Source: NORTH CAROLINA STATE UNIV submitted to NRP
BIOLOGICAL ENGINEERING APPLICATIONS OF THE ELECTRONIC NOSE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0165758
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 1999
Project End Date
Oct 1, 2004
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
NORTH CAROLINA STATE UNIV
(N/A)
RALEIGH,NC 27695
Performing Department
BIOLOGICAL & AGRICULTURAL ENGINEERING
Non Technical Summary
Characterizing and documenting livestock waste odors, improving quality evaluation in the seafood industry, and testing for food adulteration are all problems that can be addressed by the electronic nose. This project uses the electronic nose to assess livestock waste odors and food freshness and quality.
Animal Health Component
50%
Research Effort Categories
Basic
25%
Applied
50%
Developmental
25%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7110811202030%
7113520202010%
7113320202010%
7113260202010%
1333599202040%
Goals / Objectives
General Objective: This research will use electronic nose technology to study problems involving environmental odors and food safety, freshness, and quality and will improve the sensitivity and specificity of the E-nose for making appropriate measurements by improving the device itself. Specific Objectives: 1. Determine quantitative information on emission of odors from livestock operations and relate the odors to the processes which produce them so that corrective measures can be evaluated. 2. Determine quantitative information on odors emanating from food and relate the odors to product safety and freshness so that corrective measures can be applied to improve food processing techniques. 3. Determine quantitative information on odors associated with food products so that adulterants can be discovered and, if possible, identified. 4. Develop new signal processing methods to improve the sensitivity and specificity of E-nose measurements.
Project Methods
Odors from Livestock Operations: The electronic nose (E-nose) will be trained on fifteen different dilutions in factors of two of synthetic swine manure slurry. The E-nose will then be used to test samples from the NC State swine operation on Lake Wheeler Road. Other studies will look at odors from ozonated versus non-ozonated livestock buildings, from buildings with and without windbreak walls, and at the effects of dust mass levels and dust particle size on odor detection. Another experiment will involve development and evaluation of a dust trap to filter dust from air samples and to volatize odorants from the trapped dust to enable quantification of gaseous and dust-borne odors. Results from E-nose studies will be compared to results from gas chromatography to quantify odorant levels and to results from human panels. These experiments will address the first objective of the project. Food Quality: The E-nose will be used to test for product decomposition in processed crab by analyzing differences at several shelf ages. The ability of the E-nose to sort the samples by age will be determined. The experiment will be repeated 3 times, and the results will be compared to information previously obtained with spectral analysis. North Carolina has many important fresh and saltwater fish and shellfish that will be tested in a similar manner since spoilage profiles differ depending upon the food product. These experiments will address the second objective of the project. Product Adulteration: The E-nose will be trained to recognize a variety meat products, e.g. pork, beef, sheep, and chicken, and then will be used to test mixtures. When the nose is fully trained, it will be tested with unknown mixtures to determine whether meat products contain other adulterants. The same process will be used to differentiate between fish varieties, e.g. flounder, catfish, Tilapia, and to test marketed products. Other tests might include using the E-nose to detect bovine-derived materials in bovine feed or sheep-derived materials in sheep feed since this practice can lead to infections such as BSE, bovine spongiform encephalopathy (mad cow disease). The actual odor libraries that will be built, the tests that will be performed, and the timeline for accomplishing the third specific objective will depend upon available funding from commodities groups. E-nose Improvements: Initial work to improve the sensitivity and specificity of the E-nose will focus on improving methods for data analysis. The NC State E-nose currently uses principle component analysis (PCA) and/or linear discriminatory analysis (LDA) for compression of data points into a 2- or 3-dimensional array following feature extraction. Three different methods K Nearest Neighbor (KNN), Least-Mean Square (LS), and ANNs are used to classify unknown odors. Additional statistical methods, e.g. cluster analysis (CA), canonical discriminant analysis (CDA), feature weighting (FW), radial basis function (RBF) and other methods will be explored over the duration of the project. This work will address the fourth specific objective.

Progress 10/01/99 to 10/01/04

Outputs
Efforts to continue the development of e-nose technology suitable for use in monitoring livestock odors, and food product quality and safety took place. In the animal waste area, work concentrated on modifying an e-nose to make it suitable for determining basic relationships between these odors. Food product quality efforts involved the development of methods to discriminate between related products. Analyses conducted indicated that e-nose technology holds the potential for these types of determinations. However, standardization on the type of sensor technology used must be accomplished before e-nose systems can be employed to their full potential.

Impacts
Development of this technology will lead to the development of improved systems for controlling animal odors, as well as freshness detection methods for foods.

Publications

  • No publications reported this period


Progress 10/01/01 to 09/30/02

Outputs
Efforts to continue the development of e-nose technology suitable for use in monitoring livestock odors, and food product quality and safety took place. In the animal waste area, work concentrated on modifying an e-nose to make it suitable for determining basic relationships between these odors and relative humidity. This was accomplished by modifying the system's sampling sequence and adding a relative humidity sensor. Food product quality efforts involved the development of methods to discriminate between related fish varieties. The development of techniques for determining food freshness was also explored. Analyses conducted indicated that e-nose technology holds the potential for these types of determinations. However, standardization on the type of sensor technology used must be accomplished before e-nose systems can be employed to their full potential.

Impacts
Development of this technology will lead to the development of improved systems for controlling animal odors, as well as freshness detection methods for foods.

