Source: OKLAHOMA STATE UNIVERSITY submitted to
PATHOGENESIS, DIAGNOSIS, TREATMENT, PREVENTION AND CONTROL OF LIVESTOCK DISEASES
Sponsoring Institution
State Agricultural Experiment Station
Project Status
COMPLETE
Funding Source
STATE
Reporting Frequency
Annual
Accession No.
0136005
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 1988
Project End Date
Sep 30, 2023
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
OKLAHOMA STATE UNIVERSITY
(N/A)
STILLWATER,OK 74078
Performing Department
Veterinary Medicine
Non Technical Summary
(N/A)
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3113810111050%
3113899111050%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3899 - Other animals, general; 3810 - Horses, ponies, and mules;

Field Of Science
1110 - Parasitology;
Goals / Objectives
To coordinate the conduct and reporting of short-term "seed" grants awarded to veterinary investigators to explore novel approaches to the health problems of livestock. It is intended that the results of these small, short-term grants will lead to more extensive research in areas that require preliminary data to justify highly creative approaches to animal health and disease.
Project Methods
The Associate Dean for Research, an Assistant Director of the Station, will be responsible for the management of research, for appropriate peer review, for disbursement of state and private funds, for the reporting of results and pursuit of extensive projects based on the preliminary data.

Progress 10/01/20 to 09/30/21

Outputs
Target Audience:This project covers agricultural and biomedical research funded by sources other than USDA that contributes to the total research capacity of the OSU College of Veterinary Medicine. We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects came from Federal, State, and private funding sources, including biological and pharmaceutical corporations. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?These research opportunities provide training for post-docs, graduate students, DVM trainees in clinical and pathology residency programs, and veterinary students during the summer and in a Research Elective course during the fall and spring semesters. How have the results been disseminated to communities of interest?Peer-reviewed publications What do you plan to do during the next reporting period to accomplish the goals?We will continue to pursue the goals outlined. Additional short term seed grants will be awarded for the next period.

Impacts
What was accomplished under these goals? To coordinate the conduct and reporting of short-term "seed" grants awarded to veterinary investigators to explore novel approaches to the health problems of livestock. It is intended that the results of these small, short-term grants will lead to more extensive research in areas that require preliminary data to justify highly creative approaches to animal health and disease. Three short-term seed awards funded in 2019. Work anticipated occurring since March has been disrupted by the COVID -19 event. 1. Influenza D pathogenesis and host immune responses in calves under single- and dual-infection. 2. Construction of a bRSV infectious clone and establishment of an experimental infection model of bRSV in neonatal calves. 3. Prevalence of anthelmintic resistance in Oklahoma beef cattle herds.

Publications


    Progress 10/01/19 to 09/30/20

    Outputs
    Target Audience:This project covers agricultural and biomedical research funded by sources other than USDA that contributes to the total research capacity of the OSU College of Veterinary Medicine. We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects came from Federal, State, and private funding sources, including biological and pharmaceutical corporations. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?These research opportunities provide training for post-docs, graduate students, DVM trainees in clinical and pathology residency programs, and veterinary students during the summer and in a Research Elective course during the fall and spring semesters. How have the results been disseminated to communities of interest?Peer-reviewed publications What do you plan to do during the next reporting period to accomplish the goals?We will continue to pursue the goals outlined. Additional short term seed grants will be awarded for the next period.

    Impacts
    What was accomplished under these goals? Three short-term seed awards funded in 2019. Work anticipated occurring since March has been disrupted by the COVID -19 event. 1. Influenza D pathogenesis and host immune responses in calves under single- and dual-infection. 2. Construction of a bRSV infectious clone and establishment of an experimental infection model ofbRSV in neonatal calves. 3. Prevalence of anthelmintic resistance in Oklahoma beef cattle herds. We expect to award new short-term seed funds in 2020, however, the cycle has been disrupted by the COVID -19 event. We are on track to make new awards before the end of October 2020.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Allen K, Little S, Petersen M, Gruntmeir J, Barrett A, Herrin B, Starkey L, Sun F, Guerino F. Evaluation of oral fluralaner (Bravecto�) for efficacy against nymphs of Amblyomma americanum and Rhipicephalus sanguineus (sensu lato). Parasit Vectors. 2020 Jun 18;13(1):315. doi: 10.1186/s13071-020-04179-y. PMID: 32552774; PMCID: PMC7302130.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Awasthi S, Rahman N, Rui B, Kumar G, Awasthi V, Breshears M, Kosanke S. Lung and general health effects of Toll-like receptor-4 (TLR4)-interacting SPA4 peptide. BMC Pulm Med. 2020 Jun 23;20(1):179. doi: 10.1186/s12890-020-01187-7. PMID: 32576172; PMCID: PMC7310322.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Ayalew S, Murdock BK, Snider TA, Confer AW. Mannheimia haemolytica IgA- specific proteases. Vet Microbiol. 2019 Dec;239:108487. doi: 10.1016/j.vetmic.2019.108487. Epub 2019 Nov 4. PMID: 31767097.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Balasubramanian P, Asirvatham-Jeyaraj N, Monteiro R, Sivasubramanian MK, Hall D, Subramanian M. Obesity-induced sympathoexcitation is associated with Nrf2 dysfunction in the rostral ventrolateral medulla. Am J Physiol Regul Integr Comp Physiol. 2020 Feb 1;318(2):R435-R444. doi: 10.1152/ajpregu.00206.2019. Epub 2019 Dec 11. PMID: 31823672; PMCID: PMC7052598.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Campolo A, Frantz MW, de Laat MA, Hartson SD, Furr MO, Lacombe VA. Differential Proteomic Expression of Equine Cardiac and Lamellar Tissue During Insulin-Induced Laminitis. Front Vet Sci. 2020 Jun 12;7:308. doi: 10.3389/fvets.2020.00308. PMID: 32596266; PMCID: PMC7303262.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Chirkova T, Ha B, Rimawi BH, Oomens AGP, Hartert TV, Anderson LJ. In vitro model for the assessment of human immune responses to subunit RSV vaccines. PLoS One. 2020 Mar 19;15(3):e0229660. doi: 10.1371/journal.pone.0229660. PMID: 32191728; PMCID: PMC7081972.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Creevy KE, Grady J, Little SE, Moore GE, Strickler BG, Thompson S, Webb JA. 2019 AAHA Canine Life Stage Guidelines. J Am Anim Hosp Assoc. 2019 Nov/Dec;55(6):267-290. doi: 10.5326/JAAHA-MS-6999. PMID: 31622127.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Derakhshan T, Bhowmick R, Meinkoth JH, Ritchey JW, Gappa-Fahlenkamp H. Human Mast Cell Development from Hematopoietic Stem Cells in a Connective Tissue- Equivalent Model. Tissue Eng Part A. 2019 Nov;25(21-22):1564-1574. doi: 10.1089/ten.TEA.2018.0347. Epub 2019 Aug 9. PMID: 30896346.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Dewage BG, Little S, Payton M, Beall M, Braff J, Szlosek D, Buch J, Knupp A. Trends in canine seroprevalence to Borrelia burgdorferi and Anaplasma spp. in the eastern USA, 2010-2017. Parasit Vectors. 2019 Oct 14;12(1):476. doi: 10.1186/s13071-019-3735-x. PMID: 31610803; PMCID: PMC6791011.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Di Girolamo N, Meursinge Reynders R. Characteristics of scientific articles on COVID-19 published during the initial 3 months of the pandemic. Scientometrics. 2020 Jul 24:1-18. doi: 10.1007/s11192-020-03632-0. Epub ahead of print. PMID: 32836530; PMCID: PMC7380499.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: d'Offay JM, Fulton RW, Eberle R, Dubovi EJ, Chase CCL. Complete genome sequence of bovine herpesvirus type 1.1 (BoHV-1.1) Los Angeles (LA) strain and its genotypic relationship to BoHV-1.1 Cooper and more recently isolated wild- type field strains. Arch Virol. 2019 Nov;164(11):2843-2848. doi: 10.1007/s00705-019-04398-4. Epub 2019 Sep 7. PMID: 31494777.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ferencz B, Condac E, Poudel N, Munteanu MC, Sivasami P, Choudhury B, Naidu NN, Zhang F, Breshears M, Linhardt RJ, Hinsdale ME. Xylosyltransferase 2 deficiency and organ homeostasis. Glycoconj J. 2020 Sep 23. doi: 10.1007/s10719-020-09945-9. Epub ahead of print. PMID: 32965647.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Fulton RW, Cook BJ, Payton ME, Burge LJ, Step DL. Immune response to bovine viral diarrhea virus (BVDV) vaccines detecting antibodies to BVDV subtypes 1a, 1b, 2a, and 2c. Vaccine. 2020 May 19;38(24):4032-4037. doi: 10.1016/j.vaccine.2020.03.058. Epub 2020 Apr 24. PMID: 32336600.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Fulton RW. Viruses in Bovine Respiratory Disease in North America: Knowledge Advances Using Genomic Testing. Vet Clin North Am Food Anim Pract. 2020 Jul;36(2):321-332. doi: 10.1016/j.cvfa.2020.02.004. PMID: 32451028; PMCID: PMC7244414.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Gorbet MJ, Ranjan A. Cancer immunotherapy with immunoadjuvants, nanoparticles, and checkpoint inhibitors: Recent progress and challenges in treatment and tracking response to immunotherapy. Pharmacol Ther. 2020 Mar;207:107456. doi: 10.1016/j.pharmthera.2019.107456. Epub 2019 Dec 19. PMID: 31863820; PMCID: PMC7039751.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Huang C, Liang Y, Zeng X, Yang X, Xu D, Gou X, Sathiaseelan R, Senavirathna LK, Wang P, Liu L. Long Noncoding RNA FENDRR Exhibits Antifibrotic Activity in Pulmonary Fibrosis. Am J Respir Cell Mol Biol. 2020 Apr;62(4):440-453. doi: 10.1165/rcmb.2018-0293OC. PMID: 31697569; PMCID: PMC7110975.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Kumar P, Agarwal PK, Cuneo MJ. On the Case of the Misplaced Hydrogens. Chembiochem. 2020 Jul 24. doi: 10.1002/cbic.202000376. Epub ahead of print. PMID: 32706524.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Li Y, Huang X, Guo F, Lei T, Li S, Monaghan-Nichols P, Jiang Z, Xin HB, Fu M. TRIM65 E3 ligase targets VCAM-1 degradation to limit LPS-induced lung inflammation. J Mol Cell Biol. 2020 Apr 24;12(3):190-201. doi: 10.1093/jmcb/mjz077. PMID: 31310649; PMCID: PMC7181722.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Maria Z, Campolo AR, Scherlag BJ, Ritchey JW, Lacombe VA. Insulin Treatment Reduces Susceptibility to Atrial Fibrillation in Type 1 Diabetic Mice. Front Cardiovasc Med. 2020 Aug 12;7:134. doi: 10.3389/fcvm.2020.00134. PMID: 32903422; PMCID: PMC7434932.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Maxwell LK, Black DH, Wright GE, Breshears MA, Eberle R. Effective Prophylactic Therapy for Exposure to Monkey B Virus (Macacine alphaherpesvirus). Comp Med. 2020 Feb 1;70(1):56-66. doi: 10.30802/AALAS- CM-18-000134. Epub 2019 Dec 6. PMID: 31810502; PMCID: PMC7024771.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Munteanu MC, Huang C, Liang Y, Sathiaseelan R, Zeng X, Liu L. Long non- coding RNA FENDRR regulates IFN?-induced M1 phenotype in macrophages. Sci Rep. 2020 Aug 13;10(1):13672. doi: 10.1038/s41598-020-70633-7. PMID: 32792604; PMCID: PMC7426844.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Nagamori Y, Hall Sedlak R, DeRosa A, Pullins A, Cree T, Loenser M, Larson BS, Smith RB, Goldstein R. Evaluation of the VETSCAN IMAGYST: an in-clinic canine and feline fecal parasite detection system integrated with a deep learning algorithm. Parasit Vectors. 2020 Jul 11;13(1):346. doi: 10.1186/s13071-020-04215-x. PMID: 32653042; PMCID: PMC7353785.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Narasaraju T, Tang BM, Herrmann M, Muller S, Chow VTK, Radic M. Neutrophilia and NETopathy as Key Pathologic Drivers of Progressive Lung Impairment in Patients With COVID-19. Front Pharmacol. 2020 Jun 5;11:870. doi: 10.3389/fphar.2020.00870. PMID: 32581816; PMCID: PMC7291833.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Narasaraju T. Histopathologic Changes and SARS-CoV-2 Immunostaining in the Lung of a Patient With COVID-19. Ann Intern Med. 2020 Aug 18;173(4):323-324. doi: 10.7326/L20-0894. PMID: 32805179. Norton E, Schultz N, Geor R, McFarlane D, Mickelson J, McCue M. Genome-Wide Association Analyses of Equine Metabolic Syndrome Phenotypes in Welsh Ponies and Morgan Horses. Genes (Basel). 2019 Nov 6;10(11):893. doi: 10.3390/genes10110893. PMID: 31698676; PMCID: PMC6895807.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Patil G, Xu L, Wu Y, Song K, Hao W, Hua F, Wang L, Li S. TRIM41-Mediated Ubiquitination of Nucleoprotein Limits Vesicular Stomatitis Virus Infection. Viruses. 2020 Jan 22;12(2):131. doi: 10.3390/v12020131. PMID: 31979016; PMCID: PMC7077221.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Piao D, Sypniewski LA, Dugat D, Bailey C, Burba DJ, DeTaboada L. Transcutaneous transmission of photobiomodulation light to the spinal canal of dog as measured from cadaver dogs using a multi-channel intra-spinal probe. Lasers Med Sci. 2019 Oct;34(8):1645-1654. doi: 10.1007/s10103-019-02761-0. Epub 2019 Mar 16. PMID: 30879228.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Pulavendran S, Prasanthi M, Ramachandran A, Grant R, Snider TA, Chow VTK, Malayer JR, Teluguakula N. Production of Neutrophil Extracellular Traps Contributes to the Pathogenesis of Francisella tularemia. Front Immunol. 2020 Apr 24;11:679. doi: 10.3389/fimmu.2020.00679. PMID: 32391009; PMCID: PMC7193117.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ridpath JF, Fulton RW, Bauermann FV, Falkenberg SM, Welch J, Confer AW. Sequential exposure to bovine viral diarrhea virus and bovine coronavirus results in increased respiratory disease lesions: clinical, immunologic, pathologic, and immunohistochemical findings. J Vet Diagn Invest. 2020 Jul;32(4):513-526. doi: 10.1177/1040638720918561. Epub 2020 Jun 2. PMID: 32484424; PMCID: PMC7438645.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Pulavendran S, Rudd JM, Maram P, Thomas PG, Akhilesh R, Malayer JR, Chow VTK, Teluguakula N. Combination Therapy Targeting Platelet Activation and Virus Replication Protects Mice against Lethal Influenza Pneumonia. Am J Respir Cell Mol Biol. 2019 Dec;61(6):689-701. doi: 10.1165/rcmb.2018-0196OC. PMID: 31070937; PMCID: PMC6890408.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Rico AB, Wang Z, Olson AT, Linville AC, Bullard BL, Weaver EA, Jones C, Wiebe MS. The Vaccinia Virus (VACV) B1 and Cellular VRK2 Kinases Promote VACV Replication Factory Formation through Phosphorylation-Dependent Inhibition of VACV B12. J Virol. 2019 Sep 30;93(20):e00855-19. doi: 10.1128/JVI.00855-19. PMID: 31341052; PMCID: PMC6798115.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Roy MM, Norton EM, Rendahl AK, Schultz NE, McFarlane D, Geor RJ, Mickelson JR, McCue ME. Assessment of the FAM174A 11G allele as a risk allele for equine metabolic syndrome. Anim Genet. 2020 Aug;51(4):607-610. doi: 10.1111/age.12952. Epub 2020 May 15. PMID: 32412131.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Silva AT, Rouf F, Semola OA, Payton ME, Lovern PC. Placental growth factor levels in quadriceps muscle are reduced by a Western diet in association with advanced glycation end products. Am J Physiol Heart Circ Physiol. 2019 Oct 1;317(4):H851-H866. doi: 10.1152/ajpheart.00511.2018. Epub 2019 Aug 9. PMID: 31397166; PMCID: PMC6843019.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Saleh MN, Sundstrom KD, Duncan KT, Ientile MM, Jordy J, Ghosh P, Little SE. Show us your ticks: a survey of ticks infesting dogs and cats across the USA. Parasit Vectors. 2019 Dec 19;12(1):595. doi: 10.1186/s13071-019-3847-3. PMID: 31856893; PMCID: PMC6923977.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Senavirathna LK, Huang C, Pushparaj S, Xu D, Liu L. Hypoxia and transforming growth factor ?1 regulation of long non-coding RNA transcriptomes in human pulmonary fibroblasts. Physiol Rep. 2020 Jan;8(1):e14343. doi: 10.14814/phy2.14343. PMID: 31925944; PMCID: PMC6954122.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Sethuraman SN, Singh MP, Patil G, Li S, Fiering S, Hoopes PJ, Guha C, Malayer J, Ranjan A. Novel calreticulin-nanoparticle in combination with focused ultrasound induces immunogenic cell death in melanoma to enhance antitumor immunity. Theranostics. 2020 Feb 10;10(8):3397-3412. doi: 10.7150/thno.42243. PMID: 32206098; PMCID: PMC7069083.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Singh MP, Sethuraman SN, Ritchey J, Fiering S, Guha C, Malayer J, Ranjan A. In-situ vaccination using focused ultrasound heating and anti-CD-40 agonistic antibody enhances T-cell mediated local and abscopal effects in murine melanoma. Int J Hyperthermia. 2019 Nov;36(sup1):64-73. doi: 10.1080/02656736.2019.1663280. PMID: 31795832; PMCID: PMC6897315.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Taylor JD, Gilliam JN, Mourer G, Stansberry C. Comparison of effects of four weaning methods on health and performance of beef calves. Animal. 2020 Jan;14(1):161-170. doi: 10.1017/S1751731119001228. Epub 2019 Jun 17. PMID: 31203825.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Young JM, Schoonover MJ, Kembel SL, Taylor JD, Bauck AG, Gilliam LL. Efficacy of orally administered gabapentin in horses with chronic thoracic limb lameness. Vet Anaesth Analg. 2020 Mar;47(2):259-266. doi: 10.1016/j.vaa.2019.11.003. Epub 2019 Dec 4. PMID: 31980368.
    • Type: Journal Articles Status: Published Year Published: 2020 Citation: Zhao M, Song K, Hao W, Wang L, Patil G, Li Q, Xu L, Hua F, Fu B, Schwamborn JC, Dorf ME, Li S. Non-proteolytic ubiquitination of OTULIN regulates NF-?B signaling pathway. J Mol Cell Biol. 2020 Apr 24;12(3):163-175. doi: 10.1093/jmcb/mjz081. PMID: 31504727; PMCID: PMC7181720.


    Progress 10/01/18 to 09/30/19

    Outputs
    Target Audience:This project covers agricultural and biomedical research funded by sources other than USDA that contributes to the total research capacity of the OSU College of Veterinary Medicine. We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects came from Federal, State, and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?These research opportunities provide training for graduate students, DVM trainees in clinical and pathology residency programs, and veterinary students during the summer and in a Research Elective course during the fall and spring semesters. How have the results been disseminated to communities of interest?Publications in scientific journals. Marketing pieces in newsletters, magazines and on website. What do you plan to do during the next reporting period to accomplish the goals?Continue to pursue the goals outlined. Additional short term seed grants have been awarded for the next period.

    Impacts
    What was accomplished under these goals? Short-term seed awards funded in 2018 resulted in 6 proposals for external funding, of which 4 are pending and one awarded. Three new short-term seed awards were funded in 2019. 1. Prevalence of anthelmintic resistance in Oklahoma beef cattle herds. There is increasing evidence that anthelmintic resistance is becoming a more prevalent issue in beef cattle production. Resistance has been identified in parasite populations in cattle from various parts of the United States. The only data specific to Oklahoma was reported through the 2007-2008 National Animal Health Monitoring Survey (NAHMS) Beef Cow-Calf study. Four producers from OK submitted samples for that study and two of the farms had evidence of anthelmintic resistance. The proposed study will determine the presence of anthelmintic resistance in 10-20 cooperating beef cattle herds from various regions in OK. 2. Influenza D pathogenesis and host immune responses in calves under single- and dual-infection. Disorders of the respiratory tract account for the highest percentage of deaths in calves due to nonpredators in all operation types ranging from 23% for beef to over 36% in mixed operations and lead to major economic losses to US producers. Most of the cases involve infectious agents, and identification of coinfections in bovine respiratory disease (BRD) cases is frequent. A new Influenza virus, Influenza D (IDV) was recently described in cattle and little is known about its pathogenesis, pathogenicity, clinical course and elicited immune responses in naïve calves. Bovine viral diarrhea virus (BVDV) infection in calves cause leads to transient immunosuppression, and the effects of BVDV on the immune system may lead to increased susceptibility of calves to other pathogens, including IDV. The overall goal of this project is to characterize the pathogenesis, clinical progression and immune responses of calves to IDV in a single (IDV only) and dual infection (BVDV+IDV) models. 3. Construction of a bRSV infectious clone and establishment of an experimental infection model of bRSV in neonatal calves. Bovine respiratory syncytial virus (bRSV) is a single-strand RNA virus from the Orthopneumovirus genus within the family Pneumoviridae (previously classified within the Paramyxoviridae). bRSV is a major player in the Bovine Respiratory Disease Complex (BRDC) in calves, resulting in significant economic losses to the beef and dairy industry. Calves under 1 year of age are most susceptible to bRSV, which acts as a primary pathogen causing pneumonia and predisposes calves to secondary viral and bacterial infections. Commercial vaccines are available for veterinary use, although hampered by variable field safety and efficacy. We propose to develop a bRSV cDNA clone for infectious virus generation and to establish a neonatal calf bRSV infection model, with translational applications. Equipment Multiple research laboratories in the College of Veterinary Medicine conduct investigations into specialized interactions between cells, including multiple cell tissues within an organ. These interactions include host-pathogen interaction, including cells of the immune system or other organs such as the lung. Other cell-based studies include stem cells and tissue regeneration, growth and development, changes associated with metabolic diseases such as diabetes and obesity, changes associated with aging, or changes associated with muscle activity in exercise. To facilitate these studies we have purchased a Cell Imaging Multi-Mode Microplate Reader, that will: 1) measure signals from cells in plates using several types of labels, including fluorescence at multiple wavelengths, luminescence, and other color-based stains and 2) allow digital images of those cells to be captured at multiple magnifications and overlaid to generate false-color and 3-D images of the cells for publication.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Allen, K.E., J.E. Thomas, M.L. Wohltjen, and M.V. Reichard, Transmission of Cytauxzoon felis to domestic cats by Amblyomma americanum nymphs. Parasites & vectors, 2019. 12(1): p. 28-28.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Ayalew, S., A.W. Confer, and M.B. Couger, Genome Sequence of a Bovine Isolate of Pasteurella multocida Strain 232. Microbiology resource announcements, 2019. 8(20): p. e00333-19.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Balasubramanian, P., D. Hall, and M. Subramanian, Sympathetic nervous system as a target for aging and obesity-related cardiovascular diseases. GeroScience, 2019. 41(1): p. 13-24.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Confer, A.W. and S. Ayalew, Mannheimia haemolytica in bovine respiratory disease: immunogens, potential immunogens, and vaccines. Animal health research reviews, 2018. 19(2): p. 79-99.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Davis, M.S., R.J. Geor, and K.K. Williamson, Effect of Endurance Conditioning on Insulin-mediated Glucose Clearance in Dogs. Medicine and science in sports and exercise, 2018. 50(12): p. 2494-2499.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Di Girolamo, N. and R. Meursinge Reynders, On "authors' knowledge" and contrast-enhanced ultrasonography in rabbits. Veterinary radiology & ultrasound: the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association, 2019. 60(4): p. 371-371.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Di Girolamo, N., Advances in Retrieval and Dissemination of Medical Information. The veterinary clinics of North America. Exotic animal practice, 2019. 22(3): p. 539-548.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: DiGeronimo, P.M. and J. Brand�o, Orthopedics in Reptiles and Amphibians. The veterinary clinics of North America. Exotic animal practice, 2019. 22(2): p. 285-300.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: d'Offay, J.M., R.W. Fulton, M. Fishbein, R. Eberle, and E.J. Dubovi, Isolation of a naturally occurring vaccine/wild-type recombinant bovine herpesvirus type 1 (BoHV-1) from an aborted bovine fetus. Vaccine, 2019. 37(32): p. 4518-4524.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: d'Offay, J.M., R.W. Fulton, R. Eberle, E.J. Dubovi, and C.C.L. Chase, Complete genome sequence of bovine herpesvirus type 1.1 (BoHV-1.1) Los Angeles (LA) strain and its genotypic relationship to BoHV-1.1 Cooper and more recently isolated wild-type field strains. Archives of virology, 2019. 164(11): p. 2843-2848.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Easton-Jones, C.A., J.E. Madigan, S. Barnum, L.K. Maxwell, S.D. Taylor, T. Arnesen, and N. Pusterla, Effect of valacyclovir on EHV-5 viral kinetics in horses with equine multinodular pulmonary fibrosis. Journal of veterinary internal medicine, 2018. 32(5): p. 1763-1767.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Greer, J.B., J.T. Magnuson, K. Hester, M. Giroux, C. Pope, T. Anderson, J. Liu, V. Dang, N.D. Denslow, and D. Schlenk, Effects of Chlorpyrifos on Cholinesterase and Serine Lipase Activities and Lipid Metabolism in Brains of Rainbow Trout (Oncorhynchus mykiss). Toxicological sciences : an official journal of the Society of Toxicology, 2019. 172(1): p. 146-154.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Jett, S., D. Laurence, R. Kunkel, A.R. Babu, K. Kramer, R. Baumwart, R. Towner, Y. Wu, and C.-H. Lee, An investigation of the anisotropic mechanical properties and anatomical structure of porcine atrioventricular heart valves. Journal of the mechanical behavior of biomedical materials, 2018. 87: p. 155-171.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Jett, S., D. Laurence, R. Kunkel, A.R. Babu, K. Kramer, R. Baumwart, R. Towner, Y. Wu, and C.-H. Lee, Biaxial mechanical data of porcine atrioventricular valve leaflets. Data in brief, 2018. 21: p. 358-363.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Laurence, D., C. Ross, S. Jett, C. Johns, A. Echols, R. Baumwart, R. Towner, J. Liao, P. Bajona, Y. Wu, and C.-H. Lee, Regional biaxial mechanical data of the mitral and tricuspid valve anterior leaflets. Data in brief, 2019. 24: p. 103961-103961.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Lee, C.-H., D.W. Laurence, C.J. Ross, K.E. Kramer, A.R. Babu, E.L. Johnson, M.-C. Hsu, A. Aggarwal, A. Mir, H.M. Burkhart, R.A. Towner, R. Baumwart, and Y. Wu, Mechanics of the Tricuspid Valve-From Clinical Diagnosis/Treatment, In-Vivo and In-Vitro Investigations, to Patient-Specific Biomechanical Modeling. Bioengineering (Basel, Switzerland), 2019. 6(2): p. 47.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: McGill, J.E., A.S. Hanzlicek, K.S. Kukanich, G.D. Norsworthy, and A.K. Cook, Pretreatment Histoplasma capsulatum urine enzymatic immunoassay concentrations do not correlate with outcome but may be influenced by renal function in cats with histoplasmosis. Journal of feline medicine and surgery, 2018. 20(12): p. 1177-1179.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Li, S., B. Fu, and C.D. Meshram, Innate Immune and Inflammatory Responses to Respiratory Viruses. Mediators of inflammation, 2019. 2019: p. 3146065-3146065.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Li, Y., X. Huang, F. Guo, T. Lei, S. Li, P. Monaghan-Nichols, Z. Jiang, H.-B. Xin, and M. Fu, TRIM65 E3 ligase targets VCAM-1 degradation to limit LPS-induced lung inflammation. Journal of molecular cell biology, 2019: p. mjz077.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Lyman, C.C., G.R. Holyoak, K. Meinkoth, X. Wieneke, K.A. Chillemi, and U. DeSilva, Canine endometrial and vaginal microbiomes reveal distinct and complex ecosystems. PloS one, 2019. 14(1): p. e0210157-e0210157.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Meshram, C.D. and A.G.P. Oomens, Identification of a human respiratory syncytial virus phosphoprotein domain required for virus-like-particle formation. Virology, 2019. 532: p. 48-54.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Miller, C., J. Powers, E. Musselman, R. Mackie, J. Elder, and S. VandeWoude, Immunopathologic Effects of Prednisolone and Cyclosporine A on Feline Immunodeficiency Virus Replication and Persistence. Viruses, 2019. 11(9): p. 805.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Mirniaharikandehei, S., J. VanOsdol, M. Heidari, G. Danala, S.N. Sethuraman, A. Ranjan, and B. Zheng, Developing a Quantitative Ultrasound Image Feature Analysis Scheme to Assess Tumor Treatment Efficacy Using a Mouse Model. Scientific reports, 2019. 9(1): p. 7293-7293.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Norton, E., N. Schultz, R. Geor, D. McFarlane, J. Mickelson, and M. McCue, Genome-Wide Association Analyses of Equine Metabolic Syndrome Phenotypes in Welsh Ponies and Morgan Horses. Genes, 2019. 10(11): p. E893.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Nagamori, Y., A. Ramachandran, C. Kuzma, L. Nafe, and E.M. Johnson, A zoonotic parasite, Linguatula serrata, infection in a dog imported from Ethiopia to the United States. Veterinary parasitology, regional studies and reports, 2019. 16: p. 100273-100273.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Nagamori, Y., M. Payton, L. Coburn, J.E. Thomas, and M. Reichard, Nymphal engorgement weight predicts sex of adult Amblyomma americanum, Amblyomma maculatum, Dermacentor andersoni, Dermacentor variabilis, and Rhipicephalus sanguineus ticks. Experimental & applied acarology, 2019. 77(3): p. 401-410.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Nagamori, Y., M.E. Payton, R. Duncan-Decocq, and E.M. Johnson, Fecal survey of parasites in free-roaming cats in northcentral Oklahoma, United States. Veterinary parasitology, regional studies and reports, 2018. 14: p. 50-53.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Piao, D., L.A. Sypniewski, D. Dugat, C. Bailey, D.J. Burba, and L. DeTaboada, Transcutaneous transmission of photobiomodulation light to the spinal canal of dog as measured from cadaver dogs using a multi-channel intra-spinal probe. Lasers in medical science, 2019. 34(8): p. 1645-1654.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Lim, A., B. Naidenov, H. Bates, K. Willyerd, T. Snider, M.B. Couger, C. Chen, and A. Ramachandran, Nanopore ultra-long read sequencing technology for antimicrobial resistance detection in Mannheimia haemolytica. Journal of microbiological methods, 2019. 159: p. 138-147.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Little, S.E., A.W. Barrett, M.J. Beall, D.D. Bowman, S. Dangoudoubiyam, D.A. Elsemore, J. Liotta, A. Lucio-Forster, D.J. McCrann, K.F. Snowden, L.A. Starkey, and S. Tasse, Coproantigen Detection Augments Diagnosis of Common Nematode Infections in Dogs. Topics in companion animal medicine, 2019. 35: p. 42-46.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Ludwig, H.C., A.S. Hanzlicek, K.S. KuKanich, and M.E. Payton, Candidate prognostic indicators in cats with histoplasmosis treated with antifungal therapy. Journal of feline medicine and surgery, 2018. 20(10): p. 985-996.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Schreeg, M.E., H.S. Marr, J.L. Tarigo, M.K. Sherrill, H.K. Outi, E.H. Scholl, D.M. Bird, A. Vigil, C. Hung, R. Nakajima, L. Liang, A. Trieu, D.L. Doolan, J.E. Thomas, M.G. Levy, M.V. Reichard, P.L. Felgner, L.A. Cohn, and A.J. Birkenheuer, Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis. Clinical proteomics, 2018. 15: p. 44-44.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Snyder, J.M., T.A. Snider, M.A. Ciol, J.E. Wilkinson, D.M. Imai, K.M. Casey, J.G. Vilches-Moure, C. Pettan-Brewer, S.P.S. Pillai, S.E. Carrasco, S. Salimi, and W. Ladiges, Validation of a geropathology grading system for aging mouse studies. GeroScience, 2019. 41(4): p. 455-465.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Taylor, J.D., J.N. Gilliam, G. Mourer, and C. Stansberry, Comparison of effects of four weaning methods on health and performance of beef calves. Animal: an international journal of animal bioscience, 2019: p. 1-10.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Schmidt, M.E., A.G.P. Oomens, and S.M. Varga, Vaccination with a Single-Cycle Respiratory Syncytial Virus Is Immunogenic and Protective in Mice. Journal of immunology (Baltimore, Md.: 1950), 2019. 202(11): p. 3234-3245.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Pulavendran, S., J.M. Rudd, P. Maram, P.G. Thomas, R. Akhilesh, J.R. Malayer, V.T. Chow, and N. Teluguakula, Combination therapy targeting platelet activation and virus replication protects mice against lethal influenza pneumonia. American journal of respiratory cell and molecular biology, 2019: p. 10.1165/rcmb.2018-0196OC.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Rudd, J.M., S. Pulavendran, H.K. Ashar, J.W. Ritchey, T.A. Snider, J.R. Malayer, M. Marie, V.T.K. Chow, and T. Narasaraju, Neutrophils Induce a Novel Chemokine Receptors Repertoire During Influenza Pneumonia. Frontiers in cellular and infection microbiology, 2019. 9: p. 108-108.
    • Type: Journal Articles Status: Published Year Published: 2019 Citation: Saleh, M.N., J.R. Heptinstall, E.M. Johnson, L.R. Ballweber, D.S. Lindsay, S. Werre, J.F. Herbein, and A.M. Zajac, Comparison of diagnostic techniques for detection of Giardia duodenalis in dogs and cats. Journal of veterinary internal medicine, 2019. 33(3): p. 1272-1277.


