Progress 10/01/10 to 09/30/15
Outputs
Target Audience:Other scientists Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The PI supported by AES trained one graduate student and 2 undergraduate students and 3 postdocs How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?The projectwas terminated in 2015.
Impacts
What was accomplished under these goals?
The goal of this project is to understand the molecular mechanism underlying plant responses to stress conditions. The PI supported by AES funding directed the research effort that leads to the following results: 1. Identified a molecular pathway that mediates crosstalk of ABA signaling with RALF peptide signaling 2. Identified a molecular pathway that mediates plant tolerance to serpentine soil
Publications
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Progress 10/01/13 to 09/30/14
Outputs
Target Audience: Other scientists in plant biology community through publications, meetings, lectures. Graduate students and undergraduate students through regular university teaching. Also training,mentoring high school students through lab training and internship. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? Graduate students and undergraduate students were trained in molecular genetics, cell biology, and genomics. High school students were trained through Biotech Partnership. Postdoctoral fellows were trained to be ready for taking new independent job positions. How have the results been disseminated to communities of interest? Through publications of research articles, lectures at other universities, speaches at international meetings. What do you plan to do during the next reporting period to accomplish the goals? Continue to use molecular, cell, and genetic tools to study model plants such as Arabidopsis and rice in hte context of stress responses to identify genes that participate in plant toelrance to stressful conditions.
Impacts
What was accomplished under these goals?
1. Drought responses were studied and plant hormone ABA and its receptor identified as critical components in plant tolerance to drought. 2. Seed size control was studied and a receptor-like kinase identified to be important for control of seed size. 3. Calcium signaling pathways were studied in response to various stress signals. Several important genes were identified to be critical for the signaling pthways triggered by stress signals.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Lee SC, Lim CW, Lan W, He K, Luan S. (2013) ABA signaling in guard cells entails a dynamic protein-protein interaction relay from the PYL-RCAR family receptors to ion channels. Mol Plant. 6(2):528-38.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Zhou L, Lan W, Jiang Y, Fang W, Luan S (2014) A calcium-dependent protein kinase interacts with and activates a calcium channel to regulate pollen tube growth. Mol Plant. 7(2):369-76.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Yu F, Li J, Huang Y, Liu L, Li D, Chen L, Luan S (2014) FERONIA receptor kinase controls seed size in Arabidopsis thaliana. Mol Plant. 2014 May;7(5):920-2.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Hou C, Tian W, Kleist T, He K, Garcia V, Bai F, Hao Y, Luan S, Li L (2014) DUF221 proteins are a family of osmosensitive calcium-permeable cation channels conserved across eukaryotes. Cell Res. 24(5):632-5.
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Progress 01/01/13 to 09/30/13
Outputs
Target Audience: Audience: Graduate and undergraduate students. Effort: Students educated through formal classroom instruction, laboratory instruction, development of curriculum or innovative teaching methodologies; internships; workshops; extension and outreach. Faculty member is involved in the formal classroom teaching (Plant Cell Biology for both undergraduate and graduate students), mentoring undergraduate lab training,mentoring high school students through lab training and internship. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? Trained undergraduate students, graduate students and postdoctoral fellows in plant molecular genetics, cell biology, and genomics. How have the results been disseminated to communities of interest? Journal publications and PhD thesis. What do you plan to do during the next reporting period to accomplish the goals? More research will be conducted towards understanding the mechanisms underlying plant responses to stress signals, incluidng cold and drought, high salt, low-nutrient status in the soil, using biochemical, molecular, ell biology, and genomics tools.
Impacts
What was accomplished under these goals?
A number of new findings were reported that provide insights towards better understanding of plant responses to stressful environments.
Publications
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2013
Citation:
Functional analysis of immunophilins in the chloroplasts of Arabidopsis
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
C-terminal processing of reaction center protein D1 is essential for the function and assembly of photosystem II in Arabidopsis.
Che Y, Fu A, Hou X, McDonald K, Buchanan BB, Huang W, Luan S.
Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16247-52
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Auxin controls seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis.
