Source: OKLAHOMA STATE UNIVERSITY submitted to NRP
MEAT ANALOGS MADE FROM TEXTURIZED VEGETABLE MATERIALS.
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0186808
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2000
Project End Date
Jun 30, 2006
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
OKLAHOMA STATE UNIVERSITY
(N/A)
STILLWATER,OK 74078
Performing Department
HUMAN ENVIRONMENTAL SCIENCES
Non Technical Summary
(N/A)
Animal Health Component
60%
Research Effort Categories
Basic
20%
Applied
60%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
5011549100010%
5011820100010%
5011830100010%
5021549100010%
5021820100016%
5021830100014%
5011830202010%
5021820309010%
5011549202010%
Goals / Objectives
The general aim of the proposed research is to develop meat analogs using texturized soy, wheat and peanut materials to provide consumers with alternative protein sources from Oklahoma-grown crops. Based on consumer evaluation of current commercial meat analogs, meat analogs made from soy and wheat will be developed and evaluated. Ground meal from peanut press-cakes will be thermoplastically-extruded to establish appropriate extrusion parameters to produce texturized peanut extrudates. Meat analogs containing blends of texturized soy, wheat and peanut will be developed and evaluated. The specific objectives are: Phase A: Soy and wheat A1) To evaluate physical and sensory properties of current commercially-manufactured soy-based meat analogs. A2) To evaluate a) functional properties, and b) physical properties of commercial textured low-fat soy and wheat materials. A3) To establish processing protocols to develop meat analogs using texturized low-fat soy and wheat materials as the major ingredients. A4) To evaluate the effects of various formulations and processing protocols on physical properties of meat analogs, and to select prototypes for further studies. A5) To conduct shelf-life studies to evaluate biochemical and physical properties of the selected prototypes. A6) To carry out an optimization study including sensory evaluation to select at least one meat analog with potential for commercialization. Phase B: Soy, wheat and peanut B1) To evaluate structure and biochemical composition of peanut press-cakes produced by mechanical pressing and solvent extraction of oil from seeds. B2) To investigate and establish appropriate processing parameters to obtain thermoplastically-extruded peanut materials from ground press-cake meal. B3) To evaluate physico-chemical and functional properties of thermoplastically-extruded materials from ground peanut press-cake meal, and, using findings from Objective A2, select texturized materials with potential properties for meat analogs. B4) To evaluate the effects of formulations containing various blends of texturized vegetable materials (viz., soy, wheat, peanut) and processing protocols on physical properties of meat analogs, and to select prototypes for further studies. B5) To conduct shelf-life studies to evaluate biochemical, physical, and microbiological properties of the selected prototypes. B6) Based on results from B4 and B5, to carry out optimization studies including sensory evaluation to identify prototypes with potential for commercialization.
Project Methods
Phase A: years 1 - 3. Objective A1: A 4 x 2 factorial of 4 meat analog types and 2 storage periods will be used. Affective sensory tests (to identify which properties of the commercial meat analogs are acceptable/unacceptable), and physical tests (color (Minolta Chroma Meter), texture(TA-XT2 Texture Analyzer), moisture content (AOAC method), and water activity [Rotronic meter]) will be carried out. Objective A2: Response Surface Methodology (RSM) will be used to evaluate the effects of hydration time and temperature, cooking time and temperature on functional properties (e.g., water absorption capacity (WAC), protein dispersibility index (PDI, AACC method), and physical properties (e.g., texture profile, color, pH, aw) of commercial texturized soy and wheat materials. Objectives A3 and A4: Information from Objectives A1and A2 will be used to decide on the types of meat analogs to be developed. RSM will be used to evaluate the effects of processing parameters and major ingredients (soy, wheat) on physical properties (e.g., color, texture, aw, pH) of meat analogs, and to select prototypes for further studies. Objective A5: A factorial arrangement of prototype x storage period will be used for shelf-life studies on the prototypes selected from Objective A4. Biochemical (fatty acid profiles [GC, AOCS method], GC-headspace aldehydes), microbial (aerobic mesophiles and psychrotrophs, E. coli), and physical tests will be carried out. Objective A6: RSM will be utilized to optimize development of at least one soy-wheat based meat analog with good physical and biochemical quality, and sensory attributes that are not inferior to current commercial meat analogs. Phase B: Years 2 - 5 Objective. B1: The effect of oil extraction protocols on biochemical composition (crude protein and lipid [AOAC], fatty acid profiles, PDI), texture, and micro-structure (using scanning electron microscopy), of peanut press-cakes will be evaluated by a factorial design. Objective B2: RSM will be used to establish appropriate thermoplastic-extrusion parameters (e.g., feed moisture content, barrel temperature) and press-cake production protocols to obtain texturized peanut materials using a twin-screw extruder. Objective B3: RSM will be used to identify texturized peanut materials which show potential biochemical, physical and functional properties for making meat analogs. Objective B4: Findings from objectives B3, and A3-A6 will determine the type of meat analogs to develop. RSM will be used to evaluate the effects of formulation (soy/wheat/peanut) and processing protocol on physical properties of the analogs. Objective B5: Shelf-life studies (prototype x storage period) will be carried out on analog prototypes selected from objective B4. Biochemical, physical, and microbial properties of the prototypes will be evaluated. Objective B6: An optimization study (RSM ) will be carried out to identify soy/peanut/wheat meat analogs with potential for commercialization. Proximate composition and fatty acid profiles of the selected analogs will be determined. All data collected will be analyzed using the SAS computer package (PC-SAS version 7).

