Source: UNIV OF MINNESOTA submitted to NRP
FACTORS AFFECTING FOOD PRECURSORS, FOOD QUALITY AND METHODS OF ASSESSMENT - COOPERATIVE ELECTRON OPTICS FACILITY/IMAGING CENTER
Sponsoring Institution
State Agricultural Experiment Station
Project Status
COMPLETE
Funding Source
STATE
Reporting Frequency
Annual
Accession No.
0032220
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2011
Project End Date
Jun 30, 2016
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIV OF MINNESOTA
(N/A)
ST PAUL,MN 55108
Performing Department
College of Biological Sciences
Non Technical Summary
The UIC supports basic and multi-disciplinary applied research through microscopic imaging technologies aimed at evaluating options for improving animal health and food safety. This project revision continues MAES support funding, and ensures researchers access to state-of-the-art equipment and staff in these research efforts. There have been over 2500 research and instructional posters printed. Past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life- Fungus) relied on our facility. There were multi-day workshops that were sponsored by the Centers that trained 30+ users on pioneering technical methodologies in sample preparation. Tours to dozens of K-12 students and teachers were well received. Exhibits at the Minnesota Zoo and the Science Museum of Minnesota are based on results from staff and collaborations.
Animal Health Component
17%
Research Effort Categories
Basic
73%
Applied
17%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2011545104010%
2062499103010%
5013430200010%
5023520103010%
2124010103010%
2144030103010%
1014050104010%
7013430200010%
7125010116010%
7117299103010%
Knowledge Area
212 - Pathogens and Nematodes Affecting Plants; 206 - Basic Plant Biology; 214 - Vertebrates, Mollusks, and Other Pests Affecting Plants; 711 - Ensure Food Products Free of Harmful Chemicals, Including Residues from Agricultural and Other Sources; 501 - New and Improved Food Processing Technologies; 712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins; 101 - Appraisal of Soil Resources; 201 - Plant Genome, Genetics, and Genetic Mechanisms; 502 - New and Improved Food Products; 701 - Nutrient Composition of Food;

Subject Of Investigation
3430 - Cheese; 4050 - Protozoa; 3520 - Meat, swine; 4010 - Bacteria; 4030 - Viruses; 7299 - Research equipment and methods, general/other; 1545 - Durum wheat; 5010 - Food; 2499 - Plant research, general;

Field Of Science
1040 - Molecular biology; 1030 - Cellular biology; 1160 - Pathology; 2000 - Chemistry;
Goals / Objectives
Light and electron microscopes and analytical ancillary equipment like X-ray microanalysis are essential technologies for biological and non-biological materials in agricultural research. For over 30 years the Minnesota Agricultural Experiment Station (MAES), Academic Health Center (AHC) and College of Biological Sciences (CBS) have each supported multiple-user shared imaging core facilities. These efforts benefit basic agricultural research which relies on continued advancement and expansion of imaging infrastructure. Now that the equipment and resources have merged, equipment and staff have expanded to assist users with state-of-the-art techniques in phosphorimaging, chemifluorescence, confocal, live cell and tissue imaging, hyperspectral imaging, light and electron microscopy and expert consulting. These services are made available on a fee-for-use basis to other University of Minnesota laboratories, as well as dozens of industrial research partners. Income from user fees augments the operation. In the past several years these imaging technologies have been expanding research methodologies to better understand and safeguard the food supply. Applying scientific principles to the food system aids in the understanding of food production and safety issues through controls on inputs, changes to production and preparatory processes. The UIC facilitates basic and multi-disciplinary applied research aimed at evaluating options for improving animal health and food safety through microscopic technologies. This project revision continues MAES support funding, and ensures access to state-of-the-art equipment and staff for agricultural research.
Project Methods
The UIC is a collection of shared state-of-the-art core facilities for the imaging of biological specimens utilizing light microscopy; scanning and transmission electron microscopy; digital imaging analysis, visualization and printing; related to research, training and education. Since its inception, the Imaging Center has served as the premier central facility for high-end imaging for the University community. Briefly, the resources of the Imaging Center have been used in the development of new instruments and techniques; visualization of model systems including Arabidopisis, Medicago, Drosophila, yeast, C. elegans and Chlamydononas; discovery of new viruses and other microbes of plants and animals; the examination of cells and organelles in plant and animal tissue culture; biomedical device and reagent integration; experimental composition of food mixtures and dairy projects, elemental composition and morphology of soil particles and their importance in pollution-related research; the elemental composition of plants as related to practical problems in crop plant physiology and health; deterioration of textiles and forest products; various bioprocessing projects designed to lessen the environmental impact of paper and forest product production; renewable energy projects; nitrogen fixation of plants; alternatives to conventional insecticides; the adherence of pathogenic bacteria to food and food -processing surfaces; analysis of food storage materials; the identification and mapping of fungal chromosomes; and for the in situ identification of the activity of various genes of importance during genetic manipulations of vertebrate and plant cell lines. Our target audience includes participation from K-12 students and teachers; academic researchers at the university and college levels; corporate and industrial partners and governmental agencies. There have been over a dozen Masters or PhD degrees that acknowledged the facility in 2010. In the past year, 230 different projects from over a dozen units have done research using the light and electron microscopy instrumentation totaling over 4700 hours. There were over 50 publications that acknowledged the Imaging Center.

Progress 07/01/11 to 06/30/16

Outputs
Target Audience:The University Imaging Centers (UIC) supports multi-disciplinary applied research through micro to macroscopic imaging technologies aimed at evaluating palnt and animal health and food safety. This project revision continues MAES support funding, and enables researchers access to state-of-the-art equipment and staff in their research efforts. Output includes over 10000 research and instructional posters printed. Over 70 past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life- Fungus, NIH, NCI) relied on our facility. There were multi-day workshops that were sponsored by the UIC that trained 500+ users on pioneering technical methodologies in sample preparation, instrument operations and image analysis. Tours to hundreds of K-12 students and teachers were coordinated and well received. Exhibits at regional and national scientific meetings (Microscopy & MicroAnalysis) and public institutions like the Science Museum of Minnesota, Wiesman Art Museum and local theatres are based on efforts and results from UIC staff and collaborations. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The UIC has hosted multiple training opportunities for users, including: Trained over 300 users in the past year on the UIC's instrumentation. Five (5) 1.5 hour ImageJ/FIJI image analysis workshops, 24 students each session. Two (2) 1.5 hour workshop on 3D Image Processing with FIJI, 8/12/15. 20 students registered. • One Nikon Elements image analysis, 1.5 hour workshop,. 20 students • MCCGB graduate training course, Microscopy Fundamentals, Itasca August, 2016. 24 students registered. • Two (2) multi-day day confocal microscopy workshop, Oct. & Dec. • 3 day Microscopy Fundamentals course, Febuary 2016. http://uic.umn.edu/content/uic-workshops-0 How have the results been disseminated to communities of interest?Results of our efforts have been presented at dozens of meetings around the globe. Our workshops, short-courses and symposium were broadly announced and attended by members from over 20 academic departments and a dozen industrial partners. We post infomation about the UIC on a website (www.uic.umn.edu) that had over 20,000 hits last year and contains our reservation system, calendar of events and equipment invormation. We installed a new reservation accounting system, iLab Solutions, traking over 13,000 hours of usage. We are a Nikon Center of Excellence: https://www.nikoninstruments.com/Imaging-Centers/Nikon-Centers-of-Excellence/Nikon-CofE-Locations/Americas/University-of-Minnesota What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? This funding in imaging has contributed toward the expansion of the UIC, permitting the update and disseminate our world-class imaging capabilities at the University of Minnesota and beyond. The University Imaging Centers (UIC), which is charged with meeting the optical imaging needs of the Twin Cities campus, has been able to equip and serve all three separate facilities, the newest of which is located in the Cancer and Cardiovascular Research Building (CCRB) of the Biomedical Discovery District (BDD). This facility is the UIC's third major location and is tailored to the users in the BDD, focusing on fixed and live cell microscopy as well as whole animal imaging. In addition to meeting the needs of existing users, the UIC facilities have proven to be a successful recruiting tool for recent hires in Biomedical Engineering, Chemistry, Integrated Behavioral and Physiology, Neuroscience and the cluster hires in the College of Biological Sciences. Our plans have lead to significant improvements in the intended four main areas: Whole-organism (whole-animal and wholeplant) imaging (100 μm-120 mm), live-cell imaging (200 nm-200 μm), intravital imaging with multiphoton microscopy (200 nm-2 mm) and super-resolution optical imaging (20 nm-20 μm). The UIC was utilized over 12,000 hours by over 350 users in 58 departments in the past fiscal year and operated within budget. This project revision continues MAES support funding, and ensures access to state-of-the-art equipment and staff for agricultural research.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: T. Matsumura, K. Young, Q. Wen, S. Hanany, H. Ishino, Y. Inoue, M. Hazumi, J. Koch, O. Suttman, and V. Sch utz, "Millimeter-Wave Broadband Anti-Reflection Coatings Using Laser Ablation of Sub-Wavelength Structures." Appl. Opt. 55, 3502 (2016).
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2017 Citation: Broadband Millimeter-Wave Anti-Reflection Coatings on Silicon Using Pyramidal Sub-Wavelength Structures Karl Young, Qi Wen, Shaul Hanany, Hiroaki Imada, J�rgen Koch, Tomotake Matsumura, Oliver Suttmann, Viktor Sch�tz
  • Type: Journal Articles Status: Accepted Year Published: 2017 Citation: Bratsch, S., Lockhart B., Mollov D. 2017. Characterization of a Nepovirus causing a leaf mottling disease in Petunia hybrida. Plant Disease.
  • Type: Journal Articles Status: Submitted Year Published: 2017 Citation: Lockhart, B., Mollov D., Olszewski N., Goldsmith N. 2017. Identification, transmission and genomic characterization of a new member of the family Caulimoviridae causing a flower distortion disease of Rudbeckia hirta. Virus Research.
  • Type: Journal Articles Status: Accepted Year Published: 2017 Citation: McLaughlin, M., Lockhart B., Jordan R., Denton G., Mollov D. 2017. Complete nucleotide sequence of Clematis chlorotic mottle virus, a new member of the family Tombusviridae. Archives of Virology.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Wells-Hansen, L. D., Polashock, J. J., Vorsa, N., Lockhart, B. E. L., & McManus, P. S. (2016). Identification of Tobacco streak virus in Cranberry and the Association of TSV with Berry Scarring. Plant Disease, 100(4), 696-703.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Tahir, M. N., Lockhart, B., Grinstead, S., & Mollov, D. (2016). Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing. Archives of Virology, 1-4. DOI: 10.1007/s00705-016-3165-4
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Tahir, M., Lockhart B., Grinstead S., Mollov D. 2016. Nucleotide sequence and genome organization of a novel panicovirus from Bermuda grass. Phytopathology. 106:S4.107.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: K.R. Richert-Poeggeler, B. Lockhart, J.S. Heslop-Harrison and T. Schwarzacher 2016. Viral spread and diversity in Anthropocene. Presented at the International Symposium on Virus Diseases or Ornamental Plants.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Bratsch, S., Lockhart, B., Tisserat, N. Characterization and transmission of a new virus of ash (Fraxinus spp.) in the USA. 3rd International Symposium of Woody Ornamentals of the Temperate Zone. Minneapolis, MN, USA. August 2-6, 2016.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Christenson Wick Z, Leintz CH, Xamonthiene C, Huang BH, Krook-Magnuson E. (2017). Axonal sprouting in commissurally projecting parvalbumin-expressing interneurons. J Neurosci Res. doi:10.1002/jnr.24011
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Verma, M. et al., 2016. Skeletal Muscle Tissue Clearing for LacZ and Fluorescent Reporters, and Immunofluorescence Staining. Methods in molecular biology (Clifton, N.J.), 1460, pp.12940. Available at: http://link.springer.com/10.1007/978-1-4939-3810-0.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Ras oncogene-independent activation of RALB signaling is a targetable mechanism of escape from NRAS(V12) oncogene addiction in acute myeloid leukemia. Pomeroy EJ, Lee LA, Lee RD, Schirm DK, Temiz NA, Ma J, Gruber TA, Diaz-Flores E, Moriarity BS, Downing JR, Shannon KM, Largaespada DA, Eckfeldt CE. Oncogene. 2016 Dec 19. doi: 10.1038/onc.2016.471. [Epub ahead of print]
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Petersen KJ, Goodson HV, Arthur A, Luxton, GWG, Houdusse, A & Titus, MA (2016) MyTH4-FERM myosins have an ancient and conserved role in filopod formation. PNAS, 113:E8059-E8068.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Jaimee Recka,b, Alexandria M. Schauera,c, Kristyn VanderWaal Millsa,d, Raqual Bowera, Douglas Tritschlera, Catherine A. Perronea,e, and Mary E. Portera,* The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Angela K. Spartz, Vai S. Lor, Hong Ren, Neil E. Olszewski, Nathan D. Miller, Guosheng Wu,Edgar P. Spalding, and William M. Gray* Constitutive Expression of Arabidopsis SMALL AUXIN UP RNA19 (SAUR19) in Tomato Confers Auxin-Independent Hypocotyl Elongation1
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Sathoff, A. E., Rajendran, D., Wannemuehler, S. D., Sweeney, K., Manan, F., Kosgey, Z. C., Garber, L. H., and Lockhart, B. E. 2016. First report of a 16SrI (aster yellows) group phytoplasma in phlox in the United States. Plant Health Prog. 17:198-199. doi:10.1094/PHP-BR-16-0021
  • Type: Other Status: Awaiting Publication Year Published: 2016 Citation: Ruth L. Kirschstein National Research Service Award for Predoctoral MD/PhD Fellows, National Institute on Drug Abuse, 2016-2019
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Sommese RF, Ritt M, Swanson CJ, Sivaramakrishnan S. The role of regulatory domains in maintaining auto-inhibition in the multi-domain kinase PKC?. 2017 J Biol Chem (Epub Jan 3) jbc.M116.768457. doi: 10.1074/jbc.M116.768457
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Hove, Mark C., B. E. Sietman, M. Berg, T. Brady, E. Guenther, S. L. Boyer, and D. J. Hornbach. 2016. Early life history of the sheepnose (Plethobasus cyphyus) (Mollusca: Bivalvia). Journal of Natural History 50(9-10): 523-542.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Hove, M., T. Pathoumthong, M. Berg, J. Curtin, C. Chenal, D. Hornbach, B. Jensen, M. Johnson, and E. Livingston. 2016. High school and university researchers study cylindrical papershell (Anodontoides ferussacianus) host fishes. Ellipsaria 18(4): 20-23.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Hove, M., D. Larson, M. Berg, H. Jensen, C. Palmquist, J. Curtin, N. Larsen, N. Klemann, M. Duncan, H. Fiedler, A. Swenson, D. Hornbach, and B. Sietman. 2016. Natural and suitable glochidial hosts for the creek heelsplitter (Lasmigona compressa). Ellipsaria 18(2): 18-22.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Marr, S., B. Sietman, S. Bump, Z. Secrist, M. Hove, E. Haws, M. Berg, A. Ames, L. Drohman, K. Fiedler, J. Knutson, V. Ohnstad, C. Parker, E. Riewestahl, A. Scheunemann, N. Swenson, and A. Taylor. 2016. Determining host fish for the creek heelsplitter (Lasmigona compressa). Ellipsaria 18(1): 19-22.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Theor Appl Genet. 2016 Sep;129(9):1725-38. doi: 10.1007/s00122-016-2735-x. Epub 2016 Jun 9. Fast neutron-induced structural rearrangements at a soybean NAP1 locus result in gnarled trichomes. Campbell BW1, Hofstad AN1, Sreekanta S1, Fu F1, Kono TJ1, O'Rourke JA2, Vance CP1, Muehlbauer GJ1,3, Stupar RM4.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Cancer Res. 2016 Oct 1;76(19):5696-5706. Epub 2016 Aug 8. Adaptive NK Cells with Low TIGIT Expression Are Inherently Resistant to Myeloid-Derived Suppressor Cells. Sarhan D1, Cichocki F1, Zhang B2, Yingst A3, Spellman SR4, Cooley S1, Verneris MR3, Blazar BR3, Miller JS5.


Progress 10/01/14 to 09/30/15

Outputs
Target Audience:The University Imaging Centers (UIC) supports multi-disciplinary applied research through micro to macroscopic imaging technologies aimed at evaluating animal health and food safety. This project revision continues MAES support funding, and enables researchers access to state-of-the-art equipment and staff in their research efforts. Output includes over 3000 research and instructional posters printed. Over 70 past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life- Fungus, NIH, NCI) relied on our facility. There were multi-day workshops that were sponsored by the UIC that trained 100+ users on pioneering technical methodologies in sample preparation, instrument operations and image analysis. Tours to dozens of K-12 students and teachers were coordinated and well received. Exhibits at regional and national scientific meetings (Microscopy & MicroAnalysis) and public institutions like the Science Museum of Minnesota are based on efforts and results from UIC staff and collaborations. Changes/Problems:We have completed the merger by consolidated two accounting units into a single business entity hosted throught the Department of Neuroscience in the Academic Health Centers and Medical School. We will continue to serve the entire research community, especially those familiar with our Snyder Hall, Saint Paul campus facility. The UIC continues to expand University-wide, while the essential funding from this AES program has remained static. The ongoing challenges of funding state-of-the-art facilities such as the UIC continues. What opportunities for training and professional development has the project provided?The UIC has hosted multiple training opportunities for users, including: Three (3) 1.5 hour ImageJ/FIJI image analysis workshops, 6/15/15, 7/7/15 and 8/4/15. 24 students each session. One (1) 1.5 hour workshop on 3D Image Processing with FIJI, 8/12/15. 20 students registered. • One Nikon Elements image analysis, 1.5 hour workshop,. 20 students • MCCGB graduate training course, Microscopy Fundamentals, Itasca August 18- 20, 2015. 24 students registered. • 2.5 day Light sheet microscopy workshop, Sept. 7-9 • Two Confocal Fundamentals short courses date TBD, Oct. and Feb. • 3 day Microscopy Fundamentals course. Date TBD, Febuary 2015. UIC Staff training Dr. Guillermo Marques, Assistant Program Director of the UIC, has attended specialized training as follows:"Optical Microscopy & Imaging in the Biomedical Sciences" course at Marine Biological Laboratory, Woods Hole, MA. September 29-October 9, 2013. "Super-Resolution Localization Workshop", Carl Zeiss Microscopy. Thornwood, NY. June 2-5, 2014."9th LFD Workshop in Advanced Fluorescence Imaging and Dynamics", Laboratory for Fluorescence Dynamics, University of California, Irvine. October 27-31, 2014. National meetings Program Director Sanders was the Co-Chair of the Microscopy and Microanalysis (M&M) meeting in Hartford, CT, Aug. 1-6, 2014 (www.microscopy.org) with short courses and in-week workshops throughout the meeting. (~2,900 attendees of the 11 short courses) as part of the program. Sanders was the Program Chair of the entire M&M meeting in 2015 held Aug. 2-6 in Portland, OR. There were 43 scientific symposia, 11 short courses and about 3,200 attendees (all records). He recruited seven (7) UofM faculty from 5 departments to lead symposia at the meeting. The University of Minnesota was in the top 10 institutions by attendance that participated in the meeting. How have the results been disseminated to communities of interest?Results of our efforts have been presented at dozens of meetings around the globe. Our workshops, short-courses and symposium were broadly announced and attended by members from over 20 academic departments and a dozen industrial partners. We post infomation about the UIC on a website (www.uic.umn.edu) that had over 10,000 hits last year and contains our reservation system, calendar of events and equipment invormation. We installed a new reservation accounting system, iLab Solutions, traking over 13,000 hours of usage. What do you plan to do during the next reporting period to accomplish the goals?The UIC will continue to focus on meeting the imaging needs from across the entire Twin Cities UofMn campus, especially in vitro, in vivo and live cell imaging, and provide related microscopic and computational technologies and expertise. Mark Sanders remains Program Director. Michael O'Connor, Head of the Department of Genetics, Cell Biology andDevelopment, and Timothy Ebner, Head of the Department of Neuroscience, will supervise operations along with a University-wide faculty steering committee to evaluate user needs, prioritize equipment acquisition and upgrades, recommend future staffing, identify funding opportunities, and promote cutting-edge research. Our goals will be to: Maximize the availability of advanced imaging technologies and methodologies to faculty, staff, students and external users. Provide expertise in new imaging technologies to users. Educate the University and broader community about new imaging technologies. Promote interaction between developers of new imaging technology and potential users. We have installed a high-content microscope and a light sheet micorscope for development evaluation of need. The UIC has also been recognized as a Nikon Center of Excellence effective 8/28/15. The web sites have been consolidated and will now reside at http://www.uic.umn.edu

Impacts
What was accomplished under these goals? This funding in imaging has contributed toward the expansion of the UIC, permitting the update and disseminate our world-class imaging capabilities at the University of Minnesota and beyond. The University Imaging Centers (UIC), which is charged with meeting the optical imaging needs of the Twin Cities campus, has been able to equip and serve all three separate facilities, the newest of which is located in the Cancer and Cardiovascular Research Building (CCRB) of the Biomedical Discovery District (BDD). This facility is the UIC's third major location and is tailored to the users in the BDD, focusing on fixed and live cell microscopy as well as whole animal imaging. In addition to meeting the needs of existing users, the UIC facilities have proven to be a successful recruiting tool for recent hires in Biomedical Engineering, Chemistry, Integrated Behavioral and Physiology, Neuroscience and the cluster hires in the College of Biological Sciences. Our plans have lead to significant improvements in the intended four main areas: Whole-organism (whole-animal and whole-plant) imaging (100 μm-120 mm), live-cell imaging (200 nm-200 μm), intravital imaging with multiphoton microscopy (200 nm-2 mm) and super-resolution optical imaging (20 nm-20 μm). The UIC was utilized over 10,000 hours in the past fiscal year and operated within budget. This project revision continues MAES support funding, and ensures access to state-of-the-art equipment and staff for agricultural research.

Publications

  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Schroeder, L., A. Edgcumbe, K. Murphy, S. Bump, A. Londo, B. Sietman, M. Hove, and G. Van Susteren. 2014. Host fishes and glochidia release behavior of Elliptio dilatata. Ellipsaria 16(4): 32-34.
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Sokol, K.A., and Olszewski, N.E. (2015). The Putative Eukaryote-Like O-GlcNAc Transferase of the Cyanobacterium Synechococcus elongatus PCC 7942 Hydrolyzes UDP-GlcNAc and Is Involved in Multiple Cellular Processes. Journal of bacteriology 197, 354-361.
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis Hong Xia ; Vidya Bodempudi ; Alexey Benyumov ; Polla Hergert ; Damien Tank ; Jeremy Herrera ; Jeff Braziunas ; Ola Larsson ; Matthew Parker ; Daniel Rossi ; Karen Smith ; Mark Peterson ; Andrew Limper ; Jose Jessurun ; John Connett ; David Ingbar ; Sem Phan ; Peter B. Bitterman ; Craig A. Henke The American journal of pathology. 2014; 184(5): 1369-1383.
  • Type: Journal Articles Status: Submitted Year Published: 2015 Citation: Viktor Sch�tz, J�rgen Koch, Oliver Suttmann, Ludger Overmeyer, Tomotake Matsumura, Shaul Hanany, Qi Wen and Karl Young. "Laser processing of sub-wavelength structures on sapphire and alumina for millimeter wavelength broadband anti-reflection coatings". Journal of Laser Micro/Nanoengineering.
  • Type: Journal Articles Status: Submitted Year Published: 2015 Citation: Karl Young, Shaul Hanany, Qi Wen, Tomotake Matsumura, Viktor Sch�tz. "Millimeter wavelength broadband anti-reflection coatings using laser machined sub-wavelength structures". Applied Optics.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Orland I. J., Edwards R. L., Cheng H., Kozdon R., Cross M., and Valley J. W. (2015) Direct measurements of deglacial monsoon strength in a Chinese stalagmite. Geology 43, 555-558
  • Type: Journal Articles Status: Submitted Year Published: 2015 Citation: Anirudh Sharma, Mike D. DiVito, Daniel E. Shore, Andrew D. Block, Katie M. Pollock, Bethanie J.H. Stadler, Cornelius Lam, and Allison Hubel, Influence of nanowire-oriented matrices on behavior of arachnoid cells, Submitted to BioMaterials (2015).
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: V Thayanithy, V Babatunde, EL Dickson, P Wong, S Oh, X Ke, A Barlas, S Fujisawa, Y Romin, AL Moreira, RJ Downey, CJ Steer, S Subramanian, K Manova-Todorova, MA Moore, E Lou. Tumor exosomes induce tunneling nanotubes in lipid raft-enriched regions of human mesothelioma cells. Experimental Cell Research, Epub ahead of print January 24 2014; pii: S0014-4827(14)00023-8. doi: 10.1016/j.yexcr.2014.01.014
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Jesus Cantu-Valle, Israel Betancourt, John E. Sanchez, Francisco Ruiz-Zepeda, Mazin M. Maqableh, Fernando, Mendoza-Santoyo, Bethanie J. H. Stadler, and Arturo Ponce, Mapping the magnetic and crystal structure in cobalt nanowires Journal of Applied Physics 118 024302 (2015). doi:10.1063/1.4923745
  • Type: Journal Articles Status: Submitted Year Published: 2015 Citation: Wu, X.; Newbold, M. A.; Gao, Z.; Haynes, C. L. A Versatile Microfluidic Platform for the Study of Angiogenesis and Inflammation, submitted to Analyst.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: V Thayanithy, EL Dickson, C Steer, S Subramanian, E Lou. Tumor-stromal cross talk: direct cell-to-cell transfer of oncogenic microRNAs via tunneling nanotubes. Translational Research, Epub ahead of print May 24, 2014.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Narayanan, P., Chatterton, P., Ikeda A., Ikeda S., Corey, D.P., Ervasti, J.M. and Perrin B.J. (2015) Length regulation of mechanosensitive stereocilia depends on very slow actin dynamics and filament severing proteins. Nat. Commun. 6:6855.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Kim, S., Spike, C., & Greenstein D. (2013). Control of oocyte growth and meiotic maturation in C. elegans. C. elegans germline development (T. Schedl, ed.), in: Advances in experimental medicine and biology (Springer, New York) 757: 277-320.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Oldenbroek, M., Robertson, S. M. Guven-Ozkan, T., Spike, C., Greenstein, D., & Lin, R. (2013). Regulation of maternal Wnt mRNA translation in C. elegans embryos. Development 140: 4614-4623.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Starich, T. A., Hall, D. H., & Greenstein, D. (2014). Two classes of gap junction channels mediate soma-germline interactions essential for germline proliferation and gametogenesis in Caenorhabditis elegans. Genetics 198: 1127-1153.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Spike, C. A., Coetzee, D., Nishi, Y., Guven-Ozkan, T., Oldenbroek, M., Yamamoto, I., Lin, R., & Greenstein, D. (2014). Translational control of the oogenic program by components of OMA ribonucleoprotein particles in Caenorhabditis elegans. Genetics 198: 1513-1533.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Spike, C. A., Coetzee, D., Eichten, C., Wang, X., Hansen, D., & Greenstein, D. (2014). The TRIM-NHL protein LIN-41 and the OMA RNA-binding proteins antagonistically control the prophase-to-metaphase transition and growth of C. elegans oocytes. Genetics 198: 1535-1558.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Yamanaka, N., Marques, G., and O'Connor, M.B. Vesicle mediated steroid hormone release in Drosophila Cell (Nov 5 issue) 2015
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Raybuck JD and Thayer SA (2015) GluN2B-containing NMDA receptor antagonist ifenprodil produces long-lasting increase in dendritic spine density: Within-subject multiphoton imaging. Society for Neuroscience Abstract 204.15
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Godinho, S.A., Picone, R., Burute, M., Dagher, R., Su, Y., Leung, C.T., Polyak, K., Brugge, J.S., Th�ry, M., Pellman D. Oncogene-like induction of cellular invasion from centrosome amplification. Nature. 510, 167-71. (2014). PMCID: PMC4061398.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Klos, K.S., Warmka, J.K., Drachenberg, D.M., Chang, L., Luxton, G.W., Leung, C.T., Schwertfeger, K.L., Wattenberg, E.V. Building bridges toward invasion: tumor promoter treatment induces a novel protein kinase C-dependent phenotype in MCF10A mammary cell acini. PLoS One. 9(3), (2014). PMCID: PMC3944432.
  • Type: Journal Articles Status: Accepted Year Published: 2013 Citation: Leung. C.T. Epithelial cell translocation: New insights into mechanisms of tumor initiation. Bioessays. 35(2), 80-3 (2013). PMCID: PMC3732789
  • Type: Journal Articles Status: Accepted Year Published: 2012 Citation: Leung, C.T., and Brugge, J.S. Outgrowth of single oncogene-expressing cells from suppressive epithelial environments. Nature. 482, 410-413 (2012). PMCID: PMC3297696.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Kistler, H.C. and Broz, K. 2015. Cellular compartmentalization of secondary metabolism. Frontiers in Microbiology 6:68 doi:10.3389/fmicb.2015.00068.
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Armbrust KR, Wang X, Hathorn TJ, Cramer SW, Chen G, Zu T, Kangas T, Zink AN, �z G, Ebner TJ, Ranum LP. Mutant ?-III spectrin causes mGluR1? mislocalization and functional deficits in a mouse model of spinocerebellar ataxia type 5. J Neurosci 34(30):9891-904, 2014. PMCID:PMC4107406
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Kim JH, Frantz AM, Anderson KL, Graef AJ, Scott MC, Robinson SR, Sharkey LC, OBrien TD, Dickerson EB, Modiano JF. (2014). Interleukin-8 promotes canine hemangiosarcoma growth by regulating the tumor microenvironment. Exp Cell Res, 323(1), 155-164. PMID: 24582862.
  • Type: Journal Articles Status: Accepted Year Published: 2013 Citation: Lane AB, Gim�nez-Abi�n JF and Clarke DJ (2013). A novel Chromatin Tether domain controls topoisomerase II? dynamics and mitotic chromosome formation. J. Cell Biology 203:471-486. doi:10.1083/jcb.201303045.
  • Type: Journal Articles Status: Accepted Year Published: 2013 Citation: Menke, J., Weber, J., Broz, K. and Kistler, H.C. 2013. Cellular development associated with induced mycotoxin synthesis in the filamentous fungus Fusarium graminearum. PLoS ONE 8(5): e63077.
  • Type: Journal Articles Status: Accepted Year Published: 2012 Citation: Menke, J., Dong, Y., and Kistler, H.C. 2012. Fusarium graminearum Tri12p influences virulence to wheat and trichothecene accumulation. Molecular Plant-Microbe Interactions 25:14081418.
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Hammond, J., Mollov, D., Reinsel, M., and Lockhart, B. 2014. Detection of Helenium virus S and two distinct isolates of Butterbur mosaic virus in a single plant of Veronica.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Sharma, Anirudh, Gregory M. Orlowski, Yuechen Zhu, Daniel Shore, Seung Yeon Kim, Michael D. DiVito, Allison Hubel, and Bethanie JH Stadler. "Inducing cells to disperse nickel nanowires via integrin-mediated responses." Nanotechnology 26, no. 13 (2015): 135102.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Floudas, D. B. W. Held, R. Riley, L. G. Nagy, G. Koehler, A. S. Ransdell, H. Younus, J. Chow, J. Chiniquy, A. Lipzen, A. Tritt, H. Sun, S. Haridas, K. LaButti, R. A. Ohm, U. Ku?es, R. A. Blanchette, I. V. Grigoriev, R. E. Minto, D. S. Hibbett. 2015. Evolution of novel wood decay mechanisms in Agaricales revealed by the genome sequences of Fistulina hepatica and Cylindrobasidium torrendii. Fungal Genetics and Biology 76:78- 92.
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Hori, C, T. Ishida, K. Igarashi, M. Samejima, H. Suzuki, E. Master, P. Ferreira, F. J. Ruiz-Duenas, B. Held, P. Canessa, L. F. Larrondo, M. Schmoll, I. S. Druzhinina, C. P. Kubicek, J. A. Gaskell, P. Kersten, F. St. John, J. Glasner, G. Sabat, S. Splinter BonDurant, K. Syed, J. Yadav, A. C. Mgbeahuruike, A. Kovalchuk, F. O. Asiegbu, G. Lackner, D. Hoffmeister, J. Rencoret, A. Gutie rrez, H. Sun, E. Lindquist, K. Barry, R. Riley, I. V. Grigoriev, B. Henrissat, U. Kues, R. M. Berka, A. T. Mart?nez, S. F. Covert, R. A. Blanchette and D. Cullen. 2014. Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood. PLoS Genetics 10(12): e1004759. doi:10.1371/journal.pgen.1004759.
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Oritz, R., M. Parraga, J. Navarrete, I. Carrasco, E. de la Vega, M. Ortiz, P. Herrera, J. A. Jurgens, B. W. Held and R. A. Blanchette. 2014. Investigations of Biodeterioration by Fungi in Historic Wooden Churches of Chiloe, Chile. Microbial Ecology 67:568-575
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Ortiz, R. and Robert A. Blanchette. 2014. Desfibrado de Maderas obtenidas desde las oficinas salitreras de Humberstone y Santa Laura en Chile. Un sitio del patrimoniomundial (Defibration wood obtained from the Saltpeter Works Humberstone and Santa Laura in Chile. World Heritage Sites). Madera y Bosques 20(3):59-58.
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Gaskell, J., A. Marty, M. Mozuch, P. J. Kersten, S. Splinter BonDurant, G. Sabat, A. Azarpira, J. Ralph, O. Skyba, S. D. Mansfield, R. A. Blanchette, and D. Cullen. 2014. Influence of Populus genotype on gene expression by the wood decay fungus Phanerochaete chrysosporium. Applied and Environmental Microbiology 80:5828-5835
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Riley, R., A. A. Salamov, D. W. Brown, L. G. Nagy, D. Floudas, B. W. Held, A. Levasseur, V. Lombard, E. Morin, R. Otillar, E. A. Lindquist, H. Sun, K. M. LaButti, J. Schmutz, D. Jabbour, H. Luo, S. E. Baker, A. G. Pisabarro, J. D. Walton, R. A. Blanchette, B. Henrissat, F. Martin, D. Cullen, D. S. Hibbett, and I. V. Grigoriev. Extensive sampling of basidiomycete genomes demonstrates inadequacy of the whiterot/ brown-rot paradigm for wood decay fungi. 2014. Proceedings of the National Academy of Sciences 111:9923-9928
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Ortiz, R., H. Navarretea, J. Navarrete, M. P�rragac, I. Carrasco,E. de la Vegac, M. Ortiz, P. Herrera, and R. A. Blanchette. 2014 Deterioration, decay and identification of fungi isolated from wooden structures at the Humberstone and Santa Laura saltpeter works: A world heritage site in Chile. International Biodeterioration and Biodegradation 86:309- 316
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Scott MC, Sarver AL, Tomiyasu H, Van Etten J, Varshney J, Subramanian S, Modiano JF. (2015). Aberrant RB-E2F transcriptional regulation defines molecular phenotypes of osteosarcoma. J Biol Chem, in revision.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Modiano JF, Lindborg BA, McElmurry RT, Lewellen M, Forster CL, Zamora EA, Schaack J, Bellgrau D, OBrien TD, Tolar J. (2015). Mesenchymal stromal cells inhibit anti-tumor immune responses by attenuating inflammation and reorganizing the tumor microenvironment. Cancer Immunol Immunother, 2015 Aug 7. [Epub ahead of print]. PMID: 26250807.
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Mollov, D.S., Guaragna, M., Lockhart, B., Rezende, J., Jordan, R. 2014. First report of Catharanthus mosaic virus in Mandevilla in the United States. Plant Disease. 99:165.
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Bratsch, S., Flynn, J., Lockhart, B. (2014) Detection of Tobacco Rattle Virus (TRV) in Phryma leptostachya L.: A new native plant host in Minnesota. Plant Health Progress, 15:4, 184-185. Online at: https://www.plantmanagementnetwork.org/sub/php/volume15/number4/PHP-BR-14-0021.pdf
  • Type: Journal Articles Status: Accepted Year Published: 2014 Citation: Lockhart, B., Mollov, D.S., Mason, S., Bratsch, S. 2014. First report of natural occurrence of Turnip vein-clearing virus in garlic mustard (Alliaria petiolata) in the United States. Plant Health Progress. doi:10.1094/PHP-BR-14-0029.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Agneroh, T., Bratsch, S., Lockhart, B. (2015) First report of Canna yellow mottle virus in Kenya. Plant Health Progress 16:1, 34-35. Online at: https://www.plantmanagementnetwork.org/sub/php/volume16/number1/PHP-BR-14-0037.pdf
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Bratsch, S., Mollov, D., Lockhart, B., Johnson, D., Ehlenbeck, S. (2015). First report of Cucumber Mosaic Virus Infection in Pachysandra in the U.S.A.. Plant Disease 99:3, 422.1. Online at: http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-09-14-0974-PDN
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: Bratsch, S., Lockhart, B., Johnson, D. (In preparation) First report of Alternanthera Mosaic Virus in Penstemon in the U.S.A. Plant Health Progress.
  • Type: Journal Articles Status: Accepted Year Published: 2105 Citation: Wells-Hansen, L.D., Polashock, J.J., Vorsa, N., Lockhart, B., and McManus, P. 2015. Identification of Tobacco streak virus in cranberry and the association of TSV with berry scarring. Plant Disease First Look. Online.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Mahuku, G., Lockhart, B.E., Wanjala, B., Jones, M.W., Kimunye, J.N., Stewart, L.R., Cassone, B.J., Sevgan, S., Nyasani, J.O., Kusia, E., Kumar, P.L., Niblett, C.L., Kiggundu, A., Asea, G., Pappu, H.R., Wangai, A., Prasanna, B.M., and Redingbaugh, M.G. 2015.Maize Lethal Necrosis (MLN), an emerging threat to Maize-Based Food Security in Sub-Saharan Africa. Phytopathology: 105:956-965.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Bratsch, S., Mollov, D., Lockhart, B., Johnson, D., and Ehlenbeck, S. 2015. First report of Cucumber mosaic virus infection in Pachysandra in the United States. Plant Disease: 99(1).
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Vo, J.N., Mahfuz, N.N., Lockhart, B.E., Geering, A.D.W. 2015. Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection. European Journal of Plant Pathology: 143(3): 619-626.
  • Type: Theses/Dissertations Status: Accepted Year Published: 2015 Citation: Physical Determinants of Glioma Cell Migration and Disease Progression Rebecca Klank
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: The predicted role of steric specificity in crowding-mediated effects on reversible biomolecular association. Powers JD, Castle BT, Odde DJ. Phys Biol. 2015 Nov 23;12(6):066004. doi: 10.1088/1478-3975/12/6/066004. PMID: 26595211
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles. McCoy KM, Tubman ES, Claas A, Tank D, Clancy SA, O'Toole ET, Berman J, Odde DJ. Mol Biol Cell. 2015 Nov 5;26(22):3999-4014. doi: 10.1091/mbc.E14-10-1454. Epub 2015 Sep 9.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2015 Citation: Sanders, M.A., Microscopy and Microanlysis 2015. Microscopy Today, vol 24, No.1.
  • Type: Journal Articles Status: Accepted Year Published: 2015 Citation: Plasmon resonance and the imaging of metal-impregnated neurons with the laser scanning confocal microscope. Thompson KJ, Harley CM, Barthel GM, Sanders MA, Mesce KA. eLife. 2015; 4. PubMed [journal]PMID: 26670545 PMCID: PMC4718721


