TRANSMISSION ELECTRON MICROSCOPE SERVICE FACILITY

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
404	2499	2010	65%
404	3999	2010	35%

Progress 07/01/74 to 09/30/18

Outputs
Target Audience:Main audience is Kansas State University faculty, graduate students, undergraduate students and researcher; also include Board of Reagents School, state K-12 schools, USDA, and businesses. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?What opportunities for training and processional development has the project provided? a) Individual or group training on K-State Microscopy Facility Equipment. Undergraduates (75) Graduates students (95) Postdoctoral fellows (12) Faculty (38) Industry (8) USDA (3) b) Demos, workshops, instruction: Industry (6 individuals) (3 hr 20 min.) 1) Lecture: A. List courses taught this year. Identify fall, spring semester, and include summer school and intersession courses. 1) Lecture: (If the course was co-taught, who was the coordinator?) Your Contact Approximate Course No. Title Hours/Semester No. of Students Biol 886 Confocal, Fluor & Light Microscopy 19 7 Biol 888 Electron Microscopy 12 8 2) Laboratory: Your Contact Approximate Course No. Title Hours/Semester No. of Students Biol 886 Confocal, Fluor & Light Microscopy 56.7 7 a) Lab Coordinator/Supervisor (name staff) Daniel Boyle b) Lab Sections you personally delivered: ALL Biol 495 TOP/Phage Hunters Part 1 5.4 21 a) Lab Coordinator/Supervisor (name staff) Martha Smith Caldas, Chris Herren b) Lab Sections you personally delivered: ALL Imaging of Phages Biol 888 Electron Microscopy 20 8 a) Lab Coordinator/Supervisor (Daniel Boyle) b) Lab Sections you personally delivered: All FDSCI 601 Food Microbiology Lab 5.4 60 a) Lab Coordinator/Supervisor (Daniel Boyle) Class instructor Valentina Trinetta b) Lab Sections you personally delivered: one Active extramural grants (title, granting period, amount, source or agency) Biol 890 Advanced Topics in Electron Microscopy: Global Campus 2017 Summer School mini-grant covering hotel, travel, and UNL microscope fees (~$10,000) for course. Funded Title: Center of Biomedical Research Excellence (COBRE) in Protein Structure and Function. Director: Robert P. Hanzlik, Ph.D.; Program Assistant: Cynthia Beall. Granting agency: National Institute of Health. Dates: 04/01/20013 until 2017. Amount: Five-year competitive renewal total cost. Resource: Division of Biology Microscopy Facility, Daniel Boyle. How have the results been disseminated to communities of interest?How have the results been disseminated to communities of interest? Publications- For further information on specific experiments, publications and grants, primary investigators will have to be consulted. 1) Manuscripts published or in press in journals, books, chapters, etc. Indicate those that are peer reviewed. Indicate undergraduate authors/co-authors by asterisk. *Maduraimuthu Djanaguiraman; Dan Boyle; Ruth Welti; Krishna Jagadish; P.V. Vara Prasad. (2018) Decreased photosynthetic rate under high temperature in wheat is due to lipid desaturation, oxidation, acylation and damage of organelles BMC Plant Biology. 18:55-77. *Alex R. Wilden, Joshua A. Molina, Melissa Feuerborn, Daniel Boyle, and Stella y. Lee. (2018) Glutamine-dependent lysosome homeostatic changes induced by starvation and lysosome inhibition. BBA - Molecular Cell Research. 1865: 1356-1367. *Vijay K. Eedunuri, Yuntao Zhang, Chuanmin Cheng, Li Chen, Huitao Liu, Anders Omsland, Dan Boyle, and Roman R. Ganta. (2018) Protein and DNA synthesis demonstrated in cell-free Ehrlichia chaffeensis organisms in axenic medium. Scientific Reports. 8: Article number: 9293. What do you plan to do during the next reporting period to accomplish the goals?What do you plan to do during the next reporting period to accomplish the goals? Continue with current strategy to accomplish goals.

Impacts
What was accomplished under these goals? What was accomplished under these goals? Information and data obtained from this facility was incorporated into peer review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science. The Microscopy Facility and the expertise of Dr. Boyle was also vital to the education, research, and services of KSU faculty, students and researchers as well as industry and other Universities. Clearly, this facility has been a valuable instrument for promoting the broad K-State goals of education, research, and service. The Kansas State University, Biology Microscopy Facility Contacts Phone (600) E-mail received (7,171) E-mail sent (938) Number of individuals and labs given training or technical assistance: faculty (38) Graduate students and Postdocs (55) Undergraduates (95) Industry (8) USDA (3) g) Departments and others using facility or services: The Microscopy Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as Fort Hays State University, USDA (Manhattan), University of Nebraska Lincoln, University of Bristol UK, American Oil Chemists Society (AOCS) and businesses such as Cargill, Nestle, Chipotle, Cool Springs International, LLC, Vita Terra Sustainability and Lortscher Animal Nutrition, Bern, KS.

Publications

Type: Journal Articles Status: Published Year Published: 2018 Citation: *Maduraimuthu Djanaguiraman; Dan Boyle; Ruth Welti; Krishna Jagadish; P.V. Vara Prasad. (2018) Decreased photosynthetic rate under high temperature in wheat is due to lipid desaturation, oxidation, acylation and damage of organelles BMC Plant Biology. 18:55-77. *Alex R. Wilden, Joshua A. Molina, Melissa Feuerborn, Daniel Boyle, and Stella y. Lee. (2018) Glutamine-dependent lysosome homeostatic changes induced by starvation and lysosome inhibition. BBA Molecular Cell Research. 1865: 1356-1367. *Vijay K. Eedunuri, Yuntao Zhang, Chuanmin Cheng, Li Chen, Huitao Liu, Anders Omsland, Dan Boyle, and Roman R. Ganta. (2018) Protein and DNA synthesis demonstrated in cell-free Ehrlichia chaffeensis organisms in axenic medium. Scientific Reports. 8: Article number: 9293.

Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Target Audience Main audience is Kansas State University faculty, graduate students, undergraduate students and researcher; also include Board of Reagents School, state K-12 schools, USDA, and businesses Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?What opportunities for training and processional development has the project provided? a) Individual or group training on K-State Microscopy Facility Equipment. Undergraduates (75) Graduates students (55) Postdoctoral fellows (12) Faculty (38) Industry (8) USDA (3) b) Demos, workshops, instruction HRTEM, EELS: University of Nebraska Lincoln (2 individuals) (3 hr 20 min.) 1) Lecture: A. List courses taught this year. Identify fall, spring semester, and include summer school and intersession courses. 1) Lecture: (If the course was co-taught, who was the coordinator?) Your Contact Approximate Course No. Title Hours/Semester No. of Students Biol 886 Confocal, Fluor & Light Microscopy 31 7 Biol 890 Advanced Topics Electron Microscopy 10 14 (10 KSU, 4 UNL) 2) Laboratory: Your Contact Approximate Course No. Title Hours/Semester No. of Students Biol 886 Confocal, Fluor & Light Microscopy 76.7 7 a) Lab Coordinator/Supervisor (name staff) Daniel Boyle b) Lab Sections you personally delivered: ALL Biol 495 TOP/Phage Hunters Part 1 5.4 10 a) Lab Coordinator/Supervisor (name staff) Martha Smith Caldas, Chris Herren b) Lab Sections you personally delivered: ALL Imaging of Phages Biol 890 Advance Topics Electron Microscopy 20 14 (10 KSU, 4 UNL) a) Lab Coordinator/Supervisor (Daniel Boyle) b) Lab Sections you personally delivered: All FDSCI 601 Food Microbiology Lab 5.1 60 a) Lab Coordinator/Supervisor (Daniel Boyle) Class instructor Valentina Trinetta b) Lab Sections you personally delivered: one How have the results been disseminated to communities of interest?How have the results been disseminated to communities of interest? Publications- For further information on specific experiments, publications and grants, primary investigators will have to be consulted. 1) Manuscripts published or in press in journals, books, chapters, etc. Indicate those that are peer reviewed. Indicate undergraduate authors/co-authors by asterisk. 1) Authors: Maduraimuthu Djanaguiraman; Dan Boyle; Ruth Welti; Krishna Jagadish; P.V. Vara Prasad, Ph.D. Corresponding author: Prof. P.V. Vara Prasa Title: Decreased photosynthetic rate under high temperature in wheat is due to lipid desaturation, oxidation, acylation and cell organelles damage. Journal: BMC Plant Biology (submitted) 2) Authors: Gagan Gandhi, Jayendra K. Amamcharla, Dan Boyle Title: Effects of milk protein concentrate (MPC80) quality on susceptibility to fouling during thermal processing. Journal: Food Science and Technology Volume 81,August 2017, Pages 170-179 * What do you plan to do during the next reporting period to accomplish the goals?Continue with current strategy to accomplish goals.

Impacts
What was accomplished under these goals? Information and data obtained from this facility was incorporated into peer review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science. The Microscopy Facility and the expertise of Dr. Boyle was also vital to the education, research, and services of KSU faculty, students and researchers as well as industry and other Universities. Clearly, this facility has been a valuable instrument for promoting the broad K-State goals of education, research, and service. The Kansas State University, Biology Microscopy Facility Contacts Phone (611) E-mail received (8,059) E-mail sent (938) Number of individuals and labs given training or technical assistance: faculty (38) Graduate students and Postdocs (55) Undergraduates (75) Industry (8) USDA (3) g) Departments and others using facility or services: The Microscopy Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as Fort Hays State University, USDA (Manhattan), University of Nebraska Lincoln, University of Bristol UK, American Oil Chemists Society (AOCS) and businesses such as Cargill, Nestle, Chipotle, Cool Springs International, LLC, Vita Terra Sustainability and Lortscher Animal Nutrition, Bern, KS. ?

Publications

Type: Journal Articles Status: Submitted Year Published: 2017 Citation: 1) Authors: Maduraimuthu Djanaguiraman; Dan Boyle; Ruth Welti; Krishna Jagadish; P.V. Vara Prasad, Ph.D. Corresponding author: Prof. P.V. Vara Prasa Title: Decreased photosynthetic rate under high temperature in wheat is due to lipid desaturation, oxidation, acylation and cell organelles damage. Journal: BMC Plant Biology (submitted)
Type: Journal Articles Status: Published Year Published: 2017 Citation: Authors: Gagan Gandhi, Jayendra K. Amamcharla, Dan Boyle Title: Effects of milk protein concentrate (MPC80) quality on susceptibility to fouling during thermal processing. Journal: Food Science and Technology Volume 81, August 2017, Pages 170-179 *

Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Main audience is Kansas State University faculty, graduate students, undergraduate students and researcher; also include Board of Reagents School, state K-12 schools, USDA, and businesses. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?a) Individual or group training on K-State Microscopy Facility Equipment. Undergraduates (21) Graduates students (55) Postdoctoral fellows (10) Faculty (40) Industry (6) USDA (3) b) Demos, workshops, instruction and tour of microscopes: Industry (5 individuals) (1 hr 20 min.) 1) Lecture: Contact Approximate Course No. Title Hours/Semester No. of Students Biol 888 (Fall) Electron Microscopy 30 8 2) Laboratory: Contact Approximate Course No. Title Hours/Semester No. of Students Biol 888 Electron Microscopy 62 8 a) Lab Coordinator/Supervisor (name staff) Daniel L Boyle b) Lab Sections you personally delivered: All Biol 495 TOP/Phage Hunters Part 1 3 20 a) Lab Coordinator/Supervisor (name staff) Martha Smith Caldas, Chris Herren b) Lab Sections you personally delivered: ALL Imaging of Phages How have the results been disseminated to communities of interest?Publications- For further information on specific experiments, publications and grants, primary investigators will have to be consulted. What do you plan to do during the next reporting period to accomplish the goals?Continue with current strategy to accomplish goals.

Impacts
What was accomplished under these goals? Information and data obtained from this facility was incorporated into peer review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science. Clearly, this facility has been a valuable instrument for promoting the broad K-State goals of education, research, and service. The Kansas State University, Biology Microscopy Facility Contacts Phone (411) E-mail received (6898) E-mail sent (928) Number of individuals and labs given training or technical assistance: faculty (40) Graduate students and Postdocs (65) Undergraduates (21) Industry (6) USDA (3) g) Departments and others using facility or services: The Microscopy Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as Fort Hays State University, USDA (Manhattan), American Oil Chemists Society (AOCS) and businesses such as Cargill, Nestle, Chipotle, Cool Springs International, LLC, and Lortscher Animal Nutrition, Bern, KS.

Publications

Type: Journal Articles Status: Published Year Published: 2016 Citation: Vijay K. Eedunuri, Chuanmin Cheng, Anders Omsland, Dan Boyle, and Roman R. Ganta. (2016) Metabolic and biosynthetic activity of Ehrlichia chaffeensis in a host cell-free medium. 28th Annual American Society for Rickettsiology (ASR). June 11-14, Big Sky, MT.
Type: Other Status: Under Review Year Published: 2016 Citation: Donghun Kim, Joshua Urban, Daniel Boyle, and Yoonseong Park. (2016) Multiple functions of Na/K-ATPase in dopamine-induced salivation of the Blacklegged tick, Ixodes scapularis. Scientific Reports, Nature. 6:13-25. (peer reviewed)
Type: Journal Articles Status: Published Year Published: 2016 Citation: M. Djanaguiraman, D.L. Boyle, Ruth Welti, and P.V.V. Prasad. (2016) High temperature leads to leaf lipid changes, oxidation, physical damage, and decreased photosynthesis in wheat. Physiologia Plantarum. Submitted 12/21/2016. assigned the number PPL-2016-00437
Type: Journal Articles Status: Published Year Published: 2016 Citation: G. Gandhi, J. K. Amamcharla, and D. Boyle. (2016) Effect of milk protein concentrate (MPC80) quality on susceptibility to fouling during thermal processing. Journal of Dairy Science. Revised 12/18/2016.
Type: Journal Articles Status: Under Review Year Published: 2016 Citation: Brintha Girinathan, Marc Monot, Daniel Boyle, Kathleen McAllister, Joseph Sorg, Bruno Dupuy, and Revathi Govind "Effect of tcdR mutation on sporulation in the epidemic Clostridium difficile R20291 strain" American Society for Microbiology. Received December 22, 2016, under review.

