Source: OKLAHOMA STATE UNIVERSITY submitted to NRP
CALCIUM AND PHOSPHORUS REQUIREMENTS AND BIOAVAILABILITY IN THE HORSE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0185233
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2000
Project End Date
Sep 30, 2004
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
OKLAHOMA STATE UNIVERSITY
(N/A)
STILLWATER,OK 74078
Performing Department
ANIMAL SCIENCE
Non Technical Summary
Calcium and Phosphorus Requirements for young growing horses. This study will focus on specific mineral requirements of the horse and how these requirements affect growth and development.
Animal Health Component
(N/A)
Research Effort Categories
Basic
100%
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
30238101010100%
Goals / Objectives
1. Determine the dietary Ca and P requirements for maximizing bone growth and mineralization and minimizing developmental orthopedic problems. 2. Quantify the dietary Ca and P requirements of young, growing horses in order to reduce the potential of environmental nutrient pollution. 3. Quantify Ca and P bioavailability to maximize absorption and mineralization and minimize excessive feeding and subsequent excretion of nutrients.
Project Methods
Experiment I Fifteen yearling Quarter Horses will be assigned diets containing 85, 100, and 115 percent of the NRC recommended concentrations of calcium required for yearling horses. Experimental diets will be formed by supplementing the basal diet with limestone to achieve rations that contain 85, 100, 115% of NRC recommendations for calcium. The 25 week trial will consist of three 72 hr fecal and urine collection periods at day 0, 90 and 180. Mineral availability and balance will be determined by difference from intake and excretion data.Weekly growth data will be recorded on hip, hock, knee, shoulder, and wither height, heart girth circumference and body weight. Two radiographs will be taken of the third metacarpal, one anterioposterior and one lateromedial, on day 0, 90 and 180 of the trial. Radiographs will be scanned and relative bone density will be determined using procedures that will be slightly modified from those of Meakim et al. (1981). All radiographs will evaluated by a veterinary clinician for signs of developmental orthopedic disease (osteochondrosis and epiphysitis) Venous blood samples will be analyzed for biochemical markers of bone resorption and formation. Bone-specific alkaline phosphatase (an enzyme that is an excellent indicator of bone formation activity) will be analyzed by competitive enzyme immunoassay procedures (Alkphase-B kits from Metra Biosystems, Mountain View, CA) and has been validated for use in horses (Hoyt and Siciliano, 1999). Experiment II Fifteen weanling Quarter Horses will be used in a split-plot design experiment to evaluate the effects of varying levels of Ca and P on mineral balance, bone metabolism and growth. Horses will be weaned at 4 mo of age, paired by age and sex and then randomly allotted to treatments. Experimental diets will be formulated to contain 75, 100 and 125% of NRC requirements for Ca. The Ca:P ratio will be held constant at 2 to 1 across treatments. Data for determination of weekly growth data, mineral balances, concentrations of biochemical markers, bone density and incidence of developmental orthopedic disease will be taken at 150, 240 and 330 d of age as outlined in the previous yearling study. Experiment III Six mature sedentary geldings will be used in a 6 by 6 Latin Square design experiment to evaluate the effects of phytase (Natuphos, BASF Mount Olive, NJ) on the availability of phosphorus (P). Treatment diets will consist of a basal ration (100% NRC requirement for P), a medium-low P diet (85% NRC requirement for P), a low P diet (75% NRC requirement for P), basal ration plus phytase, medium-low P diet plus phytase and low P diet plus phytase. Total urine and fecal collections will be conducted at the end of each 21 day period and mineral balances and availabilities will be determined by difference. Blood samples will be taken for the quantification of serum P and parathyroid hormone in order to evaluate P status. Data from this study will aid in the determination of mineral requirements for horses and help reduce the incidence of excessive feeding and subsequent excretion of P, thereby decreasing environmental pollution.

