Source: FLORIDA ATLANTIC UNIVERSITY submitted to NRP
BUILDING CAPACITY IN MICROBIOME INNOVATION FOR PLANT HEALTH, SOIL FERTILITY AND ENVIRONMENTAL SUSTAINABILITY AT HISPANIC SERVING INSTITUTIONS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1031444
Grant No.
2023-77040-41197
Cumulative Award Amt.
$996,250.00
Proposal No.
2023-04494
Multistate No.
(N/A)
Project Start Date
Sep 15, 2023
Project End Date
Sep 14, 2027
Grant Year
2023
Program Code
[NJ]- Hispanic Serving Institutions Education Grants Program
Recipient Organization
FLORIDA ATLANTIC UNIVERSITY
777 GLADES RD
BOCA RATON,FL 33431
Performing Department
Department of Biological Sciences
Non Technical Summary
Reversing the projected shortage of skilled workforce in food and agriculture professions is both compelling and urgent to confront emerging challenges of climate change, feeding the burgeoning world population, declining soil health, and global trade dynamics.Intensive efforts to expand the recruitment of young people, especially Hispanic and under-represented populations have yielded significant dividends with increased enrollments in Hispanic-serving institutions such as Florida Atlantic University. However, such gains often confront serious retention challenges, draining the talent pipeline.The reasons for pipeline leakage are multifactorial, including a lack of requisite soft skills and socio-economic pressures. With the racial demographics of North America projected to reach 40% Hispanic by 2050, the importance of investing in quality education for this and other minority groups is irrefutable. Advancing the quality of education in these populations requires careful integration of diversity, equity, and inclusion principles with excellent curricula and effective mentoring.To recruit and graduate a competent and diverse workforce for the Food, Agriculture, and Natural Resource careers, FAU proposes an integratedprogram that will expand awareness of FANH careers to more than 4000 freshmen, develop a rigorous academic theme around microbiomes innovation, and promote critical thinking / soft-skill acquisition while providing access and scholarship for experiential learning and internships. This collaborative project with FIU will grow the recruitment of freshmen and improve graduation rates of Hispanic and underrepresented minority undergraduate and graduate students to 90%. Employing our unique model of multitiered mentoring by non-threatening role models, scholarship (~50% of budget), and immersion in structured academic research and internships with our partners, we will train and guide the students to graduate schools and jobs in FANH disciplines. In alignment with the priority goals of the HSI education program, we will enhance the quality of instruction by creating the FAU-Microbiome Innovation Center (MIC), a unifying theme for engaging students in multidisciplinary and cutting-edge research. A new course - Plant Microbiomes - will be developed and taught. Curriculum enhancement includes revision of plant biotechnology, microbial ecology, and FIU's soil microbiology. A benchtop DNA sequencer will augmentthe learning experience of students as they isolate, sequence, and curate a culture collection of indigenous beneficial bacteria for soil and plant probiotics and ensures project continuity beyond the grant. The outcome leverages internal support and grant monies to cost-effectively create enhanced curricula and pipeline at least 10 graduateand 15 undergraduates (total = 25) to advanced studies and the workforce.
