Source: KANSAS STATE UNIV submitted to NRP
FUELING WINTER CANOLA CULTIVAR AVAILABILITY TO MEET NEW DEMAND FOR OIL
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1032231
Grant No.
2024-67013-42345
Cumulative Award Amt.
$500,000.00
Proposal No.
2023-11148
Multistate No.
(N/A)
Project Start Date
Jun 1, 2024
Project End Date
May 31, 2027
Grant Year
2024
Program Code
[A1143]- Conventional Plant Breeding for Cultivar Development
Recipient Organization
KANSAS STATE UNIV
(N/A)
MANHATTAN,KS 66506
Performing Department
(N/A)
Non Technical Summary
The project "Fueling Winter Canola Cultivar Availability to Meet New Demand for Oil" will deliver superior winter canola cultivars to growers in the Great Plains and other states. These cultivars are urgently needed to meet growing U.S. demand for canola oil. This new demand is a result of the EPA's 2023 approval of the canola oil pathways for use in renewable diesel, jet fuel, naphtha, liquified petroleum gas, and heating oil. The U.S. Energy Information Administration projects domestic production capacity for renewable diesel to more than double by 2025, from 2.6 billion gallons per year to 5.9 billion gallons per year.Clean Fuels Alliance America estimates that canola oil imports from Canada for renewable diesel production will equal 5.8 billion pounds per year, or roughly 700 million gallons. Therefore, a significant opportunity exists to produce additional domestic canola oil feedstocks for renewable diesel production over the next several years. In the early 2000s, winter canola acres rapidly increased in the Great Plains because of improved cultivars, observed rotational benefits to wheat, growing regional crush capacity, and increased research and extension activities by public and private entities. Despite recent setbacks in the region, a new end marketing opportunity is taking shape. A former canola and sunflower facility was purchased and is being recommissioned to crush canola and soybean near Goodland, KS. The plant will begin processing grain in fall of 2024, with a capacity of 11 million bushels per year. To meet capacity, the plant could crush 500,000 acres of canola sourced throughout the region. Therefore, winter canola acres and production must increase to satisfy current and future Great Plains and U.S. canola seed, oil, and meal demand. The Great Plains offers opportunities for acreage growth as winter canola has been vitally important to improving small grain cropping system sustainability and profitability. Hence, this proposal addresses the growing need for new winter canola cultivars to fulfill increasing demand. This will be accomplished by 1) testing, advancing, releasing, and licensing new conventional and Roundup Ready open-pollinated cultivars in collaboration with interested parties, 2) generating additional data through increased testing to support cultivar release, 3) testing new female, male-sterile hybrid parent lines for general adaptability and increased efficiency and performance, and 4) delivering cultivar and hybrid performance results, characteristics, and benefits to stakeholders and clientele.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20118481081100%
Knowledge Area
201 - Plant Genome, Genetics, and Genetic Mechanisms;

Subject Of Investigation
1848 - Canola;

Field Of Science
1081 - Breeding;
Goals / Objectives
The goal of this project is to deliver publicly available, agronomically sound, and high performing winter canola cultivars to producers in the Great Plains and other states. Greater yields, herbicide resistance, and agronomic superiority are needed to meet the growing domestic demand for canola seed, oil, and meal to support food, feed, and fuel end uses. The rationale for this project is to generate new data through additional testing to quickly identify adapted cultivars for introduction into the winter canola market. New data over a greater number of locations will accelerate our ability to identify cultivars meriting release. Increasing the location number gives us the opportunity to encounter additional genotype by environment interactions, observe a wider look at the effect of environment on key traits, and identify cultivars that may be more stable across environmental conditions. The introduction of new, winter canola cultivars will 1) provide renewable biofuel feedstocks, 2) enhance environmental quality and the natural resource base of agriculture, 3) sustain the economic viability of farms, and 4) enhance the quality of life of farmers and society as a whole. The long-term enhancements and benefits include improved cropping systems diversity of wheat-based agriculture, persistent grower confidence in canola as an alternative crop, superior yield consistency of canola cultivars, and increased canola production to meet U.S. domestic demand for