Progress 10/01/14 to 09/30/15

Outputs
Target Audience:Main audience is Kansas State University faculty, graduate students, undergraduate students and researcher; also include Board of Reagents School, state K-12 schools, USDA, and businesses. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Number of individuals and labs given training or technical assistance: 1) faculty (44) 2) Graduate students and Postdocs (65) 3) Undergraduates (28) 4) Industry (6) 5) USDA (3) How have the results been disseminated to communities of interest?Publications- For further information on specific experiments, publications and grants, primary investigators will have to be consulted. What do you plan to do during the next reporting period to accomplish the goals?Continue with current strategy to accomplish goals.

Impacts
What was accomplished under these goals? Information and data obtained from this facility was incorporated into peer review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science. Clearly, this facility has been a valuable instrument for promoting the broad K-State goals of education, research, and service. The Kansas State University, Biology Microscopy Facility Contacts Phone (405) E-mail received (7,068) E-mail sent (888) Number of individuals and labs given training or technical assistance: faculty (44) Graduate students and Postdocs (65) Undergraduates (28) Industry (6) USDA (3) g) Departments and others using facility or services: The Microscopy Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the Kansas University, Fort Hays State University, USDA (Manhattan), American Oil Chemists Society (AOCS) and businesses such as Cargill, Nestle, ICM Inc. Saint Joseph, MO, SPX Cooling Technologies, and Agritrade International Inc., Deer Park, WA. Promoting the broad K-State goals of education, research, and service. Promoting goals of education, research, and service to the great K-State Community and businesses.

Publications

Type: Journal Articles Status: Published Year Published: 2015 Citation: Donghun Kim, Joshua Urban, Daniel Boyle, and Yoonseong Park. Multiple functions of Na/K-ATPase in dopamine-induced salivation of the Blacklegged tick, Ixodes scapularis. Scientific Reports, Nature. [Paper #SREP-15-28950] (accepted) (Peer reviewed).
Type: Journal Articles Status: Published Year Published: 2015 Citation: S. Narayanan, P. V. V. Prasad, A. K. Fritz, D. L. Boyle & B. S. Gill. HEAT STRESS Impact of High Night-Time and High Daytime Temperature Stress on Winter Wheat. J. Agronomy & Crop Science. 201: 206-218, 2015. (peer reviewed).
Type: Journal Articles Status: Published Year Published: 2015 Citation: Joseph Msanne, Ming Chen, Kyle D. Luttgeharm, Amanda M. Bradley, Elizabeth S. Mays, Janet M. Paper, Daniel L. Boyle, Rebecca E. Cahoon, Kathrin Schrick, and Edgar B. Cahoon. Glucosylceramides are critical for cell-type differentiation and organogenesis, but not for cell viability in Arabidopsis. The Plant Journal. Vol 84, Issue 1: 188-201, 2015. (peer reviewed

Progress 10/01/13 to 09/30/14

Outputs
Target Audience: The target audiences for this facility are Kansas State University and Kansas Regency Universities undergraduate and graduate students, post-doctorates, and faculty. This facility is also delivers science based knowledge through formal class room instruction (Biol 888), laboratory instruction, practicum experiences, development of curriculum or innovative teaching methodologies, experimental learning opportunities, and outreach to K-12 student and teachers, and companies. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Information and data obtained from this facility was incorporated into peer review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science. Clearly, this facility has been a valuable instrument for promoting the broad K-State goals of education, research, and service. 1) The Kansas State University, Biology Microscopy Facility Contacts a) Phone (405) b) E-mail received (6,891) c) E-mail sent (1,540) d) Number of individuals and labs given training or technical assistance: i) faculty (41) ii) Graduate students and Postdocs (62) iii) Undergraduates (12) iv) Industry (5) v) USDA (3) g) Departments and others using facility or services: The Microscopy Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the Kansas University, Fort Hays State University, USDA (Manhattan), The EXCITE program, and businesses such as Cargill, Nestle and ICM Inc. Saint Joseph, MO

Publications

Type: Other Status: Under Review Year Published: 2014 Citation: Joshua K. Craver, Chad T. Miller, Kimberly A. Williams, Daniel L. Boyle. Characterization and Comparison of Lesions on Ornamental Sweetpotato Blackie, Tomato Maxifort, Interspecific Geranium Caliente Coral, and Bat-faced Cuphea Tiny Mice. J. AMER. SOC. HORT. SCI. 139(5):603615. 2014. (peer reviewed)

Progress 01/01/13 to 09/30/13

Outputs
Target Audience: The target audiences for this facility are Kansas State University and Kansas Regency Universities undergraduate and graduate students, post-doctorates, and faculty. This facility is also delivers science based knowledge through formal class room instruction (Biol 888), laboratory instruction, practicum experiences, development of curriculum or innovative teaching methodologies, experimental learning opportunities, and outreach to K-12 student and teachers, and companies. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Information and data obtained from this facility was incorporated into peer review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science. Clearly, this facility has been a valuable instrument for promoting the broad K-State goals of education, research, and service. The Biology TEM Facility accomplished its goal through Phone (455) E-mail received (3302) E-mail sent (1604) Number of individuals and labs given training or technical assistance: faculty (41) Graduate students and Postdocs (66) Undergraduates (7) Industry (4) USDA (3) g) Departments and others using facility or services: The Microscopy Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), the GROW program, The EXCITE program, and businesses such as Cargill, Nestle and Nanoscale Materials Inc.

Publications

Progress 01/01/12 to 12/31/12

Outputs
OUTPUTS: The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University undergraduate and graduate students, post-doctoral research fellows, researchers and faculty from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), the GROW and EXCITE programs, K-12 students from USD 383 Manhattan/Ogden, and Junction City Schools, and researchers and extension agents from the Great Plains Diagnostic Network and Kansas businesses such as Cargill, and Nanoscale Materials Inc. From January 1 until December 6, 2012, a total of 309.7 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 38.9 hours of electron beam time generated during the servicing of this microscope. In addition to viewing samples on the TEM, approximate 929.1 hours of sample preparation time, consultation on projects, and grant support were supplied by this facility. Information and data obtained from this facility was incorporated into peer reviewed publications, extramural funding report updates and new extramural funding applications to NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted. PARTICIPANTS: In 2012, the Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University undergraduate and graduate students, post-doctoral research fellows, researchers and faculty from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), the GROW and EXCITE programs, K-12 students from USD 383 Manhattan/Ogden, and Junction City Schools, and researchers and extension agents from the Great Plains Diagnostic Network and Kansas businesses such as Cargill, and Nanoscale Materials Inc. TARGET AUDIENCES: The target audiences for this facility are Kansas State University and Kansas Regency Universities undergraduate and graduate students, post-doctorates, and faculty. This facility is also delivers science based knowledge through formal class room instruction (Biol 888), laboratory instruction, practicum experiences, development of curriculum or innovative teaching methodologies, experimental learning opportunities, and outreach to K-12 student and teachers, and companies. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Information and data obtained from this facility was incorporated into peer review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science. Clearly, this facility has been a valuable instrument for promoting the broad K-State goals of education, research, and service.

