Veterinary Genetics Laboratory - UNIV OF CALIFORNIA (VET-MED)

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
304	3399	1080	50%
304	3499	1080	40%
304	3810	1080	10%

Progress 01/01/11 to 12/31/11

Outputs
OUTPUTS: The Veterinary Genetics Laboratory (VGL) is the largest veterinary genetics service laboratory in the world, and conducts over 150,000 individual genetic tests each year. Over 80% of these tests involve parentage and various horse registries are the largest clients of the laboratory. However, the VGL also provides service to a wide number of animal registries representing species ranging from mice, cats, dogs, llamas/alpacas, cattle, deer, elk, to bison. The laboratory is divided into several units, each with its own unit leader; these include canine, feline, wildlife, livestock, animal forensics, informatics, and service units. Funds for research come from service income, intramural grants, and extramural grants. The Veterinary Genetics Laboratory contributed $466,325 in direct faculty salaries and benefits, and $20,373 in graduate student support from service fees. In 2011, the Veterinary Genetics Laboratory's principal investigators were awarded $529,798 in extramural grants and had 31 publications.Information regarding service offerings and current research projects can be found on their website at http://www.vgl.ucdavis.edu/ PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Purebreed animal registries, individual animal owners, veterinarians. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The mission of the Veterinary Genetics Laboratory is to contribute to the academic plan of the campus and the School of Veterinary Medicine in providing genetic services to the animal owning public and industries, research on comparative animal/human genetics as a benefit to both man and animals, and the training of Master's and Ph.D. students in modern comparative genomics. The VGL is a leader in the development and application of genetics to veterinary-based forensics. Faculty of the Veterinary Genetics Laboratory include Cecilia Torres-Penedo, Holly Ernest, Ben Sacks, Niels Pedersen, Danika Bannasch, Leslie Lyons.