canola seed, oil, and meal. Novel products for the winter canola market will be developed by this project. A step change in cultivar and hybrid performance, as well as an available, regional market will be the catalysts that reignite a stagnant canola industry in the Great Plains. This project could help bridge the gap between an open-pollinated cultivar driven market and the conversion to a new era of hybrids. The following objectives align directly with the program area of focus and will guide the project in achieving the expected results and outcomes.Test, advance, release, and license new conventional and Roundup Ready® open-pollinated cultivars in collaboration with interested parties across the Great Plains and other states. Safeguard new technologies through Plant Variety Protection and licensing. Deposit germplasm within the National Plaint Germplasm System for future preservation and utilization.Support broader testing within the National Winter Canola Variety Trial. Provide expertise and financial support to current and new collaborators to enable high performance testing. Additional locations will provide data to support future cultivar release.Test new female, male-sterile hybrid parent lines in line x tester combinations for broad adaptability to the Great Plains. Calculate general combining ability and heterosis for important traits to determine which parental lines produce the best hybrid combinations and propose specific parent lines for increase and release.Directly deliver cultivar and hybrid performance results and characteristics, and promote the benefits of experimental and newly released cultivars to stakeholders and clientele. Methods of delivery may include, but are not limited to, field days, production meetings both in person and virtually, extension publications, agronomy updates, radio and television interviews, and social media. Cultivar manuscripts will be published in the Journal of Plant Registrations.
Project Methods
Objective 1Conduct advanced cultivar testing that is above normal levels to expedite identification and release of cultivars. Data to be gathered include fall stand, fall vigor, winter survival, 50% bloom date, maturity date, lodging, shattering, plot weight, moisture, test weight, grain yield, oil content, and protein content. Opportunistic notes such as freeze damage; heat damage; disease infestation including ratings for blackleg, white mold, and alternaria black spot; and insect damage will be taken.These trials will be carried out in 2024/25, 2025/26, and 2026/27.Three Great Plains Canola Variety Trials (GPCVT) will be grown in Kansas and New Mexico. Three Roundup Ready Variety Trials (RRVT) will be grown in Kansas and Oklahoma.The GPCVT and RRVT entries will consist of 34 experimental cultivars, two check cultivars, and three to four replications arranged in a randomized complete block design (RCBD).All RRVT will be treated with 16 to 22 fluid ounces of Roundup POWERMAX® herbicide in the fall or spring between the 2- and 6-leaf growth stages or before bolting. Percent stand loss will be taken 14 days post application.Data will be analyzed using SAS or other statistical software. Individual locations will be analyzed separately and may be pooled to evaluate genotype by environment interactions. Appropriate protocols and statistical methods will be followed.To confirm canola-quality oil and meal on cultivars nearing release, individual plant selections of KSR4767 will be analyzed for glucosinolates of the defatted meal. Canola-quality selections will be bulked together to produce breeder class seed. This seed will be used to grow a Foundation seed increase in 2024/25.A seed increase of KSR4839S will take place in the 2024 greenhouse cycle. Samples meeting quality standards will be bulked and planted as a breeder seed increase in 2024/25 and Foundation seed increase in 2025/26.A seed increase of KSR4854S will take place in the 2024 greenhouse cycle. Seed will be grown in 0.13 m2 flats that have been treated with chlorsulfuron + metsulfuron at a rate of 0.5 g active ingredient ha-1, providing a final screening of SU herbicide carryover tolerant plants. Tolerant plants will be grown to maturity. Samples meeting quality standards will be bulked and planted as a breeder seed increase in 2024/25 and Foundation seed increase in 2025/26. KSR4767 will be proposed for release, and KSR4839S and KSR4854S will be proposed for increase in 2024 or 2025. New data could identify additional cultivars for potential increase, including a high oil conventional cultivar, KS4737. We anticipate cultivar release proposals will be brought before the Plant Genetic Materials Release Committee (PGMRC) in summers 2024, 2025, 2026, and 2027.Kansas State University Innovation Partners (KSUIP) is the owner of all disclosed intellectual