Progress 10/01/13 to 09/30/14

Outputs
Target Audience: The University Imaging Centers (UIC) supports multi-disciplinary applied research through micro to macroscopic imaging technologies aimed at evaluating animal health and food safety. This project revision continues MAES support funding, and ensures researchers access to state-of-the-art equipment and staff in their research efforts. There have been over 3000 research and instructional posters printed. Past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life- Fungus, NIH, NCI) relied on our facility. There were multi-day workshops that were sponsored by the UIC that trained 100+ users on pioneering technical methodologies in sample preparation and image analysis. Tours to dozens of K-12 students and teachers were well received. Exhibits at regional and national scientific meetings and public institutions like the Science Museum of Minnesota are based on efforts and results from UIC staff and collaborations. Changes/Problems: The UIC continues to expand University-wide, while the essential funding from this AES program have remained static or decreased. The ongoing challenges of funding state-of-the-art facilities such as the UIC continues. What opportunities for training and professional development has the project provided? We hosted multiple workshops on advanced image processing with 82 participants. Staff attended local and natioinal microscopy MMS and M&M meetings. Staff attended the Advance Fluorescence Lifetime Imaging Workshop at UC Davis. The UIC co-hosted two iPrime events; "3D Correlative Micoscopy" and Futures in "Imaging", each with over 30 attendess from both academia and industry. How have the results been disseminated to communities of interest? Results of our efforts have been presented at dozens of meetings around the globe. Our workshops and short-courses were broadly announced and attended by members from over 10 academic and industrial partners. We post infomation about the UIC on a website (www.uic.umn.edu) that had over 10,000 hits last year and contains our reservation system, calendar of events and equipment invormation. We installed a new reservation accounting system, iLab Solution, this past year. What do you plan to do during the next reporting period to accomplish the goals? The UIC will focus on meeting the imaging needs from across the entire Twin Cities UofMn campus, especially in vitro, in vivo and live cell imaging, and provide related microscopic and computational technologies and expertise. Mark Sanders remains Program Director. Michael O'Connor, Head of the Department of Genetics, Cell Biology andDevelopment, and Timothy Ebner, Head of the Department of Neuroscience, will supervise operations along with a University-wide faculty steering committee to evaluate user needs, prioritize equipment acquisition and upgrades, recommend future staffing, identify funding opportunities, and promote cutting-edge research. Our goals will be to: Maximize the availability of advanced imaging technologies and methodologies to faculty, staff, students and external users. Provide expertise in new imaging technologies to users. Educate the University and broader community about new imaging technologies. Promote interaction between developers of new imaging technology and potential users. We have installed a multi-photon confocal microscope and a super-resolution micorscope at their respective facilities. The UIC has also been recognized as a Nikon Center of Excellence effective 12/17/13. The web sites have been consolidated and will now reside at http://www.uic.umn.edu.

Impacts
What was accomplished under these goals? The University Imaging Centers (UIC) supports basic and multi-disciplinary applied research through microscopic imaging technologies aimed at evaluating options for improving plant and animal health and food safety. This project revision continues MAES support funding, and helps to ensure researchers access to state-of-the-art equipment and staff in these research efforts. There have been over 1200 research and instructional posters printed. There have been six Graduate degrees that acknowledged the facility in 2014. Past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life-Fungus, UofMn I3 and NIH G20) relied on our facility. There was a multi-day workshops hosted by the UIC that trained participants on pioneering technical methodologies in sample preparation and fundimentals of imaging. Tours over one hundred K thru 12 students and teachers were hosted. New exhibits at the Science Museum of Minnesota are based on results from staff and collaborations. In the past year, 420+ different projects from seventy-two units (21 in St. Paul) have done research using the staff, light and electron microscopy instrumentation totaling over 6000 hours at our 3 locations. There were over 20 publications that acknowledged the Imaging Center. Additional activities include: 10+ invited presentations by UIC staff.

Publications

  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Leonard, P., M. Hove, B. Davis, E. Wanner, G. Van Susteren, B. Sietman, M. Berg, A. Handy, A. Thoreen, K. Rod, M. Hanson, R. Pochman, S. Nelson, W. LaMere, and D. Hornbach. 2014. Flutedshell (Lasmigona costata) natural hosts and glochidia release behavior. Ellipsaria 16(4): 29-32.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Schroeder, L., A. Edgcumbe, K. Murphy, S. Bump, A. Londo, B. Sietman, M. Hove, and G. Van Susteren. 2014. Host fishes and glochidia release behavior of Elliptio dilatata. Ellipsaria 16(4): 32-34.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Hove, M., B. Davis, E. Wanner, P. Leonard, G. Van Susteren, B. Sietman, S. Bump, S. Marr, K. Murphy, M. Berg, A. Handy, A. Thoreen, K. Rod, M. Hanson, R. Pochman, S. Nelson, and W. LaMere. 2014. Laboratories show Lasmigona costata metamorphose on several fish species. Ellipsaria 16(2): 21-23.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Sokol, K.A., and Olszewski, N.E. (2015). The Putative Eukaryote-Like O-GlcNAc Transferase of the Cyanobacterium Synechococcus elongatus PCC 7942 Hydrolyzes UDP-GlcNAc and Is Involved in Multiple Cellular Processes. Journal of bacteriology 197, 354-361.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2014 Citation: Mishler E, Reicks M. Dietary intake, attitudinal, and contextual differences in snacking occasions characterized as indulgent by midlife women. 11th Annual Women's Health Research Conference, University of Minnesota, September 29, 2014
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Reicks M, Leak TM, Swenson A, Vickers Z. Using in-home behavioral economic strategies and enhanced food preparation skills to Increase vegetable intake and variety among children. J Nutr Educ Behav. 2014;46(4): S197.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Lakanen A, Reicks M. Involving children in meal preparation: Implications for increasing vegetable intake at home and school. FASEB J. 2014;28:808.9. Experimental Biology Annual Meeting. San Diego, CA, April 2014.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Berkowitz S, Mykerezi E, Reicks M, Degeneffe D, Marquart L. Providing flexible food portions in a restaurant setting: Impact on business operations, food consumption and food waste. FASEB J 2014;28:390.3. Experimental Biology Annual Meeting. San Diego, CA, April 2014.
  • Type: Book Chapters Status: Published Year Published: 2014 Citation: M.C. Aime, M. Toome and D.J. McLaughlin. 2014. Pucciniomycotina. In: D.J. McLaughlin and J.S. Spatafora (eds) The Mycota, Vol. VII, A, Systematics and evolution. Springer, Berlin, pp. 271-294.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Leak TM, Swenson A, Vickers Z, Reicks M. Feasibility and effectiveness of in-home behavioral economic strategies to increase vegetable intake among low-income children. FASEB J. 2014;28:808.18. Experimental Biology Annual Meeting. San Diego, CA, April 2014.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Mollov, D., M.A. Guaragna, B. Lockhart, J.A.M. Rezende, and R. Jordan. 2014. First report of Catharanthus mosaic virus in Mandevilla in the United States. Plant Disease. Online First: October 1.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Lockhart, B., D. Mollov, S. Mason and S. Bratsch. 2014. First report of natural occurrence of Turnip vein-clearing virus in garlic mustard (Alliaria petiolata) in the United States. Plant Health Progress. doi:10.1094/PHP-BR-14-0029
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Mollov, D., B. Lockhart, D. C. Zlesak. 2014. Complete nucleotide sequence of Rose yellow leaf virus, a new member of the family Tombusviridae. 159:2795-2798.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Mollov, D., B. Lockhart, E. Saalau-Rojas, C. Rosen. 2014. First report of a 16SrI (Aster Yellows) Group Phytoplasma on garlic (Allium sativum) in the USA. Plant Disease 98:419.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Bratsch, S.A., Flynn, J.S., Lockhart, B.E. 2014. Detection of Tobacco rattle virus (TRV) in Phryma leptostachya L.: A new native plant host in Minnesota. Plant Health Progress doi:10.1094/PHP-BR-14-0021.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Colson BA, Petersen KJ, Collins BC, Lowe DA, Thomas DDThe myosin super-relaxed state is disrupted by estradiol deficiency. Biochem Biophys Res Commun. 2015 Jan 2;456(1):151-5. doi: 10.1016/j.bbrc.2014.11.050. Epub 2014 Nov 21.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Angela K. Spartz, Hong Ren, Mee Yeon Park,Kristin N. Grandt, Sang Ho Lee, Angus S. Murphy, Michael R. Sussman, Paul J. Overvoord and William M. Gray. SAUR Inhibition of PP2C-D Phosphatases Activates Plasma Membrane H+-ATPases to Promote Cell Expansion in Arabidopsis. The Plant Cell, Vol. 26: 21292142, May 2014.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: A.T. Vogel, G. Marqu�s and M.A. Sanders (2013). Microwave-assisted Fixation, Labeling and Clearing for Optical Microscopy of Thick Specimens. Microscopy and Microanalysis, 19 (Suppl. 2) , pp 16-17. doi:10.1017/S1431927613002079
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: David J. McLaughlin, Rosanne A. Healy, Robert W. Roberson, T. K. Arun Kumar and Gail J. Celio. 2014. Does mitosis in the zygomycetous fungus Coemansia reversa provide evidence for fungal spindle pole body evolution? 10th International Mycological Congress, Bangkok, Thailand.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Mollov, D. B. Lockhart, A. Phibbs, T. Creswell, G. Ruhl, E. Dorman, G. Kinard, and R. Jordan. 2014. Clematis chlorotic mottle virus, a novel virus occurring in clematis in the USA. Phytopathology 104:S3.81.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Craig E Eckfeldt, MD, PhD1,2, Emily J Pomeroy, MS1,2*, Robin DW Lee1,2*, Katherine S Hazen1,2*, Branden S Moriarity, PhD2,3* and David A Largaespada, PhD2,3. Ras Effector Pathways Differentially Support Proliferation and Survival of Acute Myeloid Leukemia. Session: 616. Acute Myeloid Leukemia: Novel Therapy, excluding Transplantation: Poster III


Progress 01/01/13 to 09/30/13

Outputs
Target Audience: Our target audience includes 24/7/365 availability to researchers at the undergraduate, graduate, post-doctoral and faculty levels at the University of Minnesota. We also have significant participation from regional K-12 students and teachers; academic researchers from other universities and colleges; corporate and industrial partners and governmental agencies. We hosted 3 multi-day workshops on sample preparation, advanced microscopy and image formation. Staff presented in 2 national meetings. We were part of Microscope Day at the Science Museum of Minnesota in Nov. 2013 where we had over 300 visitors from the general public. Mark Sanders continues on the council of the Histochemical Society on the awards committee and Director of Biology for the Microscopy Society of America's Council. Changes/Problems: The UIC continues to expand, while the essential funding from this AES program have remained static or decreased. The ongoing challenges of funding state-of-the-art facilities such as this continue. What opportunities for training and professional development has the project provided? We hosted a multiday workshop on advanced light microscopy with 32 participants. We co-hosted two short courses with the Mayo Clinic Foundation, one on multi-photon microcsopy and one on super-resolution microscopy that attracted over 100 registrants from both locations. Staff attended local and natioinal microscopy MMS and M&M meetings. Staff attended the Advance Qunatitative Light Microscopy Course at Marine Biological Laboratory at Woods Hole, MA. The UIC co-hosted two iPrime events; "3D Correlative Micoscopy" and Futures in "Imaging", each with over 30 attendess from both academia and industry. How have the results been disseminated to communities of interest? Results of our efforts have been presented at dozens of meetings around the globe. Our workshops and short-courses were broadly announced and attended by members from over 10 academic and industrial partners. At least one high school teacher participated as well. We post infomation about the UIC on a website (www.uic.umn.edu) that had over 10,000 hits last year and contains our reservation system, calendar of events and equipment invormation. What do you plan to do during the next reporting period to accomplish the goals? The UIC will focus on meeting the imaging needsfrom across the entire Twin Cities UofMn campus, especially in vitro, in vivo and live cell imaging, and provide related microscopic and computational technologies and expertise. Mark Sanders has been appointed Program Director. Michael O'Connor, Head of the Department of Genetics, Cell Biology andDevelopment, and Timothy Ebner, Head of the Department of Neuroscience, will supervise operations along with a University-wide faculty steering committee to evaluate user needs, prioritize equipment acquisition and upgrades, recommend future staffing, identify funding opportunities, and promote cutting-edge research. Our goals will be to: Maximize the availability of advanced imaging technologies and methodologies to faculty, staff, students and external users. Provide expertise in new imaging technologies to users. Educate the University and broader community about new imaging technologies. Promote interaction between developers of new imaging technology and potential users. The first floor of Jackson Hall was recently remodeled to house the Minneapolis facilities thanks to a $6.2 million NIH G20 grant secured by several departments and programs. The facility was also awarded a MN Parnership Grant with Drs. Ebner and Salisbury and has installed a multi-photon confocal microscope and a super-resolution micorscope at their respective facilities. The UIC has also been recognized as a Nikon Center of Excellence effective 12/17/13. The web sites have been consolidated and will now reside at http://www.uic.umn.edu.

Impacts
What was accomplished under these goals? The UIC supports basic and multi-disciplinary applied research through microscopic imaging technologies aimed at evaluating options for improving plant and animal health and food safety. This project revision continues MAES support funding, and helps to ensure researchers access to state-of-the-art equipment and staff in these research efforts. There have been over 800 research and instructional posters printed. There have been eight Graduate degrees that acknowledged the facility in 2013. Past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life-Fungus, UofMn I3 and NIH G20) relied on our facility. There were 3 multi-day workshops hosted by the UIC that trained participants on pioneering technical methodologies in sample preparation and fundimentals of imaging. Tours over one hundred K thru 12 students and teachers were hosted. New exhibits at the Science Museum of Minnesota are based on results from staff and collaborations. In the past year, 400+ different projects from seventy-nine units (23 in St. Paul) have done research using the staff, light and electron microscopy instrumentation totaling over 5000 hours at our 3 locations. There were over 20 publications that acknowledged the Imaging Center. Additional activities include: 10+ invited presentations by UIC staff.

Publications


    Progress 01/01/12 to 12/31/12

    Outputs
    OUTPUTS: The two main objectives for this project are: 1) to support and maintain the core microscopy facilities known as the University Imaging Centers (UIC) for the benefit and enhancement of MAES sponsored agricultural research, 2) to provide scientific and technical guidance, assistance, and consultation on the application and use of light and electron optics and related technologies for the solution of both applied and fundamental research problems. The UIC has developed new instruments and techniques and aided in the visualization of valuable genetic and agricultural model systems including Arabidopisis, Medicago, Drosophila, yeast, Maize, Oryza, C. elegans and Chlamydononas which has lead to a host of research discoveries, including: new viruses and diseases; examination of cells and organelles in plant and animal tissues; biomedical device and reagent integration; experimental composition of food mixtures and dairy projects; elemental composition and morphology of soil particles and their importance in pollution-related research; elemental composition of plants as related to practical problems in crop plant physiology and health; deterioration of textiles and forest products; various bioprocessing projects designed to lessen the environmental impact of paper and forest product production; renewable energy projects; nitrogen fixation of plants; alternatives to conventional insecticides; adherence of pathogenic bacteria to food and food -processing surfaces; analysis of food storage materials; identification and mapping of fungal chromosomes; and the in situ identification of the activity of various genes of importance during genetic manipulations of vertebrate and plants. Since its inception, the UIC has served as the premier central facility for high-end imaging for the entire University community, serving over 450 users in the past fiscal year. Our target audience includes participation from K to 12 students and teachers; academic researchers at the university and college levels; corporate and industrial partners and governmental agencies. To make this multiple-user facility productive requires an environment of user cooperation, and guidelines for usage. PARTICIPANTS: General policy is provided by, Mark Sanders, Program Director, through a 13 member Advisory Committee and is reinforced through constant contact with project leaders and users. The St. Paul facility of the UIC is staffed by Dr. Gail Ceilo, Mr. Adam Vogel, Mr. Alex Cramer, Ms. Chemi Banari, and Ms. Ashleigh Skaalen. In addition to the AES funding, significant subsidy (~30%) toward the cost of operating is provided by the College of Biological Sciences, Academic Health Center and OVPR. User fees (reviewed annually) are posted on our website (www.uic.umn.edu) subsidize operational costs. The facility is planned to operate under a cost neutral budget. PARTICIPANTS: Mark Sanders, UIC Program Director. The facility is staffed by Dr. Gail Ceilo, Mr. Adam Vogel and Mr. Alex Cramer along with student workers, Ms. Chemi Banari, and Ms. Ashleigh Skaalen. Collaboration MN Partnership: Dr. Jeffery Salisbury, Mayo Clinic Foundation. We have hosted short courses in Advanced Sample Preparation for Imaging and Fundimentals of Microscopy. Director Sanders has a faculty of Histochemical Society short course at Marine Biology Laboratory at Woods Hole, MA. Celio participated in EDS/EDXS short course associated with the Microscopy Society of America Annual Meeting. Sanders was elected Director of Biology for Microscopy Society of America Council. TARGET AUDIENCES: Our target audience includes active usage by University researchers at the undergraduate, graduate, post-doctoral and faculty levels. We also have significant participation from regional K-12 students and teachers; academic researchers from other universities and colleges; corporate and industrial partners and governmental agencies. We hosted 2 workshops on sample preparation and image formation. Staff presented in 2 national meetings. Mark Sanders is on the council of the Histochemical Society on the awards committee and Director of Biology for the Microscopy Society of America's Council. PROJECT MODIFICATIONS: The College of Biological Sciences (CBS) and Academic Health Center/Medical School have merged two facilities to form the University Imaging Centers (UIC). The UIC will focus on meeting the imaging needsfrom across the entire Twin Cities campus, especially in vitro, in vivo and live cell imaging, and provide related microscopic and computational technologies and expertise. Mark Sanders has been appointed Program Director. Michael O'Connor, Head of the Department of Genetics, Cell Biology andDevelopment, and Timothy Ebner, Head of the Department of Neuroscience, will supervise operations along with a University-wide faculty steering committee to evaluate user needs, prioritize equipment acquisition and upgrades, recommend future staffing, identify funding opportunities, and promote cutting-edge research. Our goals will be to: Maximize the availability of advanced imaging technologies and methodologies to faculty, staff, students and external users. Provide expertise in new imaging technologies to users. Educate the University and broader community about new imaging technologies. Promote interaction between developers of new imaging technology and potential users. The first floor of Jackson Hall has been remodeled tohouse the Minneapolis facilities thanks to a $6.2 million NIH G20 grant secured by several departments and programs. The facility was also awarded a MN Parnership Grant with Drs. Ebner and Salisbury to install a multi-photon confocal microscope and a super-resolution micorscope at their respective facilities. The UIC has also been recognized as a Nikon Center of Excellence effective 12/17/12. The web sites have been consolidated and will now reside at http://www.uic.umn.edu.

    Impacts
    The UIC supports basic and multi-disciplinary applied research through microscopic imaging technologies aimed at evaluating options for improving plant and animal health and food safety. This project revision continues MAES support funding, and ensures researchers access to state-of-the-art equipment and staff in these research efforts. There have been over 700 research and instructional posters printed. There have been eight Graduate degrees that acknowledged the facility in 2012. Past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life-Fungus and NIH G20) relied on our facility. There were a multi-day workshops hosted by the UIC that trained participants on pioneering technical methodologies in sample preparation and fundimentals of imaging. Tours over one hundred K thru 12 students and teachers were hosted. New exhibits at the Science Museum of Minnesota are based on results from staff and collaborations. In the past year, 350 different projects from over seventy units (23 in St. Paul) have done research using the light and electron microscopy instrumentation totaling over 4700 hours. There were over 20 publications that acknowledged the Imaging Center. Usage is as follows: DEPTS OF College of Food, Agricultural and Natural ResourceSciences (1575+h): Ag. Engineering (96); Animal Science (90); Agronomy & Plant Genetics (200+); Entomology (270); Food Science & Nutrition (140); Plant Pathology (220). DEPTS OFCOLLEGE OF BIOLOGICAL SCIENCES (1300+h): Biotech. Inst. (360); Ecology Evo. Behav.(138); Genetics Cell Bio. Dev. (340); Imaging Center (140+); Plant Biology (425+). OTHER DEPARTMENTS (850+h): Chemistry (150+); Environ. Health (10); Lab Med & Pathology (60); Mech Eng (30); Pharmacology (250+); Vet Pathobiology (80); Vet Biomed (120+); Vet Clinical (60); NON-UNIVERSITY USERS (1100+h): American Petrographics, ATG Laboratories, Aveda, Biothera, Cargill, Creative Associates, HealthPartners Rsrch Foundation, Immunochemistry Technologies, LLC, Intelligent Nutrients, Invitrogen, Donaldson Inc., Life-Science Innovations, Land O'Lakes, Medtronic, Phillips Plastics, Receptor LLC, Schreiber Foods, Smiths Medical Md, Surmodics, WiXi AppTec. CLASSES & TOURS (130+h) INSTRUMENT CALIBRATIONS & MAINTENENCE (40.0). Additional activities include: 10+ invited presentations by UIC staff; local and natioinal microscopy meetings attendance; HCS Short course instructor at Marine Biological Laboratory at Woods Hole, MA. There have been 30+ publications that have utilized the UIC recourses. Below is a selection of publications associated with the UIC since the last submission.