    Progress 10/01/17 to 09/30/18

    Outputs
    Target Audience:This project covers agricultural and biomedical research funded by sources other than USDA that contributes to the total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects came from Federal, State, and private funding sources, including biological and pharmaceutical corporations. Publications listed are representative of the studies funded. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?These research opportunities provide training for graduate students, DVM trainees in clinical and pathology residency programs, and veterinary students during the summer and in a Research Elective course during the fall and spring semesters. How have the results been disseminated to communities of interest?Publications in scientific journals. Marketing pieces in newsletters, magazines and on website. What do you plan to do during the next reporting period to accomplish the goals?Continue to pursue the goals outlined. Additional short-term seed grants have been awarded for the next period.

    Impacts
    What was accomplished under these goals? Five new short-term seed awards were funded in 2018. 1. A pilot study of nasopharyngeal microbiome of bovine respiratory disease complex. Nasal and deep nasopharyngeal swabs were collected from calves. The swabs were placed in ZR BashingBeadTM Lysis Tubes containing DNA/RNA ShieldTM and immediately transported to the laboratory for processing. RNA and DNA were extracted and both sets of nucleic acid preps were quantified and submitted for sequencing. 16S rRNA sequences were analyzed using MOTHUR. RNA sequences were analyzed by using BOWTIE in MacVector. The metagenomics data was processed with Megahit. All datasets were quality screened to filter all the sequences with lengths less than 50 base pairs, sequences with ambiguous nucleotides, and sequences with hompolymer stretches more than 8 bps. Sequences were classified using classify.seqs commands package in MOTHUR, using Silva alignment and Greengenes classification scheme, the phylogenetic affiliations on the phylum, class and general levels of the 16S rRNA sequences were identified using a cutoff of 80% confidence threshold. All analyses were conducted on the HPC Cowboy supercomputer, a 252 compute nodes with dual six-core CPUs and 32 GB RAMs server, 2 fat nodes with 256 GB RAM, GPU cards and 120 TB very fast disk storage at the OSU High-Performance Computing Center at Oklahoma State University. Organisms belonging to 42 phyla were identified in the swabs collected for this study. Overall nasal swabs have yielded substantially more phyla than deep nasopharyngeal swabs. This was exactly as expected. A full-length manuscript is currently in preparation. 2. The role of hyperthermia in exertional rhabdomyolysis. This study is complete. The resulting data supported the hypothesis that hyperthermia results in progressive uncoupling of oxidative phosphorylation of equine skeletal muscle, resulting in the partial failure of oxygen consumption to produce ATP to power skeletal muscle contraction. In exercising horses, this phenomenon will result in overheating and eventual fatigue, with possible heat-related injury. These data have been used to support a multi-year extramural research proposal to the Grayson Jockey Club Research Foundation, with goals to include the investigation of skeletal muscle acidosis on oxidative phosphorylation as well as the potential for exercise conditioning to partially ameliorate the mitochondrial dysfunction caused by these physiochemical extremes. The results from this study were accepted through a peer-reviewed abstract submission for presentation at the 2018 International Conference on Equine Exercise Physiology. A full-length manuscript is currently in preparation. 3. Characterization of short- and long-term morbidity and mortality of goat kids born to does with pregnancy toxemia. We have collected all the preg tox kid data needed for the study. So far the kids have shown marked acidemia and hypoxemia compared to control kids, with an increased perinatal mortality. Ongoing. 4. Equine atrial fibrillation: Novel cause and treatment. In horses, as in humans, atrial fibrillation (AF) is the most common sustained cardiac arrhythmia, negatively affecting athletic performance and the cardiac health of leisure and sport horses alike. So far, the only associated risk factor for AF is a high body weight. However, to our knowledge, epidemiological studies have not yet investigated whether equine metabolic syndrome is a risk factor for the development of AF. Furthermore, given the frequency of AF in horses worldwide and the severity of outcomes, treatment options for this disorder are needed. Ongoing. 5. Effect of caffeic acid phenethyl ester on lipopolysaccharide-induced cytokine production in equine pulmonary macrophages. Inflammatory airway disease, recurrent airway obstruction, and summer pasture-associated recurrent airway obstruction are non-infectious inflammatory respiratory diseases of the horse now collectively known as equine asthma. Airway hyperresponsiveness increased airway mucus production, and varying degrees of bronchoconstriction are hallmarks of equine asthma. Our long-term goal is to develop alternatives to corticosteroids for the treatment of equine asthma. Nuclear factor kappa B (NF-KB) is a transcription factor activated in asthma and turns on multiple pro-inflammatory genes, leading to amplification of the inflammatory response, and presents an attractive target for potential therapeutics. Caffeic acid phenethyl ester (CAPE), an extract from honeybee hives, is a specific inhibitor of NF-KB. In an experimental model of asthma, mice treated with CAPE intraperitoneally showed reduced airway hypersensitivity, inflammatory cell infiltration, mucus hypersecretion, collagen deposition, fibrosis, and levels of TNF-a and TGF-B1. In macrophages harvested from BAL fluid collected from horses, we have shown that CAPE reduced TNF-a production in a dose-dependent manner. The current preliminary information was used to acquire funding from Boehringer Ingelheim for an in-vivo project using nebulized CAPE in horses.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Ashar HK, Mueller NC, Rudd JM, Snider TA, Achanta M, Prasanthi M, Pulavendran S, Thomas PG, Ramachandran A, Malayer JR, Ritchey JW, Rajasekhar R, Chow VTK, Esmon CT, Teluguakula N. The Role of Extracellular Histones in Influenza Virus Pathogenesis. Am J Pathol. 2018 Jan;188(1):135-148. doi: 10.1016/j.ajpath.2017.09.014. Epub 2017 Oct 26. PubMed PMID: 29107075; PubMed Central PMCID: PMC5745522.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Eberle R, Jones-Engel L. Questioning the Extreme Neurovirulence of Monkey B Virus (Macacine alphaherpesvirus 1). Adv Virol. 2018 Feb 13;2018:5248420. doi: 10.1155/2018/5248420. eCollection 2018. Review. PubMed PMID: 29666644; PubMed Central PMCID: PMC5831965.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Fulton RW, Confer AW, Sorensen NJ, Ridpath JF, Burge LJ. Bovine viral diarrhea virus 1b fetal infection with extensive hemorrhage. J Vet Diagn Invest. 2017 Nov;29(6):880-884. doi: 10.1177/1040638717726825. Epub 2017 Aug 14. PubMed PMID: 28803536.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Fulton RW, Neill JD, Saliki JT, Landis C, Burge LJ, Payton ME. Genomic and antigenic characterization of bovine parainfluenza-3 viruses in the United States including modified live virus vaccine (MLV) strains and field strains from cattle. Virus Res. 2017 May 2;235:77-81. doi: 10.1016/j.virusres.2017.04.009. Epub 2017 Apr 14. PubMed PMID: 28416404.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Halleran JL, Baumwart RD, Dugat DR, Streeter RN, Phillips H. Surgical ligation of patent ductus arteriosus in a dorper sheep. Vet Surg. 2018 Oct 26. doi: 10.1111/vsu.12969. [Epub ahead of print] PubMed PMID: 30365170.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Banse HE, Schultz N, McCue M, Geor R, McFarlane D. Comparison of two methods for measurement of equine adrenocorticotropin. J Vet Diagn Invest. 2018 Mar;30(2):233-237. doi: 10.1177/1040638717752216. Epub 2017 Dec 28. PubMed PMID: 29284383.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Jamieson CA, Hanzlicek AS, Payton ME, Holbrook TC. Normal reference intervals and the effects of sample handling on dynamic viscoelastic coagulometry (Sonoclot) in healthy adult horses. J Vet Emerg Crit Care (San Antonio). 2018 Jan;28(1):39-44. doi: 10.1111/vec.12687. Epub 2017 Dec 15. PubMed PMID: 29244257.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Bhowmick R, Derakhshan T, Liang Y, Ritchey J, Liu L, Gappa-Fahlenkamp H. A Three-Dimensional Human Tissue-Engineered Lung Model to Study Influenza A Infection. Tissue Eng Part A. 2018 Oct;24(19-20):1468-1480. doi: 10.1089/ten.TEA.2017.0449. Epub 2018 Jun 29. PubMed PMID: 29732955; PubMed Central PMCID: PMC6198767.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Easton-Jones CA, Madigan JE, Barnum S, Maxwell LK, Taylor SD, Arnesen T, Pusterla N. Effect of valacyclovir on EHV-5 viral kinetics in horses with equine multinodular pulmonary fibrosis. J Vet Intern Med. 2018 Sep;32(5):1763-1767. doi: 10.1111/jvim.15230. Epub 2018 Sep 17. PubMed PMID: 30221792; PubMed Central PMCID: PMC6189341.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Huang C, Xiao X, Yang Y, Mishra A, Liang Y, Zeng X, Yang X, Xu D, Blackburn MR, Henke CA, Liu L. MicroRNA-101 attenuates pulmonary fibrosis by inhibiting fibroblast proliferation and activation. J Biol Chem. 2017 Oct 6;292(40):16420-16439. doi: 10.1074/jbc.M117.805747. Epub 2017 Jul 18. PubMed PMID: 28726637; PubMed Central PMCID: PMC5633105.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Jett S, Laurence D, Kunkel R, Babu AR, Kramer K, Baumwart R, Towner R, Wu Y, Lee CH. Biaxial mechanical data of porcine atrioventricular valve leaflets. Data Brief. 2018 Oct 3;21:358-363. doi: 10.1016/j.dib.2018.09.073. eCollection 2018 Dec. PubMed PMID: 30364794; PubMed Central PMCID: PMC6197746.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Johnson WL, Ramachandran A, Torres NJ, Nicholson AC, Whitney AM, Bell M, Villarma A, Humrighouse BW, Sheth M, Dowd SE, McQuiston JR, Gustafson JE. The draft genomes of Elizabethkingia anophelis of equine origin are genetically similar to three isolates from human clinical specimens. PLoS One. 2018 Jul 19;13(7):e0200731. doi: 10.1371/journal.pone.0200731. eCollection 2018. PubMed PMID: 30024943; PubMed Central PMCID: PMC6053191.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Maria Z, Campolo AR, Scherlag BJ, Ritchey JW, Lacombe VA. Dysregulation of insulin-sensitive glucose transporters during insulin resistance-induced atrial fibrillation. Biochim Biophys Acta Mol Basis Dis. 2018 Apr;1864(4 Pt A):987-996. doi: 10.1016/j.bbadis.2017.12.038. Epub 2017 Dec 29. PubMed PMID: 29291943.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Maria Z, Lacombe VA. Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay. Methods Mol Biol. 2018;1713:229-240. doi: 10.1007/978-1-4939-7507-5_17. PubMed PMID: 29218529.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Nigatu AS, Ashar H, Sethuraman SN, Wardlow R, Maples D, Malayer J, Ranjan A. Elastin-like polypeptide incorporated thermally sensitive liposome improve antibiotic therapy against musculoskeletal bacterial pathogens. Int J Hyperthermia. 2018 Mar;34(2):201-208. doi: 10.1080/02656736.2017.1420249. PubMed PMID: 29278945.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Schoonover MJ, Moser DK, Young JM, Payton ME, Holbrook TC. Effects of tourniquet number and exsanguination on amikacin concentrations in the radiocarpal and distal interphalangeal joints after low volume intravenous regional limb perfusion in horses. Vet Surg. 2017 Jul;46(5):675-682. doi: 10.1111/vsu.12662. Epub 2017 Apr 29. PubMed PMID: 28460426.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Senavirathna LK, Huang C, Yang X, Munteanu MC, Sathiaseelan R, Xu D, Henke CA, Liu L. Hypoxia induces pulmonary fibroblast proliferation through NFAT signaling. Sci Rep. 2018 Feb 9;8(1):2709. doi: 10.1038/s41598-018-21073-x. PubMed PMID: 29426911; PubMed Central PMCID: PMC5807313.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Thomas M, Fenske GJ, Antony L, Ghimire S, Welsh R, Ramachandran A, Scaria J. Whole genome sequencing-based detection of antimicrobial resistance and virulence in non-typhoidal Salmonella enterica isolated from wildlife. Gut Pathog. 2017 Nov 21;9:66. doi: 10.1186/s13099-017-0213-x. eCollection 2017. PubMed PMID: 29201148; PubMed Central PMCID: PMC5697165.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Patil G, Zhao M, Song K, Hao W, Bouchereau D, Wang L, Li S. TRIM41-Mediated Ubiquitination of Nucleoprotein Limits Influenza A Virus Infection. J Virol. 2018 Jul 31;92(16). pii: e00905-18. doi: 10.1128/JVI.00905-18. Print 2018 Aug 15. PubMed PMID: 29899090; PubMed Central PMCID: PMC6069172.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Sawant L, Kook I, Vogel JL, Kristie TM, Jones C. The Cellular Coactivator HCF-1 Is Required for Glucocorticoid Receptor-Mediated Transcription of Bovine Herpesvirus 1 Immediate Early Genes. J Virol. 2018 Aug 16;92(17). pii: e00987-18. doi: 10.1128/JVI.00987-18. Print 2018 Sep 1. PubMed PMID: 29899098; PubMed Central PMCID: PMC6096806.
    • Type: Journal Articles Status: Published Year Published: 2018 Citation: Workman A, Zhu L, Keel BN, Smith TPL, Jones C. The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency. J Virol. 2018 Mar 14;92(7). pii: e01937-17. doi: 10.1128/JVI.01937-17. Print 2018 Apr 1. PubMed PMID: 29321317; PubMed Central PMCID: PMC5972910.


    Progress 10/01/16 to 09/30/17

    Outputs
    Target Audience:This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non USDA projects came from Federal, State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?These research opportunities provide training for graduate students, DVM trainees in clinical and pathology residency programs, and veterinary students during the summer and in a Research Elective course during the fall and spring semesters. How have the results been disseminated to communities of interest?Publications in scientific journals. What do you plan to do during the next reporting period to accomplish the goals?Continue to pursue the goals outlined. Additional short term seed grants have been awarded for the next period.

    Impacts
    What was accomplished under these goals? The interdisciplinary research activity in the CVHS centered on focus areas in infectious disease, parasitology, health and performance, and animal models of human disease, provides an underlying infrastructure for basic research into veterinary science and medicine. This infrastructure adds value to our service and teaching missions, and provides a critical resource for Oklahoma in animal and public health. Five new short-term seed awards were funded in the fall of 2017. 1. A pilot study of nasopharyngeal microbiome of bovine respiratory disease complex 2. The role of hyperthermia in exertional rhabdomyolysis 3. Characterization of short- and long-term morbidity and mortality of goat kids born to does with pregnancy toxemia 4. Equine atrial fibrilllation: Novel cause and treatment 5. Effect of caffeic acid phenethyl ester on lipopolysaccharide-induced cytokine production in equine pulmonary macrophages Two new short-term seed awards were funded in the fall of 2016. Proteoglycans and Insulin Resistance: Novel Mechanisms - The focus is on how proteoglycans regulate glucose metabolism in insulin sensitive tissue with the intent of identifying molecular therapeutic targets for insulin resistance. The overall broad long-term objective is to better understand the impact of reduced proteoglycan glycosaminoglycans on glucose metabolism in insulin-sensitive tissue (i.e., cardiac and skeletal muscle and adipose tissue). Due to the lack of proteoglycans in our lipodystrophic mouse model we have found novel alterations in the levels of several insulin sensitive glucose transporters. The greatest changes we have found are increases in adipose tissue Glut 4 and Glut 8. Surprisingly there were alterations in Glut 4 in brown adipose tissue. The liver displayed alterations in Glut 8 as well. At present we are trying to connect these changes in light of insulin responsiveness and glucose levels since these are responsive glucose transporters. We believe these are novel findings in lipodystrophy. Targeted Thermosensitive Nanoparticle Therapy for Treatment of Equine Endocrinopathic Laminitis - The intent of this study is to develop novel therapeutics for the treatment of laminitis using thermosensitive nanoparticles for directed drug delivery to the equine foot. In addition, this project seeks to critically assess the central hypothesis that hyperinsulinemia initiates laminitis by activation of the IGF-1 receptor (IGF-1R). Recent research has robustly shown that hyperinsulinemia can induce equine endocrinopathic laminitis. Due to the absence of insulin receptors in the affected region of the foot (secondary lamellae), it is hypothesized that hyperinsulinemia triggers laminitis through activation of the IGF-1 receptor. To test this hypothesis, we administered a continuous infusion of IGF-1 into a single front foot through an arterial catheter placed in the medial metacarpal artery, with the contralateral foot serving as an intra-animal control. In the initial horse IGF-1 was administered at 1 mg over 48 hours and mild signs of discomfort with histological evidence of laminitis were induced in the treated foot. Unfortunately, there was also histological evidence of historical laminitis in this animal. Due to the mild nature of the observed changes, the dose was increased 5-fold for the following 3 horses. At this dose, the IGF-1 treatment did not induce clinical or histological changes. Therefore an additional horse was treated at the low dose, and this horse showed moderate to marked clinical and histological signs in the treated limb only. Western blot analysis of the hoof tissue is ongoing to determine receptor activation in treated limbs and control limbs. Analysis of IGF-1 concentration in samples collected from systemic and distal limb blood is also ongoing. Due to this unanticipated dose response with induction of disease occurring only with the low dose in 2 horses, it is necessary to do additional horses to increase confidence in the results. Further optimization of the model is also necessary as several horses had failure of the arterial catheter prior to study completion, resulting in their exclusion from the study. However, this preliminary data does suggest further investigation of this model is warranted as receptor antagonists are available for the IGF-1R and may provide a targeted treatment for endocrinopathic laminitis.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Ashar HK, Mueller NC, Rudd JM, Snider TA, Achanta M, Prasanthi M, Pulavendran S, Thomas PG, Akhilesh R, Malayer JR, Ritchey JW, Rajasekhar R, Chow VT, Esmon CT, Teluguakula N. The role of extracellular histones in influenza virus pathogenesis. Am J Pathol. 2017 Oct 26. pii: S0002-9440(17)30412-1. doi: 10.1016/j.ajpath.2017.09.014. [Epub ahead of print] PubMed PMID: 29107075.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Ayalew S, Confer AW, Hansen RD, Couger MB. Genome Sequence of a Spontaneous Nonhemolytic Mutant of Mannheimia haemolytica 16041065 GH. Genome Announc. 2017 Apr 6;5(14). pii: e01720-16. doi: 10.1128/genomeA.01720-16. PubMed PMID: 28385858; PubMed Central PMCID: PMC5383906.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Ayalew S, Confer AW, Hansen RD, Couger MB. Genome Sequence of Mannheimia haemolytica Serotype 1 Strain 16041065 BH. Genome Announc. 2017 Apr 6;5(14). pii: e01721-16. doi: 10.1128/genomeA.01721-16. PubMed PMID: 28385859; PubMed Central PMCID: PMC5383907.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Ayalew S, Confer AW, Hartson SD, Canaan PJ, Payton M, Couger B. Proteomic and bioinformatic analyses of putative Mannheimia haemolytica secretome by liquid chromatography and tandem mass spectrometry. Vet Microbiol. 2017 May;203:73-80. doi: 10.1016/j.vetmic.2017.02.011. Epub 2017 Feb 27. PubMed PMID: 28619171.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Baumwart RD, Hanzlicek AS, Lyon SD, Lee PM. Plasma N-terminal pro-brain natriuretic peptide concentrations before and after pericardiocentesis in dogs with cardiac tamponade secondary to spontaneous pericardial effusion. J Vet Cardiol. 2017 Oct;19(5):416-420. doi: 10.1016/j.jvc.2017.08.007. Epub 2017 Oct 4. PubMed PMID: 28988688.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Bharadwaj M, Pope C, Davis M, Katz S, Cook C, Maxwell L. Subacute pyridostigmine exposure increases heart rate recovery and cardiac parasympathetic tone in rats. Clin Exp Pharmacol Physiol. 2017 Aug;44(8):872-879. doi: 10.1111/1440-1681.12773. PubMed PMID: 28440910; PubMed Central PMCID: PMC5513757.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Boyoglu-Barnum S, Todd SO, Meng J, Barnum TR, Chirkova T, Haynes LM, Jadhao SJ, Tripp RA, Oomens AG, Moore ML, Anderson LJ. Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective. J Virol. 2017 Apr 28;91(10). pii: e02059-16. doi: 10.1128/JVI.02059-16. Print 2017 May 15. PubMed PMID: 28275196; PubMed Central PMCID: PMC5411601.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Chase CCL, Fulton RW, O'Toole D, Gillette B, Daly RF, Perry G, Clement T. Bovine herpesvirus 1 modified live virus vaccines for cattle reproduction: Balancing protection with undesired effects. Vet Microbiol. 2017 Jul;206:69-77. doi: 10.1016/j.vetmic.2017.03.016. Epub 2017 Mar 23. PubMed PMID: 28377131.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Eberle R, Black DH. Sequence of the ateline alphaherpesvirus 1 (HVA1) genome. Arch Virol. 2017 May;162(5):1423-1425. doi: 10.1007/s00705-017-3249-9. Epub 2017 Feb 3. PubMed PMID: 28160144.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Eberle R, Maxwell LK, Nicholson S, Black D, Jones-Engel L. Genome sequence variation among isolates of monkey B virus (Macacine alphaherpesvirus 1) from captive macaques. Virology. 2017 Aug;508:26-35. doi: 10.1016/j.virol.2017.05.001. Epub 2017 May 7. PubMed PMID: 28494342; PubMed Central PMCID: PMC5535784.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Fulton RW, Confer AW, Sorensen NJ, Ridpath JF, Burge LJ. Bovine viral diarrhea virus 1b fetal infection with extensive hemorrhage. J Vet Diagn Invest. 2017 Nov;29(6):880-884. doi: 10.1177/1040638717726825. Epub 2017 Aug 14. PubMed PMID: 28803536.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Fulton RW, Neill JD, Saliki JT, Landis C, Burge LJ, Payton ME. Genomic and antigenic characterization of bovine parainfluenza-3 viruses in the United States including modified live virus vaccine (MLV) strains and field strains from cattle. Virus Res. 2017 May 2;235:77-81. doi: 10.1016/j.virusres.2017.04.009. Epub 2017 Apr 14. PubMed PMID: 28416404.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Herrin BH, Peregrine AS, Goring J, Beall MJ, Little SE. Canine infection with Borrelia burgdorferi, Dirofilaria immitis, Anaplasma spp. and Ehrlichia spp. in Canada, 2013-2014. Parasit Vectors. 2017 May 19;10(1):244. doi: 10.1186/s13071-017-2184-7. PubMed PMID: 28526093; PubMed Central PMCID: PMC5437676.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Piao D, Ramadan M, Park A, Bartels KE, Patel SG. Freehand diffuse optical spectroscopy imaging for intraoperative identification of major venous and arterial vessels underlying peritoneal fat: an in vivo demonstration in a pig model. J Biomed Opt. 2017 Oct;22(10):1-4. doi: 10.1117/1.JBO.22.10.100503. PubMed PMID: 29086545.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Miller B, Hamilton K, Boushel R, Williamson K, Laner V, Gnaiger E, Davis M. Mitochondrial respiration in highly aerobic canines in the non-raced state and after a 1600-km sled dog race. PLoS One. 2017 Apr 26;12(4):e0174874. doi: 10.1371/journal.pone.0174874. eCollection 2017. PubMed PMID: 28445477; PubMed Central PMCID: PMC5405936.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Mitcham JR, Barrett AW, Gruntmeir JM, Holland T, Martin JE, Johnson EM, Little SE, Noden BH. Active surveillance to update county scale distribution of four tick species of medical and veterinary importance in Oklahoma. J Vector Ecol. 2017 Jun;42(1):60-73. doi: 10.1111/jvec.12240. PubMed PMID: 28504425.
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Narasaraju T, Harshini A. Neutrophils as Possible Therapeutic Targets in Severe Influenza Pneumonia. J Infect Pulm Dis. 2016;2(2). doi: 10.16966/2470-3176.115. Epub 2016 May 31. PubMed PMID: 27868106; PubMed Central PMCID: PMC5113818.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Pollard DA, Reichard MV, Cohn LA, James AM, Holman PJ. Genetic variability of cloned Cytauxzoon felis ribosomal RNA ITS1 and ITS2 genomic regions from domestic cats with varied clinical outcomes from five states. Vet Parasitol. 2017 Sep 15;244:136-143. doi: 10.1016/j.vetpar.2017.08.002. Epub 2017 Aug 3. PubMed PMID: 28917305.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Pucket JD, Allbaugh RA, Higginbotham ML, Rankin AJ, Teixeira L. Metastatic intraocular hemangiopericytoma in a dog. Open Vet J. 2017;7(2):132-138. doi: 10.4314/ovj.v7i2.9. Epub 2017 May 22. PubMed PMID: 28652979; PubMed Central PMCID: PMC5471746.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Reichard MV, Thomas JE, Chavez-Suarez M, Cullin CO, White GL, Wydysh EC, Wolf RF. Pilot Study to Assess the Efficacy of Ivermectin and Fenbendazole for Treating Captive-Born Olive Baboons (Papio anubis) Coinfected with Strongyloides f�lleborni and Trichuris trichiura. J Am Assoc Lab Anim Sci. 2017 Jan 1;56(1):52-56. PubMed PMID: 28905715; PubMed Central PMCID: PMC5250495.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Sahoo K, Karumuri S, Hikkaduwa Koralege RS, Flynn NH, Hartson S, Liu J, Ramsey JD, Kalkan AK, Pope C, Ranjan A. Molecular and Biocompatibility Characterization of Red Blood Cell Membrane Targeted and Cell-Penetrating-Peptide-Modified Polymeric Nanoparticles. Mol Pharm. 2017 Jul 3;14(7):2224-2235. doi: 10.1021/acs.molpharmaceut.7b00053. Epub 2017 Jun 21. PubMed PMID: 28505457.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Saleh MN, Thomas JE, Heptinstall JR, Herbein JF, Wolf RF, Reichard MV, Zajac AM. Immunologic detection of Giardia duodenalis in a specific pathogen-free captive olive baboon (Papio cynocephalus anubis) colony. J Vet Diagn Invest. 2017 Nov;29(6):916-919. doi: 10.1177/1040638717721580. Epub 2017 Jul 28. PubMed PMID: 28754076.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Skinner D, Mitcham JR, Starkey LA, Noden BH, Fairbanks WS, Little SE. Prevalence of Babesia spp., Ehrlichia spp., and tick infestations in Oklahoma black bears (Ursus Americanus). J Wildl Dis. 2017 Oct;53(4):781-787. doi: 10.7589/2017-02-029. Epub 2017 Jun 28. PubMed PMID: 28657860.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Thomas JE, Ohmes CM, Payton ME, Hostetler JA, Reichard MV. Minimum transmission time of Cytauxzoon felis by Amblyomma americanum to domestic cats in relation to duration of infestation, and investigation of ingestion of infected ticks as a potential route of transmission. J Feline Med Surg. 2017 Feb 1:1098612X17691172. doi: 10.1177/1098612X17691172. [Epub ahead of print] PubMed PMID: 28151047.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: VanOsdol J, Ektate K, Ramasamy S, Maples D, Collins W, Malayer J, Ranjan A. Sequential HIFU heating and nanobubble encapsulation provide efficient drug penetration from stealth and temperature sensitive liposomes in colon cancer. J Control Release. 2017 Feb 10;247:55-63. doi: 10.1016/j.jconrel.2016.12.033. Epub 2016 Dec 30. PubMed PMID: 28042085; PubMed Central PMCID: PMC5293599.
    • Type: Journal Articles Status: Published Year Published: 2017 Citation: Wang X, Wu W, Zhang W, Leland Booth J, Duggan ES, Tian L, More S, Zhao YD, Sawh RN, Liu L, Zou MH, Metcalf JP. RIG-I overexpression decreases mortality of cigarette smoke exposed mice during influenza A virus infection. Respir Res. 2017 Sep 2;18(1):166. doi: 10.1186/s12931-017-0649-z. PubMed PMID: 28865477; PubMed Central PMCID: PMC5581920.


    Progress 10/01/15 to 09/30/16

    Outputs
    Target Audience:This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non USDA projects came from Federal, State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?These research opportunities provide training for graduate students, DVM trainees in clinical and pathology residency programs, and veterinary students during the summer and in a Research Elective course during the fall and spring semesters. How have the results been disseminated to communities of interest?Publications in scientific journals. What do you plan to do during the next reporting period to accomplish the goals?Continue to pursue the goals outlined. Additional short term seed grants will be awarded in the next period.

