Progress 09/01/22 to 08/31/24

Outputs
Target Audience:The primary target audiences served during the reporting period include food animal industries (cervid, poultry, swine,and cattle), veterinary practitioners, and state and federal animal health regulatory officials. These include the largest cattlegenetics producer in the US and a large and growing poultry industry in Ohio. Rapid accessioning, testing, and messaging ofsamples collected from animals with clinical signs consistent with a foreign animal disease (FAD) will remain a priority. Thesemay include submissions from slaughter facilities, commercial layer and broiler facilities and veterinary practitioners. The ADDLcompletes same day testing for a number of high consequence agents including foot and mouth disease virus (FMDV),highly pathogenic avian influenza (HPAI) virus, porcine epidemic diarrhea virus (PEDV), and porcine respiratory andreproductive syndrome virus (PRRSV). The ADDL provides testing for several infectious agents that have clinical signsconsistent with FAD viruses such as Senecavirus A (SVA), vesicular stomatitis virus (VSV) and epizootic hemorrhagic diseasevirus (EHDV). Results for all testing are distributed to submitters and animal health regulatory officials through the ADDL USALIMS system via e-mail, e-fax and/or the web-based portal. The ADDL is currently approved to message for many diseases includingavian influenza,FMDV, avian paramyxovirus 1 (APMV-1), classical swine fever virus (CSFV), African swine fever virus (ASFV), pseudorabies(PRV) and swine influenza (IAV-S). Secondary target audiences reached during this reporting period include veterinariansand students from a variety of educational institutions, veterinary clinics and companies. Educational opportunities includelaboratory instruction, practicum experiences and experiential learning in necropsy, histopathology and several lab sections. Extension and outreach opportunities include an ADDL newsletter, quarterly stakeholder meetings, outreach to veterinary clinics, and invitations to participate in industry meetings and advisory boards. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?October 2022 - AAVLD Meeting June 2023 - ASF Preparedness Workshop August 2023 - NVSL QMS training (basic and advanced) How have the results been disseminated to communities of interest?Laboratory results are provided to clients electronically using USALIMS. Results are provided to NVSL using messaging to the USDA LMS database and Portal Access. ADDL plans to introduce an online client portal for submissions and reporting in 2024. ADDL utilizes an API web-based server through USALIMS to receive submissions and/or share results with clients and the Swine Disease Reporting System (SDRS). A quarterly laboratory newsletter is published on the ADDL website and delivered to subscribers via email to communicate important updates and information. The Ohio ADDL completes an annual self-assessment that is submitted to the NAHLN Program Office that summarizes accomplishments. What do you plan to do during the next reporting period to accomplish the goals?The ADDL completed the annual Agreement for Participation in the NAHLN and is committed to actively participating as a Level 1 laboratory within the NAHLN. We plan to break ground early in 2024 on a new laboratory that will be called the Ohio Veterinary Diagnostic Laboratory (OVDL) and it will replace the current ADDL. The OVDL should be operational in 2026. The ADDL will maintain capability and capacity to respond to animal disease emergencies like the ongoing HPAI incident. During the next reporting period, the Ohio ADDL will 1. Support IT personnel - maintain the laboratory information management system and provide training opportunities; 2. Support a quality system - maintain AAVLD and ISO17025 accreditations and attend quality management training; 3. Support laboratory and administrative personnel - travel to meetings related to activity for the NAHLN, assist other laboratories during emergencies, utilize the APHIS laboratory portal; 4. Purchase equipmentthat directly increases laboratory testing capacity & maintain equipment service agreements - replaceaging equipment to improve capability and efficiency; 5. Electronic messaging enhancement - maintain and enhance electronic messaging for disease reporting and adhere to HL7 requirements; 6. Support APHIS- and NAHLN-related activities in the establishment of technical aspects of emerging disease detection and reporting protocols.