Liu X, Zhang H, Zhao Y, Feng Z, Li Q, Yang HQ, Luan S, Li J, He ZH.
Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15485-90.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Unique features of two potassium channels, OsKAT2 and OsKAT3, expressed in rice guard cells.
Hwang H, Yoon J, Kim HY, Min MK, Kim JA, Choi EH, Lan W, Bae YM, Luan S, Cho H, Kim BG.
PLoS One. 2013 Aug 13;8(8):e72541.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Cyclophilin 20-3 relays a 12-oxo-phytodienoic acid signal during stress responsive regulation of cellular redox homeostasis.
Park SW, Li W, Viehhauser A, He B, Kim S, Nilsson AK, Andersson MX, Kittle JD, Ambavaram MM, Luan S, Esker AR, Tholl D, Cimini D, Ellerstr�m M, Coaker G, Mitchell TK, Pereira A, Dietz KJ, Lawrence CB.
Proc Natl Acad Sci U S A. 2013 Jun 4;110(23):9559-64.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability.
Seok MS, You YN, Park HJ, Lee SS, Aigen F, Luan S, Ahn JC, Cho HS.
Physiol Plant. 2013 Oct 8. doi: 10.1111/ppl.12116.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Cold stress causes rapid but differential changes in properties of plasma membrane H(+)-ATPase of camelina and rapeseed.
Kim HS, Oh JM, Luan S, Carlson JE, Ahn SJ.
J Plant Physiol. 2013 Jun 15;170(9):828-37.
- Type:
Journal Articles
Status:
Published
Year Published:
2012
Citation:
Tonoplast calcium sensors CBL2 and CBL3 control plant growth and ion homeostasis through regulating V-ATPase activity in Arabidopsis.
Tang RJ, Liu H, Yang Y, Yang L, Gao XS, Garcia VJ, Luan S, Zhang HX.
Cell Res. 2012 Dec;22(12):1650-65.
- Type:
Journal Articles
Status:
Published
Year Published:
2012
Citation:
A new wave of hormone research: crosstalk mechanisms.
Cui X, Luan S.
Mol Plant. 2012 Sep;5(5):959-60.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
ABA signaling in guard cells entails a dynamic protein-protein interaction relay from the PYL-RCAR family receptors to ion channels.
Lee SC, Lim CW, Lan W, He K, Luan S.
Mol Plant. 2013 Mar;6(2):528-38.
- Type:
Journal Articles
Status:
Published
Year Published:
2012
Citation:
FERONIA receptor kinase pathway suppresses abscisic acid signaling in Arabidopsis by activating ABI2 phosphatase.
Yu F, Qian L, Nibau C, Duan Q, Kita D, Levasseur K, Li X, Lu C, Li H, Hou C, Li L, Buchanan BB, Chen L, Cheung AY, Li D, Luan S.
Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14693-8.
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Progress 01/01/12 to 12/31/12
Outputs
OUTPUTS: 1. Analysis of immunophilins in the assembly of photosystems. Identified mutants of arabidopsis containing T-DNA insertions in the genes encoding immunophilins located in the chloroplast thylakoid lumen. Studied the phenotypes of mutants in relation to photosynthesis. 2. Study on the calcium signaling network that links stress signals to cellular responses. Identified mutants containing mutations in the genes coding for the calcium sensors (CBLs) and their interacting kinases (CIPKs). Examined the phenotypes of these mutants under different conditions including low-nutrient and high salinity. Made links between the calcium signaling components with plant adaptation to the environmental stress conditions. 3. Identifying a new family of peptide hormones in plants. Studies on a new family of short peptides revealed their important function in plant root development. PARTICIPANTS: Aigen Fu, postdoctoral fellow, Kai He, postdoctoral fellow Wenzhi Lan, postdoctoral fellow Ling Meng, Postdoctoral fellow Renjie Tang, postdoctoral fellow Hou Xin, postdoctoral fellow Veder Garcia, graduate student Thomas Kleist, graduate student Greg Mathews, graduate student TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Findings in our project contributed to the knowledge base of plant stress biology. Such knowledge can be used to breed crops with higher yield under sub-optimal conditions in agriculture. 1. Finding on the immunophilins in the chloroplast has the potential to identify the mechanism of photosynthesis regulation. Higher rate of photosynthesis will be critical for biomass yield in agriculture and bioenergy production. 2. Finding on calcium signaling in low-nutrient adaptation provide means to promote crop production under low soil nutrient condition, meaning less fertilizer use in framing practice and protect environment. 3. Finding on root development regulation by peptide hormones can help understand root development and function and thus provide knowledge for enhancing root physiology and nutrient and water uptake.