Publications

Delgado JV, Martinez AM, Acosta A, Alvarez LA, Armstrong E, Camacho E, Canon J, Cortes O, Dunner S, Landi V, Marques JR, Martin-Burriel I, Martinez OR, Martinez RD, Melucci L, Munoz JE, Penedo MCT, Postiglioni A, Quiroz J, Rodellar C, Sponenberg P, Uffo O, Ullowa-Arvizu R, Vega-Pla JL, Villalobos A, Zambrano D, Zaragoza P, Gama LT, Ginja C. Genetic characterization of Latin-American Creole cattle using microsatellite markers. Animal Genetics 2011: doi: 10.1111/j.1365-2052.2011.02207.x. Epub 2011 May 27.
Brault LS, Cooper CA, Famula TR, Murray JD, Penedo MCT. Mapping of equine cerebellar abiotrophy to ECA2 and identification of a potential causative mutation affecting expression of MUTYH. Genomics 2011, 97:121-129.
Edwards CJ, Ginja C, Kantanen J, Perez-Pardal L, Tresset A, Stock F; European Cattle Genetic Diversity Consortium, Gama LT, Penedo MC, Bradley DG, Lenstra JA, Nijman IJ. Dual origins of dairy cattle farming--evidence from a comprehensive survey of European Y-chromosomal variation. PLoS One. 2011 Jan 6;6(1):e15922.
Boyce WM, Weisenberger ME, Penedo MCT, Johnson CK. Wildlife translocation: the conservation implications of pathogen exposure and genetic heterozygosity. BMC Ecology, 2011, 11:5 (doi:10.1186/1472-6785-11-5).
Brault LS, Penedo MCT. The frequency of the equine cerebellar abiotrophy mutation in non-Arabian horse breeds. Equine Vet J 2011, 43(6):727-731.
Johnson ZP, Eady RD, Ahmad SF, Agravat S, Morris T, Else J, Lank SM, Wiseman RW, O'Connor DH, Penedo MC, Larsen CP, Kean LS. Immunogenetic Management Software: a new tool for visualization and analysis of complex immunogenetic datasets. Immunogenetics. 2011, Nov 15. Epub ahead of print.
Safra N, Pedersen NC, Wolf Z, Johnson EG, Liu HW, Hughes AM, Young A, Bannasch DL. Expanded dog leukocyte antigen (DLA) single nucleotide polymorphism (SNP) genotyping reveals spurious class II associations. Vet J, 189(2): 220-6. 2011
Finno CJ, Higgins RJ, Aleman M, Ofri R, Hollingsworth SR, Bannasch DL, Reilly CM, Madigan JE. Equine Degenerative Myeloencephalopathy in Lusitano horses. J Vet Intern Med, 25(6): 1439-1446. 2011
Brown SK, Pedersen NC, Jafarishorijeh S, Bannasch DL, Ahrens KD, Wu J-T, Okon M, Sacks BN. Phylogenetic Distinctiveness of Middle Eastern and Southeast Asian Village Dog Y Chromosomes Illuminates Dog Origins. PLoS ONE, 6(12): e28496. 2011
McCue ME, Bannasch DL, Petersen JL, Gurr J, Bailey E, Binns MW, Distl O, Guerin G, Hasegawa T, Hill EW, Leeb T, Lindgren G, Penedo MCT, Roed K, Ryder O, Swinburne J, Tozaki T, Valberg SJ, Vaudin M, Lindblad-Toh K, Wade CM, Mickelson JR. A High Density SNP Array for the Domestic Horse and Extant Perissodactyla: Utility for Association Mapping, Genetic Diversity and Phylogeny Studies. PLos Genetics. 2011
Bollmer JL, Hull JM, Ernest HB, Sarasola JH, Parker PG. Reduced MHC and neutral variation in the Galapagos hawk, an island endemic. BMC Evolutionary Biology,11(1):143. 2011
Jepsen EP, Keane J, Ernest HB. Winter Distribution and Conservation Status of the Sierra Nevada Great Gray Owl. 2011.Journal of Wildlife Management,75(8):1678-1687
Pelligrini AR, Wright S, Reisen WK, Treiterer B, Ernest HB. Annual Survival of House Finches in Relation to West Nile Virus. The Condor, 113(1):233-238. 2011
Sacks BN, Moore M, Statham MJ, Wittmer HU. A restricted hybrid zone between native and introduced red fox (Vulpes vulpes) populations suggests reproductive barriers and competitive exclusion. Molecular Ecology, 20:326-341. 2011
Statham MJ, Trut L, Sacks BN, Kharlamova A, Oskina I, Gulievich R, Johnson J, Temnykh S, Acland G, Kukekova A. On the origin of a domesticated species: Identifying the parent population of Russian silver foxes (Vulpes vulpes). Biological Journal of the Linnean Society, 103(1):168-175. 2011
Brown SK, Pedersen NC, Jafarishorijeh S, Bannasch DL, Ahrens KD, Wu J-T, Okon M, Sacks BN. Phylogenetic Distinctiveness of Middle Eastern and Southeast Asian Village Dog Y Chromosomes Illuminates Dog Origins. PLoS ONE, 6(12):1-9. 2011
Statham MJ, Sacks BN, Aubry KA, Perrine JD, Wisely SM. The origin of recently established red fox populations in the United States: Translocations or natural range expansions. Journal of Mammalogy. 2011
Pedersen NC, Liu H, Millon L, Greer K. Dog leukocyte antigen class II- associated genetic risk testing for immune disorders of dogs: simplified approaches using pug dog necrotizing encephalitis as a model. J Vet Diagn Invest 23:000-000, 2011. Epub
Safra N, Pedersen NC, Wolf Z, Johnson EG, Liu HW, Hughes AM, Young A, Bannasch DL. Expanded dog leukocyte antigen (DLA) single nucleotide polymorphism (SNP)genotyping reveals spurious class II associations. Vet. J. 2011, 189,220-226.
Brown SK, Pedersen NC, Jafarishorijeh S, Bannasch DL, Ahrens KD, Wu J-T, Okon M, Sacks BN. Phylogenetic Distinctiveness of Middle Eastern and Southeast Asian Village Dog Y Chromosomes Illuminates Dog Origins. PLoS ONE. 2011
Canon J, D. Garcia D, Delgado JV, Dunner S, Telo da Gama L, Landi V, Martin-Burriel I, Martinez A, Penedo, C, Rodellar C, Zaragoza P and Ginja C. Relative breed contributions to neutral genetic diversity of a comprehensive representation of Iberian native cattle. Animal, 2011: doi:10.1017/S1751731111000267. 2011
Boyce WM, Weisenberger ME, Penedo MC, Johnson CK. Wildlife translocation: the conservation implications of pathogen exposure and genetic heterozygosity. BMC Ecol. 2011, 11:5. 2011
Vaysse A, Ratnakumar A, Derrien T, Axelsson E, Pielberg GR, Sigurdsson S, Fall T, Seppala E, Hansen MST, Lawley CT, Karlsson EK, Bannasch DL, Vila C, Lohi H, Galibert F, Fredholm M, Hedhammar A, Andre C, Lindblad-Toh K, Hitte C, Webster MT. Identification of genomic regions associated with phenotypic variation between dog breeds using selection mapping. Plos Genetics. 2011
Leonard BC, Marks SL, Outerbridge CA, Affolter VK, Kananurak A, Young A, Moore PF, Bannasch DL, Bevins CL. Activity, Expression and Genetic Variation of Canine beta-Defensin 103: A Multifunctional Antimicrobial Peptide in the Skin of Domestic Dogs. Journal of Innate Immunity. 2011
Grahn RA, Kurushima JD, Billings NC, Grahn JC, Halverson JL, Hammer E, Ho CK, Kun TJ, Levy JK, Lipinski MJ, Mwenda JM, Ozpinar H, Schuster RK, Shoorijeh SJ, Tarditi CR, Waly NE, Wictum EJ, Lyons LA. Feline non-repetitive mitochondrial DNA control region database for forensic evidence. Forensic Sci Int Genet, Jan; 5(1):33-42. 2011
Tarditi CR, Grahn RA, Evans JJ, Kurushima JD, Lyons LA. Mitochondrial DNA sequencing of cat hair: an informative forensic tool. J Forensic Sci, Jan; 56(Suppl s1): S36-46. 2011
Schlafer DH, Valentine B, Fahnestock G, Froenicke L, Grahn RA, Lyons LA, Meyers-Wallen VN.A case of SRY-positive 38,XY true hermaphroditism (XY sex reversal) in a cat. Vet Pathol, Jul; 48(4):817-22. 2011
Martin-Burriel I, Rodellar C, Canon J, Cortes O, Dunner S, Landi V, Martinez-Martinez A, Gama LT, Ginja C, Penedo MC, Sanz A, Zaragoza P, Delgado JV. Genetic diversity, structure, and breed relationships in Iberian cattle. J Anim Sci. 2011,89(4):893-906.
Haase B, Rieder S, Tozaki T, Hasegawa T, Penedo MC, Jude R, Leeb T. Five novel KIT mutations in horses with coat color phenotypes. Anim Genet 2011, 42(3):337-339.
Brault LS, Famula TR, Penedo MCT. Inheritance of cerebellar abiotrophy in Arabians. Am J Vet Res 2011,72(7):940-944.
Perez-Pardal L, Ginja C, Royo LJ, Alvarez I, Fernandez I, del Valle A, Traore A, Ponce de Leon FA, Beja-Pereira A, Penedo MC, Goyache F. Genetic structure of the bovine Y-specific microsatellite UMN0103 reflects the genetic history of the species. Animal Genetics. 2011, 42(5): 566-567.