Publications

No publications reported this period

Progress 01/01/11 to 12/31/11

Outputs
OUTPUTS: The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University students (61) and researchers (38 faculty) from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), K-12 students from the GROW program, Manhattan High School, and Junction City High School, and researchers and extension agents from the Great Plains Diagnostic Network, the Naval Medical Research Center, and Kansas businesses such as Cargill, and Nanoscale Materials Inc. From January 1 until November 30, 2011, a total of 262.6 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 33.0 hours of electron beam time generated during the servicing of this microscope. In addition to viewing samples on the TEM, approximate 787.8 hours of sample preparation time, consultation on projects, and grant support were supplied by this facility. Information and data obtained from this facility was incorporated into peer reviewed publications, extramural funding report updates and new extramural funding applications to NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted. PARTICIPANTS: In 2011, the Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University students (61) and researchers (38 faculty) from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), the GROW program, Manhattan High School, and Junction City High School, and researchers and extension agents from the Great Plains Diagnostic Network, the Naval Medical Research Center, and Kansas businesses such as Cargill, and Nanoscale Materials Inc. TARGET AUDIENCES: The target audiences for this facility are Kansas State University and Kansas Regency Universities undergraduate and graduate students, post-doctorates, and faculty. This facility is also delivers science based knowledge through formal class room instruction, laboratory instruction, practicum experiences, development of curriculum or innovative teaching methodologies, experimental learning opportunities, and outreach to K-12 student and teachers, and companies. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Information and data obtained from this facility was incorporated into peer review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science. Clearly, this facility has been a valuable instrument for promoting the broad K-State goals of education, research, and service

Publications

No publications reported this period

Progress 01/01/10 to 12/31/10

Outputs
OUTPUTS: The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), the GROW program, and Kansas businesses such as Cargill and Nanoscale Materials Inc. This included 37 faculty members, 10 postdoctoral fellows, 35 graduate students and 4 undergraduate students, and 15 individuals from the GROW program. From January 1 until December 10, 2010, a total of 339.7 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 58.0 hours of electron beam time generated during the servicing of this microscope. In addition to viewing samples on the TEM, approximate 1,019.1 hours of sample preparation time, consultation on projects, and grant support were done at this facility. Data generated at this facility was once again instrumental to researchers at KSU publishing results and obtaining extramural funding from NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted. PARTICIPANTS: In 2010, the Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), the GROW program, and Kansas businesses such as Cargill, and Nanoscale Materials Inc. TARGET AUDIENCES: The target audiences for this facility are Kansas State University and Kansas Regency Universities undergraduate and graduate students, post-doctorates, and faculty. This facility is also used to deliver science based knowledge through formal class room instruction, laboratory instruction, practicum experiences, development of curriculum or innovative teaching methodologies, experimental learning opportunities, and outreach to K-12 student and teachers, and companies. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Information and data obtained from this facility was incorporated into pier review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science.

Publications

No publications reported this period

Progress 01/01/09 to 12/31/09

Outputs
OUTPUTS: The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), Fort Hays State University, the GROW program, and Kansas businesses such as Cargill, Nanoscale Materials Inc., and Edenspace Systems Corp. This included 41 faculty members, 18 postdoctoral fellows, 31 graduate students and 5 undergraduate students, and 12 individuals from the GROW program. From January 1 until December 14, 2009, a total of 328.6 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 60.0 hours of electron beam time generated during the servicing of this microscope. In addition to viewing samples on the TEM, approximate 985.8 hours of sample preparation time, consultation on projects, and grant support were done at this facility. Data generated at this facility was once again instrumental to researchers at KSU publishing results and obtaining extramural funding from NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted. PARTICIPANTS: In 2009, the Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), Fort Hays State University, the GROW program, and Kansas businesses such as Cargill, Nanoscale Materials Inc., and Edenspace Systems Corp. TARGET AUDIENCES: Kansas State University and Kansas Regency Universities undergraduate and graduate students, post-doctorates, and faculty. This facility is also used to deliver science based knowledge through formal class room instruction, laboratory instruction, practicum experiences, development of curriculum or innovative teaching methodologies, experimental learning opportunities, and outreach to K-12 student and teachers, and companies. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Information and data obtained from this facility was incorporated into pier review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science.

Publications

No publications reported this period

Progress 01/01/08 to 12/31/08

Outputs
OUTPUTS: The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), Fort Hays State University, the GROW program, and local businesses such as Nanoscale Materials Inc. This included 38 faculty members, 21 postdoctoral fellows, 36 graduate students and 4 undergraduate students, and 12 individuals from the GROW program. From January 1 until November 30, 2008, a total of 209.5 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 22.4 hours of electron beam time generated during the servicing of this microscope. In addition to viewing samples on the TEM, approximate 838 hours of sample preparation time, consultation on projects, and grant support were done at this facility. Data generated at this facility was once again instrumental to researchers at KSU publishing results and obtaining extramural funding from NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: In 2008, the Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), Fort Hays State University, the GROW program, and local businesses such as Nanoscale Materials Inc. The target audiences for this facility are Kansas State University and Kansas Regency Universities undergraduate and graduate students, post-doctorates, and faculty. This facility is also used to deliver science based knowledge through formal class room instruction, laboratory instruction, practicum experiences, development of curriculum or innovative teaching methodologies, experimental learning opportunities, and outreach to K-12 student and teaches, and companies. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Information and data obtained from this facility was incorporated into pier review publications, extramural funding report updates and new extramural funding applications. This facility also gave K-12 school aged children scientific knowledge, scientific skills, and encouragement to pursue careers in science.

Publications

No publications reported this period

Progress 01/01/07 to 12/31/07

Outputs
The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), Midwest Research Institute, the GROW program, and local businesses such as Nanoscale Materials Inc. This included 53 faculty members, 21 postdoctoral fellows, 34 graduate students and 3 undergraduate students, and 12 individuals from the GROW program. From January 1 until November 30, 2007, a total of 166.1 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 2.4 hours of electron beam time generated during the servicing of this microscope. In addition to viewing samples on the TEM, approximate 664.4 hours of sample preparation time, consultation on projects, and grant support were done at this facility. Data generated at this facility was once again instrumental to researchers at KSU publishing results and obtaining extramural funding from NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted.

Impacts
Information and data obtained from this facility was incorporated into pier review publications, extramural funding report updates and new extramural funding applications.

Publications

No publications reported this period

Progress 01/01/06 to 12/31/06

Outputs
The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the USDA (Manhattan), Fort Hayes State University, Graceland University (Lamoni, IA), the GROW program, and local businesses such as Nanoscale Materials Inc. This included 39 faculty members, 17 postdoctoral fellows, 31 graduate students and 11 undergraduate students, and 12 individuals from the GROW program. From January 1 until November 30, 2006, a total of 337.1 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 121.5 hours of electron beam time generated during the servicing of this microscope. In addition, approximate 674.2 hours of sample preparation time and consultation on projects were done at this facility prior to viewing samples on the TEM. Data generated at this facility was once again instrumental to researchers at KSU publishing results and obtaining extramural funding from NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted

Impacts
Information and data obtained from this facility was incorporated into pier review publications, extramural funding report updates and new extramural funding applications. In addition, this facility was instrumental in the identification and characterization of a new virus infecting this year's wheat crop.

Publications

No publications reported this period

Progress 01/01/05 to 12/31/05

Outputs
The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the GROW program, and local businesses such as Nanoscale Materials Inc. This included 34 faculty members, 14 postdoctoral fellows, 25 graduate students and 5 undergraduate students, and 24 individuals from the GROW program. From January 1 until November 28, 2005, a total of 167.4 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 39.3 hours of electron beam time generated during the servicing of this microscope. In addition, approximate 503 hours of sample preparation time and consultation on projects were done at this facility prior to viewing samples on the TEM. Data generated at this facility was once again instrumental to researchers at KSU publishing results and obtaining extramural funding from NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted.