Publications
- Meng L, Buchanan BB, Feldman LJ, Luan S. (2012) A Putative Nuclear CLE-Like (CLEL) Peptide Precursor Regulates Root Growth in Arabidopsis. Mol Plant 5, 955-7.
- Vasudevan D, Fu A, Luan S, Swaminathan K. (2012) Crystal structure of Arabidopsis cyclophilin38 reveals a previously uncharacterized immunophilin fold and a possible autoinhibitory mechanism. Plant Cell 24, 2666-74.
- Li S, Yu J, Zhu M, Zhao F, Luan S. (2012) Cadmium impairs ion homeostasis by altering K(+) and Ca(2+) channel activities in rice root hair cells. Plant Cell Environ. May 8. doi: 10.1111/j.1365-3040.2012.02532.x.
- Fu A, Liu H, Yu F, Kambakam S, Luan S, Rodermel S. (2012) Alternative oxidases (AOX1a and AOX2) can functionally substitute for plastid terminal oxidase in Arabidopsis chloroplasts. Plant Cell. 2012 Apr;24(4):1579-95.
- Meng L, Buchanan BB, Feldman LJ, Luan S. (2012) CLE-like (CLEL) peptides control the pattern of root growth and lateral root development in Arabidopsis. Proc Natl Acad Sci U S A. 109, 1760-5.
- Lee SC, Luan S. (2012) ABA signal transduction at the crossroad of biotic and abiotic stress responses. Plant Cell Environ. 35, 53-60.
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Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: Work using genetics, biochemical, cell biology tools has been performed and several findings have been made in the past year. These include finding towards the goal of identifying genes in the stress responses of crop plants, mechanistic analysis of signaling pthways that regulate gene expression and membrane transport activities and photosynthetic processes. These results have been disseminated to the communities of interest through publications in scholarly journals, international and national conferences, and teaching platforms including both classroom and laboratory training sessions. PARTICIPANTS: Individuals who work on this project include several postdoctoral fellows Drs. Aigen Fu, Kai He, Xin Hou and graduate students Veder Garcia and Thomas Kleist. TARGET AUDIENCES: Plant biologists and biologists in general would benefit from reading our findings. Students of minority groups are trained in the laboratory. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
The finding on immunophilins improved our understanding of photosynthesis. The finding on membrane transporters improved our understanding of how plants cope with environmental stress conditions thereby setting up knowledge base for genetic engineering of stress tolerance in crop species.
Publications
- Batistic, O., Kim, K-N., Kleist, T., Kudla, J., Luan, S. (2011) The CBL-CIPK signaling network in plants. Book Chapter in Coding and Decoding of Calcium Signals in Plants. Edited by S. Luan, Springer Publishing Inc.
- Li H, Luan S. (2011) The Cyclophilin AtCYP71 Interacts with CAF-1 and LHP1 and Functions in Multiple Chromatin Remodeling Processes. Mol Plant. 4, 748-58.
- Lan WZ, Lee SC, Che YF, Jiang YQ, Luan S. (2011) Mechanistic analysis of AKT1 regulation by the CBL-CIPK-PP2CA interactions. Mol Plant 4(3):527-36.
- Ahn JC, Kim DW, You YN, Seok MS, Park JM, Hwang H, Kim BG, Luan S, Park HS, Cho HS. (2010) Classification of rice (Oryza sativa L. Japonica nipponbare) immunophilins (FKBPs, CYPs) and expression patterns under waterstress. BMC Plant Biol. 10:253.
