Progress 07/01/10 to 06/30/15
Outputs
Target Audience: NMR spectroscopy is used to investigate properties of organic molecules ranging from small molecules to proteins. It is important in understanding life processes at the molecular level from a diversity of research samples. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? Training on the use and operation of the NMR was provided to students, post-doctoral researchers and staff members. How have the results been disseminated to communities of interest? Results from use of the NMR were presented by the research investigators. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful analytical technique that provides atomic level characterization of the solution structure of small and large molecules. Spectra obtained by NMR spectroscopy give scientists information on the composition of organic chemical compounds. This information can be used in a wide variety of research programs to help in the understanding the chemistry underlying many biological questions. The 400 MHz NMR was used by a number of researchers including:Drs. Adrian Hegeman, Jerry Cohen and Paul Boswell (Horticulture), Dr. Gary Reineccius (Food Scienceand Nutrition), Dr Michael Sadowsky (Soil Science), Drs. Larry Wackett, Romas Kazlauskas, Claudia Schmidt- Dannert and Jack Richman(Biochemistry), Drs. Simo Sarkanen and Dharma Kodali (Bioproducts/Biosystems Engineering) and Dr Steve Heilman (BioTechnology Institute).
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2013
Citation:
Dodge AG, Preiner CS, Wackett LP, (2013) Expanding the cyanuric acid hydrolase protein family to the fungal kingdom. J.Bacteriol 2013 Dec(23):5233-41
- Type:
Journal Articles
Status:
Accepted
Year Published:
2013
Citation:
Aukema KG, Makris TM, Stoian SA, Richman JE, Munck E, Lipscomb JD, Wackett LP, Cyanobacterial Aldehyde Deformylase Oxygenation of Aldehydes Yields n-1 Aldehydes and Alcohols in Addition to Alkanes ACS Catal. 2013,3(10),pp2228-2238
- Type:
Journal Articles
Status:
Accepted
Year Published:
2011
Citation:
D. (T.) Yin, Q. Jing, W. Wafa AlDajani, S. Duncan, U. Tschirner, J. Schilling, R. J. Kazlauskas "Improved pretreatment of lignocellulosic biomass using enzymatically-generated peracetic acid" Bioresour. Technol. (2011), 103.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2012
Citation:
Seffernick JL, Erickson JS, Cameron SM, Cho S, Dodge AG, Richman JE, Sadowsky MJ, Wackett LP. (2012) Defining Sequence Space and Reaction Products within the Cyanuric Acid Hydrolase (AtzD)/Barbiturase Protein Family. J Bacteriol. 194(17):4579-88.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2011
Citation:
Boswell, P. G.; Schellenberg, J. R.; Carr, P. W.; Cohen, J. D.; Hegeman, A. D.; Easy and accurate high-performance liquid chromatography retention prediction with different gradients, flow rates, and instruments by back-calculation of gradient and flow rate profiles. Journal of Chromatography A, (2011), 1218, 6742-6749.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2012
Citation:
Boswell, P. G.; Carr, P. W.; Cohen, J. D.; Hegeman, A. D.; Easy and Accurate Calculation of Programmed Temperature Gas Chromatographic Retention Times by Back-Calculation of Temperature and Hold-up Time Profiles. (2012) Journal of Chromatography A.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2011
Citation:
Cameron SM, Durchschein K, Richman JE, Sadowsky MJ, and Wackett LP, New Family of Biuret Hydrolases Involved in s-Triazine Ring Metabolism, (2011) ACS Catalysis, 1 (9), pp 1075-1082
- Type:
Journal Articles
Status:
Accepted
Year Published:
2011
Citation:
Frias JA, Richman JE, Wackett LP. (2011) Purification and characterization of OleA from Xanthomonas campestris and demonstration of a non-decarboxylative Claisen condensation reaction. J Biol Chem 286(13):10930-8
- Type:
Journal Articles
Status:
Accepted
Year Published:
2010
Citation:
Heilmann SM, Jader LR, Harned LA, Sadowsky MJ, Schendel FJ, Lefebvre PA, von Keitz MG, Valentas KJ. (2010) Hydrothermal carbonization of microalgae II. Fatty acid, char, and algal nutrient products. Applied Energy.
|
Progress 10/01/13 to 09/30/14
Outputs
Target Audience: NMR spectroscopy is used to investigate properties of organic molecules ranging from small molecules to proteins. It is important in understanding life processes at the molecular level from a diversity of research samples. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? Training on the use and operation of the NMR was provided to students, post-doctoral researchers and staff members. How have the results been disseminated to communities of interest? Results from use of the NMR were presented by the research investigators. What do you plan to do during the next reporting period to accomplish the goals? The center will continue to provide NMR service to the research community.
Impacts
What was accomplished under these goals?
Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful analytical technique that provides atomic level characterization of the solution structure of small and large molecules. Spectra obtained by NMR spectroscopy give scientists information on the composition of organic chemical compounds. This information can be used in a wide variety of research programs to help in the understanding the chemistry underlying many biological questions. The 400 MHz NMR was used by a number of researchers including:Drs. Adrian Hegeman, Jerry Cohen and Paul Boswell (Horticulture), Dr. Gary Reineccius (Food Scienceand Nutrition), Dr Michael Sadowsky (Soil Science), Drs. Larry Wackett, Romas Kazlauskas, Claudia Schmidt-Dannert and Jack Richman(Biochemistry), Drs. Simo Sarkanen and Dharma Kodali (Bioproducts/Biosystems Engineering) and Dr Steve Heilman (BioTechnology Institute).