Animal Health Component
50%
Research Effort Categories
Basic
20%
Applied
50%
Developmental
30%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1024010106040%
2155299110020%
1041629107020%
2032499104020%
Goals / Objectives
Recruit Hispanic, underrepresented minority students, and women through raising awareness about the many exciting career paths and opportunities in USDA and related agencies and sectors (ReachandRecruit@FAU-MIC). At least 1000 freshmen will be reached, and at least 15 undergraduates and 10 graduate students recruited and mentored.Improve retention through FAU microbiome innovation center's (MIC) "No Talent Left Behind" program - a robust stipend and mentoring support designed to attract the finest talents among this population and mentor them to graduation. About 90% of recruited scholars (entry post sophomore year) will graduate on time and be prepared for advanced studies or career workforce.Create a new Curriculum - Microbiome Innovation Center for plant health and soil sustainability where classic and emerging technologies for agriculture and environmental research are integrated. Also, we will develop one new course (Plant Microbiome) and revise curricula for plant biotechnology, microbial ecology, and soil microbiology and provide access totraining in genomic sequencing. We intend to purchase a benchtop sequencer for the first regional Microbiome Innovation Center (MIC) which will help enhance teaching and ensure continuity beyond the project lifespan.Forster comprehensive experiential learning opportunities for our students through internships and externships. We will leverage the collective expertise of the world-class Co-Principal investigators from our two large Florida Universities, USDA research centers, the American Farm Credit Council, Neighborhood Scientists Inc (a non-profit), and other partners to provide multiple laboratory research experiences that build core competence, analytical and problem-solving skills. The baseline research/technical and professional skills of this cohort of students will improve by at least 50% using pre and post-assessments.We will build consortium-wide leadership development programs - the "It takes a village@FAU-MIC" where leadership seminars and multi-level mentoring exchanges occur. We will sponsor graduate and undergraduate participants to national and regional conferences and mindset reset webinars to promote leadership skills and widen their network of collaborators and human resources.
Project Methods
Student Experiential Learning: We have designed an integrated experiential learning that leverages internally funded activities (Freshman and Sophomore Years) followed by two years of intensive MIC activities (Table 2). In addition to rigorous research every year of the program, scholars will participate in parallel mentoring, advising, internships, field trips, and professional development workshops and conferences. This program will immerse students in cutting-edge scientific research and provide them with soft skills, experience, and the support (career days) for pursuing an entry-mid-level career in FANH or further advanced degrees.Experiential learning activities designed for this project include the acquisition of novel advanced knowledge in genomics, microbial interactions, ecology, soil fertility, plant bioengineering, and sequencing technologies. All the activities will be focused on problem-solving, developing critical thinking skills, and preparing for careers in the FANH fields.?Academic year participation.Recruited and selected undergraduate students will be given a stipend to permit their participation in a yearlong research experience under the direction of any of the participating faculty. Student scholars become eligible for grant money at the end of their sophomore year. In all cases, students receive academic credit for their participation by enrolling in an Undergraduate Research Course of their choice; various options are available depending on the student's major department. Course credits can range from 1 to 3 credits per semester. At FAU, students write for grants and compete for internal funds disbursed by the OURI throughout the period they are registered with MIC. All students and participating faculty will participate in a mandatory day-long orientation where multiple mentors present careers in FANH and pathways to get there. Students will be taught the process of science, title/theme selection, hypothesis development and testing, literature review, organization, data gathering, data interpretation, field techniques, bio-safety, lab safety, and chemical hygiene practices. They will receive certificates issued by the Environmental, Health, and Safety Office (EHS). The skills gained will be expected to form the basis for the summer internship to follow. Students will be helped with finding a research faculty mentor of their interest. They are expected to complete a research project and present results at a university professional setting (such as the FAU OURI annual undergraduate research symposium), scientific presentation at a national conference or publication in a scientific journal. The