Impacts
Information and data obtained from this facility was incorporated into pier review publications, extramural funding report updates and new extramural funding applications

Publications

No publications reported this period

Progress 01/01/04 to 12/31/04

Outputs
The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as the Agricultural Research Center-Hays, the GROW program, Kansas 7-12 schools and local businesses such as Nanoscale Materials Inc. This included 23 faculty members, 10 postdoctoral fellows, 24 graduate students and 5 undergraduate students, 28 individuals from the GROW program and over 100 visitors from grades 7 through 12. From January 1 until November 19, 2004, a total of 188 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 4.9 hours of electron beam time generated during the servicing of this microscope. In addition, approximate 480 hours of sample preparation time was done at this facility prior to viewing samples on the TEM. Data generated at this facility was once again instrumental to researchers at KSU publishing results and obtaining extramural funding from NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted.

Impacts
Information and data obtained from this facility was incorporated into pier review publications, extramural funding report updates and new extramural funding applications.

Publications

No publications reported this period

Progress 01/01/03 to 12/31/03

Outputs
The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as local businesses such as Nanoscale Materials Inc. This included 15 faculty members, 4 postdoctoral fellows, 22 graduate students and one undergraduate student. From January 1 until November 25, 2003, a total of 247.4 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the 46.2 hours of electron beam time generated during the servicing of this microscope. In addition, approximate 990 hours of sample preparation time was done at this facility prior to viewing samples on the TEM. Data generated at this facility was once again instrumental to researchers at KSU publishing results and obtaining extramural funding from NIH, Department of Defense, USDA and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted.

Impacts
Information and data obtained from this facility was incorporated into pier review publications, extramural funding report updates and new extramural funding applications.

Publications

No publications reported this period

Progress 01/01/02 to 12/31/02

Outputs
The Biology Transmission Electron Microscopy (TEM) Facility was used by Kansas State University researchers from the colleges of Agriculture, Arts and Sciences, Engineering, Human Ecology and Veterinary Medicine as well as local businesses such as Nanoscale Materials Inc. This included 10 faculty members, 9 postdoctoral fellows, 17 graduate students and two undergraduate students. From January 1 until November 25, 2002, a total of 271.9 hours of electron beam time have been logged on the FEI CM100 TEM. This does not include the approximate 1088 hours of sample preparation time done at this facility prior to viewing samples on the TEM. Data generated at this facility was once again instrumental to researchers at KSU publishing results and obtaining extramural funding from NIH, Department of Defense, and NSF. For further information on specific experiments, publications and grants, primary investigators will have to be consulted. In addition, Dr. Boyle offered a Topics in Biology 890 class in the fall of 2002, that was taken for credit by 7 graduate students and audited by 2 graduate students and 2 postdoctoral fellows. This was a 3 credit class with objectives to familiarize students with theories of TEM, understanding experimental design, operation of the TEM and developing procedures and protocols for there own research projects.

Impacts
Information and data obtained from this facility was incorporated into pier review publications, extramural funding report updates and new extramural funding applications. For an example, refer to the cover image of Langmiur, August 20, 2002 (http://pubs3.acs.org/acs/journals/cover_art.page?incoden=langd5#) taken from this facility.

Publications

No publications reported this period

Progress 01/01/01 to 12/31/01

Outputs
The Biology Research Microscope and Imaging Facility was again used campus wide in 2001. From January 1 to November 30, a total of 420 beam hours were logged on the 2 Philips TEMS by 19 faculty from 9 departments and their graduate students (17) and postdoctoral associates(5). This equals to an ON/OFF complement of 1050 hours. Teaching and class demonstrations used 67 hours on the EM 201. For research, 324 hours were logged on both the 201 and CM100. Scope servicing used 28.4 hours on both. Investigators worked on: brine shrimp development at 0 and 1g; insect viruses - and gene-induced apoptosis in cultured cells; proteoglycan gene effects on mouse collagen; cataractogenesis; meat processing and bacterial contamination; immunogold methods to localize the proteoglycan gene and to see if the cell regulator CeReS-18 affects polyoma virus replication; metallic nanoparticle sizing and lattice formation; and metal oxides in bacterial decontamination. Many studies will continue in 2002. Drs. Dan Boyle and Avelina Paulsen provided assistance. Primary investigators will have to be consulted for publications and results.

Impacts
Most information obtained were basic structure, function or diagnostic data, but users are looking into practical applications of their results.

Publications

No publications reported this period

Progress 01/01/00 to 12/30/00

Outputs
Both Philips TEMs were used from January 1-October 31, 2000 by 5 postgraduate scientists and 37 graduate students working with 23 faculty from 7 KSU departments. A total of 356 hr was logged on both Philips CM100 and EM201, with an ON/OFF equivalent time of 890.0 hr. Projected usage from Nov 1 - Dec 31 are 40-60 hours on both scopes. The CM100 was used strictly for research (108.0 hr) with 8.4 hr used for scope servicing. On the EM201 69.5 hr was used for teaching and scope servicing, and 80.1 hr for research. The facility was also used for preparing TEM samples and for photographic and graphic imaging techniques. Major projects, some already finished but many are being continued include: brine shrimp development; polyoma virus apoptotic effects and viral replication, Cryptosporidium virus structure and localization in hosts (Biol.); milk fat globule structure as affected by processing and aging, and the effects of NaCl on 4 food-borne bacterial spp (An.Sci.); rice and Arabidopsis phospholipases by immunogold localization (Pl. Path, Biochem); carbon and gold colloid formation (Physics); and various metalloorganic nanoparticles in gel or powder form that may be useful as defense vs biological agents (Chem., Chem Engg, Nantek). For information on these, primary investigators (and their publications) should be contacted. Dan Boyle and Avelina Paulsen managed and provided assistance.

Impacts
Structure and function as seen by high resolution techniques, either cytochemically (biology) or by higher magnification and diffraction (chemistry, physics), were the major objects of study undertaken on the TEMS this year. Many of these studies are continuing into 2001.

Publications

No publications reported this period

Progress 01/01/99 to 12/31/99

Outputs
Both Philips TEMs were actively used in 1999 by 11 post graduate scientists and 57 graduate students working with 35 faculty from 9 KSU departments. A total of 568 beam hr was logged on both scopes, with an equivalent ON/OFF time of 1420 hr. On the EM 201 62.4 hr was used for teaching, demonstrations and servicing, and 168 hr on research. The CM100 was used strictly for research with 305.4 hr logged plus 8.4 hr for scope maintenance. Two Physics REU undergraduates (non KSU) were helped with their research on the EM201. The facility was used by many for preparing samples and for photographic and other graphic imaging technics. Brine shrimp and plant development under microgravity, snail egg cytokinesis, viral proteins, bread dough, as well as work on metalloorganic production and characterization were continued. New research included viral apoptosis in various cell systems; immunolocalization with gold of viral proteins, plant phospholipases in genetically transformed Arabidopsis and bacterial rice spot leaves; detoxification of food bacterial pathogens; characterization by electron diffraction of nanoparticles of metal oxides; possible use of certain organic nanoparticles as bacterial killers; and viewing of particles collected on microfilters for air pollution control. For information on these, primary investigators (and their publications) will have to be contacted.

