Source: OKLAHOMA STATE UNIVERSITY submitted to
CENTRALIZED TICK REARING
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0160810
Grant No.
(N/A)
Project No.
OKL02172
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Oct 1, 2003
Project End Date
Sep 30, 2009
Grant Year
(N/A)
Project Director
Mulder, P.
Recipient Organization
OKLAHOMA STATE UNIVERSITY
(N/A)
STILLWATER,OK 74078
Performing Department
ENTOMOLOGY & PLANT PATHOLOGY
Non Technical Summary
Tick researchers at Oklahoma State University and throughout the United States require quantities of healthy pathogen free ticks for use in studies on tick biology, physiology, molecular genetics, tick transmitted diseases and the development of novel control strategies for ticks. The OSU Tick Rearing Laboratory rears six species of ticks, ensuring that quantities of larvae, nymphs, or adults of each species are available for tick research purposes. Research continues at the laboratory to improve tick rearing and storage procedures.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
31231201130100%
Goals / Objectives
A. Modify and streamline the rearing techniques and develop the optimum storage conditions for all life stages of five tick species currently reared at the Tick Rearing Laboratory including Amblyomma americanum, A. maculatum, Dermacentor variabilis, D. andersonii and Rhiphicephalus sanquineus. B. Perfect the rearing technique for Ixodes scapularis to increase the rearing capacity by manipulating the environmental conditions under which each stage is reared or maintained between blood meals. C. Determine the role of rangeland cattle in the population dynamics of adult Ixodes scapularis in North Central Oklahoma.
Project Methods
Scientists at Oklahoma State University are national and international leaders in tick research. Much of the research conducted by these scientists requires quantities of all stages of several species of ticks, including the lone star tick, Gulf Coast tick, American dog tick, Rocky Mountain wood tick, brown dog tick and the blacklegged tick. The Tick Rearing Laboratory maintains colonies of these six species of ticks specifically for use in research projects. These ticks are extensively used by OSU researchers and tick researchers throughout the United States. Research continues at the Tick Rearing Laboratory to improve rearing techniques for each species in order to have a constant supply of healthy pathogen free ticks available for tick researchers. A limited amount of research is conducted to determine if domestic livestock are a major host of adult black-legged ticks.

Progress 10/01/03 to 09/30/09

Outputs
OUTPUTS: Centralized Tick Rearing. 10/1/2008 TO 8/10/2009 A total of 249,516 ticks were dispersed from the OSU tick rearing facility for research purposes. Six separate research units at OSU used 34,354 ticks, and 215,162 ticks were dispersed to 75 research units external to OSU. These units include federal laboratories, university laboratories, and many private laboratories that request ticks for use in research and educational projects. Approximately 81.71% of ticks dispersed were adult ticks, 17.96% were nymphal ticks, and the remaining 0.32% were engorged females or larval masses. The demand for Ixodes scapularis continues to rise, and 14,082 adult I. scapularis were dispersed. Efforts to increase production of I.scapularis continue. Research to rear the larval stages of all 6 colony species on sheep has shown limited success. Three species do not respond well to feeding on sheep, and do not return the volume of engorged larval required to meet the current demands of the tick facility. Rabbits are used to rear large quantities of these 3 tick species. Research efforts will continue to determine the most effective larval rearing techniques in order to provide the volume of ticks requested by researchers PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The mission of the Oklahoma State University Tick Rearing Facility is to raise a variety of tick species for research on ticks and tick-borne pathogens at Oklahoma State University. Ticks produced beyond the needs of OSU researchers are available for purchase on a 1st come, 1st served basis. The Tick Rearing Facility is often cited as the source of ticks in research proposals and is the main source of ticks for many research units in the United States.

