Source: WASHINGTON STATE UNIVERSITY submitted to NRP
EXERCISE PHYSIOLOGY/MUSCLE BIOLOGY RESEARCH
Sponsoring Institution
Cooperating Schools of Veterinary Medicine
Project Status
COMPLETE
Funding Source
STATE
Reporting Frequency
Annual
Accession No.
0151746
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 1990
Project End Date
Sep 30, 2020
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
WASHINGTON STATE UNIVERSITY
240 FRENCH ADMINISTRATION BLDG
PULLMAN,WA 99164-0001
Performing Department
Animal Health Research Center
Non Technical Summary
This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.
Animal Health Component
5%
Research Effort Categories
Basic
95%
Applied
5%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3053810102050%
3053999102050%
Knowledge Area
305 - Animal Physiological Processes;

Subject Of Investigation
3999 - Animal research, general; 3810 - Horses, ponies, and mules;

Field Of Science
1020 - Physiology;
Goals / Objectives
Develop objective criteria for evaluating exercise performance. Evaluate changes in skeletal and cardiac muscle in response to exercise; Measure performance in animals during exercise.
Project Methods
Biochemical and structural examinations of blood, muscle, and ventilated air areconducted on animals before and afrer exercise.

Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Scientists and Veterinarians. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Gain of knowledge and dissemination of information via peer reviewed publications. What do you plan to do during the next reporting period to accomplish the goals?Continue gathering publications data for next reporting period.

Impacts
What was accomplished under these goals? This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is generally detailed in publications produced during this report period.

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Fenwick AJ, Fowler DK, Wu SW, Shaffer FJ, Lindberg JEM, Kinch DC, Peters JH. 2017. Direct Anandamide Activation of TRPV1 Produces Divergent Calcium and Current Responses. Frontiers in Molecular Neuroscience 10. Fenwick AJ, Wood AM, Tanner BCW. 2017. Effects of cross-bridge compliance on the force-velocity relationship and muscle power output. Plos One 12. Ghashghaee NB, Li KL, Dong WJ. 2017. Direct interaction between troponin and myosin enhances the ATPase activity of heavy meromyosin. Biologia 72:702-708. Ghashghaee NB, Tanner BCW, Dong WJ. 2017. Functional significance of C-terminal mobile domain of cardiac troponin I. Archives of Biochemistry and Biophysics 634:38-46. Goff EE, Reindl KM, Johnson C, McClean P, Offerdahl EG, Schroeder NL, White AR. 2017. Variation in external representations as part of the classroom lecture:An investigation of virtual cell animations in introductory photosynthesis instruction*. Biochemistry and Molecular Biology Education 45:226-234. Gollapudi SK, Reda SM, Chandra M. 2017. Omecamtiv Mecarbil Abolishes Length-Mediated Increase in Guinea Pig Cardiac Myofiber Ca2+ Sensitivity. Biophysical Journal 113:880-888. Greco-Otto P, Bond S, Sides R, Kwong GPS, Bayly W, Leguillette R. 2017. Workload of horses on a water treadmill: effect of speed and water height on oxygen consumption and cardiorespiratory parameters. Bmc Veterinary Research 13. Hiroyasu S, Stimac GP, Hopkinson SB, Jones JCR. 2017. Loss of beta-PIX inhibits focal adhesion disassembly and promotes keratinocyte motility via myosin light chain activation. Journal of Cell Science 130:2329-2343. Jia PP, Chastain M, Zou Y, Her CT, Chai WH. 2017. Human MLH1 suppresses the insertion of telomeric sequences at intra-chromosomal sites in telomerase-expressing cells. Nucleic Acids Research 45:1219-1232. Jones JCR, Kam CY, Harmon RM, Woychek AV, Hopkinson SB, Green KJ. 2017. Intermediate Filaments and the Plasma Membrane. Cold Spring Harbor Perspectives in Biology 9. Kinlein SA, Shahanoor Z, Romeo RD, Karatsoreos IN. 2017. Chronic Corticosterone Treatment During Adolescence Has Significant Effects on Metabolism and Skeletal Development in Male C57BL6/N Mice. Endocrinology 158:2239-2254. Maricelli JW, Bishaw YM, Wang B, Du M, Rodgers BD. Systemic SMAD7 Gene Therapy Increases Striated Muscle Mass and Enhances Exercise Capacity in a Dose-Dependent Manner. Human Gene Therapy doi:10.1089/hum.2017.158.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Maricelli JW, Kagel DR, Bishaw YM, Nelson OL, Lin DC, Rodgers BD. 2017. Sexually dimorphic skeletal muscle and cardiac dysfunction in a mouse model of limb girdle muscular dystrophy 2i. Journal of Applied Physiology 123:1126-1138. Mertz TM, Baranovskiy AG, Wang J, Tahirov TH, Shcherbakova PV. 2017. Nucleotide selectivity defect and mutator phenotype conferred by a colon cancer-associated DNA polymerase delta mutation in human cells. Oncogene 36:4427-4433. Mickelson AV, Chandra M. 2017. Hypertrophic cardiomyopathy mutation in cardiac troponin T (R95H) attenuates length-dependent activation in guinea pig cardiac muscle fibers. American Journal of Physiology-Heart and Circulatory Physiology 313:H1180-H1189. Mickelson AV, Gollapudi SK, Chandra M. 2017. Cardiomyopathy-related mutation (A30V) in mouse cardiac troponin T divergently alters the magnitude of stretch activation in alpha- and beta-myosin heavy chain fibers. American Journal of Physiology-Heart and Circulatory Physiology 312:H141-H149. Rehwaldt JD, Rodgers BD, Lin DC. 2017. Skeletal muscle contractile properties in a novel murine model for limb girdle muscular dystrophy 2i. Journal of Applied Physiology 123:1698-1707. Suderman BL, Vasavada AN. 2017. Neck Muscle Moment Arms Obtained In-Vivo from MRI: Effect of Curved and Straight Modeled Paths. Annals of Biomedical Engineering 45:2009-2024. Tropf M, Nelson OL, Lee PM, Weng HY. 2017. Cardiac and Metabolic Variables in Obese Dogs. Journal of Veterinary Internal Medicine 31:1000-1007.