Impacts
The variety of research projects done in the facility continues to grow particularly as the facility becomes more involved with the safety concerns in both food and environment. Some researchers and students now doing confocal laser scanning microscopy eventually plan to correlate their research with EM investigations.

Publications

No publications reported this period

Progress 01/01/98 to 12/31/98

Outputs
Both Philips TEMs were used in 1998 for a total beam hr. time of 417.3 which has an equivalent ON/OFF time of 1043.3 hr. including servicing and maintenance hours. Beam hr. usage on the EM 201 were 86 hr for teaching and class demonstrations, 103.7 hr. for research; on the CM 100, 188.6 hr. was used for research. For research and teaching the facility was used by 7 post-doctorates and 20 graduate students working with 25 KSU faculty members in Biology (13), Anatomy and Physiology (1), Animal Science (2), Biochemistry (2) Chemistry (2), Grain Science (1), Horticulture (1), Physics (2), and Plant Pathology (2), Two non KSU users were helped: Nantek, Inc. (Manhattan, KS), and NASA Molecular and Life Support Group (Kennedy Space Center, FL). Biological samples included brine shrimp, bacteria, viruses, other disease-producing organisms, and agricultural products like cheese and bread dough. Ultrasmall metalloorganic compounds and films were also studied. Dr. Avelina Paulsen provided for instruction, viewing, photography and consultations; she was joined later in the year by Dr. Dan Boyle in running the facility.

Impacts
(N/A)

Publications

No publications reported this period

Progress 01/01/97 to 12/31/97

Outputs
In the Division of Biology, 2 TEMs are now available for campus and noncampus users by contacting the Biology Research Microscope and Image Processing Facility. A Philips CM100 with cryoEM capability was installed in June. It was purchased with KS NASA EPSCOR grant funds to Dr. J. Guikema, P.I., and matching support from the state of KS and KSU. In 1997, the scopes were used for a total of 345.4 beam hr. (211.4 hr research and 134 hr for teaching and scope maintenance), with an ON/Off scope time equivalence of 864 hr. For research and teaching, the scopes were used by 18 faculty in Biology (11), Biochemistry (1), Foods and Nutrition (2), Physics (1), and Plant Pathology (3) and by 6 postdoctorates and 24 graduate students working with them. Ultrastructural research on the development of various structures in mammals, quail, shrimp, several microorganisms and plants; diagnosis of plant pathogens, as well as structures of metaloorganic compounds and biodegradable plastics were done. Three campus-wide training sessions were also conducted on the CM100 to acquaint potential users with its capabilities. Dr. Avelina Paulsen runs the Facility and provided for instruction, assistance in sample preparation, viewing, photography and interpretation of structures observed.

Impacts
(N/A)

Publications

Publications will be reported by individual investigators.

Progress 01/01/96 to 12/30/96

Outputs
The Philips EM 201 electron microscope, a part of the Division of Biology Microscope Facility, was again central to several on-going researches by University faculty and their students. A total 361.2 beam hr. was used: 165 hr involved ultrastructural, developmental and immune cytochemical studies on biological chemical and metallic samples; and 95 hr. were used in teaching, demonstrations, and scope maintenance. This is equivalent to a scope "ON" time of 903.0 hr. Eighteen faculty, 6 post-graduate and 40 graduate students from Biology, Chemistry, Entomology, Foods and Nutrition, Physics and Plant Pathology used the microscope. Dr. Avelina Paulsen provided for instruction, sample preparation, viewing and photography.

Impacts
(N/A)

Publications

Publications will be reported by individual investigators.

Progress 01/01/95 to 12/30/95

Outputs
As part of Biology's Microscopy Laboratory, the transmission electron microscopewas used by 24 faculty members and their graduate students (42) for research and teaching purposes. One undergraduate summer intern in Physics also used the facility. The total logged beam time was 313 hr. with a complement of 626+ hours for research, servicing, teaching and demonstrations given to various high school and undergraduate students that requested the service. In addition to supervisory duties, Dr. Avelina Paulsen provided for instruction, sample preparation, viewing and photography on the Philips EM 201.

Impacts
(N/A)

Publications

Progress 01/01/94 to 12/30/94

Outputs
The TEM service facility was used by 23 faculty, 4 postgraduate and 37 graduate students from 9 KSU departments. One undergraduate summer intern in Physics also used the microscope. Of a total 383 hr. logged on the Philips EM201, 324 hr. (49%) was used for various research activities and the rest for classes and demonstrations. This represents a scope "ON" time of 766 hr. Dr. Avelina Q. Paulsen continues to manage the facility, and also provides for instruction, sample preparation, viewing, and photography.

Impacts
(N/A)

Publications

Progress 01/01/93 to 12/30/93

Outputs
In 1993 the TEM facility was used by 22 faculty, 5 post-graduate and 35 graduatestudents from 9 KSU departments. Of the total 576 hr logged on the Philips EM 201, 436 hr. were used in research and 140 hr. in teaching, demonstrations to high school science students and scope servicing. This is equivalent to a scope "ON" time of 1440 hr. Research was done by or for faculty and students in the Departments of Biology, Biochemistry, Chemistry, Physics, Animal Science and Industry, Entomology, Plant Pathology, Foods and Nutrition and Horticulture. The facility is run single-handedly by Dr. Avelina Q. Paulsen. She provides not only for instruction and scope maintenance, sample preparation, and photography, but also is available for consultations on techniques and analysis of results. In addition she teaches the course Biology 888A which deals with TEM techniques every spring semester. A summary of beam usage by various departments is attached.

Impacts
(N/A)

Publications

Progress 01/01/92 to 12/30/92

Outputs
As in previous years, the transmission electron microscope (TEM) facility was central to various research activities across the university. The total logged time was 537.0 hr. with a complement of 1075.0 hr. of "on time" for research, education, extension, and scope maintenance. Scientific research in the facility, was done by or for faculty, postgraduate and graduate students from the Division of Biology, the Departments of Agronomy, Animal Science and Industry, Chemistry, Physics, Plant Pathology, and Veterinary Medicine. Students from several other departments are presently preparing samples for viewing in the Philips EM 201 electron microscope. The TEM facility continues to be run smoothly, efficiently and single-handedly by Dr. Avelina Paulsen. She also provides for servicing of instrumentation, and in many of these research studies, prepares, views and photographs the samples, or provides instruction for those that want to operate the instrument themselves.

Impacts
(N/A)

Publications

Progress 01/01/91 to 12/30/91

Outputs
During 1990 the transmission electron microscope (TEM) facility once again increased its central role in the research activities of numerous scientists and graduate students across the campus. The total logged beam time was 342 hours with a complement of 785+ hours of "on time" for research, service and education. During the year individuals used the TEM facility to support their scientific research programs. This included faculty scientists and graduate students from the Division of Biology, the Departments of Agronomy, Animal Sciences and Industry, Chemistry, Entomology, Physics, Plant Pathology, Foods and Nutrition in the College of Human Ecology, and investigators from the College of Veterinary Medicine. Further application involved the hands on training of graduate students, one visiting scientist in Agronomy, and several classes held here at K-State. The TEM has continued to receive outstanding care by Dr. Avelina Paulsen who provides assurance that the facility is available to all research scientists, and runs the operation in a smooth and efficient manner. In addition to overseeing the TEM facility, Dr. Paulsen is responsible for service requirements, sample preparation, and instruction in the operation of the instrument.