Progress 10/01/00 to 09/30/04

Outputs
In experiment 1, sixteen yearling Quarter Horses were used in a split-plot designed experiment to determine the effects of exercise on bone metabolism. Horses were blocked by sex and BW and then randomly assigned to either the exercised (EX) or non-exercised (NEX) treatment. Radiographs of the third metacarpal were taken on d 0, d 45 and d 90 for determination of radiographic bone aluminum equivalence (RBAE). Although no differences between treatments were found in RBAE within days measured, a significant decrease in RBAE was observed between d 0 and 90 for the NEX horses. This decrease in bone optical density throughout the duration of the trial would indicate that stalling and lack of exercise may result in a greater loss of bone mineral content as compared to the horses forced to exercise. In experiment 2, this study examined the effect of phytase supplementation on dry matter and phosphorus digestibility in mature horses. Four mature sedentary Quarter Horse geldings were used in a 4x4 Latin square design experiment. The treatments were as follows: 1) control (0) with no phytase added to the basal ration, 2) 300 FTU/kg added to the basal ration, 3) 600 FTU/kg added to the basal ration, and 4) 900 FTU/kg added to the basal ration. Phosphorus digestibility was not improved with the addition of supplemental phytase (P>.05). In experiment 3, Eight Quarter Horse yearlings were used in a split-plot designed experiment to estimate fecal output (FO) and dry matter digestibility (DMD) using different methods of chromic oxide (Cr2O3) administration. The two methods of Cr2O3 application were: 1) mixed with the concentrate during pelleting (mixed diet) and 2) top-dressed on the concentrate prior to feeding (top-dressed diet). Calculated FO was greater in the mixed diet versus the top-dressed. Consequently, DMD was lesser in the mixed diet versus the top-dressed. However, measured FO did not differ between treatments. Within the mixed diet, calculated FO was greater than the measured FO. It appears from these data that the use of Cr as an external marker may result in an overestimation of fecal output and subsequent underestimation of dry matter digestibility. In experiment 4, fifteen yearling Quarter Horses were used in a split-plot designed experiment evaluate the response of calcium (Ca) metabolism to varying concentrations of Ca in the diet. Diets were formulated to contain Ca levels at 115% (high, H), 100% (basal, B), and 85% (low, L) of NRC requirements. The 25-week trial consisted of three 72-hour collection periods at day 0 (period I, 12 months of age), day 90 (period II, 15 months of age), and day 180 (period III, 18 months of age) during which complete urine and fecal collections were taken. Results from this study demonstrate an increased Ca retention with increased intake during the first two periods. During period III however, horses consuming diets at or below NRC recommendations, as a percentage of the total diet, experienced an increased absorption, retention, and digestibility of Ca during this time of increased growth rate.

Impacts
Data from this trial may better aid in defining the exercise requirements for young, growing horses in order to optimize bone growth and integrity.

Publications

  • J.C. Henry, S.R. Cooper, D.W. Freeman and J.R. Kropp. 2003. Effects of Exercise on Bone Metabolism in Yearling Horses. Prof. Anim. Sci. 19: 435-438.
  • D.P. Patterson, S.R. Cooper, D.W. Freeman, R.G. Teeter. 2002. Effects of phytase supplementation on dry matter and phosphorus digestibility in horses. J. Equine Vet. Sci 22:456-459.
  • D.P. Patterson, S.R. Cooper, D.W. Freeman, R.G. Teeter. 2002. Estimation of fecal output and dry matter digestibility using various chromic oxide marker methods in the horse. Prof. Anim. Sci. 18:176-179.
  • A.D. Moffett, S.R. Cooper, D.W. Freeman and H.T. Purvis. 2002. Response of yearling quarter horses to varying concentrations of dietary calcium. Prof. Anim. Sci. 18:277-281.


Progress 10/01/02 to 09/30/03

Outputs
Sixteen yearling Quarter Horses were used in a split-plot designed experiment to determine the effects of exercise on bone metabolism. Horses were blocked by sex and BW and then randomly assigned to either the exercised (EX) or non-exercised (NEX) treatment. Diets were formulated to meet or exceed NRC requirements for yearlings in moderate growth. Diets were fed at 2.5% of BW which consisted of a pelleted concentrate, prairie grass hay, and alfalfa cubes in a 50:30:20 ratio, respectively. Radiographs of the third metacarpal were taken on d 0, d 45 and d 90 for determination of radiographic bone aluminum equivalence (RBAE). Blood was taken every 14d throughout the trial to measure serum ICTP and PICP concentrations. Although no differences between treatments were found in RBAE within days measured, a significant decrease in RBAE was observed between d 0 and 90 for the NEX horses. This decrease in bone optical density throughout the duration of the trial would indicate that stalling and lack of exercise may result in a greater loss of bone mineral content as compared to the horses forced to exercise. Consequently, the EX horses may be better prepared for future mechanical stresses placed upon them.

