Progress 09/01/23 to 08/31/24
Outputs Target Audience:The process of developing new potato cultivars involves a diverse range of target audiences, including potato seed growers, commercial growers, shippers, processors, research and extension personnel, the food distribution system, and consumers. Additionally, the program provides theoretical and practical training to the next generation of professionals, including undergraduate and graduate students, postdoctoral research associates, and youth groups. By engaging young individuals, the program inspires and educates the next generation of agricultural professionals, fostering an early interest in plant breeding and potato cultivation. Changes/Problems:USDA-NIFA funds (this project) provide critical support for the core of the SW (TX, CO, and CA) Potato Variety Development Program. The Texas A&M University Breeding Program has been relying on complementary funds (royalties returned to the program, miscellaneous grants, and in-kind grower support) for many years to sustain personnel and activities. In 2023, Texas A&M University suddenly, and without consultation with the potato breeding group, discontinued returning royalties (18% of the total royalties received, representing about ~$175,000 returned to the breeding program in 2022). The Texas A&M decision will seriously impact the Texas A&M Breeding Program. Plans to fill up the gap will be developed. We will have to request more funds from USDA-NIFA (this project) in subsequent years and be more proactive in obtaining funds, likely for basic research, since funding breeding programs is very difficult. What opportunities for training and professional development has the project provided? 1. Hands-on research experience for undergraduate research assistants, graduate students, research associates and postdocs Eight undergraduate students were involved in practical research such as tissue culture, the generation of segregating populations, screening resistances to the major diseases, and field demonstrations providing them with early exposure to advanced breeding techniques and research methodologies. Five graduate students, 15 research assistants/associates/technicians, and eight postdoctoral scholars gained hands-on experience in significant research activities, including hybridizations, evaluating disease resistance, participating in state and regional trials in the field, and conducting genetic studies on quality and composition. 2. Formal education in several academic courses and guest lectures HORT313 Plant Physiology (Vales): 86 students were taught fundamental and advanced concepts of plant physiology, helping them understand the mechanisms of plant growth and development. HORT325 Vegetable Production (Vales): A guest lecture was delivered to 20 students, covering key aspects of vegetable production and integrating practical breeding insights. PSS 6322 Advanced Plant Breeding (Koym): A specialized guest lecture was provided to 10 graduate students, focusing on advanced techniques and methodologies in plant breeding. HORT580A6 Plant Sciences (Chitwood-Brown): Applied Statistics and Design: 20 students received training in applied statistics and experimental design, essential for conducting robust plant breeding research. Guest Lecture for the 4H organization (Vales): A guest lecture on potato breeding was delivered to 18 high school students in the 4H organization, introducing them to the field of plant breeding and its importance in agriculture. 3. Technical skill development Image-based phenotyping: Training in the use of advanced imaging technologies for phenotyping enabled team members to acquire skills in high-throughput and precise measurement techniques. Climate resilience and sustainability studies: Team members were involved in studies aimed at improving the climate resilience and sustainability of crops, equipping them with knowledge of sustainable agricultural practices. 4. Dissemination of research through various academic channels and networking with peers Peer-reviewed publications: 6 peer-reviewed articles were published, contributing to the scientific community's knowledge and providing researchers with experience in collaborative works and scholarly writing. Conference presentations: 29 oral and poster presentations were made at national and international conferences, allowing researchers to share their findings and network with peers. 5. Professional development through state-level, regional, and national trials: Participation in these trials provided extensive exposure to evaluating plant varieties under different conditions, enhancing the ability to conduct large-scale and comprehensive trials. 6. Education and training of researchers and stakeholders through field demonstrations and reports The project organized field demonstrations and developed comprehensive documentation for educational and practical useaiding in the education and training of researchers and stakeholders. How have the results been disseminated to communities of interest?The results of the project have been widely disseminated to various communities of interest through multiple channels. These efforts have ensured that the results of the project reach a broad audience, including scientists, industry stakeholders, and the general public. 1. Field days: Field days directly disseminated results to growers, researchers, and industry professionals. Field days were held in Bakersfield, CA (40 attendees), Springlake, TX (32 attendees), and Monte Vista, CO (84 attendees), covering the latest potato breeding practices, new/advanced selection demonstrations, and insights into breeding programs and field trials. 2. Field day handbook: A detailed handbook was created and disseminated during field days, serving as a resource for attendees to reference the methodologies and results of the breeding program. The field day is available through program websites. 