Publications

  • No publications reported this period


Progress 10/01/07 to 09/30/08

Outputs
OUTPUTS: A total of 183,423 ticks were dispersed from the OSU tick rearing facility for research purposes. Eight separate research units at OSU used 12,415 ticks, and 171,008 ticks were dispersed to 71 research units external to OSU. These units include federal laboratories, university laboratories, and many private laboratories that request ticks for use in research and educational projects. Approximately 94% of ticks dispersed were adult ticks, 5% were nymphal ticks, and the remaining 1% were engorged females or larval masses. The demand for Ixodes scapularis continues to rise, and 21,555 adult I. scapularis were dispersed. Efforts to increase production of I.scapularis continue. Research to rear the larval stages of all 6 colony species on sheep has shown limited success. Three species do not respond well to feeding on sheep, and do not return the volume of engorged larval required to meet the current demands of the tick facility. Rabbits are used to rear large quantities of these 3 tick species. Research efforts will continue to determine the most effective larval rearing techniques in order to provide the volume of ticks requested by researchers. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
The OSU Tick Rearing Facility provides ticks for research projects at Oklahoma State University and throughout the United States. The Tick Rearing Facility is often cited as the source of ticks in research proposals and is the main source of ticks for many research units in the United States.

Publications

  • No publications reported this period


Progress 10/01/06 to 09/30/07

Outputs
OUTPUTS: A total of 248,762 ticks were dispersed from the OSU tick rearing facility for research purposes. Ten separate research units at OSU used 21,544 ticks, and 227,218 ticks were dispersed to 59 research units external to OSU. These units include federal laboratories, university laboratories, and many private laboratories that request ticks for use in research projects. Approximately 90% of ticks dispersed were adult ticks, 8% were nymphal ticks, and the remaining 2% were engorged females or larval masses.Efforts to increase production of I.scapularis continue. The demand for Ixodes scapularis continues to rise, and 26,661 adult I. scapularis were dispersed, which represents an increase of 3,663 adult ticks over the previous year. Research to rear the larval stages of all 6 colony species on sheep has shown limited success. Three species do not respond well to feeding on sheep, and do not return the volume of engorged larval required to meet the current demands of the tick facility. Rabbits are used to rear large quantities of these 3 tick species. Research efforts will continue to determine the most effective larval rearing techniques in order to provide the volume of ticks requested by researchers. PARTICIPANTS: Lisa Coburn Teresa Ritchie Patrick Maciula Bobby Shipman Justin Cordill Cara Hammer TARGET AUDIENCES: 59 outside research units 10 campus research units

Impacts
OSU Tick Rearing Facility provides ticks for research projects at Oklahoma State University and throughout the United States. The Tick Rearing Facility is often cited as the source of ticks in research proposals and is the main source of ticks for many research units in the United States.

Publications

  • No publications reported this period


Progress 10/01/05 to 09/30/06

Outputs
A total of 289,683 ticks were dispersed from the OSU tick rearing facility for research purposes. Seven separate research units at OSU used 59,794 ticks. 229,889 ticks were dispersed to 52 research units external to OSU. These units include federal laboratories, university laboratories, and many private laboratories that request ticks for use in research projects. Approximately 80% of ticks dispersed were adult ticks, 18% were nymphal ticks, and the remaining 2% were engorged females or larval masses. Efforts to increase production of I.scapularis continue. The demand for Ixodes scapularis continues to rise, and 22,998 I. scapularis were dispersed, which represents an increase of 8,213 adult ticks over the previous year. Research to rear the larval stages of all 6 colony species on sheep has shown limited success. Three species do not respond well to feeding on sheep, and do not return the volume of engorged larval required to meet the current demands of the tick facility. Rabbits are used to rear large quantities of these 3 tick species. Research efforts will continue to determine the most effective larval rearing techniques in order to provide the volume of ticks requested by researchers.

Impacts
The OSU Tick Rearing Facility provides ticks for research projects throughout the United States. The Tick Rearing Facility is often cited as the source of ticks in research proposals and is the main source of ticks for many research units in the United States.

Publications

  • No publications reported this period


Progress 10/01/04 to 09/30/05

Outputs
Over 167,110 adult ticks were dispersed for research purposes. Four separate research units at OSU used 30,185 adult ticks. Over 136,925 ticks were dispersed to 59 research units external to OSU. These units include federal laboratories, university laboratories, and many private laboratories that request ticks for use in research projects. Due to the accounting systems all ticks dispersed are calculated as unfed adult ticks, although larval and nymphal stages were dispersed in some instances. The colony of Ixodes scapularis is well established and the nymphal stage can now be reared on sheep. Over 14,785 Ixodes scapularis were dispersed for research purposes. Initial research to rear the larval stages of all 6 colony species on sheep has been somewhat successful with most species, the main exception being Ixodes scapularis. Larval returns using sheep as hosts are less than larval returns using rabbits as hosts. Research efforts will continue to determine the most effective larval rearing techniques in order to provide the volume of ticks requested by researchers. The animal building designated for specific tick research projects separate from the tick rearing laboratory has been used three times for three specific studies.

