Progress 10/01/00 to 09/30/05
Outputs This research program has encompassed a multistage, multiphase series of studies to intensify the production of freshwater prawns. It has incorporated the details of substrate inclusion, including color, materials, mesh sizes,etc as well as other aspects of production all the way from the hatchery, nursery (size grading), to production innovations (polyculture), to feeds and feeding, and live transport technologies. Research was primarily conducted in 0.04 ha experimental ponds that were initially stocked with 45-60 day nursed juveniles (0.25-0.50 g). The growing season was 110-120 days (early June - Mid- September and there were three replications (ponds) for each treatment evaluated. Size grading of juveniles prior to pond stocking had been shown to increase overall production. This management practice appears to separate an established size hierarchy and disrupt the re-establishment of aggressive interactions among individuals that can reduce collective rates of
growth. Grading of juveniles prior to pond stocking significantly increased total production (2,988 kg/ha vs. 2,589 kg/ha) and average harvest weights (52 g vs. 45 g) (Tidwell et al., 2003). The effect of diet changes was another topic of investigation. Prawns are efficient grazers of natural foods and probably derive as much as 1/3 of their nutrition from natural food items, even when prepared diets are fed and the prawn biomass in the ponds is very high. However, some results suggested that at high biomasses, and large individual sizes, high quality, nutrient dense diets might be justified. Data generated here indicate that feeding a high protein penaeid diet throughout the production season was not superior to phase feeding, or the use of a cheaper sinking catfish feed. Just completed studies indicate that neither color, mesh material, nor mesh size of added substrate significantly influenced prawn production in tanks or ponds. The primary factor involved is the amount of substrate
that is added. These finds have made the practical application of substrate technologies much more realistic, lowering material costs from approximately $2,000/ acre to less than $500/acre. When these different technologies are combined, current production rates (2,990 kg/ha) are almost 200 percent greater than the average production rates realized when this research was initiated (1,000 kg/ha). Equally important is that average harvest weight has not decreased at these higher production levels, rather increasing by approximately 45 percent. Substrate technologies appear extremely promising and appear to be additive with other production innovations. These technologies lend themselves to the relatively small pond (0.5 ha) sizes used in the temperate region. Preliminary budgets indicate that the substrate technology is extremely cost effective, especially since less expensive materials have proven to be equally effective.
Impacts In recent years, the commercial production of the freshwater prawn has increased dramatically in the south central United States. This fledgling industry now encompasses over 2,000 acres and is estimated to involve over 500 farm families in 8 states. These studies are designed to provide practical research based information to support this new enterprise. Positive research findings have been field tested under a Yield Verification Program and results have been disseminated to the public via workshops, newsletters and presentations. Recent production improvements of over 200 percent have been achieved by combining individual research improvements into what we call Best Management Practice. Our goal is to translate these production numbers into commmercial scale improvements to ensure economically viable production.
Publications
- Tidwell, J.H. and D.J. Bratvold. 2005. Utility of added substrates in shrimp culture. In: E. Axim, Editor. Periphyton: Ecology, Exploitation, and Management. CAB International Publishing Co., Wallingford, United Kingdom. Pp 247-268.
- Refereed Journal Tidwell, J.H., S.D. Coyle, A. VanArnum, and C. Weibel. 2000. Production response of freshwater prawns Macrobrachium rosenbergii to increasing amounts of artificial substrate in ponds. Journal of the World Aquaculture Society, 31(3)452-458.
- Book Chapters Tidwell, J.H. and L.D. DAbramo. 2000. Grow-out systems - culture in temperate zones. In Freshwater Prawn Farming: The Farming of Macrobrachium rosenbergii (Ed. M.B. New and W.C. Valenti). Blackwell Scientific. Oxford.