Progress 01/01/10 to 12/31/10

Outputs
OUTPUTS: The Veterinary Genetics Laboratory (VGL) is the largest veterinary genetics service laboratory in the world, and conducts over 150,000 individual genetic tests each year. Over 80% of these tests involve parentage and various horse registries are the largest clients of the laboratory. However, the VGL also provides service to a wide number of animal registries representing species ranging from mice, cats, dogs, primates, llamas/alpacas, cattle, deer, elk, to bison. The laboratory is divided into several units each with its own unit leader; these include canine, feline, wildlife, livestock, animal forensics, and informatics and service units. Funds for research come from service income, intramural grants, and extramural grants. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Purebred animal registries; individual animal owners, veterinarians. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Purebreed animal registries, individual animal owners, veterinarians. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The mission of the Veterinary Genetics Laboratory (VGL) is to contribute to the academic plan of the campus and the School of Veterinary Medicine in providing genetic services to the animal owning public and industries, research on comparative animal/human genetics as a benefit to both man and animals and the training of Master's and Ph.D. students in modern comparative genomics. The VGL is a leader in the development and application of genetics to veterinary-based forensics. Faculty of the Veterinary Genetics Laboratory include Cecilia Torres-Penedo, Holly Ernest, Ben Sacks, Niels Pedersen, Danika Bannasch, Leslie Lyons.

Publications

Brault LS, Famula TR, Penedo MCT. Inheritance of equine cerebellar abiotrophy in Arabian horses. 2010. American Journal of Veterinary Research
Brault LS, Penedo MCT. The frequency of the equine cerebellar abiotrophy mutation in non-Arabian horse breeds. 2010. Equine Veterinary Journal
Brault LS, Cooper CA, Famula TR, Murray JD, Penedo MCT. Mapping of equine cerebellar abiotrophy to ECA2 and identification of a potential causative mutation affecting expression of MUTYH. 2010. Genomics
Haase B, Rieder S, Tozaki T, Hasegawa T, Penedo MCT, Jude R, Leeb T. Five novel KIT mutations in horses with white coat colour phenotypes. 2010. Animal Genetics
Andrews GA, Penedo MCT: Erythrocyte antigens and Blood Groups, Douglas J. Weiss, K. Jane Wardrop, (ed), 2010. Schalm's Veterinary Hematology, 6th edition, Wiley-Blackwell, Singapore. Chapter 92: 711-724
Moore, M, SK Brown, BN Sacks. Thirty-one short red fox (Vulpes vulpes) microsatellite markers. 2010. Molecular Ecology Resources, 10: 404-408
Sacks, BN, MJ Statham, JD Perrine, SM Wisely, KA Aubry. North American montane red foxes: expansion, fragmentation, and the origin of the Sacramento Valley red fox. 2010. Conservation Genetics, 11: 1523-1539
Sacks, B. N., Wittmer H. U. and Statham, M. J.: The Native Sacramento Valley red fox: Report to the California Department of Fish and Game, May 30, 2010
Karmi N, Safra N, Young A, Bannasch DL. Validation of a urine test and characterization of the putative genetic mutation for hyperuricosuria in Bulldogs and Black Russian Terriers. 2010. Am J Vet Res, 71(8): 909-14
Hughes AM, Jokinen P, Bannasch DL, Lohi H, Oberbauer AM. Association of a dog leukocyte antigen class II haplotype with hypoadrenocorticism in Nova Scotia Duck Tolling Retrievers. 2010. Tissue Antigens, 75(6): 684-90
Vonholdt BM, Pollinger JP, Lohmueller KE, Han E, Parker HG, Quignon P, Degenhardt JD, Boyko AR, Earl DA, Auton A, Reynolds A, Bryc K, Brisbin A, Knowles JC, Mosher DS, Spady TC, Elkahloun A, Geffen E, Pilot M, Jedrzejewski W, Greco C, Randi E, Bannasch D, Wilton A, Shearman J, Musiani M, Cargill M, Jones PG, Qian Z, Huang W, Ding ZL, Zhang YP, Bustamante CD, Ostrander EA, Novembre J, Wayne RK. Genome-wide SNP and haplotype analyses reveal a rich history underlying dog domestication. 2010. Nature, 464(7290): 898-902
Bannasch D, Young A, Myers J, Truve K, Dickinson P, Gregg J, Davis R, Bongcam-Rudloff E, Webster MT, Lindblad-Toh K, Pedersen N. Localization of canine brachycephaly using an across breed mapping approach. 2010. PLoS One, 5(3): e9632
Wilbe M, Jokinen P, Truve K, Seppala EH, Karlsson EK, Biagi T, Hughes A, Bannasch D, Andersson G, Hansson-Hamlin H, Lohi H, Lindblad-Toh K. Genome-wide association mapping identifies multiple loci for a canine SLE-related disease complex. 2010. Nat Genet, 42(3): 250-4
Kinnally EL, Karere GM, Lyons LA, Mendoza SP, Mason WA, Capitanio JP. Serotonin pathway gene-gene and gene-environment interactions influence behavioral stress response in infant rhesus macaques. 2010. Dev Psychopathol, 22(1): 35-44
Karere GM, Lyons LA, Froenicke L. Enhancing radiation hybrid mapping through whole genome amplification. 2010. Hereditas, 147(2): 103-12
