Source: WASHINGTON STATE UNIVERSITY submitted to NRP
ACQUISITION OF A VERSATILE PLANT PHENOTYPING PLATFORM (PHENOPLANT) TO ADVANCE FOOD AND AGRICULTURAL RESEARCH
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1031501
Grant No.
2023-70410-41210
Cumulative Award Amt.
$498,100.00
Proposal No.
2023-05466
Multistate No.
(N/A)
Project Start Date
Sep 15, 2023
Project End Date
Sep 14, 2027
Grant Year
2023
Program Code
[EGP]- Equipment Grants Program
Recipient Organization
WASHINGTON STATE UNIVERSITY
240 FRENCH ADMINISTRATION BLDG
PULLMAN,WA 99164-0001
Performing Department
(N/A)
Non Technical Summary
The new plant phenotyping instrument enables and stimulates fundamental and applied research, teaching, and collaborations with Washington State University, University of Idaho, USDA, extension centers, and regional scientist. The instrument, comprised of a multi-camera robotic imaging array and a water/nutrient control and measuring system allows non-invasive, automated monitoring of photosynthesis, fluorescent proteins, different shoot anatomical parameters, leaf temperature, hyperspectral data, transpiration rates, water-use-efficiency, stomatal conductance, growth rates, and biomass. The instrument will be localized in a state-of-the-art growth room. The unique measuring capabilities of the new instrument will stimulate research collaborations and advance the understanding of how crops will respond to future climate conditions. The new phenotyping facility will be advertised and offered to students, other scientists, teachers, stakeholders, and farmers. This will advance food production and precision agriculture in the Pacific Northwest and beyond.
Animal Health Component
30%
Research Effort Categories
Basic
60%
Applied
30%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2062499102050%
2032499102040%
4027210202010%
Goals / Objectives
The goal of this project is to establish a versatile and fully automated plant phenotyping platform enabling multiple fundamental and applied scientific programs to foster plant and agricultural research at Washington State University and the surround regions. It is aimed atstimulating research and teaching in plant biology, horticulture, plant and soil sciences, bioengineering and bioinformatics atWashington State Universityand other research institutions in the Pacific Northwest. The new facility will serve as a hub that will spark multidisciplinary research and teaching collaborations highly relevant for developing resilient and productive crop plants. In that way the new state-of-the-art plant phenotyping facility will close a critical gap in the Washington State University and the Pacific Northwest research infrastructure to integrate fundamental and applied plant and agricultural sciences.
Project Methods
The instrument consists of two computer-controlled parts: A optical imaging system and water managing and sensing system. The versatile imaging systems allows measurements of morphological and functional plant parameters ranging from photosynthesis, detection of fluorescent marker proteins (e.g., GFP), different shoot anatomical parameters, leaf temperature, and hyperspectral analysis. The water sensing system allows the non-invasive monitoring of whole plant water-use, nutrient availability, growth rates, transpiration rates, water-use-efficiency, and stomatal conductance under well controlled water and nutrient conditions. The precisely positioning of the imaging carrier above the plants employs the 'sensor to the plant' concept allowing non-invasive imaging multiple times per hours to once a day under well-defined illumination conditions. The control software automatically records the data with a time and position stamp. Measurements collected by the optical imaging system will be synchronized with the water managing and sensing system. Both systems will monitor plants throughout their growth allowing investigators to track changes in developmental stages and responses to stress that can be triggered at prescribed time points. This will lead to a full set of parameters defining plant performance. Processing of the data will be done by employing different bioinformatic methods.

Progress 09/15/23 to 09/14/24

Outputs
Target Audience:The newly established instrument will predominantly serve plant and agricultural scientists, alongside bioinformaticians from Washington State University (including extension centers) and the University of Idaho. Upon the establishment of the new facility and the demonstration of its analytical capabilities, scientists from various institutions across the Pacific Northwest will be encouraged to utilize the facility. Additionally, the new phenotyping platform will be employed under the guidance of scientists and instructors for the benefit of both graduate and undergraduate students. Moreover, schoolteachers, high school students, farmers, and stakeholders engaged in agriculture constitute the primary target audiences. Changes/Problems:Due to the necessity of installing a new robotic system and the requirement to remodel the growth room to accommodate additional electrical outlets for the new instrument, we were compelled to reorganize our budget. In order to render these changes cost-neutral, we were obliged to re-evaluate the PSI sensor system. The updated system retains full capability for the planned measurements, with the exception of hyperspectral imaging. The revised budget received approval from USDA-NIFA in December 2024. Furthermore, the necessity to provide access to the air conditioning system for maintenance mandated additional redesign of the instrument. These challenges resulted in a significant delay in the ordering process. What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?It is anticipated that the instrument will be fully installed and tested. We are currently in the process of hiring an instrument manager to ensure the instrument is operational and to conduct pilot experiments. The initial results will be utilized for an advertising platform disseminated to the collaborators of this USDA-NIFA project and other colleagues. The advertisement will encompass a detailed flyer for the facility, detailing measurement capabilities and potential outcomes. Furthermore, we plan to promote the new facility during seminars and lectures. PhenoPlant will be implemented within the Institute of Biological (IBC) Greenhouse Service Center, providing professional plant growth and technical support. A user fee structure will be established based on anticipated facility operating costs. Consequently, a budget will be developed under the guidance of the IBC financial manager.

Impacts
What was accomplished under these goals? The objective of this project is to establish a versatile and fully automated plant phenotyping platform (PhenoPlant), thereby enabling multiple fundamental and applied scientific programs to advance plant and agricultural research at Washington State University and its surrounding regions. Following the initiation of the project in September 2023, we contacted the two companies, Photon Systems Instruments (PSI) and PlantDitech, responsible for delivering the two components of the instrument, to finalize its design. It has been determined that an existing gantry system for transporting the optical sensors from PSI cannot be employed for the new instrument. Furthermore, the growth room designated for the new PhenoPlant systems requires certain electrical modifications. Both issues are elaborated upon in the 'Changes/Problems' section. These two challenges necessitated a redesign of the instrument, resulting in a delay in the purchasing process. Nevertheless, purchase orders were dispatched at the beginning of 2025, with deliveries anticipated in the summer or fall of 2025. Additionally, the necessary room modifications are currently underway. In collaboration with the companies mentioned above, detailed floor plans for the instruments have been finalized and approved. The floor plan considers access to an air conditioning system for regular maintenance by a technician. The electrical modifications required for the new instruments have been planned and coordinated with the Washington State University Facilities and Operations unit. The organization, instrument redesign, and coordination of tasks necessitated numerous meetings (exceeding 100 in total).

Publications