3. Breeding reports: In-depth breeding reports were compiled and distributed, documenting the progress and outcomes of the breeding trials, and aiding in the education and training of researchers and stakeholders. The breeding report is available through program websites. 4. Academic publications: Published 6 peer-reviewed journal articles, ensuring that the scientific community has access to the latest research findings: 5. SW regional group meeting: The meeting held on January 30, 2024, facilitated discussions on trial results and advanced selections, providing a platform for networking and knowledge exchange among researchers, growers, and industry professionals. 6. Conferences and symposia: Presented findings at various national and international conferences, including the Entomological Association of America Annual Meeting, the Potato Association of America Annual Meeting, the National Association of Plant Breeders Conference and others. Team members shared findings with a diverse audience fostering collaboration and knowledge exchange. 7. Newsletters and popular press: Information about field days and trial results was distributed via multiple popular press venues and newsletters, ensuring that findings reached a broad audience, including local and regional communities involved in potato production. What do you plan to do during the next reporting period to accomplish the goals?1. Develop russet, red, white, chip, and specialty potato cultivars for both the fresh and processing markets incorporating resistances to the major diseases present in the SW. Plan: We will continue our efforts to develop new potato varieties that are resilient to environmental stresses and resistant to major diseases present in the Southwest (SW). Building on previous successes, hybridization and selection will proceed at Colorado State University and Texas A&M University, generating genetic variation and integrating desirable genes. Approximately 200,000 seedlings will be grown for initial selection under field conditions in Colorado and Texas. The Texas program will use the upgraded Genovix system for breeding data management, while Colorado will continue with Medius Re, combining results for WR and SW regional trials. We will expand genomic selection using the StageWise package, genotype new introductions, released varieties, and advanced clones, and employ marker-assisted selection (MAS) for PVY resistance in over 100 clones. By the end of this project, we aim to develop high-quality potato varieties with enhanced yield, market quality, disease resistance, and heat tolerance. 2. Evaluate advanced selections under diverse environments for agronomic, quality, and postharvest traits critical to the potato industry. Plan: We will evaluate advanced potato selections under diverse environments in Colorado, Texas, and California to assess yield, quality, disease resistance, and other traits. Promising clones will enter national and regional potato variety trials. We will focus on health attributes using high-throughput metabolomics through the Periodic Table of Food Initiative and evaluate total antioxidant activity and resistant starch in potatoes. Postharvest evaluations will include assessing blackspot susceptibility, storage characteristics, and chip and French fry quality, using advanced techniques like HPLC for glycoalkaloid measurement and colorimetric methods for amylose and amylopectin ratios. Additionally, we will use the Smart Spud device to reduce bruising during harvest and warehouse operations. 3. Assess climate resilience and sustainability of cultivars and advanced selections. Plan: We will intensify our evaluation of advanced potato selections across diverse environments to address heat stress in Texas, combining new genotypic and phenotypic data with existing datasets to obtain genomic-estimated breeding values using StageWise. Ph.D. students Amaka Ifeduba and Ao Jiao will study the genetic, morphological, and physiological effects of heat tolerance and the impact on tuber dormancy length. We will also evaluate nutrient and water-use efficiency through nitrogen rate experiments and irrigation treatments, analyzing environmental stability using multi-environment trials to refine evaluation methods and improve predictive models. 4. Develop and implement high-throughput methods to screen germplasm for critical traits and incorporate these traits into the breeding programs. Plan: We will develop and implement high-throughput methods to screen potato germplasm for critical traits. Disease evaluations will involve establishing protocols for screening potato clones for responses to soft and dry rot, PVY, and powdery scab. Image-based phenotyping using drone multispectral images will assess potato variety vigor and plant cover at different growth stages. Additionally, we will use image-based methods to evaluate skin color and skin set and perform genome-wide association studies (GWAS) and quantitative trait loci (QTL) mapping for traits such as dormancy, heat-induced tuber defects, and disease resistance. 5. Facilitate the commercialization of cultivars released by the SW. Plan: We will conduct trials across Texas, Colorado, and California to determine optimal cultural practices for potato production, including fertility requirements, plant population density, and irrigation needs. We will introduce tubers from field selections into tissue culture to develop disease-tested micro-propagated seed stocks and protect new varieties under Plant Variety Protection (PVP). Expected releases include experimental clones such as Horizon Russet, Crystal Russet, Dune Russet, and Duncan Russet, aiming to enhance the sustainability and productivity of the SW potato industry.