- Li H, Luan S. (2010) P53 is a histone chaperone required for repression of ribosomal RNA gene expression in Arabidopsis. Cell Res. 20, 357-66.
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Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: The project focuses on the understanding of molecular mechanism underlying plant response to environmental stress conditions. IN the past year, we have been working on the molecular characterization of plant kinases and phosphatases that are involved in signaling environmental changes. Furthermore, we worked on the immunophilins that function in photosynthesis under various conditions. SOme papers are published and some materials are distributed to the community of plant biology. PARTICIPANTS: Sheng Luan: PI Wenzhi Lan: postdoc Sung Chul Lee: postdoc Lee Chae: Graduate student TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
In the past year, we have identified a calcium channel that is part of the salt-resistant family transporters that usually transport sodium and potassium. This finding will be useful in engineering salt tolerance in plants. We also identified important signaling pathways in response to drought and plant hormone abscisic acid. This information will be helpful in engineering drought tolerance in crops.
Publications
- Chae L, Sudat S, Dudoit S, Zhu T, Luan S. (2009) Diverse Transcriptional Programs Associated with Environmental Stress and Hormones in the Arabidopsis Receptor-Like Kinase Gene Family. Mol Plant. 2, 84-107.
- Sun SY, Chao DY, Li XM, Shi M, Gao JP, Zhu MZ, Yang HQ, Luan S, Lin HX (2009) OsHAL3 mediates a new pathway in the light-regulated growth of rice. Nat Cell Biol. 11, 845-51.
- Chen J, Li LG, Liu ZH, Yuan YJ, Guo LL, Mao DD, Tian LF, Chen LB, Luan S, Li DP. (2009) Magnesium transporter AtMGT9 is essential for pollen development in Arabidopsis. Cell Res. 19, 887-898.
- Lan, W., Lee, S., Buchanan, BB, and Luan, S. (2010) Protein kinase and phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Proc. Natl. Acad. Sci. USA. (in press)
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Progress 10/01/05 to 09/30/10
Outputs
OUTPUTS: Our lab has been working on the plant responses to environmental stress conditions. The project produced a number of findings that are significant in agriculture. These include discoveries in signaling mechanisms used to adapt to the stress conditions, immunophilins involved in photosynthesis, and ion channels that play roles in drought tolerance. PARTICIPANTS: Postdoctoral fellows: Aigen Fu, Wenzhi Lan, Sung Chul Lee Graduate students: Lee Chae TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Important procedures developed during this period include patch-clamping techniques to monitor ion channel activities, protein native gels to measure photosynthetic complexes, and genetics analysis using inducible RNAi, all of which have contributed significantly to the discoveries list below.
Publications
- Chae L, Sudat S, Dudoit S, Zhu T, Luan S. (2009) Diverse Transcriptional Programs Associated with Environmental Stress and Hormones in the Arabidopsis Receptor-Like Kinase Gene Family. Mol Plant. 2, 84-107.
- Sun SY, Chao DY, Li XM, Shi M, Gao JP, Zhu MZ, Yang HQ, Luan S, Lin HX (2009) OsHAL3 mediates a new pathway in the light-regulated growth of rice. Nat Cell Biol. 11, 845-51.
- Chen J, Li LG, Liu ZH, Yuan YJ, Guo LL, Mao DD, Tian LF, Chen LB, Luan S, Li DP. (2009) Magnesium transporter AtMGT9 is essential for pollen development in Arabidopsis. Cell Res. 19, 887-898.
- Lan, W., Lee, S., Buchanan, BB, and Luan, S. (2009) Protein kinase and phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Proc. Natl. Acad. Sci. USA. 106(50):21419-24
- Cheong YH, Sung SJ, Kim BG, Pandey GK, Cho JS, Kim KN, Luan S. (2010) Constitutive overexpression of the calcium sensor CBL5 confers osmotic or drought stress tolerance in Arabidopsis. Mol Cells 29(2):159-65.