graduate students at FAU will be selected and admitted directly into the MIC project. We will leverage FAU's internal funding for graduate Teaching Assistantships in their first year. This approach dispenses funds to only qualified candidates who have passed their thesis proposal exams and helps to stretch the reach of budgeted funds to cover more graduate students (#6) at the peak of their research and also makes room for costly research materials. FIU does not have similar support policies and so will enroll the grad students directly into the grant scholarship.Summer Research and Internships. All graduate and undergraduate students will be funded and required to engage in publishable research work with Faculty from both Universities (SURE - Summer Undergraduate Research Experience and GRES - Graduate Research Exposure in Summer). The collaboration will allow academic exchange between the faculty and staff, to the benefit of the students. At least two faculty, one from each of the collaborating institutions will provide input into a student's SURE or GRES project whenever possible.In addition, we will arrange for undergraduate students to have hands-on, career-focused 8-week summer internship experiences with USDA agency partners (USDA-NRCS, -APHIS, -ARS, -AMS or USFWS), academic research laboratories, and national research centersThe detailed curriculum of a new course (introduced by PD - Esiobu into the FAU catalog with scope approvals) - Plant Microbiomes MCB 6672 will be developed and offered to students at FAU & FIU during this project and beyond. Two courses in the University catalogs - Microbial Ecology and Plant Biotechnology are courses of high relevance and would markedly increase enrollment and attract young scholars in search of emerging technologies in 21st-centurycareers in FANH.These courses will be taught as Hybrid courses, combining in-person and virtual options, with the exception of laboratory components of the Plant Biotech course.Monitoring of student progress: All students will take Pre and Post program assessments through the online portal or in person. Two types of Pre and Post evaluations are administered viz Technical skill baseline assessment in fundamentals of techniques used in microbiomes (PCR, sequencing, qPCR), agriculture, lab safety, and other core research competencies. Cultural awareness/intelligence test which captures the baseline of ethics and professionalism for each candidate is also given. The post-assessments by the PI or project staff will test both technical and soft professional knowledge and career/professional development outcomes. Technical skills to be assessed include logic and critical thinking, the scientific research process, experimental design and methodologies, analyzing, interpreting, and communicating data, laboratory and field safety knowledge, and other specific skills like culture techniques, basic plant biology, tissue culture, etc. We expect that after a successful internship and experiential session, candidates will improve by up to 50% of baseline records.Mini-Pathways Fieldtrips, Workshops, Summer Camps, and More: Specialized training workshops and 'mini-pathways' field trips will provide a foundation for some of the experiential learning projects (ELP) programs of this grant. Students from all participating HSI institutions will come together annually to participate in a 7-day career-oriented field trip that incorporates training in USDA agency careers, and first-hand observation and training at the grower and industry levels.A culture collection of well-curated indigenous bacteria library will be developed. About 500 strains of beneficial organisms will be isolated from various phyllosphere, roots, etc., sequenced and characterized for use in synthetic microbiome applications. Bio-inoculants and biofertilizers made from characterized indigenous strains are an essential part of sustainable climate-smart agriculture. All the targeted FAU and FIU graduates and undergraduates will participate in isolating, sequencing, and curating their strains as part of the research experiential programs. The collection will become a valuable asset in customizing probiotics for specific plants in specific ecologies such as wetland grasses under salinity and flooding stress.Create a Microbiome Innovation Center (MIC). This will be the first center for microbiomes in South Florida. It will harness the different but highly complementary expertise of the Co-PIs from two large Universities to create a themed network of resources for the recruited students. Its physical location will be FAU where the PD - a black female professor already has some facilities and research ongoing in microbiomes of plants, environment, and animals (Dawkins and Esiobu, 2022; Bukar et al. 2019 and Dawkins and Esiobu, 2018).Leadership and professional development: Professional development opportunities for participating students, such as resume development, mock interviews, dressing for success, and effective communication will be provided at our annual career retreat (field trip). A centrally located field trip venue will be used to avoid the cost of a residential retreat.