    Publications

    • Sun Y, Lin Z, Reinders A, Ward JM. 2012. Functionally important amino acids in rice sucrose transporter OsSUT1 Biochemistry 51: 3284-3291.
    • Jonkers, W., Dong, Y., Broz, K., and Kistler, H.C. 2012. The Wor1-like protein Fgp1 regulates pathogenicity, toxin synthesis and reproduction in the phytopathogenic fungus Fusarium graminearum. PLoS Pathogens 8(5): e1002724.
    • Menke, J., Dong, Y., and Kistler, H.C. 2012. Fusarium graminearum Tri12p influences virulence to wheat and trichothecene accumulation. Molecular Plant-Microbe Interactions 25:1408-1418.
    • Berman, J and L. Hadany. 2012 Does stress induce (para)sex Implications for Candida albicans evolution. Trends in Genetics, 28: 1-7.
    • Burrack, L.S. and Berman, J. 2012. Flexibility of centromere and kinetochore structures. Trends in Genetics. 28:204-212.
    • Puvanendiran, S., and M.P. Murtaugh MP. 2011. Characterization of PCV2 growth in R1BL cells. LifeScience Alley 2011 Conference and Expo. Minneapolis, 8 Dec. Abst. 37. Robinson, S.R., C.R. Johnson, T.R. Griggs, J. Gnanandarajah, and M.P. Murtaugh. 2011. Characterization of the novel PRRSV ORF5a protein. XIIth International Nidovirus Symp. Abstract 004W, p 43.
    • Johnson, C., J. Li, and M.P. Murtaugh. 2011. Physical characterization of the porcine reproductive and respiratory syndrome virus virion. XIIth International Nidovirus Symp. Abstract 069P, p 71.
    • Lockhart, B., and Mollov, D. 2012. First report of Alfalfa mosaic virus occurrence in Tecoma capensis in the USA. Online. Plant Health Progress doi:10.1094/PHP-2012-08XX-01-BR.
    • Lockhart, B., Mollov, D., and Daughtrey, M. 2012. First report of Alfalfa mosaic virus occurrence in hydrangea in the USA. Online. Plant Disease doi: 10.1094/PDIS-07-12-0618-PDN.
    • Lockhart, B. 2012. First report of Turnip mosaic virus occurrence in garlic mustard in Minnesota. Online. Plant Health Progress doi:10.1094/PHP-2012-08XX-01-BR.
    • Lockhart, B., Mollov, D., and Voth-Hulshout, J. 2012. Association of spirea stunt phytoplasma with a disease of Spiraea spp. in Minnesota. Online. Plant Health Progress doi:10.1094/PHP-2012-1023-01-BR.
    • Mollov, D., Lockhart, B., Zlesak, D.C. 2012. Complete nucleotide sequence of rose yellow vein virus, a member of the family Caulimoviridae having a novel genome organization. Online. Archives of Virology doi:10.1007/s00705-012-1547-9.
    • Guaragna, M., Lamborn, J., Groth-Helms, D., Juszczak, S., Mollov, D., Lockhart, B., Schadewijk, T., Hammond, J., Jordan, R. 2012. First Report of Nerine yellow stripe virus in Amaryllis in the United States. Online. Plant Disease doi: 10.1094/PDIS-11-12-1042-PDN.
    • Whitfeld, T. J. S., D. L. Erickson, W. J. Kress, and G. D. Weiblen. 2012. Change in community phylogenetic structure during tropical forest succession: evidence from New Guinea. Ecography 34:1-10.
    • Whitfeld, T. J. S., V. Novotny, S. E. Miller, J. Hrcek, P. Klimes, and G. D. Weiblen. 2012. Predicting tropical insect herbivore abundance from host plant traits and phylogeny. Ecology 93:S211-S223.
    • Li, J. and M.P. Murtaugh. 2012. Dissociation of porcine reproductive and respiratory syndrome virus neutralization from antibodies specific to major envelope protein surface epitopes. Virology 433:367-376.
    • Murtaugh, M.P., C.R. Johnson, T.F. Griggs, S.R. Robinson, and J.S. Gnanandarajah. 2011. Novel structural protein in porcine reproductive and respiratory syndrome virus encoded in an alternative reading frame 5 present in all arteriviruses. Am Soc Virol 30th Ann Meet. Abstract W44-5, p 209.
    • Gora PJ, Reinders A, Ward JM (2012) A novel fluorescent assay for sucrose transporters. Plant Methods 8: 13.
    • Reinders A, Sun Y, Karvonen KL, Ward JM (2012) Identification of amino acids important for substrate specificity in sucrose transporters using gene shuffling. J Biol Chem 287 30296-30304.
    • Burrack, L.S. and Berman, J. 2012. Neocentromeres and epigenetically inherited features of centromeres. Chromosome Res. DOI 10.1007/s10577-012-9296-x.
    • Berman, J. 2012. Quick Guide: Candida albicans. Current Biology, 22(16):R620-22.
    • Anderson, M.Z, Baller, J.A., Dulmage, K., Wigen, L. and Berman, J. 2012. The three clades of the telomere-associated 1 TLO gene family of Candida albicans have different splicing, localization and expression features. Eukaryotic Cell, 11(10): 1268-1275.
    • Gerami-Nejad, Forche, A., McClellan, M., and Berman, J. 2012. Analysis of protein function in clinical Candida albicans isolates. Yeast. 29(8):303-309. PMID: 22777821; NIHMSID#390377.
    • Hickman, M.A, Zeng, G, Forche, A., Hirakawa,M.P., Abbey,D., Harrison, B.D., Wang, Y-M. Su, C-h., Bennett, R.J., Wang, Y and J Berman, 2012. The obligate diploid Candida albicans forms mating-competent haploids. Nature in press.
    • Choudhary S, Quin MB, Sanders MA, Johnson ET, Schmidt-Dannert C. 2012. Engineered protein nano-compartments for targeted enzyme localization. PLoS One. 2012;7(3):e33342. doi: 10.1371/journal.pone.0033342. Epub Mar 12. PMID: 22428024.
    • Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C. 2011. Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. Dec;92(6):1275-86. doi: 10.1007/s00253-011-3633-4. Epub 2011 Oct 28. PMID: 22033566.
    • Kumar, T. K. A., J. A. Crow, T. J. Wennblom, M. Abril, P. M. Letcher, M. Blackwell, R. W. Roberson, and D. J. McLaughlin. 2011. An ontology of fungal subcellular traits. Amer. J. Bot. 98: 1504-1510.
    • Kumar, T. K. A., R. Healy, J. W. Spatafora, M. Blackwell, and D. J. McLaughlin. Orbilia ultrastructure, character evolution and phylogeny of Pezizomycotina. Mycologia, publ. November 10, 2011 as doi:10.3852/11-213.


    Progress 01/01/11 to 12/31/11

    Outputs
    OUTPUTS: There are two main objectives for this project: 1) To support and maintain the core microscopy facilities for the benefit and enhancement of MAES sponsored agricultural research. 2) To provide scientific and technical guidance, assistance, and consultation on the application and use of light and electron optics and related technologies for the solution of both applied and fundamental research problems. Space does not permit an elaboration of the technical procedures used by these many and varied research efforts. Briefly, the resources of the Imaging Centers have been used in the development of new instruments and techniques; visualization of model systems including Arabidopisis, Medicago, Drosophila, yeast, C. elegans and Chlamydononas; discovery of new viruses and other microbes of plants and animals; the examination of cells and organelles in plant and animal tissue culture; biomedical device and reagent integration; experimental composition of food mixtures and dairy projects, elemental composition and morphology of soil particles and their importance in pollution-related research; the elemental composition of plants as related to practical problems in crop plant physiology and health; deterioration of textiles and forest products; various bioprocessing projects designed to lessen the environmental impact of paper and forest product production; renewable energy projects; nitrogen fixation of plants; alternatives to conventional insecticides; the adherence of pathogenic bacteria to food and food -processing surfaces; analysis of food storage materials; the identification and mapping of fungal chromosomes; and for the in situ identification of the activity of various genes of importance during genetic manipulations of vertebrate and plants. Since its inception, the Imaging Centers has served as the premier central facility for high-end imaging for the entire University community, serving over 450 users in the past fiscal year. Our target audience includes participation from K to 12 students and teachers; academic researchers at the university and college levels; corporate and industrial partners and governmental agencies. To make this multiple-user facility productive requires an environment of user cooperation, and guidelines for usage. General policy is provided by, Mark Sanders, Program Director, through an Advisory Committee and is reinforced through constant contact with project leaders and users. The Imaging Center is staffed by Dr. Gail Ceilo, Mr. Adam Vogel along with student workers Mr. Alex Cramer, Ms. Chemi Banari, and Ms. Ashleigh Skaalen. In addition to the AES funding, significant subsidy (~28%) toward the cost of operating is provided by the College of Biological Sciences. There are also user fees that are reviewed annually and posted on our website (www.cbs.umn.edu/ic). All fees subsidize operational costs. The facility is planned to operate under a cost neutral budget. PARTICIPANTS: Mark Sanders has been appointed the Program Director of the newly reorganized campus wide UIC. PARTICIPANTS: Mark Sanders, UIC Program Director. The facility is staffed by Dr. Gail Ceilo, Mr. Adam Vogel, Mr. Alex Cramer along with student workers, Ms. Chemi Banari, and Ms. Ashleigh Skaalen. TARGET AUDIENCES: Our target audience includes active usage by University researchers at the undergraduate, graduate, post doctoral and faculty levels. We also have significant participation from regional K-12 students and teachers; academic researchers at other universities and colleges; corporate and industrial partners and governmental agencies. We host workshops on sample preparation and image analysis. We presented in 2 national meetings. Mark Sanders is on the counsel of the Histochemical Society on the awards committee. PROJECT MODIFICATIONS: the College of Biological Sciences (CBS) and Academic Health Center/Medical School have agreed to merge the Biomedical Image Processing Lab (BIPL) and the Imaging Center (IC) to form the University Imaging Centers (UIC). The UIC will focus on meeting the imaging needs from across the campus, especially in vitro and live cell imaging, and provide related electron microscopic and computational technologies and expertise. Mark Sanders has been appointed program director. Michael O'Connor, head of the Department of Genetics, Cell Biology and Development, and Timothy Ebner, head of the Department of Neuroscience, will supervise operations. We are formed a University-Wide faculty steering committee to evaluate user needs, prioritize equipment acquisition and upgrades, recommend future staffing, identify funding opportunities, and promote cutting-edge research. Our goals will be to: Maximize the availability of advanced imaging technologies and methodologies to faculty, staff, students and external users. Bring expertise in new imaging technologies to users. Educate the University community about new imaging technologies. Promote interaction between developers of new imaging technology and potential users. The first floor of Jackson Hall has been remodeled to house the Minneapolis facilities thanks to a $3.4 million NIH G20 grant secured by several departments and programs. The web sites have been merged at http://www.uic.umn.edu.

    Impacts
    The Imaging Centers supports basic and multi-disciplinary applied research through microscopic imaging technologies aimed at evaluating options for improving plant and animal health and food safety. This project revision continues MAES support funding, and ensures researchers access to state-of-the-art equipment and staff in these research efforts. There have been over 600 research and instructional posters printed. There have been a dozen Masters or PhD degrees that acknowledged the facility in 2011. Past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life-Fungus) relied on our facility. There was a multi-day workshop hosted by the Center that trained participants on pioneering technical methodologies in sample preparation. Tours to dozens of K thru 12 students and teachers were well received. Exhibits at the Minnesota Zoo and the Science Museum of Minnesota are based on results from staff and collaborations. In the past year, 250 different projects from over a dozen units have done research using the light and electron microscopy instrumentation totaling over 4700 hours. There were over 20 publications that acknowledged the Imaging Center. Usage is as follows: DEPTS OF College of Food, Agricultural and Natural Resource Sciences (1575+h): Ag. Engineering (96); Animal Science (90); Agronomy & Plant Genetics (200+); Entomology (270); Food Science & Nutrition (140); Plant Pathology (220). DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES (1300+h): Biotech. Inst. (360); Ecology Evo. Behav. (138); Genetics Cell Bio. Dev. (340); Imaging Center (140+); Plant Biology (425+). OTHER UM DEPARTMENTS (600+h): Chemistry (150+); Environ. Health (10); Lab Med & Pathology (60); Mech Eng (30); Vet Pathobiology (80); Vet Biomed (120+); Vet Clinical (60); NON-UNIVERSITY USERS (800+h): American Petrographics, ATG Laboratories, Aveda, Biothera, Cargill, Creative Associates, HealthPartners Rsrch Foundation, Immunochemistry Technologies,LLC, Intelligent Nutrients, Invitrogen, Leica Microsystems, Life-Science Innovations, Land O'Lakes, Medtronic, Phillips Plastics, Receptor LLC, Schreiber Foods, Smiths Medical Md, Surmodics, WiXi AppTec. CLASSES & TOURS (130+h) INSTRUMENT CALIBRATIONS & MAINTENENCE (40.0). Multiple invited presentations by UIC staff. Attendance at local and natioinal microscopy meetings. Instructor at Marine Biological Laboratory at Woods Hole. There have been over 300 publications that have utilized the entire UIC recourses. Below is a selection of publications associated with the UIC since the last submission.

    Publications

    • Lockhart, B.E., and Mason, S.L. 2011. The potato corky ringspot pathogen, Tobacco Rattle Virus, occurs in native habitats in Minnesota. Plant Health Progress, doi 1094/PHP- 2011-1028-01-BR.
    • Lockhart, B., Zlezak, D and Fetzer, J. 2011. Identification and partial characterization off six new viruses of cultivated roses in the USA. Acta Hort. 901:139-148.
    • Olszewski, N.E. and Lockhart, B.E. 2011. Badnavirus: Caulimoviridae. In: The Springer Index of Viruses, 2nd Edition. C. Tidona and G. Darai (eds.), pp 263-269.
    • Subramanian, K., Park, Y., Verfaillie, C.M and Hu, W-S. 2011. Scalable expansion of multipotent adult progenitor cells as three-dimensional cell aggregates. Biotechnol. Bioeng.108, pp 364-375.
    • Hove, M. C., B. E. Sietman, J. E. Bakelaar, J. A. Bury, D. J. Heath, V. E. Pepi, J. E. Kurth, J. M. Davis, D. J. Hornbach, and A. R. Kapuscinski. 2011. Early life history and distribution of pistolgrip (Tritogonia verrucosa (Rafinesque, 1820)) in Minnesota and Wisconsin. American Midland Naturalist 165(2): 338-354.
    • Johnson, C.R., T.F. Griggs, J.S. Gnanandarajah, and M.P. Murtaugh. 2011. Novel structural protein in Porcine reproductive and respiratory syndrome virus encoded in an alternative open reading frame 5 present in all arteriviruses. Journal of General Virology. 92. Pp 1107-1116.
    • Loganathan G, Dawra RK, Pugazhenthi S, Guo Z, Soltani SM, Wiseman A, Sanders MA, Papas KK, Velayutham K, Saluja AK, Sutherland DE, Hering BJ, Balamurugan AN. Insulin Degradation by Acinar Cell Proteases Creates a Dysfunctional Environment for Human Islets Before/After Transplantation: Benefits of α-1 Antitrypsin Treatment. Transplantation. 2011 Dec 15;92(11):1222-1230.


    Progress 01/01/10 to 12/31/10

    Outputs
    OUTPUTS: The College of Biological Sciences Imaging Center a state-of-the-art core facility located on the St. Paul campus for imaging of biological specimens utilizing light microscopy; scanning and transmission electron microscopy; digital imaging analysis, visualization and printing; related to research, training and education. Since its inception, the Imaging Center has served as the premier central facility for high-end imaging for the University community, serving over 263 users in the past fiscal year. Briefly, the resources of the Imaging Center have been used in the development of new instruments and techniques; visualization of model systems including Arabidopisis, Medicago, Drosophila, yeast, C. elegans and Chlamydononas; discovery of new viruses and other microbes of plants and animals; the examination of cells and organelles in plant and animal tissue culture; biomedical device and reagent integration; experimental composition of food mixtures and dairy projects, elemental composition and morphology of soil particles and their importance in pollution-related research; the elemental composition of plants as related to practical problems in crop plant physiology and health; deterioration of textiles and forest products; various bioprocessing projects designed to lessen the environmental impact of paper and forest product production; renewable energy projects; nitrogen fixation of plants; alternatives to conventional insecticides; the adherence of pathogenic bacteria to food and food -processing surfaces; analysis of food storage materials; the identification and mapping of fungal chromosomes; and for the in situ identification of the activity of various genes of importance during genetic manipulations of vertebrate and plant cell lines. Our target audience includes participation from K-12 students and teachers; academic researchers at the university and college levels; corporate and industrial partners and governmental agencies. There have been over a dozen Masters or PhD degrees that acknowledged the facility in 2010. In the past year, 230 different projects from over a dozen units have done research using the light and electron microscopy instrumentation totaling over 4700 hours. There were over 50 publications that acknowledged the Imaging Center. To make this multiple-user facility productive requires an environment of user cooperation, and guidelines for usage. General policy is provided by, Mark Sanders, Program Director, through an Advisory Committee and is reinforced through constant contact with project leaders and users. The Imaging Center is staffed by Dr. Gail Ceilo, Mr. Adam Vogel and student workers Mr. Alex Cramer, Ms. Chemi Banari, Ms. Ashleigh Skaalen, and Ms. Alex Schaurer. In addition to the AES funding, significant subsidy (~28%) toward the cost of operating is provided by the College of Biological Sciences. There are also user fees that are reviewed annually and posted on our website (www.cbs.umn.edu/ic). All fees subsidize operational costs. The facility is planned to operate under a cost neutral budget for the past 6 years. PARTICIPANTS: Mark Sanders has been appointed the Program Director of the newly re-organized UIC. In the past year, 230 different projects (263 indivdual accounts) from over a dozen units (14) have done research using the light and electron microscopy instrumentation totaling over 4700 hours.DEPTS OF College of Food, Agricultural and Natural Resource Sciences (1553h): Ag. Engineering (90); Animal Science (80); Agronomy & Plant Genetics (200); Entomology (350); Food Science & Nutrition (160); Plant Pathology (200). DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES (1249h): Biotech. Inst. (351); Ecology Evo. Behav. (185); Genetics Cell Bio. Dev. (321); Imaging Center (160); Plant Biology (405). OTHER UM DEPARTMENTS (506h): Chemistry (151); Environ. Health (10); Fisheries Wildlife Cons Bio (63); Lab Med & Pathology (65); Mech Eng (9); Oral Sciences (8); Vet Pathobiology (73); Vet Biomed (102); Vet Clinical (28); Vet Population (6). NON-UNIVERSITY USERS (892h): American Petrographics (10), ATG Laboratories (5), Aveda (65), Biothera (17), Cargill (90), Creative Associates (16), HealthPartners Rsrch Foundation (14), Immunochemistry Technologies,LLC (20), Intelligent Nutrients (12), Invitrogen (40), Life-Science Innovations (8), Land O'Lakes (35), , Medtronic (200), NIOSH/CDC Spokane (31), Phillips Plastics (8), Receptor LLC (20), Schreiber Foods (80), Smiths Medical Md (42), Surmodics (18), WiXi AppTec (124). CLASSES & TOURS (130) INSTRUMENT CALIBRATIONS & MAINTENENCE (40.0) TARGET AUDIENCES: Our target audience includes active usage by University researchers at the undergraduate, graduate, post doctoral and faculty levels. We also have significant participation from K-12 students and teachers; academic researchers at other universities and colleges; corporate and industrial partners and governmental agencies. PROJECT MODIFICATIONS: To meet the growing biomedical imaging needs of the University research community, the College of Biological Sciences (CBS) and Academic Health Center/Medical School have merged the Biomedical Image Processing Lab (BIPL) and the Imaging Center (IC). The new entity, the University Imaging Centers (UIC), will focus on meeting the imaging needs from across the campus, especially in vitro and live cell imaging, and provide related electron microscopic and computational technologies and expertise. Mark Sanders has been appointed program director. Michael O'Connor, head of the Department of Genetics, Cell Biology and Development, and Timothy Ebner, head of the Department of Neuroscience, will supervise operations. We are formed a University-Wide faculty steering committee to evaluate user needs, prioritize equipment acquisition and upgrades, recommend future staffing, identify funding opportunities, and promote cutting-edge research. Our goals will be to: Maximize the availability of advanced imaging technologies and methodologies to faculty, staff, students and external users. Bring expertise in new imaging technologies to users. Educate the University community about new imaging technologies. Promote interaction between developers of new imaging technology and potential users. The first floor of Jackson Hall is being remodeled to house the Minneapolis facilities thanks to a $3.4 million NIH G20 grant secured by several departments and programs. Meanwhile, services will continue uninterrupted in their current locations during the same hours and we will begin to expand services at both locations. Please see the Imaging Center web site at http://www.cbs.umn.edu/ic/ or the BIPL web site at http://bipl.umn.edu/ for information. The Web sites will be merged under the new name in the near future at http://uic.umn.edu.

    Impacts
    The Imaging Center supports basic and multi-disciplinary applied research through microscopic imaging technologies aimed at evaluating options for improving animal health and food safety. This project revision continues MAES support funding, and ensures researchers access to state-of-the-art equipment and staff in these research efforts. There have been over 2500 research and instructional posters printed. Past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life- Fungus) relied on our facility. There were two multi-day workshops that were sponsored by the Center that trained 30+ users on pioneering technical methodologies in sample preparation. Tours to dozens of K-12 students and teachers were well received. Exhibits at the Minnesota Zoo and the Science Museum of Minnesota are based on results from staff and collaborations. Usage is as follows: DEPTS OF College of Food, Agricultural and Natural Resource Sciences (1553h): Ag. Engineering (90); Animal Science (80); Agronomy & Plant Genetics (200); Entomology (350); Food Science & Nutrition (160); Plant Pathology (200). DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES (1249h): Biotech. Inst. (351); Ecology Evo. Behav. (185); Genetics Cell Bio. Dev. (321); Imaging Center (160); Plant Biology (405). OTHER UM DEPARTMENTS (506h): Chemistry (151); Environ. Health (10); Fisheries Wildlife Cons Bio (63); Lab Med & Pathology (65); Mech Eng (9); Oral Sciences (8); Vet Pathobiology (73); Vet Biomed (102); Vet Clinical (28); Vet Population (6). NON-UNIVERSITY USERS (892h): American Petrographics (10), ATG Laboratories (5), Aveda (65), Biothera (17), Cargill (90), Creative Associates (16), HealthPartners Rsrch Foundation (14), Immunochemistry Technologies,LLC (20), Intelligent Nutrients (12), Invitrogen (40), Life-Science Innovations (8), Land O'Lakes (35), , Medtronic (200), NIOSH/CDC Spokane (31), Phillips Plastics (8), Receptor LLC (20), Schreiber Foods (80), Smiths Medical Md (42), Surmodics (18), WiXi AppTec (124). CLASSES & TOURS (130) INSTRUMENT CALIBRATIONS & MAINTENENCE (40.0)25+ of the key publications are listed below.

    Publications

    • B. E. L. Lockhart, S. L. Mason, D. A. Johnson, and D. S. Mollov. First Report of Tobacco etch virus Infection in Coleus in the United States. Plant Disease Jul 2010, Volume 94, Number 7: 921.
    • B. E. Lockhart and S. L. Mason. First Report of Tobacco rattle virus in Sedum in Minnesota. Plant Disease Mar 2010, Volume 94, Number 3: 374.
    • Bower, R., K. VanderWaal, E. OʼToole, L. Fox, C. Perrone, J. Mueller, M. Wirschell, R. Kamiya, S. Goldstein, W.S. Sale, and M.E. Porter (2009) IC138 defines a sub-domain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol. Biol. Cell 20:3055-3063.
    • Chengala AA, Hondzo M, Troolin D, Lefebvre PA. Kinetic responses of Dunaliella in moving fluids. Biotechnol Bioeng. 2010 Sep 1;107(1):65-75. PubMed PMID: 20506336.
    • Craft, K. J., S. Pauls, K. Darrow, P. D. N. Hebert, L. Helgen, S. E. Miller, V. Novotny, and G. D. Weiblen. 2010. Population genetics of ecological communities with DNA barcodes: An example from New Guinea Lepidoptera. Proceedings of the National Academy of Sciences of the United States of America 107:5041-5046.
    • Healy, R., T.K. Kumar, and D. J. McLaughlin. Phylogenetic implications of septal pore ultrastructure in the basal ascomycete Neolecta irregularis. Botany & Mycology 2009, 65, 2009.
    • Helge Jochens, Konstanze Stiba, Christopher Savile, Ryota Fujii, Juin-Guo Yu, Tatsiana Gerassenkov, Romas J. Kazlauskas, Uwe T. Bornscheuer. Converting an esterase into an epoxide hydrolase. Angew. Chem. Intl. Ed. 2009, 48, 3532-3535. doi: 10.1002/anie.200806276.
    • Hertzel AV, Hellberg K, Reynolds JM, Kruse AC, Juhlmann BE, Smith AJ, Sanders MA, Ohlendorf DH, Suttles J, Bernlohr DA. Identification and characterization of a small molecule inhibitor of Fatty Acid binding proteins. J Med Chem. 2009 Oct 8;52(19):6024-31.
    • Heuser, T., M. Raytchev, J. Krell, M.E. Porter, and D. Nicastro (2009). The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella. J. Cell Biol. 187:921-933.
    • Jae-Hoon Park, Hyun-Joon Ha, Won Koo Lee, Tobie Genereux-Vincent, Romas J. Kazlauskas Molecular basis for the stereoselective ammoniolysis of N-alkyl aziridine- 2-carboxylates catalyzed by Candida antarctica lipase B. ChemBioChem, 2009, 10, 2213-2222. doi: 10.1002/cbic.200900343
    • Lockhart, B., Olzewski, N., and Mason, S. 2009. Genomic characterization of a seed-borne caulimovirus associated with flower distortion in Rudbeckia hirta. Phytopathology 99:576.
    • Machado-Caballero, J.E., Lockhart, B.E.L., Mason, S.L., and Daughtry, M. 2009. Identification and properties of a caulimovirus causing chlorotic mottle of florists' hydrangea (Hydrangea macrophylla) in the United States. Plant Dis. 93:891-895.
    • McLaughlin,D. J., D. S. Hibbett, F. Lutzoni, J. W. Spatafora, and R. Vilgalys. The search for the Fungal Tree of Life. Trends Microbiology. Volume #: 17: 488-497, 2009.
    • Mollov, D., Lockhart, B., and Zlesak, D. 2009. Genome characterization and transmission of Rose yellow vein virus, a new caulimovirus occurring in garden rose. Phytopathology 99:587.
    • Piasecki BP, Silflow CD. The UNI1 and UNI2 genes function in the transition of triplet to doublet microtubules between the centriole and cilium in Chlamydomonas. Mol Biol Cell. 2009 Jan;20(1):368-78. Epub 2008 Nov 12. PubMed PMID: 19005206; PubMed Central PMCID: PMC2613092.
    • Qi Y, Tsuda K, Joe A, Sato M, Nguyen le V, Glazebrook J, Alfano JR, Cohen JD, Katagiri F. A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis. Mol Plant Microbe Interact. 2010 Dec;23(12):1573-83.
    • Qing Jing, Krzysztof Okrasa, Romas J. Kazlauskas. Stereoselective hydrogenation of olefins using rhodium-substituted carbonic anhydrase a new reductase. Chem. Eur. J. 2009, 15, 1370-1376. http://dx.doi.org/10.1002/chem.200801673.
    • Romas J. Kazlauskas, Uwe T. Bornscheuer, Finding better protein engineering strategies. Nature Chem. Biol. 2009, 5, 526-529. doi: 10.1038/nchembio0809-526
    • Schilling, J.S. (2010) Effects of calcium-based materials and iron impurities on wood degradation by the brown rot fungus Serpula lacrymans. Holzforschung 64: 93-99.
    • Shona Duncan, Qing Jing, Adrian Katona, Romas J. Kazlauskas, Jonathan Schilling, Ulrike Tschirner, Waleed Wafa AlDajani. Increased saccharification yields from aspen biomass upon treatment with enzymatically generated peracetic acid. Appl. Biochem. Biotechnol. 2009, online. doi: 10.1007/s12010-009-8639-3
    • Staginnus, C., Iskra-Caruana, M.L., Lockhart, B., Hohn, T., and Richert-Poggeler, K.R. 2009. Suggestions for a nomenclature of endogenous pararetroviral sequences in plants. Archives of Virology 154:1189-1193.
    • Starich, T.A., Xu, J., Skerrett, I.M., Nicholson, B. J., Shaw, J.E. Interactions between innexins UNC-7 and UNC-9 mediate electrical synapse specificity in the C. elegans locomotory nervous system. Neural Development. Volume #:4, 16, 2009.
    • Tian H, Baxter IR, Lahner B, Reinders A, Salt DE, Ward JM. Arabidopsis NPCC6/NaKR1 Is a Phloem Mobile Metal Binding Protein Necessary for Phloem Function and Root Meristem Maintenance. Plant Cell. 2010 Dec 30. [Epub ahead of print] PubMed PMID: 21193571.
    • Whitfeld, T. J. S. and G. D. Weiblen. 2010. Five New Ficus Species (Moraceae) from Melanesia. Harvard Papers in Botany 15(1):1-10
    • Wirschell, M., C. Yang, P. Yang, L. Fox, H. Yanigasawa, R. Kamiya, G.B. Witman, M.E. Porter, and W.S. Sale (2009) IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet sliding. Mol. Biol Cell 20:3044-3054.


    Progress 01/01/09 to 12/31/09

    Outputs
    OUTPUTS: The College of Biological Sciences Imaging Center a state-of-the-art core facility located on the St. Paul campus for imaging of biological specimens utilizing light microscopy; scanning and transmission electron microscopy; digital imaging analysis, visualization and printing; related to research, training and education. In the 15 years since its inception, the Imaging Center has served as the premier central facility for high-end imaging for the University community, serving over 250 users in the past fiscal year. Briefly, the instrumentation and resources of the Imaging Center have been used in the development of new instruments and techniques; visualization of model systems including Arabidopisis, Medicago, Drosophila, yeast, C. elegans and Chlamydononas; discovery of new viruses and other microbes of plants and animals; the examination of cells and organelles in plant and animal tissue culture; biomedical device and reagent integration; experimental composition of food mixtures and dairy projects, elemental composition and morphology of soil particles and their importance in pollution-related research; the elemental composition of plants as related to practical problems in crop plant physiology and health; deterioration of textiles and forest products; various bioprocessing projects designed to lessen the environmental impact of paper and forest product production; renewable energy projects; nitrogen fixation of plants; alternatives to conventional insecticides; the adherence of pathogenic bacteria to food and food -processing surfaces; analysis of food storage materials; the identification and mapping of fungal chromosomes; and for the in situ identification of the activity of various genes of importance during genetic manipulations of vertebrate and plant cell lines. There have been over a dozen Masters or PhD degrees that acknowledged the facility in 2009. In the past year, 226 different projects from over a dozen units have done research using the light and electron microscopy instrumentation totaling 4360 hours. There were over 60 publications that acknowledged the Imaging Center. The Imaging Center is staffed by Dr. Gail Ceilo, Mr. Tracy Anderson and student workers Mr. Alex Cramer, Ms. Chemi Banari, Ms. Ashleigh Skaalen, Ms. Alex Schaurer and Ms. Anna Hottinger. In addition to the AES funding, significant subsidy (~28%) toward the cost of operating is provided by the College of Biological Sciences. There are also user fees that are reviewed annually and posted on our website (www.cbs.umn.edu/ic). All fees subsidize operational costs. The facility has been managed to operate under a cost neutral budget for the past 6 years. Our target audience includes participation from K-12 students and teachers; academic researchers at the university and college levels; corporate and industrial partners and governmental agencies. Usage is as follows: DEPTS OF College of Food, Agricultural and Natural Resource Sciences (1516h): DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES (1199h): OTHER UM DEPARTMENTS (476h) NON-UNIVERSITY USERS (712h CLASSES & TOURS (90) INSTRUMENT CALIBRATIONS & MAINTENENCE (40.0) PARTICIPANTS: To make this multiple-user facility productive requires an environment of user cooperation, and guidelines for usage. General policy is provided by the Program Director through an Advisory Committee and is reinforced through constant contact with project leaders and users. MAES project leaders, their technical personnel, and research students have priority on equipment usage and on the assistance consultation provided by Mark Sanders. Since 1990 MAES users have been charged subsidized user fees so that accessibility to agricultural researchers is maintained. Other University of Minnesota research programs and industrial users have second priority to equipment usage. All fees subsidize operational costs. TARGET AUDIENCES: Our target audience includes participation from K-12 students and teachers; academic researchers at the university and college levels; corporate and industrial partners and governmental agencies. PROJECT MODIFICATIONS: After 32 years of service at the University of Minnesota, Mr. Gilbert Ahlstrand retired this year. His duties as PI have been taken over by Mark Sanders

    Impacts
    The Imaging Center supports basic and multi-disciplinary applied research through microscopic imaging technologies aimed at evaluating options for improving animal health and food safety. This project revision continues MAES support funding, and ensures researchers access to state-of-the-art equipment and staff in these research efforts. There have been over 2100 research and instructional posters printed. Past and current grants (NSF Cytoskeletal Training Grant, NSF Tree of Life- Fungus) relied on our facility. There were two multi-day workshops that were sponsored by the Center that trained 28 users on pioneering technical methodologies. Tours to dozens of K-12 students and teachers were well received. Exhibits at the Minnesota Zoo and the Science Museum of Minnesota are based on results from staff and collaborations.