Impacts What was accomplished under these goals?
1. Develop russet, red, white, chip, and specialty potato cultivars for both the fresh and processing markets incorporating resistances to the major diseases present in the SW. In 2023, the potato breeding programs in Colorado and Texas planted 148,000 seedling tubers from 173 parental clones, focusing on resistance to Potato virus Y (PVY), late blight, and nematodes. Collaborations with regional programs outside the Southwest enriched the genetic pool for breeding. A total of 1,005 potato clones were chosen for further evaluation and propagation. Several selections from the 2023 SW Regional Trial were advanced or continued in the 2024 Western Regional Trials. For example, the experimental clone CO15016-1RUsto is a long russet and has Rysto gene and it was advanced to Western Regional Trials. 2. Evaluate advanced selections under diverse environments for agronomic, quality, and postharvest traits critical to the potato industry. In 2023, state-level and national processing trials were conducted in Texas, Colorado, and California. These trials evaluated plant growth, tuber yield, size, quality, and disease/pest resistance. Advanced selections were evaluated across multiple US states and in New Brunswick, Canada. The Texas and Colorado programs conducted trials in various locations to develop superior varieties for diverse markets and conditions. Entries in the Western Regional Trials, such as AC10376-2012-1W/Y and CO13003-1RU, demonstrated promising agronomic and quality traits, leading to their further evaluation. 3. Assess climate resilience and sustainability of cultivars and advanced selections. Climate resilience and sustainability emphasized genetic studies. Genomic tools were used to understand the genetic basis of traits and implement genomic selection in tetraploid potato breeding. Total yield, marketable yield, external and internal tuber defects, specific gravity, starch, reducing sugars, minerals, amino acids, chipping and fry qualitywere affected when potatoes grew under high-temperature conditions. Heat-tolerant potato clones have been identified. The genetic basis of traits affected by heat stress is complex (quantitative), and genomic selection is recommended to select clones and advance progenies. 4. Develop and implement high-throughput methods to screen germplasm for critical traits and incorporate these traits into the breeding programs. High-throughput screening methods were developed and implemented to enhance breeding efficiency. Image-based phenotyping using drone multispectral images was conducted at Tulelake, CA, to describe vigor differences between potato varieties at different growth stages. This technology showed potential for early disease detection and assessing plant emergence and stand establishment. The Texas program also processed UAS images to extract features such as plant count and vegetation indices, providing valuable data for breeding decisions. 5. Facilitate the commercialization of cultivars released by the SW. Efforts to facilitate commercialization included obtaining Plant Breeders' Rights (PBR) for Vanguard Russet in Canada and preparing PBR applications for Horizon Russet and Crystal Russet. Field demonstrations highlighted newly released varieties and advanced selections. Events such as the Kern Potato Variety Field Day and various field days in Texas and Colorado showcased the program's achievements to stakeholders. Additionally, teaching activities and publications disseminated knowledge and research findings, further supporting the commercialization process.