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Scientists and Veterinarians. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Gain of knowledge and dissemination of information via peer reviewed publications. What do you plan to do during the next reporting period to accomplish the goals?Continue gathering publications data for next reporting period.

Impacts
What was accomplished under these goals? This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is generally detailed in publications produced during this report period, however there are no publications to report.

Publications


    Progress 10/01/14 to 09/30/15

    Outputs
    Target Audience:Scientists and Veterinarians. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Gain of knowledge and idssemination of information via peer reviewed publications. What do you plan to do during the next reporting period to accomplish the goals?Continue gathering publications data for next reporting period.

    Impacts
    What was accomplished under these goals? This is an umbrella project for exercise physiology/muscle biology researc in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications produced during the report period.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Chandra V, Gollapudi SK, Chandra M. Rat cardiac troponin T mutation (F72L)-mediated impact on thin filament cooperativity is divergently modulated by ?- and ?-myosin heavy chain isoforms. Am J Physiol (Heart Circ Physiol). 2015 309:H1260-870. Gollapudi SK, Tardiff JC, Chandra M. The functional effect of dilated cardiomyopathy mutation (R144W) in mouse cardiac troponin T is differently affected by ?- and ?-myosin heavy chain isoforms. Am J Physiol (Heart Circ Physiol). 2015 308:H884-893. Tanner BCW, Breithaupt JJ, Awinda PO. (2015) Myosin MgADP release rate decreases at longer sarcomere length to prolong myosin attachment time in skinned rat myocardium. Am J Physiol Heart Circ Physiol. 309:H2087-97. doi: 10.1152/ajpheart.00555.2015. (1 Citations, 3.9 IF)


    Progress 10/01/13 to 09/30/14

    Outputs
    Target Audience:Scientists and Veterinarians. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Gain of knowledge and dissemination of information via peer reviewed publications. What do you plan to do during the next reporting period to accomplish the goals?Resume gathering publications data for next reporting period.

    Impacts
    What was accomplished under these goals? This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications produced during the report period, however publication data was not gathered during this reporting period.

    Publications


      Progress 01/01/13 to 09/30/13

      Outputs
      Target Audience:Scientists and veterinarians. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Gain of knowledge and dissemination of information via peer reviewed publications. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

      Impacts
      What was accomplished under these goals? This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications.

      Publications


        Progress 01/01/12 to 12/31/12

        Outputs
        OUTPUTS: This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below: PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Garikipati DK, Rodgers BD. (2012) Myostatin inhibits myosatellite cell proliferation and consequently activates differentiation: evidence for endocrine-regulated transcript processing. Journal of Endocrinology 215, 177-187
        • Garikipati DK, Rodgers BD. (2012) Myostatin stimulates myosatellite cell differentiation in a novel model system: evidence for gene subfunctionalization. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 302, R1059-R1066
        • Ford SJ, Chandra M. (2012) The effects of slow skeletal troponin I expression in the murine myocardium are influenced by development-related shifts in myosin heavy chain isoform. Journal of Physiology-London 590, 6047-6063
        • Ford SJ, Mamidi R, Jimenez J, Tardiff JC, Chandra M. (2012) Effects of R92 mutations in mouse cardiac troponin T are influenced by changes in myosin heavy chain isoform. Journal of Molecular and Cellular Cardiology 53, 542-551
        • Gollapudi SK, Mamidi R, Mallampalli SL, Chandra M. (2012) The N-Terminal Extension of Cardiac Troponin T Stabilizes the Blocked State of Cardiac Thin Filament. Biophysical Journal 103, 940-948
        • Lin DC, Hershey JD, Mattoon JS, Robbins CT. (2012) Skeletal muscles of hibernating brown bears are unusually resistant to effects of denervation. Journal of Experimental Biology 215, 2081-2087
        • Mamidi R, Gollapudi SK, Mallampalli SL, Chandra M. (2012) Alanine or aspartic acid substitutions at serine23/24 of cardiac troponin I decrease thin filament activation, with no effect on crossbridge detachment kinetics. Archives of Biochemistry and Biophysics 525, 1-8
        • Zhou ZQ, Li KL, Rieck D, Ouyang YX, Chandra M, Dong WJ. (2012) Structural Dynamics of C-domain of Cardiac Troponin I Protein in Reconstituted Thin Filament. Journal of Biological Chemistry 287, 7661-7674


        Progress 01/01/11 to 12/31/11

        Outputs
        OUTPUTS: This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below: PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Chung C, Popper B, Methawasin M, Nelson OL, Gotthardt MM, Granzier HL (2011) Importance of titin based viscosity in ventricular physiology. Journal of Molecular and Cellular Cardiology. 51(3), 428-34.
        • Suderman BL, Vasavada AN. 2012 Jan 10 Moving muscle points provide accurate curved muscle paths in a model of the cervical spine. J Biomech. 45(2):400-4. Epub 2011 Oct 26. PMID: 22033046
        • Suderman BL, Krishnamoorthy B, Vasavada AN. 2011 Mar 1 Neck muscle paths and moment arms are significantly affected by wrapping surface parameters. Comput Methods Biomech Biomed Engin.:1. [Epub ahead of print] PMID: 21416413


        Progress 01/01/10 to 12/31/10

        Outputs
        OUTPUTS: This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below: PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Ford S, Mamidi R, Dong W-J, Chandra, Campbell KB. Model representation of the force response to step-like length changes in constantly-activated cardiac muscle. J. Gen Physiol. 136: 159-177, 2010.
        • Ouyang Y, Mamidi R, Jayasundar JJ, Chandra M, Dong WJ. Structural and Kinetic Effects of PAK3 phosphorylation mimic of cTnI (cTnI-S151E) on the cTnC-cTnI interaction in the cardiac thin filament. J Mol Biol 400:1036-1045, 2010.