Grahn RA, Kurushima JD, Billings NC, Grahn JC, Halverson JL, Hammer E, Ho CK, Kun TJ, Levy JK, Lipinski MJ, Mwenda JM, Ozpinar H, Schuster RK, Shoorijeh SJ, Tarditi CR, Waly NE, Wictum EJ, Lyons LA. Feline non-repetitive mitochondrial DNA control region database for forensic evidence. 2010. Forensic Sci Int Genet, 5(1): 33-42
Schlafer DHValentine BFahnestock GFroenicke LGrahn RALyons LAMeyers-Wallen VN. A Case of SRY-Positive 38,XY True Hermaphroditism (XY Sex Reversal) in a Cat. 2010. Vet Pathol, 509-15
Gandolfi B, Outerbridge CA, Beresford LG, Myers JA, Pimentel M, Alhaddad H, Grahn JC, Grahn RA, Lyons LA. The naked truth: Sphynx and Devon Rex cat breed mutations in KRT71. 2010. Mamm Genome, 21(9-10): 509-15
Tarditi CR, Grahn RA, Evans JJ, Kurushima JD, Lyons LA. Mitochondrial DNA sequencing of cat hair: an informative forensic tool. 2010. J Forensic Sci, 56 Suppl 1: S36-46
Lyons LA. Feline genetics: clinical applications and genetic testing. 2010. Top Companion Anim Med, 25(4): 203-12
Hull JM, DP Mindell, Talbot SL, Kay EH, Hoekstra HE, and Ernest HB. Population structure and plumage polymorphism: The intraspecific evolutionary relationships of a polymorphic raptor, Buteo jamaicensis harlani. 2010. BMC Evolutionary Biology 10:224
Ginja C, Penedo MCT, Sobral MF, Matos J, Borges C, Neves D, Rangel-Figueiredo T, Cravador A. Molecular genetic analysis of a cattle population to reconstitute the extinct Algarvia breed. 2010. Genetics, Selection and Evolution, 42: 18pp
Hull JM, Keane JJ, Tell LA, Ernest HB. West Nile virus antibody surveillance in three. Sierra Nevada raptors of conservation concern. 2010. The Condor 112(1):168-172
Hull JM, Keane JJ, Savage WK, Godwin SA, Shafer J, Jepsen EP, Gerhardt R,Stermer C, and Ernest HB. Range-wide genetic differentiation among North American great gray owls(Strix nebulosa) reveals a distinct lineage restricted to the Sierra Nevada Mountains. 2010. Molecular Phylogenetics and Evolution. 56:212-221
Ernest HB, Hoar BR, Well JA, O'Rourke K. Molecular genealogy tools for white-tailed deer with Chronic Wasting Disease. 2010. Canadian Journal of Veterinary Research. 74(2)152
Ernest HB, Woods LW, Hoar BR. Pathology associated with West Nile Virus infections in the Yellow-billed Magpie (Pica nuttalli): a California endemic bird. 2010. Journal of Wildlife Diseases. 46:401-408
Ginja C, Penedo MCT, Melucci L, Quiroz J, Martinez Lopez OR, Revidatti MA, Martinez-Martinez A, Delgado JV, Gama LT. Origins and genetic diversity of New World Creole cattle: inferences from mitochondrial and Y chromosome polymorphisms. 2010. Animal Genetics, 41(2): 128-141
Greer KA, Wong AK, Liu H, Famula TR, Pedersen NC, Ruhe A, Wallace M, Neff MW. Necrotizing meningoencephalitis of Pug Dogs associates with dog leukocyte antigen class II and resembles acute variant forms of multiple sclerosis. 2010. Tissue Antigens, 76(2): 110-8
Bannasch D, Young A, Myers J, Truve K, Dickinson P, Gregg J, Davis R, Bongcam-Rudloff E, Webster MT, Lindblad-Toh K, Pedersen N. Localization of canine brachycephaly using an across breed mapping approach. 2010. PLoS One, 5(3): e9632
Pedersen NC, Liu H, Millon L, Geer KA. DLA class II associated genetic risk testing for immune disorders of dogs - Simplified approaches using Pug Dog necrotizing meningoencephalitis as a model. 2010. Journal Veterinary Laboratory Diagnostic Investigation
Ginja C, Telo Da Gama L, Penedo MCT. Analysis of STR markers reveals high genetic structure in Portuguese native cattle. 2010. Journal of Heredity, 101(2): 201-210
Kanthaswamy S, Kou A, Satkoski J, Penedo MCT, Ward T, Ng J, Gill L, Lerche NW, Erickson BJ, Smith DG. Genetic characterization of specific pathogen-free rhesus macaque (Macaca mulatta) populations at the California National Primate Research Center (CNPRC). 2010. American Journal of Primatology, 72(7): 587-599
Miller WP, Srinivasan S, Panoskaltsis-Mortari A, Singh K, Sen S, Hamby K, Deane T, Stempora L, Beus J, Turner A, Wheeler C, Anderson DC, Sharma P, Garcia A, Strobert E, Elder E, Crocker I, Crenshaw T, Penedo MCT, Ward T, Song M, Horan J, Larsen CP, Blazar BR, Kean LS. GvHD after haploidentical transplant: a novel, MHC-defined rhesus macaque model identifies CD28-negative CD8+ T cells as a reservoir of breakthrough T cell proliferation during costimulation blockade and sirolimus-based immunosuppression. 2010. Blood (epub DOI 10.1182/blood-2010-06-289272)
Larsen CP, Page A, Linzie KH, Russell M, Deane T, Stempora L, Strobert E, Penedo MCT, Ward T, Wiseman R, O'Connor D, Miller W, Sen S, Singh K, Kean LS. An MHC-Defined Primate Model Reveals Significant Rejection of Bone Marrow After Mixed Chimerism Induction Despite Full MHC Matching. 2010. American Journal of Transplantation, 10: 1-14
McCue ME, Anderson SM, Valberg SJ, Piercy RJ, Barakzai SZ, Binns MM, Distl O, Penedo MCT, Wagner ML, Mickelson JR. Estimated prevalence of the Type 1 Polysaccharide Storage Myopathy mutation in selected North American and European breeds. 2010. Animal Genetics, 41(Suppl 2): 145-149