- Tidwell, J.H., S.D. Coyle, A.VanArnum, C. Weibel, and S. Harkins. 2000. Growth, survival, and body composition of cage-cultured Nile tilapia, Oreochromis niloticus, fed pelleted and unpelleted distillers grains with solubles in polyculture with freshwater prawn, Macrobrachium rosenbergii. Journal of the World Aquaculture Society, 31(4)627-631.
- Tidwell, J.H., S.D. Coyle, A. VanArnum, C. Weibel, and L. DAbramo. 2001. Use of artificial substrates to maximize production of freshwater prawns in temperate climates. World Aquaculture Vol. 32(3)40-42.
- Tidwell, J.H., S.D. Coyle, A. VanArnum, L.A. Vitatoe, and M. McCathy. 2001. The effect of photoperiod on the nursery production of juvenile freshwater prawn, Macrobrachium rosenbergii. Journal of Applied Aquaculture. 11(4)41-48.
- Tidwell, J.H., S.D. Coyle, A. VanArnum, and C. Weibel. 2002. Effect of substrate amount and orientation on production and population structure of freshwater prawns, Macrobrachium rosenbergii in ponds. Journal of World Aquaculture Society 33,63-69.
- Coyle, S., J.H. Tidwell, A. VanArnum, L.A. Bright. 2003. A comparison of two feeding technologies, in freshwater prawns, Macrobrachium rosenbergii, raised at high biomass densities in temperate ponds. Journal of Applied Aquaculture, 14(1/2)125-135.
- Coyle, S. S. Dasgupta, J.H. Tidwell, A. VanArnum, and L.A. Bright. 2003. Effects of stocking density on nursery production and economics of the freshwater prawn Macrobrachium rosenbergii. Journal of Applied Aquaculture, 14(1/2)137-148.
- Xiong, S., Y.L. Xiong, S.P. Blanchard, B. Wang, and J.H. Tidwell. 2002. Tenderness evaluation of prawns (Machrobrachium rosenbergii) marinated in various salt and acid solutions. International Journal of Food Science Technology 37,291-296.
- Coyle, S.D., J.H. Tidwell, and A. VanArnum. 2001. The effect of biomass density on transport survival of juvenile freshwater prawn, Macrobrachium rosenbergii. Journal of Applied Aquaculture, Vol. 11(3)57-63.
- Dasgupta, S., and J.H. Tidwell. 2003. A breakeven price analysis of four hypothetical freshwater prawn, Macrobrachium rosenbergii, farms using data from Kentucky. Journal of Applied Aquaculture, 14(1/2)1-22.
- Dasgupta, S., and S. Templeton. 2003. Comparing of Kentucky-grown freshwater prawn with marine shrimp & results of a taste test. Journal of the Kentucky Academy of Science, 64(2)128-132.
- Tidwell, J.H., S.D. Coyle, L.A. Bright, A. VanArnum, C. Weibel. 2003. The effects of size grading and length of nursery period on growth and population structure of freshwater prawns stocked in temperate zone ponds with added substrates. Aquaculture. 218, 209-218.
- Coyle, S., J.H. Tidwell, L.A. Bright, and D. Yasharian. 2004. Effect of different feeding strategies on production and economic returns for freshwater prawn, Macrobrachium rosenbergii, raised in earthen ponds in a temperate climate. Journal of Applied Aquaculture, 16(1/2)147-156.
- Tidwell, J.H., S. Coyle, and S. Dasgupta. 2004. Effects of stocking different fractions of size-graded juvenile prawns on production and population structure during a temperature limited growout period. Aquaculture, 231,123-134.
- Coyle, S.D., J. Tidwell, T. Beavers, L.A. Bright, and D. Yasharian. 2005. Comparative efficacy of anesthetics for the freshwater prawn, Macrobrachium rosenbergii. Journal of the World Aquaculture Society, 36(3)282-290.
- Tidwell, J.H., L.R. DAbramo, S.D. Coyle, and D. Yasharian. 2005. Overview of recent research and development in temperate culture of the freshwater prawn (Macrobrachium rosenbergii) in the South Central United States. Aquaculture Research, 36 (3)264-277.