property (IP) and collects royalties on licensed materials. Plant Variety Protection (PVP) and licensing are used to protect all developed IP.KSUIP disclosure and commercialization processes follow the Research Terms and Conditions for NIFA-funded projects. All IP inventions funded through federal grants are disclosed on iEdison.Feasibility and Pitfalls for Objective 1 - Completing this objective is highly feasible because of the canola breeding program's history of testing, releasing, and licensing cultivars is a testament to the ability to deliver new cultivars to growers. Potential pitfalls include unfavorable weather at testing sites and unexpected results to the quality analyses.Objective 2The National Winter Canola Variety Trial (NWCVT) provides yield and morphological data for commercial and experimental cultivars developed by public and private entities. Results enable new cultivar deployment in areas where they can be profitably marketed.Broader testing in the NWCVT will be implemented through contracts and financial support.Sites in the Great Plains, Mid-South, Northern Plains, and Southeast will be targeted.All NWCVT experiments will be an RCBD with 3 or 4 replications. Data will be analyzed by site using the SAS Proc GLIMMIX procedure and means will be separated using the LSMeans lines option.Data may be pooled by region and analyzed across locations using common checks.Feasibility and Pitfalls for Objective 2 - The feasibility for completing this objective is high as end market opportunities are driving the need for new performance data. Potential pitfalls include an inability to identify potential collaborators and poor weather throughout the growing season.Objective 3Test new hybrid parent lines in line x tester combinations. These field studies will be carried out in 2024/25, 2025/26, and 2026/27.20 hybrids will be tested in yield trials in Kansas, New Mexico, and Oklahoma.Trials will consist of the 20 hybrids, two checks, and three replications arranged in an RCBD. High Check Heterosis (%) = [(Hybrid - High Check)/High Check] x 100 will be used to gauge performance.Heterosis for winter survival and vigor traits will be calculated as High Parent Heterosis (%) = [(Hybrid - High Parent)/High Parent] x 100 to identify female parents with the greatest winter survival.Parents showing the highest potential will be increased to produce breeder seed of A/B-line pairs.The goal will be to bring three A/B-line pairs up for release by the PGMRC in summer 2025.All released A/B-line pairs will be disclosed to KSUIP.Combining ability and heterosis analyses will be carried out near Manhattan, KS in 2025-2026 and 2026-2027.This study will consist of the 9 new A-line (BC4s) x two R-line testers (18 hybrids), the corresponding B-lines (male fertile maintainers of the A-lines), the R-line tester, and two commercial hybrid checks (30 total entries). Experimental design will be an RCBD with three replications.Heterosis for yield, winter survival, and oil content will be calculated as: High Parent Heterosis (%) = [(Hybrid - High Parent)/High Parent] x 100 and High Check Heterosis (%) = [(Hybrid - High Check)/High Check] x 100.General combining ability (GCA) of the female inbred lines will be measured by taking the mean of the A-line x R-line tester combinations minus the mean of all hybrids in the test.The A-lines possessing the greatest GCA will be tested for a second year in hybrid combinations. Simultaneously, hybrid seed will be produced for broader testing across more sites in subsequent years.Feasibility and Pitfalls for Objective 3 - Interest in using KSU germplasm in hybrid combinations by commercial seed companies is growing, so hybrid testing data will be of needed to make informed selections of sterile female parent lines. Potential pitfalls include poor hybrid performance, low heterosis, and less than desirable GCA values.Objective 4Directly deliver results, characteristics, and promote the benefits of released cultivars to stakeholders.Rapid information dissemination through social media, radio interviews, and popular press will reach the broadest spectrum of stakeholders.Utilize the K-State Department of Agronomy eUpdates and reports of progress to disseminate trial results.Professional society meetings are excellent ways to inform the scientific community on the advancements in winter canola across the USA.Participate in project director workshops hosted by NIFA.Outreach activities will be evaluated through feedback provided by attendees.Feasibility and Pitfalls for Objective 4 - Experienced personnel are in place thus the feasibility is high. The demand for data shared will be a positive indicator that the project is successful. Potential pitfalls include farmer apathy toward canola, thus participation at these activities may be less than desired.

