Source: UNIVERSITY OF NEBRASKA submitted to
UNDERSTANDING HOST, MICROBIAL, ENVIRONMENTAL, AND MANAGEMENT FACTORS TOWARDS METHANE MITIGATION IN RUMINANTS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1031664
Grant No.
2024-68012-41583
Cumulative Award Amt.
$5,000,000.00
Proposal No.
2023-07033
Multistate No.
(N/A)
Project Start Date
Jan 1, 2024
Project End Date
Dec 31, 2028
Grant Year
2024
Program Code
[A9221]- Reducing Enteric Methane Emission from Ruminants
Project Director
Kononoff, P.
Recipient Organization
UNIVERSITY OF NEBRASKA
(N/A)
LINCOLN,NE 68583
Performing Department
(N/A)
Non Technical Summary
Ruminant-based agriculture is expected to continue to be a major contributor to feeding the increasing population. However, ruminant agriculture is also one of the major sources methane, a potentgreenhouseand contributer to climate change. Microbial methane productionin ruminants is influenced by many factors most notably animal genetics, diet, and the gut microbes. However, our understanding of microbial processes within ruminants across gene pools and in diet combinations that lead to methane production or inhibition is limited. Therefore, understanding factors and processes that underpin microbially mediated methane changes, and associated interactions is critical towards mitigating methane emissions. This proposal attempts to understand genetic, management, diet, and microbial relationships to develop science-based dietary and management intervention strategies to reduce greenhouse gas emission from cattle. Additionally, this application plans to develop novel microbiome strategies based on microbial colonization in the gut of cattle. The project will deliver research findings and management strategies to reduce methane production in dairy and beef cattle production systems to effect change in management through outreach. This work is expected to translate to the development of management and microbiome establishment strategies that reduce methane emission and establish an network that will improve the capacity nationwide to educate stakeholder concerns and provide tools and material that will help producers to assess and adopt practices that mitigate methane emission.
Animal Health Component
75%
Research Effort Categories
Basic
25%
Applied
75%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3023310108025%
3023410108025%
3023310101025%
3023410101025%
Goals / Objectives
We plan to quantify host, microbial, environmental and management factors that influence methane production in ruminants. Specifically, we will pursue the following objectives:1) Intensively phenotype dairy and beef cattle using indirect calorimetry for methane production, whole animal energy and nitrogen utilization while simultaneously collecting animal genomic data and phenotypic information of the microbiome through metagenome shotgun sequencing.2) Identify specific animal genetic, and microbial features that contribute towards or inhibit methane production to develop science-based intervention strategies for methane mitigation.3) Develop new microbial colonization methods based on colonization history and funder hypothesis of microbial establishment to develop microbiomes with capacity to decrease methane production.4) Develop science-based microbiome intervention strategies and applied practices and deliver educational opportunities to help stakeholders mitigate methane production in ruminants.
Project Methods
Efforts: Deliver science-based knowledge to learning communitoes through both formal or informal educational programs. Examples include: cooperatibe learning communities, workshops, demonstrations, development of programing;extension and outreach.Evaluation:Formative evaluation feedback will be conducted and presented to the program team on an annual basis and will inform the program by providing information needed to most effectively meet the proposed goals and objectives. The impact of the activities relative to the program's goal and objectives will be assessed in the summative evaluation. The formative and summative evaluations will rely on a combination of qualitative and quantitative data. Participants in the direct education activities will complete pre- and post-surveys to capture baseline and end-point opinions on methane mitigation, knowledge, issue awareness, and interest in change. The pre-surveys will include questions about prior experience, interests, and concerns going into the training that will be immediately communicated to grant leadership. The post-surveys will also include confidence in using what they learned, expected use, anticipated barriers and facilitators, interest in further education, and feedback on the session itself. The cooperative learning pods participants will also receive a mid-point survey to capture formative feedback about the process so far and any questions or concerns they have at that point. All direct education participants will also be asked to complete a follow-up survey including questions about their knowledge retention, use of what they learned (including any changes in management practices), suggested additional resources or education, and further plans to mitigate enteric methane. The videos of the cooperative learning pods will be reviewed and coded for changes in critical thinking skills and cooperative interactions over the four weeks. Cognitive interviews will be conducted with producers, Extension partners, and other expected audience members as part of the review of education materials produced as part of the grant. The goal of this is to assess the effectiveness of the format and messaging for the audience and purpose. Web analytics will be captured over time to assess the use of the on-demand access materials. Those accessing those videos and factsheets over the web will also see a survey link with questions such as how informative the information is, expected use of what they learned, overall feedback, and suggestions for future content. Finally, the Project Directors and other key project partners will be interviewed annually to capture successes and challenges for that year, changes made to planning, lessons learned, and overall progress towards grant goals.

Progress 01/01/24 to 12/31/24

Outputs
Target Audience:The target audience for this project are beef and dairy producers as well as scientists. Changes/Problems:The project has faced several challenges inclsuining rising costs of animals to be used in the project and feeds to feed them, identifcationof personnel to undertake experimental duties. At this point all of these challenges have been overcome but these will likely in a ongoing challenge. What opportunities for training and professional development has the project provided? The project hasofferedtraining and professional development opportunities through graduate and undergraduate mentoring, to a stratigic advisory group explaining the cooperative extenion learning pods. Project personnel have also presented mateials at internation meetings of scientists. How have the results been disseminated to communities of interest? Some earlyresults will be disseminated to communities of interest through various channels, including webinars, workshops, andsymposia. ? What do you plan to do during the next reporting period to accomplish the goals?Additionally, educational materials such as extension factsheets, videos, and online resources have been developed and will be made available on platforms like eBEEF.org and the UNL beef and dairy websites. ? The project team will also present findings at industry conferences and engage with producers directly through cooperative learning pods and on-site demonstrations.

Impacts
What was accomplished under these goals? The project aims to develop science-based intervention strategies to reduce methane emissions from ruminant agriculture by understanding the influence of host genetics, diet, environment, and management practices on microbial methanogenesis. ? In the last year we have 1) built 5 new headbox style indictrec calorimeters and calibrated 5 older units. These units have been used in data collectionon both dairy and beef animals. In addition to collectining phenotypes related to methane production and energy use we have collected animal tissue and rumen fluid toidentify genetic and microbial features that contribute to or inhibit methane production. We have also assmebled and met with a stratigicadvisorygroup to outline and discuss the projects objectives and audience.

Publications

  • Type: Peer Reviewed Journal Articles Status: Published Year Published: 2024 Citation: Tom, W.A., J.V. Judy, P.J. Kononoff, and S.C. Fernando. 2024. Influence of empirically derived filtering parameters, amplicon sequence variant, and operational taxonomic unit pipelines on assessing rumen microbial diversity. Journal of Dairy Science 107:92099234. doi:10.3168/jds.2023-24479.
  • Type: Peer Reviewed Journal Articles Status: Published Year Published: 2024 Citation: Carroll, A.L., M.L. Spangler, D.L. Morris, and P.J. Kononoff. 2024. Partitioning among-animal variance of energy utilization in lactating Jersey cows. Journal of Dairy Science 107:77347743. doi:10.3168/jds.2024-24740.
  • Type: Peer Reviewed Journal Articles Status: Under Review Year Published: 2024 Citation: Sherwood, S.C., A. L. Carroll, K. K. Buse, P. J. Kononoff. 2024. Methane production and whole animal energy utilization in lactating Jersey dairy cows fed an algae-based feed additive. Journal of Dairy Science. Submitted.