Impacts
What was accomplished under these goals? As a NAHLN Level 1 laboratory, the Ohio ADDL supports the NAHLN by providing the capabilities and capacities to support early detection, rapid response, and appropriate recovery from high-consequence animal diseases. In addition to supporting the NAHLN mission, the Ohio ADDL offers testing for regulatory diseases and elective diagnostics for veterinarians in Ohio and beyond. In 2022, the Ohio ADDL completed 453,135 tests; as of 12/21/2023, the Ohio ADDL has completed 474,303 tests in 2023. ADDL utilizes USALIMS v.2022.002. NIFA NAHLN Infrastructure funds are used to support the annual contract with Acclaim, the vendor of USALIMS. ADDL participated in several NVSL and NAHLN proficiency tests. All tests were successfully completed. Our yearly subscription for Q-Pulse is supported using this NIFA NAHLN Infrastructure funding. Q-Pulse maintains lab quality system records like standard operating procedures and associated worksheets, external documents, the lab quality manual, training records, and equipment asset records. NIFA NAHLN Infrastructure supported travel for lab leadership to attend the annual AAVLD meeting. New barcode printers and scanners were purchased to support high volume testing, particularly during the Ohio HPAI incident. In 2022, the Ohio ADDL completed 5,029 avian influenza PCR tests; as of 12/21/2023, the laboratory has completed 9,296 avian influenza PCR tests. The majority of the 2023 avian influenza PCR tests have been completed in November and December as part of the Ohio HPAI incident that involves 5 infected premises and their associated control areas and surveillance zones. The NIFA NAHLN Infrastructure funds support service contracts for critical lab equipment including biosafety cabinets, the digester, pipettes, autoclaves, and real-time PCR instruments. The Ohio ADDL interacted several times with the new Unknown Morbidity and/or Mortality Event (UME) group regarding cases with an unknown, possibly infectious etiology.

Publications

Progress 09/01/23 to 08/31/24

Outputs
Target Audience:The primary target audiences served during the reporting period include food animal industries (cervid, poultry, swine, and cattle), veterinary practitioners, and state and federal animal health regulatory officials. These include the largest cattle genetics producer in the US and a large and growing poultry industry in Ohio. Rapid accessioning, testing, and messaging of samples collected from animals with clinical signs consistent with a foreign animal disease (FAD) will remain a priority. These may include submissions from slaughter facilities, commercial layer and broiler facilities and veterinary practitioners. The ADDL completes same day testing for a number of high consequence agents including foot and mouth disease virus (FMDV), highly pathogenic avian influenza (HPAI) virus, porcine epidemic diarrhea virus (PEDV), and porcine respiratory and reproductive syndrome virus (PRRSV). The ADDL provides testing for several infectious agents that have clinical signs consistent with FAD viruses such as Senecavirus A (SVA), vesicular stomatitis virus (VSV) and epizootic hemorrhagic disease virus (EHDV). Results for all testing are distributed to submitters and animal health regulatory officials through the ADDL USALIMS system via e-mail, e-fax and/or the web-based portal. The ADDL is currently approved to message for many diseases including avian influenza, FMDV, avian paramyxovirus 1 (APMV-1), classical swine fever virus (CSFV), African swine fever virus (ASFV), pseudorabies (PRV) and swine influenza (IAV-S). Secondary target audiences reached during this reporting period include veterinarians and students from a variety of educational institutions, veterinary clinics and companies. Educational opportunities include laboratory instruction, practicum experiences and experiential learning in necropsy, histopathology and several lab sections. Extension and outreach opportunities include an ADDL newsletter, quarterly stakeholder meetings, outreach to veterinary clinics, and invitations to participate in industry meetings and advisory boards. Changes/Problems:We submitted a no cost extension to accommodate equipment service contracts that we set up at the beginning of the state's fiscal year, July 01. What opportunities for training and professional development has the project provided?October 2022 - AAVLD Meeting June 2023 - ASF Preparedness Workshop August 2023 - NVSL QMS training (basic and advanced) How have the results been disseminated to communities of interest?Laboratory results are provided to clients electronically using USALIMS. Results are provided to NVSL using messaging to the USDA LMS database and Portal Access. ADDL plans to introduce an online client portal for submissions and reporting in 2024. ADDL utilizes an API web-based server through USALIMS to receive submissions and/or share results with clients and the Swine Disease Reporting System (SDRS). A quarterly laboratory newsletter is published on the ADDL website and delivered to subscribers via email to communicate important updates and information. The Ohio ADDL completes an annual self-assessment that is submitted to the NAHLN Program Office that summarizes accomplishments. What do you plan to do during the next reporting period to accomplish the goals?The ADDL completed the annual Agreement for Participation in the NAHLN and is committed to actively participating as a Level 1 laboratory within the NAHLN. We plan to break ground early in 2024 on a new laboratory that will be called the Ohio Veterinary Diagnostic Laboratory (OVDL) and it will replace the current ADDL. The OVDL should be operational in 2026. The ADDL will maintain capability and capacity to respond to animal disease emergencies like the ongoing HPAI incident. During the next reporting period, the Ohio ADDL will 1. Support IT personnel - maintain the laboratory information management system and provide training opportunities; 2. Support a quality system - maintain AAVLD and ISO17025 accreditations and attend quality management training; 3. Support laboratory and administrative personnel - travel to meetings related to activity for the NAHLN, assist other laboratories during emergencies, utilize the APHIS laboratory portal; 4. Purchase equipment that directly increases laboratory testing capacity & maintain equipment service agreements - replace aging equipment to improve capability and efficiency; 5. Electronic messaging enhancement - maintain and enhance electronic messaging for disease reporting and adhere to HL7 requirements; 6. Support APHIS- and NAHLN- related activities in the establishment of technical aspects of emerging disease detection and reporting protocols.

