DEVELOPMENT OF MULTIPLE USE BARLEY VARIETIES

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
201	1550	1040	10%
201	1550	1080	10%
202	1550	1040	10%
202	1550	1080	10%
212	1550	1040	10%
212	1550	1080	10%
701	1550	1040	10%
702	1550	1040	10%
702	1550	1080	10%
703	1550	1040	10%

Progress 06/01/02 to 09/30/07

Outputs
OUTPUTS: In the course of this project we have developed potential new varieties of barley for multiple purposes: beverage, food, forage, and fuel. Barley is the most obvious way of alleviating the burden of the Pacific Northwest wheat monoculture. Introducing cropping diversity into this mindset and agroecosystem has tremendous value. We have contributed to a fundamental understanding of the genes that drive adaptation to fall-sowing. In this era of climate change, the benefits of erosion control, water savings, and risk diversification brought by winter habit crops are timely and valuable. Furthermore, we have contributed to a an understanding of the genetics of quantitative disease resistance that will bring the benefits of public investment in agricultural research to those who need it the most. PARTICIPANTS: This project provided ample opportunities for personal fulfillment, graduate and post-graduate training and networking. Partnerships were established and nurtured with industry, growers, stakes, and stakeholders. TARGET AUDIENCES: Target audiences were farmers, scientists, non-native speakers, persons for whom barley was not familiar, and consumers of barley who did not know that barley is a key component of their favorite products. These efforts have led to a greater sensitivity to the needs of and for barley - both internal and external.

Impacts
All American citizens benefit from safe, rational, and non-invasive applications of biotechnology to agriculture. This project contributes to this greater goal via the development of better barley varieties that will yield more nutritious and valuable grain. These yields will be realized without adverse environmental and social impacts. Our goal is to produce varieties that will have unique quality profiles - making them attractive to purchasers and users. Attraction means value. Quality means better malt and malt-derived products. Quality also means better food for humans - this ancient grain can be one of the keys to deflating the obesity epidemic. Barley also has promise as a biofuel source, helping America to wean itself from the quicksands of unstable energy supplies. The genetic capacity to resist pests and environmental fluctuations will be a hallmark of our varieties, making them agents of sustainability and environmental remediation. Barley is quite simply the best crop that is also a genetic model system. By coordinating barley variety development with genetics research and instruction, barley can become a vehicle for educating the American public about the importance of agriculture, biotechnology, and science.

Publications

Limin, A., A.Corey, P. Hayes, and D. B. Fowler. 2007. Low-temperature acclimation of barley cultivars used as parents in mapping populations: response to photoperiod, vernalization and phenological development. Planta. 226.139-146
Hayes, P.M. 2007. Whats your barley? Brewers Guardian. 136.46.

Progress 01/01/06 to 12/31/06

Outputs
The primary goal of the OSU barley breeding program remains the development of six-row winter malting barley varieties that will provide the barley industry with an abundant supply of high quality barley. The greatest value in barley currently lies in malt. Thus the parallel thrusts of the program are the genetics of malting quality and the genetics of winter hardiness traits. We also have a number of focused projects such as the development of forage barley, barley for algae control, and barley for human nutrition. We are addressing America's primary objective - ensuring that barley is a competitive crop - by incorporating malting quality into high yielding winter and spring habit varieties that provide growers with a sustainable and economically viable cropping option. Our approach to solving these complex issues has been to develop molecular breeding tools based on knowledge of gene locations, effects, and interactions. The immediate benefit is that we now have very good parental stocks and promising potential varieties. The benefits will be new, high yielding malting barley varieties that represent a new, dependable, and alternative source of high quality malting barley. We have three new selections for the AMBA Pilot program in 2007.

Impacts
All American citizens will benefit from safe, rational, and non-invasive applications of biotechnology to agriculture. This project contributes to this greater goal via the development of better barley varieties that will yield more nutritious and valuable grain across more of America's bountiful lands with less input. Our goal is to produce varieties that will have unique quality profiles - making them attractive to purchasers and users. Attraction means value. Quality means better malt and malt-derived products. Quality also means better food for humans: barley can be used in so many tasty ways, and all of them are as good (or better) for you than oatmeal! Barley also has promise as a biofuel source, helping America to wean itself from dangerous, costly, and unstable suppliers of energy. The genetic capacity to resist pests and environmental fluctuations will be a hallmark of our varieties, making them agents of sustainability and environmental remediation. Barley is quite simply the best crop that is also a genetic model system. By coordinating barley variety development with genetics research and instruction, barley can become a vehicle for educating the American public about the importance of agriculture, biotechnology, and science.