    Impacts
    What was accomplished under these goals? The interdisciplinary research activity in the CVHS centered on focus areas in infectious disease, parasitology, health and performance, and animal models of human disease, provides an underlying infrastructure for basic research into veterinary science and medicine. This infrastructure adds value to our service and teaching missions, and provides a critical resource for Oklahoma in animal and public health. Two new short-term seed awards were funded in the fall of 2016. Proteoglycans and Insulin Resistance: Novel Mechanisms - The focus is on how proteoglycans regulate glucose metabolism in insulin sensitive tissue with the intent of identifying molecular therapeutic targets for insulin resistance. The overall broad long-term objective is to better understand the impact of reduced proteoglycan glycosaminoglycans on glucose metabolism in insulin-sensitive tissue (i.e., cardiac and skeletal muscle and adipose tissue). Targeted Thermosensitive Nanoparticle Therapy for Treatment of Equine Endocrinopathic Laminitis - The intent of this study is to develop novel therapeutics for the treatment of laminitis using thermosensitive nanoparticles for directed drug delivery to the equine foot. In addition, this project seeks to critically assess the central hypothesis that hyperinsulinemia initiates laminitis by activation of the IGF-1 receptor (IGF-1R).

    Publications

    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Campolo A, de Laat MA, Keith L, Gruntmeir KJ, Lacombe VA. Prolonged hyperinsulinemia affects metabolic signal transduction markers in a tissue specific manner. Domest Anim Endocrinol. 2016 Apr;55:41-5. doi: 10.1016/j.domaniend.2015.11.001. PubMed PMID: 26773366.
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Confer AW, Snider TA, Taylor JD, Montelongo M, Sorensen NJ. Clinical disease and lung lesions in calves experimentally inoculated with Histophilus somni five days after metaphylactic administration of tildipirosin or tulathromycin. Am J Vet Res. 2016 Apr;77(4):358-66. doi: 10.2460/ajvr.77.4.358. PubMed PMID: 27027834.
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: de la Fuente J, Estrada-Pena A, Cabezas-Cruz A, Kocan KM. Anaplasma phagocytophilum Uses Common Strategies for Infection of Ticks and Vertebrate Hosts. Trends Microbiol. 2016 Mar;24(3):173-80. doi: 10.1016/j.tim.2015.12.001. Review. PubMed PMID: 26718986.
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: d'Offay JM, Eberle R, Fulton RW, Kirkland PD. Complete genomic sequence and comparative analysis of four genital and respiratory isolates of bovine herpesvirus subtype 1.2b (BoHV-1.2b), including the prototype virus strain K22. Arch Virol. 2016 Nov;161(11):3269-74. doi: 10.1007/s00705-016-3026-1. PubMed PMID: 27568014.
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Fulton RW, d'Offay JM, Dubovi EJ, Eberle R. Bovine herpesvirus-1: Genetic diversity of field strains from cattle with respiratory disease, genital, fetal disease and systemic neonatal disease and their relationship to vaccine strains. Virus Res. 2016 Sep 2;223:115-21. doi: 10.1016/j.virusres.2016.06.017. PubMed PMID: 27374060.
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Fulton RW, d'Offay JM, Landis C, Miles DG, Smith RA, Saliki JT, Ridpath JF, Confer AW, Neill JD, Eberle R, Clement TJ, Chase CC, Burge LJ, Payton ME. Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease. Vaccine. 2016 Jun 24;34(30):3478-92. doi: 10.1016/j.vaccine.2016.04.020. PubMed PMID: 27108192.
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Gulia-Nuss M, Nuss AB, Meyer JM, Sonenshine DE, Roe RM, Waterhouse RM, Sattelle DB, de la Fuente J, Ribeiro JM, Megy K, Thimmapuram J, Miller JR, Walenz BP, Koren S, Hostetler JB, Thiagarajan M, Joardar VS, Hannick LI, Bidwell S, Hammond MP, Young S, Zeng Q, Abrudan JL, Almeida FC, Ayllón N, Bhide K, Bissinger BW, Bonzon-Kulichenko E, Buckingham SD, Caffrey DR, Caimano MJ, Croset V, Driscoll T, Gilbert D, Gillespie JJ, Giraldo-Calderon GI, Grabowski JM, Jiang D, Khalil SM, Kim D, Kocan KM, Koci J, Kuhn RJ, Kurtti TJ, Lees K, Lang EG, Kennedy RC, Kwon H, Perera R, Qi Y, Radolf JD, Sakamoto JM, Sanchez-Gracia A, Severo MS, Silverman N, Simo L, Tojo M, Tornador C, Van Zee JP, Vazquez J, Vieira FG, Villar M, Wespiser AR, Yang Y, Zhu J, Arensburger P, Pietrantonio PV, Barker SC, Shao R, Zdobnov EM, Hauser F, Grimmelikhuijzen CJ, Park Y, Rozas J, Benton R, Pedra JH, Nelson DR, Unger MF, Tubio JM, Tu Z, Robertson HM, Shumway M, Sutton G, Wortman JR, Lawson D, Wikel SK, Nene VM, Fraser CM, Collins FH, Birren B, Nelson KE, Caler E, Hill CA. Genomic insights into the Ixodes scapularis tick vector of Lyme disease. Nat Commun. 2016 Feb 9;7:10507. doi: 10.1038/ncomms10507. PubMed PMID: 26856261; PubMed Central PMCID: PMC4748124.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Larios Mora A, Detalle L, Van Geelen A, Davis MS, Stohr T, Gallup JM, Ackermann MR. Kinetics of Respiratory Syncytial Virus (RSV) Memphis Strain 37 (M37) Infection in the Respiratory Tract of Newborn Lambs as an RSV Infection Model for Human Infants. PLoS One. 2015 Dec 7;10(12):e0143580. doi: 10.1371/journal.pone.0143580. PubMed PMID: 26641081; PubMed Central PMCID: PMC4671688.
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Wilson BK, Vazquez-Anon M, Step DL, Moyer KD, Haviland CL, Maxwell CL, O'Neill CF, Gifford CA, Krehbiel CR, Richards CJ. Effect of copper, manganese, and zinc supplementation on the performance, clinical signs, and mineral status of calves following exposure to bovine viral diarrhea virus type 1b and subsequent infection. J Anim Sci. 2016 Mar;94(3):1123-40. doi: 10.2527/jas.2015-9503. PubMed PMID: 27065274.
    • Type: Journal Articles Status: Published Year Published: 2016 Citation: Wilson BK, Holland BP, Step DL, Jacob ME, VanOverbeke DL, Richards CJ, Nagaraja TG, Krehbiel CR. Feeding wet distillers grains plus solubles with and without a direct-fed microbial to determine performance, carcass characteristics, and fecal shedding of O157:H7 in feedlot heifers. J Anim Sci. 2016 Jan;94(1):297-305. doi: 10.2527/jas.2015-9601. PubMed PMID: 26812336.


    Progress 10/01/14 to 09/30/15

    Outputs
    Target Audience:Target audience includes biomedical scientists, veterinarians, animal scientists. This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects came from Federal, State and private funding sources, including biological and pharmaceutical corporations. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Graduate students, veterinary students and post-docs have participated. How have the results been disseminated to communities of interest?Publications, national and international conferences and meetings. Where applicable, outreach activities including veterinary continuing education programs and extension meetings. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

    Impacts
    What was accomplished under these goals? The interdisciplinary research activity in the CVHS centered on focus areas in infectious disease, parasitology, health and performance, and animal models of human disease, provides an underlying infrastructure for basic research into veterinary science and medicine. This infrastructure adds value to our service and teaching missions, and provides a critical resource for Oklahoma in animal and public health.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Liu J, Pope C. The cannabinoid receptor antagonist AM251 increases paraoxon and chlorpyrifos oxon toxicity in rats. Neurotoxicology. 2015 Jan;46:12-8. doi: 10.1016/j.neuro.2014.11.001. Epub 2014 Nov 20. PubMed PMID: 25447325; PubMed Central PMCID: PMC4448943.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Maiti S, Chen G. Ethanol up-regulates phenol sulfotransferase (SULT1A1) and hydroxysteroid sulfotransferase (SULT2A1) in rat liver and intestine. Arch Physiol Biochem. 2015 May;121(2):68-74. doi: 10.3109/13813455.2014.992440. Epub 2015 Feb 27. PubMed PMID: 25720860.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Mani RJ, Metcalf JA, Clinkenbeard KD. Amblyomma americanum as a Bridging Vector for Human Infection with Francisella tularensis. PLoS One. 2015 Jun 29;10(6):e0130513. doi: 10.1371/journal.pone.0130513. eCollection 2015. PubMed PMID: 26121137; PubMed Central PMCID: PMC4486451.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Maples D, McLean K, Sahoo K, Newhardt R, Venkatesan P, Wood B, Ranjan A. Synthesis and characterisation of ultrasound imageable heat-sensitive liposomes for HIFU therapy. Int J Hyperthermia. 2015 Sep;31(6):674-85. doi: 10.3109/02656736.2015.1057622. Epub 2015 Jul 17. PubMed PMID: 26185910.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: McFarlane D, Hill K, Anton J. Neutrophil function in healthy aged horses and horses with pituitary dysfunction. Vet Immunol Immunopathol. 2015 Jun 15;165(3-4):99-106. doi: 10.1016/j.vetimm.2015.04.006. Epub 2015 Apr 30. PubMed PMID: 25962580.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Miller BF, Drake JC, Peelor FF 3rd, Biela LM, Geor R, Hinchcliff K, Davis M, Hamilton KL. Participation in a 1,000-mile race increases the oxidation of carbohydrate in Alaskan sled dogs. J Appl Physiol (1985). 2015 Jun 15;118(12):1502-9. doi: 10.1152/japplphysiol.00588.2014. Epub 2014 Aug 21. PubMed PMID: 25150223.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Miller BF, Ehrlicher SE, Drake JC, Peelor FF 3rd, Biela LM, Pratt-Phillips S, Davis M, Hamilton KL. Assessment of protein synthesis in highly aerobic canine species at the onset and during exercise training. J Appl Physiol (1985). 2015 Apr 1;118(7):811-7. doi: 10.1152/japplphysiol.00982.2014. Epub 2015 Jan 22. PubMed PMID: 25614602.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: More S, Watson A, Stein LE. Bicarotid Trunk in the Domestic Cat. Anat Histol Embryol. 2015 Sep 22. doi: 10.1111/ahe.12202. [Epub ahead of print] PubMed PMID: 26394560.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Nafe LA, Leach SB. Treatment of feline asthma with ciclosporin in a cat with diabetes mellitus and congestive heart failure. J Feline Med Surg. 2014 Dec 19. pii: 1098612X14563342. [Epub ahead of print] PubMed PMID: 25527351.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Narasaraju T, Shukla D, More S, Huang C, Zhang L, Xiao X, Liu L. Role of microRNA-150 and glycoprotein nonmetastatic melanoma protein B in angiogenesis during hyperoxia-induced neonatal lung injury. Am J Respir Cell Mol Biol. 2015 Feb;52(2):253-61. doi: 10.1165/rcmb.2013-0021OC. PubMed PMID: 25054912; PubMed Central PMCID: PMC4370241.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Polf HD, Smith S, Simpson KM, Rochat MC. Nephroureterectomy and ureteroneocystostomy in an alpaca with bilateral ectopic ureters diagnosed by computed tomographic excretory urography. Vet Surg. 2015 Jan;44(1):23-6. doi: 10.1111/j.1532-950X.2014.12211.x. Epub 2014 Jun 2. PubMed PMID: 24889914.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Ponnusamy D, Clinkenbeard KD. Role of Tellurite Resistance Operon in Filamentous Growth of Yersinia pestis in Macrophages. PLoS One. 2015 Nov 4;10(11):e0141984. doi: 10.1371/journal.pone.0141984. eCollection 2015. PubMed PMID: 26536670.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Reichard MV, Criffield M, Thomas JE, Paritte JM, Cunningham M, Onorato D, Logan K, Interisano M, Marucci G, Pozio E. High prevalence of Trichinella pseudospiralis in Florida panthers (Puma concolor coryi). Parasit Vectors. 2015 Feb 4;8:67. doi: 10.1186/s13071-015-0674-z. PubMed PMID: 25649739; PubMed Central PMCID: PMC4324651.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Reppert E, Galindo RC, Ayll�n N, Breshears MA, Kocan KM, Blouin EF, de la Fuente J. Studies of Anaplasma phagocytophilum in sheep experimentally infected with the human NY-18 isolate: characterization of tick feeding sites. Ticks Tick Borne Dis. 2014 Oct;5(6):744-52. doi: 10.1016/j.ttbdis.2014.05.014. Epub 2014 Aug 7. PubMed PMID: 25127160.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Starkey LA, Barrett AW, Beall MJ, Chandrashekar R, Thatcher B, Tyrrell P, Little SE. Persistent Ehrlichia ewingii infection in dogs after natural tick infestation. J Vet Intern Med. 2015 Mar-Apr;29(2):552-5. doi: 10.1111/jvim.12567. Epub 2015 Mar 16. PubMed PMID: 25776536.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Starkey LA, Barrett AW, Chandrashekar R, Stillman BA, Tyrrell P, Thatcher B, Beall MJ, Gruntmeir JM, Meinkoth JH, Little SE. Development of antibodies to and PCR detection of Ehrlichia spp. in dogs following natural tick exposure. Vet Microbiol. 2014 Oct 10;173(3-4):379-84. doi: 10.1016/j.vetmic.2014.08.006. Epub 2014 Aug 15. PubMed PMID: 25213230.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Step DL, Krehbiel C. Evidence-based approach to improving immunity to manage cattle health. Anim Health Res Rev. 2014 Dec;15(2):178-9. doi: 10.1017/S1466252314000206. Epub 2014 Nov 11. PubMed PMID: 25384831.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Velasquez L, Blagburn BL, Duncan-Decoq R, Johnson EM, Allen KE, Meinkoth J, Gruntmeir J, Little SE. Increased prevalence of Dirofilaria immitis antigen in canine samples after heat treatment. Vet Parasitol. 2014 Nov 15;206(1-2):67-70. doi: 10.1016/j.vetpar.2014.03.021. Epub 2014 Mar 28. PubMed PMID: 24785291.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Villar M, Ayll�n N, Kocan KM, Bonz�n-Kulichenko E, Alberdi P, Blouin EF, Weisheit S, Mateos-Hern�ndez L, Cabezas-Cruz A, Bell-Sakyi L, Vancov� M, B�l� T, Meyer DF, Sterba J, Contreras M, Rudenko N, Grubhoffer L, V�zquez J, de la Fuente J. Identification and Characterization of Anaplasma phagocytophilum Proteins Involved in Infection of the Tick Vector, Ixodes scapularis. PLoS One. 2015 Sep 4;10(9):e0137237. doi: 10.1371/journal.pone.0137237. eCollection 2015. PubMed PMID: 26340562; PubMed Central PMCID: PMC4560377.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Shao X, Li J, Wang S, Chen G, Xu J, Ji X, Li L, Lu W, Zhou T. Exogenous dopamine induces dehydroepiandrosterone sulfotransferase (rSULT2A1) in rat liver and changes the pharmacokinetic profile of moxifloxacin in rats. Drug Metab Pharmacokinet. 2015 Feb;30(1):97-104. doi: 10.1016/j.dmpk.2014.10.003. Epub 2014 Nov 6. PubMed PMID: 25760536.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Wu W, Zhang W, More S, Booth JL, Duggan ES, Liu L, Zhao YD, Metcalf JP. Cigarette smoke attenuates the RIG-I-initiated innate antiviral response to influenza infection in two murine models. Am J Physiol Lung Cell Mol Physiol. 2014 Dec 1;307(11):L848-58. doi: 10.1152/ajplung.00158.2014. Epub 2014 Sep 26. PubMed PMID: 25260755; PubMed Central PMCID: PMC4254961.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Zhang L, Huang B, Scherlag BJ, Ritchey JW, Embi AA, Hu J, Hou Y, Po SS. Structural changes in the progression of atrial fibrillation: potential role of glycogen and fibrosis as perpetuating factors. Int J Clin Exp Pathol. 2015 Feb 1;8(2):1712-8. eCollection 2015. PubMed PMID: 25973058; PubMed Central PMCID: PMC4396299.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Ayll�n N, Naranjo V, Hajduaek O, Villar M, Galindo RC, Kocan KM, Alberdi P, `�ma R, Cabezas-Cruz A, R�ckert C, Bell-Sakyi L, Kazim�rov� M, Havl�kov� S, Klempa B, Kop�?ek P, de la Fuente J. Nuclease Tudor-SN Is Involved in Tick dsRNA-Mediated RNA Interference and Feeding but Not in Defense against Flaviviral or Anaplasma phagocytophilum Rickettsial Infection. PLoS One. 2015 Jul 17;10(7):e0133038. doi: 10.1371/journal.pone.0133038. eCollection 2015. PubMed PMID: 26186700; PubMed Central PMCID: PMC4506139.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Ayll�n N, Villar M, Galindo RC, Kocan KM, `�ma R, L�pez JA, V�zquez J, Alberdi P, Cabezas-Cruz A, Kop�?ek P, de la Fuente J. Systems biology of tissue-specific response to Anaplasma phagocytophilum reveals differentiated apoptosis in the tick vector Ixodes scapularis. PLoS Genet. 2015 Mar 27;11(3):e1005120. doi: 10.1371/journal.pgen.1005120. eCollection 2015 Mar. PubMed PMID: 25815810; PubMed Central PMCID: PMC4376793.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Barrett AW, Noden BH, Gruntmeir JM, Holland T, Mitcham JR, Martin JE, Johnson EM, Little SE. County Scale Distribution of Amblyomma americanum (Ixodida: Ixodidae) in Oklahoma: Addressing Local Deficits in Tick Maps Based on Passive Reporting. J Med Entomol. 2015 Mar;52(2):269-73. doi: 10.1093/jme/tju026. Epub 2015 Feb 25. PubMed PMID: 26336311.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Black D, Ritchey J, Payton M, Eberle R. Role of the virion host shutoff protein in neurovirulence of monkey B virus (Macacine herpesvirus 1). Virol Sin. 2014 Oct;29(5):274-83. doi: 10.1007/s12250-014-3495-x. Epub 2014 Oct 22. PubMed PMID: 25341947.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Bordelon JT, Newcomb BT, Rochat MC. Surgical Removal of a Cuterebra Larva from the Cervical Trachea of a Cat. J Am Anim Hosp Assoc. 2014 Nov 21. [Epub ahead of print] PubMed PMID: 25415213.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Brush LA, Black DH, McCormack KA, Maxwell LK, Wright G, Ritchey JW, Payton ME, Eberle R. Papiine herpesvirus 2 as a predictive model for drug sensitivity of Macacine herpesvirus 1 (monkey B virus). Comp Med. 2014 Oct;64(5):386-93. PubMed PMID: 25402179; PubMed Central PMCID: PMC4236787.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Chirkova T, Lin S, Oomens AG, Gaston KA, Boyoglu-Barnum S, Meng J, Stobart CC, Cotton CU, Hartert TV, Moore ML, Ziady AG, Anderson LJ. CX3CR1 is an important surface molecule for respiratory syncytial virus infection in human airway epithelial cells. J Gen Virol. 2015 Sep;96(9):2543-56. doi: 10.1099/vir.0.000218. Epub 2015 Jun 25. PubMed PMID: 26297201.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Cunningham L, Cook A, Hanzlicek A, Harkin K, Wheat J, Goad C, Kirsch E. Sensitivity and Specificity of Histoplasma Antigen Detection by Enzyme Immunoassay. J Am Anim Hosp Assoc. 2015 Sep-Oct;51(5):306-10. doi: 10.5326/JAAHA-MS-6202. PubMed PMID: 26355580.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: de Laat MA, Clement CK, Sillence MN, McGowan CM, Pollitt CC, Lacombe VA. The impact of prolonged hyperinsulinaemia on glucose transport in equine skeletal muscle and digital lamellae. Equine Vet J. 2015 Jul;47(4):494-501. doi: 10.1111/evj.12320. Epub 2014 Sep 10. PubMed PMID: 24995680.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: de Laat MA, Robinson MA, Gruntmeir KJ, Liu Y, Soma LR, Lacombe VA. AICAR administration affects glucose metabolism by upregulating the novel glucose transporter, GLUT8, in equine skeletal muscle. Vet J. 2015 Sep;205(3):381-6. doi: 10.1016/j.tvjl.2015.05.018. Epub 2015 May 27. PubMed PMID: 26116041.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: de Souza PC, Ranjan A, Towner RA. Nanoformulations for therapy of pancreatic and liver cancers. Nanomedicine (Lond). 2015 May;10(9):1515-34. doi: 10.2217/nnm.14.231. PubMed PMID: 25996122.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Drake J, Gruntmeir J, Merritt H, Allen L, Little SE. False negative antigen tests in dogs infected with heartworm and placed on macrocyclic lactone preventives. Parasit Vectors. 2015 Feb 4;8:68. doi: 10.1186/s13071-015-0698-4. PubMed PMID: 25648086; PubMed Central PMCID: PMC4336501.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Drum MG, Marcellin-Little DJ, Davis MS. Principles and applications of therapeutic exercises for small animals. Vet Clin North Am Small Anim Pract. 2015 Jan;45(1):73-90. doi: 10.1016/j.cvsm.2014.09.005. Review. PubMed PMID: 25432682.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Embi AA, Scherlag BJ, Ritchey JW. Glycogen and the propensity for atrial fibrillation: intrinsic anatomic differences in glycogen in the left and right atria in the goat heart. N Am J Med Sci. 2014 Oct;6(10):510-5. doi: 10.4103/1947-2714.143282. PubMed PMID: 25489563; PubMed Central PMCID: PMC4215488.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Fernando R, Maples D, Senavirathna LK, Zheng Y, Polf JC, Benton ER, Bartels KE, Piao D, Ranjan A. Hyperthermia sensitization and proton beam triggered liposomal drug release for targeted tumor therapy. Pharm Res. 2014 Nov;31(11):3120-6. doi: 10.1007/s11095-014-1404-5. Epub 2014 May 23. PubMed PMID: 24852892.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: He C, Medley SC, Hu T, Hinsdale ME, Lupu F, Virmani R, Olson LE. PDGFR? signalling regulates local inflammation and synergizes with hypercholesterolaemia to promote atherosclerosis. Nat Commun. 2015 Jul 17;6:7770. doi: 10.1038/ncomms8770. PubMed PMID: 26183159; PubMed Central PMCID: PMC4507293.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Herndon AM, Breshears MA, McFarlane D. Oxidative modification, inflammation and amyloid in the normal and diabetic cat pancreas. J Comp Pathol. 2014 Nov;151(4):352-62. doi: 10.1016/j.jcpa.2014.06.004. Epub 2014 Aug 11. PubMed PMID: 25124331.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Herrin BH, Zajac AM, Little SE. Confirmation of Borrelia burgdorferi sensu stricto and Anaplasma phagocytophilum in Ixodes scapularis, Southwestern Virginia. Vector Borne Zoonotic Dis. 2014 Nov;14(11):821-3. doi: 10.1089/vbz.2014.1661. PubMed PMID: 25409274.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Holbrook TC, Gilliam LL, Stein FP, Morgan SE, Avery AL, Confer AW, Panciera RJ. Lathyrus hirsutus (Caley Pea) intoxication in a herd of horses. J Vet Intern Med. 2015 Jan;29(1):294-8. doi: 10.1111/jvim.12515. Epub 2015 Jan 16. PubMed PMID: 25594329.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Ji XW, Chen GP, Song Y, Hua M, Wang LJ, Li L, Yuan Y, Wang SY, Zhou TY, Lu W. Intratumoral estrogen sulfotransferase induction contributes to the anti-breast cancer effects of the dithiocarbamate derivative TM208. Acta Pharmacol Sin. 2015 Oct;36(10):1246-55. doi: 10.1038/aps.2015.14. Epub 2015 May 4. PubMed PMID: 25937633.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Jones K, Turner B, Brand�o J, Hubbard SA, Magee D, Baughman B, Wills R, Tully T. Pilot Study: Colostomy and Urine Collection Protocol for Investigating Potential Inciting Causes of Hen Diuresis Syndrome. Avian Dis. 2015 Jun;59(2):227-34. doi: 10.1637/10953-100614-Reg. PubMed PMID: 26473672.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Karikoski NP, Patterson-Kane JC, Singer ER, McFarlane D, McGowan CM. Lamellar pathology in horses with pituitary pars intermedia dysfunction. Equine Vet J. 2015 Apr 13. doi: 10.1111/evj.12450. [Epub ahead of print] PubMed PMID: 25869529.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Lis K, Fern�ndez de Mera IG, Popara M, Cabezas-Cruz A, Ayll�n N, Zweygarth E, Passos LM, Broniszewska M, Villar M, Kocan KM, Ribeiro MF, Pfister K, de la Fuente J. Molecular and immunological characterization of three strains of Anaplasma marginale grown in cultured tick cells. Ticks Tick Borne Dis. 2015 Jun;6(4):522-9. doi: 10.1016/j.ttbdis.2015.04.006. Epub 2015 Apr 24. PubMed PMID: 25943785.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Little SE, Hostetler JA, Thomas JE, Bailey KL, Barrett AW, Gruntmeir K, Gruntmeir J, Starkey LA, Basel C, Blagburn BL. Moxidectin steady state prior to inoculation protects cats from subsequent, repeated infection with Dirofilaria immitis. Parasit Vectors. 2015 Feb 18;8:107. doi: 10.1186/s13071-015-0710-z. PubMed PMID: 25880531; PubMed Central PMCID: PMC4340871.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Liu J, Parsons L, Pope C. Comparative effects of parathion and chlorpyrifos on endocannabinoid and endocannabinoid-like lipid metabolites in rat striatum. Neurotoxicology. 2015 Jul 26;50:20-27. doi: 10.1016/j.neuro.2015.07.006. [Epub ahead of print] PubMed PMID: 26215119.


    Progress 10/01/13 to 09/30/14

    Outputs
    Target Audience: Target audience includes biomedical scientists, veterinarians, animal scientists. This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects came from Federal, State and private funding sources, including biological and pharmaceutical corporations. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Graduate students, veterinary students and post-docs have participated. How have the results been disseminated to communities of interest? Publications, national and international conferences and meetings. Where applicable, outreach activities including veterinary continuing education programs and extension meetings. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