        Progress 01/01/09 to 12/31/09

        Outputs
        OUTPUTS: This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below: PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Chandra M, Mamidi R, Ford SJ, Hidalgo C, Witt C, Ottenheijm C, Labeit S, Granzier H. Nebulin alters crossbridge cycling kinetics and increases thin filament activation-a novel mechanism for increasing tension and reducing tension cost. J Biol Chem, 284: 30889-30896, 2009.
        • Kirk JA, MacGowan GA, Evans C, Smith SH, Warren CM, Mamidi R, Chandra M, Stewart AFR, Solaro RJ, Shroff SG. (2009) Left ventricular and myocardial function in mice expressing constitutively pseudo-phosphorylated cardiac troponin I. Circ Res. 105:1232-1239, 2009.
        • Nelson OL, Robbins CT. Cardiac function adaptations in hibernating grizzly bears (Ursus arctos horribilis). J Comp Physiol B. 2009 Nov 26.
        • Rodgers BD, Interlichia JP, Garikipati DK, Mamidi R, Chandra M, Nelson OL, Murry CE, Santana LF. Myostatin represses physiological hypertrophy of the heart and excitation-contraction coupling. J Physiol. 2009 Oct 15; 587(Pt 20):4873-86.
        • Granzier HL, Radke MH, Peng J, Westermann D, Nelson OL, Rost K, King NM, Yu Q, Tschope C, McNabb M, Larson DF, Labeit S, Gotthardt M. Truncation of titin's elastic PEVK region leads to cardiomyopathy with diastolic dysfunction. Circ Res. 2009 Sep 11; 105(6):557-64.
        • Rodgers BD, Interlichia JP, Garikipati DK, Mamidi R, Chandra M, Nelson OL, Murry CE, Santana LF. Myostatin represses physiological hypertrophy and excitation-contraction coupling of the heart. J. Physiol. (London). J Physiol, 587: 4873-4886, 2009.


        Progress 01/01/08 to 12/31/08

        Outputs
        OUTPUTS: This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below: PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Hershey JD, Robbins CT, Nelson OL, Lin DC 2008. Minimal seasonal alterations in the skeletal muscle of captive brown bears. Physiol Biochem Zool 1: 138-47. (PMID 18173360)
        • Vasavada AN, Danaraj J, Siegmund GP 2008. Head and neck anthropometry, vertebral geometry and neck strength in height-matched men and women. Journal of Biomechanics, 41:114-121.
        • Hershey JD, Robbins CT, Nelson OL, Lin D 2008. Minimal seasonal alterations in the skeletal muscle of captive brown bears. Physiological and Biochemical Zoology: PBZ. 81(2), 138-147.
        • Sampson SN, Jacobson RL, Sande RD, Susumi, CJ, Larntz KJ, Tucker RL, Bayly WM 2008. Reproducibility and repeatability of M-Mode echocardiographic measurements collected from 25 normal horses. Journal of Equine Veterinary Science. 19(1), 51-57.
        • Mehl ML, Sarkar DK, Schott HC, Brown JA, Sampson SN, Bayly WM 2008. Equine plasma beta-endorphin concentrations are affected by exercise intensity and time of day. Equine Veterinary Journal. 30, 567-569.
        • Sampson SN, Tucker RL, Bayly WM 2008. Relationship between VO2max, heart score, and echocardiographic measurements obtained at rest and immediately following maximal exercise in Thoroughbred horses. Equine Veterinary Journal. 30, 190-194.
        • Kingston JK, Sampson SN, Beard LA, Meyers KM, Sellon DC, Bayly WM 2008. The effect of supramaximal exercise on equine platelet function. Equine Veterinary Journal. 30, 181-183.
        • Campbell KB, Simpson AM, Campbell SG, Granzier HL, Slinker BK 2008. Dynamic left ventricular elastance: A model for integrating cardiac muscle contraction into ventricular pressure-volume relationships. J Appl Physiol 104: 958-975.
        • Xing J, Chinnaraj M, Zhang Z, Cheung HC, Dong WJ 2008. Structural studies of interactions between cardiac troponin I and actin in regulated thin filament using Forster resonance energy transfer. Biochemistry 47: 13383-93.
        • Robinson JM, Cheung HC, Dong W 2008. The cardiac CA2+-sensitive regulatory switch, a system in dynamic equilibrium. Biophys J. 95: 4772-89.
        • Wright JW, Mitzutani S, Harding JW 2008. Pathways involved in the transition from hypertension to hypertrophy to heart failure. Heart Failure Reviews, 13:367-375.


        Progress 01/01/07 to 12/31/07

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below.

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Chandra M, Tschirgi ML, Ford SJ, Slinker BK, Campbell BK. 2007. Interaction between myosin heavy chain and troponin isoforms modulate cardiac myofiber contractile dynamics. Am J Physiol Regul Integr Comp Physiol. 293(4):R1595-607.
        • Dong WJ, Xing J, Ouyang Y, An J, Cheung HC. Structural kinetics of cardiac troponin C mutants linked to familial hypertrophic and dilated cardiomyopathy in troponin complexes. J Biol Chem. 2007 Dec 5 [Epub ahead of print]
        • Dong WJ, Jayasundar JJ, An J, Xing J, Cheung HC. Effects of PKA phosphorylation of cardiac troponin I and strong crossbridge on conformational transitions of the N-domain of cardiac troponin C in regulated thin filaments. Biochemistry. 2007 Aug 28;46(34):9752-61. Epub 2007 Aug 3.