Progress 06/01/24 to 05/31/25

Outputs
Target Audience:The target audience for this project is primarily new and experienced canola growers, as well as seed industry partners and licensees. Seed suppliers will be interested in the results generated from these studies. Growers from the Central Great Plains states of Kansas, Oklahoma, and Texas, and north into the Pacific Northwest, will have particular interest as new varieties are broughtto market. End processors will favor newer, more consistent varieties to support a growing industry. Changes/Problems:The loss of elite trials to biotic stresses (unpredictable insect infestation) was a challenge during the project period. Also, new NWCVT testing sites did not have productive seasons because of uncontrollable abiotic stresses. As a result, we were slowed in our abllity to generate new data to support future advancement and release of new varieties, and we likely missed an opportunity to propose a conventional variety for release in 2025. This signifies the importance of multi-site, multi-year testing to overcome environmental conditions that can greatly impact production agriculture across the US. What opportunities for training and professional development has the project provided?The PD participated in and presented on this research at Canola Week 2024 in Saskatoon, SK, Canada in December.Canola Week 2024 provides updates on the state of the canola industry in Canada, agronomic challenges and successes, and the latest canola innovations from across the globe. The focus of the PD's presentation was how winter canola is being developed as a major crop in the U.S. Great Plains. How have the results been disseminated to communities of interest?For results dissemination, please note the accomplishments under Goal 4 above. What do you plan to do during the next reporting period to accomplish the goals? Complete the final analysis of the 2025 variety trial results. Plan for a breeder seed increase of KS5010, a widely-adapted, high-yielding, and winter hardy conventional line. Plan for a breeder seed increase of KSR5015S, a very high yielding Roundup Ready variety. Complete quality analysis of individual plant selections of KS4662, KSR4852S, and KSR4926S from the 2025 greenhouse breeder seed increases. Conduct combining ability analysis of female, male-sterile parent lines tested in the 2025 K-State hybrid trials. Plan for and create hybrid seed of promising female, male-sterile parent lines identified through the combining ability analysis in the 2025-2026 winter greenhouse. Make advancement and testing decisions for the upcoming growing season. Present a proposal to release KSR4852S winter canola to the Plant Genetic Materials Release Committee. Pursue a potential licensee for KSR4854S winter canola. Produce a PVP application for KSR4854S winter canola. Increase KSR4852S from breeder seed to foundation seed stage in the field. Create the first hybrid parent line release proposal for the male steriles, KSM047A and KSM052A.