Impacts
(N/A)

Publications

Progress 01/01/90 to 12/30/90

Outputs
During 1990 the transmission electron microscope (TEM) facility once again increased its central role in the research activities of numerous scientists and graduate students across the campus. The total logged beam time was 281 hours with a complement of 613+ hours of "on time" for research, service and education. During the year 40 individuals used the TEM facility to support their scientific research programs. This included faculty scientists and graduate students from the Division of Biology, the Departments of Agronomy, Entomology, Physics, Plant Pathology, Foods and Nutrition in the College of Human Ecology, and investigators from the College of Veterinary Medicine. Further application involved the Department of Microbiology at the University of Kansas and several classes held here at K-State. The TEM has continued to receive outstanding care by Dr. Avelina Paulsen who provides assurance that the facility is available to all research scientists, and runs the operation in a smooth and efficient manner. In addition to overseeing the TEM facility, Dr. Paulsen is responsible for service requirements, sample preparation, and instruction in the operation of the instrument.

Impacts
(N/A)

Publications

Progress 01/01/89 to 12/30/89

Outputs
During 1989, the transmission electron microscope (TEM) facility maintained, andactually significantly increased, its central role in the research of numerous scientists and graduate students across the university. The total logged beam time was 271 hours with an equivalent of 572 hours of "on time" for research, service, and education. During the year 41 individuals used the TEM facility to support their scientific research programs. This included faculty scientists and graduate students of the Division of Biology, the Departments of Agronomy, Entomology, Horticulture, Plant Pathology, Physics, as well as Foods and Nutrition in the College of Human Ecology, and representatives in the College of Veterinary Medicine. In addition to serving campus research programs, the TEM facility augmented scientific research in the Department of Microbiology at the University of Kansas and educational programs, for both on-campus students and faculty and students from other universities attending research workshops here at K-State. Dr. Avelina Paulsen has continued to do an outstanding job in maintaining this facility and assuring an efficient and smooth operation. In addition to routine TEM operation, she assumes responsibility for service requirements, sample preparation, and instruction in the operation of the instrument.

Impacts
(N/A)

Publications

Progress 01/01/88 to 12/30/88

Outputs
The transmission electron microscope (TEM) facility has continued to be an important resource for numerous research investigators at Kansas State University. During 1988 the total logged beam time was 232 hours with an equivalent of 464 hours on "on time" for research, classes, and service. During the last year 52 individuals used the TAM facility to support their research programs. This included faculty scientists, research associates, and graduate students of the Division of Biology and the Departments of Agronomy, Grain Science, Horticulture, Plant Pathology, Foods and Nutrition, and representatives of the College of Veterinary Medicine. In addition to on campus research programs, the TEM facility was used to augment science and educational activities of Agriservice Assoc. of Manhattan, KS, Colby High School, Colby, KS, and the University of the West Indies, Trinidad. Dr. Avelina Paulsen does an outstanding job of caring for this facility and assuring a smooth and efficient operation. In addition to routine TEM operation, she assumes responsibility for service requirements, sample preparation, and instruction for undergraduate and graduate students.

Impacts
(N/A)

Publications

Progress 01/01/87 to 12/30/87

Outputs
During the past year the Transmission Electron Microscope facility served numerous scientific investigators and played a key role in the progress of many research projects. The total logged beam time during the last year was 178 hours with an equivalent of 398 hours of "on" time for research, classes, and service. During the past year 59 individuals used the Transmission Electron Microscope facility in their research work. This included faculty scientists and graduate students of the Division of Biology and the Departments of Agronomy, Entomology, Horticulture, Foods and Nutrition, Plant Pathology, as well as researchers in the College of Veterinary Medicine. In addition to scientists on campus, the use of the Transmission Electron Microscope facility served research programs at the Ahmadu Bello University at Nigeria, Agriservice Assoc. of Manhattan, and the University of the West Indies at Trinidad. Dr. Avelina Paulsen provided excellent consultation services, oversees the operation and maintenance of this important facility, and teaches a graduate course in the application of electron microscopy in biological and agricultural research.

Impacts
(N/A)

Publications

Progress 01/01/86 to 12/30/86

Outputs
During the past year the transmission electron microscope facility served numerous investigators and played an important role in the progress of many research projects. The total logged beam time during the last year was 234 hours with an equivalent of 467 hours of "on" time for research, classes, and service. During the year over 40 individuals used the electron microscope in their research programs. This included faculty scientists, research associates, and graduate students of the Division of Biology and the Departments of Agronomy, Animal Sciences and Industry, Entomology, Horticulture, Physics, and Plant Pathology. In addition, the use of the TEM facility supported a research program initiated in Ahmadu Bello University Nigeria and service for the Kansas Department of Transportation. Dr. Avelina Paulsen oversees this research facility and provides excellent consultation services, carefully maintains the TEM in operational order, and teaches a graduate course in the application of electron microscope in biological and agricultural research.

Impacts
(N/A)

Publications

Progress 01/01/85 to 12/30/85

Outputs
The transmission electron microscopy (TEM) facility continued to be a valuable resource for research scientists during 1985. The total logged beam time during the last year was 348 hours. The TEM actually was "on" 687 hours for research, instruction and maintenance. This year over 70 individuals used the TEM facility in their research projects. This included faculty scientists, postdoctoral fellows, and graduate students of the College of Agriculture, College of Arts and Sciences, and the College of Veterinary Medicine, representing the Division of Biology, and the Departments of Agronomy, Biochemistry, Entomology, Foods and Nutrition, Plant Pathology, and Anatomy and Physiology. In addition, the TEM facility was used in collaborative research projects with the Veterinary Anatomy and Medicine Hospital of Ahmadu Bello University in Nigeria, the Plant Pathology Section, USDA-ARS of Bushland, Texas, and the Microbiol. Sec., Department of Transportation, Kansas. Dr. Avelina Paulsen continued her valuable efforts in maintaining the TEM facility in excellent working condition, in providing invaluable consultation to scientists, and presenting a graduate course in the use of the TEM facility.

Impacts
(N/A)

Publications

Progress 01/01/84 to 12/30/84

Outputs
The transmission electron microscopy (TEM) facility continued to be a key research resource for AES scientists during 1984. The total beam time logged-in last calendar year was 370 hours. The TEM actually was "on" 740 hours for research, maintenance, and instruction during 1984. Sixty individuals used the TEM facility during the past year. This included faculty scientists, research associates, and graduate students from the Division of Biology, the Departments of Agronomy, Entomology, Horticulture, Plant Pathology, and the College of Veterinary Medicine. In addition, research projects have been aided for scientists at Wichita State University, Ahmadu Bello University in Nigeria, the USDA team at Bushland, TX, and at Columbia, MO. In most of these latter projects all sample processing, resin embedment, sectioning, microscopy, and photographic enlarging was carried out by Dr. Avelina Paulsen. As always, Dr. Paulsen has continued her excellent work in maintaining the TEM facility, providing invaluable consultation, and providing a graduate course in the use of the facility.