- Lan WZ, Wang W, Wang SM, Li LG, Buchanan BB, Lin HX, Gao JP, Luan S. (2010) A rice high-affinity potassium transporter (HKT) conceals a calcium-permeable cation channel. Proc. Natl. Acad. Sci. USA. 107(15):7089-94.
- Li H, Luan S (2010) AtFKBP53 is a histone chaperone required for repression of ribosomal RNA gene expression in Arabidopsis. Cell Res. 20(3):357-66.
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Progress 01/01/08 to 12/31/08
Outputs
OUTPUTS: In the past year, our group has made significant progress towards the understanding of molecular mechanisms underlying plant responses to stressful conditions. These include the following: 1. Identified a family of sodium transporters in rice and characterized their transport functions. 2. Characterized several mutants of immunophilins that are import for plant photosynthesis under various conditions. 3. Identified a number of mutants of CBLs and CIPKs that function in plant responses to low nutrition status. PARTICIPANTS: Wenzhi Lan (postdoctoral fellow working on salt stress tolerance transporters) Aigen Fu (postdoctoral fellow working on immunophilins) Sung Chul Lee (postdoctoral fellow working on CBL-CIPK) Veder Garcia (graduate student working on immunophilins) Amparo Lima (graduate student working on immunophilins) TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Understanding stress response in plants is critical for engineering plants with important agricultural traits. In particular, as the global warming becomes a serious problem, our studies on plant response to high and low temperature, and to drought, significantly increase our measures to cope with climate changes in agriculture.
Publications
- Chen X, Lin WH, Wang Y, Luan S, Xue HW. (2008) An inositol polyphosphate 5-phosphatase functions in PHOTOTROPIN1 signaling in Arabidopis by altering cytosolic Ca2+. Plant Cell 20(2):353-66. Epub 2008 Feb 5. Abstract
- Le-Gong Li, Lubomir N. Sokolov, Yong-Hua Yang, Dong-Ping Li, Julie Ting, Girdhar K. Pandy, and Sheng Luan (2008) A Mitochondrial Magnesium Transporter Functions in Arabidopsis Pollen Development. Mol Plant 1: 675 - 685.
- Girdhar K. Pandey, John J. Grant, Yong Hwa Cheong, Beom-Gi Kim, Le Gong Li, and Sheng Luan (2008) Calcineurin-B-Like Protein CBL9 Interacts with Target Kinase CIPK3 in the Regulation of ABA Response in Seed Germination. Mol Plant 1: 238 - 248.
- Dominguez-Solis JR, He Z, Lima A, Ting J, Buchanan BB, Luan S. (2008) A cyclophilin links redox and light signals to cysteine biosynthesis and stress responses in chloroplasts. Proc Natl Acad Sci U S A. 105(42):16386-91. Epub 2008 Oct 9. Abstract
- Li L, Liu K, Hu Y, Li D, Luan S. (2008) Single mutations convert an outward K+ channel into an inward K+ channel. Proc Natl Acad Sci U S A. 105(8):2871-6. Epub 2008 Feb 19. Abstract
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Progress 01/01/07 to 12/31/07
Outputs
OUTPUTS: The activities on the project include the following components: 1. Characterization of the immunophilins in thte process of photosynthetic biogenesis 2. The molecular connection of CBL-CIPK network with the stress signaling processes such as responses to low temperature, drought, and low-nutrition in the soil. 3. salt transport process and its relevance to the mechanism of salt tolerance in crops. 4. Starch metabolism and its regulation in model plants and its relevance to the crop productivity in the field.
PARTICIPANTS: Sung-Chul Lee, Legong Li, Dale Aromdee, Veder Garcia, Hong Li, Wenzhi Lan, Aigen Fu
TARGET AUDIENCES: Same as before
PROJECT MODIFICATIONS: None
Impacts
We have achieved a number of goals that have important impacts: 1. Finding immunophilins and their functions in the assembly process of photosystem II, the molecular machine that split water into oxygen. 2. FInding a role and mechanism of calcium-dependent signaling pathway in the process of low-potassium sensing and tolerance 3. Finding unique function of HKT-type transporters in rice during salt tolerance and nutrition process 4. Findingmore proteins associated with the starch granule during light-dark cycle and their role in controling the level of starch accumulation in the plant leaves.