Impacts
What was accomplished under these goals? 1. For goal 1, one new winter canola variety, KSR4854S, was released by the Kansas Agricultural Experiment Station.KSR4854S is resistant to glyphosate herbicide (Roundup Ready) and it possesses tolerance to Group 2 (sulfonylurea) herbicide residual in the soil. Post emergence applications of glyphosate can be applied to KSR4854S to provide nonselective control of troublesome weeds in cereal-based cropping systems. Possessing sulfonylurea herbicide carryover tolerance allows those who grow KSR4854S to avoid the long plant-back restrictions that limit canola production following an application of sulfonylurea herbicides in winter wheat. KSR4854S is winter hardy and is adapted to Kansas, Oklahoma, and the Pacific Northwest.KSR4854S will be offered to potential licensees under an exclusive agreement. Other potential new varieties include KS4662, a conventional winter hardy variety, and KSR4852S, a Roundup Ready variety with high oil content. KSR4852S possesses an oil content that is approximately 1.5% percentage points higher than commercial checks. Two private industry partners have expressed interest in these experimental varieties. We anticipate proposing KSR4852S for variety release in summer 2025 and KS4662 in summer 2026. The canola breeding project expanded testing at two sites in Kansas in fall 2024. We conducted one new intermediate yield trial in Caldwell, KS and one new K-State hybrid trial in Norwich, KS. The expectation is that the additional data generated will support advancement decisions of our elite experimental lines and hybrid parent lines. Unfortunately, we lost these sites because of an excessive armyworm infestation, rendering the plots unusable. We are planning to continue a similar number of tests at these sites in the upcoming season. Also, an one new Roundup Ready yield trial was planted in Oklahoma and one new K-State hybrid trial was planted in New Mexico. Unfortunately, these trials were lost during the growing season to feral hogs and severe weather, respectively. These cooperators will continue to grow new trials of elite K-State breeding materials in the coming season. Breeder seed increases were carried out in the 2024-2025 winter greenhouse cycle. Individual plants were harvested and will be analyzed for final fatty acid profile and glucosinolate content. Samples passing quality standards will be bulked together to produce breeder seed for future variety increases. Breeder seed increases were grown out for KS4662, KS4737, KSR4852S, and KSR4926S. 2. For goal 2, the project was able to expand National Winter Canola Variety Trial testing into key geographies across the U.S. during the project period. Elite materials from the K-State breeding program are tested in the NWCVT each season, providing a comparison of performance to some of the top winter canola varieties and hybrids developed by industry. Hays, KS, a dryland site in west-central Kansas, was selected because its location covers a large, untested area and benefit region of the state. The Davenport, WA site will provide additional data to support winter canola acreage growth in the Pacific Northwest. A site at McGregor, TX was chosen to support testing in the southern reaches of the Great Plains, where interest in winter canola was once strong. The Griffin, GA site provides testing across a key geography in the Southeast, where production has historically been supported and could supply a new crush facility in the Mid-South with feedstock for renewable diesel production. Of these sites, only the Griffin site will be harvested in summer 2025. The Hays and Davenport sites were lost to poor soil moisture conditions, preventing emergence after planting. The McGregor site was lost to poor vernalization in the spring. A warmer-than-normal winter did not produce cold enough temperatures to properly vernalize the crop. As a result, this trial will be moved to a site further north and west in Texas for the coming season. As the 2025 harvest wrapped up, plans were being made to continue these trials in future seasons. Additional sites are being targeted as well. 3. For goal 3, a preliminary analysis of previous years' hybrid trials was carried out across Kansas. Results of the combining ability analysis provided insights into which parental lines to continue to focus attention. As a result, twelve female, male-sterile lines were crossed to two testers to produce hybrid seed in the 2024-2025 winter greenhouse cycle. This seed will be used for testing in 2025-2026. Parental lines KSM047A and KSM052A continue to produce hybrids that yield positive and significant combining abilty for yield and oil content. Breeder seed increases of these two lines are being planned for the next greenhouse cycle. 4. For goal 4, awareness of this project was relayed at production meetings, field days, and radio interviews during the reporting period. Variety testing results from previous years and the objectives for this research project were shared at the canola pre-plant meeting in Harper, KS on August 6, 2024. The work of the K-State breeding program to advance hybrid parent line development for winter canola was discussed in an invited presentation during Canola Week in Saskatoon, SK, Canada in December of 2024. The variety testing objectives of the winter canola breeding program and progress on the hybrid parent line development project were shared at a winter canola field day in Hutchinson, KS on May 14, 2025. The release of KSR4854S was discussed at the meetings in Kansas. Two radio interviews were given in July 2024 highlighting a look ahead to the next season, based on results and observations from the recently finished harvest.

Publications