Progress 01/01/09 to 12/31/09

Outputs
OUTPUTS: The Veterinary Genetics Laboratory (VGL) is the largest veterinary genetics service laboratory in the world, and conducts over 180,000 individual genetic tests each year. Over 80% of these tests involve parentage and various horse registries are the larges clients of the laboratory. However, the VGL also provides service to a wide number of animal registries representing species ranging from mice, cats, dogs, primates, llamas/alpacas, cattle, deer, elk, to bison. The laboratory is divided into several units each with its own unit leader; these include canine, feline, wildlife, livestock, animal forensics, and informatics and service units. Funds for research come from service income, intramural grants, and extramural grants. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Purebreed animal registries; individual animal owners, veterinarians. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The mission of the Veterinary Genetics Laboratory (VGL) is to contribute to the academic plan of the campus and the School of Veterinary Medicine in providing genetic services to the animal owning public and industries, research on comparative animal/human genetics as a benefit to both man and animals and the training of Master's and Ph.D. students in modern comparative genomics. The VGL is a leader in the development and application of genetics to veterinary-based forensics. Faculty of the Veterinary Genetics Laboratory include Cecilia Torres-Penedo, Holly Ernest, Ben Sacks, Niels Pedersen, Danika Bannasch, Leslie Lyons.

Publications

Tryon RC, Penedo MC, McCue ME, Valberg SJ, Mickelson JR, Famula TR, et al. 2009. Evaluation of allele frequencies of inherited disease genes in subgroups of American Quarter Horses. J Am Vet Med Assoc. 2009 Jan 1, 234(1): 120-5.
Bannasch D, Henthorn PS. 2009. Changing paradigms in diagnosis of inherited defects associated with urolithiasis. Vet Clin North Am Small Anim Pract. 2009 Jan;39(1): 111-25.
Hughes AM, Bannasch DL, Kellett K, Oberbauer AM. 2009. Examination of candidate genes for hypoadrenocorticism in Nova Scotia Duck Tolling Retrievers. Vet J. 2009 Nov 18.
Hull JM, Ernest HB, Harley JA, et al. 2009. Differential migration between discrete populations of juvenile Red-tailed Hawks (Buteo jamaicensis). Journal of the American Ornithologists Union, 2009; 126(2): 389-396.
Kanthaswamy S, Capitanio JP, Dubay CJ, Ferguson B, Folks T, Ha JC, et al. 2009. Resources for genetic management and genomics research on non-human primates at the National Primate Research Centers (NPRCs). Med Primatol. 2009 Oct;38 Suppl 1:17-23.
Gomez MC, Pope CE, Kutner RH, Ricks DM, Lyons LA, Ruhe MT, Dumas C, et al. 2009. Generation of domestic transgenic cloned kittens using lentivirus vectors. Cloning Stem Cells. 2009 Mar;11(1):167-76.
Hung PH, Froenicke L, Lin CY, Lyons LA, Miller MG, Pinkerton KE, VandeVoort CA. 2009. Effects of environmental tobacco smoke in vivo on rhesus monkey semen quality, sperm function, and sperm metabolism. Reprod Toxicol. 2009 Apr;27(2):140-8.
Karere GM, Kinnally EL, Sanchez JN, Famula TR, Lyons LA, Capitanio JP. 2009. What is an "adverse" environment Interactions of rearing experiences and MAOA genotype in rhesus monkeys. Biol Psychiatry. 2009 May 1;65(9):770-7.
Tellgren-Roth A, Dittmar K, Massey SE, Kemi C, Tellgren-Roth C, Savolainen P, Lyons LA, Liberles DA. 2009. Keeping the blood flowing-plasminogen activator genes and feeding behavior in vampire bats. Naturwissenschaften. 2009 Jan;96(1):39-47.
Hung PH, Froenicke L, Lyons LA, VandeVoort CA. 2009. Nothing 'FISH'y about the rhesus macaque sex ratio. J Med Primatol. 2009 Feb;38(1):42-50.
Wade CM, Giulotto E, Sigurdsson S, Zoli M, Gnerre S, Imsland F, Lear TL, Adelson DL, et al. 2009. Genome sequence, comparative analysis, and population genetics of the domestic horse. Broad Institute Genome Sequencing Platform; Broad Institute Whole Genome Assembly Team, Science. 2009 Nov 6;326(5954):865-7.
Ginja C, Penedo MC, Melucci L, Quiroz J, et al. 2009. Origins and genetic diversity of New World Creole cattle: inferences from mitochondrial and Y chromosome polymorphisms. Anim Genet. 2009 Oct 9.
Maga EA, Daftari P, Kultz D, Penedo MC. 2009. Prevalence of alphas1-casein genotypes in American dairy goats. J Anim Sci. 2009 Nov;87(11): 3464-3469.
Tryon RC, Penedo MC, McCue ME, Valberg SJ, Mickelson JR, Famula TR, et al. 2009. Evaluation of allele frequencies of inherited disease genes in subgroups of American Quarter Horses. J Am Vet Med Assoc. 2009 Jan 1;234(1):120-5.
Ginja C, Telo da Gama L, Penedo MC. 2009. Y chromosome haplotype analysis in Portuguese cattle breeds using SNPs and STRs. J Hered. 2009 Mar-Apr;100(2):148-57.
Haase B, Brooks SA, Tozaki T, Burger D, et al. 2009. Seven novel KIT mutations in horses with white coat color phenotypes. Anim Genet. 2009 Oct;40(5):623-9.
Simerly CR, Navara CS, Castro CA, Turpin JC, Redinger CJ, Mich-Basso JD, et al. 2009. Establishment and characterization of baboon embryonic stem cell lines: An Old World Primate model for regeneration and transplantation research. Stem Cell Res. 2009 Feb 21.
Pedersen NC. 2009. A review of feline infectious peritonitis virus infection: 1963-2008. J Feline Med Surg. 2009 Apr;11(4):225-58.
Aubry KB, Statham MJ, Sacks BN, Perrine JD, Wisely SM. 2009. Phylogeography of the North American red fox: vicariance in Pleistocene forest refugia. Mol Ecol. 2009 Jun;18(12):2668-86.
Sacks, BN, DI Vage, MJ Statham. 2009. A medium-throughput SNP assay for detecting genetic variation in coding and non-coding portions of the red fox genome. Conservation Genetics Resources, 2009; 1: 459-463.

Progress 01/01/08 to 12/31/08

Outputs
OUTPUTS: The Veterinary Genetics Laboratory (VGL) is the largest veterinary genetics service laboratory in the world, and conducts over 200,000 individual genetic tests each year. Over 80% of these tests involve parentage and various horse registries are the largest clients of the laboratory. However, the VGL also provides service to a wide number of animal registries representing species ranging from mice, cats, dogs, llamas/alpacas, cattle, deer, elk, to bison. The laboratory is divided into several units, each with its own unit leader; these include canine, feline, wildlife, livestock, animal forensics, informatics, and service units. Funds for research come from service income, intramural grants, and extramural grants. Information regarding service offerings and current research projects can be found on their website at http://www.vgl.ucdavis.edu/ PARTICIPANTS: No Participants information reported. TARGET AUDIENCES: No Target Audiences information reported. PROJECT MODIFICATIONS: No Project Modifications information reported.

