Source: WEST VIRGINIA UNIVERSITY submitted to
DRONE-ASSISTED PRECISION IPM TO MANAGE AND PREVENT REINVASION OF INVASIVE PLANTS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1031053
Grant No.
2023-70006-40594
Cumulative Award Amt.
$200,000.00
Proposal No.
2023-02952
Multistate No.
(N/A)
Project Start Date
Sep 1, 2023
Project End Date
Aug 31, 2026
Grant Year
2023
Program Code
[ARDP]- Applied Research and Development Program
Project Director
Park, Y.
Recipient Organization
WEST VIRGINIA UNIVERSITY
886 CHESTNUT RIDGE RD RM 202
MORGANTOWN,WV 26505-2742
Performing Department
(N/A)
Non Technical Summary
Agroecosystems in the U.S. face major threats from invasive plants causing economic and environmental damages that are often irreversible without accurate management of the threat in a timely manner. However, few sustainable options are currently available for stakeholders to manage invasive plants effectively because many plant invasions (1) are located in remote and hazardous areas, (2) have a patchy distribution, and (3) are composed of multiple nonnative plant species. To overcome the limitations, this 3-year project develops a new IPM strategy for invasive plants by incorporating chemical control with aerial releases of natural enemy insects and native plant seeds. The goal of this project is to develop IPM tools with drone-assisted precision control of invasive plants to minimize the impact and spread of invasive plants such as mile-a-minute weeds (MAM). Two objectives of this project are optimizing precision herbicide application with drones against MAM (objective 1) and developing drone-assisted IPM tools by incorporating natural enemies and native plants (objective 2). Two major outcomes from this project are (1) field protocols for precision herbicide spray with drones and (2) aerial systems and protocols for drone-assisted precision IPM tactics to manage and prevent reinvasion of MAM and other invasive plants.
Animal Health Component
80%
Research Effort Categories
Basic
0%
Applied
80%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2160699310070%
2160799310010%
2160699114020%
Goals / Objectives
The goal of this project is to develop IPM tools with drone-assisted precision control and management of mile a minute (MAM), an invasive weed, to help minimize its impact and spread. There are two objectives to support the goal of this project: optimizing precision herbicide application tactics with drones against MAM (objective 1)anddeveloping an IPM strategy that uses herbicide first followed by drone-assisted deployment of natural enemies and/or native seed dispersal (objective 2).
Project Methods
Objective 1.we will determine the optimal rate of glyphosate applied by drones as a post-emergence control strategy. Specifically, the coverage and efficacy of aerial spray of glyphosate using drones will be determined in two different ways: (1) glyphosate droplet coverage with water-sensitive papers and (2) estimating MAM mortality after herbicide application.The field experiments will be conducted at the MAM monitoring plots, and we will use a randomized complete block design with four different levels of glyphosate concentrations and four blocks to overcome heterogeneity in field conditions (e.g., MAM infestation levels and edaphic conditions) among replicated blocks. Aspray drone will be flown to apply glyphosate on the four blocks.We will calculate a relative MAM coverage compared to MAM coverage in the control plot to determine the efficacy of aerial spray of glyphosate by drones. Because MAM coverages could be different in the plots, we will use a Before-After-Control-Impact (BACI) design which is an effective method to evaluate the efficacy of glyphosate in this study. Before the glyphosate application using a spray drone, we will use a survey drone to take aerial images to detect and define the boundary of living MAM patches to obtain baseline MAM infestation data in each plot.Data will be analyzed by repeated-measured ANCOVA followed by mean comparisons among the four different glyphosate rates (i.e., Tukey's HSD test) to determine the effect of different rates of glyphosate on MAM.Objective 2. we will develop a new IPM strategy that incorporates natural enemies and native seeds after initial herbicide control. We will use the glyphosate rate determined for site-specific control of MAM in objective 1 as the initial treatment and this will represent our experimental control. Specifically, the efficacy of the aerial release of natural enemies and aerial broadcast of native seeds, separately and combined, after herbicide treatment will be determined over two growing seasons.A rotary-wing drone will be used for the aerial release of Rhinoncomimuslatipes(Coleoptera: Curculionidae) and seeds because they are better suited for situations that require precise hovering/positioning capabilities, such as on very steep slopes or in dense vegetation. Our experiment will be conducted based on a randomized block design with control (pre-determined herbicide application) and treatment (either R. latipes or native seeds or both). We will use a rate of 0.45 kg/0.4 ha for each of the four native species used in the two treatments with native seeds. The seeds will be collected in the fall at or adjacent to the study site one growing season before spreading the seed by drone. Thefall-flowering plants are common to old fields and early stages of succession and give way to more shade-tolerant native species over time. The seed will be mixed for each aerial flight run. Consistent with objective 1, we will use a BACIdesign to evaluate the efficacy of native plant seeds for preventing MAM reinfestation or new invasive plants. Before the glyphosate application, we will use a survey drone to take aerial images to define the boundary of MAM patches within each plot. We will estimate all herbaceous species cover and woody species cover for a pre-treatment account of species composition before native seed addition. In the growing season after native seed addition, these same plots will be evaluated for all herbaceous species and all woody species cover.After two weeks post-glyphosate application, we will disperse native seeds to the same delineated MAM patches in the blocks with native seed addition using the assigned rates. To obtain data on MAM reinfestation coverage in each plot, survey drones will be flown every two weeks.For the two treatment blocks receiving natural enemies (herbicide + R. latipes, herbicide + R. latipes + native seed), R. latipes will be released aerially at 100 insects per plot targeted at the previously delineated MAM patches. The bug pod will be used for the release of R. latipes using drones. Rhinoncomimus latipes will be released after MAM germinates from the seed bank and has at least four to five true leaves forming for most of the germinating plants. After the aerial release of R. latipes, their presence and abundance will be monitored every two weeks to confirm the establishment of R. latipes in the blocks with the natural enemy treatment and to confirm their absence in the herbicide only and herbicide + native seed treatments. To obtain data on MAM reinfestation to the plots, aerial surveys with drones will be conducted every two weeks. From the aerial images, we will calculate the areas of MAM reinfestation. A comparison of all treatments will be made with ANCOVA using repeated measures for the two growing seasons of data in order to evaluate the relative success of MAM removal/reduction and native plant establishment after an initial control with glyphosate application.