        Progress 01/01/06 to 12/31/06

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Granzier HL, Campbell KB. New insights in the role of cardiac myosin binding protein C as a regulator of cardiac contractility. Circ Res. 99(8):795-797, 2006.
        • Tschirgi ML, Rajapkase I, Chandra M. Functional consequence of mutation in cardiac troponin T is affected differently by myosin heavy chain isoforms. J Physiol. 574:263-273, 2006.
        • Gallon EC, Tschirgi ML, Chandra M. Differences in Myofilament calcium sensitivity in rat psoas fibers reconstituted with Troponin T isoforms containing the α and β exon. Arch Biochem Biophys. [Epub ahead of print], 2006.
        • Granzier H, Labeit S. 2006. Titin is an adjustable molecular spring. Exercise and Sports Medicine, 34, 2, 50-53.
        • Witt CC, Burkart C, Labeit D, McNabb M, Wu Y, Granzier H, Labeit S. Nebulin is a molecular ruler for skeletal muscle thin filaments. EMBO Journal. 2006 Aug 23;25(16):3843-55.
        • Gerull B, Atherton J, Geupel A, Sasse-Klaassen S, Heuser A, Frenneaux M, McNabb M, Granzier H, Labeit S, Thierfelder L. Identification of a novel frameshift mutation in the giant TTN in a large Australian family with dilated cardiomyopathy. Journal of Molecular Medicine. 2006 Jun;84(6):478-83.
        • Chandra M., Tschirgi ML, Rajapakse I, Campbell KB. Troponin T modulates the magnitude and the rate by which sarcomere length mediates the recruitment of crossbridges in cardiac muscle. Biophys. J. 90: 2867-2876, 2006.
        • Hidalgo C, Wu Y, Campbell K, Granzier H 2006. Effect of Diastolic pressure on MLC2v phosphorylation. Archives of Biochemistry and Biophysics. 2006 Aug 14; [Epub ahead of print]
        • Labeit S, Lahmers S, Burkartb C, Fong C, McNabb M, Witt S, Witt C, Labeit D, Granzier H 2006. Expression of distinct classes of titin isoforms in striated and smooth muscles by alternative splicing, and their conserved interaction with filamins. Journal of Molecular Biology, Sep 29;362(4):664-81. Epub 2006 Aug 1.
        • Henk L. Granzier K, Campbell K, 2006. New insights in the role of cMyBP-c as a regulator of cardiac contractility. Circulation Research, Oct 13;99; 8:795-7.


        Progress 01/01/05 to 12/31/05

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below.

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Campbell, K.B., Wu, Y., Simpson, A.M., Kirkpatrick, R.D., Shroff, S.G., Granzier, H.L. and Slinker, B.K. 2005.Dynamic myocardial contractile parameters from left ventricular pressure-volume measurements. Am J Physiol 289 , H114-130.
        • Chandra, M., Tschirgi, M.L., Slinker, B.K., Campbell, K,B. 2005 Troponin T regulates low-frequency cardiac muscle mechano-dynamics. Am. J. Physiol. 286: H1535-H1545.
        • Chandra, M., Tschirgi, M.L. and Tardiff, J.C. 2005. Expression of cardiac Troponin T hotspot mutants (Arg92Trp and Arg92Leu) in mouse hearts enhances myofilament calcium sensitivity. Am. J. Physiol. (Heart Circ. Physiol.) 289: H2112-H2119.
        • Witt, S.H., Witt, C.C., Granzier, H. and Labeit, S. 2005. MURF-1 and MURF2 target a specific subset of myofibrillar proteins redundantly: Towards understanding MURF-family dependent muscle turnover. Journal of Molecular Biology, 350,713-722.
        • Nagy, A., Grama, L., Huber, T., Bianco, P. , Trombitas, K., Granzier, H.L. and Kellermayer, M.S.Z. 2005. Hierarchical extensibility in the PEVK domain of skeletal-muscle titin. Biophysical Journal, 2005 Jul;89(1):329-36. Epub Apr 22.
        • Granzier, H. and Labeit, S. 2005. Titin and its associated proteins: the third myofilament system of the sarcomere. Adv Protein Chem. 71:89-119
        • Granzier, H., Wu, Y., Labeit, S., and LeWinter, M. 2005. Titin: physiological function and role in cardiomyopathy and failure. Heart Fail Rev. 2005 Sep;10(3):211-23.
        • Anderson, J.S., Hsu, A.W., Vasavada, A.N. 2005. Morphology, architecture and biomechanics of human cervical multifidus muscles. Spine, 30:4:E86-E91.


        Progress 01/01/04 to 12/31/04

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below.

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Campbell KC, Chandra M, Kirkpatrick RD, Slinker BK, Hunter WC. 2004. Interpreting cardiac muscle force-length dynamics using a novel functional model. Am. J. Physiol. 286: H1535-H1545.
        • Fukuda N, Granzier H. 2004. Role of the Giant Elastic Protein Titin in the Frank-Starling Mechanism of the Heart. Curr Vasc Pharmacol., 2, 135-139.
        • Lahmers SM, Wu Y, Call DR, Labeit S, Granzier HL. 2004 Jan 05. Developmental control of titin isoform expression and passive stiffness in fetal and neonatal myocardium. Circulation Research: Mar 5; 94(4):505-13. Epub.
        • Granzier HL Labeit S. 2004. The giant protein titin: a major player in myocardial mechanics, signaling and disease. Circulation Research. 20; 94(3):284-95.
        • Fukuda N, Wu Y, Farman G, Irving TC, Granzier H. 2004. Titin-based modulation of active tension and interfilament lattice spacing in skinned rat cardiac muscle. Pflugers Archives -European Journal of Physiology.
        • Nagueh SF, Shah G, Wu Y, Torre-Amione G, King N, Lahmers S, Witt CC, Becker K, Labeit S, Granzier H. 2004. Altered titin expression, myocardial stiffness, and left ventricular function in patients with dilated cardiomyopathy. Circulation, 110:155-162.
        • Fujita H, Labeit D, Gerull B, Labeit S, Henk L. Granzier H 2004. Titin-isoform dependent adjustment of myocardial tension by calcium. American Journal of Physiology, Heart and Circulation; 287(6):H2528-34.