Progress 10/01/00 to 06/30/06

Outputs
The study investigated preparation and use of textured peanut (TP), prepared by extrusion processing (Wenger TX-52 twin screw extruder) of peanut meal (~10%fat) from partially-defatted raw peanuts. Physical and functional properties, lipid profile and volatile flavor compounds of TP were evaluated using instrumental methods. From those results, optimization of physical properties of the TP with polysaccharide binders, and evaluation of commercial beef and soy products, two applications for TP were investigated. Hamburger-style patties containing 0-80% re-hydrated TP were prepared and evaluated by instrumental and sensory tests. Re-hydrated TP was mixed with binders (1.25% Carrabind 80A, 1.35% Colloid Bind I-96), dried plum, spices, chicken flavor, formed into nugget-style analogs and cooked, then coated (commercial coatings), baked, and evaluated. The dry TP was light brown in color (hue angle = 73, chroma =16, L value = 36), with moisture (%) and water activity ranges of 5.8-7.9 and 0.44-0.61, respectively. It contained 7.8% fat, of which 73.7-74.9%, 16.8-17.4%, and 7.0-8.7% were monounsaturated, saturated, and polyunsaturated fat, respectively. Of the 20 volatile flavor compounds identified in the TP, there were nine lipid oxidation products and six pyrazines present at subthreshold levels indicating that the TP would not impart beany or roasted peanut flavors, respectively. N-methylpyrolle, the most abundant compound (2-3 ppm) in the TP, has no established threshold, and might be responsible for the slight 'grape-nut cereal' flavor of the bland TP. Hamburger-style patties containing 0-60% TP were similar in hardness, but were harder and deeper brown than those with 80% TP. Patties containing 20-80% TP were not significantly different in water activity, springiness, and adhesiveness from lean (10% fat) beef patties. Cook-yield, moisture, and cohesiveness of the patties increased significantly (p<0.05) as TP content was increased. The monounsaturated to saturated fat ratio (MUFA:SAT) of the patties increased with increased TP, such that MUFA:SAT were 1:1 and 2:1 for beef and 80%TP patties, respectively. Patties containing 0-40% TP were acceptable in appearance, smell, taste, and texture to sensory panelists. Hardness and chewiness of the TP nugget-style analogs increased with increased plum and Kraft coating (KC), whereas moisture increased with increased KC. When chicken flavor was 2-3%, springiness of the analogs increased with increased KC; but at 4% chicken flavor, springiness increased as coating was increased and plum was decreased. The most acceptable nugget analogs contained 0-0.2% plum and 2.5-3.5% chicken flavor, and had 33-40% KC in the coating mix. There is potential for value-added use of TP as a meat extender and meat analog. Patties with 20-40%TP (5% fat) would be acceptable alternatives to lean or regular beef patties. Acceptability of 80% TP patties would be improved by adding brown seasoning and binders. The acceptable TP chicken-nugget analogs have potential to reduce cardio vascular disease risk due to their lower (2%) fat content and higher MUFA:SAT (2:1) compared to commercial chicken nuggets (~15%fat, 1:1 MUFA:SAT).