Progress 09/15/23 to 09/14/24

Outputs
Target Audience:Outreach: Over 1,500 college freshmen in general biology and environmental studies were reached via in-class presentations, CANVAS announcements and social media, sensitizing them about career opportunities with the USDA-NIFA and the need for next-gen high tech professionals to solve food security challenges and emerging climatic challenges. Throughour grant sponsored and supported events, postings on campus, grant project website, e-mail communications via the college of science list of advisors, faculty, and students, at least 2000 additional individuals became intimated of the diversity of traditional and newer research and career opportunities in food and environment disciplines under the USDA-NIFA. With strong university support, we established the first Microbiome Innovation Center (MIC) in the region, as a rallying research theme for recruiting and training the next generation of professionals in technical and soft skills for the FANH workforce and graduate education. One undergraduate Hispanic scholar and one female minority scientist were engaged to work as OPS on the project. Two students (OPS on the grant) worked with PI to create flyers and recruitment/selection questionnaires and advertised the same online. Brochures for the FAU MIC program activities and events were developed and shared broadly, including at College of Science Festivals and Fairs where regional High schoolers and undergraduates shared the intrigue of science. Two career fairs and one seminar which uniquely featured academia (FAU - PI), industry (US Sugar) and government (USDA Canal Point & USDA south Florida Liaison) were organized, reaching more than 350 students - mostly sophomores, juniors and juniors curious about future directions and careers. Several FAU student-centered services and MIC advisory board members, our collaborative institution (FIU) was instrumental in providing resources and an opportunity for students to network and learn about careers in food, agriculture, and natural resources. Recruited: Twelve undergraduates (6 males and 6 females) of underrepresented minority groups were recruited in their sophomore and junior years as the first cohort of the capacity building grant. About 33% have maintained a cumulative perfect GPA of 4 while on the program. Two of the students are first generationcollege students. Advisory Board and Partnerships: A powerful and committed advisory board for the MIC program was set up to provide quarter feedback on the activities of the grant. It consists of 3 high level FAU faculty/staff, one renowned MIT alumn, one USDA Liaison to South East USA, a top executive of the US Farm Council and a collegerepresentation of the largest pipeline of minority STEM talents. One External Evaluator was identified and recruited to provide independent assessments and inputon data management Changes/Problems:Recruitment of eligible graduate students is highly competitive and has been slow. Many other programs on Campus offer more competitive stipend. We have however developed a system to integrate FAU funds and the grant stipend. The PI of the project Plans to retire at the end of Spring 2025. She however intends to remain closely associated with the grant until the end as a Professor Emeritusif the regulations permit.Nevertheless, one of the co-Pis has been identified to step up as lead PI (as needed) and is understudying the grant programs and leadership. The program directors have been informd of this development There are no major changes in the grant programs and goals. What opportunities for training and professional development has the project provided?Several professional development opportunities were provided as follows? (Skills / Opportunities) Public Speaking / Science Communication?: Opportunity and mentoring were provided for the MIC students to practice and presentat three scientific conferences and symposia, including at oral poster presentations at theFlorida American Society for Microbiology (FLASM, St Augustine, FALL 2023)?, ScienceFest and University-wide undergraduate research competition? Creativity, organisational and leadership skills:Training at the FAU center for new Campus clubs on how to develop a new club.. MIC students created their constitution for MANRRS, attended budgeting training sessions and conducted their own elections. Team workand productive team management:Productive work on teams is a necessary skill in life. Students are provided opportunities to work on some aspects of their projects as individuals and others are addressed as teams. Also, they were allowed to plan and executed the launch of the brandnew student club which had wide participation and impact, as a team!? Marketing Skills Imparted - Through program and activity planning and execution (eg USDA-MIC CAREER DAY, ScienceFest, Field trip and Conference planningetc)? Research Intergrity and data management: All the students provided the opportunity to take and pass courses on lab safety, fire safety, lab harzard management, chemical hygiene practices, blood borne pathogen and Citi research intergrity course before they resume handson activities on this grant.. These coursesconfer portable skills that will prove benefitial for the market place and in everyday living. Professional and scientific writing: FAU offers a required course for scientific writing for graduate students. Our first year cohort are mentored by their advisors and co-PIs. Students were allowed to write and apply for grants, outside internships and professional schools. All but one of the grant submissions was funded. Further the students maintain a culture of writing by updating their folders on the One drive which is monitored on an adhoc basis. ?? Critical thinking skills and analysis of data?: On a regular basis, the mentor meet with the students to guide them in the art of scientific reasoning, hypothesis articulation and critical dissection of literature Cutting edge technical skills and intense research training?- This is done at a regular pace during the academic Semesters and intensifies over the Summer Experiential learning. The OPS female scientist in the MIC lab and the PIs train the students during one on one meetings. This year, fourMIC students participated at Florida Atlantic Research Labs (PI) N=2, collaborators labs -. US Sugar USDA Canal Point N=1 and John Hopkins N=1) Four immersive experiential learning with USDA research station/US Sugar, Canal Point How have the results been disseminated to communities of interest?The most part of this grant year 1 was used toestablish all the compliance requirments, create an advisory board, procure a nanopore sequencer and set up the labs for intensive research experience. Forms and brochures for program activities and application flyers are posted online at our various handlers, including on our website and LinkediN https://biology.fau.edu/mic/index.phpStudents were screened and selected. Training these students has been the major focus. We are in the process of updating these outlets currently. Our Canvas portal is regularly used for disseminating information to the MIC students What do you plan to do during the next reporting period to accomplish the goals?!) Recruit more students, especially graduate (MS) students. 2) Present (Teach) the newly developed curricula in Plant Microbiomes 3) Expand the Physical collection of Plant Growth Promoting bacteria 4) Invest time to disseminate outcomes, including peer reviewed publications and fairs 5) Continue with immersive summer research experiences 6) Expand to new partnerships within FAUand consolidate current relationships to give student vast perspectives and learning opportunities 7)Conduct a field trip 8) Plan a collaborative activity for our FAU andthe FIU partner institution students.