        Progress 01/01/03 to 12/31/03

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below.

        Impacts
        Impact narrative has not been submitted. This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University.

        Publications

        • Gotthardt M, Nevitt M, Witt C, Strauss M, Hammer B, Richardson JA, Shelton J, Trombitas C, Wu Y, Granzier H, Labeit S, Herz J. 2003. Conditional expression of mutant M-line titins results in cardiomyopathy with altered sarcomere structure and myofibrillar signaling. Journal of Biological Chemistry;278(8):6059-65.
        • Granzier H, Wu Y, Trombitas K, Witt C, Labeit S, Bell S, LeWinter M. 2003. Variable Titin-Based Stiffness Adjustment in Heart Disease. Circulation;108:e23
        • Trombitas K, Wu Y, McNabb M, Greaser M, Kellermayer M, Labeit S, Granzier H. 2003. Molecular basis of passive stress-relaxation in human soleus fibers. Biophysical Journal ;85(5):3142-53.
        • Labeit D, Watanabe K, Witt C, Wu Y, Labeit S, Granzier H. 2003 Calcium-dependent molecular spring elements in the giant protein titin. Proc Natl Acad Sci U S A. 11;100(23):13716-21.
        • Kellermayer M, Bustamante C, Granzier H. 2003. Mechanics and structure of titin oligomers explored with atomic force microscopy. Biochimica et Biophysica Acta;1604(2):105-14.
        • Miller MK, Bang M-L, Witt CC, Labeit D, Trombitas C, Watanabe K, McElhinny AS, Granzier H, Gregorio CC, Labeit S. 2003. The Muscle Ankyrin Repeat Proteins: CARP, ankrd2/Arpp and DARP as a Family of Titin Filament Based Stress Response Molecules. J Mol Biol. 7;333(5):951-64.
        • Lin DC, Nichols TR. 2003. Parameter estimation in a crossbridge muscle model. ASME Journal of Biomechanical Engineering. 125(1): 132-140.
        • Fukuda N, Wu Y, Farman G, Irving T, Henk Granzier H. 2003 Titin isoform variance and length dependence of activation in skinned bovine cardiac muscle. J Physiol.; 553(Pt 1):147-54.


        Progress 01/01/02 to 12/31/02

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below.

        Impacts
        (N/A)

        Publications

        • Bolliger C, DeCamp CE, Stajich M, Flo GL, Martinez SA, Bennett RL, Bebchuk T. 2002. Gait analysis of dogs with hip dysplasia treated with gold bead implantation acupuncture. Vet Comp Ortho Trauma; 15:116-122.
        • Solaro RJ, Varghese J, Marian AJ, Chandra M. 2002. Molecular mechanisms of cardiac myofilament activation: modulation by pH and a troponin T mutant R92Q. Basic Res Cardiol. 97:I102-I110.
        • Yamasaki R, Wu Y, McNabb M, Greaser M, Labeit S, Granzier H. 2002. Protein kinase A phosphorylates titin's cardiac-specific N2B domain and reduces passive tension in rat cardiac myocytes. Circ Res. 90:1181-8.
        • Gerull B, Gramlich M, Atherton J, McNabb M, Trombitas K, Sasse-Klaassen S, Seidman JG, Seidman C, Granzier H, Labeit S, Frenneaux M, Thierfelder L. 2002. Mutations of TTN, encoding the giant muscle filament titin, cause familial dilated cardiomyopathy. Nat Genet. 30:201-4.
        • Gotthardt M, Hammer RE, Hubner N, Monti J, Witt CC, McNabb M, Richardson JA, Granzier H, Labeit S, Herz J. 2002. Conditional expression of mutant M-line titins results in cardiomyopathy with altered sarcomere structure. J Biol Chem.
        • Granzier H, Labeit S. 2002. Cardiac titin: an adjustable multi-functional spring. J Physiol. 541:335-42.
        • Toursel T, Stevens L, Granzier H, Mounier Y. 2002. Passive tension of rat skeletal soleus muscle fibers: effects of unloading conditions. J Appl Physiol. 92:1465-72.
        • Watanabe K, Muhle-Goll C, Kellermayer MS, Labeit S, Granzier H. 2002. Different molecular mechanics displayed by titin's constitutively and differentially expressed tandem Ig segments. J Struct Biol. 137:248-58.
        • Watanabe K, Nair P, Labeit D, Kellermayer MS, Greaser M, Labeit S, Granzier H. 2002. Molecular mechanics of cardiac titin's PEVK and N2B spring elements. J Biol Chem. 277:11549-58.
        • Wu Y, Bell SP, Trombitas K, Witt CC, Labeit S, LeWinter MM, Granzier H. 2002. Changes in titin isoform expression in pacing-induced cardiac failure give rise to increased passive muscle stiffness. Circulation. 106:1384-9.
        • Booz GW, Day JNE, Speth RC, Baker KM. 2002. Endothelin-1 inhibits the LIF receptor in cardiomyocytes by an ERK 1 and 2-dependent process. Mol Cell Biochem. 240: 39-46.
        • Vasavada, AN, Peterson, BW, Delp, SL. 2002. Three-dimensional spatial tuning of neck muscle activations in humans. Experimental Brain Research, 147:437-448.


        Progress 01/01/01 to 12/31/01

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below.