Publications

Druka, A., G. Muehlbauer, I. Druka,, R..Caldo, U. Baumann, N. Rostoks, A. Schreiber, R. Wise, T. Close, A. Kleinhofs, A. Graner, A. Schulman, P. Langridge, K. Sato, P. Hayes, J. McNicol, D. Marshall, and R. Waugh. 2006. An atlas of gene expression from seed to seed through barley development. Func. Int. Genomics. 6:202-211.
Szucs, P., I. Karsai, J. von Zitzewitz, K. Meszaros, L.L.D. Cooper, Y.Q. Gu, T.H.H. Chen, P.M. Hayes, and J.S. Skinner. 2006. Positional relationships between photoperiod response QTL and photoreceptor and vernalization genes in barley. Theor. Appl. Genet. 112:1277-1285.
Richardson K.L,, M. I. Vales, J. G. Kling, C. C. Mundt, and P. M. Hayes. 2006. Pyramiding and dissecting disease resistance QTL to barley stripe rust. Theor. Appl. Genet. 113:485-495.
Yun, S.J., L. Gyenis, E. Bossolini, P.M. Hayes, I. Matus, K.P. Smith, B.J. Steffenson, R. Tuberosa and G. J. Muehlbauer. 2006. Validation of quantitative trait loci for multiple disease resistances using advanced backcross lines developed with a wild barley (Hordeum vulgare subsp. spontaneum). Crop Sci. 46: 1179-1186.
Bilgic, H., B.J. Steffenson, and P.M. Hayes. 2006. Molecular mapping of loci conferring resistance to different pathotypes of the spot blotch pathogen in barley. Phytopathology 699-708.
Rossi, C., A. Cuesta-Marcos, I. Vales, L. Gomez-Pando, G. Orjeda, R. Wise, K. Sato, K. Hori, F. Capettini, H. Vivar, X. Chen, and P. Hayes. 2006. Mapping multiple disease resistance genes using a barley mapping population evaluated in Peru, Mexico, and the USA. Mol. Breeding. 18:355-366.
Singh, J., S. Zhang, C. Chen, L.D. Cooper, P. Bregitzer, A.K. Sturbaum, P.M. Hayes, and. PG. Lemaux. 2006. Remobilization over multiple generations in barley facilitates gene tagging in large genome cereals. Plant Molecular Biology. 62:937-950.
Szucs, P., J. Skinner, I. Karsai, A.Cuesta-Marcos, K.G. Haggard, A.E. Corey, T.H.H. Chen, and P.M. Hayes. 2006. Validation of the VRN-H2/VRN-H1 epistatic model in barley reveals that intron length variation in VRN-H1 may account for a continuum of vernalization sensitivity. Mol. Genet. Genomics . (online)
Hayes, P.M. and P. Szucs. 2006. Disequilibrium and association in barley: thinking outside the glass. PNAS (USA) 49:18385-18386.

Progress 01/01/05 to 12/31/05

Outputs
The primary goal of the OSU barley breeding program remains the development of six-row winter malting barley varieties that will provide the barley industry with an abundant supply of high quality barley. The greatest value in barley currently lies in malt. Thus the parallel thrusts of the program are the genetics of malting quality and the genetics of winter hardiness traits. We also have a number of focused projects such as the development of forage barley, barley for algae control, and barley for human nutrition. We are addressing a primary need for American agriculture (ensuring that barley is a competitive crop)by incorporating malting quality into high yielding winter and spring habit varieties that provide growers with a sustainable and economically viable cropping option. Our approach to solving these complex issues has been to develop molecular breeding tools based on knowledge of gene locations, effects, and interactions. The immediate benefit is that we now have very good parental stocks and promising potential varieties. The benefits will be new, high yielding malting barley varieties that represent a new, dependable, and alternative source of high quality malting barley. We have three new selections for the AMBA Pilot program in 2006.

Impacts
The judicious application of the tools of contemporary genetics to plant improvement will strengthen the economy of rural America and the minds of bodies of all American citizens. This project contributes to this goal via the development of better barley varieties that yield more nutritious and valuable grain across more acres with less input. Our goal is to produce varieties that will have unique quality profiles making them attractive to domestic and international industry. Attraction means value. Quality means better malt and malt-derived products. Quality also means better food for humans: barley can be used in so many tasty ways, and all of them are as good (or better) for you than oatmeal! Barley also has promise as a biofuel source, helping America to wean itself from dangerous and unstable suppliers of energy. The genetic capacity to resist pests and environmental fluctuations will be a hallmark of our varieties, making them agents of sustainability and environmental remediation. Barley is quite simply the best crop plant that is also a genetic model system. By coordinating barley variety development with genetics research and instruction, barley can become a vehicle for educating the American public about the importance of agriculture, biotechnology, and science.