Publications
|
Progress 01/01/13 to 09/30/13
Outputs
Target Audience: NMR spectroscopy is important in understanding life processes at the molecular level with results presented by the research scientists to their research areas. Changes/Problems: No major changes. What opportunities for training and professional development has the project provided? Training on the use and operation of the NMR was provided to students, post-doctoral researchersand staff members. How have the results been disseminated to communities of interest? Results from use of the NMR were published in scientific journals by the various investigators. What do you plan to do during the next reporting period to accomplish the goals? The center will continue to provide NMR service to the research community.
Impacts
What was accomplished under these goals?
Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful analytical technique that provides atomic level characterization of the solution structure of small and large molecules. Spectra obtained by NMR spectroscopy give scientists information on the composition of organic chemical compounds. This information can be used in a wide variety of research programs to help in the understanding the chemistry underlying many biological questions. The 400 MHz NMR was used by a number of researchers including:Drs. Adrian Hegeman, Jerry Cohenand Paul Boswell (Horticulture), Dr. Gary Reineccius (Food Scienceand Nutrition), Dr Michael Sadowsky (Soil Science), Drs. Larry Wackett, Romas Kazlauskas, Claudia Schmidt-Dannertand Jack Richman(Biochemistry), Drs. Simo Sarkanen and Dharma Kodali (Bioproducts/Biosystems Engineering) and Dr Steve Heilman (BioTechnology Institute). Over 1400 samples were analyzed using the NMR.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Dodge AG, Preiner CS, Wackett LP, (2013) Expanding the cyanuric acid hydrolase protein family to the fungal kingdom. J.Bacteriol 2013 Dec(23):5233-41
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Aukema KG, Makris TM, Stoian SA, Richman JE, Munck E, Lipscomb JD, Wackett LP, Cyanobacterial Aldehyde Deformylase Oxygenation of Aldehydes Yields n-1 Aldehydes and Alcohols in Addition to Alkanes ACS Catal. 2013,3(10),pp2228-2238
|
Progress 01/01/12 to 12/31/12
Outputs
OUTPUTS: A Varian 400MHz NMR spectrometer was located in room 170 Gortner Laboratory of Biochemistry and was used to obtain spectra used in the elucidation of the chemical structure of biological molecules. Spectra were acquired on the proton and carbon atoms within the molecules. Over 1200 samples were run for research purposes. Data was given to individual research scientists located primarily on the St Paul campus at the University of Minnesota. PARTICIPANTS: Research scientists on the St Paul campus were the primarily users of the instrumentation. Projects presently using the instrument include research from Drs. Adrian Hegeman and Paul Boswell (Horticulture), Dr. Gary Reineccius (Food Science and Nutrition), Dr Michael Sadowsky (Soil Science), Drs. Larry Wackett, Romas Kazlauskas and Jack Richman(Biochemistry), Drs. Simo Sarkanen and Dharma Kodali (Bioproducts/Biosystems Engineering) and Dr Steve Heilman (BioTechnology Institute). TARGET AUDIENCES: NMR spectroscopy is important in understanding life processes at the molecular level with results presented by the research scientists to their research areas. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful analytical technique that provides atomic level characterization of the solution structure of small and large molecules. Spectra obtained by NMR spectroscopy give scientists information on the composition of organic chemical compounds. This information can be used in a wide variety of research programs to help in the understanding the chemistry underlying many biological questions.
Publications
- Seffernick JL, Erickson JS, Cameron SM, Cho S, Dodge AG, Richman JE, Sadowsky MJ, Wackett LP. (2012) Defining Sequence Space and Reaction Products within the Cyanuric Acid Hydrolase (AtzD)/Barbiturase Protein Family. J Bacteriol. 194(17):4579-88.
- Boswell, P. G.; Schellenberg, J. R.; Carr, P. W.; Cohen, J. D.; Hegeman, A. D.; Easy and accurate high-performance liquid chromatography retention prediction with different gradients, flow rates, and instruments by back-calculation of gradient and flow rate profiles. Journal of Chromatography A, (2011), 1218, 6742-6749.
- Boswell, P. G.; Carr, P. W.; Cohen, J. D.; Hegeman, A. D.; Easy and Accurate Calculation of Programmed Temperature Gas Chromatographic Retention Times by Back-Calculation of Temperature and Hold-up Time Profiles. (2012) In press. Journal of Chromatography A.
- D. (T.) Yin, Q. Jing, W. Wafa AlDajani, S. Duncan, U. Tschirner, J. Schilling, R. J. Kazlauskas "Improved pretreatment of lignocellulosic biomass using enzymatically-generated peracetic acid" Bioresour. Technol. (2011), 103.
|
Progress 01/01/11 to 12/31/11
Outputs
OUTPUTS: A Varian 400MHz NMR spectrometer was located in room 170 Gortner Laboratory of Biochemistry and was used to obtain spectra used in the elucidation of the chemical structure of biological molecules. Spectra were acquired on the proton and carbon atoms within the molecules. Over 1600 samples were run for research purposes. Data was given to individual research scientists located primarily on the St Paul campus at the University of Minnesota. PARTICIPANTS: Research Scientists on the St Paul campus were the primary users on the instrumentation. TARGET AUDIENCES: NMR spectroscopy is important in understanding life processes at the molecular level with results presented by the research scientists to their research areas. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Spectra obtained by NMR spectroscopy give scientists information on the composition of organic chemical compounds. This information can be used in a wide variety of research programs to help in the understanding the chemistry underlying many biological questions.