    Publications

    • ODougherty M, Smith AJ, Kurzer M, Schmitz KH. (2009) Theory in practice: Young womens reflections on motivations for physical activity over time and across contexts. International Society of Behavioral Nutrition and Physical Activity, Lisbon, Portugal, June, 2009.
    • Orozco-Velasco, F., V. Aldana-Martinez, C. Franco-Ramos, C. Rosales-Jimenez, M. Vazquez-Pichardo, and F. Diez-Gonzalez. 2009. Improvement in dengue fever diagnostics in the Mexican state of Jalisco by a new protocol based on the detection of the viral NS1 antigen and the IgM and IgG antibodies. ASM Annual Meeting. May 17-21, Philadelphia, PA.
    • Orr LR, Redmon JB, Kurzer MS, Raatz SK. (2009) Effects of Dietary Fat and Omega-3 Fatty Acid Intake on Plasma Fatty Acids and Hormone Levels in Post Menopausal Women. 2009 AICR Annual Research Conference on Food, Nutrition, Physical Activity & Cancer, Washington, D.C.
    • Qiuqiong Huang, 2009, Chapter 5. Management of Irrigation Water in Rural China, in John Briscoe et al., Water and Agriculture: Implications for Development and Growth (Washington, D.C.: Center for Strategic and International Studies, 2009).
    • Qiuqiong Huang, Scott Rozelle, Jikun Huang, Jinxia Wang and Siwa Msangi, 2008, Water management reform and the choice of the contractual form in rural China. Environment and Development Economics 13(2): 171-200.
    • Qiuqiong Huang, Scott Rozelle, Jixia Wang and Jikun Huang, 2008, Water Management Institutional Reform: A Representative Look at Northern China, Agricultural Water Management, 96(2): 215-225.
    • Samavat H, Arikawa AY, Espejo LA, Kurzer M. (2009) Association of abdominal adiposity and circulating appetite hormones in healthy premenopausal women. Experimental Biology, New Orleans, LA. Sapiro JM, Mashek MT, Greenberg AS, *Mashek DG*. Hepatic triacylglycerol hydrolysis activates PPAR-a. /Journal of Lipid Research /2009;50(8):1621-1629/./ PMID: 19304987.
    • Smith A, Arikawa A, Schmitz K, Kurzer M. (2009) Aerobic Exercise, Estrogens and Breast Cancer Risk in Premenopausal Women. 9^th annual meeting of the American College of Sports Medicine, Seattle, WA.
    • Smith AJ, Arikawa A, ODoughertyM, Kaufman B, Jakits H, Schmitz KH, Kurzer M. (2009) Is Body Mass Index (BMI) A Good Indicator of Health and Healthy Behavior in Young, Sedentary Women International Society of Behavioral Nutrition and Physical Activity, Lisbon, Portugal, June, 2009.
    • Thompson, B.R., Mazurkiewicz-Munoz, A.M., Suttles, J., Carter-Su, C., and Bernlohr, D.A. (2009) Interaction of adipocyte fatty acid binding protein and Jak2; AFABP/aP2 as a regulator of Jak2 signaling. J. Biol. Chem., 284: 13473-13480. PMID: 19318353
    • Viazis, S., M. Akhtar, J. Feirtag, and F. Diez-Gonzalez. 2009. Isolation and characterization of lytic bacteriophages against enterohemorrhagic /Escherichia coli. /IAFP Annual Meeting, July 12-15, Dallas, TX.
    • Wang, N., F. Diez-Gonzalez, T. P. Labuza and T. Blasius. 2009. Thermal and chemical inactivation of ricin in orange juice. IAFP Annual Meeting, July 12-15, Dallas, TX.
    • Wiczer, B.M., and Bernlohr, D.A. (2009) A Novel Role for FATP1 in the Regulation of TCA Cycle and Mitochondrial Function in 3T3-L1 Adipocytes. J. Lipid Res., 50; 2502-2513 PMID: 19535819
    • Willis HJ, Thomas W, Eldridge AI, Harkness L, Green H, Slavin JL (2009) Dietary fiber does not influence satiety, glucose, and insulin in a dose-dependent manner. FASEB J (abstract 545.5).
    • Raatz SR, Orr LR, Redmon JB, Kurzer MS. (2009) Effect of dietary fat and fatty acids on plasma estrogen and testosterone indices in postmenopausal women suggesting a reduction in breast cancer risk. Annual meeting of the American Dietetic Association, FNCE 2009, Denver CO, October 2009.
    • Rebello, SA, Slaton JW, Anderson LB, Kuskowski MA, Hamilton-Reeves JM, Kurzer M. (2009) Effects of soy protein on prostate tissue protein expression in men at high risk of prostate cancer - a proteomics approach. American Urological Association Annual Meeting, Chicago, Illinois, April, 2009.
    • Resnick TD, Dej KJ, Xiang Y, Hawley RS, Ahn C, Orr-Weaver TL. Mutations in the chromosomal passenger complex and the condensin complex differentially affect synaptonemal complex disassembly and metaphase I configuration in Drosophila female meiosis. Genetics. 2009. Mar; 181(3):875-87.
    • Richards, V., Godfrey, I., Blanchette, R., Held, B., Gregory, D. & Reed, E. (2009), In-Situ Monitoring and Stabilisation of the James Matthews Site in Proceedings of the 10th ICOM Group on Wet Organic Archaeological Materials Conference, Amsterdam, 10-15 September 2007, eds K. Straetkvern & D.J. Huisman, Rijksdienst voor Archeologie, Cultuurlandschap en Monumenten (RACM), Amersfoort, pp. 113-160.
    • Willis HJ, Thomas W, Eldridge AL, Harkness L, Green H, Slavin JL. (2009) Effect of different doses of dietary fiber on satiety response and food intake. European Association for the Study of Obesity, Obesity Facts 2(supple2):142.
    • Wu, X., J. Zhu, C. Dong, C. Miller, Y. Li, L. Wang, W. Yao. Title: Continuous biohydrogen production from liquid swine manure supplemented with glucose using an anaerobic sequencing batch reactor. International Journal of Hydrogen Energy. 34(16), 6636-6645. 2009.
    • Zhou Y., and Ni, M. (2009). SHB1 plays dual roles in photoperiodic and autonomous flowering. Developmental Biology 331, 50-57.
    • Zhou Y., Zhang, X., Kang, X., Zhao, X., Zhang, X., and Ni, M. (2009). SHORT HYPOCOTYL UNDER BLUE1 associates with MINISEED3 and HAIKU2 promoters in vivo to control Arabidopsis seed development. Plant Cell 21, 106-117.
    • Bu SY, Mashek MT, Mashek DG. Suppression of long chain acyl-CoA synthetase (ACSL3) decreases hepatic de novo fatty acid synthesis through decreased transcriptional activity. /Journal of Biological Chemistry 2009;284(44):30474-30483./ PMID: 19737935.
    • Adair, E. C., P. B. Reich, S. E. Hobbie, and J. M. H. Knops. 2009. Interactive effects of time, CO2, N and diversity on total belowground carbon allocation and ecosystem carbon storage in a grassland community. Ecosystems 12:1037-1052
    • Adair, E. C., S. E. Hobbie, R. K. Hobbie. 2010. Single pool exponential decomposition models: potential pitfalls in their use in ecological studies. Ecology
    • Akhtar, M., S. Viazis, J. Feirtag, and F. Diez-Gonzalez. 2009. Isolation and identification of bacteriophages against /Salmonella/ Typhimurium IAFP Annual Meeting, July 12-15, Dallas, TX.
    • Alekel^ DL, Van Loan^ MD, Koehler^ KJ, Hanson^ LN, Stewart JW, Hanson KB, Kurzer^ MS, Peterson^ CT. (2009) Soy Isoflavones for Reducing Bone Loss (SIRBL) Study: Randomized three year intervention in postmenopausal women to determine efficacy and safety. 31^st Annual meeting of the American Society for Bone and Mineral Research, Denver, CO.
    • Bryan Lohmar, Qiuqiong Huang, Bo Lei, and Zhanyi Gao, 2008, Water Pricing Policies and Recent Reforms in China: the Conflict Between Conservation and Other Policy Goals in Irrigation Water Pricing The Gap Between Theory and Practice eds. Francois Molle and Jeremy Berkoff, Publisher: CAB International, ISBN13: 978-1-84593-292-3.
    • Cheng, H., Govindan, J. A., & Greenstein, D. (2008). Regulated trafficking of the MSP/Eph receptor during oocyte meiotic maturation in C. elegans. Current Biology 18: 705-714.
    • Clark, C. M., S. E. Hobbie, R. Venterea, and D. Tilman. 2009. Long-lasting effects on N cycling 12 years after treatments cease despite minimal N retention. Global Change Biology 15:1755-1766
    • Clement, W. L., and G. D. Weiblen 2009. Morphological evolution in the mulberry family (Moraceae). Systematic Botany 34: 530-552.
    • Dan Zhu, Amy Tran, Heather M. Teterka, Theodore P. Labuza. Effect of cysteine on lowering protein aggregation and subsequent hardening of WPI protein bars. IFT Annual Meeting 2009 Anaheim, CA. PMID: 09-A-3192-IFT.
    • Arenz, B. E. and R. A. Blanchette. 2009. Investigations of fungal diversity in wooden structures and soils at historic sites on the Antarctic Peninsula. Canadian Journal of Microbiology 55:46-56.
    • Bentivenga, S.P., T.K.A. Kumar, L. Kumar, G.J. Celio, R.W. Roberson, and D.J. McLaughlin. 2009. Cellular organization in germ tube tips of the Glomeromycota: the taming of Gigaspora. Inoculum: Newsletter of the Mycological Society of America (in press).
    • de Boer, J. G., P. J. Ode, A. K. Rendahl, L. E. M. Vet, J. Whitfield, and G. E. Heimpel. 2008. Experimental support for multiple-locus complementary sex determination in the parasitoid Cotesia vestalis. Genetics 180:1525-1535.
    • Bentivenga, S.P., T.K.A. Kumar, L. Kumar, G.J. Celio, R.W. Roberson, and D.J. McLaughlin. 2009. Cellular organization in germ tube tips of Gigaspora. Abstracts of the 6th International Conference on Mycorrhiza, Belo Horizonte, Brazil, pg 34.
    • BENTIVENGA, STEPHEN P., KUMAR, T.K. ARUN, KUMAR, LETICIA, CELIO, GAIL J., ROBERSON, ROBERT W. and MCLAUGHLIN, DAVID J. 2009. Cellular organization in germ tube tips of the Glomeromycota: the taming of Gigaspora. Inoculum. Volume #: 60(3). Page #'s: 10.
    • Brian P. Piasecki and Carolyn D. Silflow. 2009. The UNI1 and UNI2 genes function in the transition of triplet to doublet microtubules between the centriole and cilium in Chlamydomonas. Molecular Biology of the Cell. Vol. 20 368-378.
    • de Boer, J. G., P. J. Ode, L. E. M. Vet, J. Whitfield, and G. E. Heimpel. 2007. Diploid males sire triploid daughters and sons in the parasitoid wasp Cotesia vestalis. Heredity 99:288-294.
    • de Boer, J. G., P. J. Ode, L. E. M. Vet, J. Whitfield, and G. E. Heimpel. 2007. Complementary sex determination in the parasitoid wasp Cotesia vestalis (= C. plutellae). Journal of Evolutionary Biology 20:340-348. Diez-Gonzalez, F., M. B. Kamnetz and Z. M. Vickers, 2009. Use of nisin and caprylic acid to control /Listeria monocytogenes/ in queso fresco. IAFP Annual Meeting, July 12-15, Dallas, TX.
    • Dijkstra, F. A., J. B. West, S. E. Hobbie, and P. B. Reich. 2010. Antagonistic effects of species on C respiration and net N mineralization in soils from mixed coniferous plantations. Forest Ecology and Management Ferris AM, Giberson RT, Sanders MA, Day JR. Advanced laboratory techniques for sample processing and immunolabeling using microwave radiation. Journal of Neuroscience Methods. Available online 9 June 2009, ISSN 0165-0270, DOI: 10.1016/j.jneumeth.2009.06.002.
    • Fissore, C., J. Espeleta, E. A. Nater, S. E. Hobbie, and P. B. Reich. 2010. Limited potential for terrestrial carbon sequestration to offset fossil fuel emissions in the Upper Midwest of the U. S. Frontiers in Ecology and the Environment.
    • Gallaher, C.M. and Gallaher, D.D. 2009. Dried plums (prunes) reduce atherosclerosis lesion area in apolipoprotein E-deficient mice. British J. Nutrition. . Vol. 101, 233-239. Govindan, J. A., Nadarajan, S., Kim, S., Starich, T. A., & Greenstein, D. (2009). Somatic cAMP signaling regulates MSP-dependent oocyte growth and meiotic maturation in C. elegans. Development 136: 2211-2221.
    • HEALY, ROSANNE, KUMAR, T.K. ARUN and MCLAUGHLIN, DAVID J. 2009. Phylogenetic implications of septal pore ultrastructure in the basal Ascomycete Neolecta irregularis. Inoculum. Volume #: 60(3).
    • Hertzel, A.V., Hellberg, K., Reynolds, J.M., Kruse, A.C., Juhlmann, B.E., Smith, A.J., Sanders, M.A., Ohlendorf, D.H., Suttles, J. and Bernlohr, D.A. 2009. Identification and Characterization of a Small Molecule Inhibitor of Fatty Acid Binding Protein, J. Med. Chem. 52, 6024-6031 PMID: 19754198
    • Janet B. Rollefson, Caleb E. Levar, Daniel R. Bond. Identification of Genes Involved in Biofilm Formation and Respiration via Mini-Himar Transposon Mutagenesis of Geobacter sulfurreducens. Journal of Bacteriology, 2009, 191. 4207-4217.
    • Jinxia Wang, J. Huang, Qiuqiong Huang, S. Rozelle and H.F. Farnsworth, 2009, The evolution of groundwater governance: productivity, equity and changes in the level of China's aquifers Quarterly Journal of Engineering Geology and Hydrogeology, 42: 267-280.
    • Jinxia Wang, Jikun Huang, Scott Rozelle, Qiuqiong Huang and Lijuan Zhang, 2009, Understanding the Water Crisis in Northern China: What Government and Farmers are Doing. International Journal of Water Resources Development, 25(1): 141 - 158.
    • Hobbie, S. E., J. Oleksyn, D. M. Eissenstat, and P. B. Reich. 2010. Fine root decomposition rates do not mirror those of leaf litter among temperate tree species. Oecologia
    • Jurgens, J. A., R. A. Blanchette and T. R. Filley. 2009. Fungal diversity and deterioration in mummified woods from the ad Astra Ice Cap region in the Canadian High Arctic. Polar Biology 32:751-758.
    • Kaufman B, Thieschafer A, Wetzsteon R, Stovitz S, Petit M. (2009) Tibial and radial bone strength in women is predicted by muscle load and sex steroids. 9^th annual meeting of the American College of Sports Medicine, Seattle, WA.
    • Keeler, B. L., S. E. Hobbie, and L. Kellogg. 2009. Effects of long-term nitrogen additions on soil and litter microbial enzyme activity in eight forested and grassland sites - implications for litter and SOM decomposition. Ecosystems 12:1-15
    • Kren B, Unger G, Trossen A, Korman V, Diethelm-Okita B, Reding M, Steer C (2009) Nanoencapsulated Sleeping Beauty mediates therapeutic Factor VIII expression in liver sinusoidal endothelial cells of hemophilia A mice, J Clin Invest Jul:119(7):2086-99.
    • Kuriyama R, Bettencourt-Dias M, Hoffmann I, Arnold M, Sandvig L. 2009. Tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells. J Cell Sci. 122, 2014-2023. Kuriyama, R. 2009. Centriole assembly in CHO cells expressing Plk4/SAS6/SAS4 is similar to centriogenesis in ciliated epithelial cells. Cell Motil. Cytoskeleton 66, 588-596.
    • Lobo, S., Wiczer, B. M., and Bernlohr, D.A. (2009) Functional analysis of long chain acyl CoA synthetase in 3T3-L1 adipocytes. J. Biol. Chem, 284; 18347-18356 PMID: 19429676
    • Marks, M. D., L. Tian, J. P. Wenger, S. N. Omburo, W. Soto-Fuentes, H. Ji, D. Gang, G. D. Weiblen, and R. A. Dixon 2009. Identification of candidate genes affecting delta-9-tetrahydrocannabinol biosynthesis in Cannabis sativa. Journal of Experimental Botany 13: 3175-3726.
    • MD Marks, JP Wenger, E Gilding, R Jilk, and RA Dixon. Transcriptome analysis of Arabidopsis wild-type and gl3-sst sim trichomes identifies four additional genes required for trichome development. 2009. Molecular Plant Volume #: 2, 803-822.
    • Nadarajan, S., Govindan, J. A., McGovern, M., Hubbard, E. J., & Greenstein, D. (2009). MSP and GLP-1/Notch signaling coordinately regulate actomyosine-dependent cytoplasmic streaming and oocyte growth in C. elegans. Development 136: 2223-2234.


    Progress 01/01/08 to 12/31/08

    Outputs
    OUTPUTS: We are a state-of-the-art core facility for imaging of biological specimens utilizing light microscopy; scanning and transmission electron microscopy; digital imaging and printing; related to research, training and education. Briefly, the instrumentation and resources of the Imaging Center have been used in the development of new instruments and techniques; discovery new viruses and other microbes of plants and animals; the examination of cells and organelles in plant and animal tissue culture; biomedical device and reagent integration; experimental composition of food mixtures and dairy projects, elemental composition and morphology of soil particles and their importance in pollution-related research; the elemental composition of plants as related to practical problems in crop plant physiology and health; deterioration of textiles and forest products; various bioprocessing projects designed to lessen the environmental impact of paper and forest product production; renewable energy projects; nitrogen fixation of plants; alternatives to conventional insecticides; the adherence of pathogenic bacteria to food and food processing surfaces; analysis of food storage materials; the identification and mapping of fungal chromosomes; and for the in situ identification of the activity of various genes of importance during genetic engineering of plants. In the past reporting period, 226 different projects have done research using the light and electron microscopy instrumentation totaling 3360 hours. There have been over 2000 research and instructional posters printed. There were 5 Masters or PhD degrees that acknowledged the facility. Numerous new grants relied on our facility. There were at least three workshops that were sponsored by the Center that trained over 28 users on pioneering technical methodologies DEPTS OF College of Food, Agricultural and Natural Resource Sciences (1412h) Ag. Engineering (89); Animal Science (65); Agronomy & Plant Genetics (187); Entomology (340); Food Science & Nutrition (140); Plant Pathology (108) DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES (958h)Biotech. Inst. (173); Ecology Evo. Behav. (86); Genetics Cell Bio. Dev. (227); Imaging Center (160); Plant Biology (312) OTHER UM DEPARTMENTS (389h)Chemistry (121); Environ. Health (10); Fisheries Wildlife Cons Bio (63); Lab Med & Pathology (45); Mech Eng (9); Oral Sciences (4); Vet Pathobiology (43); Vet Biomed (61); Vet Clinical (28); Vet Population (5) NON-UNIVERSITY USERS (483h) - American Petrographics (1), ATG Laboratories (5), Aveda (35), Biothera (17), Cargill (20), Creative Associates (16), HealthPartners Rsrch Foundation (14), Immunochemistry Technologies,LLC (16), Intelligent Nutrients (12), Invitrogen (30), Life-Science Innovations (8), Land O'Lakes (18), Logic Product Development (23), Medtronic (38), NIOSH/CDC Spokane (28), Phillips Plastics (8), Receptor LLC (20), Schreiber Foods (52), Smiths Medical Md (42), Surmodics (18), WiXi AppTec (62) . CLASSES & TOURS (88) INSTRUMENT CALIBRATIONS & MAINTENENCE (30.0) PARTICIPANTS: To make this multiple-user facility productive requires an environment of user cooperation, and guidelines for usage. General policy is provided by, Mark Sanders, Program Director, through an Advisory Committee and is reinforced through constant contact with project leaders and users. The Imaging Center is staffed by Dr. Gail Ceilo, Mr. Gilbert Ahlstrand, Mr. Tracy Anderson and Student workers Mr. Alex Cramer, Ms. Chemi Banari, Ms. Kaitlyn Bissonnette and Ms. Lizzy Watkins. Significant subsidy toward cost of operation is provided by the College of Biological Sciences. MAES project leaders, their technical personnel, and research students have priority on equipment usage and on the assistance consultation provided by Mr. Ahlstrand. Other University of Minnesota research programs and industrial users have second priority to equipment usage. All fees subsidize operational costs. TARGET AUDIENCES: Our target audience includes participation from K-12 students and teachers; academic researchers at the university and college levels; corporate and industrial partners and governmental agencies. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

    Impacts
    The Imaging Center supports basic and multi-disciplinary applied research aimed at evaluating options for improving animal health and food safety through microscopic imaging technologies. This project revision continues MAES support funding, and ensures researchers access to state-of-the-art equipment and staff in these research efforts. Numerous grants rely on the facility and its staff. Several workshops open to the community on advanced imaging technologies have been held. Tours to K-12 students and teachers were well attended. Exhibits at the Minnesota Zoo and the Science Museum of Minnesota are based on results and collaborations.

    Publications

    • Castell-Miller, C. V., Szabo, L. J., Rosewich Gale, L., ONeill, N. R. and Samac, D. A. 2008. Molecular variability of Phoma medicaginis, the causal agent of spring black stem and leaf spot of alfalfa. Can. J. Plant Pathol. 30:85-96.
    • Chandran, D., Sharopova, N., VandenBosch, K. A., Garvin, D. F., and Samac, D. A. 2008. Physiological and molecular characterization of aluminum tolerance in Medicago truncatula. BMC Plant Biol. .8:89.
    • Shin S, Mackintosh CA, Lewis J, Heinen SJ, Radmer L, Dill-Macky R, Baldridge, GD, Zeyen RJ, Muehlbauer GJ. 2008. Transgenic wheat expressing a barley class II chitinase gene has enhanced resistance against Fusarium graminearum. Journal of Experimental Botany. 2008;59(9):2371-8.
    • Lewis JM, Mackintosh CA, Shin S, Gilding E, Kravchenko S, Baldridge G, Zeyen, RJ, Muehlbauer GJ. Overexpression of the maize Teosinte Branched1 gene in wheat suppresses tiller development. Plant Cell Reporter. 2008 Jul;27(7):1217-25.
    • Ratcliff, W.C., Kadam, S.V., and Denison, R.F. Poly-3-hydroxybutyrate (PHB) supports survival and reproduction in starving rhizobia. FEMS Microbiology Ecology, 65, (391-399), 2008
    • Smith AJ, Sanders MA, Juhlmann BE, Hertzel AV, Bernlohr DA. Mapping of the hormone-sensitive lipase binding site on the adipocyte fatty acid-binding protein (AFABP). Identification of the charge quartet on the AFABP/aP2 helix-turn-helix domain. J Biol Chem. 2008 Nov 28;283(48):33536-43. Epub 2008 Sep 27.
    • Cameron CM, Harding F, Hu WS, Kaufman DS. Activation of hypoxic response in human embryonic stem cell-derived embryoid bodies. Exp Biol Med (Maywood). 2008 Aug;233(8):1044-57. Epub 2008 Jun 5.
    • Huang Y, Li CY, Biddle KD, Gibson SI. Identification, cloning and characterization of sis7 and sis10 sugar-insensitive mutants of Arabidopsis. BMC Plant Biol. 2008 Oct 14;8:104.
    • Plesofsky NS, Levery SB, Castle SA, Brambl R. Stress-induced cell death is mediated by ceramide synthesis in Neurospora crassa. Eukaryot Cell. 2008 Dec;7(12):2147-59. Epub 2008 Oct 24.
    • Reinders A, Sivitz AB, Starker CG, Gantt JS, Ward JM. Functional analysis of LjSUT4, a vacuolar sucrose transporter from Lotus japonicus. Plant Mol Biol. 2008 Oct;68(3):289-99. Epub 2008 Jul 10.
    • Sivitz AB, Reinders A, Ward JM. Arabidopsis sucrose transporter AtSUC1 is important for pollen germination and sucrose-induced anthocyanin accumulation. Plant Physiol. 2008 May;147(1):92-100. Epub 2008 Mar 21.
    • Shin S, Mackintosh CA, Lewis J, Heinen SJ, Radmer L, Dill-Macky R, Baldridge GD, Zeyen RJ, Muehlbauer GJ. Transgenic wheat expressing a barley class II chitinase gene has enhanced resistance against Fusarium graminearum. J Exp Bot. 2008;59(9):2371-8. Epub 2008 May 7.
    • Lewis JM, Mackintosh CA, Shin S, Gilding E, Kravchenko S, Baldridge G, Zeyen R, Muehlbauer GJ. Overexpression of the maize Teosinte Branched1 gene in wheat suppresses tiller development. Plant Cell Rep. 2008 Jul;27(7):1217-25. Epub 2008 Apr 5.
    • Harmon, F., T. Imaizumi, and W.M. Gray. 2008. CUL1 regulates TOC1 protein stability in the Arabidopsis circadian clock. Plant J. 55: 568-579.
    • Piasecki BP, LaVoie M, Tam LW, Lefebvre PA, Silflow CD. The Uni2 phosphoprotein is a cell cycle regulated component of the basal body maturation pathway in Chlamydomonas reinhardtii. Mol Biol Cell. 2008 Jan;19(1):262-73. Epub 2007 Oct 17.
    • Stoebel DM, Dean AM, Dykhuizen DE. The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products. Genetics. 2008 Mar;178(3):1653-60. Epub 2008 Feb 3.
    • McLaughlin, D.J., G. J. Celio, M. Padamsee, and B.T.M. Dentinger. 2008. Cystidial structure in two genera of the Russulales. Botany 86: 545-550.
    • Jenkinson, T. S., G. J. Celio, M. Padamsee, and B.T.M. Dentinger, M. L. Meyer, and D. J. McLaughlin. 2008. Conservation of cytoplasmic organization in the cystidia of Suillus species. Mycologia 100: 539-547.
    • Padamsee, M, G. J. Celio, and D. J. McLaughlin. 2008. Reciprocal illumination: using phylogeny and ultrastructure to study cystidia of two Psathyrella species. Botany 86: 1-9.
    • Schottel JL, Orwin PM, Anderson CR, Flickinger MC. Spatial expression of a mercury-inducible green fluorescent protein within a nanoporous latex-based biosensor coating. J Ind Microbiol Biotechnol. 2008 Apr;35(4):283-90. Epub 2008 Jan 8.
    • Piasecki, B.P., LaVoie, M., Ta., L.W., Lefebvre, P.A., and Silflow, C.D. (2008) The Uni2 phosphoprotein is a cell cycle-regulated component of the basal body maturation pathway in /Chlamydomonas reinhardtii/. Mol. Biol. Cell 19:262-273.
    • Piasecki B.P. and Silflow, C.D. (2009) The /UNI1/ and /UNI2 /genes function in the transition of triplet to doublet microtubules between the cenetriole and cilium in /Chlamydomonas/. Mol. Biol. Cell 20:368-378.
    • Sander JD, Zaback P, Joung JK, Voytas DF, Dobbs D. An affinity-based scoring scheme for predicting DNA-binding activities of modularly assembled zinc-finger proteins. Nucleic Acids Res. 2008 Dec 4. [Epub ahead of print].
    • Blanchette, R. A., B. W. Held and J. A. Jurgens. 2008. Northumberland House, Fort Conger and the Peary Huts in the Canadian High Arctic: current condition and assessment of wood deterioration taking place. In: Historical Polar Bases Preservation and Management. Edited by S. Barr and P. Chaplin. ICOMOS Monuments and Sites No.XVII. International Polar Heritage Committee, Oslo, Norway. pp.96.
    • Perez, C. A. 2008. Relationship between pathogens of Eucalyptus and native Myrtaceae in Uruguay. PhD Dissertation University of Minnesota 125 pp.
    • Arenz, B. A. and R. A. Blanchette. 2008. East Base, SOS: Assessment of deterioration and recommendations for conserving this important Antarctic site. In: Historical Polar Bases Preservation and Management. Edited by S. Barr and P. Chaplin. ICOMOS Monuments and Sites No.XVII. International Polar Heritage Committee, Oslo, Norway pp.96.
    • Richards, V., R. A. Blanchette, I. Godfrey, D. Gregory, B. Held and E. Reed. 2008. In Situ Monitoring and Stabilisation of the James Matthews Site, In Proceedings of the 10th ICOM Group on Wet Organic Archaeological Materials Conference, Amsterdam, 10-15 September 2007, The International Council of Museums, Committee for Conservation Working Group on Wet Organic Archaeological Materials.
    • Jacobs, J. J. 2008. Histopathology of white pine blister rust in eastern white pine (Pinus strobus L.). MS Thesis University of Minnesota 36pp.
    • Seong, K., Zhao, X., Xu, J.-R., Guldener, U., and Kistler, H.C. Conidial germination in the filamentous fungus Fusarium graminearum. Fungal Genetics and Biology. 45. 389-399. 2008.
    • Chandran, D., Sharopova, N., Ivashuta, S., Gantt, J. S., VandenBosch, K. A., and Samac, D. A. 2008. Transcriptome profiling identified novel genes associated with aluminum toxicity, resistance and tolerance in Medicago truncatula. Planta 228:151-166.
    • Zhang L, Lockhart B, Dahal G, Olszewski N. Studies on biology and genomic characterization of a caulimo-like virus associated with a leaf distortion disease of Lamium maculatum. Arch Virol. 2008;153(6):1181-4. Epub 2008 May 6.
    • Jitacksorn, S., and M. J. Sadowsky. 2008. Nodulation gene regulation and quorum sensing control density-dependent suppression and restriction of nodulation in the Bradyrhizobium japonicum-soybean symbiosis. Appl Environ Microbiol. 74:3749-3756.
    • Walz A, Seidel C, Rusak G, Park S, Cohen JD, Ludwig-Muller J. Heterologous expression of IAP1, a seed protein from bean modified by indole-3-acetic acid, in Arabidopsis thaliana and Medicago truncatula. Planta. 2008 Apr;227(5):1047-61. Epub 2007 Dec 21.
    • Marshall, W. L., G. J. Celio, D. J. McLaughlin, and M. L. Berbee, 2008. Multiple isolations of a culturable, motile Mesomycetozoan, Creolimax fragrantissima n. gen., n. sp., from marine invertebrate digestive tracts. Protist 159: 415-433.
    • Marsili E, Rollefson JB, Baron DB, Hozalski RM, Bond DR. Microbial biofilm voltammetry: direct electrochemical characterization of catalytic electrode-attached biofilms. Appl Environ Microbiol. 2008 Dec;74(23):7329-37. Epub 2008 Oct 10.
    • Reinders A, Sivitz AB, Starker CG, Gantt JS, Ward JM. Functional analysis of LjSUT4, a vacuolar sucrose transporter from Lotus japonicus. Plant Mol Biol. 2008 Oct;68(3):289-99. Epub 2008 Jul 10.
    • Fallon, 2008: Cytological properties of an Aedes albopictus mosquito cell line infected with Wolbachia strain wAlbB, In Vitro Cell Develop BiolAnimal 44, 154-161. NIHMSID 63476.
    • Fallon, AM and Hellestad, VJ, 2008: Standardization of a colorimetric method to quantify cell growth and metabolic activity in Wolbachia-infected mosquito cells, In vitro cell develop BiolAnimal 44: 351-356. NIHMSID 70077.
    • Zhang Z, Pendse ND, Phillips KN, Cotner JB, Khodursky A. Gene expression patterns of sulfur starvation in Synechocystis sp. PCC 6803. BMC Genomics. 2008 Jul 21;9:344.
    • Uc-Mass A, Khodursky A, Brown L, Gottesman ME. Overexpression of phage HK022 Nun protein is toxic for Escherichia coli. J Mol Biol. 2008 Jul 25;380(5):812-9. Epub 2008 May 17.
    • Marks MD, Betancur L, Gilding E, Chen F, Bauer S, Wenger JP, Dixon RA, HaiglerCH. A new method for isolating large quantities of Arabidopsis trichomes for transcriptome, cell wall and other types of analyses. Plant J. 2008 Nov;56(3):483-92. Epub 2008 Aug 4.
    • Pan JJ, Baumgarten AM, May G. Effects of host plant environment and Ustilago maydis infection on the fungal endophyte community of maize (Zea mays). New Phytol. 2008;178(1):147-56. Epub 2008 Jan 10.


