in storage lipids of the cotyledons of enhanced varieties, was also expressed in the membrane lipids of the embryonic axes. One might speculate that altering membrane lipid composition could be related to increased axes membrane permeability, and therefore impact seed quality. Further studies are needed to confirm this. A field study was initiated in 2003, evaluating planting pattern, planting date, and harvest date influence on oil and seed quality of enhanced soybeans. Laboratory analysis and a second field study will be completed in 2004.

Impacts
While the high oleic trait is enhanced when seeds are grown in a warm environment, seed quality (germination and vigor) in these same environments can be reduced. This project will provide information about seed production environment requirements of high oleic soybean seeds.

Publications

  • No publications reported this period


Progress 10/01/01 to 09/30/02

Outputs
Six soybean cultivars were planted in April, May and June of 2001 and 2002. Included were traditional, high oil, high protein, and high oleic acid genotypes. In 2001, soybeans were harvested at harvest maturity, and at two-week intervals for eight weeks. Germination and electrical conductivity were conducted to measure seed quality. Oil quality was also analyzed. When averaged across varieties, seed germination and vigor increased as planting date was delayed. Germination and vigor decreased as harvest was delayed past harvest maturity. Neither planting date, nor harvest date impacted oil quality. In 2002, harvests are being conducted as close to two-week intervals as the weather would allow. Seed and grain quality will be evaluated in January and February of 2003.

Impacts
Provide information about the performance of enhanced soybean varieties under various production environments.

Publications

  • No publications reported this period


Progress 10/01/00 to 09/30/01

Outputs
The first replication of a phytotron study to evaluate the influence of temperature during seed fill on nutritionally enhanced soybean quality was completed in 2001. A second replication is planned for 2002. Preliminary results indicate seed germination and vigor of the high oleic acid varieties and breeding lines are sensitive to production temperature. This could be related to increased axes membrane permeability. A field study was initiated in 2001, evaluating planting and harvest date influence on quality of nutritionally enhanced soybeans. Laboratory analysis will be completed in 2002. A second field study will be planted in 2002.

Impacts
Data should enhance our understanding of how altering soybean oil and fatty acid content influences seed quality.

Publications

  • No publications reported this period