Publications
- Cameron SM, Durchschein K, Richman JE, Sadowsky MJ, and Wackett LP, New Family of Biuret Hydrolases Involved in s-Triazine Ring Metabolism, (2011) ACS Catalysis, 1 (9), pp 1075-1082
- Frias JA, Richman JE, Wackett LP. (2011) Purification and characterization of OleA from Xanthomonas campestris and demonstration of a non-decarboxylative Claisen condensation reaction. J Biol Chem 286(13):10930-8
|
Progress 01/01/10 to 12/31/10
Outputs
OUTPUTS: During 2010 a Varian Inova 400MHz NMR spectrometer was acquired and installed in a refurbished room located in Gortner Laboratory of Biochemistry. The NMR instrument was used to obtain spectra used in the elucidation of chemical structure for the proton, carbon or fluorine atoms within the molecule. Over 1000 samples were run for research purposes. Data was given to individual research scientists located primarily for the St Paul campus. PARTICIPANTS: Research scientists on the St Paul campus were the primarily users of the instrumentation. TARGET AUDIENCES: NMR spectroscopy is important in understanding life processes at the molecular level with the results presented by the research scientists to their research areas. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts
Spectra obtained by NMR spectroscopy give scientists information on the composition of organic chemical compounds. This information can be used in a wide variety of research programs to help understand the chemistry underlying many biological questions.
Publications
- Heilmann SM, Jader LR, Harned LA, Sadowsky MJ, Schendel FJ, Lefebvre PA, von Keitz MG, Valentas KJ. (in press 2010) Hydrothermal carbonization of microalgae II. Fatty acid, char, and algal nutrient products. Applied Energy.
|
Progress 01/01/09 to 12/31/09
Outputs
OUTPUTS: The NMR instrument was used primarily to provide 300Mz proton spectra for chemical structure elucidation. Over the course of 2009 over 750 samples were run for research purposes. Data obtained by use of the instrumentation was given to individual scientists. The computer system operating this instrument has become unusable and the instrument was retired. Funding was obtained for a 400 MHz NMR and it was delivered in December 2009 and will be installed in January 2010. PARTICIPANTS: Research scientists on the St Paul campus were the main users of the instrumentation. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: The 300 MHz NMR which was purchased in 1981 was retired and a 400 MHz NMR was purchased and will be installed in 2010.
Impacts
Spectra obtained by NMR spectroscopy give scientists substructure information on the composition of organic chemical compounds. This information can be used in a wide variety of research programs to help understand the chemistry underlying many biological questions.
Publications
- No publications reported this period
|
Progress 01/01/08 to 12/31/08
Outputs
OUTPUTS: The NMR instrument was used primarily to provide 300Mz proton spectra for chemical structure elucidation. Over the course of 2008 over 650 samples were run for research purposes. Data obtained by use of the instrumentation was given to individual scientists primarily on the St Paul campus. The computer system operating this instrument has become unstable over the last year and would need replacing. PARTICIPANTS: Research scientists on the St Paul campus were the main users of this instrumentation. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: The computer system for the NMR instrumentation has become unstable and is in need of replacement. The instrument might need to be retired if the data system becomes unrepairable.
Impacts
Spectra obtained by NMR spectroscopy give scientists substructure information on the composition of organic chemical compounds. This information can be used in a wide variety of research programs to help understand the chemistry underlying many biological questions.
Publications
- No publications reported this period
|
Progress 01/01/07 to 12/31/07
Outputs
The 300 MHz NMR instrument was used primarily to provide proton spectra to researchers on the St Paul campus. During 2007, 515 samples were run on the NMR instrument. Data obtained would give complementary structural information to that obtained by mass spectrometry
Impacts
NMR spectroscopy is a well used techinique to give substructure information about the chemical makeup of various organic molecules. The instrument was used to provide analytical service to researchers on the St Paul campus.
Publications
- No publications reported this period
|
Progress 01/01/06 to 12/31/06
Outputs
The instrument was used primarily to provide service to researchers on the St Paul campus. The identity and makeup of various organic molecules are an integral part of a wide variety of research projects. During 2006 over 850 samples were run on the NMR instrument with Food Science and Nutrition as a primary user.
Impacts
The instrument was used primarily to provide service to researchers on the St Paul campus. The identity and makeup of various organic molecules are an integral part of a wide variety of research projects.
Publications
- Data obtained were used in graduate reaserch in 2006.
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Progress 01/01/05 to 12/31/05
Outputs
During the past year 997 samples were run on the 300Mz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer.The instrument was used primarily to provide service to researchers on the St Paul campus of the University of Minnesota. Proton and carbon spectra were obtained so as to obtain information on the chemical/structural makup of organic molecules. Typical results obtained by the use of NMR include identification of natural products, structural confirmation of biopolymers, determination of pathways and end products of microbial synthesis and information on industrial and agricultural pollutants.
Impacts
The instrument was used primarily to provide service to researchers on the St Paul campus. The identity and makeup of various organic molecules are an integral part of a wide variety of research projects.
Publications
- Analytical information was included in several publications in 2005.
|
Progress 01/01/04 to 12/31/04
Outputs
During the past year 607 samples were run on the 300MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument was used primarily to provide service to researchers on the St Paul campus of the University of Minnesota. Proton and carbon NMR spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Typical results obtained by the use of NMR include: identification of natural products, structural confirmation of biopolymers, determination of biochemical pathways and end products of microbial systhesis, information on industrial and agricultural pollutants and of lignin structure.