Impacts
Textured peanut (TP), a low-fat product having physical properties similar to ground beef but with a bland flavor, was prepared from partially-defatted, ground peanuts. Hamburger-style patties containing up to 40% TP, and meat-less chicken-nugget style analogs made from TP and chicken flavor had properties similar to traditional beef and chicken nuggets, respectively, and were acceptable to consumer panels. The TP patties and nugget-analogs have potential to reduce cardio vascular disease risk due to the quantity and type of fat that they contain. This production of acceptable products from textured peanut indicates potential for value-added use of defatted peanuts (as well as the peanut press-cake formed during peanut oil manufacture) for manufacture of meat extenders and meat analogs.

Publications

  • Hinds MJ, Riaz MN, Moe D, and Scott DD. Fatty Acid and Volatile Flavor Profiles of Textured Partially-defatted Peanut. 2005. In Food Lipids - Chemistry, Flavor, and Texture; Shahidi F, Weenen H (Eds.). American Chemical Society, Washington, DC. Chapter 16: 205-219.
  • Hinds MJ, Riaz MN, Moe D, and Scott DD. Optimization of Physical Properties of Textured Peanut Patties using Binders. 2005. Proceedings of the 37th Annual Meeting of the American Peanut Research and Education Society; Sholar JR (Ed.). 37: 41.
  • Hinds MJ, Riaz MN, Moe D, Scott DD, Salam A. 2003. Lipid profile of beef patties containing texturized peanut. Book of Abstracts: 2003 Annual Meeting of the Institute of Food Technologists, pp 60-61. IFT. Chicago, IL.
  • Hinds MJ, Riaz MN, Moe D, Scott DD. 2003. Physical properties of beef patties extended with texturized peanut protein. Book of Abstracts: 2003 Annual Meeting of the Institute of Food Technologists, pp 216. IFT. Chicago, IL.
  • Hinds MJ, Riaz MN, Moe D, Scott DD. 2002. GC-MS Analysis of Volatile Flavor Compounds Arising from Twin-screw Extrusion Processing of Peanuts. Proceedings of the 34th Annual Meeting of the American Peanut Research and Education Society. 2002; 34: 109-110.
  • Hardy, DA. 2003. Physical and sensory properties of a calcium-fortified pasta made with defatted peanut flour. MS thesis, Oklahoma State University, Stillwater, OK.


Progress 10/01/04 to 09/30/05

Outputs
The major aim is the development of meat analogs that may reduce the risk of cardiovascular disease. Physical tests and biochemical analyses have been completed on textured peanut (TP) prepared by extrusion processing of partially-defatted peanut meal. The TP has meat-like properties, but lacks compounds that may impart off-flavors. Combination TP-beef patties, and 100% TP patties with good physical properties have been made. Patties containing 100% TP have been flavored with herbs and spices; and some with commercial chicken flavor to mimic 'chicken-nuggets'. A secondary objective of this project is value-added utilization of defatted peanut meal.

Impacts
The meat analogs containing textured peanut, and the peanut-based muffins and pasta can provide consumers with more healthy alternatives to traditional food products.