Impacts
What was accomplished under these goals? As a NAHLN Level 1 laboratory, the Ohio ADDL supports the NAHLN by providing the capabilities and capacities to support early detection, rapid response, and appropriate recovery from high-consequence animal diseases. In addition to supporting the NAHLN mission, the Ohio ADDL offers testing for regulatory diseases and elective diagnostics for veterinarians in Ohio and beyond. In 2022, the Ohio ADDL completed 453,135 tests; as of 12/21/2023, the Ohio ADDL has completed 474,303 tests in 2023. ADDL utilizes USALIMS v.2022.002. NIFA NAHLN Infrastructure funds are used to support the annual contract with Acclaim, the vendor of USALIMS. ADDL participated in several NVSL and NAHLN proficiency tests. All tests were successfully completed. Our yearly subscription for Q-Pulse is supported using this NIFA NAHLN Infrastructure funding. Q-Pulse maintains lab quality system records like standard operating procedures and associated worksheets, external documents, the lab quality manual, training records, and equipment asset records. NIFA NAHLN Infrastructure supported travel for lab leadership to attend the annual AAVLD meeting. New barcode printers and scanners were purchased to support high volume testing, particularly during the Ohio HPAI incident. In 2022, the Ohio ADDL completed 5,029 avian influenza PCR tests; as of 12/21/2023, the laboratory has completed 9,296 avian influenza PCR tests. The majority of the 2023 avian influenza PCR tests have been completed in November and December as part of the Ohio HPAI incident that involves 5 infected premises and their associated control areas and surveillance zones. The NIFA NAHLN Infrastructure funds support service contracts for critical lab equipment including biosafety cabinets, the digester, pipettes, autoclaves, and real-time PCR instruments. The Ohio ADDL interacted several times with the new Unknown Morbidity and/or Mortality Event (UME) group regarding cases with an unknown, possibly infectious etiology.

Publications

Progress 09/01/22 to 08/31/23

Outputs
Target Audience:The primary target audiences served during the reporting period include food animal industries (cervid, poultry, swine,and cattle), veterinary practitioners, and state and federal animal health regulatory officials. These include the largest cattlegenetics producer in the US and a large and growing poultry industry in Ohio. Rapid accessioning, testing, and messaging ofsamples collected from animals with clinical signs consistent with a foreign animal disease (FAD) will remain a priority. Thesemay include submissions from slaughter facilities, commercial layer and broiler facilities and veterinary practitioners. The ADDLcompletes same day testing for a number of high consequence agents including foot and mouth disease virus (FMDV),highly pathogenic avian influenza (HPAI) virus, porcine epidemic diarrhea virus (PEDV), and porcine respiratory andreproductive syndrome virus (PRRSV). The ADDL provides testing for several infectious agents that have clinical signsconsistent with FAD viruses such as Senecavirus A (SVA), vesicular stomatitis virus (VSV) and epizootic hemorrhagic diseasevirus (EHDV). Results for all testing are distributed to submitters and animal health regulatory officials through the ADDL USALIMS system via e-mail, e-fax and/or the web-based portal. The ADDL is currently approved to message for many diseases includingavian influenza,FMDV, avian paramyxovirus 1 (APMV-1), classical swine fever virus (CSFV), African swine fever virus (ASFV), pseudorabies(PRV) and swine influenza (IAV-S). Secondary target audiences reached during this reporting period include veterinariansand students from a variety of educational institutions, veterinary clinics and companies. Educational opportunities includelaboratory instruction, practicum experiences and experiential learning in necropsy, histopathology and several lab sections. Extension and outreach opportunities include an ADDL newsletter, quarterly stakeholder meetings, outreach to veterinary clinics, and invitations to participate in industry meetings and advisory boards. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?October 2022 - AAVLD Meeting June 2023 - ASF Preparedness Workshop August 2023 - NVSL QMS training (basic and advanced) How have the results been disseminated to communities of interest?Laboratory results are provided to clients electronically using USALIMS. Results are provided to NVSL using messaging to the USDA LMS database and Portal Access. ADDL plans to introduce an online client portal for submissions and reporting in 2024. ADDL utilizes an API web-based server through USALIMS to receive submissions and/or share results with clients and the Swine Disease Reporting System (SDRS). A quarterly laboratory newsletter is published on the ADDL website and delivered to subscribers via email to communicate important updates and information. The Ohio ADDL completes an annual self-assessment that is submitted to the NAHLN Program Office that summarizes accomplishments. What do you plan to do during the next reporting period to accomplish the goals?The ADDL completed the annual Agreement for Participation in the NAHLN and is committed to actively participating as a Level 1 laboratory within the NAHLN. We plan to break ground early in 2024 on a new laboratory that will be called the Ohio Veterinary Diagnostic Laboratory (OVDL) and it will replace the current ADDL. The OVDL should be operational in 2026. The ADDL will maintain capability and capacity to respond to animal disease emergencies like the ongoing HPAI incident. During the next reporting period, the Ohio ADDL will 1. Support IT personnel - maintain the laboratory information management system and provide training opportunities; 2. Support a quality system - maintain AAVLD and ISO17025 accreditations and attend quality management training; 3. Support laboratory and administrative personnel - travel to meetings related to activity for the NAHLN, assist other laboratories during emergencies, utilize the APHIS laboratory portal; 4. Purchase equipmentthat directly increases laboratory testing capacity & maintain equipment service agreements - replaceaging equipment to improve capability and efficiency; 5. Electronic messaging enhancement - maintain and enhance electronic messaging for disease reporting and adhere to HL7 requirements; 6. Support APHIS- and NAHLN-related activities in the establishment of technical aspects of emerging disease detection and reporting protocols.