Impacts
This instrument was use primarily to provide a service to researchers on the St Paul campus. The identity and makeup of various organic molecules are an integral part of a wide variety of research projects.
Publications
- Analytical information obtained by NMR were included in several publication in 2004.
|
Progress 01/01/03 to 12/31/03
Outputs
During the past year 618 samples were run on the 300 Mz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer.This instrument was used primarily to provide service to researchers on the St Paul campus of the University of Minnesota. Proton anc carbon spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Typical results obtained by use of NMR include: identification of natural products, determination of biochemical pathways, information on degredation of industrial and agricultural pollutants, and carbohydrate or lignin structure.
Impacts
This instrument was use primarily to provide a service to researchers on the St Paul campus. The identity and makeup of various organic molecules are an integral part of a wide variety of research projects.
Publications
- Analytical information obtained from NMR were included in several publication in 2003.
|
Progress 01/01/02 to 12/31/02
Outputs
During the past year 897 samples were run on the 300MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument was used primarily to provide service to researchers on the St Paul campus. Proton, carbon and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs Rex Lovrien, Larry Wackett (Biochemistry), John Gronwald (Agronomy), Fredrich Srienc (BTI), Simo Sarkanen ( Wood and Paper Science). Typical results obtained by use of NMR spectroscopy include: identification of natural products, makeup of lignin structure, determination of biochemical pathways, structural identification of plant and fungal toxins, information on microbial degradation of industrial and agricultural pollutants and carbohydrate structure.
Impacts
This instrument is used primarily to provide service to researchers on the St Paul Campus. Proton, carbon and phosphorous spectras were obtained so as to information on the chemical structure of organic molecules. The identity and makeup of these molecules are an integral part of the varied research projects.
Publications
- Data from this lab were included in several publications in 2002.
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Progress 01/01/01 to 12/31/01
Outputs
During the past year 781 samples were run on the 300 Mhz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument is used primarily to provide service to researchers on the St Paul Campus. Proton, deuterium, carbon and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs Rex Lovrien , Larry Wackett and Bridgette Barry (Biochemistry), John Gronwald (Agronomy), Fredrich Srienc (BTI), Simo Sarkanen, Ulrike Tschirner and Steve Severtson (Wood and Paper Sciences). Typical results obtained by use of NMR spectroscopy include: identification of natural products, makeup of lignin structure, determination of biochemical pathways, structural identification of plant and fungal toxins, information on microbial degradation of industrial and agricultural pollutants and carbohydrate structure.
Impacts
This instrument is used primarily to provide service to researchers on the St Paul Campus. Proton, deuterium, carbon and phosphorous spectra were obtained so as to get information on the chemical structure of organic molecules. The identity and makeup of these molecules are an integral part of the varied research projects.
Publications
- Data obtained form this lab were included in several publications and theses in 2001.
|
Progress 01/01/00 to 12/31/00
Outputs
During the past year 995 samples were run on the 300 MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument is used primarily to provide service to researchers on the St. Paul Campus. Proton, deuterium, carbon, and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs. Rex Lovrien, Larry Wackett (Biochemistry), Dave Somers (Agronomy), David Sherman (Biological Process Institute), Florence Gleason (Plant Biology), M.J. Sadowsky (Soils), Simo Sarkanen and Ulrike Tschirner (Forest Products). Typical results obtained by use of NMR spectroscopy include: identification of flavor components, makeup of lignin structure, determination of biochemical pathways, structural identification of plant and fungal toxins, information on the microbial degradation of industrial and agricultural pollutants, carbohydrate structure, and the elucidation of organic
structures of natural products.
Impacts
This instrument is used primarily to provide service to researchers on the St. Paul Campus. Proton, deuterium, carbon, and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules.
Publications
- Data obtained from this lab were included in many publications as well as several theses in 2000.
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Progress 01/01/99 to 12/31/99
Outputs
During the past year 1224 samples were run on the 300 MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument is used primarily ot provide service to researchers on the St. Paul Campus. Proton, deuterium, carbon, and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs. Rex Lovrien, Larry Wackett (Biochemistry), Dave Somers (Agronomy), David Sherman, Fredrich Srienc, Michael Flickinger, Dan Urry (Biological Process Institute), Chester Mirocha (Plant Pathology), M.J. Sadowsky (Soils), Ron Vesonder (USDA), Simo Sarkanen and Ulrike Tschirner (Forest Products). Typical results obtained by use of NMR spectroscopy include: identification of flavor components, makeup of lignen structure, determination of biochemical pathways, structural identification of plant and fungal toxins, information on the microbial degradation of industrial and agricultural
pollutants, carbohydrate structure, and the elucidation of organic structures of natural products.
Impacts
This instrument is used primarily to provide service to researchers on the St. Paul Campus. Proton, deuterium, carbon, and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules.
Publications
- Data obtained from this lab were included in many publications as well as several theses in 1999.