    Progress 01/01/07 to 12/31/07

    Outputs
    OUTPUTS: Since the period of the last renewal of this project, 206 different projects and 194 instrument users representing over a thousand individual equipments uses have done research on the instrumentation component in collaboration with the Imaging Center. We are a state-of-the-art core facility for imaging of biological specimens utilizing light microscopy; scanning and transmission electron microscopy and digital imaging; related to research, training and education. Briefly, the instrumentation and resources of the Imaging Center have been used in the discovery of new viruses and other microbes of plants and animals; the examination of cells and organelles in plant and animal tissue culture; biomedical device and reagent integration; experimental composition of food mixtures and dairy projects, elemental composition and morphology of soil particles and their importance in pollution-related research; the elemental composition of plants as related to practical problems in crop plant physiology and health; deterioration of textiles and forest products; various bioprocessing projects designed to lessen the environmental impact of paper and forest product production; renewable energy projects; nitrogen fixation of plants; alternatives to conventional insecticides; the adherence of pathogenic bacteria to food and food processing surfaces; analysis of food storage materials; the identification and mapping of fungal chromosomes; and for the in situ identification of the activity of various genes of importance during genetic engineering of plants; as well as pioneering technical methodologies. USERS In the past reporting period, the usage of the major instrumentation totaled 2597 hours: Electron microscopes (TEM, SEM), x-ray microanalysis equipment, cryo-stage; includes users, calibration, service, class demonstrations & tours - 893 hours. Light microscopes (confocal, inverted, upright); includes users, classes, demonstrations & tours -1704 hours. There have been over 2000 research and instructional posters printed. There were 6 Masters or PhD degrees that acknowledged the facility. Numerous new grants relied on our facility. DEPTS OF College of Food, Agricultural and Natural Resource Sciences (630) Ag. Engineering (17); Animal Science (34); Agronomy & Plant Genetics (143); Entomology (310); Food Science & Nutrition (121); Plant Pathology (65) DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES (623h) Biotech. Inst. (182.5); Ecology Evo. Behav. (91); Genetics Cell Bio. Dev. (111); Imaging Center (140); Plant Biology (269) BMBB (218) OTHER UM DEPARTMENTS (279h) Chemistry (106); Environ. Health (1); Fisheries Wildlife Cons Bio (43); Lab Med & Pathology (12); Mech Eng (15); Oral Sciences (4); Restorative Sciences (1); Vet Pathobiology (18); Vet Biomed (43); Vet Clinical (38); Vet Population (3) NON-UNIVERSITY USERS (322h) - American Petrographics (1), ATG Laboratories (5), Aveda (17), Biothera (47), Boston Scientific (20), Cargill (120), Gel-Del technologies (67), Isurtec (8), Land O'Lakes (32), Medtronic (26), NIOSH/CDC Spokane (32), Phillips Plastics (8), R&D Systems (3), Schreiber Foods (40), Smiths Medical Md (30). CLASSES & TOURS (80). Maintenance (25) PARTICIPANTS: 194 instrument users representing over a thousand individual equipment uses have utilized the instrumentation component in collaboration with the Imaging Center. There were 6 Masters or PhD degrees that acknowledged the facility.A breakdownof the usage by unit is as follows: DEPTS OF College of Food, Agricultural and Natural Resource Sciences (630) Ag. Engineering (17); Animal Science (34); Agronomy & Plant Genetics (143); Entomology (310); Food Science & Nutrition (121); Plant Pathology (65) DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES (623h) Biotech. Inst. (182.5); Ecology Evo. Behav. (91); Genetics Cell Bio. Dev. (111); Imaging Center (140); Plant Biology (269) OTHER UM DEPARTMENTS (279h) Chemistry (106); Environ. Health (1); Fisheries Wildlife Cons Bio (43); Lab Med & Pathology (12); Mech Eng (15); Oral Sciences (4); Restorative Sciences (1); Vet Pathobiology (18); Vet Biomed (43); Vet Clinical (38); Vet Population (3) NON-UNIVERSITY USERS (322h) - American Petrographics (1), ATG Laboratories (5), Aveda (17), Biothera (47), Boston Scientific (20), Cargill (120), Gel-Del technologies (67), Isurtec (8), Land O'Lakes (32), Medtronic (26), NIOSH/CDC Spokane (32), Phillips Plastics (8), R&D Systems (3), Schreiber Foods (40), Smiths Medical Md (30). CLASSES & TOURS (80). The oversight of the Imaging Center is the primary responsibility of M.A. Sanders. TARGET AUDIENCES: The Center offers training, special workshops and courses for faculty and staff members, students, postdoctoral scholars, Cooperative Extension personnel, corporate scientists, and others. These courses focus on topics in imaging, such as sample processing and preparation, confocal microscopy, electron microscopy and bioinformatics. The Imaging Center also has assisted in development of curricula for high school and community college teachers.

    Impacts
    The Imaging Center supports basic and multi-disciplinary applied research aimed at evaluating options for improving animal health and food safety through microscopic imaging technologies. This project revision continues MAES support funding, and ensures researchers access to state-of-the-art equipment and staff in these research efforts. Numerous grants rely on the facility and its staff. Workshops open to the community on advanced imaging technologies have been held. Exhibits at the Minnesota Zoo and the Science Museum of Minnesota are based on results and collaborations.

    Publications

    • Peng Zhou, Theodore P. Labuza. 2007. Effect of Water Content on Glass Transition and Protein Aggregation of Whey Protein Powders During Short-Term Storage. Food Biophysics. DOI 10.1007/s11483-007-9037-4.
    • Ameline-Torregrosa, C., Cazaux, M., Danesh, D., Chardon, F., Cannon, S. B., Esquerre-Tugaye,M.-T., Dumas, B., Young, N. D., Samac, D. A., Huguet, T., and Jacquet, C., 2008. Genetic dissection of resistance to anthracnose and powdery mildew in Medicago truncatula. Mol. Plant-Microbe Interact. 21:61-69.Archives of virology, 152 : 1409-141
    • Castill-Miller, C.V., Zeyen, R.J., Samac, D.A. 2007. Infection and development of Phoma medicaginis on moderately resistant and susceptible alfalfa genotypes. Canadian Journal of Plant Pathology 29: 290-298.
    • D.J. Weiss, Cleverson Souza , Oral Evanson , Mark Sanders , Mark Rutherford.Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. Avium. Journal of Leukocyte Biology. 2007.
    • Dimitre S. Mollov, Maya C. Hayslett, Kari A. Eichstaedt, Noelle G. Beckman, Margery L. Daughtrey, and Ben E. Lockhart. Identification and Characterization of a Carlavirus Causing Veinal Necrosis of Coleus Plant Disease Jun 2007, Volume 91, Number 6: 754-757.
    • Foster-Hartnett, D., Danesh, D., Penuela, S., Sharopova, N., Endre, G., VandenBosch, K. A., Young, N.D., and Samac, D. A. 2007. Molecular and cytological responses of Medicago truncatula to Erysiphe pisi. Mol. Plant Pathol. 8:307-319.
    • Lheureux F., Laboureau N., Muller E., Lockhart B.E.L., Iskra Caruana M.L.. 2007. Molecular characterization of banana streak acuminata Vietnam virus isolated from Musa acuminata siamea (banana cultivar).
    • Kelsey Dahl, Jim Perry, Mark Sanders. 2007. Effects of domoic acid on the gonadotropin releasing hormone (GT1-7) neuronal cell line. BIOS 78(4)000-000.
    • Mackintosh, C. A., J. Lewis, L. E. Radmer, S. Shin, S. J. Heinen, L. A. Smith, M. N. Wyckoff, R. Dill-Macky, C. K. Evans, S. Kravchenko, G. D. Baldridge, R. J. Zeyen, and G. J. Muehlbauer. 2007. Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight. Plant Cell Reports 26:479-488.
    • Miller A, Ahlstrand G, Kittelson D, Zachariah M. 2007. The fate of metal (Fe) during diesel combustion: Morphology, chemistry, and formation pathways of nanoparticles. Combustion and Flame 149 129-143.
    • Miller, A.L., Stipe, C., Habjan, M.C., Ahlstrand, G., 2007. Role of Lubrication Oil in Particulate Emissions from a Hydrogen-Powered Internal Combustion Engine. Environ. Sci. Technol., 41, 6828-6835.
    • Morey, R.V., N. Kaliyan, M. White, and A. Doering. 2007. Roll press briquetting of corn stover and switchgrass. Proceedings of the Institute for Briquetting and Agglomeration 30th Biennial Conference. Manitowish Waters, WI: IBA.
    • Pasburg-Gauhl, C., Tafrilico, S.A., Lockhart, B.E.L., Castro-Mendivil Diobos, F., Rojas Llanque, J.C. 2007. Banana streak virus Identified for the First Time in Peru in Cavendish Banana (Musa AAA). Plant Dis. 91:906
    • Sanders, M.A, Anderson, T.E., Giberson, R. (2006) Microwave methods - evidence to support a microwave effect. Microsc. Microanal. 12(Suppl.2:Proceedings CD):295-296.
    • Schnurr, J. A., Jung, H. G., and Samac, D. A. 2007. A comparative study of alfalfa and Medicago truncatula stem traits: Morphology, chemical composition, and ruminal digestibility. Crop Sci. 47:1672-1680.
    • Sivitz, Reinders, Ward. 2007. Arabidopsis Sucrose Transporter AtSUC9. High-Affinity. Plant Physiology. 143:188-198
    • Smith AJ, Thompson BR, Sanders MA, Bernlohr DA. Interaction of the adipocyte fatty acid-binding protein with the hormone-sensitive lipase: regulation by fatty acids and phosphorylation. J Biol Chem. 2007 Nov 2;282(44):32424-32.


    Progress 01/01/06 to 12/31/06

    Outputs
    Since the period of the last renewal of this project, 386 different projects and 251 instrument users representing over twenty-three hundred individual equipments uses have done research on the instrumentation component of Imaging Center. Their known published results, as voluntarily reported, accompany this renewal. Space does not permit an elaboration of the technical procedures used by these many and varied research efforts. Briefly, the instrumentation and resources of the Imaging Center have been used in the discovery new viruses and other microbes of plants, animals and bacteria; the examination of cells and cell organelles during plant and animal tissue basic research; biomedical device and reagent integration; experimental composition of food mixtures in feed and dairy projects, elemental composition and morphology of soil particles and their importance in pollution-related research; the elemental composition of plants as related to practical problems in crop plant physiology and health; deterioration of textiles and forest products; various bioprocessing projects designed to lessen the environmental impact of paper and forest product production; renewable energy projects; nitrogen fixation of plants; alternatives to conventional insecticides; the adherence of pathogenic bacteria to food and food processing surfaces; analysis of food storage materials; the identification and mapping of fungal chromosomes; and for the in situ identification of the activity of various genes of importance during genetic engineering of plants. To make a multiple-user facility productive requires an environment of user cooperation, and guidelines for usage. General policy is provided by the Program Director through an Advisory Committee and is reinforced through constant contact with project leaders and users. MAES project leaders, their technical personnel, and research students have priority on equipment usage and on the assistance. Other University of Minnesota research programs and industrial users have second priority to equipment usage. Since they do not represent MAES research programs, a higher user fee structure has been charged. All fees subsidize operational costs. Total hours used for major instrumentation (2545) hours: Electron microscopes (TEM, SEM), x-ray microanalysis equipment, cryo-stage; includes calibration, service, class demonstrations & tours. - (931) hours. Light microscopes (confocal, inverted, upright, stereo) - (1614) hours. USERS DEPTS OF COLLEGE OF AG, FOOD & ENV SCI, MAES/ CFANS ( 468.5h) DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES (542.0h) OTHER UM DEPARTMENTS (206.75h) OUTSIDE UNIVERSITIES & COLLEGES (46.75h) NON-UNIVERSITY USERS (408.5) - American Petrographics; ATG Laboratories; Aveda; Biothera; Boston Scientific; Cargill; Center for Disease Control; GeneSegues, Inc.; Gel-Del technologies; Inovative Surface Technologies; Isurtec; Land O'Lakes; Medtronic; Nanocopoeia; NIOSH/CDC Spokane; Phillips Plastics; Surmodics; R&D Systems; Schreiber Foods; Smiths Medical Md. CLASSES & TOURS (146.5h) INSTRUMENT CALIBRATIONS & MAINTENENCE (107h)

    Impacts
    The Imaging Center facilitates basic and multi-disciplinary applied research aimed at evaluating options for improving animal health and food safety through microscopic technologies. This project revision continues MAES support funding, and ensures researchers in the region access to state-of-the-art equipment and staff for these research efforts.

    Publications

    • Perdigao J, Gomes G, Ahlstrand G G, Augusto V, Franco P, Pinto A. 2006. SEM/TEM interfacial morphology of adhesive materials to root canal dentin. Proceedings of the American Association for Dental Research and The American Dental Education Association. March 8-11, Orlando, FL.
    • Wymelenberg, A.V., G. Sabat, M. Mozuch, P. J. Kersten, D. Cullen and R. A. Blanchette. 2006. Structure, Organization, and Transcriptional Regulation of a Family of Copper Radical Oxidase Genes in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium. Applied Environmental Microbiology 72:4871-4877. *
    • Duncan, S., R. L. Farrell, J. M. Thwaites, B. W. Held, B. E. Arenz, J. A. Jurgens and R. A. Blanchette. 2006. Endoglucanase-producing fungi isolated from Cape Evans historic expedition hut on Ross Island, Antarctica. Environmental Microbiology 8:1212-1219.
    • Smith, J.A., Blanchette, R.A., Burnes, T.A., Gillman, J.H. and A. J. David. 2006. Epicuticular wax and white pine blister rust resistance in selections of Pinus strobus L. Phytopathology 96: 171-177.
    • Held, B. W., J.A. Jurgens, S.M. Duncan, R.L. Farrell and R.A. Blanchette. 2006. Assessment of fungal diversity and deterioration in a wooden structure at New Harbor, Antarctica. Polar Biology 29:526-531. *
    • Partial list of Publications Project 22-62 2006
    • Ahlstrand GG, Miller A L, Stipe C. B. 2006. Characterization of Particulate Emissions from a Hydrogen Engine. Microscopy & Microanalysis 12(Supp 2), p.794-795CD.
    • Miller A L, Stipe C B, Habjan M C, Ahlstrand GG. 2006. The Role of Lubrication Oil in Particulate Matter Emissions from a Hydrogen Engine. Proceedings of the 7th International Aerosol Conference, St. Paul, MN.
    • Tamada H, Thuan N V, Reed P, Nelson D, Katoku-Kikyo N, Wudel J, Wakayama T, Kikyo N. 2006. Chromatin decondensation and nuclear reprogramming by nucleoplasmin. Mol. Cell. Biol. 2006 26: 1259-1271.
    • PhD Thesis: Physiological and genomic aproaches to study aluminum toxicity and tolerance in the model legume Medicago truncatula. 2006. Student: Divya Chandran Advisor: Debrah Samac.
    • Tweeten, K. A., Curtis, J., Derby, R., Seefeld, M., and Vang, L. 2006. Molecular Analysis of the Extracellular Hemoglobin from Lumbriculus variegates.
    • Boddu, J., S. Cho, W.M. Kruger and G.J. Muehlbauer. 2006. Transcriptome analysis of the barley-Fusarium graminearum interaction. Mol. Plant-Microbe Interact. 19:407-417.
    • Tamada, H., Thuan, N. V., Reed, P., Nelson, D., Katoku-Kikyo, N., Wudel, J., Wakayama, T.and Kikyo, N. (2006) Chromatin decondensation and nuclear reprogramming by nucleoplasmin. Mol Cell Biol 26, 1259-71.
    • Gonda, K., Wudel, J., Nelson, D., Katoku-Kikyo, N., Reed, P, Tamada, H. and Kikyo, N. (2006) Requirement of the protein B23 for nucleolar disassembly induced by the FRGY2a family proteins. J Biol Chem 281, 8153-60.
    • Hertzel, A.V., Smith, L.S., Berg, A.Hl, Cline, G.V., Shulman, G.I., Scherer, P., and Bernlohr, D.A. 2006. Lipid Metabolism and Adipokine Levels in Fatty Acid Binding Protein Null and Transgenic Mice. Am. J. Physiol. Endo. Metab, 290, E814-E823.
    • Lobo, S., Wiczer, B., and Bernlohr, D.A. Fatty Acid Transport in Adipocytes. Future Lipidology. 1, 291-298. 2006.
    • Jose J. Galvez, Grete Adamson, Mark A. Sanders, Richard T. Giberson. Microwave Tissue Processing Techniques: Their Evolution and Understanding. Microscopy and Microanalysis. Volume 20(6) November 2006.


    Progress 01/01/05 to 12/31/05

    Outputs
    Since the period of the last renewal of this project, 206 different projects and 194 instrument users representing over a thousand individual instrumentation uses have done research using the instrumentation component of Imaging Center. Their published results, as voluntarily reported, accompany this renewal. Space does not permit an elaboration of the technical procedures used by these many and varied research efforts. Briefly, the instrumentation and resources of the Imaging Center have been used in the discovery new viruses and other microbes of plants and animals; the examination of cells and organelles in plant and animal tissue culture; biomedical device and reagent integration; experimental composition of food mixtures and dairy projects, elemental composition and morphology of soil particles and their importance in pollution-related research; the elemental composition of plants as related to practical problems in crop plant physiology and health; deterioration of textiles and forest products; various bioprocessing projects designed to lessen the environmental impact of paper and forest product production; renewable energy projects; nitrogen fixation of plants; alternatives to conventional insecticides; the adherence of pathogenic bacteria to food and food processing surfaces; analysis of food storage materials; the identification and mapping of fungal chromosomes; and for the in situ identification of the activity of various genes of importance during genetic engineering of plants. General policy is provided by the Program Director through an Advisory Committee and is reinforced through constant contact with project leaders and users. USERS-total hours used for major instrumentation: Electron microscopes (TEM, SEM), x-ray microanalysis equipment, cryo-stage; includes users, calibration, service, class demonstrations & tours - 858.5 hours. Light microscopes (confocal, inverted, upright); includes users, calibration, service, class demonstrations & tours -1302 hours. Total major instrumentation usage 2160.5 hours. DEPTS OF COLLEGE OF AG, FOOD & ENV SCI, MAES (560h) Ag. Engineering (17); Animal Science (18); Agronomy & Plant Genetics (122); Entomology (265); Food Science & Nutrition (34); Plant Pathology (104) DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES (607h) Biotech. Inst. (122.5); Ecology Evo. Behav. (81.25); Genetics Cell Bio. Dev. (61.25); Imaging Center (132.5); Plant Biology (209.5) OTHER UM DEPARTMENTS (187h) Chemistry (86); Environ. Health (1); Fisheries Wildlife Cons Bio (43); Lab Med & Pathology (1); Mech Eng (15); Oral Sciences (1); Restorative Sciences (1); Vet Pathobiology (8); Vet Biomed (13); Vet Clinical (18); Vet Population (1) NON-UNIVERSITY USERS (718h) - American Petrographics (1). ATG Laboratories (2). Aveda (7). Biothera (47.0). Boston Scientific (320), Gel-Del technologies (87), Isurtec (8). Land OLakes (32). Medtronic (26). Nanocopoeia (116). NIOSH/CDC Spokane (22). Phillips Plastics (3). R&D Systems (2). Schreiber Foods (19). Smiths Medical Md (26). CLASSES & TOURS (62.5) INSTRUMENT CALIBRATIONS & MAINTENENCE (25.0)

    Impacts
    The Imaging Center facilitates basic and multi-disciplinary applied research aimed at evaluating options for improving animal health and food safety through microscopic technologies. This project revision continues MAES support funding, and ensures researchers access to state-of-the-art equipment and staff for these research efforts.

    Publications

    • Ihmels J, Bergmann S, Berman J. and Barkai N. 2005. The Differential Clustering for comparative gene expression analysis: application to the Candida albicans transcription program. PloS Genetics. 1: e39;0380-0393.
    • Henson, MS. 2005. Comparative Models of Type 2 Diabetes Mellitus: The Role of Islet Amyloid Polypeptide in Pathogenesis. PhD thesis, advs TD O'Brien & RM Hardy. University of Minnesota, St. Paul, MN
    • Alghamdi, AS and Foster DN. 2005. Seminal DNase frees spermatozoa entangled in neutrophil extracellular traps. Biol. Reproduction73:1174-1181.
    • Alghamdi, AS. Characterization of Breeding Induced Endometritis in the Mare: The Role of Seminal Plasma and Nirtic Oxide. 2005. Ph.D thesis. Advs M Troedsson and DN Foster, University of Minnesota, St. Paul, MN
    • Partridge RF. 2005. The Effects of CO2 and O3 on the interaction between Phyllonorycter apparella Herrich-Schaffer (Gracillariidae) and Populus tremuloides Michx. MS thesis. Adv KE Percy. Michigan Tech University.
    • Zeyen RJ, Kruger WM. 2005. Fast, intermediate and slow acting barley powdery mildew R genes influence transcription patterns of basal defense response genes. Phytopathology S pp. 122.
    • Lewis JM, Mackintosh CA, Shin SH, Smith LA, Wyckoff MN, Elakkad A, Wennberg K, Heinen SJ, Radmer LE, Baldridge GD, Zeyen RJ, Evans CK, Kravchenko S, Dill-Macky R, Muehlbauer GJ. 2005. Overexpression of Antifungal Proteins Increases Resistance of Wheat to Fusarium Head Blight in the Field. National Fusarium Head Blight Forum. Milwaukee, Wisconsin.
    • Lockhart BEL. 2005. Three previously unrecorded viral diseases of Astilbe, Fuschia, and Thermopsis species in Minnesota. Plant Dis. 89:775 N.
    • Yang Z, Ncolaisen M, Olszewski, N, and Lockhart BEL. 2005. Sequencing , improved detection and a novel form of Kalanoche top spotting virus. Plant Dis. 89: 298-302.
    • Piasecki BP, Wu W, LaVoie M, Tam LW, Lefebvre PA, Silflow CD. 2005. The UNI2 gene in Chlamydomonas reinhardtii encodes a component of basal bodies and probasal bodies. Mol. Biol. of the Cell, Vol 16. Poster, American Society for Cell Biology 45th Annual Meeting, San Francisco, CA
    • Nguyen RL, Tam LW, and Lefebvre PA. 2005. The LF1 gene of Chlamydomonas reinhardtii encodes a novel protein required for flagellar length control. GeneticsMar;169(3):1415-24.
    • Kuppusamy KT. 2005. Genetic and Genomic Approaches to Dissect Nodulation in the Model Legume Medicago truncatula. PhD thesis, adv KA VandenBosch. University of Minnesota, St. Paul, MN
    • Perdigao J, Lopes MM, Ahlstrand GG. Characterization of Non-Decalcified Adhesive Interfaces with TEM and EDS. Journal of Dental Research 2005; 84(Spec Iss A): abstract number 0161 IADR/AADR/CADR 83rd General Session in Baltimore, MD
    • Blaedow R, Ahlstrand G, Barber B. Juzwik J. 2005. Detection and quantification of propiconazole in oak tissues using microwave extraction and gas chromatography-mass spectrometry. Phytopathology 95:S10.
    • Flor LB, Kurtti TJ, Munderloh U. 2005. Characterization of a Beauveria bassianaisolate from feral black-legged ticks, Ixodes scapularis(Say). 2005. Abstract. 38th Annual Meeting of the Society for Invertebrate Pathology. Anchorage Alaska.
    • Kurtti TJ, Simser JA, Baldridg, GD, Palmer AT, Munderloh UG. 2005. Factors influencing in vitro infectivity and growth of Rickettsia peacockii (Rickettsiaceae), an endosymbiont of the Rocky Mountain wood tick, Dermacentor andersoni(Acari, Ixodidae). J. Invertebr. Pathol. 90:177-186.
    • Munderloh UG, Jauron SD, Kurtti TJ. 2005. The tick: a different kind of host for human pathogens. In: Goodman JL, Denni, D, Sonenshine DE(Eds.) Tick-Borne Diseases of Humans. ASM Press, Washington DC, pp. 37-64.
    • McNeil KJ, Smith AG. 2005. An anther-specific cysteine-rich protein of tomato localized to the tapetum and microspores. J. Plant Physiol. 162: 457-464.
    • Westberg, CR 2005. Characterization of Glycine-Rich Proteins in Arabidopsis thaliana and Lycopersicon esculentu. MS Thesis, AG Smith, adv. University of Minnesota, St. Paul, MN.
    • Mann HD, Bedford D, Luby J, Vickers Z, Tong C. 2005. Relationship of instrumental and sensory texture measurements of fresh and stored apples to cell number and size. HortScience 40:1815-1820.
    • Crampin H, Finley K, Gerami-Nejad M, Court H, Gale C, Berman J, Sudbery P. 2005. Candida albicans hyphae have a Spitzenkorper that is a distinct structure from the polarisome found yeast and pseudohyphae. J. Cell Sci. 118:2935-47.
    • Bensen ES, Clemente-Blanco A, Finley KR, Correa-Bordes J, Berman, J. 2005. The Mitotic Cyclins Clb2p and Clb4p Affect Morphogenesis in Candida albicans. Mol Biol Cell. 16:387-400.
    • Finley F, Berman J. 2005. Microtubules in C. albicans hyhpae drive nuclear dynamics and connect cell cycle progression to morphogenesis. Eukaryotic Cell, 4 (10): Cover Article.
    • Garrido MJ, Ordosgoitti A, Lockhart BEL. 2004. Presence of Banana streak virus-OL in dessert bananas in Maracay, Venezuela. J. Plant Pathol. 86: 263-264.
    • Garrido MJ, Ordosgoitti A, Lockhart BEL 2005. Identification del virus del rayado del banano en Venezuela. Interciencia 30: 97-101.
    • Blanchette B, Lockhart BEL. 2005. Hosta virus x: a three-year study. Hosta J. 35:19-23.


    Progress 01/01/03 to 12/31/03

    Outputs
    Total hours used:Electron microscopes, x-ray microanalysis equipment, cryo-stage; includes calibration, service, class demonstrations & tours-(791.25) hours.PROJECT LEADERS & USERS. DEPTS OF COLLEGE OF AG, FOOD & ENV SCI, MAES(143.25h) ENTOMOLOGY(34.75)-Project Leaders: Kurtti,Munderloh. User:Flor. FOOD SCIENCE & NUTRITION(5.5)-Project Leader:Reineccius.Users: Luo. HORTICULTURAL SCIENCE(6.0)-Project Leaders:A.Smith,Erwin. Users:A.Smith,Westberg, Warner. PLANT PATHOLOGY(97.0)-Project Leaders:Blanchette,Lockhart,Samac,Bushnell,Young, Juzwik.Users:Ahlstrand,Lockhart,Castell,Bushnell,Krueger,Jurgens,Held ,Seeland, J.Smith,Danesh, Eggers. DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES(134.5h) IMAGING CENTER(8.5) Project Leader:Sanders. Users:Sanders,Ahlstrand. B.T.I.(23.0)-Project Leaders:Wackett,Srienc; Users: Dodge,Strong,Pederson. GENETICS CELL BIO. DEV.(15.25)-Project Leaders:Neufeld,Kuriyama, Wright. Users:Kuriyama,Ahlstrand,Scott,Jauert. PLANT BIOLOGY(87.75)-Project Leaders: Marks, McLaughlin; Users: Marks, Esch, McLaughlin, Celio, Padamsee, Dentinger. OTHER UM DEPTS (159.75h) CLINICAL & POP. SCIENCES(17.5) Project Leader:Pijoan. Users:Cardona,Eduarto. FISHERIES WILDLIFE CONS BIO(12.5).Project Leaders:Hove,Strong. Users:Hove,Bloemke,Tara. LAB MED & PATHOLOGY(4.75)-Project Leader: J.White. Users:J.White,Ahlstrand. MECH ENG(43.25) Project Leader:Zachariah. Users:Miller,Ahlstrand,Raj,B.Zhang,Parthangal. MEDICINE(10.5) Project Leader:Kikyo. Users:Kikyo,Josephsen. PEDIATRICS(13.0)-Project Leader:Gale. Users: Berman,Ahlstrand. SURGERY(36.5)-Project Leader:Beilman. Users:G.Josephsen,K.Josephsen. VET DIAG LAB(5.25) Project Leader:Borjesson. Users:Borjesson,Aber,Argue,Ahlstrand. SMALL ANIMAL CLIN. SCI.(2.25) Project Leader:Osborne. Users:Ulrich,Ahlstrand. DERMATOLOGY(6.0) Project Leader:Hordinski. Users:Lilly,Lander,Josephsen. RESTORATIVE SCIENCES(4.0) Project Leader: Perdigao. Users:Lopes,Perdigao. NRRI Ctr. Water, Envon.(4.25) Project Leader:Kingston. Users: Kingston,Kireta,Ahlstrand. OUTSIDE UNIVERSITIES & COLLEGES(25.75) Clemson University(8.25 Wourms,Krueger), U.Wisconsin,River Falls(13.5 Mogen), St.OlafCollege(4.0 Kandl,Ahlstrand). NON-UNIVERSITY USERS(166.5)-Medtronic (14.75 Hefelfinger,Tenbroek,Coscio,Harris),Phillips Plastics (7.25 Fitch,Entezarian), American Petrographics(18.0 Edwards,Wolter),SIMS Deltec (21.5 Johnson), Aveda(9.0 Freundschuh,Hayes,Sarah,Jeff,Jessica),American Eng.Testing(2.25, Ferdowski), NIOSH/CDC Spokane(13.75 Miller), Surmodics 10.0 Anderson,Tran),Spineology(14.25 Wolfe), General Mills(2.5 Casper),Tru-Fresh Marketing(30.25 Lieberman).HearthTech.(3.0 Mix),LandO'Lakes (8.75 Bloomer),SchreiberFoods(7.0 Brandsma),MerlinDevelopment(4.25 Thompson).CLASSES & TOURS (30.25)-Project Leaders- nternational High School,CBS Student Services,CBS Itasca Teachers,Industrial Microbiologists,CBS Community Day,Macalester College(Parsons),MacDonald,Percich,Kurle,Lockhart,Bantarri,Olszewski, Vance,Elde,Sanders, von Keitz.INSTRUMENT CALIBRATIONS & MAINTENENCE(131.25)-Imaging Center(82.5 Ahlstrand).FEI. SERVICE(16.0 Olsen).HITACHI INSTRUMENTS SERVICE(26.0 Griffin).EDAX 6.75 Swada)

    Impacts
    The project supports the electron optical imaging and X-ray microanalysis needs of researchers employed by the Minnesota Agricultural Experiment Station. Instrumentation in the facility was purchased by grants from the National Science Foundation, from MAES funds and from funds available from the Graduate School of the University of Minnesota and the College of Biological Sciences. To augment Electron Optical Facility finances for operation, excess instrument time is made available for a fee to other University of Minnesota research programs and to local agribusiness, biomedical and engineering businesses.