Publications

Fu, D., P. Szucs , L. Yan, M. Helguera, J.S. Skinner, J.von Zitzewitz , P. M. Hayes, and J. Dubcovsky. 2005. Large deletions within the VRN-1 first intron are associated with spring growth habit in barley and wheat. Mol. Gen. Genomics 273:54-65.
Karsai, I., P. Szucs, , K. Meszaros, T. Filichkin P. M. Hayes, L. Lang, and Z. Bedo. 2005. The Vrn-H2 locus is a major determinant of flowering time in a facultative winter growth habit barley (Hordeum vulgare L.) mapping population. Theor. Appl. Genet. 110:1458-1466.
Clark, S.S., P. M. Hayes, and C. A. Henson. 2005. Characterization of barley tissue-ubiquitous Beta amylase2 and effects of the single nucleotide polymorphisms on the enzymes thermostability. Crop Sci. 45:1868-1876.
Bilgic, H., B. Steffenson, and P.M. Hayes. 2005.Comprehensive genetic analyses reveal differential expression of seedling and adult plant resistance to spot blotch in populations of barley. Theor. Appl. Genet. 111:1238-1250.
Yan, L., J. von Zitzewitz, J. Skinner, P.M. Hayes, and J. Dubcovsky. 2005. Molecular characterization of the duplicated meristem identity genes HvAP1a and HvAP1b in barley. Genome 48:905-912.
von Zitzewitz, J., P. Szucs, J. Dubcovsky, L. Yan, E. Francia, N. Pecchioni, A. Casas, T.H.H. Chen, P. M. Hayes, and J. Skinner. 2005. Structural and functional characterization of barley vernalization genes. Plant Mol. Bio. 59:449-467.
Vales, M.I., C. C. Schon, F. Capettini, X. M. Chen, A. E. Corey, D. E. Mather, C. C. Mundt, K. L. Richardson, J. S. Sandoval-Islas, H. F. Utz, and P. M. Hayes. 2005. Effect of population size on the estimation of QTL: A test using resistance to barley stripe rust. Theor. Appl. Genet. 111:1260-1270.
Skinner J.S., J. von Zitzewitz, P. Szucs, L. Marquez-Cedillo, T. Filichkin, K. Amundsen, E. Stockinger, M.F. Thomashow, THH Chen, an P.M. Hayes. 2005. Structural, Functional, and Phylogenetic Characterization of a Large CBF Gene Family in Barley. Plant Molecular Biology 59:533-551.
Yun, S-Y., L. Gyenis, P.M. Hayes, I. Matus, K.P. Smith, B.J. Steffenson and G.J. Muehlbauer. 2005. Quantitative trait loci for multiple disease resistance in wild barley. Crop Sci. 45:2563-2572.

Progress 01/01/04 to 12/31/04

Outputs
The primary goal of the OSU barley breeding program is the development of six-row winter malting barley varieties that will provide the barley industry with an abundant supply of high quality barley. The greatest value in barley currently lies in malt. We also have a number of focused projects in spring barley that have as an outcome the release of varieties. We also seek to contribute to an understanding of the genetics of malting quality, and in doing so, to assist all US scientists. We are addressing one of Americas pressing agricultural priorities, ensuring that barley is a competitive crop, by incorporating malting quality into high yielding winter and spring habit varieties that provide growers with a sustainable and economically viable cropping option. The major issue in developing winter malting barley is making up for lost time. There is a nearly 100 year tradition of breeding superior spring malting barley varieties in the US and throughout the world, and multiple programs have been involved. In comparison, winter malting barley improvement is in its infancy. We have not had the opportunity to make the elite x elite crosses from which most malting barley varieties derive. Instead, we have had to develop a germplasm base that has malting quality, disease resistance, and winter hardiness. Our approach to solving these complex issues has been to develop molecular breeding tools based on knowledge of gene locations, effects, and interactions. The immediate benefit is that we now have very good parental stocks. Varieties can be expected to come from crosses of these improved parents. The benefits will be new, high yielding malting barley varieties that represent a new, dependable, and alternative source of high quality malting barley. We developed, tested, characterized, and selected winter and spring barley germplasm. We completed three years of agronomic trials that have culminated in a winter barley agronomic production package. We submitted multiple selections to the AMBA Pilot program. One selection from the 2003 crop, Kab47, passed pre-screening. Within the past year, we have had the opportunity to rate winter survival (at Pendleton), disease resistance (at Corvallis), and complete our molecular characterization of our foundation genotype: 88Ab536. The results are very exciting and should position us to make rapid progress in winter malting barley improvement. We have completed genetic characterization of our stripe rust resistance gene pyramids and demonstrated that marker assisted selection can be of great utility in rapidly developing agronomically relevant germplasm.

Impacts
The Pacific Northwest is blessed with abundant natural resources. Soil and water are two fundamental resources that must be conserved and fairly apportioned, respectively. Our new barley varieties with greater cold tolerance will be able to survive the winter, provide better ground cover and thus reduce soil erosion. In addition to soil and water quality issues, we are also addressing water apportionment issues by development of varieties that will require less irrigation. Crop diseases can be catastrophic to farmers and unhealthy for consumers. By developing barley varieties with durable broad spectrum resistance we will be able to reduce chemical use and stabilize productivity and quality. Our efforts to characterize and use genetic resources are intended to guarantee the safety and security of agriculture, which depends on genetic diversity. Barley, the oldest crop, still has a lot to offer.