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Progress 01/01/98 to 12/31/98
Outputs
During the past year over 1300 samples were run on the 300 MHz Nicolet Nuclear Magnetic Resonance - NMR - spectrometer. This instrument is used primarily to provide service to researchers on the St. Paul Campus. Proton, deuterium, carbon, and phosphorus spectra were obtained so as to get information on the chemical-structural makeup of organic molecules. Researchers making use of the NMR include: Drs. Rex Lovrien, Larry Wackett, Norma Allewell - Biochemistry; Dr. Gary Reineccius - Food Science; Drs. David Sherman, Friedrich Srienc, Michael Flickinger - Biological Process Institute; Dr. Chester Mirocha - Plant Pathology; Dr. M.J. Sadowsky - Soils; Drs. Simo Sarkanen and Ulrike Tschirner - Forest Products. Typical results obtained by use of NMR spectroscopy include: identification of flavor components, makeup of lignin structure, determination of biochemical pathways, structural identification of plant and fungal toxins, information on the microbial degradation of
industrial and agricultural pollutants, carbohydrate structure, and the elucidation of organic structures of natural products.
Impacts
(N/A)
Publications
- Data obtained from this lab were included in many publications as well as several theses in 1998.
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Progress 01/01/97 to 12/31/97
Outputs
During the past year over 1400 samples were run on the 300 MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument is used primarily to provide service to researchers on the St. Paul Campus. Proton, deuterium, carbon, and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs. Larry Wackett, Michael Raftery (Biochemistry); Dr. Dave Somers (Agronomy), Dr. Gary Reineccius (Food Science); Drs. David Sherman, Friedrich Srienc (Biological Process Institute); Dr. Chester Mirocha (Plant Pathology); Dr. M.J. Sadowsky (Soils); Drs. Simo Sarkanen, Ulrike Tschirner (Forest Products); and Dr. Alan Hooper (Genetics/Cell Biology). Typical results obtained by use of NMR spectroscopy include: identification of flavor components, makeup of lignen structure, determination of biochemical pathways, structural identification of plant and fungal toxins, information
on the microbial degradation of industrial and agricultural pollutants, carbohydrate structure, and the elucidation of organic structures of natural products.
Impacts
(N/A)
Publications
- Data obtained from this lab were included in many publications as well as several theses. 1997.
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Progress 01/01/96 to 12/30/96
Outputs
During the past year over 1400 samples were run on the 300 MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument is used primarily to provide service to researchers on the St. Paul Campus. Proton, deuterium, carbon, fluorine and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs. Rex Lovrien, Larry Wackett, Victor Bloomfield, Gary Nelsestuen (Biochemistry); Dr. Dave Somers (Agronomy), Dr. Gary Reineccius (Food Science); Dr. Mark Brenner, (Horticulture); Drs. David Sherman, Friedrich Srienc (Biological Process Institute; Dr. Chester Mirocha (Plant Pathology); Dr. M.J. Sadowsky (Soils); Dr. Simo Sarkanen (Forest Products); Dr. Robert Brambl (Plant Biology), Dr. Mrinal Bhattacharya (Agricultural Engineering) and Dr. Alan Hooper (Genetics/Cell Biology). Typical results obtained by use of NMR spectroscopy include: identification of flavor
components, makeup of lignen structure, determination of biochemical pathways, structural identification of plant and fungal toxins, information on the microbial degradation of industrial and agricultural pollutants, carbohydrate structure, and the elucidation of organic structures of natural products.
Impacts
(N/A)
Publications
- Data obtained from this lab were included in many publications as well as several theses.
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Progress 01/01/95 to 12/30/95
Outputs
During the past year over 1700 samples were run on the 300 MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument is used primarily to provide service to researchers on the St. Paul Campus. Proton, deuterium, carbon, fluorine and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs. Rex Lovrien, Larry Wackett, Clare Woodward (Biochemistry); Dr. Dave Somers (Agronomy), Dr. Gary Reineccius (Food Science); Drs. Mark Brenner, Cindy Tong, Bud Markhart (Horticulture); Drs. David Sherman, Friedrich Srienc (Biological Process Institute); Dr. Chester Miocha (Plant Pathology; Dr. M.J. Sadowsky (Soils); Dr. Simo Sarkanen (Forest Products); Drs. Florence Gleason, Robert Brambl (Plant Biology), Dr. Mrinal Bhattacharya (Agricultural Engineering) and Dr. Alan Hooper (Genetics/Cell Biology). Typical results obtained by use of NMR spectroscopy include:
identification of flavor components, determination of biochemical pathways, structural identification of plant toxins, information on the microbial degradation of industrial pollutants, carbohydrate structure, and the elucidation of organic structures of natural products.
Impacts
(N/A)
Publications
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Progress 01/01/94 to 12/30/94
Outputs
During the past year over 1500 samples were run on the 300 MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument is used primarily to provide service to researchers on the St Paul Campus. Proton, deuterium, carbon, flourine and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs. Rex Lovrien, Larry Wackett (Biochemistry); Drs. Eugenia Davis, Gary Reineccius (Food Science); Dr. Mark Brenner (Horticulture); Drs. David Sherman, Friedrich Srienc (Biological Process Institute);Dr. Chester Mirocha (Plant Pathology): Drs. Peter Graham, M.J. Sadowsky (Soil Science); Dr. Simo Sarkanen (Forest Products); Dr. Florence Gleason (Plant Biology); and Dr. Alan Hooper (Genetics/Cell Biology). Typical results obtained by use of NMR spectroscopy include: identification of plant hormones, structural properties of lignin, determination of biochemical
pathways, structure of plant toxins, information on the microbial degradation of industrial pollutants, carbohydrate structure, and the elucidation of the organic structure of natural products.