- Yasharian, D.K., S. Coyle, J. Tidwell, and W.E. Stilwell, IV. 2005. The effect of tank coloration on survival, metamorphosis rate, growth, and time to reach the post-larvae stage in freshwater prawn Macrobrachium rosenbergii. Aquaculture Research, 36(3)278-283.
- Extension & Trade Publications Coyle, S.D., J.H. Tidwell, A. VanArnum, and C. Weibel. 2000. The effect of hauling density on survival of juvenile freshwater prawn. Aquaculture Magazine.
- Coyle, S.D., J.H. Tidwell, A. VanArnum, and C. Weibel. 2000. Effect of hauling density on survival of juvenile freshwater prawns. Kentucky Fish Farming Newsletter, 13(1)3.
- Vitatoe, L.A., J.H. Tidwell, S.D. Coyle, and A. VanArnum. 2000. Controlling air breathing insects in freshwater prawn ponds. Aquaculture Magazine. November/December.
- Vitatoe, L., J.H. Tidwell, S.D. Coyle, and A. VanArnum. 2000. Effectiveness of plant and animal oils for control of air breathing insects prior to stocking freshwater prawn ponds. Kentucky Fish Farming Newsletter, 13(1)2-3.
- Tidwell, J.H. 2001. Freshwater prawns. Farm Pond Harvest 35(1) 18-26.
- Coyle, S.D. and J.H. Tidwell. 2002. U.S. research studies feeding technologies for freshwater prawns. The Global Aquaculture Advocate.
- Coyle, S. & J. Tidwell. 2002. Effect of different feeds and feeding technologies on prawn production. Kentucky Aquatic Farming Newsletter, Vol. 15(2). Pg. 3.
- Coyle, S.D. and J.H. Tidwell. 2003. Effect of different feeds and feeding technologies on prawn production. World Aquaculture Magazine, Vol. 34(2)51-53.
- Tidwell, J. and S. Coyle. 2002. Research Update - Prawns. Kentucky Aquatic Farming Newsletter, Vol. 15(4)5. Yasharian, D., B. Durborow, J. Tidwell, and S. Coyle. 2002. Freshwater prawn production in Kentucky: direction for the industry based on 2002 production data. Kentucky Aquatic Farming Newsletter. Vol. 15(4)3.
- Yasharian, D., S. Coyle, J. Tidwell, and C. Boyd. 2002. Gypsum to Control pH in Shrimp Ponds. Kentucky Aquatic Farming Newsletter. Vol. 15(2)2.
- Coyle, S.D. and J.H. Tidwell. 2003. Effect of feeds, feeding and natural foods on freshwater prawn production. pp: 533-539 In T.P. Lyons and K.A. Jacques, Editors. Nutritional Biotechnology in the Feed and Food Industries. Nottingham University Press, Nottingham, United Kingdom.
- Dasgupta, S. and J.H. Tidwell. 2003 Freshwater prawn culture at Kentucky State University, An economic analysis. pp 28-31 In P. Perschbacher, Editor.
- Yasharian, D., B. Durborow, J. Tidwell, and S. Coyle. 2003. Freshwater prawn production in Kentucky. Aquaculture Magazine, 29(5)17-18.
- Yasharian, D., S. Coyle, J. Tidwell, and L.A. Bright. 2004. Evaluation of transport technologies for live hauling adult freshwater prawn Macrobrachium rosenbergii. Kentucky Aquatic Farming 17(1):5-6.
- Yasharian, D., S. Coyle, and J. Tidwell. 2004. Hatchery and nursery production of the freshwater prawn Macrobrachium rosenbergii in temperate climates. Global Aquaculture Advocate. August 2004.
- Coyle, S.D., L.A. Bright, D. Yasharian, and J.H. Tidwell. 2005. A comparison of pond production of juvenile prawns stocked as 30 and 60 day nursed juveniles. Kentucky Aquatic Farming newsletter, spring 2005, pp. 5-6.