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Niebaum, B., Gray, C., Holm, D.G., and Chitwood-Brown, J. 2023. Phenotyping red skin color variation and retention in storage of Colorado State University potato germplasm via reflectance and L*A*B* values. Potato Association of America Annual Meeting, Charlottetown, PEI, Canada, July 25, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Yilmaz, E., Chitwood-Brown, J., and Chikh-Ali, M. 2023. Evaluation of male sterility associated with PVY resistance genes. Potato Association of America Annual Meeting, Charlottetown, PEI, Canada, July 25, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Chitwood-Brown, J. 2023. Breeding for virus resistance at CSU. WERA89, Denver, CO, March 16, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Chitwood-Brown, J. 2023. Concept proposal presentation. Colorado Potato Administrative Committee, Area II, Monte Vista, CO, February 16, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Chitwood-Brown, J. 2023. CSU potato breeding and selection program. Vice President of Research, CSU, Fort Collins, CO, February 10, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Chitwood-Brown, J. 2023. CSU potato breeding and selection program. Southern Rocky Mountain Agriculture Conference, Monte Vista, CO, February 7, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Holm, D.G., Chitwood-Brown, J., Gray, C., and Niebaum, B. 2023. The Colorado potato breeding and selection program: 1979-2023. Colorado State University Agricultural Experiment Stations Conference, Denver, CO, February 1, 2023. Poster.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Pandey, J., S. Gautam, D.C. Scheuring, J.W. Koym, and M.I. Vales. 2023.Variation and genetic basis of mineral content in potato tubers and prospects for genomic selection. Front. Plant Sci., 14, 1301297.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Blauer, J.M., V. Sathuvalli, B.A. Charlton, S. Yilma, C.C. Shock, N. Baley, R. Qin, E. Feibert, R.G. Novy, J.L. Whitworth, M.J. Pavek, N.R. Knowles, L.O. Knowles, N. Fuller, J.C. Stark, R.R. Spear, M. Thornton, N. Olsen, S. Jayanty, D.A. Navarre, M. Feldman, and M.I. Vales. 2023. Rainier Russet: A dual use russet potato with long tuber dormancy, excellent process quality, and high early harvest packaging efficiency. Am. J. Potato Res., 101, 17-33.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Pandey J., D. Thompson, M. Joshi, D.C. Scheuring, J. Koym, V. Joshi, and M.I. Vales. 2023. Genetic architecture of tuber bound free amino acids in potato and effect of growing environment on the amino acid content. Sci. Rep., 13, 13940.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Gautam, S., R. Morey, N. Rau, D.C. Scheuring, D. Kurouski, and M.I. Vales. 2023. Raman spectroscopy detects chemical differences between potato tubers produced under normal and heat stress growing Conditions. Front. Plant Sci.,14, 1105603.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Pandey, P., D.C. Scheuring, J.W. Koym, J.B. Endelman, and M.I. Vales. 2023. Genomic selection and genome-wide association studies in tetraploid chipping potatoes. Plant Genome, 16, e20297.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Baley, N., V. Sathuvalli, B.A. Charlton, C.C. Shock, S. Yilma, R. Qin, E. Feibert, M.I. Vales, R.G. Novy, J.L. Whitworth, J.C. Stark, M.J. Pavek, N.R. Knowles, J.M. Blauer, T.L. Brandt, Yi Wang, M. Thornton, and N. Olsen. 2023. Echo Russet: A russet variety with a high yield of marketable tubers, high processing quality, and few tuber defects. Am. J. Potato Res., 100, 1526.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Ifeduba, A.M. and M.I. Vales. 2023. Developing climate smart potatoes to overcome the challenges of global warming. Entomological Association of America Annual Meeting, National Harbor, Maryland, Nov 5-8, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Toinga-Villafuerte, S.E., M.I. Vales, and K.S. Rathore. 2023. CRISPR/Cas9-mediated mutations in the granule-bound starch synthase gene to obtain a waxy version of the potato variety Yukon Gold. Potato Association of America 107th Annual Meeting, Prince Edward Island, Canada, July 23-27, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Jiao, A., S. Gautam, J. Pandey, D.C. Scheuring, J.W. Koym, M.I. Vales. 2023. Genome-Wide Association Studies and Genomic Prediction for Potato Tuber Dormancy. Potato Association of America 107th Annual Meeting, Prince Edward Island, Canada, July 23-27, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Ifeduba, A.M., S. Gautam, J. Pandey, S. Toinga-Villafuerte, D.C. Scheuring, J.W. Koym, and M.I. Vales. 2023. Mechanisms of heat tolerance in Vanguard Russet potatoes. Potato Association of America 107th Annual Meeting, Prince Edward Island, Canada, July 23-27, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Vales, M.I., S. Gautam, A.M. Ifeduba, A. Jiao, J. Pandey, S. Toinga-Villafuerte, D.C. Scheuring, and J.W. Koym. 2023. Development of heat-tolerant potato varieties by the Texas A&M Potato Breeding Program. Potato Association of America 107th Annual Meeting, Prince Edward Island, Canada, July 23-27, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Gautam, S., J. Pandey, D.C. Scheuring, J.W. Koym, and M.I. Vales. 