Impacts
(N/A)

Publications

Progress 01/01/83 to 12/30/83

Outputs
The transmission electron microscopy (TEM) facility continued to be an importantresource for our research scientists during 1983. The total beam time logged-in during the last year was 395 hours. The TEM was actually "on" 790 hours for research, instruction, and maintenance. Thirty-one individuals used the TEM facility during the past year. This includes faculty scientists, research associates, and graduate students forom the Division of Biology, the Departments of Plant Pathology, Agronomy, Chemical Engineering and individuals from Fort Hays State University. Dr. Avelina Paulsen has continued her excellent work in maintaining the TEM facility, providing invaluable consultation, and presenting a graudate course in the use of the facility.

Impacts
(N/A)

Publications

Progress 01/01/82 to 12/30/82

Outputs
During the 1982 year the Transmission Electron Microscope (TEM) facility continued to serve as a primary research facility for programs across the university campus. The Philips 201 TEM logged over 440 hours of beam-on time. Twenty-five faculty members used the TEM facility and they represented the Departments of Agronomy, Biochemistry, Plant Pathology, Entomology, the Division of Biology, and members of the College of Veterinary Medicine. In addition, 22 graduate students and postdoctoral fellows made use of the research facility for their scientific programs. The TEM facility has the excellent professional services of Dr. Avelina Paulsen who oversees the Philips 201 TEM and its maintenance, preparation facilities, and photo-reproduction rooms. In addition to her consultation with faculty and student scientists, she teaches the use of the instrument and demonstrates the use of TEM for classes across campus. Publications are reported by the primary investigators of these projects.

Impacts
(N/A)

Publications

Progress 01/01/81 to 12/30/81

Outputs
During 1981 the Transmission Electron Microscope (TEM) facility continued to provide a valuable resource to ongoing research programs across our campus. The Philips 201 TEM logged over 830 hours of beam-on time furing the last calendar year and this represents over a 100% increase compared to the previous year. During 1981--41 faculty members used this TEM facility and they represented the Division of Biology; and the Department of Agronomy, Biochemistry, Grain Science, Entomology, Nuclear Engineering, Plant Pathology, Horticulture, Foods and Nutrition, and individuals in the College of Veterinary Medicine. In addition, 32 graduate students and postdoctoral fellows made use of this facility in their research projects. Students and postdoctoral fellows represented a wide range of departments as reported above for faculty scientists. The TEM facility has the excellent professional services of Dr. Avelina Paulsen who oversees the Philips 201 TEM, preparation facilities, and photo-reproduction rooms. Most importnatly, he offers first-rate consultation and she has worked with numerous faculty, post-doctoral, and student scientists. Publications are reported by the primary investigators of these projects.

Impacts
(N/A)

Publications

Progress 01/01/80 to 12/30/80

Outputs
During 1980 the Transmission Electron Microscope (TEM) facility continued to be important to numerous ongoing research projects on our campus. The Philips 201 TEM logged over 407 hours of beam-on time during the last calendar year and this represents over a 20% increase compared to the previous year. The number of faculty members using this facility during 1980 numbered 27. They represented the Division of Biology; and the Departments of Agronomy, Biochemistry, Grain Science, Nuclear Engineering, Plant Pathology, and the College of Veterinary Medicine. In addition, the facility supported the research of 19 graduate students and postdoctoral fellows from across the university (Agronomy, Biochemistry, Biology, Grain Science, Nuclear Engineering, Plant Pathology and Veterinary Medicine). The TEM facility has the excellent consultant services of Dr. Avelina Paulsen who also maintains the TEM, preparation facilities and photo-reproduction rooms. She has worked with the numerous faculty members and students who have sought out her advice and aid in their research programs and taught a graduate level course in electron microscopy. Publications would be reported by the primary investigators of these projects.

Impacts
(N/A)

Publications

Progress 01/01/79 to 12/30/79

Outputs
During the 1979 calendar year the TEM facility was a central and critical support unit to numerous scientists who required the use of biological transmission electron microscopy in their research programs. The Philips 201 transmission electron microscope logged over 337 hours on beam-on time during the last year. The number of faculty members using this facility increased to 31 in 1979. These faculty members represented the Division of Biology and the Departments of Biochemistry, Agronomy, Grain Science, Plant Pathology, Entomology, Anatomy and Physiology, Surgery and Medicine, Pathology, Animal Science, and the Veterinary Clinical Services. In addition, we have identified 26 graduate students and postdoctoral fellows who have used this facility during the last year. The TEM facility activities have included the valuable services of Dr. Avelina Paulsen, who is a half-time instructor and served as a technical consultant to the faculty, students, and postdoctoral fellows who used the TEM in the research programs.

Impacts
(N/A)

Publications

Progress 01/01/78 to 12/30/78

Outputs
The TEM facility has continued to serve a vital service for numerous investigators who use biological transmission electron microscopy in the research program. During the calendar year of 1978, the Philips 201 transmission electron microscope logged over 554 hours of beam-on time. The number of faculty who used the facility increased to 29 in 1978. These faculty members represented the Division of Biology and the Departments of Plant Pathology, Entomology, Grain Science, Animal Science, Agronomy, Biochemistry, Industrial Engineering, Anatomy and Physiology, Pathology, Infectious Diseases and the Veterinary Diagnostic Laboratory. In addition, we have identified 23 graduate and post-graduate trainees who have used this facility during 1978 to aid their research projects. The TEM facility activities have included the valuable services of Dr. Avelina Paulsen who is a half-time instructor in the facility.

Impacts
(N/A)

Publications

Progress 01/01/77 to 12/30/77

Outputs
This TEM facility continues to service the entire campus for biological transmission electron microscopy. In the calendar year of 1977, the Philips 201 transmission electron miscroscope logged 520 hours of beam-on time. This represents approximately 20 hours/week of scheduled use. The number of faculty who used the facility has increased to 26 in 1977. These faculty represent the Division of Biology and the Department of Pathology, Infectious Disease, Biochemistry, Nuclear Engineering, Chemical Engineering, Entomology, Animal Science, Grain Science and Dykstra Veterinary Hospital. In addition, we have identified 36 graduate students and 3 research associates who have used this facility during 1977 to aid their research projects.

Impacts
(N/A)

Publications

Progress 01/01/76 to 12/30/76

Outputs
This TEM facility services the entire campus for biological transmission electron microscopy. In calendar year 1976, the Philips 201 transmission electron microscope logged 560 hours of beam-on time. This total would amount to approximately 20 hours/week of scheduled use. The number of faculty using the facility increased from 12 in 1975 to 19 in 1976, representing the Division of Biology, and the Departments of Veterinary Pathology, Infectious Diseases, Plant Pathology, and Entomology. Facility use included the services of Dr. Avelina Paulsen who is a half-time instructor in the facility.

Impacts
(N/A)

Publications

Progress 01/01/75 to 12/30/75

Outputs
This TEM facility services the entire campus for biological transmission electron microscopy. In calendar year 1975, the instrument logged 597 hours of beam-on time; this slightly increased use would amount to over 20 hours/wk of scheduled use. Twelve faculty members in the Division of Biology, and the Departments of Veterinary Pathology, Infectious Diseases, and Plant Pathology used the facility, including the services of Dr. Avelina Paulson who is a half-time instructor in the facility.

Impacts
(N/A)

Publications