Impacts
What was accomplished under these goals? As a NAHLN Level 1 laboratory, the Ohio ADDL supports the NAHLN by providing the capabilities and capacities to support early detection, rapid response, and appropriate recovery from high-consequence animal diseases. In addition to supporting the NAHLN mission, the Ohio ADDL offers testing for regulatory diseases and elective diagnostics for veterinarians in Ohio and beyond. In 2022, the Ohio ADDL completed 453,135 tests; as of 12/21/2023, the Ohio ADDL has completed 474,303 tests in 2023. ADDL utilizes USALIMS v.2022.002. NIFA NAHLN Infrastructure funds are used to support the annual contract with Acclaim, the vendor of USALIMS. ADDL participated in several NVSL and NAHLN proficiency tests. All tests were successfully completed. Our yearly subscription for Q-Pulse is supported using this NIFA NAHLN Infrastructure funding. Q-Pulse maintains lab quality system records like standard operating procedures and associated worksheets, external documents, the lab quality manual, training records, and equipment asset records. NIFA NAHLN Infrastructure supported travel for lab leadership to attend the annual AAVLD meeting. New barcode printers and scanners were purchased to support high volume testing, particularly during the Ohio HPAI incident. In 2022, the Ohio ADDL completed 5,029 avian influenza PCR tests; as of 12/21/2023, the laboratory has completed 9,296 avian influenza PCR tests. The majority of the 2023 avian influenza PCR tests have been completed in November and December as part of the Ohio HPAI incident that involves 5 infected premises and their associated control areas and surveillance zones. The NIFA NAHLN Infrastructure funds support service contracts for critical lab equipment including biosafety cabinets, the digester, pipettes, autoclaves, and real-time PCR instruments. The Ohio ADDL interacted several times with the new Unknown Morbidity and/or Mortality Event (UME) group regarding cases with an unknown, possibly infectious etiology.