    Impacts
    What was accomplished under these goals? The interdisciplinary research activity in the CVHS centered on focus areas in infectious disease, parasitology, health and performance, and animal models of human disease, provides an underlying infrastructure for basic research into veterinary science and medicine. This infrastructure adds value to our service and teaching missions, and provides a critical resource for Oklahoma in animal and public health.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Achanta S, Maxwell LK. Reaction phenotyping of vinblastine metabolism in dogs. Vet Comp Oncol. 2014 Feb 7. doi: 10.1111/vco.12084. [Epub ahead of print] PubMed PMID: 24502418.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Banse HE, McCann J, Yang F, Wagg C, McFarlane D. Comparison of two methods for measurement of equine insulin. J Vet Diagn Invest. 2014 Jun 13;26(4):527-530. [Epub ahead of print] PubMed PMID: 24928598.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Barrett A, Little SE, Shaw E. "Rickettsia amblyommii" and R. montanensis infection in dogs following natural exposure to ticks. Vector Borne Zoonotic Dis. 2014 Jan;14(1):20-5. doi: 10.1089/vbz.2013.1325. Epub 2013 Dec 20. PubMed PMID: 24359419.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Black D, Ritchey J, Payton M, Eberle R. Role of the virion host shutoff protein in neurovirulence of monkey B virus (Macacine herpesvirus 1). Virol Sin. 2014 Oct;29(5):274-83. doi: 10.1007/s12250-014-3495-x. Epub 2014 Oct 22. PubMed PMID: 25341947.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Black D, Ohsawa K, Tyler S, Maxwell L, Eberle R. A single viral gene determines lethal cross-species neurovirulence of baboon herpesvirus HVP2. Virology. 2014 Mar;452-453:86-94. doi: 10.1016/j.virol.2013.12.038. Epub 2014 Jan 29. PubMed PMID: 24606686; PubMed Central PMCID: PMC3955001.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Brush LA, Black DH, Mccormack KA, Maxwell LK, Wright G, Ritchey JW, Payton ME, Eberle R. Papiine herpesvirus 2 as a predictive model for drug sensitivity of Macacine herpesvirus 1 (monkey B virus). Comp Med. 2014 Oct;64(5):386-93. PubMed PMID: 25402179; PubMed Central PMCID: PMC4236787.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Buczinski S, Rademacher RD, Tripp HM, Edmonds M, Johnson EG, Dufour S. Assessment of l-lactatemia as a predictor of respiratory disease recognition and severity in feedlot steers. Prev Vet Med. 2014 Dec 15. pii: S0167-5877(14)00414-0. doi: 10.1016/j.prevetmed.2014.12.003. [Epub ahead of print] PubMed PMID: 25537763.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Carmichael RC, Duell JR, Holbrook TC, Herrin BH, Leutenegger CM, O'Connor TP, Little SE. Antibodies reactive to Ehrlichia spp. are common in Oklahoma horses. Vector Borne Zoonotic Dis. 2014 Aug;14(8):552-6. doi: 10.1089/vbz.2013.1570. PubMed PMID: 25072984.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Davis MS, Bonen A, Snook LA, Jain SS, Bartels K, Geor R, Hueffer K. Conditioning increases the gain of contraction-induced sarcolemmal substrate transport in ultra-endurance racing sled dogs. PLoS One. 2014 Jul 30;9(7):e103087. doi: 10.1371/journal.pone.0103087. eCollection 2014. PubMed PMID: 25075856; PubMed Central PMCID: PMC4116179.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: de la Fuente J, Villar M, Contreras M, Moreno-Cid JA, Merino O, P�rez de la Lastra JM, de la Fuente G, Galindo RC. Prospects for vaccination against the ticks of pets and the potential impact on pathogen transmission. Vet Parasitol. 2014 Dec 23. pii: S0304-4017(14)00649-9. doi: 10.1016/j.vetpar.2014.12.015. [Epub ahead of print] PubMed PMID: 25555312.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: de Laat MA, Clement CK, McGowan CM, Sillence MN, Pollitt CC, Lacombe VA. Toll-like receptor and pro-inflammatory cytokine expression during prolonged hyperinsulinaemia in horses: implications for laminitis. Vet Immunol Immunopathol. 2014 Jan 15;157(1-2):78-86. doi: 10.1016/j.vetimm.2013.10.010. Epub 2013 Oct 24. PubMed PMID: 24246153.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: de Laat MA, Gruntmeir KJ, Pollitt CC, McGowan CM, Sillence MN, Lacombe VA. Hyperinsulinemia Down-Regulates TLR4 Expression in the Mammalian Heart. Front Endocrinol (Lausanne). 2014 Jul 22;5:120. doi: 10.3389/fendo.2014.00120. eCollection 2014. PubMed PMID: 25101057; PubMed Central PMCID: PMC4105691.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Bourne CR, Wakeham N, Webb N, Nammalwar B, Bunce RA, Berlin KD, Barrow WW. The structure and competitive substrate inhibition of dihydrofolate reductase from Enterococcus faecalis reveal restrictions to cofactor docking. Biochemistry. 2014 Feb 25;53(7):1228-38. doi: 10.1021/bi401104t. Epub 2014 Feb 11. PubMed PMID: 24495113; PubMed Central PMCID: PMC3985486.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Decker JE, McKay SD, Rolf MM, Kim J, Molina Alcal� A, Sonstegard TS, Hanotte O, G�therstr�m A, Seabury CM, Praharani L, Babar ME, Correia de Almeida Regitano L, Yildiz MA, Heaton MP, Liu WS, Lei CZ, Reecy JM, Saif-Ur-Rehman M, Schnabel RD, Taylor JF. Worldwide patterns of ancestry, divergence, and admixture in domesticated cattle. PLoS Genet. 2014 Mar 27;10(3):e1004254. doi: 10.1371/journal.pgen.1004254. eCollection 2014 Mar. PubMed PMID: 24675901; PubMed Central PMCID: PMC3967955.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Drum MG, Marcellin-Little DJ, Davis MS. Principles and applications of therapeutic exercises for small animals. Vet Clin North Am Small Anim Pract. 2015 Jan;45(1):73-90. doi: 10.1016/j.cvsm.2014.09.005. PubMed PMID: 25432682.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Duscher GG, Galindo RC, Tichy A, Hummel K, Kocan KM, de la Fuente J. Glutathione S-transferase affects permethrin detoxification in the brown dog tick, Rhipicephalus sanguineus. Ticks Tick Borne Dis. 2014 Apr;5(3):225-33. doi: 10.1016/j.ttbdis.2013.11.006. Epub 2014 Feb 16. PubMed PMID: 24548526.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Embi AA, Scherlag BJ, Ritchey JW. Glycogen and the propensity for atrial fibrillation: intrinsic anatomic differences in glycogen in the left and right atria in the goat heart. N Am J Med Sci. 2014 Oct;6(10):510-5. doi: 10.4103/1947-2714.143282. PubMed PMID: 25489563; PubMed Central PMCID: PMC4215488.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Fernando R, Maples D, Senavirathna LK, Zheng Y, Polf JC, Benton ER, Bartels KE, Piao D, Ranjan A. Hyperthermia sensitization and proton beam triggered liposomal drug release for targeted tumor therapy. Pharm Res. 2014 Nov;31(11):3120-6. doi: 10.1007/s11095-014-1404-5. Epub 2014 May 23. PubMed PMID: 24852892.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Fulton RW, d'Offay JM, Eberle R, Moeller RB, Campen HV, O'Toole D, Chase C, Miller MM, Sprowls R, Nydam DV. Bovine herpesvirus-1: Evaluation of genetic diversity of subtypes derived from field strains of varied clinical syndromes and their relationship to vaccine strains. Vaccine. 2015 Jan 15;33(4):549-58. doi: 10.1016/j.vaccine.2014.11.033. Epub 2014 Nov 30. PubMed PMID: 25454086.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Fulton RW, Herd HR, Sorensen NJ, Confer AW, Ritchey JW, Ridpath JF, Burge LJ. Enteric disease in postweaned beef calves associated with Bovine coronavirus clade 2. J Vet Diagn Invest. 2015 Jan;27(1):97-101. doi: 10.1177/1040638714559026. Epub 2014 Nov 26. PubMed PMID: 25428188.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Guo Y, Mishra A, Weng T, Chintagari NR, Wang Y, Zhao C, Huang C, Liu L. Wnt3a mitigates acute lung injury by reducing P2X7 receptor-mediated alveolar epithelial type I cell death. Cell Death Dis. 2014 Jun 12;5:e1286. doi: 10.1038/cddis.2014.254. PubMed PMID: 24922070.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Herndon AM, Breshears MA, McFarlane D. Oxidative modification, inflammation and amyloid in the normal and diabetic cat pancreas. J Comp Pathol. 2014 Nov;151(4):352-62. doi: 10.1016/j.jcpa.2014.06.004. Epub 2014 Aug 11. PubMed PMID: 25124331.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Herrin BH, Zajac AM, Little SE. Confirmation of Borrelia burgdorferi sensu stricto and Anaplasma phagocytophilum in Ixodes scapularis, Southwestern Virginia. Vector Borne Zoonotic Dis. 2014 Nov;14(11):821-3. doi: 10.1089/vbz.2014.1661. PubMed PMID: 25409274.
    • Type: Journal Articles Status: Published Year Published: 2015 Citation: Holbrook TC, Gilliam LL, Stein FP, Morgan SE, Avery AL, Confer AW, Panciera RJ. Lathyrus hirsutus (Caley Pea) Intoxication in a Herd of Horses. J Vet Intern Med. 2015 Jan 16. doi: 10.1111/jvim.12515. [Epub ahead of print] PubMed PMID: 25594329.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Huang C, Zhou T, Chen Y, Sun T, Zhang S, Chen G. Estrogen-related receptor ERR? regulation of human hydroxysteroid sulfotransferase (SULT2A1) gene expression in human Caco-2 cells. J Biochem Mol Toxicol. 2014 Jan;28(1):32-8. doi: 10.1002/jbt.21520. Epub 2013 Sep 13. PubMed PMID: 24038886.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Lacombe VA. Expression and regulation of facilitative glucose transporters in equine insulin-sensitive tissue: from physiology to pathology. ISRN Vet Sci. 2014 Mar 4;2014:409547. doi: 10.1155/2014/409547. eCollection 2014. Review. PubMed PMID: 24977043; PubMed Central PMCID: PMC4060548.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Layman QD, Rezabek GB, Ramachandran A, Love BC, Confer AW. A retrospective study of equine actinobacillosis cases: 1999-2011. J Vet Diagn Invest. 2014 Apr 17;26(3):365-375. [Epub ahead of print] PubMed PMID: 24742921.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Little SE, Munzing C, Heise SR, Allen KE, Starkey LA, Johnson EM, Meinkoth J, Reichard MV. Pre-treatment with heat facilitates detection of antigen of Dirofilaria immitis in canine samples. Vet Parasitol. 2014 Jun 16;203(1-2):250-2. doi: 10.1016/j.vetpar.2014.01.007. Epub 2014 Jan 18. PubMed PMID: 24576603.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Lee CD, Maxwell LK. Effect of body weight on the pharmacokinetics of flunixin meglumine in miniature horses and quarter horses. J Vet Pharmacol Ther. 2014 Feb;37(1):35-42. doi: 10.1111/jvp.12056. Epub 2013 May 10. PubMed PMID: 23659780.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Little SE, Beall MJ, Bowman DD, Chandrashekar R, Stamaris J. Canine infection with Dirofilaria immitis, Borrelia burgdorferi, Anaplasma spp., and Ehrlichia spp. in the United States, 2010-2012. Parasit Vectors. 2014 May 30;7:257. doi:10.1186/1756-3305-7-257. PubMed PMID: 24886589; PubMed Central PMCID: PMC4057565.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Little SE, Raymond MR, Thomas JE, Gruntmeir J, Hostetler JA, Meinkoth JH, Blagburn BL. Heat treatment prior to testing allows detection of antigen of Dirofilaria immitis in feline serum. Parasit Vectors. 2014 Jan 13;7:1. doi: 10.1186/1756-3305-7-1. PubMed PMID: 24411014; PubMed Central PMCID: PMC3895812.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Liu J, Pope C. The cannabinoid receptor antagonist AM251 increases paraoxon and chlorpyrifos oxon toxicity in rats. Neurotoxicology. 2014 Nov 20;46C:12-18. doi: 10.1016/j.neuro.2014.11.001. [Epub ahead of print] PubMed PMID: 25447325.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Liu XY, de la Fuente J, Cote M, Galindo RC, Moutailler S, Vayssier-Taussat M, Bonnet SI. IrSPI, a tick serine protease inhibitor involved in tick feeding and Bartonella henselae infection. PLoS Negl Trop Dis. 2014 Jul 24;8(7):e2993. doi: 10.1371/journal.pntd.0002993. eCollection 2014 Jul. PubMed PMID: 25057911; PubMed Central PMCID: PMC4109860.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Maxwell CL, Krehbiel CR, Wilson BK, Johnson BT, Bernhard BC, O'Neill CF, VanOverbeke DL, Mafi GG, Step DL, Richards CJ. Effects of beef production system on animal performance and carcass characteristics. J Anim Sci. 2014 Dec;92(12):5727-38. doi: 10.2527/jas.2014-7639. Epub 2014 Nov 17. PubMed PMID: 25403195.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Miller BF, Drake JC, Peelor FF 3rd, Biela LM, Geor RJ, Hinchcliff KW, Davis M, Hamilton KL. Participation in a 1000-mile race increases the oxidation of carbohydrate in Alaskan sled dogs. J Appl Physiol (1985). 2014 Aug 21:jap.00588.2014. doi: 10.1152/japplphysiol.00588.2014. [Epub ahead of print] PubMed PMID: 25150223.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Nafe LA, Leach SB. Treatment of feline asthma with ciclosporin in a cat with diabetes mellitus and congestive heart failure. J Feline Med Surg. 2014 Dec 19. pii: 1098612X14563342. [Epub ahead of print] PubMed PMID: 25527351.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Reppert E, Galindo RC, Ayll�n N, Breshears MA, Kocan KM, Blouin EF, de la Fuente J. Studies of Anaplasma phagocytophilum in sheep experimentally infected with the human NY-18 isolate: characterization of tick feeding sites. Ticks Tick Borne Dis. 2014 Oct;5(6):744-52. doi: 10.1016/j.ttbdis.2014.05.014. Epub 2014 Aug 7. PubMed PMID: 25127160.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Nammalwar B, Bourne CR, Wakeham N, Bourne PC, Barrow EW, Muddala NP, Bunce RA, Berlin KD, Barrow WW. Modified 2,4-diaminopyrimidine-based dihydrofolate reductase inhibitors as potential drug scaffolds against Bacillus anthracis. Bioorg Med Chem. 2015 Jan 1;23(1):203-11. doi: 10.1016/j.bmc.2014.11.009. Epub 2014 Nov 11. PubMed PMID: 25435253; PubMed Central PMCID: PMC4278362.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Nammalwar B, Muddala NP, Bourne CR, Henry M, Bourne PC, Bunce RA, Barrow EW, Berlin KD, Barrow WW. Synthesis and biological evaluation of 2,4-diaminopyrimidine-based antifolate drugs against Bacillus anthracis. Molecules. 2014 Mar 17;19(3):3231-46. doi: 10.3390/molecules19033231. PubMed PMID: 24642909; PubMed Central PMCID: PMC4016962.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Pucket JD, Boileau MJ, Sula MJ. Calcific band keratopathy in an alpaca. Vet Ophthalmol. 2014 Jul;17(4):286-9. doi: 10.1111/vop.12097. Epub 2013 Sep 3. PubMed PMID: 23998709.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Shakya M, Kumar B, Nagar G, de la Fuente J, Ghosh S. Subolesin: a candidate vaccine antigen for the control of cattle tick infestations in Indian situation. Vaccine. 2014 Jun 12;32(28):3488-94. doi: 10.1016/j.vaccine.2014.04.053. Epub 2014 Apr 30. PubMed PMID: 24795229.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Silva JB, Cabezas-Cruz A, Fonseca AH, Barbosa JD, de la Fuente J. Infection of water buffalo in Rio de Janeiro Brazil with Anaplasma marginale strains also reported in cattle. Vet Parasitol. 2014 Oct 15;205(3-4):730-4. doi: 10.1016/j.vetpar.2014.09.009. Epub 2014 Sep 19. PubMed PMID: 25260335.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Simpson KM, Streeter RN, Taylor JD, Gull TB, Step DL. Bacteremia in the pedal circulation following regional intravenous perfusion of a 2% lidocaine solution in cattle with deep digital sepsis. J Am Vet Med Assoc. 2014 Sep 1;245(5):565-70. doi: 10.2460/javma.245.5.565. PubMed PMID: 25148098.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Smith KM, Maxwell L, Gull T, Payton ME, Gilmour MA. Stability of 1% voriconazole solution in a constant-rate infusion pump for topical ocular delivery to horses. Vet Ophthalmol. 2014 Jul;17 Suppl 1:82-9. doi: 10.1111/vop.12155. Epub 2014 Mar 7. PubMed PMID: 24602272.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Stoffel RT, McClure JC, Butcher MM, Johnson GC, Roland W, Cheng C, Sirigireddy KR, Ganta R, Boughan K, Ewing SA, Stich RW. Experimental infection of Rhipicephalus sanguineus with Ehrlichia chaffeensis. Vet Microbiol. 2014 Aug 6;172(1-2):334-8. doi: 10.1016/j.vetmic.2014.04.017. Epub 2014 May 4. PubMed PMID: 24894131; PubMed Central PMCID: PMC4098862.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Smith KM, Pucket JD, Gilmour MA. Treatment of six cases of equine corneal stromal abscessation with intracorneal injection of 5% voriconazole solution. Vet Ophthalmol. 2014 Jul;17 Suppl 1:179-85. doi: 10.1111/vop.12136. Epub 2014 Jan 6. PubMed PMID: 24387183.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Snider TA, Gull T, Jackson TA, Martinez-Becerra FJ, Picking DR, Picking WD, Picking WL. Experimental salmonellosis challenge model in older calves. Vet Microbiol. 2014 May 14;170(1-2):65-72. doi: 10.1016/j.vetmic.2013.11.018. Epub 2013 Nov 28. PubMed PMID: 24529790.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Step DL, Krehbiel C. Evidence-based approach to improving immunity to manage cattle health. Anim Health Res Rev. 2014 Dec;15(2):178-9. doi: 10.1017/S1466252314000206. Epub 2014 Nov 11. PubMed PMID: 25384831.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Sula MM, Njaa BL, Payton ME. Histologic characterization of the cat middle ear: in sickness and in health. Vet Pathol. 2014 Sep;51(5):951-67. doi: 10.1177/0300985813511125. Epub 2013 Nov 26. PubMed PMID: 24280942.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Taylor JD, Doyle DJ, Blackall PJ, Confer AW. Use of REP-PCR and 16S rRNA gene sequencing for comparison of Mannheimia haemolytica isolates obtained from fatal cases of bovine respiratory disease in the USA and Australia. Aust Vet J. 2014 Jan;92(1-2):15-23. doi: 10.1111/avj.12137. PubMed PMID: 24471877.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Velasquez L, Blagburn BL, Duncan-Decoq R, Johnson EM, Allen KE, Meinkoth J, Gruntmeir J, Little SE. Increased prevalence of Dirofilaria immitis antigen in canine samples after heat treatment. Vet Parasitol. 2014 Nov 15;206(1-2):67-70. doi: 10.1016/j.vetpar.2014.03.021. Epub 2014 Mar 28. PubMed PMID: 24785291.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Villar M, Popara M, Ayll�n N, Fern�ndez de Mera IG, Mateos-Hern�ndez L, Galindo RC, Manrique M, Tobes R, de la Fuente J. A systems biology approach to the characterization of stress response in Dermacentor reticulatus tick unfed larvae. PLoS One. 2014 Feb 21;9(2):e89564. doi: 10.1371/journal.pone.0089564. eCollection 2014. PubMed PMID: 24586875; PubMed Central PMCID: PMC3931811.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Walker JT, Rochat MC, Snider TA, Payton ME. The relevance of threaded external skeletal fixation pin insertion speed in canine bone with and without predrilling. Vet Comp Orthop Traumatol. 2014;27(4):249-56. doi: 10.3415/VCOT-14-01-0009. Epub 2014 Jul 3. PubMed PMID: 24992079.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Starkey LA, Barrett AW, Chandrashekar R, Stillman BA, Tyrrell P, Thatcher B, Beall MJ, Gruntmeir JM, Meinkoth JH, Little SE. Development of antibodies to and PCR detection of Ehrlichia spp. in dogs following natural tick exposure. Vet Microbiol. 2014 Oct 10;173(3-4):379-84. doi: 10.1016/j.vetmic.2014.08.006. Epub 2014 Aug 15. PubMed PMID: 25213230.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Wu W, Zhang W, More S, Booth JL, Duggan ES, Liu L, Zhao YD, Metcalf JP. Cigarette smoke attenuates the RIG-I-initiated innate antiviral response to influenza infection in two murine models. Am J Physiol Lung Cell Mol Physiol. 2014 Dec 1;307(11):L848-58. doi: 10.1152/ajplung.00158.2014. Epub 2014 Sep 26. PubMed PMID: 25260755; PubMed Central PMCID: PMC4254961.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Willis EL, Wolf RF, White GL, McFarlane D. Age- and gender-associated changes in the concentrations of serum TGF-1?, DHEA-S and IGF-1 in healthy captive baboons (Papio hamadryas anubis). Gen Comp Endocrinol. 2014 Jan 1;195:21-7. doi: 10.1016/j.ygcen.2013.10.004. Epub 2013 Oct 23. PubMed PMID: 24161750; PubMed Central PMCID: PMC3888644.
    • Type: Journal Articles Status: Published Year Published: 2014 Citation: Willis EL, Eberle R, Wolf RF, White GL, McFarlane D. The effects of age and cytomegalovirus on markers of inflammation and lymphocyte populations in captive baboons. PLoS One. 2014 Sep 22;9(9):e107167. doi: 10.1371/journal.pone.0107167. eCollection 2014. PubMed PMID: 25244034; PubMed Central PMCID: PMC4170980.


    Progress 10/01/12 to 09/30/13

    Outputs
    Target Audience: This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non­USDA projects came from Federal, State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? These research opportunities provide training for graduate students, DVM trainees in clinical and pathology residency programs, and veterinary students during the summer and in a Research Elective course during the fall and spring semesters. In 2013, there were 34 students enrolled in graduate degree programs, 10 of these were also in post-DVM residency training; 12 veterinary students participated in research projects in the summer; 7 veterinary students enrolled in Research Elective in the spring and 4 enrolled in the fall. How have the results been disseminated to communities of interest? Publications in scientific journals. What do you plan to do during the next reporting period to accomplish the goals? Continue to pursue the goals outlined.

    Impacts
    What was accomplished under these goals? The interdisciplinary research activity in the CVHS centered on focus areas in infectious disease, parasitology, health and performance, and animal models of human disease, provides an underlying infrastructure for basic research into veterinary science and medicine. This infrastructure adds value to our service and teaching missions, and provides a critical resource for Oklahoma in animal and public health. We continue to expand this infrastructure with a new $11 million grant from NIH for respiratory disease research.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Achanta S, Ngo M, Veitenheimer A, Maxwell LK, Wagner JR. Simultaneous quantification of vinblastine and desacetylvinblastine concentrations in canine plasma and urine samples using LC-APCI-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jan 15;913-914:147-54. doi: 10.1016/j.jchromb.2012.11.012. Epub 2012 Dec 20. PubMed PMID: 23314352.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Allison RW, Little SE. Diagnosis of rickettsial diseases in dogs and cats. Vet Clin Pathol. 2013 Jun;42(2):127-44. doi: 10.1111/vcp.12040. Epub 2013 Apr 23. PubMed PMID: 23647393.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Baviskar PS, Hotard AL, Moore ML, Oomens AG. The respiratory syncytial virus fusion protein targets to the perimeter of inclusion bodies and facilitates filament formation by a cytoplasmic tail-dependent mechanism. J Virol. 2013 Oct;87(19):10730-41. doi: 10.1128/JVI.03086-12. Epub 2013 Jul 31. PubMed PMID: 23903836; PubMed Central PMCID: PMC3807408.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Bourne CR, Wakeham N, Nammalwar B, Tseitin V, Bourne PC, Barrow EW, Mylvaganam S, Ramnarayan K, Bunce RA, Berlin KD, Barrow WW. Structure-activity relationship for enantiomers of potent inhibitors of B. anthracis dihydrofolate reductase. Biochim Biophys Acta. 2013 Jan;1834(1):46-52. doi: 10.1016/j.bbapap.2012.09.001. Epub 2012 Sep 20. PubMed PMID: 22999981; PubMed Central PMCID: PMC3530638.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Carmichael RJ, Whitfield C, Maxwell LK. Pharmacokinetics of ganciclovir and valganciclovir in the adult horse. J Vet Pharmacol Ther. 2013 Oct;36(5):441-9. doi: 10.1111/jvp.12029. Epub 2013 Jan 10. PubMed PMID: 23301502.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Chen Y, Zhang S, Zhou T, Huang C, McLaughlin A, Chen G. Liver X receptor alpha mediated genistein induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1) in Hep G2 cells. Toxicol Appl Pharmacol. 2013 Apr 15;268(2):106-12. doi: 10.1016/j.taap.2013.01.006. Epub 2013 Jan 23. PubMed PMID: 23352501; PubMed Central PMCID: PMC3604154.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: de Laat MA, Clement CK, McGowan CM, Sillence MN, Pollitt CC, Lacombe VA. Toll-like receptor and pro-inflammatory cytokine expression during prolonged hyperinsulinaemia in horses: Implications for laminitis. Vet Immunol Immunopathol. 2013 Oct 24. doi:pii: S0165-2427(13)00279-1. 10.1016/j.vetimm.2013.10.010. [Epub ahead of print] PubMed PMID: 24246153.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: d'Offay JM, Eberle R, Wolf RF, Kosanke SD, Doocy KR, Ayalew S, Mansfeild KG, White GL. Simian T-lymphotropic Virus-associated lymphoma in 2 naturally infected baboons: T-cell clonal expansion and immune response during tumor development. Comp Med. 2013 Jun;63(3):288-94. PubMed PMID: 23759532; PubMed Central PMCID: PMC3690435.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: d'Offay JM, Fulton RW, Eberle R. Complete genome sequence of the NVSL BoHV-1.1 Cooper reference strain. Arch Virol. 2013 May;158(5):1109-13. doi: 10.1007/s00705-012-1574-6. Epub 2012 Dec 20. PubMed PMID: 23254967.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Fernando R, Downs J, Maples D, Ranjan A. MRI-guided monitoring of thermal dose and targeted drug delivery for cancer therapy. Pharm Res. 2013 Nov;30(11):2709-17. doi: 10.1007/s11095-013-1110-8. Epub 2013 Jun 19. PubMed PMID: 23780716.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Fulton RW, d'Offay JM, Eberle R. Bovine herpesvirus-1: comparison and differentiation of vaccine and field strains based on genomic sequence variation. Vaccine. 2013 Mar 1;31(11):1471-9. doi: 10.1016/j.vaccine.2013.01.013. Epub 2013 Jan 16. PubMed PMID: 23333211.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Gilliam LL, Carmichael RC, Holbrook TC, Taylor JM, Ownby CL, McFarlane D, Payton ME. Antibody responses to natural rattlesnake envenomation and a rattlesnake toxoid vaccine in horses. Clin Vaccine Immunol. 2013 May;20(5):732-7. doi: 10.1128/CVI.00004-13. Epub 2013 Mar 20. PubMed PMID: 23515015; PubMed Central PMCID: PMC3647753.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Gilliam LL, Ownby CL, McFarlane D, Canida A, Holbrook TC, Payton ME, Krehbiel CR. Development of a double sandwich fluorescent ELISA to detect rattlesnake venom in biological samples from horses with a clinical diagnosis of rattlesnake bite. Toxicon. 2013 Oct;73:63-8. doi: 10.1016/j.toxicon.2013.06.022. Epub 2013 Jul 5. PubMed PMID: 23834918.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Huang C, Zhou T, Chen Y, Sun T, Zhang S, Chen G. Estrogen-Related Receptor ERR? Regulation of Human Hydroxysteroid Sulfotransferase (SULT2A1) Gene Expression in Human Caco-2 Cells. J Biochem Mol Toxicol. 2013 Sep 13. doi: 10.1002/jbt.21520. [Epub ahead of print] PubMed PMID: 24038886.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Lacombe VA, Mayes M, Mosseri S, Reed SM, Ou TH. Distribution and predictive factors of seizure types in 104 cases. Equine Vet J. 2013 Jul 26. doi: 10.1111/evj.12149. [Epub ahead of print] PubMed PMID: 23888910.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Fulton RW, Ridpath JF, Burge LJ. Bovine coronaviruses from the respiratory tract: antigenic and genetic diversity. Vaccine. 2013 Jan 30;31(6):886-92. doi: 10.1016/j.vaccine.2012.12.006. Epub 2012 Dec 14. PubMed PMID: 23246548.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Fulton RW. Host response to bovine viral diarrhea virus and interactions with infectious agents in the feedlot and breeding herd. Biologicals. 2013 Jan;41(1):31-8. doi: 10.1016/j.biologicals.2012.07.009. Epub 2012 Aug 11. PubMed PMID: 22890128.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Lee CD, Maxwell LK. Effect of body weight on the pharmacokinetics of flunixin meglumine in miniature horses and quarter horses. J Vet Pharmacol Ther. 2013 May 10. doi: 10.1111/jvp.12056. [Epub ahead of print] PubMed PMID: 23659780.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Little SE. Future challenges for parasitology: vector control and one health in the Americas. Vet Parasitol. 2013 Aug 1;195(3-4):249-55. doi: 10.1016/j.vetpar.2013.04.006. Epub 2013 Apr 6. PubMed PMID: 23623185.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Liu J, Parsons L, Pope C. Comparative effects of parathion and chlorpyrifos on extracellular endocannabinoid levels in rat hippocampus: influence on cholinergic toxicity. Toxicol Appl Pharmacol. 2013 Nov 1;272(3):608-15. doi: 10.1016/j.taap.2013.07.025. Epub 2013 Aug 7. PubMed PMID: 23933531; PubMed Central PMCID: PMC3831619.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Naikare H, Butcher J, Flint A, Xu J, Raymond KN, Stintzi A. Campylobacter jejuni ferric-enterobactin receptor CfrA is TonB3 dependent and mediates iron acquisition from structurally different catechol siderophores. Metallomics. 2013 Aug;5(8):988-96. doi: 10.1039/c3mt20254b. PubMed PMID: 23702883; PubMed Central PMCID: PMC3754778.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Naranjo V, Ayll�n N, P�rez de la Lastra JM, Galindo RC, Kocan KM, Blouin EF, Mitra R, Alberdi P, Villar M, de la Fuente J. Reciprocal regulation of NF-kB (Relish) and Subolesin in the tick vector, Ixodes scapularis. PLoS One. 2013 Jun 12;8(6):e65915. doi: 10.1371/journal.pone.0065915. Print 2013. PubMed PMID: 23776567; PubMed Central PMCID: PMC3680474.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Reichard MV, Thomas JE, Arther RG, Hostetler JA, Raetzel KL, Meinkoth JH, Little SE. Efficacy of an imidacloprid 10 % / flumethrin 4.5 % collar (Seresto�, Bayer) for preventing the transmission of Cytauxzoon felis to domestic cats by Amblyomma americanum. Parasitol Res. 2013 Aug;112 Suppl 1:11-20. doi: 10.1007/s00436-013-3277-7. PubMed PMID: 23760871.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Senavirathna LK, Fernando R, Maples D, Zheng Y, Polf JC, Ranjan A. Tumor Spheroids as an in vitro model for determining the therapeutic response to proton beam radiotherapy and thermally sensitive nanocarriers. Theranostics. 2013 Aug 21;3(9):687-91. doi: 10.7150/thno.6381. PubMed PMID: 24019853; PubMed Central PMCID: PMC3767115.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Smith SE, Devine DV. Hand-assisted laparoscopic ovariectomy and colpotomy in standing mares. Vet Surg. 2013 Jun;42(5):586-90. doi: 10.1111/j.1532-950X.2012.01007.x. Epub 2013 Jan 8. PubMed PMID: 23298267.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Wang Y, Huang C, Reddy Chintagari N, Bhaskaran M, Weng T, Guo Y, Xiao X, Liu L. miR-375 regulates rat alveolar epithelial cell trans-differentiation by inhibiting Wnt/?-catenin pathway. Nucleic Acids Res. 2013 Apr 1;41(6):3833-44. doi: 10.1093/nar/gks1460. Epub 2013 Feb 8. PubMed PMID: 23396279; PubMed Central PMCID: PMC3616718.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Willis EL, Wolf RF, White GL, McFarlane D. Age- and gender-associated changes in the concentrations of serum TGF-1?, DHEA-S and IGF-1 in healthy captive baboons (Papio hamadryas anubis). Gen Comp Endocrinol. 2013 Oct 23;195C:21-27. doi: 10.1016/j.ygcen.2013.10.004. [Epub ahead of print] PubMed PMID: 24161750.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Wood J, Johnson EM, Allen KE, Campbell GA, Rezabek G, Bradway DS, Pittman LL, Little SE, Panciera RJ. Merogonic stages of Theileria cervi in mule deer (Odocoileus hemionus). J Vet Diagn Invest. 2013 Sep;25(5):662-5. doi: 10.1177/1040638713501173. PubMed PMID: 24029405.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Starkey LA, West MD, Barrett AW, Saucier JM, O'Connor TP, Paras KL, Reiskind MH, Reichard MV, Little SE. Prevalence of antibodies to spotted fever group Rickettsia spp. and Ehrlichia spp. in coyotes (Canis latrans) in Oklahoma and Texas, USA. J Wildl Dis. 2013 Jul;49(3):670-3. doi: 10.7589/2012-08-215. PubMed PMID: 23778619.
    • Type: Journal Articles Status: Published Year Published: 2013 Citation: Sula MJ, Morgan S, Bailey KL, Schumpert M, Njaa BL. Characterization of cardiac lesions in calves after ingestion of Japanese yew (Taxus cuspidata). J Vet Diagn Invest. 2013 Jul;25(4):522-6. doi: 10.1177/1040638713489981. Epub 2013 Jun 18. PubMed PMID: 23780931.


    Progress 10/01/11 to 09/30/12

    Outputs
    OUTPUTS: This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these nonUSDA projects came from Federal, State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

    Impacts
    The interdisciplinary research activity in the CVHS centered on focus areas in infectious disease, parasitology, health and performance, and animal models of human disease, provides an underlying infrastructure for basic research into veterinary science and medicine. This infrastructure adds value to our service and teaching missions, and provides a critical resource for Oklahoma in animal and public health.