Impacts
Data from this trial may better aid in defining the exercise requirements for young, growing horses in order to optimize bone growth and integrity.

Publications

  • J.C. Henry, S.R. Cooper, D.W. Freeman and J.R. Kropp. 2003. Effects of Exercise on Bone Metabolism in Yearling Horses. Prof. Anim. Sci. 19: 435-438.


Progress 10/01/01 to 09/30/02

Outputs
Sixteen yearling Quarter Horses were used in a split plot designed experiment to determine the effect of exercise on bone metabolism. Horses were blocked by age and weight, and then randomly assigned to either the exercised (EX) or non-exercised (NEX) treatment. Diets were formulated to meet or exceed NRC requirements for yearlings in moderate growth. Diets consisted of a pelleted concentrate, prairie grass hay, and alfalfa cubes fed in a 50:30:20 ratio at 2.5% of body weight. The 90-d trial consisted of three 72-h collection periods on d 0 (period I), d 45 (period II), and d 90 (period III) during which total fecal collections were performed. Radiographs of the third metacarpal were also taken at this time. Body weight and skeletal parameters were measured every 7-d and blood was taken every 14-d for determination of serum ICTP and PICP concentrations. Although no differences between treatments were found in RBAE within periods, changes in RBAE across periods demonstrated a tendency for decreased bone density in both the EX and NEX horses between periods II and III. Furthermore, the significant decrease in bone density observed in the NEX horses throughout the duration of the trial would indicate that stalling and lack of exercise may result in a greater loss of BMC as compared to the forced exercise horses. Consequently, EX horses may be better prepared for the future mechanical stress placed upon them.

Impacts
Data from this trial may aid in better defining the exercise requirements for young, growing horses in order to optimize bone growth and integrity.

Publications

  • J.C. Henry, S.R. Cooper, D.W. Freeman and J.R. Kropp. 2002. Effects of Exercise on Bone Density, Growth Parameters and Biochemical Markers of Bone Metabolism in Yearling Quarter Horses. Okla. Agr. Exp. Stat. Res. Rep. http://www.ansi.okstate.edu/research/rr.htm


Progress 10/01/00 to 09/30/01

Outputs
Exp.1 Four mature sedentary geldings were used in a 4x4 Latin square design experiment to study the effects of Phytase on phosphorus availability and mineral balance. Results from this trial found that phosphorus digestibility and balance were not significantly enhanced through the addition of phytase to the diet. Exp.2 Fifteen yearling Quarter Horses were used in a split-plot design experiment to evaluate the response of calcium metabolism, bone density and parameters of growth to varying calcium concentrations in the diet. Horses were on trial from 12 to 18 months of age and were fed diets containing 115%, 100% and 85% of NRC requirement for calcium. Results from this trial demonstrate an increased calcium retention with increased intake. However, no difference was detected between dietary treatments in bone density or parameters of growth. These data therefore support current NRC recommendations for dietary calcium in yearling horses.

Impacts
Data from these trials may aid in the revision of the current NRC for Horses (1989) by better defining calcium and phosphorus requirements for young, growing horses.

Publications

  • A.D. Moffett, S.R. Cooper, D.W. Freeman and H.T. Purvis. 2001. Response of yearling Quarter Horses to varying concentrations of dietary calcium. Proc. 17th Equine Nutr. Physiol. Symp. Lexington,KY.62-68.
  • D.P. Patterson, S.R. Cooper, D.W. Freeman and R.G. Teeter. 2002. Effects of varying levels of phytase supplementation on nutrient digestibility in horses fed a common textured ration. J. Equine Vet. Sci. (Submitted).


Progress 10/01/99 to 09/30/00

Outputs
This project was new October 2000 and there is no progress to report this period.

Impacts
(N/A)

Publications

  • No publications reported this period