    Publications

    • Lheureux F, Carreel F, Jenny C, Lockhart BEL & Iskra-Caruana ML. 2003. Identification of genetic markers linked to banana streak disease expression in inter-specific Musa hybrids. Theor. Appl. Genet. 106:594-598.
    • Ewing HA & Nater EA. 2003. Use of scanning electron microscopy to investigate records of soil weathering preserved in lake sediment. Holocene 13(1):51-60.
    • Ahlstrand, GG. Cellulose acetate replication of plant surfaces for SEM. 2003. Microscopy Today. Vol. 11 #6, p53.
    • White JG & Ahlstrand GG. 2003. Giant electron dense chains, clusters and granules in megakaryocytes and platelets with normal dense bodies: An inherited thrombocytopenic disorder III. Platelet analytical electron microscopy. Platelets 14(5) 305-312.
    • Blanchette, R.A., 2003. Deterioration in Historic and Archaeological Woods from Terrestrial Sites. In: Koestler RJ, Koestler, VR, Charola AE & Nieto-Fernandez FE, (Eds.), Art, Biology, and Conservation: Biodeterioration of Works of Art. The Metropolitan Museum of Art, New York, 328-347.
    • Held BW, Blanchette RA, Jurgens JA, Duncan S & Farrell RL. 2003 Deterioration and Conservation Issues Associated with Antarctica's Historic Huts. In: Koestler RJ, Koestler, VR, Charola AE & Nieto-Fernandez FE, (Eds.), Art, Biology, and Conservation: Biodeterioration of Works of Art. The Metropolitan Museum of Art, New York, 370-389.
    • Jurgens JA, Blanchette RA & Carlson DN. 2003. Evaluating the Wooden Remnants of the Tekta Burnu Shipwreck. In: Koestler RJ, Koestler, VR, Charola AE & Nieto-Fernandez FE, (Eds.), Art, Biology, and Conservation: Biodeterioration of Works of Art. The Metropolitan Museum of Art, New York, 390-409.
    • Held BW, Thwaites JM, Farrell RL & Blanchette, RA. 2003. Albino strains of Ophiostoma species for biological control of sapstaining fungi. Holzforschung 57:237-242.
    • Josephsen GD, Josephsen KA, Beilman GJ, Taylor J & Mulier KE. 2003 Use of microwave processing in the analysis of mitrochondrial ultrastructure injury. Proceedings, Microscopy and Microanalysis, San Antonio, TX.
    • Marks MD.& Esch JJ. 2003. Initiating inhibition: Control of epidermal cell patterning in plants. EMBO reports 4:24-25
    • Bushnell WR, Seeland TM, Perkins-Veasie P, Krueger DE, Collins J & Russo, VM. 2003. Calcium ions increase toxicity of deoxynivalenol to barley leaf tissues. 2003 National Fusarium Head Blight Forum, Proceedings. Minneapolis Minnesota, December 13-15.
    • White JG & Ahlstrand GG. 2003. Giant electron dense chains, clusters and granules in megakaryocytes and platelets with normal dense bodies: An inherited thrombocytopenic disorder IV. Ultrastructural cytochemistry and analytical electron microscopy. Platelets 14(5), 313-324.
    • Esch JJ, Chen M, Sanders MA, Hillestad M, Ndkium S, Idelkope B, Neizer J & Marks, MD. (2003) A contradictory GLABRA3 allele helps define gene interactions controlling trichome development in Arabidopsis. Development 130:5885-5894.
    • Zeyen RJ, Kruger WM, Lyngjaer MF & Carver TLW. 2002. Differential effects of D-mannose and 2-deoxy-D-glucose on attempted powdery mildew infection of inappropriate and appropriate Gramineae. Physiological and Molecular Plant Pathology 61:315-323.
    • Bushnell WR, Hazen BE & Pritsch C. 2003. Histology and physiology of Fusarium head blight. In: Leonard KJ & Bushnell WR, (Eds.), Fusarium Head Blight of Wheat and Barley, pp 44-93, APS Press, St. Paul.
    • Falbel TG, Koch LM, Nadeau JA,Segui-Simarro JM, Sack FD & Bednarek SY. 2003. SCD1 is required for cytokinesis and polarized cell expansion in Arabidopsis thaliana. Development 130, 4011-4024.
    • Gonda K, Fowler J, Katoku-Kikyo N, Haroldson J, Wudel J & Kikyo N. 2003 Reversible disassembly of somatic nucleoli by the germ cell proteins FRGY2a and FRGY2b. Nature Cell Biology 5, 205-210.
    • Lyte M, Neal CP, Olson BA, Parkin JL, Freestone PPE, Haigh RD, Bayston R & Williams PH. 2003. Stimulation of Staphylococcus epidermidis growth and biofilm formation by catecholamine inotropes. The Lancet 361:130-135.
    • Jordan B. 2003. Analysis of environmental conditions and types of biodeterioration affecting the preservation of archaeological wood remains at the Kolding shipwreck site. PhD thesis, Advisor: E. Schmidt. University of Minnesota, St. Paul.
    • Wang GJ, Jackson JG. & Thayer SA. 2003. Altered distribution of mitochondria impairs calcium homeostasis in rat hippocampal neurons in culture. J. Neurochem. 87: 85-94.
    • Lockhart BEL. 2002. Differential response of hosta cultivars to infection by hosta virus X potexvirus - a basis for disease management. Acta Hort. 568:69-72.
    • Lockhart BEL & Geering AD. 2002. Partial characterization of two aphid-transmitted viruses associated with yellow leafspot of Spiraea. Acta Hort. 568:163-168.
    • Lockhart BEL, Fetzer J & Westendorp J. 2002. Previously unreported viral diseases of Aster Heuchera, Lobelia, Pulmonaria and Physostegia in the USA. Acta Hort. 568:221-224.
    • Harper G, Hull R, Lockhart BEL & Olszewski N. 2002. Viral sequences integrated into plant genomes. Ann. Rev. Phytopathol. 40:119-136.
    • Jones AT, McGavin WJ, Geering ADW & Lockhart BEL. 2002. Identification of Rubus yellow net virus as a distinct badnavirus and its detection by PCR in Rubus species and in aphids. Ann. Appl. Biol. 141:1-10.


    Progress 01/01/02 to 12/31/02

    Outputs
    Total hours used:Electron microscopes,x-ray microanalysis equipment,cryo-stage;includescalibration,service,class demonstrations&tours.1167.75hours.PROJECT LEADERS USERS.DEPTS OF COLLEGE OF AG FOOD & ENV SCI MAES 402.25h.AGRONOMY & PLANT GENETICS16.5Project Leader: Muehlbauer.Users:Otto, Kruger, Alsop.ENTOMOLOGY34.25 Project Leaders:Kurtti,Munderloh,Hansen.Users:Palmer, Munderloh,Hansen.FOOD SCIENCE&NUTRITION2.5Project Leader:Reineccius.Users:Luo.HORTICULTURAL SCIENCE 22.25Project Leaders:Gillman,A.Smith,Thill.Users:J.Smith, A. Smith,Westberg,Dinu.PLANT PATHOLOGY326.75.Project Leaders:Zeyen,Blanchette,Lockhart,Gould,Samac,Bushnell. Users:Ahlstrand,Zeyen,Lockhart,Castell,Mogen,Bushnell,Krueger, Jurgens,Held,Freeland,J.Smith.DEPTS OF COLLEGE OF BIOLOGICAL SCIENCES54.25BIOCHEM,MOLBIO, BIOPHYS 9.5ProjectLeaders:Das,Lovrien.Users: Kumar,Ahlstrand,Judd, Raulinaitis.B.T.I.19.0ProjectLeaders:Wackett,Fickinger,Srienc; Users:Dodge,Nagarajan,Pederson.GENETICS CELL BIODEV 19.25ProjectLeader:Silflow,Neufeld,Levebvre.User:Silflow, Iyadurai,Hennig,Cygnar.PLANT BIOLOGY 6.5Project Leaders: Marks,Weiblen;Users:Marks,Weiblen.OTHER UM DEPARTMENTS 134.75CHEMENG MATSC.I3.5ProjectLeader:Hu.User:Gatti. CLINICAL&POP SCIENCES20.5Project Leaders:Pijoan,Ames. Users:Lopes,Cardona,Al-Ghamdi.FISHERIES WILDLIFE CONSBIO9.5Project Leader:Hove. Users: Hove,Whitney.LAB MED &PATHOLOGY4.25ProjectLeaders:J.White,Mescher.Users:White, Ahlstrand,Bell.MECH ENG13.0ProjectLeaders:Zachariah,Girshick. Users:Miller,Ahlstrand,Raj,Zhang.MEDICINE 4.5Project Leader: Kikyo.User:Kikyo.PEDIATRICS26.75ProjectLeaders:Gale,Godfrey. Users:Berman,Palmer,Ahlstrand,McClellan.SURGERY14.5Project Leader:Beilman.User:JosephsenVET DIAG LAB0.5Project Leader: Wunschmann.User:K.Josephsen.VETPATHOBIO26.75Project Leader:Gebhart.User:Guedes.WOOD&PAPERSCIENCE 11.5Project LeaderSchmidt.User:Jordan.OUTSIDE UNIVERSITIES & COLLEGES48.25Universidad del Bio-Bio7.0Navarrete, Ahlstrand.WinonaState2.75Thompson,Sullivan, Macalester College0.75Aanonsen,Discotte,ClemsonUniversity8.0Wourms, Krueger,U.Wisconsin,RiverFalls12.0Mogen,U.Wisconsin,Madison 17.75Falbel,McManus,Best,Ahlstrand.NON-UNIVERSITY USERS 149.75.Medtronic3.5Hefelfinger,Ahlstrand,Krueger,Robinson, PhillipsPlastics33.25Fitch,Entezarian,Hoopman,Ahlstrand, AmericanPetrographics6.75Edwards,Wolter,SIMS Deltec22 D. Johnson,Cargill12.5Piering,McGoogan,Ahlstrand,Gel-DelTech 10.75Masters,Ahlstrand, Intra-Therapeutics2.5Vreeman, Moberg,Surmodics7.25Wormuth,McKaren,McKay),Spineology (7.75 Wolfe), MN CardiovascularResInst 3Jorgenson,Schwartz, Tru-Fresh Marketing40.5Lipman,Ahlstrand.CLASSES & TOURS 44.5.Project Leaders:MacDonald,Percich,Elde(tours),Sanders,J. Phillips,R.Phillips,Johnston(Bethel College).INSTRUMENT CALIBRATIONS & MAINTENENCE(333.0)Imaging Center ,117Ahlstrand. F.E.I. SERVICE 94Olsen.HITACHI INSTRUMENTS SERVICE 72Griffin).EDAX,Inc50Swada.

    Impacts
    The project supports the electron optical imaging and X-ray microanalysis needs of researchers employed by the Minnesota Agricultural Experiment Station. Instrumentation in the facility was purchased by grants from the National Science Foundation, from MAES funds and from funds available from the Graduate School of the University of Minnesota and the College of Biological Sciences. To augment Electron Optical Facility finances for operation, excess instrument time is made available for a fee to other University of Minnesota research programs and to local agribusiness, biomedical and engineering businesses.

    Publications

    • Nasr, N. 2002. Aspects of Immunity in Mosquito Cell Culture. Ph.D. thesis, advisor, A. Fallon. University of Minnesota, St. Paul.
    • Prather, A.L. 2002. Phylogenetic analyses of Leptoceroidea and Calamoceratidae and revisions of the Neotropical genera Banyallarga and Phylloicus (Insecta: Trichoptera). Ph.D. thesis, advisor, R.W. Holzenthal. University of Minnesota, St. Paul.
    • Kruger, W.M., T.L.W. Carver & R.J. Zeyen. 2002. Effects of inhibiting phenolic biosynthesis on penetration resistance of barley isolines containing seven powdery mildew resistance genes or alleles. Physiological and Molecular Plant Pathology 61(1):41-51.
    • Danesh, D, N. Shekhawat, G. Cardinet, P. Thoquet, J. Mudge, S. Penuela, D. Kim, G. Kiss, H. Choi, E. Limpens, R.J. Zeyen, T. Huguet, D.R. Cook & N.D. Young. 2001. Integrated Microsatellite Mapping and Powdery Mildew Resistance in Medicago truncatula. 13th International Genome Sequencing and Analysis Conference, San Diego, CA.
    • Lyngkjaer, M.F., T.L.W. Carver & R.J. Zeyen. 2001. Virulent Blumeria graminis infection induces penetration susceptibility and suppresses race-specific hypersensitive resistance against avirulent attack in Mla1-barley. Physiological and Molecular Plant Pathology 59:243-256.
    • Zeyen, R.J., T.L.W. Carver & M.F. Lyngkjaer. 2002. Epidermal Cell Papillae. In: Belanger RR, Bushnell WR, Dik AJ, Carver TLW (eds). The Powdery Mildews: A Comprehensive Treatise. APS Press. pp. 107-125. St. Paul, Minnesota USA.
    • Zeyen, R.J. 2002. Silicon in Plant Cell Defenses Against Cereal Powdery Mildew Disease. In: Matoh T., J.F. Ma & E. Takashashi (eds). Silicon in Agriculture: The 2nd International Conference on Silicon in Agriculture, The Japanese Society of Soil Science and Plant Nutrition. pp 15-21. Tsuruoka, Japan
    • Bushnell W.R., T.M. Seeland & D.E. Krueger. 2002. Light-dependent bleaching of detached barley leaf tissue by deoxynivalenol. Phytopathology. 92:S11.
    • Seeland T.M., W.R. Bushnell & D.E. Krueger. 2002. Effects of deoxynivvalenol on detached barley leaf segments. Proc. North American Barley Researchers Workshop, Fargo ND
    • Kurtti T.J., A.T. Palmer & J.H. Oliver, Jr. 2002. Rickettsiella-like bacteria in Ixodes woodi (Acari: Ixodidae). J. Med. Entomol. 39(3):534-540.
    • Jian L.C., J.M. Deng & P.H. Li. 2002. Seasonal alteration of the cytosolic and nuclear Ca concentrations in overwintering woody and herbaceous perennials in relation to the development of dormancy and cold hardiness. J. Am. Hort. Sci., 127(6).
    • Ruiz A., L. Galina & C. Pijoan. 2002. Mycoplasma hyopneumoniae colonization of piglets sired from different boars. Canadian journal of veterinary research. Vol 66:79-85.
    • Johnson, R.G., A. Meyer, X-R. Li, D.M. Preus, L. Tan, H. Grunenwald, A.F. Paulson, D.W. Laird & J.D. Sheridan. 2002. Gap junctions assemble in the presence of cytoskeletal inhibitors, but enhanced assembly requires microtubules. Exp. Cell Res. 275:67-80.
    • Marques, G., H. Bao, T.E. Haerry, J.J. Shimell, P. Duchek, B. Zhang & M.B. O'Connor. 2002. The Drosophila BMP Type II receptor wishful thinking regulates neuromuscular synapse morphology and function. Neuron. Vol. 33, 529-543.
    • Buffo, R.A., K. Probst, G. Zehentbauer, Z. Luo & G.A. Reineccius. 2002. Effects of agglomeration on the properties of spray-dried encapsulated flavors. Flavour Fragrance J. 17:292-299.
    • Finney, J., R. Buffo & G.A. Reineccius. 2002. Effects of type of atomization and processing temperatures on the physical properties of spray-dried flavors. J. Food Science. 67(3):1108-1114.
    • Ronald, P.S. & R.G. St-Pierre. 2002. Infection process of Entomosporium mespili on leaves of saskatoon, Amelanchier alnifolia. Can. J. Plant Pathol. 24:363-371.
    • Andersen, A.W., C.B.S. Tong & D.E. Krueger. 2002. Comparison of periderm color and anthocyanins of four red potato varieties. American Journal of Potato Research. 79: 249-253.


    Progress 01/01/01 to 12/31/01

    Outputs
    Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, service and class demonstrations/tours. (1174.0) hours. PROJECT LEADERS & USERS Depts. of College of Ag., Food. & Env. Sci., MAES ENTOMOLOGY(99.0) - Project Leaders: Kurtti, Munderloh, Holzenthal, Seybold. Users: Palmer, Prather, Kyhl. FISHERIES, WILDLIFE & CONS. BIO.(6.0) - Project Leader: Hove. User: Hove. FOOD SCIENCE & NUTRITION(15.5) - Project Leader: Reineccius, O'Sullivan. Users: Luo, Grosso, Ajmila. HORTICULTURAL SCIENCE(86.75) - Project Leaders: Li, Gillman, Smith. Users:J. Smith, Jian, Deng, Smith, Graham. PLANT PATHOLOGY(152.0.) - Project Leaders: Zeyen, Blanchette, Lockhart, Gould, Samac, Bushnell, Groth, Kinkle. Users: Ahlstrand, Zeyen, Lockhart,Castell, Danesh, Bushnell, Krueger, Jurgens, Held, Freeland, Hilburn, Tamar,S. Lee. WOOD & PAPER SCIENCE(6.0) - Project Leader: Schmidt. User: Jordan. Other University Departments ANTHROPOLOGY(31.25) - Project Leader - Tappen. Users: Soderberg, Russell. BIOCHEMISTRY(22.0) - Project Leaders: Das, Bernlohr, Krick. Users: Kumar,Ahlstrand, Judd, Hertzel, Higgins. CLINICAL & POP. SCIENCES(59.75) Project Leader: Pijoan. Users: Ruitz, Utrera, Oliveira, Lopes, Cardona. GENETICS CELL BIO. DEV.(91.75) - Project Leader: O'Connor, Silflow, Hays.User: Marques, Silflow, Iyadurai, Krueger, Mingang. B.P.T.I.(50.5) - Project Leaders: Wackett, Fickinger; Users: Solheid, Nagarajan, Sepp, Strong, Tony. LAB MED & PATHOLOGY(2.5) - Project Leader: J. White. User: White. PEDIATRICS(0.75) - Project Leader:Ingbar. User: Jung. PHARMACOLOGY(6.75) - Project Leader: Thayer. User: Wang. PLANT BIOLOGY (38.25) - Project Leader: Marks, Olszewski; User: Marks, Sattler, Chen, Hartweck. SURGERY(39.75) - Project Leader: Lyte. User: Parkin. NON-UNIVERSITY USERS(157.5) - Medtronic (Krueger, Thompson, Coscio), Phillips Plastics (Fitch, Entezarian.), American Petrographics (Tilema, Wolter), Deltec.(Dan Johnson), Aveda (Modert), Hitachi(Teppert), Cargill(Ulku, Zidwick, Ahlstrand, Sanders), Abbott Hospital(Maselis, Evenson), Intra-Therapeutics(Vreeman, Gracek, Klima), Land O' Lakes, A.D.C.(Wroge). CLASSES & TOURS (50.5)- Project Leaders - MacDonald, Percich, Elde(misc. tours), J. Phillips, Lockhart, Kurle, Iwanij, Bio-Colloquium, Jewish Middle School, KSTP-TV. INSTRUMENT CALIBRATIONS & MAINTENENCE(125.0) - Ahlstrand. F.E.I. SERVICE(47.5) - Olsen. HITACHI INSTRUMENTS SERVICE(85.0) - Griffin.

    Impacts
    The project supports the electron optical imaging and X-ray icroanalysis needs of researchers employed by the Minnesota Agricultural Experiment Station. Instrumentation in the facility was purchased by grants from the National Science Foundation, from MAES funds and from funds available from the Graduate School of the University of Minnesota. To augment Electron Optical Facility finances for operation, excess instrument time is made available for a fee to other University of Minnesota research programs and to local agribusiness, biomedical and engineering businesses.

    Publications

    • Seeland, T. and W.R. Bushnell, 2001. Effects of deoxynivalenol on barley leaf pigmentation. pp. 151-153 , In: Proceedings 2001 National Fusarium Head Blight Forum. Erlanger KY, December 8-10,2001.
    • Ewing, H. 2000. Ecosystem development and response to climatic change: a comparative study of forest-lake ecosystems on different substrates. Ph.D.thesis, E. Nater, advisor. University of Minnesota, St. Paul.
    • Silflow, C.D., M. LaVoie, S. Tousey, W. Wu, M. Borodovsky, and P.A. Lefebvre. 2001. The Vfl1 protien in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies. J.Cell Biology 153:63-74.
    • Brazelton,W.J., C.D. Amundsen, C.D. Silflow, P.A. Lefebvre. 2001. The bld1 mutation identifies the Chlamydomonas osm-6 homolog as a gene required for flagellar assembly. Current Biology 11:1591-1594.
    • Buffo, R.A. and Reineccius, G.A. 2001. Comparison among assorted drying processes for the encapsulation of flavors. Perfumer and Flavorist 26:58-67.
    • Controlled synthesis of polyhydroxyalkanoic (PHA) nanostructues in R. eutropha. 2001. Kelley, A.S., N. V. Mantzaris, P. Daoutidis, and F. Srienc.Nanoletters. Vol. 1, No. 9, pp. 481-485.
    • Kelley, A. 2000. Controlling polyhydroxyalkanoate (PHA) polymer microstructures synthesized by Ralstonia eutropha . Ph.D. thesis, advisor, F. Srienc. University of Minnesota, St. Paul.
    • Lockhart, B.E.L. 2000. Virus diseases of Musa in Africa: epidemiology, etection and control. Acta Hort. 540:355-359.
    • Rassaby, L., J-C. Girard, M.S. Irey, B.E.L. Lockhart and P. Rott. 1999. Survey of sugarcane yellow leaf syndrome in Reunion Island. Sugar Cane, 11-16, October 1999.
    • Lockhart, B.E., J. Menke, G.Dahal, and N.E. Olszewski. 2000. Characterization and genomic analysis of tobacco vein-clearing virus, a plant pararetrovirus that is transmitted vertically and related to sequences integrated in the host genome. Journal of General Virology 81:1570-1585.
    • Lockhart, B.E. and L. J-C. Autrey. 2000. Mild mosaic. In P. Rott et al(eds.). A Guide to Sugarcane Diseases. CIRAD/ISSCT, Montpellier, France, 349 pp.
    • Lockhart, B.E. and L. J-C. Autrey. 2000. Sugarcane bacilliform virus. In P. Rott et al (eds.). A Guide to Sugarcane Diseases. CIRAD/ISSCT, Montpellier, France, 349 pp.
    • Lockhart, B.E. 2000. Hosta viruses - a research update. Midwest Regional osta Society Newsletter, 47:3-5.
    • Scagliusi, S.M. and B.E.L. Lockhart. 2000. Transmission, characterization and serology of a luteovirus associated with yellow leaf syndrome of sugarcane. Phytopathology 90:120-124.
    • Smith, G.R., Z. Borg, B.E.L. Lockhart, K.S. Braithwaite and M.J. Gibbs. 2000. Sugarcane yellow leaf virus: a novel member of the Luteoviridae that probably arose by inter-species recombination. Journal of General Virology 81:1865-1869.
    • Ndowora, T.C.R. and B.E.L. Lockhart. 2000. Development of a serological assay for detecting serologically diverse banana streak virus isolates. Acta Hort. 540:377-388.
    • Dahal, G., R. Ortiz, A. Tenkouano, J.d'A. Hughes, G. Thottappilly, D. Vuylsteke and B.E.L. Lockhart. 2000. Relationship between natural occurrence of banana streak badnavirus and symptom expression, relative concentration of viral antigen, and yeild characteristics of some micropropagated Musa spp. Plant Pathology 49:68-79.
    • Pasberg-Gauhl, C., B.E. Lockhart and S. Duran. 2000. First outbreak of banana streak badnavirus infection in commercial export bananas in Costa Rica. Plant Disease 84;1152.
    • Lockhart, B.E.L. 2000 Dicentra, Epimedium and Heuchera: new perennial ornamental hosts of Tobacco rattle virus in the United States. Plant Disease 94:1344.
    • Lockhart, B.E. and B.K. Johnson. 2000. Studies on serological and biological diversity among isolates of rhizomania-related furoviruses of sugarbeets in Minnesota. Annual Reporting Session, Sugarbeet Research and Education Board of Minnesoat and North Dakota, Fargo, ND. January, 2000.
    • Avila, R., M.C. Arrieta, W. Villalobos, L. Moireira and B.E.L. Lockhart. 2001. First report of Sugarcane yellow leaf virus (SCYLV) in Costa Rica. Plant Disease 85:919.
    • Jones, A.T., W.J. McGavin, A.D.W. Geering and B.E.L. Lockhart. 2001. A new badnavirus in Ribes species, its detection by PCR, and its close association with gooseberry vein banding disease. Plant Disease 85:417-422.
    • Chatenet, M., C. Delage, M. Ripolles, M. Irey, B.E.L. Lockhart and P. Rott. 2001. Detection of Sugarcane yellow leaf virus in quarantine and production of virus-free sugarcane by apical meristem culture. Plant Disease 85:1177-1180.
    • Lockhart, B.E.L. 2001. A new virus-associated disease of corn in Minnesota. Phytopathology 91:556.
    • Jones, A.T., W.J. McGavin, A. Geering and B.E.L. Lockhart. 2001. Detection and relationships of two new badnaviruses from temperate berry fruit plants. Phytopathology 91:545.


    Progress 01/01/00 to 12/31/00

    Outputs
    Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, service and demonstrations -(1223.25) hours. AGRONOMY & PLANT GENETICS(20.5) - Project Leader: Muehlbauer. User: Babb. BIOSYSTEMS & AG ENGINEERING(3.0) - Project Leader: Ruan. User: Montenegro. ENTOMOLOGY(152.5) - Project Leaders: Kurtti, Munderloh, Spivak, Fallon, Seybold.Users: Palmer, Ahlstrand, Srinivas Chinta, Prather, Kyhl. FOOD SCIENCE & NUTRITION(23.5) - Project Leader: Reineccius. Users: Newman, Luo,Holton. HORTICULTURAL SCIENCE(115.75) - Project Leaders: Li, Tong, Smith. Users: Chen, McNeil, Li, Jian, Deng, Krueger, Smith. PLANT PATHOLOGY(157.25.) - Project Leaders: Zeyen, Blanchette, Lockhart, Gould,Samac, Groth. Users: Ahlstrand, Zeyen, Lockhart, Johnson, Ying, Krueger,Jurgens, Held, Purev, Hilburn, Baldridge. SOIL, WATER & CLIMATE(4.25) - Project Leader: Nater. User: Ewing. ANTHROPOLOGY(4.0) - Project Leader - Tennesen. User: Tennesen ANIMAL SCI. VET. MED.(53.5) - Project Leader: Pisoan. User: Ruiz. BIOCHEMISTRY(75.75) - Project Leaders: Bloomfield, Das, Hooper. Users: Kumar, Ahlstrand, Judd, Krueger, Whittaker, Schwinefus. CLINICAL & POP. STUDIES(4.75) Project Leader: Joo. User: Choi. ECOLOGY, EVO. & BEHAV(1.0) - Project Leader: Hobbie. User: McLauchlan. GENETICS CELL BIO. DEV.(9.75) - Project Leader: O'Connor. User: Marques. B.P.T.I. (25.75) - Project Leaders: Srienc, Fickinger; Users: Kelley, Sepp. MOL.CELL DEV. BIOLOGY & GENETICS.(91.75) - Project Leaders: Silflow, Lefebvre, Sanders, Parkin; Users: Silflow, Krueger, Bode, Tan, Parkin, Ahlstrand. MEDICAL SCHOOL(VA) (6.25) - Project Leader: Simon; User: Talarico. PLANT BIOLOGY (17.75) - Project Leader: Marks; User: Marks, Sattler, Lukens. SMALL ANIMAL CLINIC (1.25) - Project Leader: Osbourne; User: Ulrich, Ahlstrand. Medtronic (Krueger, Thompson, Robinson), Microscpy Consulting Services (Schwach), Phillips Plastics (Wolfe, Fitch, Entezarian, Ko),American Petrographics (Tilema, Wolter), Deltec.(Dan Johnson), Aveda (Russell,Johnson). Project Leaders - MacDonald, Percich, Mogen, Miller, R. Phillips, Lockhart, Muehlbauer, Slocum, J. Phillips, Sanders, Zeyen. INSTRUMENT CALIBRATIONS & MAINTENENCE(96.5) - Ahlstrand, Zeyen. PHILIPS ELECTRONICS INSTRUMENTS SERVICE(243.5) - Olsen.

    Impacts
    The project supports the electron optics and X-ray microanalysis needs needs of researchers employed by the Minnesota Agricultural Experiment Station. Instrumentation in the facility was purcashed by grants from the National Science Foundation, from MAES funds and from funds avialable from the Graduate School o f the University of Minnesota To agument Electron Optical Facility finances for operation, excess instrument time is made available for a fee to other University of Minnesota research programs and to local agribusiness, biomedical, and engineering businesses.