Publications

  • Hinds MJ. Physical properties of muffins containing peanut flour and peanut butter. Peanut Science. 2003; 30(1): 68-74. [published in May 2005 due to unexpected death of former publisher]


Progress 10/01/03 to 09/30/04

Outputs
Hamburger-style patties containing 20-80% rehydrated texturized peanut (TP) replacing beef have been developed, and their physicochemical properties have been evaluated. Patties containing 20-80% TP are similar in texture profile (hardness, cohesiveness, chewiness, springiness) to 100% beef patties, but contain significantly more moisture. As beef is replaced by TP, total fat content of the patties decrease, but the monounsaturated and polyunsaturated fatty acid levels increase. Patties containing 80% TP have a 2:1 monounaturated:saturated fatty acid ratio. Potential value-added products containing 100% TP are being investigated.

Impacts
Texturized peanut, when used as a beef extender, has the potential to lower the fat content while raising the ratios of monounsaturated and polyunsaturated fatty acids, and this has positive cardiovascular implications.

Publications

  • No publications reported this period


Progress 10/01/02 to 09/30/03

Outputs
The aim of this project is the development of meat analogs that may reduce the risk of cardiovascular disease. Physical tests (including texture profile) and chemical analyses (fat and flavor profiles using GC-MS) have been carried out on texturized peanut prepared by extrusion processing of partially-defatted peanut meal. The texturized peanut has meat-like properties, but lacks compounds that may impart off-flavors. Potential commercial applications for it are being investigated.

Impacts
Textured peanut contains 8% total fat with a 17:83 ratio of saturated to unsaturated fat making it cardiovascular friendly. Even after one-year refrigerated storage, hexanal, the main component of the 'beany' flavor (associated with textured soy) is present at only 0.2ug/g in the textured peanut compared with the level of 5ug/g which is necessary to perceive a 'beany' flavor.

Publications

  • Hinds, M.J., Jolly, C.M., Nelson, R.G., Donis, Y., and Prophete, E. 2003. Comparative study of acceptability and properties of Haitian and US honey-roasted peanuts. International Journal of Consumer Studies. 27(2):157-167.
  • Hinds, M.J., Jolly, C.M., Nelson, R.G., Donis, Y., and Prophete, E. 2002. Consumer acceptability and physicochemical properties of Haitian peanut butter-type products (mambas) compared with US peanut buter. Peanut Science. 29(2): 102-109.


Progress 10/01/01 to 09/30/02

Outputs
The aim of this project is the development of meat analogs that may reduce the risk of cardiovascular disease. Physical tests (including texture profile) and chemical analyses (fat and flavor profiles using GC-MS) have been carried out on texturized peanut prepared by extrusion processing of partially-defatted peanut meal. The texturized peanut has meat-like properties, but lacks compounds that may impart off-flavors. Potential commercial applications for it are being investigated.

Impacts
The texturized peanut has potential for use as a meat extender and meat analog without imparting the `beany' flavor typical of other texturized vegetable proteins. These findings indicate that new value-added products could be manufactured from currently underutilized defatted peanut meal.

Publications

  • GC-MS Analysis of Volatile Flavor Compounds Arising from Twin-screw Extrusion Processing of Peanuts. M.J. Hinds, M.N. Riaz, D. Moe and D. Scott. Paper No. 109. Presented at the 34th Annual Meeting of the American Peanut Research and Education Society, July 14-19, 2002, Raleigh, NC.
  • Characterization of texturized peanut material processed by twin-screw extrusion. M.J. Hinds, M.N. Riaz, D. Moe, D. Scott and A. Salam. 2002. Paper No. P-13. 2nd Annual Food Science Research Symposium, The Oklahoma Food and Agricultural Products Research and Technology Center, April 11, 2002, Stillwater, OK.


Progress 10/01/00 to 09/30/01

Outputs
Sensory evaluation of five commercial soy-based meat analogs was carried out by 81 panelists randomly recruited from OSU. Using a beef patty as the control, each panelist indicated their acceptability of various attributes of the six products using 5-pt Hedonic scales. Instrumental objective analyses were also carried out. Appropriate ranges for texture and color of meat analogs, the most important attributes influencing acceptability of the tested products, were determined.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 10/01/99 to 09/30/00

Outputs
This project was new October 2000 and there is no progress to report this period.

Impacts
(N/A)

Publications

  • No publications reported this period