- Coyle, S.D., D. Yasharian, J. Danaher, and J.H. Tidwell. 2005. Comparison of polyculture production of freshwater prawn Macrobrachium rosenbergii with Nile tilapia Oreochromis niloticus stocked as either free ranged or confined in cages. Kentucky Aquatic Farming newsletter, spring 2005, pp. 3-4.
- Tidwell, J.H. and S. Coyle. 2005. Freshwater prawn feed management. Global Aquaculture Advocate, Vol. 7(6)58-59.
- Yasharian, D., S.D. Coyle, J. Tidwell, A. Caporelli, N. Skudlarek, and J. Kimpara. 2005. Kentucky prawns bound for the big apple. Kentucky Aquatic Farming newsletter, Spring 2005, pp. 2-3.
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Progress 01/01/04 to 12/31/04
Outputs A previous study demonstrated that the polyculture of tilapia in cages floated in prawn ponds actually increased prawn production 28% and improved water quality. This was due to the tilapia grazing down the phytoplankton populations. Research in 2004 was conducted to evaluate whether the tilapia might have even more impact if they were not confined in cages and were able to graze freely in all areas of the ponds. The experiment was conducted in nine 0.04 hectare ponds and consisted of three treatments with three replicates each. Control ponds contained only prawns. Caged polyculture ponds contained prawns plus two cages of tilapia (4,800 tilapia/ha). The third treatment was polyculture with the same number of tilapia, but not confined in cages. All ponds contained circulators and substrate. All ponds were stocked with top-graded, 60-day nursed juvenile prawn (0.93 g) at 40,000/ha. Polyculture treatments used monosex male tilapia (90 g). Tilapia were fed a 32% protein
floating pellet once a day to satiation while the prawn were fed a 32% protein sinking prawn diet twice daily based on a feed chart. Water quality parameters including total ammonia-N, un-ionized ammonia nitrogen, and nitrite nitrogen were analyzed three times weekly. Dissolved oxygen, temperature, and pH were analyzed twice daily. Plankton samples were taken on a fortnightly basis. Tilapia and prawn were sampled every three weeks to determine growth rates. At harvest there were no significant differences in treatments in terms of water quality. When tilapia were confined in cages they had no significant impact on prawn weight, total production, or survival. However, when the same number of tilapia were stocked unconfined, they had a significant negative impact on prawn weight, production, and survival. There was no significant difference in tilapia growth or survival when cultured in cages or unconfined. Treatment differences are due to the fact that unconfined tilapia were able to
reproduce. Resulting young-of-the-year tilapia were able to compete directly with the freshwater prawns for food, negatively impacting prawn growth and survival. Prawn/tilapia polyculture is a very viable alternative. However, tilapia should be confined in cages to control reproduction and allow ease of harvest.
Impacts In recent years, the commercial production of the freshwater prawn has increased dramatically in the south central United States. This fledgling industry now encompasses over 500 farm families in 8 states. These studies are designed to provide practical research based information to support this new enterprise. High pH levels have been a recurring problem in prawn production ponds. This research demonstrates a biological method for reducing pH levels while producing a second salable crop.
Publications
- Coyle, S., S. Dasgupta, J.H. Tidwell, A. VanArnum, L.A. Bright. 2003. Effects of stocking density on nursery production and economics of the freshwater prawn Macrobrachium rosenbergii. Journal of Applied Aquaculture, 14(1/2):137-148.
- Osunde, I.M., S. Coyle, J. Tidwell. 2003. Acute toxicity of copper to juvenile freshwater, Macrobrachium rosenbergii. Journal of Applied Aquaculture, 14(3/4):71-79.
- Tidwell, J.H., S. Coyle, S. Dasgupta. 2004. Effects of stocking different fractions of size-graded juvenile prawns on production and population structure during a temperature limited growout period. Aquaculture, 231:123-134.
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