        Impacts
        (N/A)

        Publications

        • Campbell KB, Razumova MV, Kirkpatrick RD, Slinker BK. 2001. Nonlinear myofilament regulatory processes affect frequency-dependent muscle fiber stiffness. Biophys J. 81(4):2278-96.
        • Campbell KB, Razumova MV, Kirkpatrick RD, Slinker BK. 2001. Myofilament kinetics in isometric twitch dynamics. Ann Biomed Eng. 29(5):384-405.
        • Bang ML, Centner T, Fornoff F, Geach AJ, Gotthardt M, McNabb M, Witt CC, Labeit D, Gregorio CC, Granzier H, Labeit S. 2001. The complete gene sequence of titin, expression of an unusual approximately 700-kDa titin isoform, and its interaction with obscurin identify a novel Z-line to I-band linking system. Circ Res. Nov 23;89(11):1065-72.
        • Yamasaki R, Berri M, Wu Y, Trombitas K, McNabb M, Kellermayer MS, Witt C, Labeit D, Labeit S, Greaser M, Granzier H. 2001. Titin-actin interaction in mouse myocardium: passive tension modulation and its regulation by calcium/S100A1. Biophys J. Oct;81(4):2297-313.
        • Cazorla O, Wu Y, Irving TC, Granzier H. 2001. Titin-based modulation of calcium sensitivity of active tension in mouse skinned cardiac myocytes. Circ Res. May 25;88(10):1028-35.
        • Bang ML, Mudry RE, McElhinny AS, Trombitas K, Geach AJ, Yamasaki R, Sorimachi H, Granzier H, Gregorio CC, Labeit S. 2001. Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies. J Cell Biol. Apr 16;153(2):413-27.
        • Centner T, Yano J, Kimura E, McElhinny AS, Pelin K, Witt CC, Bang ML, Trombitas K, Granzier H, Gregorio CC, Sorimachi H, Labeit S. 2001. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J Mol Biol. Mar 2;306(4):717-26.
        • Kellermayer MS, Smith SB, Bustamante C, Granzier HL. 2001. Mechanical fatigue in repetitively stretched single molecules of titin. Biophys J. Feb;80(2):852-63.
        • Hudson, T.A., Bragg, J.A., Lin, D.C., and DeWeerth, S.P. 2001. An integrated circuit implementation of the Huxley sarcomere model. IEEE Transactions on Biomedical Engineering.48(12): 1471-1479.


        Progress 01/01/00 to 12/31/00

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below.

        Impacts
        (N/A)

        Publications

        • Katz LM, Bayly WM, Garber MJ, Kingston JK, Hines MT. 2000. Effects of training on maximal oxygen consumption of ponies. Amer J Vet Res 61:986-991.
        • Bell, S.P., Nyland, L., Tischler, M.D., Granzier, H., and LeWinter, M.M. 2000. Alterations in the mechanisms of diastolic suction during pacing tachycardia heart failure. Circulation Research 87(3):235-40.
        • Burattin R, Campbell KB. 2000. Physiological relevance of uniform elastic tube-models to infer descending aortic wave reflection: a problem of identifiability. Ann Biomed Eng. 28(5):512-23.
        • Razumova MV, Bukatina AE, Campbell KB. 2000. Different myofilament nearest-neighbor interactions have distinctive effects on contractile behavior. Biophys J. 78(6):3120-37.
        • Slinker BK, Wu Y, Green HW 3rd, Kirkpatrick RD, Campbell KB. 2000. Overall cardiac functional effect of positive inotropic drugs with differing effects on relaxation. J Cardiovasc Pharmacol. 36(1):1-13.
        • Trombitas, K., Freiburg, A., Greaser, M., Labeit, S., and Granzier, H. 2000. From connecting filaments to co-expression of titin isoforms. Advances in Experimental Medicine and Biology 481:405-418.
        • Trombitas, K., Redkar, A., Freiburg, A., Centner, T., Wu, Y., Labeit, S., and Granzier H. 2000. Extensibility of isoforms of cardiac titin: variation in fractional extension of molecular subsegments provides a basis for diastolic stiffness diversity. Biophysical Journal 79:3226-3234.
        • Wu, Y., Cazorla, O., Labeit, S., Granzier H. 2000. Passive stiffness of myocardium correlates with differential expression of cardiac titin isoform. Journal of Molecular and Cellular Cardiology 32:2151-2161.
        • Cazorla, O., Freiburg, A, Helmes, M., Centner, T., McNabb, M., Trombitas, K., Labeit, S., and Granzier, H. 2000. Differential expression of cardiac titin isoforms and modulation of cellular stiffness. Circulation Research 86:59-67.
        • Centner, T., Witt, C., Beckman, J., Granzier, H., Trombitas, K., Gregorio, C., and Labeit, S. 2000. Molecular tools for the study of Titin's differential expression. Advances in Experimental Medicine and Biology 481:35-53.
        • Gerber, V., M. King, D.A. Schneider, and N.E. Robinson. 2000. Tracheobronchial mucus viscoelasticity during environmental challenge in horses with recurrent airway obstruction. Equine Vet. J. 32(5):411-417.
        • Kellermayer, M., Smith, S., Bustamante, C., and Granzier, H. 2000. Mechanical manipulation of single titin molecules with laser tweezers. Advances in Experimental Medicine and Biology, 481:111-128.


        Progress 01/01/99 to 12/31/99

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below.

