Source: OREGON STATE UNIVERSITY submitted to
FURTHERING THE DEVELOPMENT OF SPROUT INHIBITORS AND GROWTH SUPPRESSANTS FOR ORGANIC POTATO
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
OTHER GRANTS
Reporting Frequency
Annual
Accession No.
1027247
Grant No.
2021-51106-35584
Cumulative Award Amt.
$595,000.00
Proposal No.
2021-04812
Multistate No.
(N/A)
Project Start Date
Sep 1, 2021
Project End Date
Aug 31, 2025
Grant Year
2021
Program Code
[112.E]- Organic Transitions
Project Director
Jeliazkov, V.
Recipient Organization
OREGON STATE UNIVERSITY
(N/A)
CORVALLIS,OR 97331
Performing Department
Crop and Soil Science
Non Technical Summary
This project will address an important industry issue related to organic potato storage and control of premature sprouting. We hypothesized that plant natural products such as essential oils (EO), EO fractions, blends, and individual compounds can be discovered that will have greater sprout suppression activity compared with the currently used sprout suppressors/inhibitors in organic potato. The long-term goal is to develop potato sprout suppression/inhibitor product(s) for organic potato. The objectives are: (1) Screening of ~ 300 EO and EO fractions and identify ones with the highest potato sprout suppression; (2) Bioassay-guided fractionation of selected EO and EO fractions; (3) Compare the most promising compounds and combinations against currently used sprout inhibitors; (4) Reveal the mode of action of the promising compounds and blends on potato tubers by employing transcription profiling and metabolomics approaches; (5) Communicate the results to the potato industry, scientists, extension, students, and general public. Incorporate the information into online undergraduate and graduate certificates in Organic Agriculture to reach out to students across the nation. The project goal and objectives are in line with the FY 2021 Priority Areas, specifically Priority 3: "Develop cultural practices and other allowable alternatives.." and Priority 4: "Overcome barriers to organic transition". The project will address limitations in organic potato storage and sprout control that also hinders transition of conventional potato producers to certified organic. The results will facilitate the development of new potato sprout suppressors or inhibitors, improve the quality of potato, reduce environmental impact, and contribute to improved human health.
Animal Health Component
75%
Research Effort Categories
Basic
25%
Applied
75%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20313101060100%
Knowledge Area
203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants;

Subject Of Investigation
1310 - Potato;

Field Of Science
1060 - Biology (whole systems);
Goals / Objectives
The goal is to discover essential oil (EO) fractions, blends, and individual compounds that will have greater sprout suppression activity compared with the currently used potato sprout suppressors or inhibitors. The long-term goal is to develop potato sprout suppression/inhibitor product(s) to be used for extending dormancy and/or suppression of premature sprouting in transitional and certified organic potato production. This will overcome one of the major barriers to organic transition faced by potato growers. The development of new sprout suppressors for organic potato will improve the quality of potato and reduce waste from storage, reduce the environmental impact of chemical inhibitors, and contribute to improved human health. The project is based on discussions with potato growers, and will have positive impact on transitional and certified organic production, will improve access to additional markets and consumer groups, and it will increase the competitiveness of the U.S. potato industry.The objectives are: (1) Screening of ~ 300 EO and EO fractions and identify ones with the highest potato sprout suppression; (2) Bioassay-guided fractionation of selected EO and EO fractions; (3) Compare the most promising compounds and combinations against currently used sprout inhibitors; (4) Reveal the mode of action of the promising compounds and blends on potato tubers by employing transcription profiling and metabolomics approaches; (5) Communicate the results to the potato industry, scientists, extension, students, and general public. Incorporate the information into online undergraduate and graduate certificates in Organic Agriculture to reach out to students across the nation.