Impacts
(N/A)
Publications
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Progress 01/01/93 to 12/30/93
Outputs
During the past year over 1400 samples were run on the 300 MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument is used primarily to provide service to researchers on the St. Paul Campus. Proton, deuterium, carbon, fluorine and phosphorous spectra were obtained so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs. Janet Schottel, Rex Lovrien, Larry Wackett (Biochemistry); Drs. Eugenia Davis, Paul Addis, R.G. Fulcher, and H.W. Schafer (Food Science); Dr. Mark Brenner (Horticulture); Drs. David Sherman, Friedrich Srienc (Biological Process Institute); Dr. Chester Mirocha (Plant Pathology); Drs. Peter Graham and M.J. Sadowsky (Soils); Drs. Chang Ho-Park and M. Bhattacharya (Agricultural Engineering); and Dr. Alan Hooper (Genetics/Cell Biology). Typical results obtained by use of NMR spectroscopy include: structural identification of insect pheromones, determination of
biochemical pathways, structural identification of plant toxins, information on the microbial degradation of industrial pollutants, carbohydrate structure, and the elucidation of organic structures of natural products.
Impacts
(N/A)
Publications
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Progress 01/01/92 to 12/30/92
Outputs
During the past year over 1900 samples were run on the 300 mHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer. This instrument is used primarily to service researchers on the St Paul Campus. It has the capability of running proton, deuterium, carbon, flourine and phosphorous NMR so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Drs. Janet Schottel, Michael Raftery, Rex Lovrien, Larry Wackett (Biochemistry), Drs. Eugenia Davis, Paul Addis, R.G. Fulcher, H.W. Schafer and Gary Reineccius (Food Science), Dr. Richard Jones (Entomology), Dr. Larry Felice (Veterinary Medicine), Drs. Michael Flickenger, David Sherman, Friedrich Srienc (Biological Process Institute), Dr. Chester Mirocha (Plant Pathology), Dr. J.W. Gronwald (Agronomy), Dr. Peter Graham and Dr. M.J. Sadowsky (Soils), Drs. Chang Ho-Park and M. Bhattacharya (Agricultural Engineering), and Dr. Alan Hooper (Genetics Cell Biology).
Typical results obtained by use of NMR spectroscopy include: structural identification of insect pheromones, determination of biochemical pathways, structural identification of plant toxins, information on the microbial degradation of industrial pollutants, carbohydrate structure, and the elucidation of organic structures of natural products.
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Progress 01/01/91 to 12/30/91
Outputs
A 300 mHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer is used primarily to service researchers on the St. Paul Campus. It has the capability of running proton, deuterium, carbon, flourine and phosphorous NMR so as to get information on the chemical/structural makeup of organic molecules. Researchers making use of the NMR include: Dr. Florence Gleason (Plant Biology), Dr. Simo Sarkanen (Forestry), Drs. John Anderson, Michael Raftery, Rex Lovrien (Biochemistry), Drs. Eugenia Davis, Paul Addis and Gary Reineccius (Food Science), Dr. Richard Jones (Entomology), Dr. Larry Felice (Veterinary Medicine), Drs. Michael Flickenger, Peter Carr, Friedrich Srience (Biological Process Institute), Dr. H. Nagasawa (VA), Drs. Larry Wackett and Luke Lam (Gray Freshwater Biological Institute), Dr. Chester Mirocha (Plant Pathology), Dr. J.W. Gronwald (Agronomy), Dr. Hamed Abbas (USDA), and Dr. Alan Hooper (Genetics Cell Biology). Typical results obtained by use of NMR
spectroscopy include: structural identification of insect pheromones, determination of biochemical pathways, structural properties of lignin, information on the microbial degradation of industrial pollutants, carbohydrate structure, and the elucidation of organic structures of natural products. Data obtained from this lab were included in many publications as well as several theses.
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Progress 01/01/90 to 12/30/90
Outputs
A 300 MHz Nicolet Nuclear Magnetic Resonance (NMR) spectrometer is used primarily to service researchers on the St. Paul Campus. It has the capability of running proton, deuterium, carbon, flourine and phosphorous NMR so as to get information on the chemical/structural makeup of organic molecules. The major user of the facility include: Dr. Florence Gleason (Plant Biology), Dr. Simo Sarkanen (Forestry), Dr. John Anderson (Biochemistry) Drs. Eugenia Davis and Gary Reineccius (Food Service) and Dr. Richard Jones (Entomology). Other Users include: Drs. Michael Raftery, David Bernlohr, T.Y. Tsong, Gary Nelsestuen and Clare Woodward (Biochemistry), Dr. Larry Felice (Veterinary Medicine), Dr. Michael Flickinger (Biological Process Institute), Dr. H. Nagasawa (VA), Drs. Larry Wackett, Luke Lam and John Wood (Gray Freshwater Biological Institute and Dr. Chester Mirocha (Plant Pathology). Results obtained by use of NMR spectroscopy include: structural identification of
insect pheromones, determination of biochemical pathway of microbial synthesis, structural properties of lignin, information on the microbial degradation of industrial pollutants, carbohydrate structure, and organic makeup of natural products.