        Impacts
        (N/A)

        Publications

        • Helmes M, Trombitas K, Centner T, Kellermayer M, Labeit S, Linke WA, Granzier H. 1999. Mechanically driven contour-length adjustment in rat cardiac titin's unique N2B sequence: titin is an adjustable spring. Circ Res. 84(11):1339-52.
        • Ogut O, Granzier H, Jin JP. 1999. Acidic and basic troponin T isoforms in mature fast-twitch skeletal muscle and effect on contractility. Am J Physiol. 276(5 Pt 1): C1162-70.
        • Slinker BK, Wu Y, Brennan AJ, Campbell KB, Harding JW. 1999. Angiotensin IV has mixed effects on left ventricle systolic function and speeds relaxation. Cardiovasc Res. 42(3):660-9.
        • Trombitas K, Pollack GH, Greaser ML. 1999. Adaptation of a super-sensitive epitope detection technique for the immunoelectron microscopy of titin filaments in vertebrate striated muscle. J Microsc. 196 (Pt 3):299-304.
        • Burattini, R., S. Natalucci, K.B. Campbell. 1999. Viscoelasticity modulates resonance in the terminal aortic circulation. Med. Eng. Phys. 21(3): 175-185.
        • Razumova, M.V., A.E. Bukatina, K.B. Campbell. 1999. Stiffness-distortion sarcomere model for muscle simulation. Am J. Appl. Physiol. 87(5): 1861-1876.
        • Pascarel C, Brette F, Cazorla O, Guennec JY. 1999. Effects on l-type calcium current of agents interfering with the cytoskeleton of isolated guinea-pig ventricular myocytes. Exp Physiol. 84(6):1043-50.
        • Cazorla O, Vassort G, Garnier D, Le Guennec JY. 1999. Length modulation of active force in rat cardiac myocytes: is titin the sensor? J Mol Cell Cardiol. 31(6):1215-27.
        • Trombitas K, Freiburg A, Centner T, Labeit S, Granzier H. 1999. Molecular dissection of N2B cardiac Titin's extensibility. Biophys J. 77(6):3189-96.
        • Keegan RD, Greene SA, Brown JA, Weil AB, Bayly WM. 1999. Cardiopulmonary, acid-base, and blood and plasma volume effects of pre-exercise furosemide administration and post-exercise anesthesia, in horses exercised supramaximally to fatigue. Equine Vet J, Suppl. 30:174-177.
        • Greene SA, Keegan RD, Brown JA, Kingston JL, Bayly WM. 1999. Effect of furosemide on arterial acid-base, blood gases and electrolytes in horses given hypertonic saline during anesthesia immediately following supramaximal exercise to fatigue. Equine Vet J, Suppl. 30:434-437.
        • Rankin DC, Greene SA, Keegan RD, Weil AB, Schneider RK, Bayly WM. 1999. Anesthesia of horses with a combination of detomidine, zolazepam, tiletamine, and isoflurane immediately after strenuous treadmill exercise. Am J Vet Res. 60:743-748.
        • Gigante A, Chillemi C, Potter KA, Bertoni-Freddari C, Greco F. 1999. Elastic fibers of mucoskeletal tissues in bovine Marfan syndrome: A morphometric study. Journal of Orthopedic Research 17:624-628. Burattini, R., K.B. Campbell. 1999. Assessment of aortic pressure power components and their link to overall elastic and resistive arterial properties. Med. Biol. Eng. Comput. 37(3): 366-376.
        • Rankin DC, Greene SA, Keegan RD, Weil AB, Schneider RK, Bayly WM. 1999. Anesthesia of horses with a combination of detomidine, zolazepam, tiletamine, and isoflurane immediately after strenuous treadmill exercise. Am J Vet Res; 60:743-748.


        Progress 01/01/98 to 12/31/98

        Outputs
        This is an umbrella project for exercise physiology/muscle biology research in the College of Veterinary Medicine at Washington State University. Progress in this project is detailed in the publications listed below.

        Impacts
        (N/A)

        Publications

        • Bukatina, A.E., R.D. Kirkpatrick, K.B. Campbell. 1998. Dethiophalloidin increases Ca2+ responsiveness of skinned cardiac muscle. J. Musc. Res. & Cell Motil. 19: 515-523.
        • Trombitas, K., M. Greaser, S. Labet, J.-P. Jin, M. Kellermayer, M. Helmes and H. Granzier. 1998. Titin extensibility in situ: entropic elasticity of permanently folded and permanently unfolded molecular segment. Journal of Cell Biology. 140(4): 1-7.
        • Kellermayer, M., S. Smith, C. Bustamante and H. Granzier. 1998. Complete unfolding of the titin molecule under external force. J. Struct. Biol. 122: 197-205.
        • Trombitas, K., M. Greaser, G. French and H. Granzier. 1998. PEVK extension of human soleus muscle revealed by immunolabeling with the anti-titin antibody g100. J. Struct. Biol. 122: 188-196.
        • Linke, W., and Granzier, H. 1998. New insights in titin elasticity: a spring tale. Biophys. J. 75: 2613-2614.
        • Campbell, K.B., Y. Wu, R.D. Kirkpatrick and B.K. Slinker. 1998. Myocardial contractile depression from high-frequency vibration is not due to increased cross-bridge breakage. Am. J. Physiol. (Heart Circ. Physiol.) 274: H1141-H1151.


        Progress 01/01/97 to 12/31/97

        Outputs
        Progress in this project is detailed in the publications listed below.

        Impacts
        (N/A)

        Publications

        • SMITH JM, MEYERS KM, BARBEE DD, SCHOTT H, BAYLY WM. 1997. Plasma von Willebrand factor in Thoroughbreds in response to high-intensity treadmill exercise. AJVR. 58(1):71-76.
        • RATZLAFF MH, HYDE M, HUTTON D, RATHGEBER R, BALCH O. 1997. Interrelationships between moisture content of the track, dynamic properties of the track and the locomotor forces exerted by galloping horses. J. Equine Vet. Sci. 17(1):35-42.
        • BAYLY WM, SLOCOMBE RF. 1997. Evaluation of air-flow mechanics in a model of obstructive upper airway disease under conditions simulating exercise. Res Vet Sci 62:205-211.
        • SCHOTT HC, MCGLADE KS, MOLANDER HA, LEROUX AJ, HINES MT. 1997. Body weight, fluid, electrolyte, and hormonal changes in horses competing in 50- and 100-mile endurance rides. American Journal of Veterinary Research 58:303-309.