Impacts
The OSU Tick Rearing Laboratory provides ticks for research projects throughout the United States. The Tick Rearing Laboratory is often cited as the source of ticks in research proposals and is the main source of ticks for many research units in the United States.

Publications

  • No publications reported this period


Progress 10/01/03 to 09/30/04

Outputs
Over 265,500 adult ticks were dispersed for research purposes. Nine separate research units at OSU used over 28,000 ticks. Over 237,500 ticks were dispersed to 89 research units external to OSU. These units include federal laboratories, university laboratories and many private laboratories that use the ticks for research purposes or testing for reactions to products. Due to the accounting systems all ticks dispersed are calculated as unfed adults, although larval and nymph stages are dispersed in some instances. The colony of Ixodes scapularis is well established and the nymph stage can now be reared on sheep. Over 28,000 Ix. scapularis were dispersed for research purposes. Initial research to rear the larval stages of all species on sheep have been successful with most species, the main exception being Ix. scapularis. Research efforts will continue to develop a technique to raise all larval stages on sheep. The animal building designated for specific tick research projects separate from the tick Rearing Laboratory has been used five times for five specific studies.

Impacts
The OSU Tick Rearing Laboratory provides ticks for many research projects throughout the United States. The Tick Rearing Laboratory is often cited as the source of ticks in research proposals and is the main source of ticks for many research units in the United States.

Publications

  • No publications reported this period


Progress 10/01/02 to 09/30/03

Outputs
There were 174,819 (adult ticks) dispersed for research purposes. Six different research units at OSU used 20,000 ticks. There were also 153,819 ticks dispersed to 38 different research units outside the university. Due to the accounting system all ticks dispersed are calculated as unfed adults. Larvae, or nymphs are not considered individually in these dispersal figures. Continued improvement of rearing methods for Ix. scapularis have resulted in increased production and dispersal to research units of this economically important species. Natural Gulf Coast tick populations were monitored on the OSU Animal Science North Range during March, April, and May, 2003. Cattle from Pasture 1 East were examined weekly for 8 weeks from March 27th through May 16th. Ten control animals and 10 treated animals were counted weekly. Twenty four mls of CyLence Pour-on were applied per head to treatment animals for tick control. Twelve mls were applied to the ears and poll with disposable pipets and 12 mls applied down the back line. CyLence Pour-on provided control of Gulf Coast ticks on cattle for 4 weeks. Two treatments of CyLence Pour-on 4 weeks apart were required to achieve season long Gulf Coast tick control. Lone star adult and nymphal ticks were abundant on cattle also during this study. They were only controlled around the ears , poll, and back line with this application method and not reduced in other areas.

Impacts
The OSU Tick Rearing Facility indirectly impacts medical and veterinary research of tick-bourne diseases by supplying six species of ticks for research purposes worldwide.

Publications

  • None 2003


Progress 10/01/01 to 09/30/02

Outputs
There were 156,348 (adult ticks) dispersed for research purposes. Seven different research units at OSU used 42,344 ticks. There were also 114,004 ticks dispersed to 44 different research units outside the university. Due to the accounting system all ticks dispersed are calculated as unfed adults. Larvae, or nymphs are not considered individually in these dispersal figures. The Ixodes scapularis colony is well established which has enabled us to disperse over 15000 adults to other research units outside OSU. The animal building designated for research animals has been used six times for specific studies.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 10/01/00 to 09/30/01