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Progress 01/01/89 to 12/30/89
Outputs
The Nuclear Magnetic Resonance (NMR) facility on the St. Paul Campus consists ofa 300 megaHertz Nicolet NT-300-WB NMR spectrometer and an off-line GE data station. The facility was used primarily to service researchers on the St. Paul Campus. Routine capabilities include running proton, deuterium, carbon and phosphorous NMR to get chemical-structural information on organic molecules. The major users of the facility include: Drs. Eugenia Davis and Gary Reineccius (Food Science), Dr. Clare Woodward (Biochemistry), and Dr. Simo Sarkanen (Forestry). Other users include: Drs. Florence Gleason and Al Frenkel (Plant Biology), Dr. Joe Warthesen (Food Science), Drs. Brian Ross, Larry Wackett and Luke Lam (Gray Freshwater Biological Institute), Drs. Victor Bloomfield, Michael Raftery, David Bernlohr and John Anderson (Biochemistry), Dr. Peter Graham (Soils), Dr. Chester Mirocha (Plant Pathology) and Dr. Richard Jones (Entomology). Results obtained by use of NMR spectroscopy
include: chemical structure of foods baked by conventional vs. microwave ovens. Intracellular pH of soil microbes. Flavor components identification. Hydrogen exchange kinetics of assigned NH resonances in BPTI. The structure and physiocochemical properties of lignin. Structure of naturally occurring mycotoxins. Identification of insect pheromones. Microbial degradation products from industrial pollutants. Organic structure analysis in natural products.
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Progress 01/01/88 to 12/30/88
Outputs
During the past year, a Nicolet 300 MH(Z) nuclear magnetic resonance (NMR) spectrometer was the primary instrument used to service researchers on the St. Paul campus. The facility includes capabilities for routinely doing proton, deuterium, carbon and phosphorous NMR along with an off-line data processing capability. The major users of the facility were: Dr. Eugenia Davis (Food Science), Drs. Clare Woodward and Victor Bloomfield (Biochemistry) and Dr. Simo Sarkanen (Forestry). A number of shorter term users include faculty from Soil Science, Agronomy, Plant Pathology, Fisheries and Wildlife, Entomology, Biological Process Technology, Food Science, Biochemistry, and Gray Freshwater Biological Institute. Results obtained this past year include the following. The hydrogen exchange kinetics of assigned peptide NH resonances by 'H NMR spectroscopy in bovine paracreatic trypsin inhibitor were characterized. Soil microbes were studied. The structure and
physiocochemical properties of lignin derivatives were investigated. Binding sites in bovine prothrombin were identified. The effects of polyamine binding on the hydrogen exchange and structural transitions of DNA were studied. Chemical structure of foods baked with microwave vs conventional ovens were studied. Additionally, NMR techniques were used to analyze other natural products such as mycotoxins, plant hormones, carbohydrates, insect phermones, steroids and organic acids.
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Progress 01/01/87 to 12/30/87
Outputs
During the last year, the Nicolet 300 MH(z) nuclear magnetic resonance (NMR) spectrometer has been scheduled and used approximately 340 days. An off-line data processing station was added to the facility and has significantly increased instrument time available for experiments. The major users of the facility were Dr. Simo Sarkanen (Forest Products) and Drs. Clare Woodward and Victor Bloomfield (Biochemistry). A large number of shorter term users include faculty from Entomology, Botany, Biochemistry, Fisheries and Wildlife, Plant Pathology, Agronomy, Biological Process Technology, Food Science, Forestry and Gray Freshwater Biological Institute. Results obtained in the past year include the following. The identification of calcium ion binding site and a movel acetylation site of metal ion binding in bovine prothrombin protein were made. The structure and physicochemical properties of lignin derivatives were investigated by H and C NMR. The hydrogen exchange kinetics
of assigned peptide NH resonances by H NMR spectroscopy in bovine pancreatic trypsin inhibitor were characterized. The effects of polyamine binding on the hydrogen exchange and structural transitions of oligomeric and polymeric DNA were studied. Additionally, mycotoxins, carbohydrates, insect pheromones, plant hormones, organic acids, steroids or other natural products have been analyzed using either H, 13C or P NMR.
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Progress 01/01/86 to 12/30/86
Outputs
During the last year of Nicolet 300MHz nuclear magnetic resonance (NMR) spectrometer has been scheduled and used greater than 90% of the time. A new data station has been added to the facility this year. Long term projects were carried out by D. Simo Sarkanen (Forest Products) and Drs. Clare Woodward and Victor Bloomfield (Biochemistry). A large number of short term users include faculty from Food Science, Veterinary Medicine, Plant Pathology, Biochemistry, Forest Products, Genetics and Cell Biology, Botony and Gray Freshwater Biological Institute. Results obtained in the past year include the following. The structure and physicochemical properties of lignin derivatives were investigated by H and C NMR. The hydrogen exchange kinetics of assigned peptide NH resonances by H NMR spectroscopy in bovine pancreatic trypsin inhibitor were characterized. The effects of polyamine binding on the hydrogen exchange and structural transitions of oligomeric and polymeric DNA
were studied by H and P NMR. Additionally, the chemistry of mycotoxins, sterols, algae, carbohydrates, insect pheromones, plant hormones and other materials have been studied using either H, C or P NMR.
