Performing Department
(N/A)
Non Technical Summary
The University of Kentucky (UK) is one of only eight universities in the United States that has colleges of Agriculture (CAFE), Arts and Sciences (CAS), Engineering, Medicine, and Pharmacy on a single campus, leading to unique interdisciplinary collaborations and offering more than 200-degree programs at the undergraduate and graduate levels. CAFE and CAS serve the land-grant mission of research, education, extension, and outreach, which are networked to the state's 120 counties through the Kentucky Cooperative Extension Services. To promote education while maintaining cutting-edge research, we have established an analytical core in CAFE that houses several gas- (GC) and liquid-chromatography (LC) units. This core provides important analytical services for a wide range of applications and has capabilities to detect and measure multiple metabolites. This facility also serves out-of-state researchers. Thetriple quadrupole (TQ) liquid chromatography (LC)-mass spectrometry (MS) instrument will be usedfor targeted-analysis of analytes that are present at low levels and cannot be detected using our existing instruments. The LC-TQ-MS will become part of the Center for Agricultural and Life Science Metabolomics (CALM) core and will be available to researchers from CAFE, CAS, Chemistry, Engineering and Medicine as well as researchers from other KY and out-of-state and overseas institutions. The instrument is also essential for undergraduate, and K-12 outreach engagements. Acquisition of LC-TQ-MS will provide access to critical, state-of-the-art, analytical facilities and ensure continued success of ongoing research.
Animal Health Component
15%
Research Effort Categories
Basic
80%
Applied
15%
Developmental
5%
Goals / Objectives
The triple quadrupole (TQ) liquid chromatography (LC)-mass spectrometry (MS) instrument will be used for targeted-analysis of analytes that are present at low levels and cannot be detected using our existing instruments. The LC-TQ-MS will become part of the Center for Agricultural and Life Science Metabolomics (CALM) core and will be available to researchers from CAFE, CAS, Chemistry, Engineering and Medicine as well as researchers from other KY and out-of-state and overseas institutions. The instrument is also essential for undergraduate, and K-12 outreach engagements. Acquisition of LC-TQ-MS will provide access to critical, state-of-the-art, analytical facilities and ensure continued success of ongoing research.
Project Methods
The LC-TQ-MS system will be listed on the CALM websiteand will be administered and operated by theCALMfacility. This facility is directed bythe PDand operatedby two full time staff.LC-TQ-MS system will be available for constant use, barring servicing and installation of replacement parts.We envision flexible schedules for all employees to accomplish the goals of our research.Accordingly, our approach to managing the instrument is to reserve it for calibration, cleaning, method development and validation, and other applications that require the presence of the operator during normal working hours (8 hours/day), with the instrument set up to run samples unattended for routine LC-MS measurements during evenings and weekends.