Impacts
What was accomplished under these goals? 1. Recruit: At least 1500 freshmen were reached through class announcements and presentations. In all over 2000 students and members of the community learnt about our grant activities and career oppotunities (Seminar, Career Fair etc). Eleven (11) undergraduates from underrepresented minority groups have been recruited. The first batch of graduate students will join in the Spring of 2025 2) Retain: Ten out of the 11 students from year one are currently enrolled (>90% retention). Infact, the 11th candidate pivoted early into an FANH job after an amazing experiential learning. So in principle,that is a 100% retention! Thirty three (33%) of the MIC candidates have a perfect GPA of 4/4. The rest rank from 3.3-3.9! This is an excellent outcome and could be partially attributed to the stipend which keeps them focused. 3) New Curriculum to Enhance learning in the discipline: A new course on Plant Microbiomes has been developed. It's syllabus and study resources have been identified. It will tentatively be taught in the Summer/Fall 2025. Other courses - Updated Plant Biotech Labs and Lecture will be presented in the Spring of 2025 4) Experiential learning opportunities: During year 1, we had more opportunities than the students could fill. We leveraged collaborations to place students at John Hopkins, USDA research Center at Canal Point and FAU Biotech research facilities. The students report of impact shows it was totally a transformative experience. Indeed, one of them followed the lead to change original plans of med school to accepting a job in the USDA-NIFA career fields.

Publications

  • Type: Conference Papers and Presentations Status: Other Year Published: 2023 Citation: Edouard, J., Faradyan, S., & Esiobu, N. (2023). Effects of Defunding the Rhizobiomes? Root Microbiome community structure within managed and uncultivated Citrus groves. Florida American Society For Microbiology (FLASM), St. Augustine, FL.
  • Type: Conference Papers and Presentations Status: Other Year Published: 2024 Citation: Damas, S., Botey, M., & Esiobu, N. (2024). Are Tomato Microbiomes involved in the resistance of some varieties to the deadly Early Blight disease? Florida Atlantic University, OURI Symposium, April 2024
  • Type: Other Journal Articles Status: Accepted Year Published: 2024 Citation: Botey, M., Esiobu, N (2024) Microbiome innovation technologies for sustainable sugarcane production in South Florida: A review . Current Trends in Microbiology.?
  • Type: Conference Papers and Presentations Status: Other Year Published: 2024 Citation: Shant Faradyan, Sirisha Madem and Nwadiuto Esiobu (2024) An Optimization of the Transformation of Lactobacillus Acidophilus ATCC 314: A Step Towards Probiotic Vaccines. FAU Microbiome Innovation Cluster. Summer Undergraduate Research Fellows Symposium. FAU OURI, August, 2024
  • Type: Conference Papers and Presentations Status: Other Year Published: 2024 Citation: Shania Henry and Nwadiuto Esiobu (2024) SOIL HEALTH MATTERS: How Do Genetically Modified Glyphosate-Resistant Soybeans Affect Plant and Soil Microbiomes Over Time?? Presented at the FAU College of Science Festival, March 2024