Publications

Francia, E., F. Rizza, L. Cattivelli, A.M. Stanca, G. Galiba, B. Toth, P.M. Hayes, J.S. Skinner, N. Pecchioni. 2003. Two loci on chromosome 5H determine low temperature tolerance in the Nure x Tremois barley map. Theor. Appl. Genet. 108: 670-680.
L.D. Cooper, L. Marquez-Cedillo, J. Singh, A. K. Sturbaum, S. Zhang, V. Edwards, K. Johnson, A. Kleinhofs, S. Rangel, V. Carollo, P. Bregitzer, P. G. Lemaux, and P. M. Hayes. 2004. Mapping Ds insertions in barley using a sequence-based approach. Mol Gen Genomics (2004) 272: 181-193.

Progress 01/01/03 to 12/31/03

Outputs
The current iteration of this project was initiated in 2002. However, it is the latest iteration in nearly 18 years of work. The underlying premise is the same, and that premise is that profitable and sustainable agriculture is the foundation of American society. Barley is an important piece of the US agricultural puzzle. Barley is a key rotation crop in most of the Pacific Northwest and barley is a principal crop in other areas of the country. Barley has the unique advantage of being an economically important crop, as well as an ideal genetic model. Accordingly, this project provides a very special opportunity to discover, validate, and adapt the latest genetics technologies to barley improvement. The principal areas of research are winterhardiness genetics, malting quality genetics, exotic germplasm characterization, dissection of quantitative resistance, and development of molecular breeding methods. These areas of endeavor are brought to bear on the very real world problems of US farmers. The goal is to create an open pipeline from the lab to the field.

Impacts
The Pacific Northwest is blessed with abundant natural resources. Soil and water are two fundamental resources that must be conserved and fairly apportioned, respectively. Our new barley varieties with greater cold tolerance will be able to survive the winter, provide better ground cover and thus reduce soil erosion. In addition to soil and water quality issues, we are also addressing water apportionment issues by development of varieties that will require less irrigation. Crop diseases can be catastrophic to farmers and unhealthy for consumers. By developing barley varieties with durable broad spectrum resistance we will be able to reduce chemical use and stabilize productivity and quality. Our efforts to characterize and use genetic resources are intended to guarantee the safety and security of agriculture, which depends on genetic diversity. Barley, the oldest crop, still has a lot to offer.

Publications

Castro, A. X. Chen, P. M. Hayes, and M. Johnston. 2003. Pyramiding quantitative trait locus (QTL) alleles determining resistance to barley stripe rust: effects on resistance at the seedling stage. Crop Sci. 43: 651-659.
Chang, Y. J. von Zitzewitz, P.M. Hayes, and T.H.H. Chen. 2003. High frequency plant regeneration from immature embryos of an elite barley cultivar (Hordeum vulgare L. cv. Morex). Plant Cell Reports. 733-738.
Castro, A. F. Capettini, A. Corey, T. Filichkia, P.M. Hayes, A. Kleinhofs, D. Kudrna, K. Richardson, S. Sandoval-Islas, C. Rossi, and H. Vivar. 2003. Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley. Theor. Appl. Genet. 107:922-930.
Clark, S.E., P.M. Hayes, and C. Henson. 2003. Effects of single nucleotide polymorphisms in Beta-amylase 1 alleles from barley on functional properties of the enzymes. Plant Phys. and Biochem. 41:798-804.
Matus, I., A. Corey, T. Filichkin, P. M. Hayes, J. Kling, W. Powell, O. Riera-Lizarazu, K. Sato, M.I. Vales , and R. Waugh. 2003. Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. sulgare background. Genome. 46:1010-1023.
Castro, A. X. Chen,A.E. Corey, T. Filichkina, P. M. Hayes, C. Mundt, K. Richardson, S. Sandoval-Islas, and H. Vivar. 2003. Pyramidingand validation of Quantitative Trait Locus (QTL) alleles determining resistance to barley stripe rust: effects on adult plant resistance. Crop Sci. 43:2234-2239.
Francia, E., F. Rizza, L. Cattivelli, A.M. Stanca, G. Galiba, B. Toth, P.M. Hayes, J.S. Skinner, N. Pecchioni. 2003. Two loci on chromosome 5H determine low temperature tolerance in the new winter x spring (Nure x Tremois) barley map. Theor. Appl. Genet. online:1024-2003.

Progress 06/01/02 to 12/01/02

Outputs
Barley breeding is a job of national importance, with widespread impact, practical results, and profound scientific implications. In this research project, we are integrating multiple activities including gene discovery, exotic germplasm introgression, functional genomics, and old-fashioned farming. The challenge is to combine in one phenotypic package agronomic performance, disease resistance, and malting quality. We are striving to achieve this integration with a spectrum of research activities, ranging from physical mapping of key regions of the barley genome to nitrogen management in field trials to reach target levels of diastatic power. Most exciting this year are discoveries relating to transcriptional activators controlling cold tolerance and the confirmation of potentially commercial levels of malting quality in an experimental selection, STAB 113.

Impacts
Better barley will lead to better barley-based products and thus help strengthen the national economy, national resolve, and encourage debate and constructive social change. This will be achieved by increasing the productivity and profitability of farming, while providing processors and consumers with safe and abundant commodities.