    Publications

    • R. J. Zeyen, G.D. Baldridge, W.R. Bushnell, K.L.B. Hilburn. 2000. A Microassay Approach to Rapid Antifungal Protein Gene Pretesting. National Fusarium Headblight Forum -2000. Cincinnati Ohio, Dec. 10-12. 4 pp.
    • Hilburn KLB, Bushnell WR, Baldridge GD, & Zeyen RJ. 2000. Toward a plant suspension cell assay for eukaryotic antifungal protein constructs used in cereal transformation. Phytopathology 90(6): S35.
    • Hilburn KLB, Baldridge GD, Bushnell WR, Zeyen RJ. 2000. A Visible Fungal Growth Approach to Rapid Antifungal Protein Gene Pretesting. National Fusarium Headblight Forum 2000. Cincinnati Ohio, Dec. 10-12. 4 pp.
    • Smith, L, M. Wyckoff, G. Baldridge, R. Zeyen and G.J. Muehlbauer. 2000 Antifungal protein gene expression in transgenic wheat (Triticum aestivum). Agronomy Society of America Abstracts
    • Saruul, P., D.A. Somers and D.A.Samac. 2000. Synthesis of biodegradable plastics in alfalfa plants. Proceedings of the 37th North American Alfalfa Improvement Conference. p.206.
    • Kumar, R., Y.-H Xie and A. Das. 2000. Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore. Molecular Microbiology 36:608-617.
    • Jian, L-C, J.H. Li, P.H. Li and T.H. Chen. 2000. Structural association of ER other membrane systems in poplar apical bud cells and its alterations during the short-day-induced dormancy. Acta Boanica Sinica 42:803-810.
    • Jian, L-C, J.H. Li and P.H. Li. 2000. Seasonal alteration in amount of Ca in apical bud cells of mulberry: an electron microscopy-cytochemical study. Tree Physiology 20:623-628.
    • Jian, L-C, J.H. Li, P.H. Li and T.H. Chen. 2000. An electron microscopic-cytochemical localization of plasma membrane CaATPase activity in poplar apical bud cells during the induction of dormancy by short-day photoperiod. Cell Research 10:103-114.
    • Chen, W-P. 2000. Regulation of calcium onflux, free radical production and alternative pathway activity is associated with ABA-improved chilling tolerance in maize cultured cells. Ph.D. thesis, P.H. Li, advisor. University of Minnesota, St. Paul. 191p.
    • Schoenbeck, M.S., S.J. Temple, etal. 2000. Decreased NAOH glutamate synthaseactivity in nodules and flowers of alfalfa transformed with... J. Experimental Botany 51:29-39.
    • Schoenbeck, M.A. 1997. Physiological and molecular analyses of two genes expressed in alfalfa root nodules. Ph.D. thesis, C. Vance, advisor. University of Minnesota, St. Paul.
    • Tappen, M., and R Wrangham. 2000. Recognizing hominoid-modified bones: The taphonomy of Red Colobus bones digested by free-ranging chimpanzees in the Kibale Forest, Uganda. American Journal of Physical Anthropology 113: 217-234.
    • Illyes, G., P.J. Talarico and G. Simon. 2000. Different structural vascular changes in angiotensin II-treated and cold-stressed rats. American Journal of Hypertension 13:802-809.
    • Dahal, G., F. Gauhl, C. Pasberg-Gauhl, J.d'A. Hughes, G. Thottappilly and,B.E.L. Lockhart. 1999. Evaluation of micropropagated plantain and banana (Musa spp.) for banana streak badnavirus incidence under field and screenhouse conditions in Nigeria. Annals of Applied Biology 134:181-191.
    • Ndowora, T., G. Dahal, D. LaFleur, G. Harper, R. Hull, N.E. Olszewski, and B.E.L. Lockhart. 1999. Evidence that badnavirus infection in Musa can originate from integrated pararetroviral sequences. Virology 255:214-220.
    • Lockhart, B.E.L., and J. Lachner. 1999 An aphid-transmitted badnavirus associated with yellow leafspot of spiraea. Phytopathology 89:546.
    • Daugrois, J.H., I. Jean-Baptiste, B.E.L. Lockhart, M. Irey, and P. Rott. 1999. First report of sugarcane yellow leaf virus in the French West Indies. Plant Disease 83:588.


    Progress 01/01/99 to 12/31/99

    Outputs
    Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, service and demonstrations - (1,084.75) hours. DEPARTMENT, PROJECT LEADERS & USERS AGRONOMY & PLANT GENETICS(10.0) - Project Leader: Sommers. User: Oltoff. ENTOMOLOGY(45.0) - Project Leaders: Kurtti, Munderloh, Spivak, Holzenthal, Seybold. Users: Palmer, Ahlstrand, Srinivas Chinta, Prather, Kyhl. FOOD SCIENCE & NUTRITION(10.75) - Project Leaders: Srini, Reineccius. Users: Newman, Lu, Krueger. HORTICULTURAL SCIENCE(36.75) - Project Leaders: Li, Tong, Smith, Markhart. Users: Wen-Ping, McNeil, Li, Jian, De, Krueger, Hatch, Felsheim. PLANT PATHOLOGY(371.0) - Project Leaders: Zeyen, Bushnell, Blanchette, Lockhart, Gould, Samac, Groth. Users: Ahlstrand, Zeyen, Haight, Ronald, Lockhart, Johnson, Li Ying, Krueger, Jurgens, Purev, Castell. SOIL, WATER & CLIMATE(6.0) - Project Leaders: Nater. Users: Ewing.

    Impacts
    This Facility serves the needs of all Minnesota Agricultural Experiment Station researchers. Electron microscopes allow researchers to see objects 1000 times smaller than can be seen with the best light microscopes. These instruments are vital to many modern agricultural research programs.

    Publications

    • 5. Dahal, G., F. Gauhl, C. Pasberg-Gauhl, J.d'A. Hughes, G. Thottappilly and, B.E.L. Lockhart. 1999. Evaluation of micropropagated plantain and banana (Musa spp.) for banana streak badnavirus incidence under field and screenhouse conditions in Nigeria. Annals of Applied Biology 134:181-191.
    • 1. Bushnell, W.R., D.A. Somers, R.W. Giroux, L.J. Szabo, and R.J. Zeyen. 1998. Genetic engineering of disease resistance in cereals. Canadian Journal of Plant Pathology 20(2):137-220.
    • 2. Trepp, G.B., D.W. Plank, J.S. Gantt, and C.P. Vance. 1999. NADH-glutaurate synthase in alfalfa root nodules: Immunocytochemical localization. Plant Physiology 119:829-837.
    • 3. Gauhl, F., C. Pasberg-Gauhl, B.E.L. Lockhart, v, and G. Dahal. 1999. Incidence and distribution of banana streak badnavirus in the plantain production region of southern Nigeria. International Journal of Pest Management 45:167-171.
    • 4. Krikorian, A.D., H. Irizarry, R. Goenaga, M.E. Scott, and B.E.L. Lockhart. 1999. Stability in plant and bunch traits of a 'French-type' dwarf plantain micropropagated from the floral axis tip and five lateral corn tips of a single mother plant: Good news on the tissue culture and bad news on banana streak virus. Scientia Horticulturae 81:159-177.
    • 8. Daugrois, J.H., I. Jean-Baptiste, B.E.L. Lockhart, M. Irey, and P. Rott. 1999. First report of sugarcane yellow leaf virus in the French West Indies. Plant Disease 83:588.
    • 6. Ndowora, T., G. Dahal, D. LaFleur, G. Harper, R. Hull, N.E. Olszewski, and B.E.L. Lockhart. 1999. Evidence that badnavirus infection in Musa can originate from integrated pararetroviral sequences. Virology 255:214-220.
    • 7. Lockhart, B.E.L., and J. Lachner. 1999 An aphid-transmitted badnavirus associated with yellow leafspot of spiraea. Phytopathology 89:546.
    • 9. Trepp, G. 1999. NADH-dependent glutaurate synthase(NADH-GOGAT) and nitrogen fixation in alfalfa root nodules. Ph.D. thesis,.C.P. Vance, advisor. University of Minnesota, St. Paul. Nickolas Amrhein, advisor, Swiss Federal Technical Institute, Zurich, Switzerland.
    • 10. Cerrate-Reinoso, L. 1999. Characterization of three 'uni' mutations in Chlamydomonas reinhardtii. M.S. thesis, Carolyn D. Silflow, advisor. University of Minnesota, St. Paul. 87p.
    • 11. Palmer, A.T., U.G. Munderloh, and T.J. Kurtti. 1999. Infection of tick cells by two nonpathogenic strains of Rickettsia. Microscopy & Microanalysis (Proceedings) 5(2)1140-1141.
    • 12. Munderloh, U.G., S.D Jauron, V. Fingerle, L. Leitritz, S.F. Hayes, J.M. Hautman, C.M Nelson, B. Huberty, T.J Kurtti, G.G. Ahlstrand, B. Greig, M.A. Mellencamp, and J.L. Goodman. 1999. Invasion and intracellular developement of the HGE agent in tick cell culture. Journal of Clinical Microbiology 37:2518-2524.
    • 13. Jian, L.-c., J.-h. Li, W.-P. Chen, P.H. Li, and G.G. Ahlstrand. 1999. Cytochemical localization of calcium and Ca+2-ATPase activity in plant cells under chilling stress: a comparative study between the chilling-sensitive Maize and the chilling-insensitive winter wheat. Plant Cell Physiology 40(10):1061-1071.
    • 14. Tong, C., D. Krueger, Z. Vickers, D. Bedford, J. Luby, and A. El-Shiekh. 1999. Comparison of softening-related changes during storage of 'Honeycrisp' apple, its parents, and 'Delicious'. J. Amer. Soc. Hort. Sci. 124(4):407-415.
    • 15. Sim, S. 1996. Inhibition of blackspot in commercial potatoes (Solanum tuberosum) and the biochemical basis for lack of enzymatic browning in wild potato (Solanum hjertingii). M.S. Thesis, C. Tong, advisor. University of Minnesota, St. Paul. 94 p.
    • 16. Pritsch, C. 1999. Early molecular responses of wheat spikes to Fusarium graminearum infection and histology of the infection process. Ph.D. Thesis,D. Sommers, advisor, University of Minnesota, St. Paul. 130 p.
    • 17. Szymanski, D.B., M.D. Marks, and S. M. Wick. 1999. Organized F-Actin is essential for normal thrchome morphogenesis in Arabidopsis. Plant Cell 11, 2331-2347.


    Progress 01/01/98 to 12/31/98

    Outputs
    Total hours used, electron microscopes, x-ray microanalysis equipment- 1115.25hours. DEPARTMENT, PROJECT LEADERS & USERS AGRONOMY & PLANT GENETICS(57.0) - Project Leaders: Vance, Muehlbauer. Users: Trepp, Pritsch, Muehlbauer. ENTOMOLOGY(112.5) - Project Leaders: Subramanyam, Kurtti. Users: Palmer, Ahlstrand, Krueger. FOOD SCIENCE & NUTRITION(18.0) - Project Leaders:Singh, Reineccius., Manote, Tatini,Smith. Users: Newman, Kauppi, Mauer, Singh, Krueger. HORTICULTURAL SCIENCE(37.0) - Project Leaders: Li, Tong, Smith, Carter. Users: Wen-Ping, McNeil, Li, Olmann, Krueger, Zigang. PLANT PATHOLOGY(230.5) - Project Leaders: Zeyen, Bushnell, Blanchette, Lockhart,Gould, Remah, Krupa. Users: Ahlstrand, Zeyen, Haight, Remah, Lockhart, Johnson, Mercier, Krueger, Scagliusi. SOIL, WATER & CLIMATE(116.25) - Project Leaders: Nater. Users: Nater, Ewing, Fleck. OTHER UNIVERSITY DEPARTMENTS ANIMAL SCIENCE(2.0) - Project Leader: Joo; Users: Shin. ANTHROPOLOGY(5.25) - Project Leader: TAPPEN; Users: Tappen, Erick. BIOCHEMISTRY(47.5) - Project Leaders: Bloomfield, Das; Users: Kumar, Deng. CELL BIOLOGY & NEUROANATOMY(9.25) - Project Leader: Porter; Users: Krueger, Rupp. CHEM. ENG. MAT. SCI.(1.5) - Project Leader: Tranquilo; Users: Tower, Girton. GENETICS & CELL BIO.(96.25) - Project Leaders: Silflow, Hays, Marks; Users: Bode, Marks, Silflow, Catron, Szymanski, Ahlstrand, Krueger. ENVIRON. & OCU. HEALTH(52.5) - Project Leader: Swackhamer; User: Jabusch. FISHERIES & WILDLIFE(3.25) - Project Leader: Spangler; User: Schrag. MEDICAL SCHOOL (29.0) - Project Leader: Simon; User: Illyes, Talarico. UM - DULUTH BIOLOGY (5.0) Project Leader: Liu; User: Sedgewick. NON-UNIVERSITY USERS(72.25) - Medtronic (Coscio, Ahlstrand, Fernandez, Krueger, Thompson, Schroder), General Mills (Lakkis), Metagen (Marx, Venarsky), Sandpiper Institute (Miller), Powder Metal Moulding (Tandon), Insecto, Inc. (Huber), Green Pastures (Powers), U. Wisconsin (Fly), Braun Intertech (Humenansky) Microscopy Consulting Services (Schwach), Phillips Plastics (Wolfe), American Petrographics (Tilema, Wolter). CLASSES & TOURS - Project Leaders(27.25) - MacDonald, Percich, R. Phillips, Lockhart, Meronuck, Bloom, Schwach. INSTRUMENT CALIBRATIONS & MAINTENENCE(65.5) - Ahlstrand, Zeyen. PHILIPS ELECTRONICS INSTRUMENTS SERVICE(127.5) - Olsen.

    Impacts
    (N/A)

    Publications

    • 8. Dahal, G., J.d'A. Hughes and B.E.L. Lockhart. 1998. Status of banana streak disease in Africa: problems and future research needs. Integrated Pest Management Reviews 3:85-97.
    • 3. Carver, T.L.W., B.J. Thomas, M.P. Robbins and R.J. Zeyen. 1998. Phenylalanine ammonia-lyase inhibition, autofluorescence, and localized accumulation of silicon, calcium and manganese in oat epidermis attacked by the powdery mildew fungus Blumeria graminis (DC) Speer. Physiological and Molecular Plant Pathology 52:223-243.
    • 4. Carver, T.L.W., M.P. Robbins, B.J. Thomas, K. Troth, N. Raistrick and R.J. Zeyen. 1998. Silicon deprivation enhances localized autofluorescent responses and phenylalanini ammonia-lyase activity in oat attacked by Blumeria granimis. Physiological and Molecular Plant Pathology 52:245-257.
    • 5. Jian, L.-C., J.-H. Li and P.-H. Li. 1997. Is Ca2+ homeostasis essential in the cold acclimation of winter wheat seedlings? Proceedings of the International Symposium on Cereal Adaptation to low temperature stress, Martonvasar, Hungary, June 2-4, 1997. P. 69-71.
    • 6. Munderloh, U.G., S.F. Hayes, J. Cummings and T.J. Kurtti. 1998. Microscopy of spotted fever rickettsia movement through tick cells. Microscopy and Microanalysis 4(2):115-121.
    • 7. Cheng, C.-P., I. Tzafrir, B.E.L. Lockhart and N.E. Olszewski. 1998. Tubules containing virions are present in plant tissues infected with Commelina yellow mottle badnavirus. Journal of General Virology, 79:925-929.
    • 9. Dahal, G., J.d'A. Hughes, G. Thottappilly and B.E.L. Lockhart. 1998. Effect of temperature on symptom expression and reliability of banana streak badnavirus detection in naturally infected plantain and banana (Musa spp.). Plant Disease 82(1):16-21.
    • 10. Thottappilly, G., G. Dahal and B.E.L. Lockhart. 1998. Studies on a Nigerian isolate of banana streak badnavirus: I. Purification and enzyme-linked immunosorbent assay. Annals of Applied Biology 132:253-261.
    • 11. Trepp, G. 1998. Biochemical and molecular regulation of NADH-glutaurate synthase. Ph.D. thesis,.C.P. Vance, advisor. University of Minnesota, St. Paul.
    • 12. Chen, Y.-R., E. Schmidt and K. Olsen. 1998. Effect of compression of green wood chips on conidial germination and colonization of a biopulping fungus Phanerochaete chrysosporium. Wood and Fiber Science 30(1):18-26.
    • 13. Chen, Y.-R. 1998. Studies on biological kraft pulping of compression-baled wood chips with lignin-degrading fungi. Ph.D. thesis. E. Schmidt, advisor.University of Minnesota, St. Paul. 114p.
    • 14. Baumann, C. 1998. Ionic effects on the elasticity of single DNA molecules. Ph.D. thesis. V. Bloomfield, advisor. University of Minnesota, St. Paul. 165p.
    • 15. Schnell, J., J. Berman and V. Bloomfield. 1998. Insertion of telomere repeat sequence decreases plasmid DNA condensation by cobalt (III) hexaammine. Biophysical Journal 74:1484-1491.
    • 16. Szymanski, D. B., R. A. Jilk, S. M. Pollock and M. D. Marks. 1998. Control of GL2 expression in Arabidopsis leaves and trichomes. Development 125: 1161-71.
    • 17. Szymanski, D. B., D. A. Klis, J. C. Larkin and M. D. Marks. 1998. Cot1: a regulator of Arabidopsis trichome initiation. Genetics 149: 565-77.
    • 18. Szymanski, D. B. and M. D. Marks. 1998. GLABROUS1 overexpression and TRIPTYCHON alter the cell cycle and trichome cell fate in Arabidopsis. Plant Cell 10: 2047-2062.
    • 1. Zeyen, R.J., W.M. Kruger and T.L.W. Carver. 1998. Barley powdery mildew resistance genes control two different cell death pathways. Phytopathology 88(9)S103.
    • 2. Kruger, W.M., R.J. Zeyen, L.J. Saab and A.G. Smith. 1998. Barley powdery mildew resistance genes exert differential temporal transcription control over two chitinase genes. Phytopathology 88(9) S50.


    Progress 01/01/97 to 12/31/97

    Outputs
    FOOD SCIENCE & NUTRITION(105.75) - Project Leaders: Zottola, Fulcher, Reineccius.Users: Schwach, Diran, Newman, Sherwin, Medin, Li. HORTICULTURE(139.0) - Project Leaders: Li, Tong, Smith. Users: Wen-Ping, McNeil,Li, Craig, Tong, Karen, Howe. PLANT PATHOLOGY(252.75) - Project Leaders: Zeyen, Bushnell,Blanchette, Lockhart, Gould, Samac, Krupa. Users: Ahlstrand, Zeyen, Haight, McBride, Lockhart, Tsi Tsi, Mercier, D. Krueger, Scagliusi.. SOIL SCIENCE(7.5) - Project Leaders: Nater, Rosen. Users: Nater, Rosen, Ahlstrand, Pennington. OTHER UNIVERSITY DEPARTMENTS BIOCHEMISTRY(64.5) - Project Leader: Bloomfield, Allewell; Users: Hong, Schell, Dashuang, Baumann, Ya Ha, Yukiko CELL BIOLOGY & NEUROANATOMY(33.5) - Project Leader: Porter; Users: Krueger, Rupp. CHEM. ENG. MAT. SCI.(19.25) - Project Leader: Tranquilo; Users: Tower, Girton. GENETICS & CELL BIO.(77.25) - Project Leaders: Silflow, Rougvie, Marks; Users: Sattlar, Ross, Marks, Silflow, Wei-Chien, Kent, Pam, Szymanski, Ahlstrand, Krueger ENVIRON. & OCU. HEALTH(17.0) - Project Leader: Swackhamer; User: Jabusch. MEDICAL SCHOOL (43.75) - Project Leader: Simon; User: Illyes. PHARMACY(7.25) - Project Leader: Rahman; Users: Aparna, Tasna. PLANT BIOLOGY(28.25) - Project Leaders: Doebley, Olszewski; Users: Lauter, Iris. SMALL ANIMAL CLINICAL SCIENCES(6.5) - Project Leader: Osborne; Users: Ulrich, Koehler. NON-UNIVERSITY USERS(36.75) - Medtronic (Coscio, Ahlstrand, Fernandez, Krueger),Osmonics (Mueller), Metagen (Marx, Venarsky), Macalaster College (Foreman), Powder Metal Moulding (Tandon),Microscpy Consulting Services (Schwach), Archer Daniels Midland (Subi), American Petrographics (Tilema, Wolter). CLASSES & TOURS - Project Leaders(33.5) - MacDonald, Percich, R. Phillips, Jane Phillips (Upward Bound), Danesh, Lockhart, Meronuk, Sanders, Zeyen, Nater,Blanchette, Century College (Nordsving). INSTRUMENT CALIBRATIONS & MAINTENENCE(94.0) - Ahlstrand, Zeyen. PHILIPS ELECTRONICS INSTRUMENTS SERVICE(136.5) - Olsen, Swada.

    Impacts
    (N/A)

    Publications

    • 2. Jian, L., P. H. Li, L. Sun & T. H. H. Chen. 1997. Alterations in ultrastructure and subcellular localization of Ca2+ in poplar apical bud cells during the induction of dormancy. Journal of Experimental Botany 48:1195-1207.
    • 3. Tzafrir, I., L. Ayala- Navarrete, B. E. L. Lockhart and N. E. Olszewski. 1997. The N-terminal portion of the 216-kDa polyprotein of Commelina yellow mottle badnavirus is required for virus movement but not for replication. Virology 232:359-368.
    • 4. Lockhart, B. E. L., K. Kiratiya-Angul, P. Jones, L. Eng, P. De Silva, N.E. Olszewski, N. Lockhart, N. Deema and J. Sangalang. 1997. Identification of Piper yellow mottle virus, a mealybug-transmitted badnavirus infecting Piper spp.in Southeast Asia. European Journal of Plant Pathology 103:303-311.
    • 5. Grisham, M. P., Y-B Pan, D. M. Burner, Q. Wei, J. L. Glynn, M. S. Irey and B.E. Lockhart. 1997. Yellow leaf syndrome in Louisiana. Abstract: International Society of Sugar Cane Technologists, Pathology and Molecular Biology Workshop,Umhlanga Rocks, South Africa.
    • 6. Irey, M. S., L. E. Baucum, K. S. Derrick, K. L. Manjunath and B. E. Lockhart. 1997. Abstract: International Society of Sugar Cane Technologists, Pathology and Molecular Biology Workshop, Umhlanga Rocks, South Africa.
    • 7. Scagliusi, S. M. and B. E. Lockhart. 1997. Transmission, characteristics and sereology of sugarcane yellow leaf luteovirus. Abstract: International Society of Sugar Cane Technologists, Pathology and Molecular Biology Workshop, Umhlanga Rocks, South Africa.
    • 8. Dahal, G., J. d'A. Hughes, G. Thottappilly and B E. L. Lockhart. 1997. Effect of temperature on symptom expression and detection of banana streak badnavirus in plantain and bananas. Phytopathology 87:S22.
    • 9. Ha, Y., E. C. Thiel and N. M. Allewell. 1997. Preliminary analysis of amphibian red cell M ferrotin in a novel tetragonal unit cell. Acta Crystalographica D53:513-523.
    • 10. McBride, M. J. 1997. Colonization and effect of disease suppressive Streptomyces on alfalfa growth and nodulation. M. S. thesis. D. A. Samac, advisor. University of Minnesota, St. Paul. 75pp.
    • 11. Lyngkjaer, M.F., T.L.W. Carver and R.J. Zeyen 1997. Suppression of resistance to Erysiphe graminis f.sp. hordei conferred by the mlo5 barley powdery mildew resistance gene. Physiological and Molecular Plant Pathology50:17-36.
    • 1. Dorweiler, J.E., and J. Doebley. 1997. Developmental analysis of Teosinte glume architecture1 : A key locus in the evolution of maize (Poaceae). American Journal of Botany 84(10):1313-1322.


    Progress 01/01/96 to 12/30/96

    Outputs
    Agro & Plt Genetics (18.5)-Proj.Leaders: Vance, Jones. Ento (13.75)-Proj. Leaders. Subramanyam, Kurtti. Food Science & Nutr. (66.0) - Proj. Leaders: Zottola, Fulcher, Asp. For. Res. (3.0)-Proj. Leader: Perry. Horticulture (112.75)-Proj. Leaders: Li, Tong, Smith, Markhart. Pl.Path. (280.25)-Proj. Leaders: Zeyen, Bushnell, Blanchette, Lockhart, Banttari, Roelfs, Gould, Young. Soil Sci. (27.75)-Proj. Leaders: Nater. Wood & Paper Sci. (11.25)-Proj Leaders: Schmidt. Biochem (119.25)-Proj. Leader: Bloomfield, Allewell; Chem. Eng. Mat. Sci (10.25)-Proj Leader: Hu, Tranquilo; Genetics & Cell Bio. (54.75)-Proj Leaders: Silflow, Lefebvre, Marks, Dentler, Hays; Environ. & Ocu. Health (6.25)-Proj Leader: Swackhamer; Med., Infectious Dis (2.0)-Proj Leader. Goodman; Pl. Bio. (27.5)-Proj leaders: Doebley, Olszewski; Small Animal Clinical Sci (2.25)-Proj. Leader: Osborne; Non-University Users (28.75) - Medtronic, Osmonics, Metagen, Powder Particle Moulding, Microscope Consulting Serv., Am. Petrographics, Macalester College; Classes & Tours - Proj. Leaders (18.5)-MacDonald, Percich, Phillips, Karen K. (UW), Wick, McGlaughlin, Lockhart, Meronuck, Elsayed; Instrument Calibrations & Maint. (101.25)-Ahlstrand, Zeyen; Philips Electronics Instr. Serv. (153.5) - Olsen, Schaub.

    Impacts
    (N/A)

    Publications

    • Ahlstrand, G.G. 1996. Low-temperature low-voltage scanning electron microscopy (LTLVSEM) of uncoated frozen biological materials: A simple alternative. Proceedings of Microscopy and Microanalysis, Minneapolis, MN, pp. 91-919.
    • Carver, T.L.W., Zhang, L., Zeyen, R.J., & Robbins, M.P. 1996. Phenolic biosynthesis inhibitors upress adult plant resistance to E. gaminis in oat at 20C and 10C. Physiological & Molecular Pl Path. 49:121-142.
    • Lyngkjaer, M.F., Carver, T.L.W., Zeyen, R.J. 1996. Suppressin of mlo5 powdery mildew resistance in barley. Proceedings of the 9th European & Mediteranean Cereal Rusts & Powdery Mildew Conf. pp. 2-6. 9/1996. Luthern. The Netherlands.
    • Cheng, C.P., B.E. Lockhart & N.E. Olszewski. 1996. The ORFI + II proteins of Commelina yellowmottle virus are virion-associated. Virology 223:263-271.
    • Rupp, O'Toole, Gardner, Mitchell, Porter. 1996. The sup-pf-2 mutations of Chlamydomonas alter the activity of the outer dynein arms by modificatin of the v-dynein heavy chain. J. Cell Biology 135:1-13.
    • Currier, S., & B.E.L. Lockhart. 1996. Characterization of a potexvirus infecting Hosta spp. Plant Disease 80:1040-1043.
    • Robinson, D.L., S.M. Pathirana, J.S. Gantt & C.P. Vance. 1996. Immunogold localization of nodule enhanced phosphoeuolpyruvate carboxylase on alfalfa. Plant Cell & Environment 19:602-608.


    Progress 01/01/95 to 12/30/95

    Outputs
    Total hr. used 1508.5 for TEM and SEM. Users and affiliations: Agronomy. (13.5) Users: Robinson, Padma, Mauro; Entomology (75.5) Users: Krueger, Munderloh, Maldridge, Ahlstrand; Food Sci & Nutr. (78.5) Users: Whorton, Dirran, Zhou, Mannie, Schwach; Forest Prod. (5.5) Users: Ahlstrand, Schmidt; Forest Resource (58.5) Users: Hafner, Wagy, Beth; Horticulture (131.5) Wen-Ping, Longhua, Sopheak, McNeil, Zong, Jian; PlPa (485.5) Users: Ahlstrand, TsiTsi, Yang, Ping, Zeyen, Haight, Lockhart, Roberts, Krueger. Scagliusi, Longhua, Burnes; Soil Sci (22.0). Users: Ahlstrand, Nater, Eyasu, Rosen; Other Univ Depts (272.5) PI's. Hu (Chem, Eng. Mat. Sci); Doebley, Olszewski (PlBio), Silflow, Marks (GCB), Maier (Civil & Min. Eng), Bloomfield (Biochem), Swackhamer (Environ. Ocu. Health), Berndt (Geo). Users: Dorweiler, Ping, Wei Chien, Larson, Larkin, Marks, Pfaff, Baumann, Schell, Haber, Hong, Paula, Wu, Julie, Shun. Non-Univ. (27.5) Medtronic, Osmonics, Solvay, Maple Hill Labs. Users: Coscio, Ahlstrand, Lundmark, Krueger, Rademacher, Tsang. Classes & Tours (29.5); Instr. Mainte. (99.5); Philips Instr. Service (209.0), Olsen, Schaub.

    Impacts
    (N/A)

    Publications


      Progress 01/01/94 to 12/30/94

      Outputs
      Total hrs used, electron microscopes, x-ray microanalysis equip; includes calibration, serv. and demonstrations - 1189.0 hrs. Agronomy/Plant Genetics (45.25)-Proj Leaders: Vance, Jones. Users: Robinson, Padma, Paulo, Mauro, Karen. Ento (27.0)-Proj Leaders: Subi, Fallon. Users: Krueger, Baldridge, Lockhart. Food Sci(57.75)-Proj Leaders: Davis, Zottola, Reineccius, Fulcher, Asp. Users: Wenbo, Xing, McIntyre, Schwach, Whorton. Forest Res (1.0)-Proj leaders: Schmidt. Horticulture (125.0)-Proj Leaders: Hoover, Lee, Tong, Smith. Users: Foulk, Wen-Ping, Wang, Chen, El-Shiek. Plant Pathology (496.0)-Proj Leaders: Zeyen, Bushnell, Blanchette, Lockhart. Users: Ahlstrand, Tsi Tsi, Yang, Ping, Zeyen, Haight, Krueger, Lockhart, Anikster, Roberts, D. Krueger. Soil Sci (50.25)-Proj Leaders: Nater, Sadowski. Users: D. Krueger, Ahlstrand, Zhaner, Nater, Eyasu, Zhang. Fish & Wild (1.5)-Proj Leader: Spangler. User: McClure. Other Univ Depts (85.75)-Proj Leaders: Flo Wu (Biochem. Eng.), Doebley (Plant Bio.), Gibbon (Anthropology), Dentler (GCB), Marks (GCB), Users: Maura, Dorweilier, Pennington, Marks, Pam, Oppenheiner, Ecker, Beth. Non-Univ Users (60.75) Lundmark (Maple Hill Labs), Coscio (Medtronic), Wolter (Am. Petrographics), Umbach (Campbells Soup), Ross, Rademacher (Osmonics), Melchior (BSI), Orffield (Stout State), Schwach. Users: D. Krueger, Ahlstrand, Gerard. Classes & Tours (30.0)-Proj Leaders: Meronuck, MacDonald, Percich, Phillips, Mogen (UW), Schwach(MN Sci Museum).

      Impacts
      (N/A)

      Publications


        Progress 01/01/93 to 12/30/93

        Outputs
        Total hours used, electron microscopes, x-ray microanalysis unit, plus calibration, service and demonstrations -1315.25 hours. DEPARTMENT, PROJECT LEADERS & USERS: AGRONOMY & PL. GENETICS(81.0) - Project Leaders: Vance, Somers. Users: Robinson, Trepp, Eschenlauer, D.Krueger; ENTOMOLOGY(111.5) - Project Leaders: Kurtti, Fallon, Subramanyam; Users: D. Krueger, Ahlstrand, Iwano, Swanson; FOOD SCIENCE & NUTRITION(143.0) - Project Leaders: Davis, Zottola, Reineccius, McKay, Users: Bolton, Wenbo, Xing, Ahlstrand, Schwach, Baldwin, Whorton; FOREST RESOURCES(7.5) - Project Leader: Troelstrup, Users: Troelstrup, Ahlstrand; HORTICULTURE(33.75) - Project Leaders: Carter, Tong, Smith, Users: Erickson, Ahlstrand, Chen, El-Shiek; PLANT PATHOLOGY(399.5) - Project Leaders: Zeyen, Bushnell, Blanchette, Lockhart, Banttari, Plant Disease Clinic; Users: Ahlstrand, Zeyen, Haight, Cease, G. Krueger, Yang, Blanchette, Lockhart, Sophie, Banttari, Gould, Roberts, D. Krueger; SOIL SCIENCE(51.5) - Project Leader: Nater,Sadowski, Users: Nater, Kolka, Eyasu, Thompson, Zhao; FISHERIES & WILDLIFE(1.0) - Project Leader: Spangler, User: McClure; OTHER UNIVERSITY DEPARTMENTS(50.0) - Project Leaders: Mooers(UMD), Seyfried(Geology), Lively(Mn. Geo. Survey), Doebley(GCB), Pellett(Mn. Landscape Arbor.), Users: Bulliss, Beaverson, Stevens, Lively, McNamara, Dorweiler, Thompson; NON-UNIVERSITY USERS(46.25) - Medtronic, Cargill, Berkley, Osmonics, American Petrographics, Ecolab, B.S.I.