Outputs
There were 124,128 (adult ticks) dispersed for research purposes. Seven different research units at OSU used 42,823 ticks. There were also 81,305 ticks dispersed to 37 different research units outside the university. Due to the accounting system all ticks dispersed are calculated as unfed adults. Larve, or nymphs are not considered individually in these dispersal figures. The Ixodes scapularis colony is well established which has enabled us to disperse over 9,000 adults to other research units outside OSU. The animal building designated for research animals has been used two times for specific studies.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 10/01/99 to 09/30/00

Outputs
There were 116,333 (adult ticks) dispersed for research purposes. Seven different research untis at OSU used 38,010 ticks. Due to the accounting system all ticks dispersed are calculated as unfed adults. Larvae, or nymphs are not considered individually in these dispersal figures. The Ixodes scapularis colony is well established which has enabled us to disperse over 4000 adults to other research units outside OSU. The animal building designated for research animals has been used four times for specific studies.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 10/01/98 to 09/30/99

Outputs
There were 64,452 (adult ticks) dispersed for research purposes. Seven different research units at OSU used 38,015 ticks. Many of these ticks were dispersed as fed adults to comply with specific studies. There were also 26,437 ticks dispersed to 22 different research units outside the university. Due to the accounting system all ticks dispersed are calculated as unfed adults. Larvae, or nymphs are not considered individually in these dispersal figures. The Ixodes scapularis colony is well established in sufficient numbers that ticks are being dispersed to other research units outside OSU. The animal building designated for research animals has been used five times for specific studies.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 10/01/97 to 09/30/98

Outputs
There were 72,379 (adult ticks) dispersed for research purposes. Seven different research units at OSU used 28,017 ticks. Many of these ticks were dispersed as fed adults to comply with specific studies. There were also 44,362 ticks dispersed to 12 different research units outside the university. Due to the new accounting system all ticks dispersed are calculated as unfed adults. Larvae or nymphs are not considered individually in these dispersal figures. The Ixodes scapularis colony has been established and ticks are now being dispersed for research. The animal building designated for research animals has been used twice for specific studies.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 10/01/95 to 09/30/96

Outputs
There were 198,000 ticks dispersed for research purposes. Six different researchunits at OSU used 167,000 of these ticks while 31,000 were dispersed to 17 different scientists outside the university. Our animal lab has been utilized 4 times to conduct specific research studies. By use of our growth chamber we were able to rear several hundred Ixodes scapularis for colony and research use. Additional studies are in progress, using the growth chamber to manipulate temperature, photoperiod and humidity to make the production of I. scapularis more efficient.

Impacts
(N/A)

Publications


    Progress 10/01/94 to 09/30/95

    Outputs
    There were 181,000 ticks dispersed for research purposes. Five different research units at OSU used 161,000 of these ticks while 22,000 were dispersed to 10 different scientists outside the university. The preparation room in the new building has been completed. It has been occupied 8 different times in the past year to conduct specific research studies. We now have a growth chamber which makes it possible to colonize ticks which have varied habitat requirements. Studies are now in progress to determine the best environmental conditions to colonize Ixodes scapularis.

    Impacts
    (N/A)

    Publications

    • None.


    Progress 10/01/93 to 09/30/94

    Outputs
    The tick rearing facility is operating very efficiently. Almost 205,000 ticks, in seven species, were reared and supplied to Oklahoma State University scientists for research purposes in 1994. An additional 51,000 ticks were supplied to scientists outside the OSU system. Eight OSU and 13 other scientists have used ticks reared in these facilities for research purposes. Inside renovations to one of buildings were initiated in 1994. When renovations to this facility are completed, scientists will be able to conduct research on the transmission of pathogens to animals by ticks in a state of the art laboratory that meets all the federal guidelines for such research.

    Impacts
    (N/A)

    Publications

    • No publications reported this period.


    Progress 10/01/92 to 09/30/93

    Outputs
    Attempts have been made to feed all ixodid tick life stages on a single host species (sheep). This was done to determine if rabbits could be eliminated as a host species, thus reducing costs in the overall rearing program. These attempts have not been successful with larval ticks, but do work well with nymphs and adults. Thusfar, little progress has been made with artificial feeding, in mass, of ixodid ticks primarily because the maintenance of these systems is difficult for the lengthy feeding period of ixodid ticks.

    Impacts
    (N/A)

    Publications

    • No publications reported this period.