Publications
- Li, H., He, Z., Lu, G., Alonso, J., Ecker, J., and Luan, S. (2007) A WD-40 domain cyclophilin interacts with histone H3 and regulates organogenesis and gene silencing in Arabidopsis. Plant Cell 19, 2403-2416.
- Lee, S., Lan, W., Kim, B., Li, L., Buchanan, B.B., and Luan, S. (2007) A protein phosphorylation and dephosphorylation network regulates a plant potassium channel. Proc. Natl. Acad. Sci. USA. 104, 15959-15964.
- Pandey GK, Cheong YH, Kim BG, Grant JJ, Li L, Luan, S. (2007) CIPK9: a calcium sensor-interacting protein kinase required for low-potassium tolerance in Arabidopsis. Cell Res. 17, 411-421.
- Cheong YH, Pandey GK, Grant JJ, Batistic O, Li L, Kim BG, Lee SC, Kudla J, Luan S. (2007) Two calcineurin B-like calcium sensors, interacting with protein kinase CIPK23, regulate leaf transpiration and root potassium uptake in Arabidopsis. Plant J. 52, 223-239.
- Kim BG, Waadt R, Cheong YH, Pandey GK, Dominguez-Solis JR, Schultke S, Lee SC, Kudla J, Luan S. (2007) The calcium sensor CBL10 mediates salt tolerance by regulating ion homeostasis in Arabidopsis. Plant J. 52:473-84. Epub 2007 Sep 6.
- Pandey GK, Cheong YH, Li L, Luan, S. (2008) The calcineurin B-like protein CBL9 and its interacting kinase CIPK3 functions in ABA-regulated seed germination. Mol. Plant 2008 Feb 9; [Epub ahead of print] Chen, X., Lin, W., Luan, S., and Xue, H. (2008) An Inositol polyphosphate 5-phosphatase functions in phototropin 1 signaling through altering cytosolic Ca2+ Plant Cell 2008 Feb 5; [Epub ahead of print]
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Progress 01/01/06 to 12/31/06
Outputs
In the past year, we have made very important findings on the function of immunophilins in the photosynthetic systems. In particular, a FKBP-type immunophilin has been shown to play a critical role in the biogenesis and stability of the photosystem II complex. This finding potentially contribute to the genetic engineering of the crops with higher light-harvesting capacity and thereby increasing yields.
Impacts
Photosystem II holds the key to photosynthetic capacity because it is the largest complex liable to light damage. Our study may provide ways to modify the structural stability of this complex and icnerase the yield of crop plants.
Publications
- Gopalan G, He Z, Battaile KP, Luan S, Swaminathan K. (2006) Structural comparison of oxidized and reduced FKBP13 from Arabidopsis thaliana. Proteins. 65(4):789-95.
- Lima A, Lima S, Wong JH, Phillips RS, Buchanan BB, Luan S. (2006) A redox-active FKBP-type immunophilin functions in accumulation of the photosystem II supercomplex in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 103(33):12631-6. Epub 2006 Aug 7.
- Vasudevan D, Gopalan G, He Z, Luan S, Swaminathan K. (2005) Expression, purification, crystallization and preliminary X-ray diffraction analysis of Arabidopsis thaliana cyclophilin 38 (AtCyp38). Acta Crystallograph Sect F Struct Biol Cryst Commun. 61(Pt 12):1087-9. Epub 2005 Nov 24.
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Progress 01/01/05 to 12/31/05
Outputs
Out laboratory has been working on the isolation of genes related to plant stress responses. Our ecent work identified several genes involved in response to salt and drought conditions. In some cases, these genes may function in nutrition sensing in plants, providing information vital for development of crops with higher tolerance to low nutrient soils. Some publications are listed below.