        Progress 01/01/96 to 12/30/96

        Outputs
        Progress in this project is detailed in the publications listed below.

        Impacts
        (N/A)

        Publications

        • SLINKER, B.K., R.L. STEPHENS, S.A. FISHER AND Q. YANG. 1996. Immediate-early gene responses to different cardiac loads in the ejecting rabbit left ventricle.J. Mol. Cell. Cardiol. 28: 1565-1574. Bharati S, Cantor GH, Leach JB, Lev M: The cardiac conduction system in sudden death in Alaskan sled dogs during the Iditarod race and/or during training. Pacing and Clinical Electrophysiology (In press). Bayly W, Andrea R, Smith B, Stenslie J, Bergsma G: 1996. Thyroid concentrations in racing Thoroughbreds. Pferdeheilkunde 12:534-438.
        • GRANZIER, H. L., M. HELMES AND K. TROMBITS. 1996. Nonuniform elasticity of titin in cardiac myocytes: A study using immunoelectron microscopy and cellular mechanics. Biophys. J. 70: 430-443.
        • KELLERMAYER, M. AND H.L. GRANZIER. 1996. Elastic properties of single titin molecules made visible through fluorescent F-actin binding. Biochem. and Biophys. Res. Comm. 221: 491-497.


        Progress 01/01/95 to 12/30/95

        Outputs
        Progress in this project is detailed in the publications listed below.

        Impacts
        (N/A)

        Publications


          Progress 01/01/94 to 12/30/94

          Outputs
          Progress in this project is detailed in the publications listed below.

          Impacts
          (N/A)

          Publications


            Progress 07/01/90 to 06/30/94

            Outputs
            Objectives: Develop objective criteria for evaluating exercise performance, evaluate changes in skeletal & cardiac muscle in response to exercise, measure performance in animals during exercise. Approach: Biochemical & structural examinations of blood, muscle, & ventilated air are conducted on animals before & after exercise. Progress towards reaching these objectives is detailed in the publications.

            Impacts
            (N/A)

            Publications

            • NO PUBLICATIONS REPORTED THIS PERIOD.


            Progress 01/01/92 to 12/30/92

            Outputs
            Objectives: Develop objective criteria for evaluating exercise performance, evaluate changes in skeletal & cardiac muscle in response to exercise, measure performance in animals during exercise. Approach: Biochemical & structural examinations of blood, muscle, & ventilated air are conducted on animals before & after exercise. Progress towards reaching these objectives is detailed in the publications listed below.

            Impacts
            (N/A)

            Publications

            • WILSON PD, RATZLAFF MH, GRANT GD, HYDE ML, BALCH OK. 1992. The effects of a compressible plastic shoe-the Seattle Shoe-on the kinematics of the strides of galloping thoroughbred horses. J Equ Vet Sci 12:374-381.
            • BALCH OK, RATZLAFF MH, HYDE ML, WHITE KK. 1992. Locomotor effects of hoof angle and mediolateral balance of horses exercising on a high-speed treadmill: Preliminary results. Proc 37th jAnn Conv Am Assoc Equ Pract 687-705.
            • BAYLY WM, SCHOTT HC, SEYMOUR KR, SLOCOMBE RF. 1992. Some comparative aspects of ventilatory responses to exercise. Proc 1st Intnl Camel Symp 229-232.


            Progress 01/01/91 to 12/30/91

            Outputs
            Objectives: Develop objective criteria for evaluating exercise performance, evaluate changes in skeletal & cardiac muscle in response to exercise, measure performance in animals during exercise. Approach: Biochemical & structural examinations of blood, muscle, & ventilated air are conducted on animals before & after exercise. Progress towards reaching these objectives is detailed in the publications listed below.

            Impacts
            (N/A)

            Publications

            • MAJOR MD, GRANT BD, WHITE KK, RATZLAFF MH, GALLINA AM, CRAWLEY GR. 1991. Suspensory apparatus prosthesis in the horse. 1: In vitro mechanical properties. J Vet Surg (in-press).
            • MAJOR MD, GRANT BD, WHITE KK, RATZLAFF MH, GALLINA AM, DONATI RM. 1991. Suspensory apparatus prosthesis in the horse. 2: In vivo evaluation in a suspensory apparatus disruption model. J Vet Surg (In press).


            Progress 12/01/89 to 12/30/90

            Outputs
            Objectives: Develop objective criteria for evaluating exercise performance, evaluate changes in skeletal & cardiac muscle in response to exercise, measure performance in animals during exercise. Approach: Biochemical & structural examinations of blood, muscle, & ventilated air are conducted on animals before & after exercise. Progress towards reaching these objectives is detailed in the publications listed below.

            Impacts
            (N/A)

            Publications

            • GOLLNICK, P.D., BERTOCCI, L.A., KELSO, T.B., WITT, E.H., HODGSON, D.R. 1990. The effect of high intensity exercise on the respiratory capacity of skeletal muscle. Pflugers Arch 415:407-413.
            • ROSE, R.J., HODGSON, D.R., BAYLY, W.M., GOLLNICK, P.D. 1990. VO2max and VO2 and VC2 kinetics in the horse: Comparison of exercise protocols. in-press.
            • ROSE, R.J., HODGSON, D.R., KELSO, T.B., MCCUTCHEON, L.J., BAYLY, W.M., GOLLNICK, P.D. 1990. Effects of acetazolamide on metabolic & respiratory responses to exercise at VO2 max. J Appl Physiol 68:617-626.