Publications

  • No publications reported this period


Progress 10/01/00 to 09/30/01

Outputs
No progress.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 10/01/99 to 09/30/00

Outputs
The NC State Electronic Nose (ENoseII) located in the Department of Biological and Agricultural Engineering is currently being used to build odor libraries so that it can be trained to recognize freshness in and safety of a variety of food products. Two main studies have been made to accomplish this goal - one focusing on seafood products (such as tilapia, tuna, and catfish), the other on ground coffee (Ethiopian, Sumatra, Colombia, and Kenya). A third study involving cooking temperature is underway on skipjack tuna. In this study, cooked samples of tuna (55 deg-C and 85 deg-C) were compared to raw samples to determine the effect of cooking on tuna aroma. Early results indicate that Discriminate Analysis was able to sort the data into the three different cooking categories. The analysis algorithms will be modified and tested on unknown samples to determine their overall robustness. The building of odor libraries is a time consuming but essential process to correctly train the ENoseII. Similar studies will be performed to expand the odor libraries. In addition, permission has been acquired to import artificial hog dust, which will be used to build an odor library for livestock operations.

Impacts
(N/A)

Publications

  • Dodd TH, Hale SA, and Blanchard SM. An aroma based method for determining the storage time of fishery products. Paper 00307313. Presented at 2000 ASAE Annual International Meeting, Milwaukee, July 2000.


Progress 10/01/98 to 09/30/99

Outputs
NEW PROJECT - NO REPORT

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 01/01/98 to 12/31/98

Outputs
The avian veterinarian who performed the surgery to implant the transmitters in the preliminary study involving six 2-week-old Arbor Acres x Arbor Acres male broilers that was described in the last progress report was unable to assist with any follow-up studies. In spite of repeated attempts, another veterinarian could not be found who was willing to work on the study. Thus, no additional studies involving more birds were possible. In addition, the student who was most familiar with the data analysis system graduated and took a job out of state before a new student could be trained. He has recently returned to NC to attend graduate school and is willing to work on the data again. A manuscript based on the preliminary study will be submitted to an appropriate peer-reviewed journal during 1999.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 01/01/97 to 12/31/97

Outputs
Data Science International transmitters capable of acquiring electrocardiograms (ECGs), temperature (T), and blood pressure (BP) were implanted subcutaneously in the coelomic cavities of six 2-week-old Arbor Acres X Arbor Acres male broilers (409+/-11 gm, mean +/- SD). The birds were too small to allow introduction of the BP transducers into their vessels so BP data were not recorded. The birds were kept in individual cages and subjected to 23 hours of light and one hour of darkness. One-min ECGs and T were recorded every 15 min and analyzed in birds 17-23 days old (920+/-28 gm at 21 days). Heart rate (HR, measured in ms intervals) and heart rate variability (HRV, the square root of one over the number of terms times the sum of the difference between each R-R interval and the average R-R interval for the period) were measured for three 10-beat periods during each ECG. Low frequency (LF, breathing) and high frequency (HF, heart rate) power spectra were determined for each 1-min ECG. No significant difference (Wilcoxon Rank Sum test) was found between light (L) and dark (D) periods for LF ECG (L: 0.42+/-0.31 Hz, N=75; D: 0.41+/-0.32, N=79), HF ECG (L: 6.55+/-0.66 Hs, N=46; D: 6.45+/-0.70, N=53), HR (L: 152.7+/-1.6 ms, N=79; D: 156.3+/-1.5, N=83), HRV (L: 3.8+/-2.2 ms, N=79; 3.4+/-2.7, N=83), or T (L: 40.5+/-1.6 deg C, N=84; D: 40.7+/-1.7, N=84). Thus, male broilers do not appear to rest during the one hour of darkness provided in typical poultry house regimens.

Impacts
(N/A)

Publications

  • EKWUEME-OKOLI, T.C., BLANCHARD, S.M., DEGERNES, L.A. and GARLICH, J.D. 1997. The effect of light and dark periods on heart rate and HRV in male broilers. Proceedings of the 19th Annual International Conference of the IEEE Engineering in Medicine and Biology Society,
  • BLANCHARD, S.M., DEWOLF, D.K., EKWUEME-OKOLI, T.C., DEGERNES, L.A. and GARLICH, J.D. 1997. Heart rate and heart rate variability in male broilers at risk for sudden death syndrome. Annals of Biomedical


Progress 01/01/95 to 12/30/95

Outputs
Two Arbor Acres X Arbor Acres broilers have been used in a pilot study to investigate the effects of temperature and diet on susceptibility to Sudden Death Syndrome and Ascites. Transmitters were implanted at 3 weeks (female) and 4 weeks of age (male) when the birds weighed 600 gm and 900 gm, respectively. A blood pressure transducer, which contains a thermistor, was placed in the jugular vein, and electrodes were placed under the skin in the right pectoral and left femoral areas in both animals. Up until the birds were 7 weeks old, one- minute electrocardiograms (ECGs) were recorded every 4 hours by a telemetry system (Dataquest IV Data Acquisition System) while heart rate, activity level, and internal temperature were measured at 15-min intervals. Room temperature was measured daily, and the birds were weighted weekly throughout the study. At 7 weeks of age, the birds were switched from a Broiler Starter Diet to one which included 220 gm/ton monensin sodium, and one- minute ECGs were recorded every hour. At 8 weeks of age, they were switched back to the Broiler Starter Diet. At 9 weeks of age, they received a new diet which included 120 gm salinomycin/ton in the starter feed. At 10 weeks of age, they were returned to the Broiler Starter Diet until the experiment was terminated when the birds were 11 weeks old. The data are being examined for arrhythmias, and heart rate variability parameters are being calculated.

Impacts
(N/A)

Publications

  • NO PUBLICATIONS REPORTED THIS PERIOD.