Impacts
The mission of the Veterinary Genetics Laboratory is to contribute to the academic plan of the campus and the School of Veterinary Medicine in providing genetic services to the animal owning public and industries, research on comparative animal/human genetics as a benefit to both man and animals, and the training of Master's and Ph.D. students in modern comparative genomics.

Publications

No publications reported this period

Progress 01/01/07 to 12/31/07

Outputs
The Veterinary Genetics Laboratory (VGL) is the largest veterinary genetics service laboratory in the world, and conducts over 200,000 individual genetic tests each year. Over 80% of these tests involve parentage and various horse registries are the largest clients of the laboratory. However, the VGL also provides service to a wide number of animal registries representing species ranging from mice, cats, dogs, llamas/alpacas, cattle, deer, elk, to bison. The laboratory is divided into several units, each with its own unit leader; these include canine, feline, wildlife, livestock, animal forensics, informatics, and service units. Funds for research come from service income, intramural grants, and extramural grants. Information regarding service offerings and current research projects can be found on their website at http://www.vgl.ucdavis.edu/ The Veterinary Genetics Laboratory contributed $350,000 in direct faculty salaries and benefits, and $286,800 in graduate student support from service fees. In 2007, the Veterinary Genetics Laboratory's principal investigators were awarded $933,425 in extramural grants and $55,000 in intramural grants and had 7 publications.

Impacts
The mission of the Veterinary Genetics Laboratory is to contribute to the academic plan of the campus and the School of Veterinary Medicine in providing genetic services to the animal owning public and industries, research on comparative animal/human genetics as a benefit to both man and animals, and the training of Master's and Ph.D. students in modern comparative genomics.

Publications

No publications reported this period

Progress 01/01/06 to 12/31/06

Outputs
The Veterinary Genetics Laboratory (VGL) is the largest veterinary genetics service laboratory in the world, and conducts over 200,000 individual genetic tests each year. Over 80% of these tests involve parentage and various horse registries are the largest clients of the laboratory. However, the VGL also provides service to a wide number of animal registries representing species ranging from mice, cats, dogs, llamas/alpacas, cattle, deer, elk, to bison. The laboratory is divided into several units, each with its own unit leader; these include canine, feline, wildlife, livestock, animal forensics, informatics, and service units. Funds for research come from service income, intramural grants, and extramural grants. Information regarding service offerings and current research projects can be found on their website at http://www.vgl.ucdavis.edu/ The Veterinary Genetics Laboratory contributed $332,000 in direct faculty salaries and benefits, and $24,238 in graduate student support from service fees. In 2006, the Veterinary Genetics Laboratory's principal investigators were awarded $618,337 in extramural grants and $110,200 in intramural grants and had 15 publications.

Impacts
The mission of the Veterinary Genetics Laboratory is to contribute to the academic plan of the campus and the School of Veterinary Medicine in providing genetic services to the animal owning public and industries, research on comparative animal/human genetics as a benefit to both man and animals, and the training of Master's and Ph.D. students in modern comparative genomics.

Publications

No publications reported this period

Progress 01/01/05 to 12/31/05

Outputs
The Veterinary Genetics Laboratory conducts research in the areas of 1) Companion animal research and development, 2) genomic research and development, 3) wildlife and ecology,4)forensics, and 5) informatics. Information regarding the current research projects can be found on their website at http://www.vgl.ucdavis.edu/ The Veterinary Genetics Laboratory contributed $350,000 in direct faculty salaries and benefits, and $286,800 in graduate student support from service fees. In 2005, the Veterinary Genetics Laboratory's principal investigators were awarded $933,425 in extramural grants and $55,000 in intramural grants and had 7 publications.

Impacts
The mission of the Veterinary Genetics Laboratory is to contribute to the academic plan of the campus and the School of Veterinary Medicine in providing research, service and teaching pertaining to the genetic component of animal health.

Publications

Foley, J. E., E, V, Queen, B. N. Sacks, P. Foley. 2005. GIS-facilitated spatial epidemiology of tick-borne diseases of coyotes (Canis latrans) in northern and coastal California. Comparative Immunology, Microbiology, & Infectious Diseases. 28:197-212.
Beldomenico, P. E., B. B. Chomel, J. E. Foley, B. N. Sacks, C. J. Baldi, R. W. Kasten, I. A. Gardner. 2005. Environmental factors associated with /Bartonella vinsonii subsp. berkhoffii seropositivity in ree-ranging coyotes from northern California. Journal of Vector Ecology. In press
Tozaki T, MCT Penedo, RP Oliveira, JP Katz, LV Millon, T Ward, DC Pettegrew, LS Brault, M Tomita, T Hasegawa and K Hirota. 2005. Isolation, characterization and chromosome assignment of 341 newly isolated TKY microsatellite markers. Animal Genetics.
MCT Penedo, LV Millon, D Bernoco, E Bailey, M Binns, G. Cholewinski, N. Ellis, J. Flynn, B. Gralak, A. Guthrie, T. Hasegawa, G. Lindgren, L.A. Lyons, K.H. Roed, J.E. Swinburne and T. Tozaki. 2005. International Equine Gene Mapping Workshop Report: A comprehensive linkage map constructed with data from new markers and by merging four mapping resources. Cytogenetic and Genome Research.
Sacks, B. N., B. R. Mitchell, C. L. Williams, and H. B. Ernest. 2005. Coyote movements and social structure along a cryptic population genetic subdivision. Molecular Ecology. 14:1241-1249.
Sacks, B. N. 2005. Reproduction and body condition in California coyotes (Canis latrans). Journal of Mammalogy. 86:1036-1041.
Padgett, K. A., S. A. Nadler, L. Munson, B. N. Sacks, and W. M. Boyce. 2005. Systematics of Mesocestoides/ (Cestoda: Mesocestoididae): Evaluation of molecular and morphological variation among isolates. Journal of Parasitology 91:1435-1443.