    Publications

    • Sula MJ, Winslow CM, Boileau MJ, Barker LD, Panciera RJ. Heat-related injury in lambs. J Vet Diagn Invest. 2012 Jul;24(4):772-6. Epub 2012 May 14. PubMed PMID: 22585957.
    • Weng T, Mishra A, Guo Y, Wang Y, Su L, Huang C, Zhao C, Xiao X, Liu L. Regulation of lung surfactant secretion by microRNA-150. Biochem Biophys Res Commun. 2012 Jun 15;422(4):586-9. Epub 2012 May 14. PubMed PMID: 22595456; PubMed Central PMCID: PMC3377846.
    • Zhao C, Huang C, Weng T, Xiao X, Ma H, Liu L. Computational prediction of MicroRNAs targeting GABA receptors and experimental verification of miR-181, miR-216 and miR-203 targets in GABA-A receptor. BMC Res Notes. 2012 Feb 9;5:91. doi: 10.1186/1756-0500-5-91. PubMed PMID: 22321448; PubMed Central PMCID: PMC3296612.
    • Barrow EW, Clinkenbeard PA, Duncan-Decocq RA, Perteet RF, Hill KD, Bourne PC, Valderas MW, Bourne CR, Clarkson NL, Clinkenbeard KD, Barrow WW. High-throughput screening of a diversity collection using biodefense category A and B priority pathogens. J Biomol Screen. 2012 Aug;17(7):946-56. Epub 2012 May 31. PubMed PMID: 22653912.
    • Bhaskaran M, Xi D, Wang Y, Huang C, Narasaraju T, Shu W, Zhao C, Xiao X, More S, Breshears M, Liu L. Identification of microRNAs changed in the neonatal lungs in response to hyperoxia exposure. Physiol Genomics. 2012 Oct 17;44(20):970-80. doi: 10.1152/physiolgenomics.00145.2011. Epub 2012 Aug 21. PubMed PMID: 22911455; PubMed Central PMCID: PMC3472467.
    • Bourne CR, Wakeham N, Bunce RA, Nammalwar B, Berlin KD, Barrow WW. Classifying compound mechanism of action for linking whole cell phenotypes to molecular targets. J Mol Recognit. 2012 Apr;25(4):216-23. doi: 10.1002/jmr.2174. PubMed PMID: 22434711.
    • Bourne CR, Wakeham N, Nammalwar B, Tseitin V, Bourne PC, Barrow EW, Mylvaganam S, Ramnarayan K, Bunce RA, Berlin KD, Barrow WW. Structure-activity relationship for enantiomers of potent inhibitors of B. anthracis dihydrofolate reductase. Biochim Biophys Acta. 2012 Sep 20. doi:pii: S1570-9639(12)00205-1. 10.1016/j.bbapap.2012.09.001. [Epub ahead of print] PubMed PMID: 22999981.
    • Chintagari NR, Liu L. GABA receptor ameliorates ventilator-induced lung injury in rats by improving alveolar fluid clearance. Crit Care. 2012 Apr 5;16(2):R55. [Epub ahead of print] PubMed PMID: 22480160.
    • Confer AW, Ayalew S. The OmpA family of proteins: Roles in bacterial pathogenesis and immunity. Vet Microbiol. 2012 Aug 31. doi:pii: S0378-1135(12)00483-X. 10.1016/j.vetmic.2012.08.019. [Epub ahead of print] PubMed PMID: 22986056.
    • Cordero M, Brorsen BW, McFarlane D. Circadian and circannual rhythms of cortisol, ACTH, and α-melanocyte-stimulating hormone in healthy horses. Domest Anim Endocrinol. 2012 Nov;43(4):317-24. doi: 10.1016/j.domaniend.2012.05.005. Epub 2012 May 27. PubMed PMID: 22717182.
    • Fulton RW. Host response to bovine viral diarrhea virus and interactions with infectious agents in the feedlot and breeding herd. Biologicals. 2012 Aug 10. [Epub ahead of print] PubMed PMID: 22890128.
    • Gilmour MA, Payton ME. Comparison of the effects of IV administration of meloxicam, carprofen, and flunixin meglumine on prostaglandin E(2) concentration in aqueous humor of dogs with aqueocentesis-induced anterior uveitis. Am J Vet Res. 2012 May;73(5):698-703. PubMed PMID: 22533402.
    • Hanzlicek AS, Ganta C, Myers CB, Grauer GF. Renal transitional-cell carcinoma in two cats with chronic kidney disease. J Feline Med Surg. 2012 Apr;14(4):280-4. Epub 2012 Jan 23. PubMed PMID: 22269336.
    • Hanzlicek AS, Roof CJ, Sanderson MW, Grauer GF. Comparison of urine dipstick, sulfosalicylic acid, urine protein-to-creatinine ratio and a feline-specific immunoassay for detection of albuminuria in cats with chronic kidney disease. J Feline Med Surg. 2012 Jul 16. [Epub ahead of print] PubMed PMID: 22802340.
    • Kocan KM, Busby AT, Allison RW, Breshears MA, Coburn L, Galindo RC, Ayllon N, Blouin EF, de la Fuente J. Sheep experimentally infected with a human isolate of Anaplasma phagocytophilum serve as a host for infection of Ixodes scapularis ticks. Ticks Tick Borne Dis. 2012 Jun;3(3):147-53. Epub 2012 Apr 23. PubMed PMID: 22534515.
    • Lane LV, Allison RW, Rizzi TR, Stern AW, Snider TA, Moore PF, Vernau W. Canine intravascular lymphoma with overt leukemia. Vet Clin Pathol. 2012 Mar;41(1):84-91. doi: 10.1111/j.1939-165X.2011.00399.x. Epub 2012 Jan 19. PubMed PMID: 22260096.
    • Mani RJ, Reichard MV, Morton RJ, Kocan KM, Clinkenbeard KD. Biology of Francisella tularensis subspecies holarctica live vaccine strain in the tick vector Dermacentor variabilis. PLoS One. 2012;7(4):e35441. Epub 2012 Apr 18. PubMed PMID: 22530023; PubMed Central PMCID: PMC3329428.
    • McFarlane D, Wolf RF, McDaniel KA, White GL. The effect of season on inflammatory response in captive baboons. J Med Primatol. 2012 Dec;41(6):341-8. doi: 10.1111/j.1600-0684.2012.00560.x. Epub 2012 Aug 21. PubMed PMID: 22905903; PubMed Central PMCID: PMC3492523.
    • Mitra R, Baviskar P, Duncan-Decocq RR, Patel D, Oomens AG. The human respiratory syncytial virus matrix protein is required for maturation of viral filaments. J Virol. 2012 Apr;86(8):4432-43. Epub 2012 Feb 8. PubMed PMID:22318136; PubMed Central PMCID: PMC3318654.
    • Peters JL, Demars PL, Collins LM, Stoner JA, Matsumoto H, Komori N, Singh A, Feasley CL, Haddock JA, Levine M. Effects of immunization with natural and recombinant lysine decarboxylase on canine gingivitis development. Vaccine. 2012 Oct 19;30(47):6706-12. doi: 10.1016/j.vaccine.2012.08.028. Epub 2012 Sep 11. PubMed PMID: 22975025.
    • Ponnusamy D, Clinkenbeard KD. Yersinia pestis intracellular parasitism of macrophages from hosts exhibiting high and low severity of plague. PLoS One. 2012;7(7):e42211. Epub 2012 Jul 27. PubMed PMID: 22848745; PubMed Central PMCID: PMC3407133. Shaw JH, Lloyd PG. Post-transcriptional regulation of placenta growth factor mRNA by hydrogen peroxide. Microvasc Res. 2012 Sep;84(2):155-60. Epub 2012 Jun 5. PubMed PMID: 22683469; PubMed Central PMCID: PMC3401273.
    • Simpson KM, Streeter RN, Cramer S, Lamm CG, Love BC. Caudal vena caval thrombosis following treatment of deep digital sepsis. Can Vet J. 2012 Feb;53(2):182-6. PubMed PMID: 22851781; PubMed Central PMCID: PMC3258833.
    • Starkey LA, Panciera RJ, Paras K, Allen KE, Reiskind M, Reichard MV, Johnson EM, Little S. Diversity of Hepatozoon spp. in Coyotes from the Southcentral United States. J Parasitol. 2012 Aug 27. [Epub ahead of print] PubMed PMID: 22924920.


    Progress 10/01/10 to 09/30/11

    Outputs
    OUTPUTS: This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these nonUSDA projects came from Federal, State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

    Impacts
    This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these nonUSDA projects came from Federal, State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Publications

    • Wendte JM, Ponnusamy D, Reiber D, Blair JL, Clinkenbeard KD. In vitro efficacy of antibiotics commonly used to treat human plague against intracellular Yersinia pestis. Antimicrob Agents Chemother. 2011 Aug;55(8):3752-7. Epub 2011 May 31. PubMed PMID: 21628541; PubMed Central PMCID: PMC3147644.
    • Zhou T, Chen Y, Huang C, Chen G. Caffeine induction of sulfotransferases in rat liver and intestine. J Appl Toxicol. 2011 Jul 1. doi: 10.1002/jat.1698. [Epub ahead of print] PubMed PMID: 21721019; PubMed Central PMCID: PMC3189438.
    • Publications: Allen KE, Johnson EM, Little SE. Hepatozoon spp Infections in the United States. Vet Clin North Am Small Anim Pract. 2011 Nov;41(6):1221-38. PubMed PMID: 22041213.
    • Allen KE, Yabsley MJ, Johnson EM, Reichard MV, Panciera RJ, Ewing SA, Little SE. Novel Hepatozoon in vertebrates from the southern United States. J Parasitol. 2011 Aug;97(4):648-53. Epub 2011 Feb 25. PubMed PMID: 21506825.
    • Busby AT, Ayllon N, Kocan KM, Blouin EF, DE LA Fuente G, Galindo RC, Villar M, DE LA Fuente J. Expression of heat shock proteins and subolesin affects stress responses, Anaplasma phagocytophilum infection and questing behaviour in the tick, Ixodes scapularis. Med Vet Entomol. 2011 Jul 22. doi:10.1111/j.1365-2915.2011.00973.x. [Epub ahead of print] PubMed PMID: 21781141.
    • Chen Y, Chen X, Zhang S, Chen G. Influenza A virus infection activates cholesterol sulfotransferase (SULT2B1b) in the lung of female C57BL/6 mice. Biol Chem. 2011 Oct;392(10):869-76. Epub 2011 Aug 28. PubMed PMID: 21871008.
    • Huang C, Zhou T, Chen Y, Sun T, Zhang S, Chen G. Estrogen-related receptor ERRα-mediated down regulation of human hydroxysteroid sulfotransferase (SULT2A1) in Hep G2 cells. Chem Biol Interact. 2011 Jul 15;192(3):264-71. Epub 2011 Apr 13. PubMed PMID: 21513704; PubMed Central PMCID: PMC3111048. Mani RJ, Dye RG, Snider TA, Wang S, Clinkenbeard KD. Bi-cell surface plasmon resonance detection of aptamer mediated thrombin capture in serum. Biosens Bioelectron. 2011 Aug 15;26(12):4832-6. Epub 2011 Jun 6. PubMed PMID: 21700444.
    • McFarlane D, Paradis MR, Zimmel D, Sykes B, Brorsen BW, Sanchez A, Vainio K. The effect of geographic location, breed, and pituitary dysfunction on seasonal adrenocorticotropin and α-melanocyte-stimulating hormone plasma concentrations in horses. J Vet Intern Med. 2011 Jul-Aug;25(4):872-81. doi: 10.1111/j.1939-1676.2011.0745.x. Epub 2011 Jul 11. PubMed PMID: 21745243.
    • McFarlane D, Wolf RF, McDaniel KA, White GL. Age-associated alteration in innate immune response in captive baboons. J Gerontol A Biol Sci Med Sci. 2011 Dec;66(12):1309-17. Epub 2011 Aug 22. PubMed PMID: 21860017; PubMed Central PMCID: PMC3210960.
    • Mishra A, Chintagari NR, Guo Y, Weng T, Su L, Liu L. Purinergic P2X7 receptor regulates lung surfactant secretion in a paracrine manner. J Cell Sci. 2011 Feb 15;124(Pt 4):657-68. Epub 2011 Jan 25. PubMed PMID: 21266468; PubMed Central PMCID: PMC3031375.
    • Ponnusamy D, Hartson SD, Clinkenbeard KD. Intracellular Yersinia pestis expresses general stress response and tellurite resistance proteins in mouse macrophages. Vet Microbiol. 2011 May 12;150(1-2):146-51. Epub 2011 Jan 11. PubMed PMID: 21295415.
    • Reichard MV, Gray KM, Van den Bussche RA, d'Offay JM, White GL, Simecka CM, Wolf RF. Detection and experimental transmission of a novel Babesia isolate in captive olive baboons (Papio cynocephalus anubis). J Am Assoc Lab Anim Sci. 2011 Jul;50(4):500-6. PubMed PMID: 21838979; PubMed Central PMCID: PMC3148650.
    • Reichard MV, Tiernan KE, Paras KL, Interisano M, Reiskind MH, Panciera RJ, Pozio E. Detection of Trichinella murrelli in coyotes (Canis latrans) from Oklahoma and North Texas. Vet Parasitol. 2011 Dec 15;182(2-4):368-71. Epub 2011 Jun 13. PubMed PMID: 21723041.
    • Shaw JH, Xiang L, Shah A, Yin W, Lloyd PG. Placenta growth factor expression is regulated by hydrogen peroxide in vascular smooth muscle cells. Am J Physiol Cell Physiol. 2011 Feb;300(2):C349-55. Epub 2010 Dec 1. PubMed PMID: 21123739; PubMed Central PMCID: PMC3043626.
    • Skaggs JS, Holyoak GR, Montelongo M, Ayalew S, Confer AW. Serum antibody responses in horses and mice following immunization with Actinobacillus equuli outer membrane proteins and recombinant Aqx toxin. Vet J. 2011 Nov;190(2):e91-4. Epub 2011 May 18. PubMed PMID: 21596597.
    • Snider TA, Sepoy C, Holyoak GR. Equine endometrial biopsy reviewed: observation, interpretation, and application of histopathologic data. Theriogenology. 2011 Jun;75(9):1567-81. Epub 2011 Feb 26. Review. PubMed PMID: 21356552.
    • Tyapochkin E, Kumar VP, Cook PF, Chen G. Reaction product affinity regulates activation of human sulfotransferase 1A1 PAP sulfation. Arch Biochem Biophys. 2011 Feb 15;506(2):137-41. Epub 2010 Nov 25. PubMed PMID: 21111704; PubMed Central PMCID: PMC3049928.


    Progress 10/01/09 to 09/30/10

    Outputs
    OUTPUTS: This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects came from Federal, State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

    Impacts
    The interdisciplinary research activity in the CVHS centered on focus areas in infectious disease, parasitology, health and performance, and animal models of human disease, provides an underlying infrastructure for basic research into veterinary science and medicine. This infrastructure adds value to our service and teaching missions, and provides a critical resource for Oklahoma in animal and public health.

    Publications

    • Galindo RC, Ayllon N, Carta T, Vicente J, Kocan KM, Gortazar C, de la Fuente J. Characterization of pathogen-specific expression of host immune response genes in Anaplasma and Mycobacterium species infected ruminants. Comp Immunol Microbiol Infect Dis. 2010 Dec;33(6):e133-42. Epub 2010 Oct 16. PubMed PMID: 20952064.
    • Jiang Z, Piao D, Holyoak GR, Ritchey JW, Bartels KE, Slobodov G, Bunting CF, Krasinski JS. Trans-rectal Ultrasound-coupled Spectral Optical Tomography of Total Hemoglobin Concentration Enhances Assessment of the Laterality and Progression of a Transmissible Venereal Tumor in Canine Prostate. Urology. 2010 Sep 6. [Epub ahead of print] PubMed PMID: 20822801.
    • Jaworski DC, Zou Z, Bowen CJ, Wasala NB, Madden R, Wang Y, Kocan KM, Jiang H, Dillwith JW. Pyrosequencing and characterization of immune response genes from the American dog tick, Dermacentor variabilis (L.). Insect Mol Biol. 2010 Oct;19(5):617-30. doi: 10.1111/j.1365-2583.2010.01037.x. Epub 2010 Aug 5. PubMed PMID: 20698900.
    • Singh K, Ritchey JW, Confer AW. Mannheimia haemolytica: Bacterial-Host Interactions in Bovine Pneumonia. Vet Pathol. 2010 Aug 4. [Epub ahead of print] PubMed PMID: 20685916. Panciera RJ, Confer AW. Pathogenesis and pathology of bovine pneumonia. Vet Clin North Am Food Anim Pract. 2010 Jul;26(2):191-214. Review. PubMed PMID: 20619179.
    • Singh K, Confer AW, Hope JC, Rizzi T, Wyckoff JH 3rd, Weng HY, Ritchey JW. Cytotoxicity and cytokine production by bovine alveolar macrophages challenged with wild type and leukotoxin-deficient Mannheimia haemolytica. Vet J. 2010 Jun 11. [Epub ahead of print] PubMed PMID: 20542456.
    • Taylor JD, Fulton RW, Dabo SM, Lehenbauer TW, Confer AW. Comparison of genotypic and phenotypic characterization methods for Pasteurella multocida isolates from fatal cases of bovine respiratory disease. J Vet Diagn Invest. 2010 May;22(3):366-75. PubMed PMID: 20453209.
    • Snider TA, Confer AW, Payton ME. Pulmonary histopathology of Cytauxzoon felis infections in the cat. Vet Pathol. 2010 Jul;47(4):698-702. Epub 2010 May 4. PubMed PMID: 20442419.
    • Torina A, Galindo RC, Vicente J, Di Marco V, Russo M, Aronica V, Fiasconaro M, Scimeca S, Alongi A, Caracappa S, Kocan KM, Gortazar C, de la Fuente J. Characterization of Anaplasma phagocytophilum and A. ovis infection in a naturally infected sheep flock with poor health condition. Trop Anim Health Prod. 2010 Oct;42(7):1327-31. Epub 2010 Apr 20. PubMed PMID: 20405320.
    • Walz PH, Grooms DL, Passler T, Ridpath JF, Tremblay R, Step DL, Callan RJ, Givens MD; American College of Veterinary Internal Medicine. Control of bovine viral diarrhea virus in ruminants. J Vet Intern Med. 2010 May-Jun;24(3):476-86. Epub 2010 Apr 6. PubMed PMID: 20384958.
    • Ayalew S, Confer AW, Hartson SD, Shrestha B. Immunoproteomic analyses of outer membrane proteins of Mannheimia haemolytica and identification of potential vaccine candidates. Proteomics. 2010 Jun;10(11):2151-64. PubMed PMID: 20336679.
    • Zivkovic Z, Esteves E, Almazan C, Daffre S, Nijhof AM, Kocan KM, Jongejan F, de la Fuente J. Differential expression of genes in salivary glands of male Rhipicephalus (Boophilus)microplus in response to infection with Anaplasma marginale. BMC Genomics. 2010 Mar 18;11:186. PubMed PMID: 20298599; PubMed Central PMCID: PMC2848250.
    • Ridpath JF, Fulton RW, Kirkland PD, Neill JD. Prevalence and antigenic differences observed between Bovine viral diarrhea virus subgenotypes isolated from cattle in Australia and feedlots in the southwestern United States. J Vet Diagn Invest. 2010 Mar;22(2):184-91. PubMed PMID: 20224075.
    • Holland BP, Burciaga-Robles LO, VanOverbeke DL, Shook JN, Step DL, Richards CJ, Krehbiel CR. Effect of bovine respiratory disease during preconditioning on subsequent feedlot performance, carcass characteristics, and beef attributes. J Anim Sci. 2010 Jul;88(7):2486-99. Epub 2010 Feb 26. PubMed PMID: 20190167.
    • Zivkovic Z, Torina A, Mitra R, Alongi A, Scimeca S, Kocan KM, Galindo RC, Almazan C, Blouin EF, Villar M, Nijhof AM, Mani R, La Barbera G, Caracappa S, Jongejan F, de la Fuente J. Subolesin expression in response to pathogen infection in ticks. BMC Immunol. 2010 Feb 19;11:7. PubMed PMID: 20170494; PubMed Central PMCID: PMC2836984.
    • Burciaga-Robles LO, Step DL, Krehbiel CR, Holland BP, Richards CJ, Montelongo MA, Confer AW, Fulton RW. Effects of exposure to calves persistently infected with bovine viral diarrhea virus type 1b and subsequent infection with Mannheima haemolytica on clinical signs and immune variables: model for bovine respiratory disease via viral and bacterial interaction. J Anim Sci. 2010 Jun;88(6):2166-78. Epub 2010 Feb 12. PubMed PMID: 20154164.
    • Burciaga-Robles LO, Krehbiel CR, Step DL, Holland BP, Richards CJ, Montelongo MA, Confer AW, Fulton RW. Effects of exposure to calves persistently infected with bovine viral diarrhea virus type 1b and Mannheimia haemolytica challenge on animal performance, nitrogen balance, and visceral organ mass in beef steers. J Anim Sci. 2010 Jun;88(6):2179-88. Epub 2010 Feb 12. PubMed PMID: 20154160.
    • Fulton RW, Whitley EM, Johnson BJ, Ridpath JF, Kapil S, Burge LJ, Cook BJ, Confer AW. Prevalence of bovine viral diarrhea virus (BVDV) in persistently infected cattle and BVDV subtypes in affected cattle in beef herds in south central United States. Can J Vet Res. 2009 Oct;73(4):283-91. PubMed PMID: 20046630; PubMed Central PMCID: PMC2757709.
    • Williamson KK, Willard MD, Payton ME, Davis MS. Efficacy of omeprazole versus high-dose famotidine for prevention of exercise-induced gastritis in racing Alaskan sled dogs. J Vet Intern Med. 2010 Mar-Apr;24(2):285-8. PubMed PMID: 20102495.


    Progress 10/01/08 to 09/30/09

    Outputs
    OUTPUTS: This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $xxxxxxxx in the period covered by this report. Part of those funds came from Federal sources; the balance came from State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

    Impacts
    The interdisciplinary research activity in the CVHS centered on focus areas in infectious disease, parasitology, health and performance, and animal models of human disease, provides an underlying infrastructure for basic research into veterinary science and medicine. This infrastructure adds value to our service and teaching missions, and provides a critical resource for Oklahoma in animal and public health.

    Publications

    • Ayalew S, Step DL, Montelongo M, Confer AW. Intranasal vaccination of calves with Mannheimia haemolytica chimeric protein containing the major surface epitope of outer membrane lipoprotein PlpE, the neutralizing epitope of leukotoxin, and cholera toxin subunit B. Vet Immunol Immunopathol. 2009 Jun 17. [Epub ahead of print] PubMed PMID: 19581005.
    • Blouin EF, Manzano-Roman R, de la Fuente J, Kocan KM. Defining the role of subolesin in tick cell culture by use of RNA interference. Ann N Y Acad Sci. 2008 Dec;1149:41-4. PubMed PMID: 19120170.
    • Breshears MA, Kocan KM, Johnson EM, Panciera RJ. Light and transmission electron microscopic characteristics of a novel Hepatozoon spp. in naturally infected cotton rats (Sigmodon hispidus). Parasitol Res. 2009 Oct;105(5):1327-32. Epub 2009 Jul 21. PubMed PMID: 19629526.
    • Broaddus CC, Lamm CG, Kapil S, Dawson L, Holyoak GR. Bovine viral diarrhea virus abortion in goats housed with persistently infected cattle. Vet Pathol. 2009 Jan;46(1):45-53. PubMed PMID: 19112114.
    • Burciaga-Robles LO, Holland BP, Step DL, Krehbiel CR, McMillen GL, Richards CJ, Sims LE, Jeffers JD, Namjou K, McCann PJ. Evaluation of breath biomarkers and serum haptoglobin concentration for diagnosis of bovine respiratory disease in heifers newly arrived at a feedlot. Am J Vet Res. 2009 Oct;70(10):1291-8. PubMed PMID: 19795945.
    • Canales M, Naranjo V, Almazan C, Molina R, Tsuruta SA, Szabo MP, Manzano-Roman R, Perez de la Lastra JM, Kocan KM, Jimenez MI, Lucientes J, Villar M, de la Fuente J. Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin. Parasitol Res. 2009 Jul;105(1):97-111. Epub 2009 Feb 21. PubMed PMID: 19229557.
    • Coetzee JF, Kocan KM, Higgins JJ, Apley MD, Jones DE. Ultrastructural and fluorochromatic changes of Anaplasma marginale exposed to oxytetracycline, imidocarb and enrofloxacin in short-term erythrocyte cultures. Vet Microbiol. 2009 Apr 14;136(1-2):45-53. Epub 2008 Oct 25. PubMed PMID: 19054634.
    • de la Fuente J, Blouin EF, Manzano-Roman R, Naranjo V, Almazan C, Perez de la Lastra JM, Zivkovic Z, Massung RF, Jongejan F, Kocan KM. Differential expression of the tick protective antigen subolesin in anaplasma marginale- and A. phagocytophilum-infected host cells. Ann N Y Acad Sci. 2008 Dec;1149:27-35. PubMed PMID: 19120168.
    • Estrada-Pena A, Naranjo V, Acevedo-Whitehouse K, Mangold AJ, Kocan KM, de la Fuente J. Phylogeographic analysis reveals association of tick-borne pathogen, Anaplasma marginale, MSP1a sequences with ecological traits affecting tick vector performance. BMC Biol. 2009 Sep 1;7:57. PubMed PMID: 19723295; PubMed Central PMCID: PMC2741432.
    • Esteves E, Bastos CV, Zivkovic Z, de La Fuente J, Kocan K, Blouin E, Ribeiro MF, Passos LM, Daffre S. Propagation of a Brazilian isolate of Anaplasma marginale with appendage in a tick cell line (BME26) derived from Rhipicephalus (Boophilus) microplus. Vet Parasitol. 2009 Apr 6;161(1-2):150-3. Epub 2008 Dec 13. PubMed PMID: 19150177.
    • Fulton RW, Hessman BE, Ridpath JF, Johnson BJ, Burge LJ, Kapil S, Braziel B, Kautz K, Reck A. Multiple diagnostic tests to identify cattle with Bovine viral diarrhea virus and duration of positive test results in persistently infected cattle. Can J Vet Res. 2009 Apr;73(2):117-24. PubMed PMID: 19436580; PubMed Central PMCID: PMC2666316.
    • Galindo RC, Ayoubi P, Naranjo V, Gortazar C, Kocan KM, de la Fuente J. Gene expression profiles of European wild boar naturally infected with Mycobacterium bovis. Vet Immunol Immunopathol. 2009 May 15;129(1-2):119-25. Epub 2008 Dec 11. PubMed PMID: 19131115.
    • Galindo RC, Doncel-Perez E, Zivkovic Z, Naranjo V, Gortazar C, Mangold AJ, Martin-Hernando MP, Kocan KM, de la Fuente J. Tick subolesin is an ortholog of the akirins described in insects and vertebrates. Dev Comp Immunol. 2009 Apr;33(4):612-7. Epub 2008 Nov 28. PubMed PMID: 19041667.
    • Galindo RC, Munoz PM, de Miguel MJ, Marin CM, Blasco JM, Gortazar C, Kocan KM, de la Fuente J. Characterization of possible correlates of protective response against Brucella ovis infection in rams immunized with the B. melitensis Rev 1 vaccine. Vaccine. 2009 May 18;27(23):3039-44. Epub 2009 Apr 7. PubMed PMID: 19428917.
    • Galindo RC, Munoz PM, de Miguel MJ, Marin CM, Blasco JM, Gortazar C, Kocan KM, de la Fuente J. Differential expression of inflammatory and immune response genes in rams experimentally infected with a rough virulent strain of Brucella ovis. Vet Immunol Immunopathol. 2009 Feb 15;127(3-4):295-303. Epub 2008 Nov 5. PubMed PMID: 19056128.
    • Hessman BE, Fulton RW, Sjeklocha DB, Murphy TA, Ridpath JF, Payton ME. Evaluation of economic effects and the health and performance of the general cattle population after exposure to cattle persistently infected with bovine viral diarrhea virus in a starter feedlot. Am J Vet Res. 2009 Jan;70(1):73-85. PubMed PMID: 19119951.
    • Holland BP, Krehbiel CR, Hilton GG, Streeter MN, Vanoverbeke DL, Shook JN, Step DL, Burciaga-Robles LO, Stein DR, Yates DA, Hutcheson JP, Nichols WT, Montgomery JL. Effect of extended withdrawal of zilpaterol hydrochloride on performance and carcass traits in finishing beef steers. J Anim Sci. 2009 Sep 11. [Epub ahead of print] PubMed PMID: 19749012.
    • Jiang Z, Holyoak GR, Bartels KE, Ritchey JW, Xu G, Bunting CF, Slobodov G, Piao D. In vivo trans-rectal ultrasound-coupled optical tomography of a transmissible venereal tumor model in the canine pelvic canal. J Biomed Opt. 2009 May-Jun;14(3):030506. PubMed PMID: 19566288.
    • Kocan KM, de la Fuente J, Blouin EF, Coetzee JF, Ewing SA. The natural history of Anaplasma marginale. Vet Parasitol. 2009 Sep 19. [Epub ahead of print] PubMed PMID: 19811876.
    • Kocan KM, Zivkovic Z, Blouin EF, Naranjo V, Almazan C, Mitra R, de la Fuente J. Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis. BMC Dev Biol. 2009 Jul 16;9:42. PubMed PMID: 19607704; PubMed Central PMCID: PMC2714835.
    • Lamm CG, Broaddus CC, Holyoak GR. Distribution of bovine viral diarrhea virus antigen in aborted fetal and neonatal goats by immunohistochemistry. Vet Pathol. 2009 Jan;46(1):54-8. PubMed PMID: 19112115.
    • Reichard MV, Meinkoth JH, Edwards AC, Snider TA, Kocan KM, Blouin EF, Little SE. Transmission of Cytauxzoon felis to a domestic cat by Amblyomma americanum. Vet Parasitol. 2009 Apr 6;161(1-2):110-5. Epub 2008 Dec 25. PubMed PMID: 19168288.
    • Ruybal P, Moretta R, Perez A, Petrigh R, Zimmer P, Alcaraz E, Echaide I, Torioni de Echaide S, Kocan KM, de la Fuente J, Farber M. Genetic diversity of Anaplasma marginale in Argentina. Vet Parasitol. 2009 May 26;162(1-2):176-80. Epub 2009 Feb 12. PubMed PMID: 19285808.
    • Smith A, Guo X, de la Fuente J, Naranjo V, Kocan KM, Kaufman WR. The impact of RNA interference of the subolesin and voraxin genes in male Amblyomma hebraeum (Acari: Ixodidae) on female engorgement and oviposition. Exp Appl Acarol. 2009 Jan;47(1):71-86. Epub 2008 Oct 1. PubMed PMID: 18830675.
    • Zivkovic Z, Blouin EF, Manzano-Roman R, Almazan C, Naranjo V, Massung RF, Jongejan F, Kocan KM, de la Fuente J. Anaplasma phagocytophilum and Anaplasma marginale Elicit Different Gene Expression Responses in Cultured Tick Cells. Comp Funct Genomics. 2009:705034. Epub 2009 Jul 15. PubMed PMID: 19636428; PubMed Central PMCID: PMC2712686.
    • Step DL, Krehbiel CR, Burciaga-Robles LO, Holland BP, Fulton RW, Confer AW, Bechtol DT, Brister DL, Hutcheson JP, Newcomb HL. Comparison of single vaccination versus revaccination with a modified-live virus vaccine containing bovine herpesvirus-1, bovine viral diarrhea virus (types 1a and 2a), parainfluenza type 3 virus, and bovine respiratory syncytial virus in the prevention of bovine respiratory disease in cattle. J Am Vet Med Assoc. 2009 Sep 1;235(5):580-7. PubMed PMID: 19719452.
    • Step DL, Litherland NB, Burciaga-Robles LO, Breshears MA, Krehbiel CR, Confer AW, Fulton RW, Morgan GL, Thornsberry RM, Fassig SM. Clinical observations, biochemical data, and postmortem and histopathologic findings in young dairy calves fed zeolite clinoptilolite binder combined with milk replacer. Am J Vet Res. 2008 Dec;69(12):1587-94. PubMed PMID: 19046005.
    • Torina A, Alongi A, Naranjo V, Scimeca S, Nicosia S, Di Marco V, Caracappa S, Kocan KM, de la Fuente J. Characterization of anaplasma infections in Sicily, Italy. Ann N Y Acad Sci. 2008 Dec;1149:90-3. PubMed PMID: 19120181.


    Progress 10/01/07 to 09/30/08

    Outputs
    OUTPUTS: This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU Center for Veterinary Health Sciences (CVHS). We are developing strategic alliances with partner institutions and the private sector and growing the supporting infrastructure. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $7,544,705 in the period covered by this report. Part of those funds came from NIH; the balance came from State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded. PARTICIPANTS: Not relevant to this project. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.