Publications

Igartua, E., P.M. Hayes, W.T.B. Thomas, R. Meyer, and D.E. Mather. 2002. Genetic control of quantitative grain and malt quality traits in barley. Journal of Crop Production. 5: 131-164.
Borner, A., G. Buck-Sorlin, P.M. Hayes, S. Malyshev, and V. Korzun. 2002. Molecular mapping of major genes and quantitative trait loci determining flowering time in response to photoperiod in barley. Plant Breeding 12: 129-132.
Castro, A. X. Chen, P. M. Hayes, S. J. Knapp, R. F. Line, T. Toojinda, and H. Vivar. 2002. Coincident QTL determine seedling and adult plant resistance to stripe rust in barley. Crop Science. In press.
Castro, A. X. Chen, P. M. Hayes, and M. Johnston. 2002. Pyramiding of Quantitative Trait Locus (QTL) alleles determining resistance to barley stripe rust: effects on resistance at the seedling stage. Crop Science. In press.
Matus, I. And P.M. Hayes. 2002. Genetic diversity in three groups of barley germplasm assessed by Simple Sequence Repeats. Genome. In press.
P.M. Hayes, A. Castro, L. Marquez-Cedillo, A. Corey, C. Henson, B.L. Jones, J. Kling, D. Mather, I. Matus, C. Rossi, and K. Sato. 2002. Genetic Diversity for Quantitatively Inherited Agronomic and Malting Quality Traits. In R. Von Bothmer, H. Knupffer, T. van Hintum, and K. Sato (ed.). Diversity in Barley. Elsevier Science Publishers, Amsterdam. in press

Progress 01/01/01 to 12/31/01

Outputs
Barley breeding continues to be an important exercise with practical results and scientific implications. We are focusing on facultative growth habit winter malting varieties. The challenge is to integrate agronomic performance, disease resistance, and malting quality. Most encouraging is the fact that we have three selections in advanced malting quality testing and one selection fast-tracked for commercial scale brewing trials. The watchword is marker-assisted selection via integrated germplasm advance and gene discovery.

Impacts
Pacific Northwest growers need crop alternatives and the US barley industry needs sources of supply free of high levels of disease risk. Our approach is to develop the world's oldest crop as a new crop alternative. We will add value and substantiality by developing varieties with unique quality profiles and disease resistance.