Progress 01/01/04 to 12/31/04

Outputs
The Veterinary Genetics Laboratory conducts research in the areas of 1) Companion animal research and development, 2) genomic research and development, 3) primate population genetics, 4) wildlife and ecology 5) forensics, and 6) informatics. Information regarding the current research projects can be found on their website at http://www.vgl.ucdavis.edu/

Impacts
The mission of the Veterinary Genetics Laboratory is to contribute to the academic plan of the campus and the School of Veterinary Medicine in providing research, service and teaching pertaining to the genetic component of animal health. The Veterinary Genetics Laboratory contributed $425,000 in direct faculty salaries and benefits, and $150,000 in graduate student support from service fees. In 2004, the Veterinary Genetics Laboratory's principal investigators were awarded $514,000 in extramural grants and $63,000 in intramural grants and had 10 publications.

Publications

Sacks BN, SK Brown and HB Ernest. 2004. Population structure of California coyotes corresponds to habitat-specific breaks and illuminates species history. Molecular Ecology 13:1265-1275.
Sacks BN, JM Hull and HB Ernest. 2004. Why Ys are wise: Y chromosomes as mammalian population genetics markers. Submitted September 2004. Molecular Ecology.
Sacks BN, BR Mitchell, CL Williams and HB Ernest. 2004. Movements and social structure along a cryptic population genetic subdivision in the coyote. Submitted August 2004. Molecular Ecology.
Kanthaswamy S and DG Smith. 2004. Effects of geographic origin on captive Macaca mulatta mitochondrial DNA variation. Journal of Comparative Medicine, 54(2):166-174.
Neff MW, KR Robertson, AK Wong, N Safra, KW Broman, M Slatkin, KL Measley and NC Pedersen. 2004. On the Cover: Breed distribution and history of canine mdr1-1, a pharmacogenetic mutation that marks the emergence of breeds from the collie lineage. Proceedings of the National Academy of Sciences 101(32)11725-11730.
Rui R, H Shim, AL Moyer, DL Anderson, MCT Penedo, JD Rowe, RH BonDurant and GB Anderson. 2004. Attempts to enhance production of porcine chimeras from embryonic germ cells and preimplantation embryos. Theriogenology 61:1225-1235.
Honaramooz A, M-W Li, MCT Penedo, S Meyers and I Dobrinski. 2004. Accelerated maturation of primate testis by xenografting into mice. Biology of Reproduction 70:1500-1503.
Mateus JC, MCT Penedo, VC Alves, M Ramos and T Rangel-Figueiredo. 2004. Genetic diversity and differentiation in Portuguese cattle breeds using microsatellites. Animal Genetics 35:106-113.
Andrade MCR, MCT Penedo, T Ward, VF Silva, LR Bertolini, JA Roberts, JPG Leite and PH Cabello. 2004. Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta). Primates 45:183-186.
Mateus JC, H Eding, MCT Penedo and MT Rangel-Figueiredo. 2004. Contributions of Portuguese cattle breeds to genetic diversity using marker-estimated kinships. Animal Genetics 35:305-313.

Progress 01/01/03 to 12/31/03

Outputs
The Veterinary Genetics Laboratory (VGL) was established in the 1950's for the purpose of parentage verification for cattle registries and was originally known as the Serology Laboratory. At that time all identity and parentage testing was carried out with blood typing analysis of serum proteins and red blood cell surface proteins. Blood typing was developed for horses in the 1960s and llamas and alpacas in the 1980s. Today, under the direction of Dr. Niels Pedersen, the laboratory provides animal parentage verification, identification, forensics services, genetic diagnostics and genetic disease research as a self-supporting unit of the School of Veterinary Medicine, University of California, Davis. VGL was a pioneer in the development of DNA based animal parentage verification and in the early 1990s began development of parentage tests using microsatellite DNA markers also referred to, as STRs. VGL was the first animal parentage laboratory to offer DNA testing to the horse, cattle and camelid industries in the mid-1990s and has since introduced DNA based tests for elk, deer, dogs, cats, sheep, goats and primates. Utilizing this technology the VGL has, since the mid-1990s, generated nearly one million DNA profiles. The International Society of Animal Genetics (ISAG) recognizes the major parentage tests offered by VGL as international standards. The laboratory has been an active member of the ISAG since 1974 and takes part in the biannual horse, cattle, sheep/goat and dog parentage comparison tests carried out under the auspices of ISAG. The biannual ISAG meeting provides a venue for comparison of data and establishment of test standards followed by all animal parentage laboratories. The VGL has trained visiting scientists and technicians from laboratories in the US and abroad since the 1970s in both blood typing and DNA typing technologies. The lab is now considered the leading animal DNA typing lab in the world in the area of parentage verification. The Service Unit of VGL has contracts with a variety of breed registries/associations. VGL began development of a small-scale animal forensics service program in the mid-1990s that handled minor civil cases. This program has continued to expand and is now involved in high profile criminal cases both nationally and internationally. For several decades VGL has been a leader in equine genetic research and more recently in the international equine gene map project. The laboratory has developed and currently offers diagnostic tests for a number of equine genetic diseases and coat colors and has an active research and development program in this area.

Impacts
The mission of the Veterinary Genetics Laboratory is to contribute to the academic plan of the campus and the School of Veterinary Medicine in providing research, service and teaching pertaining to the genetic component of animal health.