    Impacts
    The interdisciplinary research activity in the CVHS centered on focus areas in infectious disease, parasitology, toxicology, orthopedics, lung biology, health and performance, and animal models of human disease, provides an underlying infrastructure for basic research into veterinary science and medicine. This infrastructure adds value to our service and teaching missions, and provides a critical resource for Oklahoma in animal and public health.

    Publications

    • Step DL, Krehbiel CR, DePra HA, Cranston JJ, Fulton RW, Kirkpatrick JG, Gill DR, Payton ME, Montelongo MA, Confer AW. Effects of commingling beef calves from different sources and weaning protocols during a forty-two-day receiving period on performance and bovine respiratory disease. J Anim Sci. 2008 Nov;86(11):3146-58. Epub 2008 Jun 20. PMID: 18567723
    • Tyapochkin E, Cook PF, Chen G. Isotope exchange at equilibrium indicates a steady state ordered kinetic mechanism for human sulfotransferase. Biochemistry. 2008 Nov 11;47(45):11894-9. Epub 2008 Oct 18. PMID: 18928301
    • Udarbe Zamora EM, Liu J, Pope CN. Effects of chlorpyrifos oxon on M2 muscarinic receptor internalization in different cell types. J Toxicol Environ Health A. 2008;71(21):1440-7. PMID: 18800293
    • Valderas MW, Andi B, Barrow WW, Cook PF. Examination of intrinsic sulfonamide resistance in Bacillus anthracis: a novel assay for dihydropteroate synthase. Biochim Biophys Acta. 2008 May;1780(5):848-53. Epub 2008 Mar 10. PMID: 18342015
    • Valderas MW, Barrow WW. Establishment of a method for evaluating intracellular antibiotic efficacy in Brucella abortus-infected Mono Mac 6 monocytes. J Antimicrob Chemother. 2008 Jan;61(1):128-34. Epub 2007 Nov 16. PMID: 18024491
    • Wang P, Chintagari NR, Narayanaperumal J, Ayalew S, Hartson S, Liu L. Proteomic analysis of lamellar bodies isolated from rat lungs. BMC Cell Biol. 2008 Jun 24;34. PMID: 18577212
    • Allen KE, Li Y, Kaltenboeck B, Johnson EM, Reichard MV, Panciera RJ, Little SE. Diversity of Hepatozoon species in naturally infected dogs in the southern United States. Vet Parasitol. 2008 Jul 4;154(3-4):220-5. Epub 2008 Apr 7. PMID: 18485602
    • Chen Y, Huang C, Zhou T, Chen G. Genistein Induction of Human Sulfotransferases in HepG2 and Caco-2 Cells. Basic Clin Pharmacol Toxicol. 2008 Aug 19. [Epub ahead of print] PMID: 18715236
    • Confer AW, Ayalew S, Montelongo M, Step DL, Wray JH, Hansen RD, Panciera RJ. Immunity of cattle following vaccination with a Mannheimia haemolytica chimeric PlpE-LKT (SAC89) protein. Vaccine. 2008 Sep 29. [Epub ahead of print] PMID: 18834912
    • Dabo SM, Confer A, Montelongo M, York P, Wyckoff JH 3rd. Vaccination with Pasteurella multocida recombinant OmpA induces strong but non-protective and deleterious Th2-type immune response in mice. Vaccine. 2008 Aug 12;26(34):4345-51. Epub 2008 Jul 1. PMID: 18598730
    • Davis MS, Davis WC, Ensign WY, Hinchcliff KW, Holbrook TC, Williamson KK. Effects of training and strenuous exercise on hematologic values and peripheral blood leukocyte subsets in racing sled dogs. J Am Vet Med Assoc. 2008 Mar 15;232(6):873-8. PMID: 18341444
    • McFarlane D, Holbrook TC. Cytokine dysregulation in aged horses and horses with pituitary pars intermedia dysfunction. J Vet Intern Med. 2008 Mar-Apr;22(2):436-42. PMID: 18371032
    • McKenzie EC, Hinchcliff KW, Valberg SJ, Williamson KK, Payton ME, Davis MS. Assessment of alterations in triglyceride and glycogen concentrations in muscle tissue of Alaskan sled dogs during repetitive prolonged exercise. Am J Vet Res. 2008 Aug;69(8):1097-103. PMID: 18672977
    • Karanth S, Holbrook T, MacAllister C, Pope CN. Selective inhibition of butyrylcholinesterase in vivo in horses by the feed-through larvacide Equitrol. Regul Toxicol Pharmacol. 2008 Mar;50(2):200-5. Epub 2007 Nov 28. PMID: 18166255
    • de la Fuente J, Maritz-Olivier C, Naranjo V, Ayoubi P, Nijhof AM, Almazan C, Canales M, Perez de la Lastra JM, Galindo RC, Blouin EF, Gortazar C, Jongejan F, Kocan KM. Evidence of the role of tick subolesin in gene expression. BMC Genomics. 2008 Aug 2;372. PMID: 18673577
    • Genova SG, Streeter RN, Simpson KM, Kapil S. Detection of an antigenic group 2 coronavirus in an adult alpaca with enteritis. Clin Vaccine Immunol. 2008 Oct;15(10):1629-32. Epub 2008 Aug 20. PMID: 18716008
    • Gou D, Mishra A, Weng T, Su L, Chintagari NR, Wang Z, Zhang H, Gao L, Wang P, Stricker HM, Liu L. Annexin A2 interactions with Rab14 in alveolar type II cells. J Biol Chem. 2008 May 9;283(19):13156-64. Epub 2008 Mar 10. PMID: 18332131
    • Holyoak GR, Balasuriya UB, Broaddus CC, Timoney PJ. Equine viral arteritis: current status and prevention. Theriogenology. 2008 Aug;70(3):403-14. Epub 2008 May 27. PMID: 18502495
    • Jin N, Guo Y, Sun P, Bell A, Chintagari NR, Bhaskaran M, Rains K, Baviskar P, Chen Z, Weng T, Liu L. Ionotropic GABA receptor expression in the lung during development. Gene Expr Patterns. 2008 Jul;8(6):397-403. Epub 2008 May 3. PMID: 18539546
    • Johnson EM, Allen KE, Breshears MA, Panciera RJ, Little SE, Ewing SA. Experimental transmission of Hepatozoon americanum to rodents. Vet Parasitol. 2008 Feb 14;151(2-4):164-9. Epub 2007 Nov 4. PMID: 18055118
    • Johnson EM, Allen KE, Panciera RJ, Little SE, Ewing SA. Infectivity of Hepatozoon americanum cystozoites for a dog. Vet Parasitol. 2008 Jun 14;154(1-2):148-50. Epub 2008 Feb 29. PMID: 18387744
    • Kapil S, Lamm CG. Emerging and reemerging viruses of dogs and cats. Preface. Vet Clin North Am Small Anim Pract. 2008 Jul;38(4):xiii-xiv. No abstract available. PMID: 18501275
    • Kirkpatrick JG, Step DL, Payton ME, Richards JB, McTague LF, Saliki JT, Confer AW, Cook BJ, Ingram SH, Wright JC. Effect of age at the time of vaccination on antibody titers and feedlot performance in beef calves. J Am Vet Med Assoc. 2008 Jul 1;233(1):136-42. PMID: 18593324
    • Kocan KM, de la Fuente J, Manzano-Roman R, Naranjo V, Hynes WL, Sonenshine DE. Silencing expression of the defensin, varisin, in male Dermacentor variabilis by RNA interference results in reduced Anaplasma marginale infections. Exp Appl Acarol. 2008 Jun 4. [Epub ahead of print] PMID: 18523848
    • Love BC, Rostagno MH. Comparison of five culture methods for Salmonella isolation from swine fecal samples of known infection status. J Vet Diagn Invest. 2008 Sep;20(5):620-4. PMID: 18776096
    • Manzano-Roman R, Almazan C, Naranjo V, Blouin EF, Kocan KM, de la Fuente J. Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism. J Med Microbiol. 2008 Feb;57(Pt 2):159-63. PMID: 18201980
    • Maxwell LK, Bentz BG, Bourne DW, Erkert RS. Pharmacokinetics of valacyclovir in the adult horse. J Vet Pharmacol Ther. 2008 Aug;31(4):312-20. PMID: 18638291
    • Reichard MV, Baum KA, Cadenhead SC, Snider TA. Temporal occurrence and environmental risk factors associated with cytauxzoonosis in domestic cats. Vet Parasitol. 2008 Apr 15;152(3-4):314-20. Epub 2008 Jan 4. PMID: 18272289
    • Reichard MV, Roman RM, Kocan KM, Blouin EF, Fuente JD, Snider TA, Heinz RE, West MD, Little SE, Massung RF. Inoculation of White-Tailed Deer (Odocoileus Virginianus) with Ap-V1 Or NY-18 Strains of Anaplasma Phagocytophilum and Microscopic Demonstration of Ap-V1 In Ixodes Scapularis Adults that Acquired Infection from Deer as Nymphs. Vector Borne Zoonotic Dis. 2008 Oct 30. [Epub ahead of print] PMID: 18973438
    • Nallapaneni A, Liu J, Karanth S, Pope C. Pharmacological enhancement of endocannabinoid signaling reduces the cholinergic toxicity of diisopropylfluorophosphate. Neurotoxicology. 2008 Aug 13. [Epub ahead of print] PMID: 18765251
    • Posey T, Weng T, Chen Z, Chintagari NR, Wang P, Jin N, Stricker H, Liu L. Arsenic-induced changes in the gene expression of lung epithelial L2 cells: implications in carcinogenesis. BMC Genomics. 2008 Mar 3;115. PMID: 18315880
    • Reichard MV, Torretti L, Garvon JM, Dubey JP. Prevalence of antibodies to Toxoplasma gondii in wolverines from Nunavut, Canada. J Parasitol. 2008 Jun;94(3):764-5. PMID: 18605808
    • Reichard MV, Torretti L, Snider TA, Garvon JM, Marucci G, Pozio E. Trichinella T6 and Trichinella nativa in Wolverines (Gulo gulo) from Nunavut, Canada. Parasitol Res. 2008 Aug;103(3):657-61. Epub 2008 Jun 1. PMID: 18516722
    • Scharf BA, Wan CH, Bluth M, Eberle R, Videan EN, Smith E, Coplan J. Lethargy, ulcers, bronchopneumonia and death in two aged female bonnet macaques presumed to be caused by Cercopithicine herpes virus I. J Med Primatol. 2008 Feb;37 Suppl 60-4. PMID: 18269531
    • Mirajkar N, Pope CN. In vitro sensitivity of cholinesterases and [3H]oxotremorine-M binding in heart and brain of adult and aging rats to organophosphorus anticholinesterases. Biochem Pharmacol. 2008 Oct 15;76(8):1047-58. Epub 2008 Aug 12. PMID: 18761328


    Progress 10/01/06 to 09/30/07

    Outputs
    This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $7,713,705 in the period covered by this report. Part of those funds came from NIH; the balance came from State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    N/A

    Publications

    • d'Offay JM, Eberle R, Sucol Y, Schoelkopf L, White MA, Valentine BD, White GL, Lerche NW. Transmission dynamics of simian T-lymphotropic virus type 1 (STLV1) in a baboon breeding colony: predominance of female-to-female transmission. Comp Med. 2007 Feb;57(1):105-14. PMID: 17348298
    • Duggan VE, Holyoak GR, MacAllister CG, Confer AW. Influence of induction of parturition on the neonatal acute phase response in foals. Theriogenology. 2007 Jan 15;67(2):372-81. Epub 2006 Sep 18. PMID: 16982083
    • Duggan VE, Holyoak GR, MacAllister CG, Cooper SR, Confer AW. Amyloid A in equine colostrum and early milk. Vet Immunol Immunopathol. 2007 Jun 29; [Epub ahead of print] PMID: 17681383
    • Finn MJ, Stiles J, Krohne SG. Visual outcome in a group of dogs with ocular blastomycosis treated with systemic antifungals and systemic corticosteroids. Vet Ophthalmol. 2007 Sep-Oct;10(5):299-303. PMID: 17760709
    • Giles JT 3rd, Rochat MC, Snider TA. Surgical management of a thyroglossal duct cyst in a cat. J Am Vet Med Assoc. 2007 Mar 1;230(5):686-9. PMID: 17331051
    • Barrow EL, Barrow WW, Quenelle DC, Westbrook L, Winchester GA, Staas JK. Efficacy of rifabutin-loaded microspheres for treatment of Mycobacterium avium-infected macrophages and mice. Drug Deliv. 2007 Mar;14(3):119-27. PMID: 17454031
    • Barrow EW, Dreier J, Reinelt S, Bourne PC, Barrow WW. In Vitro Efficacy of New Antifolates against Trimethoprim-Resistant Bacillus anthracis. Antimicrob Agents Chemother. 2007 Dec;51(12):4447-52. Epub 2007 Sep 17. PMID: 17875993
    • Bhaskaran M, Kolliputi N, Wang Y, Gou D, Chintagari NR, Liu L. Trans-differentiation of alveolar epithelial type II cells to type I cells involves autocrine signaling by transforming growth factor beta 1 through the Smad pathway. J Biol Chem. 2007 Feb 9;282(6):3968-76. Epub 2006 Dec 11. PMID: 17158884
    • Bock RA, Soulages JL, Barrow WW. Substrate and inhibitor specificity of Mycobacterium avium dihydrofolate reductase. FEBS J. 2007 Jul;27413):3286-98. Epub 2007 Jun 3. PMID: 17542991
    • Boileau MJ, Washburn KE, Clarke CR, Streeter RN, Maxwell LK. Terbutaline pharmacokinetics in cows: preliminary data. Can J Vet Res. 2007 Jan;71(1):70-3. PMID: 17193884
    • Bordelon JT, Rochat MC. Use of a titanium mesh for cranioplasty following radical rostrotentorial craniectomy to remove an ossifying fibroma in a dog. J Am Vet Med Assoc. 2007 Dec 1;231(11):1692-5. PMID: 18052805
    • Brunker JD, Hoover JP. B-cell lymphoma in a dog with ehrlichiosis (Ehrlichia canis) and systemic histoplasmosis (Histoplasma capsulatum). Can Vet J. 2007 Mar;48(3):292-5. PMID: 17436907
    • Chen X, Zhang J, Baker SM, Chen G. Human constitutive androstane receptor mediated methotrexate induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1). Toxicology. 2007 Mar 7;231(2-3):224-33. Epub 2006 Dec 22. PMID: 17276571
    • Chen Z, Chintagari NR, Guo Y, Bhaskaran M, Chen J, Gao L, Jin N, Weng T, Liu L. Gene expression of rat alveolar type II cells during hyperoxia exposure and early recovery. Free Radic Biol Med. 2007 Aug 15;43(4):628-42. Epub 2007 May 31. PMID: 17640573
    • Cross NL. Effect of pH on the development of acrosomal responsiveness of human sperm. Andrologia. 2007 Apr;39(2):55-9. PMID: 17430424
    • Davis MS, Williams CC, Meinkoth JH, Malayer JR, Royer CM, Williamson KK, McKenzie EC. Influx of neutrophils and persistence of cytokine expression in airways of horses after performing exercise while breathing cold air. Am J Vet Res. 2007 Feb;68(2):185-9. PMID: 17269885
    • de la Fuente J, Blouin EF, Manzano-Roman R, Naranjo V, Almazan C, Perez de la Lastra JM, Zivkovic Z, Jongejan F, Kocan KM. Functional genomic studies of tick cells in response to infection with the cattle pathogen, Anaplasma marginale. Genomics. 2007 Dec;90(6):712-22. Epub 2007 Oct 26. PMID: 17964755
    • de la Fuente J, Manzano-Roman R, Blouin EF, Naranjo V, Kocan KM. Sp110 transcription is induced and required by Anaplasma phagocytophilum for infection of human promyelocytic cells. BMC Infect Dis. 2007 Sep 20;7:110. PMID: 17883869
    • Gilliam LL, Holbrook TC, Dechant JE, Johnson BJ. Postmortem diagnosis of idiopathic hyperammonemia in a horse. Vet Clin Pathol. 2007 Jun;36(2):196-9. PMID: 17523096
    • Gou D, Weng T, Wang Y, Wang Z, Zhang H, Gao L, Chen Z, Wang P, Liu L. A novel approach for the construction of multiple shRNA expression vectors. J Gene Med. 2007 Sep;9(9):751-63. PMID: 17657830
    • Gou D, Zhang H, Baviskar PS, Liu L. Primer extension-based method for the generation of a siRNA/miRNA expression vector. Physiol Genomics. 2007 Nov 14;31(3):554-62. Epub 2007 Sep 5. PMID: 17804605
    • Wang P, Chintagari NR, Gou D, Su L, Liu L. Physical and functional interactions of SNAP-23 with annexin A2. Am J Respir Cell Mol Biol. 2007 Oct;37(4):467-76. Epub 2007 Jun 15. PMID: 17575076
    • Wang Y, Weng T, Gou D, Chen Z, Chintagari NR, Liu L. Identification of rat lung-specific microRNAs by micoRNA microarray: valuable discoveries for the facilitation of lung research. BMC Genomics. 2007 Jan 24;8:29. PMID: 17250765
    • Williamson KK, Willard MD, McKenzie EC, Royer CM, Payton ME, Davis MS. Efficacy of famotidine for the prevention of exercise-induced gastritis in racing Alaskan sled dogs. J Vet Intern Med. 2007 Sep-Oct;21(5):924-7. PMID: 17939544
    • Holyoak GR, Smith CM, Boyette R, Montelongo M, Wray JH, Ayalew S, Duggan VE, Confer AW. Serum antibodies in mares and foals to Actinobacillus equuli whole cells, outer membrane proteins, and Aqx toxin. Vet Immunol Immunopathol. 2007 Aug 15;118(3-4):310-6. Epub 2007 Jun 3. PMID: 17604847
    • Johnson EM, Allen KE, Breshears MA, Panciera RJ, Little SE, Ewing SA. Experimental transmission of Hepatozoon americanum to rodents. Vet Parasitol. 2007 Nov 4; [Epub ahead of print] PMID: 18055118
    • Johnson EM, Allen KE, Panciera RJ, Ewing SA, Little SE, Reichard MV. Field survey of rodents for Hepatozoon infections in an endemic focus of American canine hepatozoonosis. Vet Parasitol. 2007 Nov 30;150(1-2):27-32. Epub 2007 Oct 22. PMID: 17942230
    • Jones ML, Gilmour MA, Streeter RN. Use of grid keratotomy for the treatment of indolent corneal ulcer in a llama. Can Vet J. 2007 Apr;48(4):416-9. PMID: 17494370
    • Karanth S, Liu J, Ray A, Pope C. Comparative in vivo effects of parathion on striatal acetylcholine accumulation in adult and aged rats. Toxicology. 2007 Oct 8;239(3):167-79. Epub 2007 Jul 13. PMID: 17707571
    • Little SE, Hostetler J, Kocan KM. Movement of Rhipicephalus sanguineus adults between co-housed dogs during active feeding. Vet Parasitol. 2007 Nov 30;150(1-2):139-45. Epub 2007 Sep 27. PMID: 17904292
    • Liu J, Gupta RC, Goad JT, Karanth S, Pope C. Modulation of parathion toxicity by glucose feeding: Is nitric oxide involved? Toxicol Appl Pharmacol. 2007 Mar;219(2-3):106-13. Epub 2006 Nov 11. PMID: 17178140
    • Maiti S, Zhang J, Chen G. Redox regulation of human estrogen sulfotransferase (hSULT1E1). Biochem Pharmacol. 2007 May 1;73(9):1474-81. Epub 2006 Dec 28. PMID: 17266938
    • McKenzie EC, Jose-Cunilleras E, Hinchcliff KW, Holbrook TC, Royer C, Payton ME, Williamson K, Nelson S, Willard MD, Davis MS. Serum chemistry alterations in Alaskan sled dogs during five successive days of prolonged endurance exercise. J Am Vet Med Assoc. 2007 May 15;230(10):1486-92. PMID: 17504039
    • Panciera RJ, Boileau MJ, Step DL. Tympany, acidosis, and mural emphysema of the stomach in calves: report of cases and experimental induction. J Vet Diagn Invest. 2007 Jul;19(4):392-5. PMID: 17609349
    • Sheldon J, Hoover JP, Payton ME. Plasma uric acid, creatinine, and urea nitrogen concentrations after whole blood administration via the gastrointestinal tract in domestic pigeons (Columba livia). J Avian Med Surg. 2007 Jun;21(2):130-4. PMID: 18065134
    • Step DL, Engelken T, Romano C, Holland B, Krehbiel C, Johnson JC, Bryson WL, Tucker CM, Robb EJ. Evaluation of three antimicrobial regimens used as metaphylaxis in stocker calves at high risk of developing bovine respiratory disease. Vet Ther. 2007 Summer;8(2):136-47. PMID: 17616948
    • Valderas MW, Barrow WW. Establishment of a method for evaluating intracellular antibiotic efficacy in Brucella abortus-infected Mono Mac 6 monocytes. J Antimicrob Chemother. 2007 Nov 16; [Epub ahead of print] PMID: 18024491
    • Valderas MW, Bourne PC, Barrow WW. Genetic basis for sulfonamide resistance in Bacillus anthracis. Microb Drug Resist. 2007 Spring;13(1):11-20. PMID: 17536929


    Progress 10/01/05 to 09/30/06

    Outputs
    This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $8,044,133 in the period covered by this report. Part of those funds came from NIH; the balance came from State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    (N/A)

    Publications

    • Barrow EW, Suling WJ, Seitz LE, Reynolds RC, Barrow WW. New antifolate inhibitors for Mycobacterium avium. Med Chem. 2006 Sep;2(5):505-10. PMID: 17017990 [PubMed - in process]
    • Bentz BG, Maxwell LK, Erkert RS, Royer CM, Davis MS, MacAllister CG, Clarke CR. Pharmacokinetics of acyclovir after single intravenous and oral administration to adult horses. J Vet Intern Med. 2006 May-Jun;20(3):589-94. PMID: 16734094 [PubMed - indexed for MEDLINE]
    • Chen J, Chen Z, Chintagari NR, Bhaskaran M, Jin N, Narasaraju T, Liu L. Alveolar type I cells protect rat lung epithelium from oxidative injury. J Physiol. 2006 May 1;572(Pt 3):625-38. PMID: 16497717 [PubMed - indexed for MEDLINE]
    • Chen Z, Chen J, Weng T, Jin N, Liu L. Identification of rat lung--prominent genes by a parallel DNA microarray hybridization. BMC Genomics. 2006 Mar 13;7:47. PMID: 16533406 [PubMed - in process]
    • Chintagari NR, Jin N, Wang P, Narasaraju TA, Chen J, Liu L. Effect of cholesterol depletion on exocytosis of alveolar type II cells. Am J Respir Cell Mol Biol. 2006 Jun;34(6):677-87. Epub 2006 Jan 26. PMID: 16439800 [PubMed - indexed for MEDLINE]
    • Baireddy P, Mirajkar N, Nallapaneni A, Singleton N, Pope CN. Effects of combined, multiple stressors on pyridostigmine-induced acute toxicity in rats. Arch Toxicol. 2006 Aug 30; [Epub ahead of print] PMID: 16944100 [PubMed - as supplied by publisher]
    • Baireddy P, Mirajkar N, Nallapaneni A, Singleton N, Pope CN. Effects of combined, multiple stressors on pyridostigmine-induced acute toxicity in rats. Arch Toxicol. 2006 Aug 30; [Epub ahead of print] PMID: 16944100 [PubMed - as supplied by publisher]
    • de la Fuente J, Atkinson MW, Hogg JT, Miller DS, Naranjo V, Almazan C, Anderson N, Kocan KM. Genetic characterization of Anaplasma ovis strains from bighorn sheep in Montana. J Wildl Dis. 2006 Apr;42(2):381-5. PMID: 16870861 [PubMed - indexed for MEDLINE]
    • de la Fuente J, Atkinson MW, Naranjo V, Fernandez de Mera IG, Mangold AJ, Keating KA, Kocan KM. Sequence analysis of the msp4 gene of Anaplasma ovis strains. Vet Microbiol. 2006 Oct 17; [Epub ahead of print] PMID: 17052866 [PubMed - as supplied by publisher]
    • de la Fuente J, Canales M, Kocan KM. The importance of protein glycosylation in development of novel tick vaccine strategies. Parasite Immunol. 2006 Dec;28(12):687-8. No abstract available. PMID: 17096649 [PubMed - in process]
    • de la Fuente J, Ruybal P, Mtshali MS, Naranjo V, Shuqing L, Mangold AJ, Rodriguez SD, Jimenez R, Vicente J, Moretta R, Torina A, Almazan C, Mbati PM, de Echaide ST, Farber M, Rosario-Cruz R, Gortazar C, Kocan KM. Analysis of world strains of Anaplasma marginale using major surface protein 1a repeat sequences. Vet Microbiol. 2006 Nov 1; [Epub ahead of print] PMID: 17084044 [PubMed - as supplied by publisher]
    • de la Fuente J, Torina A, Naranjo V, Nicosia S, Alongi A, La Mantia F, Kocan KM. Molecular characterization of Anaplasma platys strains from dogs in Sicily, Italy. BMC Vet Res. 2006 Jul 26;2:24. PMID: 16872489 [PubMed - in process]
    • Duggan VE, Holyoak GR, Macallister CG, Confer AW. Influence of induction of parturition on the neonatal acute phase response in foals. Theriogenology. 2006 Sep 16; [Epub ahead of print] PMID: 16982083 [PubMed - as supplied by publisher]
    • Karanth S, Liu J, Mirajkar N, Pope C. Effects of acute chlorpyrifos exposure on in vivo acetylcholine accumulation in rat striatum. Toxicol Appl Pharmacol. 2006 Oct 1;216(1):150-6. Epub 2006 Jun 13. PMID: 16777161 [PubMed - in process]
    • Karanth S, Liu J, Mirajkar N, Pope C. Effects of acute chlorpyrifos exposure on in vivo acetylcholine accumulation in rat striatum. Toxicol Appl Pharmacol. 2006 Oct 1;216(1):150-6. Epub 2006 Jun 13. PMID: 16777161 [PubMed - in process]
    • Nallapaneni A, Liu J, Karanth S, Pope C. Modulation of paraoxon toxicity by the cannabinoid receptor agonist WIN 55,212-2. Toxicology. 2006 Oct 3;227(1-2):173-83. Epub 2006 Aug 5. PMID: 16956707 [PubMed - in process]
    • Nallapaneni A, Liu J, Karanth S, Pope C. Modulation of paraoxon toxicity by the cannabinoid receptor agonist WIN 55,212-2. Toxicology. 2006 Oct 3;227(1-2):173-83. Epub 2006 Aug 5. PMID: 16956707 [PubMed - in process]
    • Naranjo V, Ruiz-Fons F, Hofle U, Fernandez DE Mera IG, Villanua D, Almazan C, Torina A, Caracappa S, Kocan KM, Gortazar C, DE LA Fuente J. Molecular epidemiology of human and bovine anaplasmosis in southern europe. Ann N Y Acad Sci. 2006 Oct;1078:95-9. PMID: 17114686 [PubMed - in process]
    • Ritchey JW, Black DH, Rogers KM, Eberle R. In vivo experimentation with simian herpesviruses: assessment of biosafety and molecular contamination. J Am Assoc Lab Anim Sci. 2006 Mar;45(2):7-12. PMID: 16542036 [PubMed - indexed for MEDLINE]
    • Rogers KM, Black DH, Eberle R. Primary mouse dermal fibroblast cell cultures as an in vitro model system for the differential pathogenicity of cross-species herpesvirus papio 2 infections. Arch Virol. 2006 Nov 23; [Epub ahead of print] PMID: 17122896 [PubMed - as supplied by publisher]
    • Rogers KM, Ritchey JW, Payton M, Black DH, Eberle R. Neuropathogenesis of herpesvirus papio 2 in mice parallels infection with Cercopithecine herpesvirus 1 (B virus) in humans. J Gen Virol. 2006 Feb;87(Pt 2):267-76. PMID: 16432011 [PubMed - indexed for MEDLINE]
    • Singh K, Holbrook TC, Gilliam LL, Cruz RJ, Duffy J, Confer AW. Severe pulmonary disease due to multisystemic eosinophilic epitheliotropic disease in a horse. Vet Pathol. 2006 Mar;43(2):189-93. PMID: 16537938 [PubMed - indexed for MEDLINE]
    • Snider TA, Fabich AJ, Washburn KE, Sims WP, Blair JL, Cohen PS, Conway T, Clinkenbeard KD. Evaluation of a model for Escherichia coli O157:H7 colonization in streptomycin-treated adult cattle. Am J Vet Res. 2006 Nov;67(11):1914-20. PMID: 17078755 [PubMed - in process]
    • Symonds KD, MacAllister CG, Erkert RS, Payton ME. Use of force plate analysis to assess the analgesic effects of etodolac in horses with navicular syndrome. Am J Vet Res. 2006 Apr;67(4):557-61. PMID: 16579745 [PubMed - indexed for MEDLINE]
    • Thirumalapura NR, Ramachandran A, Morton RJ, Malayer JR. Bacterial cell microarrays for the detection and characterization of antibodies against surface antigens. J Immunol Methods. 2006 Feb 20;309(1-2):48-54. Epub 2005 Dec 27. PMID: 16423364 [PubMed - indexed for MEDLINE]
    • Wang Y, Stricker HM, Gou D, Liu L. MicroRNA: past and present. Front Biosci. 2007 Jan 1;12:2316-29. PMID: 17127242 [PubMed - in process]
    • Weng T, Chen Z, Jin N, Gao L, Liu L. Gene expression profiling identifies regulatory pathways involved in the late stage of rat fetal lung development. Am J Physiol Lung Cell Mol Physiol. 2006 Nov;291(5):L1027-37. Epub 2006 Jun 23. PMID: 16798779 [PubMed - in process]
    • Gilmour MA, Cardenas MR, Blaik MA, Bahr RJ, McGinnis JF. Evaluation of a comparative pathogenesis between cancer-associated retinopathy in humans and sudden acquired retinal degeneration syndrome in dogs via diagnostic imaging and western blot analysis. Am J Vet Res. 2006 May;67(5):877-81. PMID: 16649924 [PubMed - indexed for MEDLINE]
    • Jin N, He K, Liu L. qPCR-DAMS: a database tool to analyze, manage, and store both relative and absolute quantitative real-time PCR data. Physiol Genomics. 2006 May 16;25(3):525-7. Epub 2006 Feb 28. PMID: 16507784 [PubMed - in process]
    • Jin N, Kolliputi N, Gou D, Weng T, Liu L. A Novel Function of Ionotropic {gamma}-Aminobutyric Acid Receptors Involving Alveolar Fluid Homeostasis. J Biol Chem. 2006 Nov 24;281(47):36012-20. Epub 2006 Sep 26. PMID: 17003036 [PubMed - in process]


    Progress 10/01/04 to 09/30/05

    Outputs
    his project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $8,341,194 in the period covered by this report. Part of those funds came from NIH; the balance came from State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    (N/A)