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Progress 01/01/85 to 12/30/85
Outputs
During the last year the Nicolet 300 MHz nuclear magnetic resonance (NMR) spectrometer funded by this grand has been scheduled and used 24 hours per day, seven days per week. Long term projects were carried out by Dr. Simo Sarkanen of the Department of Forest Products, and Drs. Clare Woodward and Victor Bloomfield of the Department of Biochemistry. A large number of short term users include faculty in Food Science, Plant Pathology, Forest Products, Biochemistry, and Genetics and Cell Biology. For the short term users in the Agriculture Institute, spectra of samples provided by the users are obtained by experienced NMR spectroscopist, who is also available for consultation on planning experiments and for interpretation of the spectra. Results obtained in the past year include the following. The hydrogen exchange kinetics of assigned peptide NM resonances by H NMR spectroscopy in bovine pancreatic trypsin inhibitor were characterized, both with and without complex
formation with trypsin and trypsinogen. The chemistry of lignin derivatives was investigated by H and C NMR. The effects of polyamine binding on the hydrogen exchange and structural transitions of oligomeric and polymeric DNA has been studied by H and P NMR. In addition, the chemistry of tannins has been studied by H and P NMR, tocophenols by H NMR, the chemistry of mycotoxins by H and C NMR.
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Progress 01/01/84 to 12/30/84
Outputs
This grant funds part of the operating expenses of the NMR facility located in the Biochemistry Department of the College of Biological Sciences. This facility provides high resolution NMR capability free of charge to Institute of Agriculture and Biochemistry Department faculty. Dr. Woodward (Biochemistry Dept.) has an ongoing NMR project concerned with the internal motions of globular proteins as measured by the hydrogen isotope exchange of peptide amide NH protons in proteins. The proteins studied by Woodward are trypsin inhibitor and the complex of trypsin and trypsin inhibitor. Dr. Bloomfield (Biochemistry Dept.) is studying the interactions of oligonucleotides with polyamines as measured by the effect of polyamines on he hydrogen isotope exchange of oligonucleotide amino protons. Dr. Sarkanen (Agriculture Inst.) is uing PGH NMR to chemically characterize the individual molecular species in lignins formed during the kraft pulping process. Dr. T. Labuza (Agric.
Inst.) is using NMR to determine the metabolites and shelf breakdown products of aspartame, the sweetner marketed under the trade names Nutrasweet and Equal. Dr. C. Mirocha (Agric. Inst.) has used NMR for the identification of mycotoxins and their metabolites. Dr. G. Reineccius (Agric. Inst.) has used the NMR facility for characterization of flavo compounds.
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Progress 01/01/83 to 12/30/83
Outputs
Gander Project: Pencicillium species secrete phosphocholine-phoethanamine-containing glycopeptides if the medium is below pH 4 and phosphoglycoenzymes if the pH is above 5. The enzymes are being anayzed by NMR spectroscopy: they are all lytic enzymes which degrade carbohydrates and phosphorylated substances. None are proteases. The pH of the medium increases as carbohydrate is depleted and organic acids are taken up. The function of the phosphocholine-/phosphoethanolamine-containing regions are under investigation. Woodward Project: Buried, labile H atoms of proteins undergo isotopic exchange with solvent H atoms. This indicates that the compact, tightly packed protein structure undergoes internal fluctuations that expose buried sites to solvent. This inherent flexibility of proteins is thought to be a fundamental aspect of the biological activity of proteins. Hydrogen isotope exchange studies can be employed to characterize these motions. We use NMR
spectroscopy to determine the hydrogen exchange kinetics of assigned H atoms. Nelsestuen Project: Research has involved the vitamin K-dependent proteins and their unique calcium-binding functions. Lanthanide ions which are paramagenetic and broaden adjacent signals were substituted for calcium ions. Bound lanthanides were measured by monitoring lanthanide-induced changes in buffer signals.
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Progress 01/01/82 to 12/30/82
Outputs
The 13C and 31P nuclear magnetic resonance spectroscopy has shown that a new class of fungal extracellular hydrolases are glycoproteins which contain phosphoethanolamine and phosphocholine residues covalently attached to a large galactomannan. These hydrolases degrade polysaccharides and phosphorylated substances in the fungi's environment. The role of the phosphoethanolamine and phosphocholine is being sought. The 13C NMR spectroscopy has shown that the structure of the extracellular polysaccharide secreted by three strains of Yogurt producing bacteria are identical, although the percentage of galactose, glucose and mannose are different in each polymer. These polysaccharides have properties which influence the texture of yogurt. Proton NMR spectroscopy has shown that cooperative metal ion binding occurs in a prothrombin phospholipid system. The role of metal ions in the blood clotting process is under investigation. Proton NMR spectroscopy is being used to
measure the rate of exchange of peptide NH protons with those in water. Protons buried in native proteins exchange slowly; the exchange rate increases as the protein unfolds. The rate of exchange of those proteins whose chemical shifts are located under the main aromatic envelope of the spectrum have been investigated as a function of pH and temperature. Proton NMR spectra of oligo- and polysaccharides useful in identifying N-acetylamannosamine and N-acetylmannosaminuronic acid have been found.
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Progress 01/01/81 to 12/30/81
Outputs
Nuclear Magnetic Resonance (NMR) spectroscopy is useful in establishing the structure and concentrations of substances found in living systems. This technique is also used to follow the concentrations of metabolites in vivo. An NMR spectroscopy laboratory was established in the Department in August with a 70.5 KGauss superconducting NMR spectrometer with Fourier transform capability attached to a computer with 128 K of memory. A period of 2 to 3 months was required for the operator to become familiar with the operation and capability of the instrument. During the past two months the instrument has been used to determine the structures of samples provided by scientists in the Departments of Biochemistry, Entomology and Plant Pathology. These samples represent substances in the categories of polysaccharides, proteins, toxins, degradation products from bacteria and insecticides. We anticipate an increasing use of this facility as scientists doing agricultural
research become more familiar with the wide variety of ways in which an NMR spectrometer may be used in their specific research programs.
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