Publications

Progress 09/01/22 to 08/31/23

Outputs
Target Audience:The primary target audiences served during the reporting period include food animal industries (cervid, poultry, swine, and cattle), veterinary practitioners, and state and federal animal health regulatory officials. These include the largest cattle genetics producer in the US and a large and growing poultry industry in Ohio. Rapid accessioning, testing, and messaging of samples collected from animals with clinical signs consistent with a foreign animal disease (FAD) will remain a priority. These may include submissions from slaughter facilities, commercial layer and broiler facilities and veterinary practitioners. The ADDL completes same day testing for a number of high consequence agents including foot and mouth disease virus (FMDV), highly pathogenic avian influenza (HPAI) virus, porcine epidemic diarrhea virus (PEDV), and porcine respiratory and reproductive syndrome virus (PRRSV). The ADDL provides testing for several infectious agents that have clinical signs consistent with FAD viruses such as Senecavirus A (SVA), vesicular stomatitis virus (VSV) and epizootic hemorrhagic disease virus (EHDV). Results for all testing are distributed to submitters and animal health regulatory officials through the ADDL USALIMS system via e-mail, e-fax and/or the web-based portal. The ADDL is currently approved to message for many diseases including avian influenza, FMDV, avian paramyxovirus 1 (APMV-1), classical swine fever virus (CSFV), African swine fever virus (ASFV), pseudorabies (PRV) and swine influenza (IAV-S). Secondary target audiences reached during this reporting period include veterinarians and students from a variety of educational institutions, veterinary clinics and companies. Educational opportunities include laboratory instruction, practicum experiences and experiential learning in necropsy, histopathology and several lab sections. Extension and outreach opportunities include an ADDL newsletter, quarterly stakeholder meetings, outreach to veterinary clinics, and invitations to participate in industry meetings and advisory boards. Changes/Problems:We submitted a no cost extension request. The state fiscal year is 07/01 to 06/30 so we requested the extension to implement equipment service contracts in SFY2024 that we were waiting for approval on from the Legal Division at the Ohio Department of Agriculture. What opportunities for training and professional development has the project provided?October 2022 - AAVLD Meeting June 2023 - ASF Preparedness Workshop August 2023 - NVSL QMS training (basic and advanced) How have the results been disseminated to communities of interest?Laboratory results are provided to clients electronically using USALIMS. Results are provided to NVSL using messaging to the USDA LMS database and Portal Access. ADDL plans to introduce an online client portal for submissions and reporting in 2024. ADDL utilizes an API web-based server through USALIMS to receive submissions and/or share results with clients and the Swine Disease Reporting System (SDRS). A quarterly laboratory newsletter is published on the ADDL website and delivered to subscribers via email to communicate important updates and information. The Ohio ADDL completes an annual self-assessment that is submitted to the NAHLN Program Office that summarizes accomplishments. What do you plan to do during the next reporting period to accomplish the goals?The ADDL completed the annual Agreement for Participation in the NAHLN and is committed to actively participating as a Level 1 laboratory within the NAHLN. We plan to break ground early in 2024 on a new laboratory that will be called the Ohio Veterinary Diagnostic Laboratory (OVDL) and it will replace the current ADDL. The OVDL should be operational in 2026. The ADDL will maintain capability and capacity to respond to animal disease emergencies like the ongoing HPAI incident. During the next reporting period, the Ohio ADDL will 1. Support IT personnel - maintain the laboratory information management system and provide training opportunities; 2. Support a quality system - maintain AAVLD and ISO17025 accreditations and attend quality management training; 3. Support laboratory and administrative personnel - travel to meetings related to activity for the NAHLN, assist other laboratories during emergencies, utilize the APHIS laboratory portal; 4. Purchase equipment that directly increases laboratory testing capacity & maintain equipment service agreements - replace aging equipment to improve capability and efficiency; 5. Electronic messaging enhancement - maintain and enhance electronic messaging for disease reporting and adhere to HL7 requirements; 6. Support APHIS- and NAHLN- related activities in the establishment of technical aspects of emerging disease detection and reporting protocols.

Impacts
What was accomplished under these goals? As a NAHLN Level 1 laboratory, the Ohio ADDL supports the NAHLN by providing the capabilities and capacities to support early detection, rapid response, and appropriate recovery from high-consequence animal diseases. In addition to supporting the NAHLN mission, the Ohio ADDL offers testing for regulatory diseases and elective diagnostics for veterinarians in Ohio and beyond. In 2022, the Ohio ADDL completed 453,135 tests; as of 12/21/2023, the Ohio ADDL has completed 474,303 tests in 2023. ADDL utilizes USALIMS v.2022.002. NIFA NAHLN Infrastructure funds are used to support the annual contract with Acclaim, the vendor of USALIMS. ADDL participated in several NVSL and NAHLN proficiency tests. All tests were successfully completed. Our yearly subscription for Q-Pulse is supported using this NIFA NAHLN Infrastructure funding. Q-Pulse maintains lab quality system records like standard operating procedures and associated worksheets, external documents, the lab quality manual, training records, and equipment asset records. NIFA NAHLN Infrastructure supported travel for lab leadership to attend the annual AAVLD meeting. New barcode printers and scanners were purchased to support high volume testing, particularly during the Ohio HPAI incident. In 2022, the Ohio ADDL completed 5,029 avian influenza PCR tests; as of 12/21/2023, the laboratory has completed 9,296 avian influenza PCR tests. The majority of the 2023 avian influenza PCR tests have been completed in November and December as part of the Ohio HPAI incident that involves 5 infected premises and their associated control areas and surveillance zones. The NIFA NAHLN Infrastructure funds support service contracts for critical lab equipment including biosafety cabinets, the digester, pipettes, autoclaves, and real-time PCR instruments. The Ohio ADDL interacted several times with the new Unknown Morbidity and/or Mortality Event (UME) group regarding cases with an unknown, possibly infectious etiology.

Publications