    Publications

    • de la Fuente J, Massung RF, Wong SJ, Chu FK, Lutz H, Meli M, von Loewenich FD, Grzeszczuk A, Torina A, Caracappa S, Mangold AJ, Naranjo V, Stuen S, Kocan KM. Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains. J Clin Microbiol. 2005 Mar;43(3):1309-17. PMID: 15750101 [PubMed - indexed for MEDLINE]
    • de la Fuente J, Torina A, Caracappa S, Tumino G, Furla R, Almazan C, Kocan KM. Serologic and molecular characterization of Anaplasma species infection in farm animals and ticks from Sicily. Vet Parasitol. 2005 Nov 5;133(4):357-62. PMID: 16043300 [PubMed - in process]
    • de la Fuente J, Torina A, Naranjo V, Caracappa S, Di Marco V, Alongi A, Russo M, Maggio AR, Kocan KM. Infection with Anaplasma phagocytophilum in a seronegative patient in Sicily, Italy: case report. Ann Clin Microbiol Antimicrob. 2005 Oct 3;4:15. PMID: 16202127 [PubMed - in process]
    • Erkert RS, MacAllister CG, Payton ME, Clarke CR. Use of force plate analysis to compare the analgesic effects of intravenous administration of phenylbutazone and flunixin meglumine in horses with navicular syndrome. Am J Vet Res. 2005 Feb;66(2):284-8. PMID: 15757129 [PubMed - indexed for MEDLINE]
    • Erkert RS, MacAllister CG. Use of a eutectic mixture of lidocaine 2.5
    • Garrett JJ, Kocan AA, Reichard MV, Panciera RJ, Bahr RJ, Ewing SA. Experimental Infection of Adult and Juvenile Coyotes with Domestic Dog and Wild Coyote Isolates of Hepatozoon americanum (Apicomplexa: Adeleorina). J Wildl Dis. 2005 Jul;41(3):588-92. PMID: 16244069 [PubMed - in process]
    • Gilmour MA, Clarke CR, Macallister CG, Dedeo JM, Caudell DL, Morton RJ, Pugh M. Ocular penetration of oral doxycycline in the horse. Vet Ophthalmol. 2005 Sep-Oct;8(5):331-5. PMID: 16178844 [PubMed - in process]
    • Heseltine JC, Panciera DL, Troy GC, Monroe WE, Brooks MB, Feldman BF. Effect of levothyroxine administration on hemostatic analytes in Doberman Pinschers with von Willebrand disease. J Vet Intern Med. 2005 Jul-Aug;19(4):523-7. PMID: 16095169 [PubMed - indexed for MEDLINE]
    • Homer LM, Clarke CR, Weingarten AJ. Effect of dietary fat on oral bioavailability of tepoxalin in dogs. J Vet Pharmacol Ther. 2005 Jun;28(3):287-91. PMID: 15953202 [PubMed - indexed for MEDLINE]
    • Hu HH, MacAllister CG, Payton ME, Erkert RS. Evaluation of the analgesic effects of phenylbutazone administered at a high or low dosage in horses with chronic lameness. J Am Vet Med Assoc. 2005 Feb 1;226(3):414-7. PMID: 15702692 [PubMed - indexed for MEDLINE]
    • Jin N, Narasaraju T, Kolliputi N, Chen J, Liu L. Differential expression of GABAA receptor pi subunit in cultured rat alveolar epithelial cells. Cell Tissue Res. 2005 Aug;321(2):173-83. Epub 2005 May 24. PMID: 15912403 [PubMed - in process]
    • Kacham R, Karanth S, Baireddy P, Liu J, Pope C. Interactive toxicity of chlorpyrifos and parathion in neonatal rats: Role of esterases in exposure sequence-dependent toxicity. Toxicol Appl Pharmacol. 2005 Oct 28; [Epub ahead of print] PMID: 16260018 [PubMed - as supplied by publisher]
    • Liu J, Karanth S, Pope C. Dietary modulation of parathion-induced neurotoxicity in adult and juvenile rats. Toxicology. 2005 Jun 1;210(2-3):135-45. PMID: 15840427 [PubMed - indexed for MEDLINE]
    • Maiti S, Chen X, Chen G. All-trans retinoic acid induction of sulfotransferases. Basic Clin Pharmacol Toxicol. 2005 Jan;96(1):44-53. PMID: 15667595 [PubMed - indexed for MEDLINE]
    • Maiti S, Dutta SM, Baker SM, Zhang J, Narasaraju T, Liu L, Chen G. In vivo and in vitro oxidative regulation of rat aryl sulfotransferase IV (AST IV). J Biochem Mol Toxicol. 2005 Mar-Apr;19(2):109-18. PMID: 15849721 [PubMed - indexed for MEDLINE]
    • Maiti S, Grant S, Baker SM, Karanth S, Pope CN, Chen G. Stress regulation of sulfotransferases in male rat liver. Biochem Biophys Res Commun. 2004 Oct 8;323(1):235-41. PMID: 15351727 [PubMed - indexed for MEDLINE]
    • McKenzie E, Holbrook T, Williamson K, Royer C, Valberg S, Hinchcliff K, Jose-Cunilleras E, Nelson S, Willard M, Davis M. Recovery of muscle glycogen concentrations in sled dogs during prolonged exercise. Med Sci Sports Exerc. 2005 Aug;37(8):1307-12. PMID: 16118576 [PubMed - in process]
    • Poly F, Threadgill D, Stintzi A. Genomic diversity in Campylobacter jejuni: identification of C. jejuni 81-176-specific genes. J Clin Microbiol. 2005 May;43(5):2330-8. PMID: 15872262 [PubMed - indexed for MEDLINE]
    • Pope CN, Karanth S, Liu J, Yan B. Comparative carboxylesterase activities in infant and adult liver and their in vitro sensitivity to chlorpyrifos oxon. Regul Toxicol Pharmacol. 2005 Jun;42(1):64-9. Epub 2005 Feb 24. PMID: 15896444 [PubMed - in process]
    • Reichard MV, Kocan AA, Van Den Bussche RA, Barker RW, Wyckoff JH 3rd, Ewing SA. Sequence variation of the ribosomal DNA second internal transcribed spacer region in two spatially-distinct populations of Amblyomma americanum (L.) (Acari: Ixodidae). J Parasitol. 2005 Apr;91(2):260-3. PMID: 15986597 [PubMed - indexed for MEDLINE]
    • Reichard MV, Van Den Bussche RA, Meinkoth JH, Hoover JP, Kocan AA. A new species of Cytauxzoon from Pallas' cats caught in Mongolia and comments on the systematics and taxonomy of piroplasmids. J Parasitol. 2005 Apr;91(2):420-6. PMID: 15986619 [PubMed - indexed for MEDLINE]
    • Ritchey JW, Payton ME, Eberle R. Clinicopathological characterization of monkey B virus (Cercopithecine herpesvirus 1) infection in mice. J Comp Pathol. 2005 Feb-Apr;132(2-3):202-17. PMID: 15737347 [PubMed - indexed for MEDLINE]
    • Rochat MC. Emerging causes of canine lameness. Vet Clin North Am Small Anim Pract. 2005 Sep;35(5):1233-9, vii. Review. PMID: 16129141 [PubMed - indexed for MEDLINE]
    • Almazan C, Blas-Machado U, Kocan KM, Yoshioka JH, Blouin EF, Mangold AJ, de la Fuente J. Characterization of three Ixodes scapularis cDNAs protective against tick infestations. Vaccine. 2005 Aug 15;23(35):4403-16. PMID: 16005748 [PubMed - indexed for MEDLINE]
    • Almazan C, Kocan KM, Blouin EF, de la Fuente J. Vaccination with recombinant tick antigens for the control of Ixodes scapularis adult infestations. Vaccine. 2005 Sep 6; [Epub ahead of print] PMID: 16153760 [PubMed - as supplied by publisher]
    • de la Fuente J, Torina A, Naranjo V, Caracappa S, Vicente J, Mangold AJ, Vicari D, Alongi A, Scimeca S, Kocan KM. Genetic diversity of Anaplasma marginale strains from cattle farms in the province of Palermo, Sicily. J Vet Med B Infect Dis Vet Public Health. 2005 Jun;52(5):226-9. PMID: 16115096 [PubMed - indexed for MEDLINE]
    • Erkert RS, Macallister CG, Campbell G, Payton ME, Shawley R, Clarke CR. Comparison of topical lidocaine/prilocaine anesthetic cream and local infiltration of 2
    • Bhaskaran M, Chen H, Chen Z, Liu L. Hemoglobin is expressed in alveolar epithelial type II cells. Biochem Biophys Res Commun. 2005 Aug 12;333(4):1348-52. PMID: 15979582 [PubMed - indexed for MEDLINE]
    • Rogers KM, Wolf RF, White GL, Eberle R. Experimental infection of baboons (Papio cynocephalus anubis) with apathogenic and neurovirulent subtypes of herpesvirus papio 2. Comp Med. 2005 Oct;55(5):425-30. PMID: 16270898 [PubMed - in process]
    • Schoonover MJ, Jann HW, Blaik MA. Quantitative comparison of three commonly used treatments for navicular syndrome in horses. Am J Vet Res. 2005 Jul;66(7):1247-51. PMID: 16111165 [PubMed - indexed for MEDLINE]
    • Bordelon JT, Reaugh HF, Rochat MC. Traumatic luxations of the appendicular skeleton. Vet Clin North Am Small Anim Pract. 2005 Sep;35(5):1169-94, vi. Review. PMID: 16129138 [PubMed - indexed for MEDLINE]
    • Breshears MA, Eberle R, Ritchey JW. Temporal progression of viral replication and gross and histological lesions in Balb/c mice inoculated epidermally with Saimiriine herpesvirus 1 (SaHV-1). J Comp Pathol. 2005 Aug-Oct;133(2-3):103-13. PMID: 15964589 [PubMed - indexed for MEDLINE]
    • Chen X, Baker SM, Chen G. Methotrexate induction of human sulfotransferases in Hep G2 and Caco-2 cells. J Appl Toxicol. 2005 Sep-Oct;25(5):354-60. PMID: 15986412 [PubMed - indexed for MEDLINE]
    • Chen Z, Liu L. RealSpot: software validating results from DNA microarray data analysis with spot images. Physiol Genomics. 2005 Apr 14;21(2):284-91. Epub 2005 Feb 1. PMID: 15687483 [PubMed - in process]
    • Cross NL. Reorganization of lipid rafts during capacitation of human sperm. Biol Reprod. 2004 Oct;71(4):1367-73. Epub 2004 Jun 23. PMID: 15215196 [PubMed - indexed for MEDLINE]
    • Dabo SM, Confer AW, Hartson SD. Adherence of Pasteurella multocida to fibronectin. Vet Microbiol. 2005 Oct 31;110(3-4):265-75. PMID: 16168584 [PubMed - in process]
    • Davis M, Williamson K, McKenzie E, Royer C, Payton M, Nelson S. Effect of training and rest on respiratory mechanical properties in racing sled dogs. Med Sci Sports Exerc. 2005 Feb;37(2):337-41. PMID: 15692332 [PubMed - in process]
    • Davis MS, Willard MD, Williamson KK, Steiner JM, Williams DA. Sustained strenuous exercise increases intestinal permeability in racing Alaskan sled dogs. J Vet Intern Med. 2005 Jan-Feb;19(1):34-9. PMID: 15715045 [PubMed - indexed for MEDLINE]
    • de la Fuente J, Ayoubi P, Blouin EF, Almazan C, Naranjo V, Kocan KM. Gene expression profiling of human promyelocytic cells in response to infection with Anaplasma phagocytophilum. Cell Microbiol. 2005 Apr;7(4):549-59. PMID: 15760455 [PubMed - indexed for MEDLINE]
    • de la Fuente J, Lew A, Lutz H, Meli ML, Hofmann-Lehmann R, Shkap V, Molad T, Mangold AJ, Almazan C, Naranjo V, Gortazar C, Torina A, Caracappa S, Garcia-Perez AL, Barral M, Oporto B, Ceci L, Carelli G, Blouin EF, Kocan KM. Genetic diversity of anaplasma species major surface proteins and implications for anaplasmosis serodiagnosis and vaccine development. Anim Health Res Rev. 2005 Jun;6(1):75-89. Review. PMID: 16164010 [PubMed - indexed for MEDLINE]
    • Thirumalapura NR, Morton RJ, Ramachandran A, Malayer JR. Lipopolysaccharide microarrays for the detection of antibodies. J Immunol Methods. 2005 Mar;298(1-2):73-81. PMID: 15847798 [PubMed - indexed for MEDLINE]
    • Washburn K, Johnson R, Clarke CR, Anderson K, Lucas M, Bryson W, Robinson J, Dame K, Hubbard V, Callahan K, Robb E. Penetration of ceftiofur into sterile vs. Mannheimia haemolytica-infected tissue chambers in beef calves after subcutaneous administration of ceftiofur crystalline free acid sterile suspension in the ear pinna. J Vet Pharmacol Ther. 2005 Jun;28(3):247-51. PMID: 15953197 [PubMed - indexed for MEDLINE]
    • Weng T, Jin N, Liu L. Differentiation between amplicon polymerization and nonspecific products in SYBR green I real-time polymerase chain reaction. Anal Biochem. 2005 Jul 1;342(1):167-9. No abstract available. PMID: 15958195 [PubMed - indexed for MEDLINE]
    • Wyckoff JH 3rd, Howland JL, Scott CM, Smith RA, Confer AW. Recombinant bovine interleukin 2 enhances immunity and protection induced by Brucella abortus vaccines in cattle. Vet Microbiol. 2005 Nov 30;111(1-2):77-87. Epub 2005 Oct 19. PMID: 16242273 [PubMed - in process]


    Progress 10/01/03 to 09/30/04

    Outputs
    Diseases This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $9,688,264 in the period covered by this report. Part of those funds came from NIH; the balance came from State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    (N/A)

    Publications

    • Singh TK, Abonyo B, Narasaraju TA, Liu L. Reorganization of cytoskeleton during surfactant secretion in lung type II cells: a role of annexin II. Cell Signal. 2004 Jan;16(1):63-70. PMID: 14607276
    • Xu ZQ, Barrow WW, Suling WJ, Westbrook L, Barrow E, Lin YM, Flavin MT. Anti-HIV natural product (+)-calanolide A is active against both drug-susceptible and drug-resistant strains of Mycobacterium tuberculosis. Bioorg Med Chem. 2004 Mar 1;12(5):1199-207. PMID: 14980631
    • Narasaraju TA, Jin N, Narendranath CR, Chen Z, Gou D, Liu L. Protein nitration in rat lungs during hyperoxia exposure: a possible role of myeloperoxidase. Am J Physiol Lung Cell Mol Physiol. 2003 Nov;285(5):L1037-45. Epub 2003 Aug 15. PMID: 12922978
    • Ohsawa K, Black DH, Sato H, Rogers K, Eberle R. Sequence and genetic arrangement of the UL region of the monkey B virus(Cercopithecine herpesvirus 1) genome and comparison with the UL region of other primate herpesviruses. Arch Virol. 2003 May;148(5):989-97. PMID: 12721804
    • Palyada K, Threadgill D, Stintzi A. Iron acquisition and regulation in Campylobacter jejuni. J Bacteriol. 2004 Jul;186(14):4714-29. PMID: 15231804
    • Barker RW, Kocan AA, Ewing SA, Wettemann RP, Payton ME. Occurrence of the Gulf Coast tick (Acari: Ixodidae) on wild and domestic mammals in north-central Oklahoma. J Med Entomol. 2004 Mar;41(2):170-8. PMID: 15061275 Bentz BG, Ealey KA, Morrow J, Claypool PL, Saliki JT. Seroprevalence of antibodies to Sarcocystis neurona in equids residing in Oklahoma. J Vet Diagn Invest. 2003 Nov;15(6):597-600. PMID: 14667028
    • Berry BA, Krehbiel CR, Confer AW, Gill DR, Smith RA, Montelongo M. Effects of dietary energy and starch concentrations for newly received feedlot calves: I. Growth performance and health. J Anim Sci. 2004 Mar;82(3):837-44. PMID: 15032441
    • Blas-Machado U, Saliki JT, Duffy JC, Caseltine SL. Bovine viral diarrhea virus type 2-induced meningoencephalitis in a heifer. Vet Pathol. 2004 Mar;41(2):190-4. PMID: 15017036
    • Blouin EF, Saliki JT, de la Fuente J, Garcia-Garcia JC, Kocan KM. Antibodies to Anaplasma marginale major surface proteins 1a and 1b inhibit infectivity for cultured tick cells. Vet Parasitol. 2003 Feb 13;111(2-3):247-60. PMID: 12531299
    • Blewett EL, Lewis J, Gadsby EL, Neubauer SR, Eberle R. Isolation of cytomegalovirus and foamy virus from the drill monkey (Mandrillus leucophaeus) and prevalence of antibodies to these viruses amongst wild-born and captive-bred individuals. Arch Virol. 2003 Mar;148(3):423-33. PMID: 12607096
    • Garcia-Garcia JC, de la Fuente J, Bell-Eunice G, Blouin EF, Kocan KM. Glycosylation of Anaplasma marginale major surface protein 1a and its putative role in adhesion to tick cells. Infect Immun. 2004 May;72(5):3022-30. PMID: 15102815
    • Garcia-Garcia JC, de la Fuente J, Blouin EF, Johnson TJ, Halbur T, Onet VC, Saliki JT, Kocan KM. Differential expression of the msp1alpha gene of Anaplasma marginale occurs in bovine erythrocytes and tick cells. Vet Microbiol. 2004 Mar 5;98(3-4):261-72. PMID: 15036535
    • Garcia-Garcia JC, de la Fuente J, Kocan KM, Blouin EF, Halbur T, Onet VC, Saliki JT. Mapping of B-cell epitopes in the N-terminal repeated peptides of Anaplasma marginale major surface protein 1a and characterization of the humoral immune response of cattle immunized with recombinant and whole organism antigens. Vet Immunol Immunopathol. 2004 Apr;98(3-4):137-51. PMID: 15010223
    • Gou D, Narasaraju T, Chintagari NR, Jin N, Wang P, Liu L. Gene silencing in alveolar type II cells using cell-specific promoter in vitro and in vivo. Nucleic Acids Res. 2004 Sep 27;32(17):e134. PMID: 15452203
    • Hamilton DH, Turcot I, Stintzi A, Raymond KN. Large cooperativity in the removal of iron from transferrin at physiological temperature and chloride ion concentration. J Biol Inorg Chem. 2004 Oct 29; [Epub ahead of print] PMID: 15517438 [PubMed - as supplied by publisher]
    • Hofmann-Lehmann R, Meli ML, Dreher UM, Gonczi E, Deplazes P, Braun U, Engels M, Schupbach J, Jorger K, Thoma R, Griot C, Stark KD, Willi B, Schmidt J, Kocan KM, Lutz H. Concurrent infections with vector-borne pathogens associated with fatal hemolytic anemia in a cattle herd in Switzerland. J Clin Microbiol. 2004 Aug;42(8):3775-80. PMID: 15297529
    • Ketz-Riley CJ, Galloway DS, Hoover JP, Rochat MC, Bahr RJ, Ritchey JW, Caudell DL. Paresis secondary to an extradural hematoma in a Sumatran tiger (Pantheratigris sumatrae). J Zoo Wildl Med. 2004 Jun;35(2):208-15. PMID: 15305517
    • Ketz-Riley CJ, Reichard MV, Van den Bussche RA, Hoover JP, Meinkoth J, Kocan AA. An intraerythrocytic small piroplasm in wild-caught Pallas's cats (Otocolobusmanul) from Mongolia. J Wildl Dis. 2003 Apr;39(2):424-30. PMID: 12910772
    • Ketz-Riley CJ, Ritchey JW, Hoover JP, Johnson CM, Barrie MT. Immunodeficiency associated with multiple concurrent infections in captive Pallas' cats (Otocolobus manul).J Zoo Wildl Med. 2003 Sep;34(3):239-45. PMID: 14582784
    • Linn LL, Bartels KE, Rochat MC, Payton ME, Moore GE. Lumbosacral stenosis in 29 military working dogs: epidemiologic findings and outcome after surgical intervention (1990-1999). Vet Surg. 2003 Jan-Feb;32(1):21-9. PMID: 12520486
    • de la Fuente J, Blouin EF, Kocan KM. Infection exclusion of the rickettsial pathogen anaplasma marginale in the tickvector Dermacentor variabilis. Clin Diagn Lab Immunol. 2003 Jan;10(1):182-4. PMID: 12522060
    • Chattopadhyay S, Sun P, Wang P, Abonyo B, Cross NL, Liu L. Fusion of lamellar body with plasma membrane is driven by the dual action of annexin II tetramer and arachidonic acid. J Biol Chem. 2003 Oct 10;278(41):39675-83. Epub 2003 Aug 05. PMID: 12902340
    • Chen J, Chen Z, Narasaraju T, Jin N, Liu L. Isolation of highly pure alveolar epithelial type I and type II cells from rat lungs. Lab Invest. 2004 Jun;84(6):727-35. PMID: 15077123
    • Chen Z, Jin N, Narasaraju T, Chen J, McFarland LR, Scott M, Liu L. Identification of two novel markers for alveolar epithelial type I and II cells. Biochem Biophys Res Commun. 2004 Jul 2;319(3):774-80. PMID: 15184050
    • de la Fuente J, Garcia-Garcia JC, Barbet AF, Blouin EF, Kocan KM. Adhesion of outer membrane proteins containing tandem repeats of Anaplasma and Ehrlichia species (Rickettsiales: Anaplasmataceae) to tick cells. Vet Microbiol. 2004 Mar 5;98(3-4):313-22. PMID: 15036540
    • de La Fuente J, Passos LM, Van Den Bussche RA, Ribeiro MF, Facury-Filho EJ,Kocan KM. Genetic diversity and molecular phylogeny of Anaplasma marginale isolates from Minas Gerais, Brazil. Vet Parasitol. 2004 May 26;121(3-4):307-16. PMID: 15135871
    • De La Fuente J, Vicente J, Hofle U, Ruiz-Fons F, Fernandez De Mera IG, VanDen Bussche RA, Kocan KM, Gortazar C. Anaplasma infection in free-ranging Iberian red deer in the region of Castilla-La Mancha, Spain. Vet Microbiol. 2004 Jun 3;100(3-4):163-73. PMID: 15145495
    • d'Offay JM, Floyd JG Jr, Eberle R, Saliki JT, Brock KV, D'Andrea GH,McMillan KL. Use of a polymerase chain reaction assay to detect bovine herpesvirus type 2DNA in skin lesions from cattle suspected to have pseudo-lumpy skin disease .J Am Vet Med Assoc. 2003 May 15;222(10):1404-7, 1366-7. PMID: 12762387
    • Parker WB, Barrow EW, Allan PW, Shaddix SC, Long MC, Barrow WW, Bansal N, Maddry JA. Metabolism of 2-methyladenosine in Mycobacterium tuberculosis. Tuberculosis (Edinb). 2004;84(5):327-36. PMID: 15207808
    • Poly F, Threadgill D, Stintzi A. Identification of Campylobacter jejuni ATCC 43431-specific genes by whole microbial genome comparisons. J Bacteriol. 2004 Jul;186(14):4781-95. PMID: 15231810
    • Ramachandran A, Flinchbaugh J, Ayoubi P, Olah GA, Malayer JR. Target discrimination by surface-immobilized molecular beacons designed to detect Francisella tularensis. Biosens Bioelectron. 2004 Feb 15;19(7):727-36. PMID: 14709391
    • Rogers KM, Ealey KA, Ritchey JW, Black DH, Eberle R. Pathogenicity of different baboon herpesvirus papio 2 isolates is characterized by either extreme neurovirulence or complete apathogenicity. J Virol. 2003 Oct;77(20):10731-9. PMID: 14512523
    • Abonyo BO, Gou D, Wang P, Narasaraju T, Wang Z, Liu L. Syntaxin 2 and SNAP-23 are required for regulated surfactant secretion. Biochemistry. 2004 Mar 30;43(12):3499-506. PMID: 15035620


    Progress 10/01/02 to 09/30/03

    Outputs
    Diseases This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $7,674,896 in the period covered by this report. Part of those funds came from NIH; the balance came from State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded

    Impacts
    (N/A)

    Publications

    • De La Fuente J, Kocan KM. Advances in the identification and characterization of protective antigens for recombinant vaccines against tick infestations. Expert Rev Vaccines. 2003 Aug; 2(4): 583-93.
    • Karanth S, Pope C. In vitro inhibition of blood cholinesterase activities from horse, cow, and rat by tetrachlorvinphos. Int J Toxicol. 2003 Nov-Dec; 22(6): 429-33.
    • Bentz BG, Ealey KA, Morrow J, Claypool PL, Saliki JT. Seroprevalence of antibodies to Sarcocystis neurona in equids residing in Oklahoma. J Vet Diagn Invest. 2003 Nov; 15(6): 597-600.
    • de la Fuente J. The fossil record and the origin of ticks (Acari: Parasitiformes: Ixodida). Exp Appl Acarol. 2003; 29(3-4): 331-44.
    • Gilmour MA. Laser applications for corneal disease. Clin Tech Small Anim Pract. 2003 Aug; 18(3): 199-202.
    • Step DL, Ritchey JW, Drost WT, Bahr RJ. Ameloblastic odontoma in the mandible of a llama. Can Vet J. 2003 Oct; 44(10): 824-7.
    • Shaikh J, Karanth S, Chakraborty D, Pruett S, Pope CN. Effects of daily stress or repeated paraoxon exposures on subacute pyridostigmine toxicity in rats. Arch Toxicol. 2003 Oct; 77(10): 576-83.
    • Wynn VM, Davidson EB, Higbee RG, Ritchey JW, Ridgway TD, Bartels KE, Lucroy MD. In vitro effects of pulsed holmium laser energy on canine uroliths and porcine cadaveric urethra. Lasers Surg Med. 2003; 33(4): 243-6.
    • Jann H, Blaik M, Emerson R, Tomioka M, Stein L, Moll D. Healing characteristics of deep digital flexor tenorrhaphy within the digital sheath of horses. Vet Surg. 2003 Sep-Oct; 32(5): 421-30. Barrow EW, Westbrook L, Bansal N, Suling WJ, Maddry JA, Parker WB, Barrow WW. Antimycobacterial activity of 2-methyl-adenosine. J Antimicrob Chemother. 2003 Nov; 52(5): 801-8.
    • Ewing SA, Panciera RJ. American canine hepatozoonosis. Clin Microbiol Rev. 2003 Oct; 16(4): 688-97.
    • Maiti S, Chen G. Methotrexate is a novel inducer of rat liver and intestinal sulfotransferases. Arch Biochem Biophys. 2003 Oct 15; 418(2): 161-8.
    • Elwick KE, Melendez LD, Higbee RG, Blaik MA, Ritchey JW, Lucroy MD. Neodymium:Yttrium-aluminum-Garnet (Nd:YAG) laser ablation of an obstructive urethral polyp in a dog. J Am Anim Hosp Assoc. 2003 Sep-Oct; 39(5): 506-8.
    • Rogers KM, Ealey KA, Ritchey JW, Black DH, Eberle R. Pathogenicity of different baboon herpesvirus papio 2 isolates is characterized by either extreme neurovirulence or complete apathogenicity. J Virol. 2003 Oct; 77(20): 10731-9.
    • Karanth S, Pope C. Age-related effects of chlorpyrifos and parathion on acetylcholine synthesis in rat striatum. Neurotoxicol Teratol. 2003 Sep-Oct; 25(5): 599-606.
    • Panciera RJ, Washburn KE, Streeter RN, Kirkpatrick JG. A familial peripheral neuropathy and glomerulopathy in Gelbvieh calves. Vet Pathol. 2003 Jan; 40(1): 63-70.
    • Drost WT, Cummings CA, Mathew JS, Panciera RJ, Ko JC. Determination of time of onset and location of early skeletal lesions in young dogs experimentally infected with Hepatozoon americanum using bone scintigraphy. Vet Radiol Ultrasound. 2003 Jan-Feb; 44(1): 86-91.
    • Stintzi A. Gene expression profile of Campylobacter jejuni in response to growth temperature variation. J Bacteriol. 2003 Mar; 185(6): 2009-16.
    • Ridgway TD, Lucroy MD. Phototoxic effects of 635-nm light on canine transitional cell carcinoma cells incubated with 5-aminolevulinic acid. Am J Vet Res. 2003 Feb; 64(2): 131-6.
    • Breshears MA, Black DH, Ritchey JW, Eberle R. Construction and in vivo detection of an enhanced green fluorescent protein-expressing strain of Saimiriine herpesvirus 1 (SaHV-1). Arch Virol. 2003 Feb; 148(2): 311-27.
    • Dabo SM, Confer AW, Quijano-Blas RA. Molecular and immunological characterization of Pasteurella multocida serotype A:3 OmpA: evidence of its role in P. multocida interaction with extracellular matrix molecules. Microb Pathog. 2003 Oct; 35(4): 147-57.
    • Shaikh J, Pope CN. Combined forced running stress and subclinical paraoxon exposure have little effect on pyridostigmine-induced acute toxicity in rats. Toxicology. 2003 Aug 28; 190(3): 221-30.
    • De La Fuente J, Golsteyn Thomas EJ, van den Bussche RA, Hamilton RG, Tanaka EE, Druhan SE, Kocan KM. Characterization of Anaplasma marginale isolated from North American bison. Appl Environ Microbiol. 2003 Aug; 69(8): 5001-5.
    • Ewing SA, Panciera RJ, Mathew JS. Persistence of Hepatozoon americanum (Apicomplexa: Adeleorina) in a naturally infected dog. J Parasitol. 2003 Jun; 89(3): 611-3.
    • Chen G, Chen X. Arginine residues in the active site of human phenol sulfotransferase (SULT1A1). J Biol Chem. 2003 Sep 19; 278(38): 36358-64.
    • Erkert RS, Crowson CL, Moll HD, Bentz BG, Confer AW, Blaik MA. Obstruction of the cecocolic orifice by ileocecocolic intussusception following jejunocecostomy in a horse. J Am Vet Med Assoc. 2003 Jun 15; 222(12): 1743-5, 1707.
    • Chen G, Zhang D, Jing N, Yin S, Falany CN, Radominska-Pandya A. Human gastrointestinal sulfotransferases: identification and distribution. Toxicol Appl Pharmacol. 2003 Mar 15; 187(3): 186-97.
    • d'Offay JM, Floyd JG Jr, Eberle R, Saliki JT, Brock KV, D'Andrea GH, McMillan KL. Use of a polymerase chain reaction assay to detect bovine herpesvirus type 2 DNA in skin lesions from cattle suspected to have pseudo-lumpy skin disease. J Am Vet Med Assoc. 2003 May 15; 222(10): 1404-7, 1366-7.
    • Maiti S, Chen G. Tamoxifen induction of aryl sulfotransferase and hydroxysteroid sulfotransferase in male and female rat liver and intestine. Drug Metab Dispos. 2003 May; 31(5): 637-44.
    • Lucroy MD, Bowles MH, Higbee RG, Blaik MA, Ritchey JW, Ridgway TD. Photodynamic therapy for prostatic carcinoma in a dog. J Vet Intern Med. 2003 Mar-Apr; 17(2): 235-7.