Progress 09/01/23 to 08/31/24

Outputs
Target Audience:Our project has successfully reached a diverse range of audiences through various outreach efforts and educational programs. We have targeted both the general public and specific scientific communities, ensuring that our work reaches those who can benefit most from our research and educational initiatives. General Public and K-12 Education We provided 20 outreach events with the WVU Insect Zoo, engaging over 300 citizens. These events aimed to educate and inspire the public, particularly focusing on K-12 students and families. Our outreach activities served to increase public understanding of insect diversity and their role in ecosystems, while also raising awareness about beneficial insects and invasive species. Scientific Community To engage with the scientific community and share our research findings, we delivered 6 research presentations to various scientific audiences. These presentations allowed us to disseminate our results and contribute to the broader scientific discourse in our field. Stakeholders and General Public We conducted 5 workshops or talks specifically designed for stakeholders and the general public. These events focused on practical applications of our research and its relevance to agriculture and ecosystem management.By targeting these diverse audiences, we ensured that our work reached both those who can directly apply our findings (such as farmers and land managers) and those who benefit from increased awareness and understanding of invasive pest management. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?One graduate student was trained in the use of drones and sensors for aerial surveys of invasive plants, and aerial release of natural enemy insects and seed balls for integrated pest management (IPM) of mile-a-minute weeds. How have the results been disseminated to communities of interest?The results of this study have been disseminated via academic conferences (twoEntomological Society of America conferences), the West Virginia University Library Exhibit, and the West Virginia University Farm Field Day.In addition, we educated the general public about the use of integrated pest management for invasive plants via the WVU Insect Zoo outreach. What do you plan to do during the next reporting period to accomplish the goals?For objective 1, the field experiments will be conducted at the research sites to determine the optimal concentration and amount of herbicide using drones.We will examine the spray droplets captured on the water-sensitive papers to measure droplet density, percent area coverage, and spray efficiency. In addition, we will calculate a relative mile-a-minute weed coverage compared to that in the control plot to determine the efficacy of aerial spray of herbicide by drones. Aerial surveys before and after aerial application of herbicide, aerial surveys with drones and sensors will be conducted to determine the effect of aerial herbicide applications on mile-a-minute weeds. For objective 2, we will design an experiment to determine the effect of an integrated pest management (IPM) approach to manage and prevent reinvasion of mile-a-minute weeds. The treatments will include control (no management), herbicide only, herbicide + natural enemy insect, herbicide + natural enemy insect+ native seed. All herbicides, natural enemy insects, and native seed balls will be applied or released aerially using drones. After aerial releases and applications of control measures, aerial surveys with drones will be conducted every two weeks until the end of the growing season.

Impacts
What was accomplished under these goals? For objective 1, we selected research sites in West Virginia and Pennsylvania. To lay out the research plots, we conducted aerial surveys with drones and sensors. The aerial images were stitched to generate a composite image which then was used to locate patches of mile-a-minute weeds in each site. For objective 2, we developed native seed balls which will be used to prevent reinvasion of mile-a-minute weeds after initial control with herbicide. The balls were made of clay and native seeds of Canada goldenrod(Solidago canadensis L.).As arate of 0.45 kg/0.4 ha or 0.65 lb/acre (about 650,000 seeds) is expected for aerial release using drones, we developed different sizes and ratios of clay content to develop and test the germination rate of goldenrod seeds. We found that the seed ball with a diameter of 1 cm containing 50-60 seeds successfully germinated after aerial release.

Publications