Impacts
The research results in our lab have impact on molecular breeding of crops with higher tolerance to low water availability, high salt content, and low nutrient status of soils.
Publications
- Liu, K., Li, L., and Luan, S. 2005. An essential function of phosphatidylinositol phosphates in activation of plant shaker-type K channels. Plant J. 42, 433-43.
- Liu, K., Luan, S. 2005. Intracellular potassium sensing in the plant shaker family K-channels. Plant J. (In press)
- Romano, P., Gray, J., Horton, P., and Luan, S. 2005. Plant immunophilins: functional versatility beyond protein maturation. New Phytol. (Tanksley Review) 166(3):753-769.
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Progress 01/01/04 to 12/31/04
Outputs
In the report period, we have further investigated the regulatory mechanism for a voltage-gated potassium channel; conducted genetic analysis of a calcium sensor and its interacting kinases in stress responses and hormone actions; performed structural analysis of a chloroplast immunophilin.
Impacts
A patent "Use of a calcium sensor overexpression to improve crop tolerance to stress conditions" has been filed.
Publications
- Pandey, G., Cheong, Y., Kim, N., Luan, S. (2004) Calcium sensor calcineurin B-like 9 modulates sensitivityand biosynthesis of ABA in Arabidopsis. Plant Cell 16, 1912-1924.
- He, Z. and Luan, S. (2004) Immunophilins and parvulins: superfamily of peptidyl prolyl isomerases in Arabidopsis. Plant Physiol. 134, 1248-1267.
- Gopalan G, He Z, Balmer Y, Romano P, Gupta R, Heroux A, Buchanan BB, Swaminathan K, Luan S. (2004) Structural analysis uncovers a role for redox in regulating FKBP13, an immunophilin of the chloroplast thylakoid lumen. Proc Natl Acad Sci U S A. 101, 13945-50.
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Progress 01/01/03 to 12/31/03
Outputs
In the report period, we have made significant progress on the project. This includes identification of a regulatory mechanism for a voltage-gated potassium channel, genetic analysis of a calcium sensor in a stress process, and genomics analysis of the immunophilin superfamily.
Impacts
Some results are being considered for patent application.
Publications
- Kim, K., Cheong, Y., Grant, J., Pandey, G., and Luan, S. (2003) CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis. Plant Cell 15, 411-423.
- Gupta, R., and Luan, S. (2003) Redox regulation of protein tyrosine phosphatases and MAP kinases in higher plants. Plant Physiol. 132, 1149-1152.
- Hrabak, E., Chan, C., Gribskov, M., Harper, J.F., Choi, J., Halford, N., Kudla, J., Luan, S., et al., and Harmon, A.C. (2003) The Arabidopsis CDPK-SnRK Superfamily of Protein Kinases. Plant Physiol. 132: 666-680.
- Cheong, Y., Kim, K., Pandey, G.K., Gupta, R., Grant, J., and Luan, S. (2003) CBL1, a Calcium Sensor That Differentially Regulates Salt, Drought, and Cold Responses in Arabidopsis. Plant Cell 15, 1833-1845.
- Gupta, R., and Luan, S. (2003) Redox regulation of protein tyrosine phosphatases and MAP kinases in higher plants. Plant Physiol. 132, 1149-1152.
- Hrabak, E., Chan, C., Gribskov, M., Harper, J.F., Choi, J., Halford, N., Kudla, J., Luan, S., et al., and Harmon, A.C. (2003) The Arabidopsis CDPK-SnRK Superfamily of Protein Kinases. Plant Physiol. 132: 666-680.
- He, Z. and Luan, S. (2004) Immunophilins and parvulins: superfamily of peptidyl prolyl isomerases in Arabidopsis. Plant Physiol. (in press).
- Luan, S. (2003) Protein phosphatases in plants. Annu. Rev. Plant Biol. 54, 63-91.
- Romano, P., He, Z., and Luan, S. (2004) Introducing immunophilins: from organ transplantation to plant biology. Plant Physiol. (in press).