    Progress 10/01/01 to 09/30/02

    Outputs
    This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $7,370,734 in the period covered by this report. Part of those funds came from NIH; the balance came from State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    (N/A)

    Publications

    • Meinkoth JH, Kocan AA, Loud SD, Lorenz MD. Clinical and hematologic effects of experimental infection of dogs with recently identified Babesia gibsoni-like isolates from Oklahoma. J Am Vet Med Assoc. 2002 Jan 15;220(2):185-9.
    • Fulton RW, Cook BJ, Step DL, Confer AW, Saliki JT, Payton ME, Burge LJ, Welsh RD, Blood KS. Evaluation of health status of calves and the impact on feedlot performance: assessment of a retained ownership program for postweaning calves. Can J Vet Res. 2002 Jul;66(3):173-80.
    • Ritchey JW, Ealey KA, Payton ME, Eberle R. Comparative pathology of infections with baboon and African green monkey alpha-herpesviruses in mice. J Comp Pathol. 2002 Aug-Oct;127(2-3):150-61.
    • Ohsawa K, Black DH, Sato H, Eberle R. Sequence and genetic arrangement of the U(S) region of the monkey B virus (cercopithecine herpesvirus 1) genome and comparison with the U(S) regions of other primate herpesviruses. J Virol. 2002 Feb;76(3):1516-20.
    • de la Fuente J, Kocan KM, Garcia-Garcia JC, Blouin EF, Claypool PL, Saliki JT. Vaccination of cattle with Anaplasma marginale derived from tick cell culture and bovine erythrocytes followed by challenge-exposure with infected ticks. Vet Microbiol. 2002 Oct 22;89(2-3):239-251.
    • Blouin EF, Kocan KM, de la Fuente J, Saliki JT. Effect of tetracycline on development of Anaplasma marginale in cultured Ixodes scapularis cells. Vet Parasitol. 2002 Jul 29;107(1-2):115-26.
    • Childs KD, Goad DW, Allan MF, Pomp D, Krehbiel C, Geisert RD, Morgan JB, Malayer JR. Differential expression of NAT1 translational repressor during development of bovine intramuscular adipocytes. Physiol Genomics. 2002 Aug 14;10(2):49-56.
    • Davis MS, Lockard AJ, Marlin DJ, Freed AN. Airway cooling and mucosal injury during cold weather exercise. Equine Vet J Suppl. 2002 Sep;(34):413-6.
    • Carter JN, Meredith GL, Montelongo M, Gill DR, Krehbiel CR, Payton ME, Confer AW. Relationship of vitamin E supplementation and antimicrobial treatment with acute-phase protein responses in cattle affected by naturally acquired respiratory tract disease. Am J Vet Res. 2002 Aug;63(8):1111-7.
    • Gatto NT, Dabo SM, Hancock RE, Confer AW. Characterization of, and immune responses of mice to, the purified OmpA-equivalent outer membrane protein of Pasteurella multocida serotype A:3 (Omp28). Vet Microbiol. 2002 Jul 9;87(3):221-35.
    • Erkert RS, MacAllister CG, Higbee R, Moll HD, Reiners S, Crowson C, Bahr R, Bartels KE. Use of a neodymium:yttrium-aluminum-garnet laser to remove exuberant granulation tissue from the esophagus of a horse. J Am Vet Med Assoc. 2002 Aug 1;221(3):403-7, 368.
    • Duggan VE, MacAllister CG, Davis MS. Xylazine-induced attenuation of dorsal displacement of the soft palate associated with epiglottic dysfunction in a horse. J Am Vet Med Assoc. 2002 Aug 1;221(3):399-401, 368.
    • Ewing SA, Mathew JS, Panciera RJ. Transmission of Hepatozoon americanum Apicomplexa: Adeleorina) by ixodids (Acari: Ixodidae). J Med Entomol. 2002 Jul;39(4):631-4.
    • Panciera RJ, Ritchey JW, Baker JE, DiGregorio M. Trigeminal and polyradiculoneuritis in a dog presenting with masticatory muscle atrophy and Horner's syndrome. Vet Pathol. 2002 Jan;39(1):146-9.


    Progress 10/01/00 to 09/30/01

    Outputs
    This project covers agricultural and biomedical research funded by sources other than USDA that contributes to total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $6,054,586 in the period covered by this report. Part of those funds came from NIH; the balance came from State and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    (N/A)

    Publications

    • Loomis-Huff JE, Eberle R, Lockridge KM, Rhodes G, Barry PA. Immunogenicity of a DNA vaccine against herpes B virus in mice and rhesus macaques. Vaccine. 2001 19:4865-73.
    • Breshears MA, Eberle R, Ritchey JW. Characterization of gross and histological lesions in Balb/c mice experimentally infected with herpesvirus saimiri 1 (HVS1). J Comp Pathol. 2001 125:25-33.
    • Confer AW, Suckow MA, Montelongo M, Dabo SM, Miloscio LJ, Gillespie AJ, Meredith GL. Intranasal vaccination of rabbits with Pasteurella multocida A:3 outer membranes that express iron-regulated proteins. Am J Vet Res. 2001 62:697-703.
    • Panciera RJ, Ewing SA, Confer AW. Ocular histopathology of Ehrlichial infections in the dog. Vet Pathol. 2001 38:43-6.
    • Dabo SM, Confer AW, Lu YS. Single primer polymerase chain reaction fingerprinting for Pasteurella multocida isolates from laboratory rabbits. Am J Vet Res. 2000 61:305-9.
    • Clarke JM, Morton RJ, Clarke CR, Fulton RW, Saliki JT. Development of an ex vivo model to study adherence of Mannheimia haemolytica serovar 1 to mucosal tissues of the respiratory tract of cattle. Am J Vet Res. 2001 62:805-11.
    • Kocan KM, Halbur T, Blouin EF, Onet V, de la Fuente J, Garcia-Garcia JC, Saliki JT. Immunization of cattle with Anaplasma marginale derived from tick cell culture. Vet Parasitol. 2001. 102:151-61.
    • Saliki JT, Lehenbauer TW. Monoclonal antibody-based competitive enzyme-linked immunosorbent assay for detection of morbillivirus antibody in marine mammal sera. J Clin Microbiol. 2001 39:1877-81.
    • Mathew JS, Saliki JT, Ewing SA, Lehenbauer TW, Panciera RJ, Malayer JR, Cummings CA, Kocan AA. An indirect enzyme-linked immunosorbent assay for diagnosis of American canine hepatozoonosis. J Vet Diagn Invest. 2001 13:17-21.
    • Saliki JT, Huchzermeier R, Dubovi EJ. Evaluation of a new sandwich ELISA kit that uses serum for detection of cattle persistently infected with BVD virus. Ann N Y Acad Sci. 2000 916:358-63.
    • Blewett EL, White G, Saliki JT, Eberle R. Isolation and characterization of an endogenous cytomegalovirus (BaCMV) from baboons. Arch Virol. 2001 146:1723-38.
    • Washburn KE, Step DL, Kirkpatrick JG, Saliki JT, Panciera RJ. Bluetongue and persistent bovine viral diarrhea virus infection causing generalized edema in an adult bull. J Vet Intern Med. 2000 14:468-9.
    • Cudd LA, Ownby CL, Clarke CR, Sun Y, Clinkenbeard KD. Effects of Mannheimia haemolytica leukotoxin on apoptosis and oncosis of bovine neutrophils. Am J Vet Res. 2001 62:136-41.


    Progress 10/01/99 to 09/30/00

    Outputs
    This project covers agricultural and biomedical research funded by sources other than USDA that contributes to the total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $5,767,176 in the period covered by this report. Part of the funds came from NIH; the balance came from state and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    (N/A)

    Publications

    • Sun Y, Clinkenbeard KD, Ownby CL, Cudd L, Clarke CR, Highlander SK. Ultrastructural characterization of apoptosis in bovine lymphocytes exposed to Pasteurella haemolytica leukotoxin. Am J Vet Res. 2000 Jan;61(1):51-6.
    • Sun Y, Clinkenbeard KD, Cudd LA, Clarke CR, Clinkenbeard PA. Correlation of Pasteurella haemolytica leukotoxin binding with susceptibility to intoxication of lymphoid cells from various species. Infect Immun. 1999 Dec;67(12):6264-9.
    • Wang Z, Clarke CR, Clinkenbeard KD. Role of phospholipase D in Pasteurella haemolytica leukotoxin-induced increase in phospholipase A(2) activity in bovine neutrophils. Infect Immun. 1999 Aug;67(8):3768-72. Li J, Clinkenbeard KD, Ritchey JW. Bovine CD18 identified as a species specific receptor for Pasteurella haemolytica leukotoxin. Vet Microbiol. 1999 Jun 15;67(2):91-7.
    • Li J, Clinkenbeard KD. Lipopolysaccharide complexes with Pasteurella haemolytica leukotoxin Infect Immun. 1999 Jun;67(6):2920-7.
    • Sun Y, Clinkenbeard KD, Clarke C, Cudd L, Highlander SK, Dabo SM. Pasteurella haemolytica leukotoxin induced apoptosis of bovine lymphocytes involves DNA fragmentation. Vet Microbiol. 1999 Mar 1;65(2):153-66.
    • Fulton RW, d'Offay JM, Saliki JT, Burge LJ, Helman RG, Confer AW, Bolin SR,Ridpath JF. Nested reverse transcriptase-polymerase chain reaction (RT-PCR) for typing ruminant pestiviruses: bovine viral diarrhea viruses and border disease virus. Can J Vet Res. 1999 Oct;63(4):276-81.
    • Liang Y, Hudson LC, Levy JK, Ritchey JW, Tompkins WA, Tompkins MB. T cells over expressing interferon-gamma and interleukin-10 are found in both the thymus and secondary lymphoid tissues of feline immunodeficiency virus-infected cats. J Infect Dis. 2000 Feb;181(2):564-75.
    • Fulton RW, Purdy CW, Confer AW, Saliki JT, Loan RW, Briggs RE, Burge LJ. Bovine viral diarrhea viral infections in feeder calves with respiratory disease: interactions with Pasteurella spp., parainfluenza-3 virus, and bovine respiratory syncytial virus. Can J Vet Res. 2000 Jul;64(3):151-9.
    • Fulton RW, Burge LJ. Bovine viral diarrhea virus types 1 and 2 antibody response in calves receiving modified live virus or inactivated vaccines. Vaccine. 2000 Sep 15;19(2-3):264-74.
    • Fulton RW, Saliki JT, Confer AW, Burge LJ, d'Offay JM, Helman RG, Bolin SR,Ridpath JF, Payton ME. Bovine viral diarrhea virus cytopathic and noncytopathic biotypes and type 1 and 2 genotypes in diagnostic laboratory accessions: clinical and necropsy samples from cattle. J Vet Diagn Invest. 2000 Jan;12(1):33-8.
    • Blewett EL, Black DH, Lerche NW, White G, Eberle R. Simian Foamy Virus Infections in a Baboon Breeding Colony. Virology. 2000 Dec 5;278(1):183-193.
    • Ohsawa K, Lehenbauer TW, Eberle R. Herpesvirus papio 2: alternative antigen for use in monkey B virus diagnostic assays. Lab Anim Sci. 1999 Dec;49(6):605-16.


    Progress 10/01/98 to 09/30/99

    Outputs
    This project covers agricultural and biomedical research funded by sources other than USDA that contributes to the total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress and productivity. Expenditures for these non-USDA projects totaled $3,934,234 in the period covered by this report. Part of the funds came from NIH; the balance came from state and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    (N/A)

    Publications

    • Eberle, R., D.H. Black, T.W. Lehenbauer, and G. White. 1998. Shedding and Transmission of Baboon Herpesvirus papio 2 (HVP2) in a Breeding Colony. Lab Anim Sci 48:23-28.
    • Evans J, Ownby CL. Neutralization of edema, hemorrhage and myonecrosis induced by North American crotalid venoms in simulated first-aid treatments. Toxicon 1999;37(4):633-50
    • Johnson, E.M., S.A. Ewing, R.W. Barker, J.C. Fox, D.W. Crow, and K.M. Kocan. 1998. Experimental transmission of Ehrlichia canis (Rickettsiales:Ehrlichieae) by Dermacentor variabilis (Acari:Ixodidae). Vet. Parasitol. 74(2): 277-288.
    • Kocan, A.A., M. Breshears, C. Cummings, R.J. Panciera, S.A. Ewing, and R. Barker. 1999. Naturally occurring hepatozoonosis in coyotes, Canus latrans, from Oklahoma. J Wildl Dis. 1999 35:86-9.
    • Malayer, J.R. and V.M. Woods. 1998. Development of estrogen receptor expression and hormone response in the uterus of the bovine fetus. Journal of Reproduction and Fertility 112:289-300.
    • Malayer, J.R. and V.M. Woods. 1998. Expression of estrogen receptor and maintenance of hormone-responsive phenotype in bovine fetal uterine cells. Domestic Animal Endocrinology 15:141-154.
    • Mathew, J.S., S.A. Ewing, R.J. Panciera, and J.P. Woods. 1998. Experimental transmission of Hepatozoon americanum Vincent-Johnson et. al., 1997 to dogs by the Gulf Coast tick, Amblyomma maculatum Koch. Vet. Parasitol. 80(1): 1-14.
    • McMurry ST, Lochmiller RL, McBee K, Qualls CW Jr. Indicators of immunotoxicity in populations of cotton rats (Sigmodon hispidus) inhabiting an abandoned oil refinery. Ecotoxicol Environ Saf 1999;42(3):223-35
    • Murray, M.J., F. delPiero, S.C. Jeffrey, M.S. Davis, M.O. Furr, E.J. Dubovi, and J.A. Mayo. 1998. Neonatal Equine Herpesvirus Type 1 Infection on a Thoroughbred Breeding Farm. J. Vet. Internal Med. 12:36-41.
    • Melo PA, Ownby CL. Ability of wedelolactone, heparin, and para-bromophenacyl bromide to antagonize the myotoxic effects of two crotaline venoms and their PLA2 myotoxins. Toxicon 1999;37(1):199-215 Murphy, G.L., S.A. Ewing, L.C. Whitworth, J.C. Fox, and A.A. Kocan. 1998. Prevalence of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma. Vet. Parasitol. 79(4): 325-339.
    • Odell GV, Fenton AW, Ownby CL, Doss MP, Schmidt JO. The role of venom citrate. Toxicon 1999;37(3):407-9
    • Odell GV, Ferry PC, Vick LM, Fenton AW, Decker LS, Cowell RL, Ownby CL, Gutierrez JM. Citrate inhibition of snake venom proteases. Toxicon 1998;36(12):1801-6
    • Ownby CL, Selistre de Araujo HS, White SP, Fletcher JE. Lysine 49 phospholipase A2 proteins. Toxicon 1999;37(3):411-45
    • Panciera, R.J., S.A. Ewing, J.S. Mathew, C.A. Cummings, A.A. Kocan, M.A. Breshears, and J.C. Fox. 1998. Observations on tissue stages of Hepatozoon americanum in 19 naturally infected dogs. Vet. Parasitol. 78(4): 265-276.
    • Propst TL, Lochmiller RL, Qualls CW Jr, McBee K. In situ (mesocosm) assessment of immunotoxicity risks to small mammals inhabiting petrochemical waste sites. Chemosphere 1999;38(5):1049-67
    • Qualls CW Jr, Lubet RA, Lochmiller RL, Elangbam CS, Lish JW, Nims RW. Cytochrome P450 induction in feral Cricetid rodents: a review of field and laboratory investigations. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 1998;121(1-3):55-63
    • Salvini TF, Morini CC, Selistre de Araujo HS, Ownby CL. Long-term regeneration of fast and slow murine skeletal muscles after induced injury by ACL myotoxin isolated from Agkistrodon contortrix laticinctus (broad-banded copperhead) venom. Anat Rec 1999;254(4):521-33
    • Smith, A.L., D. Black, and R. Eberle. 1998. Molecular Evidence for Distinct Genotypes of Monkey B Virus (Herpesvirus simiae) Related to the Host Macaque Species. J Virol 72:9224-9232.
    • Bedford JM, Cross NL. 1999. Capacitation of spermatozoa. Encyclopedia of Reproduction 4: 597-602
    • Cross NL. 1998. Role of cholesterol in sperm capacitation. Biology of Reproduction. 59: 7-11.
    • Cross NL. 1999. Effect of methyl-b-cyclodextrin on the acrosomal responsiveness of human sperm. Molecular Reproduction and Development 53: 92-98.
    • Chandra AM, Campbell GA, Reddy G, Qualls CW Jr. Neurotoxicity of 1,3,5-trinitrobenzene (TNB): immunohistochemical study of cerebrovascular permeability. Vet Pathol 1999;36(3):212-20 Cross NL. 1999. Sphingomyelin modulates capacitation of human sperm in vitro. Molecular Biology of the Cell 10: 360a.


    Progress 10/01/97 to 09/30/98

    Outputs
    This project covers agricultural & biomedical research funded by sources other than USDA which contribute to the total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress/productivity. Expenditures for these non-USDA projects totaled $3,256,704 in the period covered by this report. Part of the funds came from NIH & DOD; the balance came from state and private funding sources, including biological and pharmaceutical corporations. Publications listed below are represent-ative of the studies funded.

    Impacts
    (N/A)

    Publications

    • Chandra, A.M.S., C.W. Qualls, Jr., G.A. Campbell, and G. Reddy. Testicular Effects of 1,3,5-trinitrobenzene II. Immunolocalization of Germ Cells Using Proliferating Cell Nuclear Aantigen (PCNA) as an Endogenous Marker. J Toxicol Environ Hlth 54:379-387, 1997.
    • Cross, N.L. and P. Razy-Faulkner. Control of Human Sperm Intracellular pH by Cholesterol and its Relationship to the Response of the Acrosome to Progesterone. Biol Repro 56: 1169-1174, 1997.
    • Hawkins, E.C., M.G. Davidson, D.J. Meuten, J.B. Rottman, S. Kennedy-Stoskopf, J.K.Levy, J.W. Ritchey, L. Cullins, and M.B. Tompkins. Cytologic Identification of Toxoplasma gondii in Bronchoalveolar Lavage Fluid of Experimentally Infected Cats. J Am Vet Med Assoc 210:648-650, 1997.
    • Mathew, J.S., S.A. Ewing, G.L. Murphy, K.M. Kocan, R.E. Corstvet, and J.C. Fox. Characterization of a New Isolate of Ehrlichia platys (Order Rickettsiales) Using Electron Microscopy and Polymerase Chain Reaction. Vet. Parasitol 68(1-2):1-10, 1997.
    • McCann, J.P., S.C. Loo, D.L. Aalseth, and T. Abribat. Differential Effects of GH Stimulation on Fasting and Prandial Metabolism and Plasma IGFs and IGF-Binding Proteins in Lean and obese Sheep. J Endocrinol 154:329-346, 1997.
    • Ownby, C.L., T.R. Colberg, and S.P. White. Isolation, Characterization and Crystallization of a PLA2 Myotoxin from the Venom of the Prairie Rattlesnake (Crotalus viridis viridis). Toxicon 35:111-124, 1997.
    • Welsh R.D., S.L. Vanhooser, L.B. Dye, and R.W. Nieman. Salmonella Infection in Ratites: Diagnosis, Epidemiology, and Clinical Significance. Vet Med 92(2):193-198, 1997.
    • Woods, J.P., K.E. Bartels, E.L. Stair, and S.A. Schafer. Laser-Induced Shock Wave Lithotripsy of Canine Urocystoliths and Nephroliths. Progr Biomed Optics 2970:227-213, 1997.
    • Black, D. and R. Eberle. Detection and Differentiation of Primate a-Herpesviruses by PCR. J Vet Diagn Invest 9:225-231, 1997.


    Progress 10/01/96 to 09/30/97

    Outputs
    This project covers agricultural & biomedical research funded by sources other than USDA which contribute to the total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress/productivity. Expenditures for these non-USDA projects totaled $2,593,958 in the period covered by this report. Part of the funds came from NIH & DOD; the balance came from state and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    (N/A)

    Publications

    • TB Farver, D Holt, T Lehenbauer & WM Greenley. 1997. Application of Composite Estimation in Studies of Animal Population Production with Two-Stage Repeated Sample Designs. Prev Vet Med 30:109-119.
    • WT Drost, CR Berry, EB Breitschwerdt & MG Davidson. 1997. Thracic Radiographic Findings in Dogs Infected with Rickettsia rickettsii. Vet Radiol Ultrasound 38:260-266.
    • C Taylor-MacAllister, CG MacAllister, D Walker & D Aalseth. 1997. Haematology & Serum Biochemistry Evaluation in Normal Postpartum
    • JE Dawson, CK Warner, SA Ewing, SR Telford, RE Corstvet, R Brennan & JG Olson. 1997. Fingerprinting of Ehrlichia species by repetitive element polymerase chain reaction. Am J Trop Med Hyg 57:109-114.
    • DH Black & R Eberle. 1997. Detection & Differentiation of Primate (-Herpesviruses by PCR. J Vet Diagn Invest 9:225-231.
    • RJ Panciera, NT Gatto, MA Crystal, RG Helman & RW Ely. 1997. Canine Hepatozoonosis in Oklahoma. JAAHA 33:221-225.
    • NL Cross & P Mahasreshti. 1997. Protasome Fraction of Seminal Plasma Prevents Sperm from Becoming Acrosomally Responsive to the Agonist Progesterone. Arch Androl 39:39-44.
    • R Eberle, DH Black, EL Blewett & GL White. 1997. Prevalence of Herpesvirus papio 2 in Baboons & Identification of Immunogenic Viral Polypeptides. Lab Anim Sci 47:256-262.
    • RD Welsh, SL Vanhooser, LB Dye & RW Neiman. 1997. Salmonella Infection in Ratites: Diagnosis, Epidemiology & Clinical Significance. Vet Med (Feb):193-198.


    Progress 10/01/95 to 09/30/96

    Outputs
    This project covers agricultural & biomedical research funded by sources other than USDA which contribute to the total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress/productivity. Expenditures for these non-USDA projects totaled $2,708,100 in the period covered by this report. Part of the funds came from NIH & DOD; the balance came from state and private funding sources, including biological and pharmaceutical corporations. Publications listed below are representative of the studies funded.

    Impacts
    (N/A)

    Publications

    • Andrews,FM, CH MacAllister, CC Jenkins & JT Blackford. 1996. Omeprazole: A Promising Antiulcer Drug in Horses. The Compendium (Equine) Nov:1228-1230.
    • Roder,JD, Y Shen & S Sangiah. 1996. The Effects of Verapamil & Lidocaine on the Duration of Propofol & Xylazine-Ketamine Anesthesia in Broiler Chickens. Vet Human Toxicol 38:181-183.
    • Cross, NL 1996. Effect of Cholesterol & Other Sterols on Sperm Acrosomal Responsiveness. Molec Repro Devel 45:212-217.
    • Mathew,JS, SA Ewing, RW Barker, JC Fox, JE Dawson, CK Warner, GL Murphy & KM Kocan. 1996. Attempted Transmission of Erlichia canis by Rhipicephalus sanguineus After Passage in Cell Culture. AJVR 57:1594-1598.
    • Helman,RG, K Fenton, WC Edwards, RJ Panciera & GE Burrows. 1996. Sudden Death in Calves Due to Taxus Ingestion. Agri-Practice 17:16-18.
    • Roder,JD, S Sangiah, JL Lish & CW Qualls Jr. 1996. The Influence of Ionophores on the Duration of Xylazine-Ketamine Anesthesia & the Hepatic Cytochrome P-450 Content of Broiler Chickens. Vet Human Toxicol 38:353-355.
    • Blewett,EL, D Black & R Eberle. 1996. Characterization of Virus-Specific & Cross-Reactive Monoclonal Antibodies to Herpesvirus simiae (B Virus). J Gen Virol 77:2787-2793.
    • Guglick, CG MacAllister, PJ Ewing & AW Confer. 1996. Thrombosis Resulting in Rectal Perforation in a Horse. JAVMA 209:1125-1127.


    Progress 10/01/94 to 09/30/95

    Outputs
    This project covers agricultural and biomedical research funded by sources otherthan USDA and which contribute to the total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress. Expenditures for these non-USDA research projects totaled $2,675,333 in the period covered by this report. Part of the funds came from NIH & DoD; the balance came from state and private funding sources, including biological and pharmaceutical corporations.

    Impacts
    (N/A)

    Publications


      Progress 10/01/93 to 09/30/94

      Outputs
      This project covers agricultural and biomedical research funded by sources otherthan USDA which contributes to the total research capacity of the OSU College of Veterinary Medicine. Consequently, publications are presented as evidence of progress. Expenditures for these non-USDA research projects totaled $2,404,327 in the period covered by this report. Part of the funds came from NIH & DoD; a significant amount came from research awards from the Oklahoma Center for the Advancement of Science & Technology; the balance came from private funding sources, including biological and pharmaceutical corporations.

      Impacts
      (N/A)

      Publications


        Progress 09/01/88 to 09/30/93

        Outputs
        This project covers funding for research in farm animals that is funded by agencies other than USDA; consequently, publications are presented as evidence of progress. These farm animal health projects 'totaled $1,528,194.70 in the period covered. Part of the funds came from the National Science Foundation; a significant amount came from the research awards from the Oklahoma Center for the Stimulation of Science and Technology, and the balance came from private funding sources, chiefly biological and pharmaceutical houses.

        Impacts
        (N/A)

        Publications

        • PANCIERA, R.J., MOSIER, D.A., and RITCHEY, J.W., 1992. Hairy Vetch (Vicia villosa Roth) poisoning in cattle: update and experimental induction of disease. J Vet Diagn Invest 4:317-325.
        • WYCKOFF, J.H., III, HOWLAND, J.L. and CONFER, A.W., 1992. Comparison of Brucella abortus antigen preparations for in vitro stimulation of immune bovine T-lymphocyte cell lines. Vet Immunol Immunopathol 12:1-20.
        • ALAVI, F.K., MCCANN, J.P., MAUROMOUSTAKIS, A.L., and SANGIAH, S., 1993. Feeding behavior and its responsiveness to nalozone differ in lean and obese sheep. Physiol & Behavior 53:317-323.
        • WYCKOFF, J.H., III, HOWLAND, J.L., and CONFER, A.W., 1993. Comparison of Brucella abortus antigen preparations for in vitro stimulation of immune bovine T-lymphocyte cell lines. Vet Immunol and Immunopathol 36:45-64.
        • RODER, J.D., AKKAYA, R., AMOUZADAH, H.R., SANGIAH, S., BURROWS, G., and QUALLS, C.W., 1993. Effects of hepatic P-450 enzyme inhibitors and inducers on the dura.
        • KOCAN, K.M., EWING, S.A., CLAYPOOL, P.L., MCGUIRE, T.C., HAIR, J.A., and BARRON, S.J., 1993. Development of Anaplasma marginale in salivary glands of male Dermacentor andersoni. AM J Vet Res 54(1):196-112.
        • BLOUIN, E.J., BLANKEMEYER, J.T., KOCAN, K.M., and EWING, S.A. 1993. Effect of 4-bromo-calcium ionophore A23187 on release of Anaplasma marginale from bovine erythrocytes in vitro. Am J Vet Res 54(2):263-269.


        Progress 10/01/91 to 09/30/92

        Outputs
        This project covers funding for research in farm animals that is funded by agencies other than USDA; consequently, publications are presented as evidence of progress. These farm animal health projects totaled $639,935.50 in the period covered. Part of the funds came from the National Science Foundation; a significant amount came from the research awards from the Oklahoma Center for the Stimulation of Science and Technology, and the balance came from private funding sources, chiefly biological and pharmaceutical manufacturing firms.

        Impacts
        (N/A)

        Publications

        • BURGA, D.J. and COLLIER, M.W. 1991. T-plate repair of fracture of the nasal bones in a horse. J. Am. Vet. Med. Assn. 199:909-912.
        • DZATA, G.K., CONFER, A.W. and WYCKOFF III, J.H. 1991. The effects of adjuvants on immune responses in cattle injected with a Brucella abortus soluble antigen. Vet. Microbiol. 29:27-48.
        • MURPHY, G.L. and DALLAS 1991. Analysis of two genes encoding heat-labile toxins and located on a single Ent plasmid from Escherichia coli. Gene 103:37-43.
        • HOLLAND, S.P., FULTON, R.W., SHORT JR, E.C., WYCKOFF III, J. H. and FOX, J.C. 1991. In vitro and in vivo 2', 5'-oligoadenylate synthetase activity induced by recombinant DNA-derived bovine interferon alpha I1 in bovine alveolar macrphage.
        • RODGERS, S.J. and BALDWIN, C.A. 1991. Comparison of a commercial visible reaction disc enzyme immunoassay to a commercial spectrophotometric bead enzyme immunoassay and electron microscopy for the diagnosis of rotavirus in bovines.
        • JANN, H.W., COLLIER, M.A. and MATHES, R.A. 1991. Recent advances in equine tendon repair. Equine Practice 13(10):29-34.


        Progress 10/01/90 to 09/30/91

        Outputs
        This project reports progress, through publications, on livestock research that is funded for short periods of time and would otherwise overburden the CRIS system and fail to capture research which is not funded by USDA. Funding for these projects comes from a variety of sources, including:*Industries interested in research and development of marketable products. *Short-term support from the Oklahoma Center for the Advancement of Science and Technology. *Foundations Seed grants from State of Oklahoma appropriations to the College of Veterinary Medicine. *Start-up funds from State of Oklahoma agencies, principally the Oklahoma Department of Wildlife Management. Although each of these is a relatively small, short-term grant, collectively they amount to considerable research support. For FY 1990-91, they total $1,433,390. The published reports of these projects are listed in field 87.

        Impacts
        (N/A)

        Publications


          Progress 10/01/89 to 09/30/90

          Outputs
          This project reports progress, through publications, on livestock research that is funded for short periods of time and would otherwise overburden the CRIS system and fail to capture research which is not funded by USDA. Funding for these projects comes from a variety of sources, including: 1) industries interested in research and development of marketable products. 2) Short-term support from the Oklahoma Center for the Advancement of Science and Technology. 3) Foundations. 4) Seed grants from State of Oklahoma appropriation to the College of Veterinary Medicine. 5) Start-up funds from State of Oklahoma Agencies, principally the Oklahoma Department of Agruiculture and the Oklahoma Department of Wildlife Management. Although each of these is a relatively small, short-term grant, collectively they amount to considerable research support. For FY 1989-89, they total $677,320.

          Impacts
          (N/A)

          Publications


            Progress 10/01/88 to 09/30/89

            Outputs
            Gossypol toxicity in livestock is not a disease of the past or just a problem with hogs. Cottonseed and cottonseed products are among the most economical sources of protein, are used widely in animal feeds, and cases of gossypol toxicity in young ruminants have been found in the field. Gossypol toxicity in young ruminants has now been produced experimentally. High levels of gossypol (greater than 1800 ppm, equivalent to .18 percent) were known to be toxic to ruminants, but we have shown that even very low levels (less than 400 ppm) are toxic to young calves and lambs. The interactive effects of sex steroids and obesity on glucose metabolism and pancreatic secretion and organ removal of insulin wre determined in multi-catheterized lean and obese sheep by multiplying venoarterial concentration differences by plasma flows. We concluded that excessive insulin secretion, not decreased insulin removal, maintains the basal hyperinsulinemia in moderately obese sheep and that the progesterone-to-estradiol ration has marked and divergent effects on insulin and glucose metabolism in individual tissues of sheep, both in the basal state and during an intravenous glucose tolerance test. A distinct causal relationship could not be determined for eosinophilic myositis in cattle and Sarcocystis cruzi.

            Impacts
            (N/A)

            Publications

            • NO PUBLICATIONS REPORTED THIS PERIOD.