Publications

Borem, A., D.E. Mather, D.C. Rasmusson, R.G. Fulcher, and P.M. Hayes. 1999. Mapping quantitative trait loci for starch granule traits in barley. J. of Cereal Sci. 29:153-160.
Zhu, H., G. Briceno, R. Dovel, P.M. Hayes, B.H. Liu, C.T. Liu , T. Toojinda, and S.E. Ullrich. 1999. Molecular breeding for grain yield in barley: an evaluation of QTL effects in a spring barley cross. Theor. Appl. Genet. 98:772-779.
Toojinda, T., L. Broers, X.M. Chen, P.M. Hayes, A. Kleinhofs, J. Korte, D. Kudrna, H. Leung, R.F. Line, W. Powell, L. Ramsay, Vivar, and R. Waugh. 2000. Mapping qualitative and quantitative disease resistance genes in a doubled haploid population of barley. Theor. Appl. Genet. 101:580-589.
Marquez-Cedillo, L.A., P.M. Hayes, B.L. Jones, A. Kleinhofs, W.G. Legge, B.G. Rossnagel, K. Sato, S.E. Ullrich, D.M. Wesenberg, and the NABGMP. 2000. QTL analysis of malting quality in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups. Theor. Appl. Genet. 101:173-184.
Mahfoozi, S., A. E. Limin, P. M. Hayes, P. Hucl, and D. B. Fowler. 2000. Influence of photoperiod response on the expression of cold hardiness in wheat and barley. Can. J. Plant Sci. 80:721-724.
Sato, K. T. Inukai , and P. M. Hayes. 2001. QTL analysis of resistance to the rice blast pathogen in barley (Hordeum vulgare). Theor. Appl. Genet. 102:916-920.
Van Sanford, D., J. Anderson, K.Campbell, J. Costa, P. Cregan,, C. Griffey, P. Hayes, and R. Ward. 2001. Discovery and development of molecular markers linked to Fusarium Head Blight resistance: an integrated system for wheat and barley. Crop Sci. 638-644.
Karsai, I., K. Meszaros, L. Lang, P,M. Hayes, and Z. Bedo. 2001. Multivariate analysis of traits determining adaptation in cultivated barley. Plant Breeding 120:217-222.
Marquez-Cedillo, L.A., P.M. Hayes, A. Kleinhofs, W.G. Legge, B.G. Rossnagel, K. Sato, S.E. Ullrich, D.M. Wesenberg, and the NABGMP. 2001. QTL analysis of agronomic traits in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups. Theor. Appl. Genet. 103:625-637.
Costa, J.M., S. Kramer, C. Jobet, R. Wolfe, A. Kleinhofs, D. Kudrna, A. Corey, S. McCoy, O. Riera-Lizarazu, K. Sato, T. Toojinda, I. Vales, P. Szucs, and P. M. Hayes. 2001. Molecular mapping of the Oregon Wolfe Barleys: an exceptionally polymorphic doubled-haploid population. Theor. Appl. Genet.103:415-424.
Castro, A. X. Chen, P. M. Hayes, S. J. Knapp, R. F. Line, T. Toojinda, and H. Vivar. 2002. Coincident QTL determine seedling and adult plant resistance to stripe rust in barley. Crop Science. In press.
Germplasm registrations Hayes, P.M., A.E. Corey, M. Verhoeven, M. Kolding, and W.E. Kronstad. 1995. Registration of Kold barley. Crop Sci. 35:1503.
Hayes, P.M., A.E. Corey, R. Dovel, M. Verhoeven, and W.E. Kronstad. 1995. Registration of Maranna barley. Crop Sci. 35:1504.
Hayes, P.M., A.E. Corey, R. Dovel, R. Karow, C. Mundt, K. Rhinart, and H. Vivar. 2000. Registration of Orca barley. Crop Sci. 40:849-851.
Wesenberg, D.M.., D.E. Burrup, W.M. Brown, V.R. Velasco, J.P. Hill, J.C. Whitmore, R.S. Karow, P.M. Hayes, S.E. Ullrich, and C.T. Liu. 2001. Registration of Bancroft barley. Crop Sci. 41:265-266.
P.M. Hayes, A. Castro, L. Marquez-Cedillo, A. Corey, C. Henson, B.L. Jones, J. Kling, D. Mather, I. Matus, C. Rossi, and K. Sato. 2002. Genetic Diversity for Quantitatively Inherited Agronomic and Malting Quality Traits. In R. Von Bothmer, H. Knupffer, T. van Hintum, and K. Sato (ed.). Diversity in Barley. Elsevier Science Publishers, Amsterdam. in press
P.M. Hayes, A. Corey, and J. DeNoma. Doubled haploid production in barley, using the Hordeum bulbosum technique. 2002. In. M. Maluszynski, K. Kasha , and B.P. Forster. (ed). Doubled haploid production in crop plants: a manual. FAO/IAEA (Vienna). in press.
Ullrich, S.E.. C.E. Muir, V.A. Jitkov, J.W. Burns, P.E. Reisenauer, D.M. Wesenberg, R.F. Line, X. Chen, C.T. Liu, L.D. Robertson, R.S. Karow, and P.M. Hayes. 2002. Registration of Farmington barley. Crop Sci. In press
Book Chapters Kasha, K.J., A. Kleinhofs, A. Kilian, M. Saghai Maroof, G.J. Scoles., P.M. Hayes, F.Q. Chen, X. Xia, X.Z. Li, R.M. Biyashev, D. Hoffmann, L. Dahleen, T.K. Blake, B.G. Rossnagel, B.J. Steffenson, P.L. Thomas, D.E. Falk, A. Laroche, W. Kim, and S.J. Molnar. 1995. The North American barley genome map on the cross HT and its comparison to the map on cross SM. In: K. Tsunekawi (ed.) Plant Genome and Plastome: Their Structure and Evolution. Kodansha Scientific, LTD, Tokyo, JP
Hayes, P.M., F.Q. Chen, A. Kleinhofs, A. Kilian, and D. Mather. 1996. Barley genome mapping and its applications. In: P.P. Jauhar (ed.). Methods of Genome Analysis in Plants: CRC Press, Boca Raton, USA
Hayes, P.M., F.Q. Chen, A. Corey, A. Pan, T.H.H. Chen, E. Baird, W. Powell, W. Thomas, R. Waugh, Z. Bedo, I. Karsai, T. Blake, and L. Oberthur. 1997. The Dicktoo x Morex population: a model for dissecting components of winterhardiness in barley. In: P.H. Li and T.H. Chen (ed.). Plant Cold Hardiness. Plenum Press, New York, USA.