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Progress 01/01/02 to 12/31/02
Outputs
This project has made significant progress in the past year. Several important areas of research have been expanded into productive activities. These include genetic analysis of calcium signal transduction and its role in stress tolerance, further study of immunophilins that function in photosynthesis and plant development, and identification of the detoxification transporters.
Impacts
Several areas of agriculturally important research have been expanded. These activities will have impact on engineering stress tolerance of crop plants, photosynthesis and crop productivity, and environmental remediation.
Publications
- Kim, K., Cheong, Y., Grant, J., Pandey, G. and Luan, S. (2003) CIPK3, a calcium-sensor associated protein kinase that regulates abscisic acid and cold signal transduction in arabidopsis. Plant Cell (in press)
- Gupta, R., Mould, R., He, Z. and Luan, S. (2002) A chloroplast FKBP interacts with and affects the accumulation of rieske subunit of cytochrome b/f complex in photosynthetic electron transport. PNAS 99, 15806-15811.
- Gupta, R., He, Z. and Luan, S. (2002) Functional relationship of cytochrome c6 and plastocyanin in arabidopsis. Nature 417, 567-571.
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Progress 01/01/01 to 12/31/01
Outputs
This project is to understand the molecular mechanism of plant tolerance to stress conditions. Several lines of studies are being conducted. 1. Calcium function in stress tolerance: studies focus on functional analyses of a calcium sensor and its target protein kinases in higher plants. 2. Tyrosine phosphorylation and stress tolerance: tyrosine phosphatases are critical components in stress signal transduction. A reverse genetics approach is being used to study the function of genes encoding these phosphatases. 3. Immunophilins in chloroplast: immunophilins are responsive to many stress conditions. They may function to protect plants from being damaged by stress conditions. Genetic tools are being implemented in this study. 4. Identification of membrane transporters and detoxification carriers: many membrane proteins are involved in the detoxification of heavy metals and other toxic materials. A molecular approach is being used to identify the genes encoding these
transporters. Several findings have been resulted from the study in the past year. They are: 1. Identification of a novel transporter for organics and heavy metal detoxification. 2. Finding a mechanism for heavy metal block of a potassium channel. 3. Cloning the first family of magnesium transporters from any multicellular organism.
Impacts
Study of plant response to environmental stress will provide important platforms for engineering crop plants with better tolerance to extreme growth conditions.
Publications
- Li, L., He, Z., Pandey, G., and Luan, S. (2002) Functional cloning and characterization of an Arabidopsis efflux carrier for multidrug and heavy metal detoxification. J. Biol. Chem. (in press)
- Li, L., Gardner, R., and Luan, S. (2001) A novel family of magnesium transport genes in higher plants. Plant Cell 13, 2761-2775.
- Liu, K., and Luan, S. (2001) Internal aluminum block of plant inward K+ channels. Plant Cell 13: 1453-1466.
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Progress 01/01/00 to 12/31/00
Outputs
This project is to understand the molecular mechanism of plant tolerance to stress conditions. Several lines of studies will be conducted. 1. Calcium function in stress tolerance: studies focus on functional analyses of a calcium sensor and its target protein kinases in higher plants. 2. Tyrosine phosphorylation and stress tolerance: tyrosine phosphatases are critical components in stress signal transduction. A reverse genetics approach is being used to study the function of genes encoding these phosphatases. 3. Immunophilins in chloroplast: immunophilins are responsive to many stress conditions. They may function to protect plants from being damaged by stress conditions. Genetic tools are being implemented in this study. 4. Identification of membrane transporters and detoxification carriers: many membrane proteins are involved in the detoxification of heavy metals and other toxic materials. A molecular approach is being used to identify the genes encoding these
transporters.
Impacts
Understanding the mechanism of stress tolerance in higher plants will provide information for development of stress-tolerant plants.
Publications
- Kim, K-N, Cheong, Y., Gupta, R., and Luan, S. (2000) Interaction specificity between calcineurin B-like calcium sensors and their target kinases. Plant Physiol. 124, 1844-1853.
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