2023. Investigating the genetic background of potato tuber defects caused by heat stress. Tools for Genomics-Assisted Breeding in Polyploids Workshop, San Diego, CA, January 12-13, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Toinga-Villafuerte, S.E., M.I. Vales, and K.S. Rathore. 2023. CRISPR/Cas9 targeted mutagenesis of the granule-bound starch synthase gene in potato (Solanum tuberosum L.) to obtain amylose-free starch in the tubers. Plant and Animal Genome Conference XXX, San Diego, CA, January 13-18, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Ifeduba, A.M., S. Gautam, J. Pandey, S. Toinga-Villafuerte, D.C. Scheuring, J.W. Koym, and M.I. Vales. 2023. Developing potato cultivars for future climate scenarios: Understanding the mechanisms of heat tolerance in Vanguard Russet potatoes. National Association of Plant Breeders Conference, Greenville, SC, July 16-20, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Ifeduba, A.M., S. Toinga-Villafuerte, D.C. Scheuring, and M.I. Vales. 2023. Global-warming ready potatoes: redefining earliness as a heat tolerance strategy. Texas A&M Plant Breeding Symposium, College Station, TX, February 16, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Jiao, A., S. Gautam, J. Pandey, D.C. Scheuring, J.W. Koym, M.I. Vales. 2023. Genome-wide association studies of potato tuber dormancy in response to cold storage. Plant and Animal Genome Conference XXX, San Diego, CA, January 13-18, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Jiao, A., S. Gautam, J. Pandey, D.C. Scheuring, J.W. Koym, M.I. Vales. 2023. Exploration of the genetic background of potato tuber dormancy in response to cold storage. Tools for Genomics-Assisted Breeding in Polyploids Workshop, San Diego, CA, January 12-13, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Pandey, J., S. Gautam, D.C. Scheuring, J.W. Koym, and M.I. Vales. 2023. Genomic regions associated with mineral content in potato tubers and identification of clones with high genomic-estimated breeding value. Plant and Animal Genome Conference XXX, San Diego, CA, January 13-18, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Pandey, J., S. Gautam, D. Scheuring, J. Koym, M.I. Vales. 2023. Genome-wide association studies of mineral content in potato tubers and identification of clones with high genomic-estimated breeding value. Tools for Genomics-Assisted Breeding in Polyploids Workshop, San Diego, CA, January 12-13, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Gautam, S., J. Pandey, D.C. Scheuring, J.W. Koym, and M.I. Vales. 2023. Uncovering genomic regions associated with potato tuber defects under heat stress and identification of heat-tolerant clones. Plant and Animal Genome Conference XXX, San Diego, CA. January 13-18, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Pandey, J., D.C. Scheuring, J.W. Koym, S. Gautam, A. Jiao, A.M. Ifeduba, and M.I. Vales. 2023. Vanguard Russet potato: Unveiling excellence in fresh market. 7th Annual Customer Appreciation, Monte Vista, CO, December 7, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Ifeduba, A. M., J. Pandey, and M.I. Vales. 2023. Heat stress and potatoes: An underlying survival mechanism unveiled. Horticultural Sciences Poster Symposium, College Station, TX, College Station, TX, November 30, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Jiao, A, S. Gautam, J. Pandey, D.C. Scheuring, J.W. Koym, and M.I. Vales. 2023. Phenotypic evaluation and genetic analysis of potato tuber dormancy. Horticultural Sciences Poster Symposium, College Station, TX, November 30, 2023. Poster.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Ifeduba, A.M., W. Rooney, and M.I. Vales. 2023. Global-warming ready potatoes: Understanding the mechanisms of heat tolerance in Texas A&M varieties. Plant Breeding Circle, College Station, TX, February 10, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Gray, C. 2023. 2023 Tuber Maturity Trial Update. Colorado Potato Administrative Committee, Area II, Monte Vista, CO, December 15, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Chitwood-Brown, J. 2023. 2023 Potato Breeding Activities at CSU. Northern Colorado Potato Growers Meeting, CPAC Area III. Greeley, CO, November 8, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Chitwood-Brown, J. 2023. The next chapter in breeding potatoes at CSU. Potato Association of America Annual Meeting, Charlottetown, PEI, Canada, July 26, 2023. Oral presentation.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Fatema, M., Nalam, V., Chikh-Ali, M., and Chitwood-Brown, J. 2023. Screening wild and commercial potato for resistance to PMTV and TRV Necrosis: Towards molecular mapping of resistance genes. Potato Association of America Annual Meeting, Charlottetown, PEI, Canada, July 25, 2023. Poster.
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