Spaner, D. B.G. Rossnagel, W.G. Legge, G.J. Scoles, P.E. Eckstein, G.A. Penner, N.A. Tinker, K.G. Briggs, D.E. Falk, J.C. Afele, P.M. Hayes and D.E. Mather. 1999. Verification of a quantitative trait locus affecting agronomic traits in two-row barley. Crop Science 39: 248-252.
Romagosa, I., F. Han, S. Ullrich, P.M. Hayes, and D. Wesenberg. 1999. Verification of QTL through realized molecular marker-assisted selection responses in a barley cross. Mol. Breeding 5:143-152.
Zhu, H., L. Gilchrist, P. Hayes, A. Kleinhofs, D. Kudrna, Z. Liu, L. Prom, B. Steffenson, T. Toojinda, and H. Vivar. 1999. Does function follow form? QTLs for Fusarium Head Blight (FHB) resistance are coincident with QTLs for inflorescence traits and plant height in a doubled haploid population of barley. Theor. Appl. Genet. 99: 1221-1232.
Karsai, I., K. Meszaros, P. Szucs, P. M. Hayes, L. Lang, and Z. Bedo. 1999. Effects of loci determining photoperiod sensitivity (Ppd-H1 ) and vernalization response (Sh2) on agronomic traits in the Dicktoo x Morex barley mapping population. Plant Breeding 118:399-403.
Hayes, P.M., J. Cerono, H. Witsenboer, M. Kuiper, M. Zabeau, K. Sato, A. Kleinhofs, D. Kudrna, A. Kilian, M. Saghai-Maroof, D. Hoffman and the North American Barley Genome Mapping Project 1997. Characterizing and exploiting genetic diversity and quantitative traits in barley (Hordeum vulgare) using AFLP markers. JQTL http://probe.nalusda.gov:8000/otherdocs/jqtl/jqtl1997-02/
Waugh, R., N. Bonar, E. Baird, B. Thomas, A. Graner, P. Hayes, and W. Powell. 1997. Homology of AFLP products in three barley mapping populations. Mol. Gen. Genet.255:311-321.
Han, F., S.E. Ullrich, A. Kleinhofs, B.L. Jones, P.M. Hayes, and D. M. Wesenberg. Fine structure mapping of the barley chromosome 1 centromere region containing malt quality QTL. 1997. Theor. Appl. Genet. 95:903-910.
Iyamabo, O.E., and P.M. Hayes. 1995. Effects of plot type on detection of quantitative trait locus effects in barley (Hordeum vulgare L.). Plant Breeding 114: 55-60.
Iyamabo. O.E., and P.M. Hayes. 1995. Effects of selection and opportunities for recombination in doubled haploid populations of barley (Hordeum vulgare L.). Plant Breeding 114: 131-136.
Van Zee K., F.Q. Chen, P.M. Hayes, T. Close, and T.H.H. Chen. 1995. Cold specific induction of a subset of dehydrin gene family members in barley (Hordeum vulgare L.). Plant Physiol. 108:1233-1239.
Han, F., S.E. Ullrich, S. Chirat, S. Menteur, L. Jestin, A. Sarrafi, P.M. Hayes, B.L. Jones, T.K. Blake, D. Wesenberg, A. Kleinhofs, and A. Kilian. 1995. Mapping of Beta glucan content and Beta glucanase activity loci in barley grain and malt. Theor. Appl. Genet. 91:921-927.
Oziel, A., P.M. Hayes, F.Q. Chen, and B. Jones. 1996. Application of quantitative trait locus mapping to the development of winter habit malting barley. Plant Breeding. 115:43-51.
Steffenson, B.J., P.M. Hayes, and A. Kleinhofs. 1996. Genetics of seedling and adult plant resistance to net blotch (Pyrenophora teres f. teres) and spot blotch (Cochliobolus sativus) in barley. Theor. Appl. Genet. 92:552-558.
Tinker, N.A., D.E. Mather, T.K. Blake, K.G. Briggs, T.M. Choo, L. Dahleen, S.M. Dofing, , S. Ullrich, and K.J. Kasha. 1996. Loci that affect agronomic performance in two-row barley. Crop Sci. 36:1053-1062.
Hayes, P.M., D. Prehn, H. Vivar, T. Blake, A. Comeau, I. Henry, M. Johnston, B. Jones, and B. Steffenson. 1996. Multiple disease resistance loci and their relationship to agronomic and quality loci in a spring barley population. J.QTL. http://probe.nalusda.gov:8000/otherdocs/jqtl/index.html
Romagosa, I., S. Ullrich, F. Han, and P.M. Hayes. 1996. Use of the additive main effects and multiplicative interaction model in QTL mapping for adaptation in barley. Theor. Appl. Genet. 93: 30-37.
Hayes, P.M.. 1996. Barley genome mapping: new insights into the malting quality of the world's oldest crop. MBAA 33:223-225.
Karsai, I., K. Meszaros, P.M. Hayes, and Z. Bedo. 1997. Effects of chromosome 2 (2H) and 7 (5H) loci on developmental patterns in barley (Hordeum vulgare L.) under different light regimes. Theor. Appl. Genet. 94:612-618.
F. Han, I. Romagosa, S.E. Ullrich, B.L. Jones, P.M. Hayes, and D.M. Wesenberg. 1997. Molecular marker -asssited selection for malting quality traits in barely. Mol. Breeding. 3:427-437.
El Attari, A., Rebai, P.M. Hayes, G. Barrault, G. Dechamp-Guillaume, and A. Sarrafi. 1998. Potential of doubled haploid lines and localization of quantitative trait loci (QTL) for partial resistance to bacterial leaf streak (Xanthomonas campestris pv. hordei) in barley. Theor. Appl. Genet. 96:95-100.
Toojinda T., E. Baird, A. Booth, L. Broers, P. Hayes, W. Powell, W. Thomas, H. Vivar, and G. Young. 1998. Introgression of quantitative trait loci (QTLs) determining stripe rust resistance in barley: an example of marker-assisted line development. Theor. Appl. Genet. 96:123-131.
Korol, A.B., Y.I Ronin, E.Nevo, and P.M. Hayes. 1998. Multi-interval mapping of correlated trait complexes. Heredity. 80:273-284.