        Impacts
        (N/A)

        Publications


          Progress 01/01/92 to 12/30/92

          Outputs
          #85 Progress Report 1992 - Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, service and demonstrations - 1,328 hours. AGRONOMY & PLANT GENETICS (73.0) - Project Leader: Vance. User: Robinson. ENTOMOLOGY (172.0) - Project Leaders: Kurtti, Fallon, Andow. Users: Krueger, Ahlstrand, Olson. FOOD SCIENCE & NUTRITION (133.5) - Project Leaders: Davis, Zottola, Reineccius. Users: Davis, Foong, Zhou, Bolton, Umbach, Sasahara, Schwach, Wenbo. FOREST RESOURCES (88.5) - Project Leader: Troelstrup. Users: Troelstrup, Ahlstrand. HORTICULTURE (29.5) - Project Leaders: Carter, Hackett, Hoover, Brenner. Users: Erickson, Ahlstrand, Ozga, Foulk. PLANT PATHOLOGY (262.5) - Project Leaders: Zeyen, Bushnell, Blanchette, Anderson, Lockhart, Banttari, Plant Disease Clinic. Users: Ahlstrand, Zeyen, Jiang, Haight, Blanchette, Lockhart, Ayala, Banttari, Gould, Roberts. SOIL SCIENCE (14.0) - Project Leader: Nater. Users: Nater, Eyasu, Kolka. OTHER UNIVERSITY DEPARTMENTS (27.5) - Project Leaders: Pruitt, Seyfried, Wright, Engstrom, Lively. Users: Brenda, Ding, Stevens, Buttram, Ahlstrand. NON-UNIVERSITY USERS (40.5) - Medtronic, Cargill, Schwach, Osmonics, American Petrographics, U. Wisconsin, Macalester Col. Users: Coscio, Schwach, Orfield, Ruebel, Domsteen, Maly, Wolter, Lukkarila. CLASSES & TOURS (22.0) - Project Leaders: MacDonald, Kuehn, Lockhart, Kennedy, Schmidt, MacIntosh, Vance. INSTRUMENT CALIBRATIONS 7 MAINTENANCE (84.0) - Ahlstrand, Zeyen.

          Impacts
          (N/A)

          Publications


            Progress 01/01/91 to 12/30/91

            Outputs
            Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, service and demonstrations - 1,034.5 hours. Agronomy & Plant Genetics - (85 hr) Project Leaders: Phillips, Vance. Users: Graham, Robinson. Entomology - (75.5 hr) Project Leaders: Kurtti, Fallon, Andow. Users: Krueger, Ross, Ahlstrand, Olson. Fisheries & Wildlife - (5.0 hr) Project Leader: Sorenson. User: Li. Food Science & Nutrition - (80.5 hr) Project Leaders: Davis, Asp, Zottola, Breen. Users: Davis, Rooney, Zhou, Holton, Sasahara, Perez. Forest Resources - (2.5 hr) Project Leader: Perry. User: Troelstrup. Horticulture - (117.5 hr) Project Leaders: Carter, Markhart. Users: Erickson, Harper. Plant Pathology - (255.5 hr) Project Leaders: Zeyen, Young, Blanchette, Anderson, Lockhart, Banttari, Plant Disease Clinic. Users: Ahlstrand, Zeyen, Jiang, Nguyen, Danesh, Blanchette, Rizzo, Liu, Lockhart, Ayala, Banttari, Pokorny. Soil Science - (55.5 hr) Project Leaders: Nater, Dowdy, Cooper Grigal. Users: Rachid, Mason, Stevens, Laird, Miller, Kohls. Other University Departments - (46.0 hr) Project Leaders: Banerjee, Pruitt, Users: Lu, Brenda. Outside Corporate Clients - (20.5 hr) Medtronic, Northrup King, Schwach, Osmonics, American Petrographics. Users: Coscio, Sagers, Schwach, Domsteen, Wolter, Lukkarila. Classes & Tours - (18.0 hr) Project Leaders: MacDonald, Munter, Lockhart, Kennedy. Instrument Calibrations & Maintenance - (74.0 hr). Ahlstrand, Zeyen.

            Impacts
            (N/A)

            Publications


              Progress 01/01/90 to 12/30/90

              Outputs
              Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, repairs and demonstrations - 914.0 hrs. AGRONOMY & PLANT GENETICS (8.0 hrs.) Project Leaders: Somers, Wyse. Users: Kaeppler, Carlson, Schreiber. ENTOMOLOGY. FISHERIES & WILDLIFE - (24.0 hrs.) Project Leaders: Kurtti, Holtzenthal, Prokrym. Users: Kurtti, Munderloh, Ahlstrand, Prokrym, Lazzari, Andow, Witherwpoon. FOOD SCIENCE & NUTRITION - (129.0 hrs.) Project Leaders: Zottola, Davis, Gordon, Asp, Morris. Users: Grider, Umbach, Teggatz, Rooney, Schwach, Overdahl, Van Til, Spurlock, Davis, Asp. FOREST RESOURCES - (3.0 hrs.) Project Leader: Johnson. User: Ritter. HORTICULTURE AND LANDSCAPE ARCHITECTURE - (108.0 hrs.) Project Leaders: Carter, Waters, Markhart, Smith. Users: Ericson, Barker, Harper, Smith. PLANT PATHOLOGY - (370.0 hrs.) Project Leaders: Zeyen, Banttari, Blanchette, Lockhart, MacDonald, Stewart, Bushnell, Pokorny, Anderson, Groth, Young. Users: Zeyen, Ahlstrand, Nguyen, Lockhart, Blanchette, Cease, Burnes, Danesh, Banttari, Stewart, Long, Bormann, Haight, Kalis, Bouhida, Kittesak, Daqun, Jenkins, Roberts, Abad. SOIL SCIENCE - (35.0 hrs.) Project Leaders: Bloom, Nater. Users: Nater, Rachid, Mason, Bouabid, Gourley. OUTSIDE & OTHER-UNIVERSITY USERS, (Not Experiment Station Supported) - (77.0 hrs.) SERVICE. DEVELOPMENT AND CALIBRATION - (145.0 hrs.) CLASSES. DEMONSTRATIONS. TOURS - (32.0 hrs.).

              Impacts
              (N/A)

              Publications


                Progress 01/01/89 to 12/30/89

                Outputs
                Total hours used, electron microscopes, X-ray microanalysis equipment; includes calibration, repairs and demonstrations - 1137 hr. AGRONOMY & PLANT GENETICS - (6.0 hr) Project Leader: Somers. User: Bregitzer. ENTOMOLOGY, FISHERIES & WILDLIFE - (107.5 hr) Project Leaders: Kurtti, Holtzenthal, Ragsdale. Users: Kurtti, Munderloh, Ahlstrand, Jenkins, Lazzari. FOOD SCIENCE & NUTRITION - (238.0 hr) Project Leaders: Zottola, Davis, Gordon, Schafer, Morris. Users: Grider, Umbach, Teggatz, Riesterer, Schwach, Overdahl, Van Til, Lambert, Davis, Snead. FOREST RESOURCES - (52.0 hr) Project Leaders: Johnson, Perry, SarKanen, Schmidt. Users: Ritter, Duta, Shelley, Lin. HORTICULTURE AND LANDSCAPE ARCHITECTURE - (44.0 hr) Project Leaders: Carter, Waters, Markhart. Users: Ericson, Barker, Miller. NORTH CENTRAL FOREST SERVICE - (2.5 hr) Project Leader: O'Brien. User: O'Brien. PLANT PATHOLOGY - (413.5 hr) Project Leaders: Zeyen, Banttari, Blanchette, Kennedy, Lockhart, MacDonald, Stewart, Bushnell, Meronuck Mirocha, Szabo, Percich, Kommedahl, Disease Clinic. Users: Ahlstrand, Jiang, Lockhart, Zeyen, Blanchette, Cease, Burnes, Boehm, Danesh, Banttari, Ocamb, Stewart, Johnson, Long, McCain, Lazarri, Bormann. SOIL SCIENCE - (53.0 hr) Project Leaders: Bloom, Nater, Cooper. Users: Nater, Zhang, Rachid, Miller, Gruenhagen. OUTSIDE & OTHER-UNIVERSITY, (Not Experiment Station Supported) - (46.5 hr). SERVICE, DEVELOPMENT AND CALIBRATION - (161.0 hr) CLASSES, DEMONSTRATIONS, TOURS - (13.0 hr).

                Impacts
                (N/A)

                Publications


                  Progress 01/01/88 to 12/30/88

                  Outputs
                  Total hours used, electron microscopes, x-ray micronanalysis equipment; includescalibration, repairs and demonstrations - 939.0 hr. AGRONOMY & PLANT GENETICS - (6.0 hr) Project Leaders: Somers, Gengenbach. Users: Bregitzer, Martinez. ENTOMOLOGY, FISHERIES & WILDLIFE - (12.0 hr) Project Leaders: Kurtti, Holtzenthal. Users: Kurtti, Munderloh, Ahlstrand, Monson, Strand, Lazzari. FOOD SCIENCE & NUTRITION- (151.0 hr). Project Leaders: Zottola, Davis, Gordon, Asp. Breene, Morris, Harlander, Reineccius. Users: Grider, Olander, Herald, Umbach, Teggatz, Asp, Baisier, Reddy, Knutson. HORTICULTURE AND LANDSCAPE ARCHITECTURE - (13.5 hr) Project Leaders: Carter, Stadelmann, Users: Kerr, Erickson, Chung. NORTH CENTRAL FOREST SERVICE - (2.5 hr) Project Leader: Robbins. Users: Robbins, O'Brien. PLANT PATHOLOGY (461.5 hr) Project Leaders: Zeyen, Banttari, Blanchette, Kennedy, Pfleger, Lockhart, MacDonald, Stewart, Bushnell, Sundaram, Ash, Meronuck, Mirocha. Users: Ahlstrand, Jiang, Khaless, Lockhart, Bouhida, Zeyen, Blanchette, Cease, Burnes, Abad, Curran, Boehm, Bosch, Danesh, Ferji, Enebak, Banttari, Sundaram. SOIL SCIENCE - (145.5 hr) Project Leaders: Bloom, Rosen, Rust, Nater. Users: Olson, Nater, Decker, Ahlstrand, Zhang, Rachid, Rosen. OUTSIDE & OTHER UNIVERSITY, (Not Experiment Station Supported) - (33.5 hr). SERVICE, DEVELOPMENT ND CALIBRATION - (93.5 hr). CLASSES, DEMONSTRATIONS, TOURS - (20 hr).

                  Impacts
                  (N/A)

                  Publications


                    Progress 01/01/87 to 12/30/87

                    Outputs
                    Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, repairs and demonstrations - 1535.5 hr. AGRONOMY & PLANT GENETICS - (38.5 hr) Project Leaders: Gengenbach, Somers, Vance. Users: Hanft, McCullough, Bregitzer, Eberlein. ENTOMOLOGY, FISHERIES & WILDLIFE - (16.5 hr) Project Leaders: Kukrtti, Holtzenthal, Ragsdale, Monson. FOOD SCIENCE & NUTRITION - (165 hr) Project Leaders: Zottola, Davis, Gordon, Asp, Breene; Users: Grider, Hood, Herald, Brand, Meronuck, Rost, Reddy, Schanen, Knutson. FOREST PRODUCTS - (30.5 hr) Project Leaders: Schmidt, Ritter. Users: Pope, Ritter. FOREST RESOURCES - (149.5 hr) Project Leaders: Perry, Sucoff. Users: Newsom, Ellis, Wilton, Bold. HORTICULTURE AND LANDSCAPE ARCHITECTURE - (162 hr) Project Leades: Marc, Markhart, Carter. Users: Marc, Castonguay, Kerr. PLANT PATHOLOGY - (643.5 hr) Project Leaders: Zeyen, Banttari, Blanchette, Kennedy, Pfleger, Lockhart, MacDonald, Stewart, Bushnell, Sundaram, Anderson, Larsen. Users: Ahlstrand, Otjen, Jiang, Khaless, Lockhart, Bouhida, Blanchette, Cease, Russo, Burnes, Abad, Koga, Millet, Roberts, Lorang. SOIL SCIENCE - (52 hr) Project Leaders: Bloom, Graham, Rosen, Olson, Molina, Nater. Users: Erich, Ahlstrand, Zhang, Meyer, Zachmann, Rosen, McDermott. OTHER-UNIVERSITY, not Experiment Station Supported (77 hr). SERVICE, DEVELOPMENT AND CALIBRATION - (179 hr). CLASSES, DEONSTRATTIONS, TOURS - (22 hr).

                    Impacts
                    (N/A)

                    Publications


                      Progress 01/01/86 to 12/30/86

                      Outputs
                      Total hours used, electron microscopes, X-ray microanalysis equipment; includes calibration, repairs and demonstration - 2012 hr. ANIMAL SCIENCE - (119.5 hr) Project Leader: Graham; User: Schmehl. DESIGN, HOUSING & APPAREL - (5.5 hr) Project Leader: Johnson; Users: Nelson, Schmehl. FOOD SCIENCE & NUTRITION - (351 hr) Project Leaders: Zottola, Davis, Gordon, Addis, McKay; Users: Grider, Hood, Herald, Pearce, LePage, Brand, Barone, Pramila, Fiertag, Meronuck. FOREST RESOURCES - (218 hr) Project Leader: Perry; Users: Newsom, Ellis. HORTICULTURE AND LANDSCAPE ARCHITECTURE - (66.5 hr) Project Leader: Marc; User: Marc. PLANT PATHOLOGY - (803 hr) Project Leaders: Zeyen, Banttari, Blanchette, Kennedy, Pfleger, Lockhart, MacDonald, Stewart, Bushnell, Kommedahl; Users: Ahlstrand, Atcham, Otjen, Alvarez, Ericsson, Curran, Jiang, Khaless, Lockhart, Bouhida, Blanchette, Cease, Lee, Sabet, Russo, Burnes, Abad, Koga. SOIL SCIENCE - (60 hr) Project Leaders: Bloom, Graham, Rosen; Users: Erich, Decker, Rosen, McDermott. ENTOMOLOGY, FISHERIES AND WILDLIFE - (29.5 hr) Project Leaders: Kurtti, Miller, Radcliffe; Users: Kurtti, Miller, Epstein, Lagnoui. OTHER-UNIVERSITY, not Experiment Station Supported (83 hr). SERVICE, DEVELOPMENT and CALIBRATION - (259 hr). CLASSES, DEMONSTRATIONS, TOURS - (17 hr).

                      Impacts
                      (N/A)

                      Publications


                        Progress 01/01/85 to 12/30/85

                        Outputs
                        Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, repairs and demonstrations 1732 hr. Agronomy & Plant Genetics-(39 hr) Project Leaders: Phillips, Gengenbach, Oelke; Users: Springer, Armstrong, Clay. Animal Science - (112 hr) Project leader: Graham; User: Schmehl. Design, Housing & Apparel - (28) Projeject Leader: Johnson; Users: Nelson, Schmehl. Foodscience & Nutrition - (278 hr) Project Leaders: Zottola, Thompson, Davis, Gordon, Swan; Users: Davis, Gordon, Grider, MacDonald, Gettys, hood, herald, Pearce, LePage, Mattea, Yen-Con. Forest Products - (45 hr) Project Leader: Neuman; User: Fereshtehkhou. Forest Resources - (2) Project Leader: Perry; User: Newsom. Horticulture and Landscape Architecture - (179) Project Leaders: Hackett, White, Ascher, Markhart, Wilkins; Users: Ruemmele, Markhart, Velguth, Grueber, Wallerstein, Jai Jai. Plant Pathology - (597 hr) Project Leaders: Zeyen, Banttari, Blanchette, Kennedp, Lockhart, MacDonald, Stewart, Bushnell; Users: Ahlstrand, Atcham, Otjen, Wang, Ericsson, Yuan, Curran, Jiang, Slakov, Cease, Carlson, Livingston, Jackson. Soil Science - (77 hr) Project Leaders: Rust, Bloom, Grahm, Rosen; Users: Gruenhagen, Badraoui, Decker, Rosen, McDermott. Other-University - not Experiment Station Supported (64 hr). Demonstrations, Service, Development and Calibration - (311 hr).

                        Impacts
                        (N/A)

                        Publications


                          Progress 01/01/84 to 12/30/84

                          Outputs
                          Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, repairs and demonstrations 1757 hr. AGRONOMY & PLANT GENETICS - (18 hr.) Project Leaders: Phillips, Gengenbach, Brun; Users: Kowles, McCullough, Betts. AGRICULTURAL ENGINEERING - (13.5 hr) Project Leader: Gustafson; user: Sheng. ANIMAL SCIENCE - (138 hr) Project Leader: Graham; Users: Schmehl. DESIGN, HOUSING & APPAREL - (68.5) Project Leader: Johnson; User: Nelson. FOOD SCIENCE & NUTRITION - (522.5 hr) Project Leaders: Zottola, Morris, Thompson, McKay, Davis, Gordon, savaiano, Swan; Users: Stone, Davis, Romero, Hanson, Mohamed, Halambeck, Grider, MacDonald. FOREST PRODUCTS - (117.5 hr) Project Leader; Neuman; User: Fereshtehkhou. HORTICULTURE AND LANDSCAPE ARCHITECTURE - (74.5 hr) Project Leaders: White, Ascher, Markhart; Users: Ruemmele, Markahrt, Velguth. PLANT PATHOLOGY - (478 hr) Project Leaders: Zeyen, Banttari, Blanchette, Kennedy, Lockhart, Percich, MacDonald, Kommedahl; users: Ahlstrand, Sandness, Atcham, Zuzek, Graham, Otjen, Wang, O'Brien, O'Laughlin, Bildusas, Jackson, Cordes, Ericsson, Yuan, Curran, Schickli. SOIL SCIENCE - (80.5 hr) Project Leaders: Rust, Bloom, Molina, Munter; Users: Gruenhagen, Crum, Badraoui, Inskeep. OTEHR-UNIVERSITY, not Experiment Station Supported (55 hr). DEMONSTRATIONS, SERVICE, DEVELOPMENT AND CALIBRATION (191 hr).

                          Impacts
                          (N/A)

                          Publications


                            Progress 01/01/83 to 12/30/83

                            Outputs
                            Total hours used, electron microscopes, x-ray microanalysis equipment; includes calibration, repairs and demonstrations (1753 hours). AGRONOMY AND PLANT GENETICS - (11 hours) Project Leaders: Vance, Phillips; Users: Stade. AGRICULTURAL ENGINEERING - (17 hours) Project Leaders: Thompson; Users: Norwig, Ruten, Yen-Con. ANIMAL SCIENCE - (48 hours) Project Leader: Graham; User: Schmehl, Jajima. ENTOMOLOGY, FISHERIES and WILDLIFE - (82 hours) Project Leader: Brooks-Wallace, Harein, Moon, Ragsdale; Users: Tsang, Mardan, Strand. FOOD SCIENCE and NUTRITION - (342 hours) Project Leaders: Zottola, Morris, Davis, Gordon, Busta, Savaiano, Swan; Users: Stone, Halambeck, Grider, Zylema, Wagner, MacDonald, Chen. FOREST PRODUCTS - (71 hours) Project Leaders: Neuman, Schmidt; User: Fereshtehkhou. HORTICULTURE AND LANDSCAPE ARCHITECTURE - (50 hours) Project Leaders: White, Hackett; Users: Ruemmele, Cordero. PLANT PATHOLOGY - (773 hours) Project Leaders: Zeyen, Banttari, Blanchette, Kennedy, Lockhart, Percich, MacDonald; Users: Woods, Ahlstrand, Sandness, Fei, Atcham, Zuzek, Graham, Objen, Wingfield, Wang, Dietrich, Cypher, Schickli. SOIL SCIENCE - (164 hours) Project Leaders: Rust, Bloom, Molina, Munter; Users: Poole, Greunhage, Inskeep. Other-University, not Experiment Station Supported - (27 hours). DEMONSTRATIONS, SERVICE, DEVELOPMENT AND CALIBRATION (168 hours).

                            Impacts
                            (N/A)

                            Publications


                              Progress 01/01/82 to 12/30/82

                              Outputs
                              Total hours used, electron microscopes and x-ray microanalysis equipment - 1,909hours, including calibration, demonstrations and repairs.

                              Impacts
                              (N/A)

                              Publications


                                Progress 01/01/80 to 12/30/80

                                Outputs
                                Total hour usage, electron microscopes - 2,283 h, usage by department listed below, plus 454 h calibration and maintenace. Agronomy and Plant Genetics - (331 h.) Project leaders; R. Phillips, C. Vance; Users, L. Johnson, M. Albertson. Entomology, Fisheries and Wildlife - (39 h), Project leaders; M. Brooks-Wallace, A. G. Richards, L. Cutkomp; Users, Tsang, Richards. Food Science and Nutrition - (426 h.), Project leaders; E. Davis, Labuza, McKay, Morris, Zottala; Users, Su-In, Cloke, Katz, Snook, Zoltai, Halambeck, Schwach. Forest Products - (78 h.), Project leaders, Neuman-Ovelle. Horticulture - (3 h.), Project leader, Alverez-Brakke. Home Economics/Textiles - (16 h.), Project Leader, R. Johnson-Carter. Plant Pathology - (603 h.), Project leaders; E. Banttari, R. Blanchette, F. Frosheiser, B. Kennedy, T. Kommedahl, S. Krupa, B. Lockhart, E. Stewart, R. Zeyen; Users, Suzwik, Franc, Lemmetty, Hijano, Widin, Windels, godzik, Betzold, Maybee, Palm, Lawrence. Soil Science - (333 h.), Project Leaders; Blake, Bloom, Rust; Users, Li, Gantzer, Gruenhagen, Okusemi.

                                Impacts
                                (N/A)

                                Publications


                                  Progress 01/01/79 to 12/30/79

                                  Outputs
                                  Total hour usage, electron microscopes - 1851 h. Usage by department listed below: Agronomy and plant genetics - Westra, Crockett, Crookson (Proj. leader); Johnson, Kennedy, Halvorsen, Vance (Proj. leader); Albertson, Phillips (project leader) - 357 h. Entomology, fisheries and wildlife - R. Tsang, Brooks - Wallace (Proj. leader); Richards (proj. leader) - 78 h. Food science and nutrition - Su-In, Hsieh, Davis and Gordon (proj. leaders), Katz, Saltmarch, Labuza (proj. leader); Zoltai, McKay (proj. leader) - 444 h. Forest products - Ovalle, Neuman (proj. leader) - 53.5 h. Horticulture - Toivio, Stushnoff (proj. leader) 148 h. Plant pathology - Palm, Stewart (proj. leader); Widin, Denny, Kennedy (proj. leader); Windels, Kommedahl (proj. leader); Coscio, Krupa (proj. leader); Mogen, Groth (proj. leader); Betzold, Lockhart (proj. leader); Banttari (proj. leader); Ahlstrand, Berger, Zeyen (proj. leader) - 713 h. Soil science - Gruenhagen, Rust (proj. leader); Gantzer, Blake (Proj. leader) -174 h.

                                  Impacts
                                  (N/A)

                                  Publications


                                    Progress 01/01/78 to 12/30/78

                                    Outputs
                                    Total hours usage, electron microscopes - 1434 h. Usage by department listed below. Agronomy and Plant Genetics (211 h) - Albertson, Phillips (proj. leader); Crockette, Crookston (proj. leader); Johnson, Vance (proj. leader); maize and soybean cell cultures, alfalfa anatomy, rhizobium interactions. Animal Science (13 h) - King, Shoffner (proj. leader); muscle dysfunctions. Entomology, Fisheries and Wildlife (55 h) - Cranshae, Radcliffe (proj. leader); Richards (proj. leader); Tsang, Brooks-Wallace (proj. leader); insect cuticle, insect microflora and parasites, plant structures resistant to insects. Food Science and Nutrition (282 h) - Hsieh, Davis (proj. leader); Saltaurch, Labuza (proj. leader); structural compositional changes in food due to processing procedures. Horticulture (90 h) - Palmer, Li (proj. leader); structure of cold hardy plant tissues. Plant Pathology (484 h) - Berger, Morgan, Zeyen (proj. leader); Pusposenjojo, Warner, Palm, Stewart (proj. leader); Nystrom, Krupa (proj. leader); Palmer, Betzold, Lockhart (proj. leader); Banttari (proj. leader); plant viruses, mycoplasma, fungal structure, cellular disease resistance mechanisms, corn, alfalfa, soybean, barley, oat, wheat and ornamental diseases. SoilScience (89 h) - Gruenhagen, Rust (proj. leader); Combs, Dowdy (proj. leader); sewage effluents, clay mineral aging processes.

                                    Impacts
                                    (N/A)

                                    Publications


                                      Progress 01/01/77 to 12/30/77

                                      Outputs
                                      Total hour usage, electron microscope - 1005 h. Usage by Department listed below. Agronomy and Plant Genetics - Albertsen, Hansen, Phillips (proj. leader); Johnson, Barnes (proj. leader); Umbeck, Gengenbach (proj. leader); Johnson, Vance (proj. leader) maize and soybean cell cultures, alfalfa anatomy, rhizobium interactions - 70 h. Entomology, Fisheries, and Wildlife - Smich, Richards (proj. leader); Peng, Tsang, Brooks-Wallace (proj. leader); Ultrastructure of insects, insect microflora and parasites - 376 h. Food Science and Nutrition - Larsen, Baldwin, McKay (proj. leader); Johnson, Gerald, Davis (proj. leader); Bacterial plasmids in milk starter cultures, food texture and cooking processes - 179 h. Horticulture - Palmer, Johnson, Li (proj. leader); cold hardiness of cell structures - 81 h. Plant Pathology - Winslow, Banttari (proj. leader); Hsi, Sherman, Berger, Mogan, Zeyen (proj. leader), Lockhart (project leader); Johnson, Frosheiser (proj. leader); Mizicko, Stienstra and Pfleger (proj. leaders); Hsi, Stewart (proj. leader); Bergdahl, Johnson, French (proj. leader), Johnson, Brambl (proj. leader). Plant viruses, virus vectors, mycoplasma; fungal structure; corn, alfalfa, oat, wheat, bean and ornamental disease - 289 h. Soils - Rust (proj. leader) clay morphology - 10 h.

                                      Impacts
                                      (N/A)

                                      Publications


                                        Progress 01/01/75 to 12/30/75

                                        Outputs
                                        Total hour usage, electron microscope - 1404. Usage by Department listed below.Agronomy and Plant Genetics - maize embryo structure - 50 hr. Entomology, Fisheries, and Wildlife - Ultrastructure of insects insect microflora and viruses of honeybee's. - 451 hr. Food Science and Nutrition - Baldwin, j. Elstelhiou (L. L. McKay project leader) Bacteriophages and bacterial plasmids related to milk starter cultures - 210 hr. Plant Pathology J. Mizicko, W. Winslow (E.E. Banttari project leader); D. Anderson, P. Sherman (D. W. French project leader); L. Johnson, B. Chevone, M. Schrieber, S. Hsi (R. Zeyen project leader) T. Knous (F. Frosheiser project leader). Structure and diagnosis of plant pathogenic fungi, viruses and bacteria - 693 hr.

                                        Impacts
                                        (N/A)

                                        Publications


                                          Progress 01/01/74 to 12/30/74

                                          Outputs
                                          Total hour usage, electron microscope - 1441. Department, personnel and equipment usage listed below. Entomology - S. Selheimer, B. Chevone (proj lead A. G. Richards); T. Wang, R. Tsang (proj lead M. Brooks); E. Mussen (proj lead B. Furgala). Ultrastructure of insects, insect microflora and viruses - 791 hr.Food Science and Nutrition - L. MacKay. Bacteriophages and bacterial plasmids. 106 hr. Plant Pathology - D. Anderson (proj lead D. French); S. Buse (proj lead E. Banttari); L. Johnson (proj lead R. Wilcoxson); R. Beer (proj lead R. Zeyen). Ultrastructure and diagnosis of plant pathogenic fungi, viruses and insect vectors - 484 hr. Demonstrations - 60 hr.

                                          Impacts
                                          (N/A)

                                          Publications


                                            Progress 01/01/73 to 12/30/73

                                            Outputs
                                            Total hour usage, electron microscope - 1360. Agronomy - J. Forester & J. Givens (adv. R. Philips); Maize meiocytes, maize and bacterial chromosomes - 30 hr. Entomology - K. Boo, B. Chevone (adv. A. G. Richards); T. Wang, (adv. M. Brooks); E. Mussen (adv. Furgala). Ultrastructure of insects and insect viruses407 hr. Food Science and Industries - L. MacKay & Tatini. Bacteriophages and toxins (bacterial). - 26 hr. Plant Pathology - D. Anderson (adv. D. French); S.Buse (E. Banttari; L. Johnson (R. Wilcoxson); T. Thompson, R. Beer (R. Zeyen). Ultrastructure of plant pathogenic fungi, bacteria, viruses and insect vectors -857 hr. Demonstrations - 40 hr.

                                            Impacts
                                            (N/A)

                                            Publications


                                              Progress 01/01/72 to 12/30/72

                                              Outputs
                                              Total hrs usage, electron microscope - 1381. Department, personel and equipmentusage listed below. Agronomy: J. Forester, J. Stout, & J. Givens (adv. R. Philips & R. Kleese); Maize meiocytes and maize & bacterial chromosomes. 88 hrs. Entomology: K. Boo, B. Chevone (adv G. Richards); T. Wang, T. Kurtti, (adv M. Brooks); E. Mussen (adv Furgala). Ultrastructure insects and insect viruses. 495 hrs. Food Science and Industries: L. Ogden, (adv C. Morr); L. Mackay. Ultrastructure milk particles and bacteriophage. 21 hrs. Soil Science: E. Landa (adv R. Gast). Soil morphology. 10 hrs. Plant Pathology: S. Buse (E. Banttari); D. Anderson (adv. D. French); B. Sherrer (adv M. Kernkamp); L. Johnson (R. Wilcoxson); T. Thompson, R. Beer (R. Zeyen). Ultrastructure plant pathogenic fungi, bacteria, viruses and insect vectors. 747 hrs. Demonstrations: 20 hrs.

                                              Impacts
                                              (N/A)

                                              Publications


                                                Progress 01/01/69 to 12/30/69

                                                Outputs
                                                The type of research, personnel and equipment usage is listed below: Antoine, Pierre, Department: Soil Science. Advisor: R. H. Rust. Clay identification. Approximate microscope time: 25 hours. Josephson, Ronald, Department: Food Science and Industries. Advisor: Charles V. Morr. Examination of casein micelles in milk systems. Approximate microscope time: 25 hours. Lepley, Charles, Department: Agronomy. Advisor: Dr. Moss. Anatomical comparisons of two kinds of chloroplasts from corn. Approximate microscope time: 20 hours. Sun, Chihning, Department: Soil Science. Advisor: R. S. Adams. Study effectsof atrazine and mineral deficiency upon chloroplasts. Approximate microscope time: 50 hours. Approximate ultramicrotome time: 50 hours. Stout, John T., Department: Agronomy. Advisor: R. L. Phillips. Examination of maize meiocytes. Approximate microscope time: 20 hours. Approximate ultramicrotome time: 35 hours. Turner, oMax, Department: Soil Science. Advisor: R. H. Rust. Effect of chromium on soybean root cells. Approximate microscope time: 30 hours. Approximate ultramicrotome time: 20 hours.

                                                Impacts
                                                (N/A)

                                                Publications