Publications

No publications reported this period

Progress 01/01/02 to 12/31/02

Outputs
VGL has made significant advances in phasing in Short Tandem Repeat (STRs) markers for equine parentage verification. The development of this technology over the past 3 years has made VGL an international leader in automated DNA analysis and computerized data management. VGL is currently involved in collaborative efforts with the international equine typing community to incorporate the best of the available STRs into a standardized equine DNA typing program. Economically important traits such as desirable milk protein genes in dairy cattle are also identified by DNA tests. Clinical problems such as freemartin calves, chromosomal defects, and hyperkalemic periodic paralysis in Quarter Horses can be diagnosed. DNA tests can be used to eliminate from breeding programs the parents of offspring with genetic deformities such as rat tail in cattle. Both favorable and unfavorable traits will be detectable as DNA technology evolves other traits . VGL is also involved in collaborative efforts aimed at the isolation and characterization of additional STR markers to be utilized in equine genomic mapping and linkage studies. The information from these studies will ultimately enable VGL to provide their clients with genetic disease diagnosis as well as genetic trait screening and selection. The Veterinary Genetics Laboratory is involved in various wildlife projects that require genetic marker testing. The Prezwalski's Horse is considered to be extinct in the wild, and the 800 animals living in zoos and reserve parks are descended from only 12 wild horses. An international cooperative breeding program aims to preserve genetic variation among the Prezwalski's Horses and minimizes in-breeding by monitoring pedigrees. The Veterinary Genetics Laboratory is engaged in testing for wildlife population studies. In collaboration with Professor Walter Boyce of the Department of Pathology, Microbiology & Immunology, the laboratory is providing DNA typing tests to characterize bighorn sheep populations for a large study that will contribute to population management. In another collaborative endeavor, with biologists of the Sacramento Bufferlands Project, the laboratory is performing DNA fingerprinting analyses of beavers in the Sacramento area to determine genetic similarities among animals. VGL is the only laboratory in the world doing blood typing for llamas and alpacas for parentage verification. With funding from the Llama Association of North America, VGL in association with Professor Emeritus Murray Fowler, produced a panel of distinctive genetic markers now routinely used for parentage tests on these species. The Veterinary Genetics Laboratory is a resource for California, the Nation, and the world-at-large, and will continue to provide an important service as even more DNA-based diagnostic techniques for domestic and wildlife species and animal diseases are developed.

Impacts
The Veterinary Genetics Laboratory is internationally recognized for its expertise in parentage verification and genetic diagnostics for animals. VGL has provided services to breed registries, practitioners, individual owners and breeders since 1955.

Publications

No publications reported this period

Progress 01/01/01 to 12/31/01

Outputs
The Veterinary Genetics Laboratory began in the 1950s as a cattle blood typing laboratory providing parentage verification for breed registration as a service to the cattle industry. In the 1960s blood typing was developed for equine parentage verification as well. In the past 30 years VGL has become the largest equine blood typing facility in the world testing 50,000 to 60,000 samples per year for over 30 breed registries. The Veterinary Genetics Laboratory comprises over 40 technical, clerical and administrative staff. It is an entirely self-supporting unit of the school providing services to breed registries, practitioners and individual owners and breeders. Research focuses on equine, canine and companion animal models. Equine research centers on horse genetics, gene mapping and horse typing. The VGL is actively working on projects that contribute to the international effort to understand the canine genome. Feline research is focused on the discovery of mutations that cause inherited diseases and phenotypic traits and in the population dynamics of breed development and domestic cat evolution. Dr. Lyons has a partial appointment with the California Regional Primate Research Center (CRPRC) and is augmenting and developing new genetic resources and research for the rhesus macaque. The Comparative Genetics program is focused on the genetic mapping of specific genes in either the cat, the dog or the horse or are specific phenotypic traits or of particular biological interest to the health of humans. This research is a collaborative effort with comparative genetic researchers throughout the world.

Impacts
The Veterinary Genetics Laboratory is a resource for California, the nation, and the world-at-large, and will continue to provide an important service as even more DNA-based diagnostic techniques for domestic and wildlife species and animal diseases are developed.

Publications

No publications reported this period

Progress 01/01/00 to 12/31/00

Outputs
With the beginning of VGLs switchover to DNA typing two years ago and the additional genetic based services, the name of the facility was changed from the Serology Laboratory to the Veterinary Genetics Laboratory. Blood typing of domestic animals, which assays for erythrocyte and serum protein variants, has provided an excellent means of validating pedigrees, solving parentage in multiple sire breedings and providing identification of individual animals. While blood typing is still used in routine assays it is rapidly being replaced by DNA typing which is faster, offers higher efficacy and provides additional capabilities for genetic research. DNA tests detect changes in genomic sequences. The early generation Restriction Fragment Length Polymorphism (RFLP) and Variable Number Tandem Repeats (VNTR) DNA tests utilized by VGL for a number of species such as llamas and alpacas, produce a DNA fingerprint unique to each individual animal. VGL has made significant advances in phasing in Short Tandem Repeat (STRs) markers for equine parentage verification. VGL is currently involved in collaborative efforts with the international equine typing community to incorporate the best of the available STRs into a standardized equine DNA typing program. DNA-based genetic tests are no longer restricted to blood samples. For live animals, collecting hair roots or semen offers a non-invasive sampling procedure. Economically important traits such as desirable milk protein genes in dairy cattle are also identified by DNA tests. Clinical problems such as freemartin calves, chromosomal defects, and hyperkalemic periodic paralysis in Quarter Horses can be diagnosed. DNA tests can be used to eliminate from breeding programs the parents of offspring with genetic deformities such as rat tail in cattle. Both favorable and unfavorable traits will be detectable as DNA technology evolves other traits . VGL is also involved in collaborative efforts aimed at the isolation and characterization of additional STR markers to be utilized in equine genomic mapping and linkage studies. The information from these studies will ultimately enable VGL to provide their clients with genetic disease diagnosis as well as genetic trait screening and selection. The Veterinary Genetics Laboratory is involved in various wildlife projects that require genetic marker testing. The Prezwalski's Horse is considered to be extinct in the wild, and the 800 animals living in zoos and reserve parks are descended from only 12 wild horses. An international cooperative breeding program aims to preserve genetic variation among the Prezwalski's Horses and minimizes in-breeding by monitoring pedigrees. The Veterinary Genetics Laboratory is engaged in testing for wildlife population studies, including DNA typing tests to characterize bighorn sheep populations for a large study that will contribute to population management. In another collaborative endeavor, the laboratory is performing DNA fingerprinting analyses of beavers in the Sacramento area to determine genetic similarities among animals. VGL is the only laboratory in the world doing blood typing for llamas and alpacas for parentage verification.

Impacts
The Veterinary Genetics Laboratory is a resource for California, the Nation, and the world-at-large, and will continue to provide an important service as even more DNA-based diagnostic techniques for domestic and wildlife species and animal diseases are developed.

Publications

No publications reported this period