Recipient Organization
UNIV OF CALIFORNIA (VET-MED)
(N/A)
DAVIS,CA 95616
Performing Department
Pathology, Microbiology & Immunology
Non Technical Summary
At present, infection with a viral agent does not acurately predict disease outcome- inapparent disease, minor system based dysfunction, fulminant disease, death. Veterinarians and researchers need better tools to determine if a particular infection will result in inapparent disease or severe disease. Specific vral promoter sequences of retroviruses may provide this information.
Animal Health Component
20%
Research Effort Categories
Basic
80%
Applied
20%
Developmental
(N/A)
Goals / Objectives
The long term goal of this project is in alignment with the previous project (Mutations in the CAEV virus determine disease outcome) but focused on the viral promoter sequence and more general in scope (broader range of viruses). Viruses of interest include CAEV and MVV, ENTV, JSRV and BLV.From tissue speciments obtained from animals with naturally occuring disease, we intend to PCR amplify the viral promoter and sequence using classic molecular biology techniques. Sequences will be compared to one another for evidence of mutations. Specific viral promoter mutations will be assessed for association with specific disease outcomes.
Project Methods
RNA isolation from tissues (standard kits)reverse transcripion of RNA to cDNA (kits)PCR amplification of viral sequences (published sequences, GenBank, unpublished data)Sanger sequencing (local vendor)Data comparison using statistical software package