Progress 01/01/94 to 12/30/94

Outputs
ORE00496 We are well on our way to locating the factors that determine good and evil, in the context of barley agronomic and quality performance. Our genome mapping endeavors have progressed to the point that we are assembling the comprehensive databases on various germplasm groups that will allow for predictions regarding fixed and segregating quantitative trait loci (QTL). Doubled haploid winter barley selections that represent improvements in malt extract percentage are in advanced stages of regional testing. The emphasis is on 6-rows, and the next challenge lies in improving enzyme activity. We have released Kold, the first winter barley adapted to the Pacific Northwest that is resistant to barley stripe rust. We have used molecular marker assisted backcrossing to introgress stripe rust resistance genes into adapted spring barley backgrounds. The emphasis in the spring program will be on 2-rows.

Impacts
(N/A)

Publications

MUNDT, C.C., HAYES, P. M., and SCHON, C.C. 1994. Influence of barley mixtures on severity of scald and net blotch and on yield. Plant Pathol. 43:356-361.
CHEN, F., PREHN, C., HAYES, P. M., MULROONEY, D., COREY, A., and VIVAR, H. 1994. Mapping genes for resistance to barley stripe rust (Puccinia striiformis f. sp. hordei). Theor. Appl. Genet. 88: 215-219.
CHEN F.Q., HAYES, P. M., MULROONEY, M., and PAN, A. 1994. Identification and characterization of plant calreticulin from barley (Hordeum vulgare L.). The Plant Cell 6: 835-843.
KARSAI, I., BED, Z., and HAYES, P.M. 1994. Effect of induction medium pH and maltose concentration on in vitro androgenesis of hexaploid winter triticale andwheat. Plant Cell, Tissue, and Organ Culture. 94:49-54.
PAN, A., HAYES, P.M., CHEN, F., BLAKE, T.H., BLAKE, T. T., WRIGHT, S., KARSAI, I., and BED, Z. 1994. Genetic analysis of the components of winterhardiness in barley (Hordeum vulgare L.). Theor. Appl. Genet. 89:900-910.
CHEN, F., HAYES, P.M., MULROONEY, D. M., and PAN, A. 1994. Nucleotide sequence ofa cDNA encoding a heat-shock protein (HSP 70) from barley (Hordeum vulgare L.). Plant Physiol. 106: 815.
CHEN, F. and HAYES, P.M. 1994. Nucleotide sequence and developmental expression of duplicated genes encoding protein disulfide isomerase in barley (Hordeum vulgare L.). Plant Physiol. 106:1705-1706.

Progress 01/01/93 to 12/30/93

Outputs
We have made progress on a number of fronts: variety release, variety development, and understanding the barley genome. We have released Kold, a winter 6-row resistant to barley stripe rust and Maranna, a 6-row spring feed type best suited to irrigated environments. We have developed a number of doubled haploid winter barley lines (ORW6 - ORW9) with promising malting quality. These lines are currently in the Western Regional Winter Barley Nursery. We have located quantitative trait loci (QTLs) for agronomic, quality, physiological, and disease resistance traits in a number of genetic backgrounds.

Impacts
(N/A)

Publications

Progress 01/01/92 to 12/30/92

Outputs
We have used RFLP mapping techniques to locate genes controlling (i) resistance to barley stripe rust, race 24 (ii) cold tolerance, vernalization, and crown fructan, and (iii) agronomic and quality traits. We have produced doubled haploid winter habit lines with good malting quality and yield potential. We are releasing winter 6-row and spring 6-row varieties.

Impacts
(N/A)

Publications

Progress 01/01/91 to 12/30/91

Outputs
The focus of this effort is on developing winter habit barley varieties with superior nutritional and malting quality. Approximately 1,000 doubled haploid lines are produced each year. After one cycle of seed increase, approximately 70% of the DH lines are advanced to multiple location trials. Thereafter, lines follow conventional breeding channels. The first set of DH lines reached multiple location trials in winter 1991. It appears we have made significant progress in quality and agronomic trait improvement. We conduct supporting research in doubled haploid protocol enhancement and in the application of molecular marker assisted techniques to barley improvement.

Impacts
(N/A)

Publications

BLAKE, T., LYBECK, N. and HAYES, P.M. 1991. Good, bad, and untested ideas in RFLP and QTL analysis. Plant Breeding Abs. 61:1-7.
CHEN, F., HAYES, P.M. and RIVIN, C. 1991. Hybridization of Hordeum vulgare and Zea mays. Genome 34:603-605.
HAYES, P.M. 1991. Developing designer barley. Master Brewers Assoc. of the Americas Technical Quarterly 28-4-7.
CHEN, F. and HAYES, P.M. 1991. Effect of exogenous plant growth regulators on in vitro seed set, embryo development, and haploid production in a H. vulgare X H. bulbosum cross. Plant Cell, Tissue, and Organ Culture 26:129-184.