Project Methods
Objective 1: Screening of 300 natural products (EO, EO fractions, hydrosols, and extracts) that were not tested previously, and identify ones with the highest potato sprout suppression for further bioassay-guided fractionation.Phase 1. Screening of essential oils (EO) and EO fractions in 15-L containersPhase 1.1. First group of controlled environment experiments: Two potato varieties representing fresh market & processing will be used for this group of experiments. A detailed screening of around 300 EOs and EO fractions will be conducted in 15-L containers. A randomized complete design with three replications will be used to evaluate sprouting. Water, Biox (clove oil) and Biox-M (spearmint oil) will be used as controls in all experiments. Measurements of sprouts will be taken after 15, 30, and 45 days.Phase 1.2. Second group of controlled environment experiments: The EO that showed potential as sprout inhibitors will be fractioned (in 4-5 distillation fractions per EO) in Dr. Jeliazkov's laboratory at OSU. Both the EO and the EO fractions will be analyzed for chemical profile using Gas chromatography/Mass spectroscopy (GC/MS) by Dr. Charles L. Cantrell at the National Center for Natural Products Research at the University of Mississippi. We will be using the same controls, water, Biox, and Biox-M and the same method outlined in Phase 1.1.Phase 1.3. Third group of controlled environment experiments: The most effective EO, EO fractions, and pure compounds for suppressing sprouting from the previous groups of experiments will be evaluated at different concentrations and duration will be studied by applying selected oils every 15 or 30 days in storage by using wick application. In addition, the selected EO and EO fractions will be tested at T of 8 °C. Statistical regression models will be developed that may predict the best concentration and duration of treatments for the selected oils and compounds.Microscopy analyses of the tuber sprouts to test the hypothesis that most (but not all) EOs (1) may induce necrosis of the meristematic cells but not on the potato periderm; and (2) may break the apical dominance and induce axillary bud sprouting. Tubers from the most contrasting treatments will be examined under light and Scanning Electron Microscopes (SEM) in order to determine if the observed effects are due to physical damage of potato sprouts.Objective 2: Bioassay-guided fractionation of the EOs and EO fractions generated in activities outlined in Objective 1.Bioassay screening results will always dictate the progression of which EO to evaluate and/or which fraction to use for continuation of purification. In general, the most biologically active EO's will be chosen for fractionation. Once fractions are generated, biological evaluation will be repeated.Objective 3: Compare the most promising natural product combinations against Biox® and Chlorpropham (CIPC) in a semi-commercial facility.This will screen the most promising EO, EO fractions and pure compounds in 55-gallon (200-L) semi-commercial vessels.Objective 4: Studies to reveal the mode of action of the identified most promising EOs on potato tubers. Integration of state-of-the art methodologies to determine effects of exogenous treatments that alter natural dormancy progression can help identify critical molecular, biochemical and physiological mechanisms, and ultimately, pinpoint the regulatory genes and pathways that can be used as novel targets for manipulation of tuber dormancy more efficiently. Transcriptome Profiling: Tubers will be used to collect samples (i.e., primary buds) during postharvest storage (at different time points following the treatments). Each sample will contain 50-100 buds. RNA will be extracted from isolated meristems using a commercial kit (e.g., Qiagen, RNeasy Plant Mini Kit) for all treatments, biological replicates and time points. RNA concentration and quality will be quantified spectrophotometrically, and RNA integrity will be determined with an Agilent Technologies 2100 Bioanalyzer. Libraries of cDNAs (Illumina) will be generated from RNA samples and sequenced. RNA-seq data will be processed and mapped to the reference potato genome. Differentially expressed genes will be identified and classified by function, and overrepresented ontologies among biological pathways and molecular mechanisms will be identified (e.g., gene-set enrichment and sub-network enrichment analyses). RNAseq results will be confirmed by RT-PCR to quantify transcript abundance of a subset of genes. Targeted metabolomics analysis of plant growth hormones. This will be conducted in the Co-PI Dr. Fred Stevens' laboratory at OSU. We will apply ultra-performance liquid chromatography (UPLC) with triple-quadrupole linear ion trap mass spectrometry for their analysis. We will detect and quantify growth hormones on a Sciex 4000 QTrap instrument using a selected reaction monitoring (SRM) method developed in Dr. Stevens' laboratory. Our current panel of plant hormones includes abscisic acid (+/- ABA), ABA-glucopyranosyl ester, dihydrophaseic acid, phaseic acid, neophaseic acid, 7'-hydroxy-ABA, indole-3-acetic acid (IAA), IAA-alanine, IAA-aspartate, IAA methyl ester, (cis/trans)-zeatin, (cis/trans)-zeatin riboside, dihydrozeatin (DZ), DZ riboside, isopentenyladenine, isopentenyladenosine, and several gibberellins (GA1, GA3, GA4, and GA7).Untargeted metabolomics analysis of plant tissues. The acidified water-methanol extracts will also be analyzed on our untargeted plant metabolomics platform consisting of a UPLC quadrupole Time-of-Flight mass spectrometry system (Sciex 5600 QToF MS/MS) to semi-quantify secondary metabolites, such as steroidal alkaloids and their glycosides.Objective 5: Extension and Education: Communicate the results to the potato industry, scientists, extension, and general public. Incorporate the information from this project into online courses of the OSU online undergraduate and graduate certificates in Organic Agriculture.The results from this project will be disseminated through (1) two factsheets, (2) a recorded webinar for eOrganic, (3) an article at eOrganic, (4) a project website, (5) at growers meetings, and field days, (6) articles in scientific journals, (7) incorporating the new knowledge in two Online Certificates on Organic Farming at OSU. Project participants will be presenting at OR Potato Growers meetings and at the potato field days every year. As part of the potato field days at the OSU HAREC, we will be offering a short workshop on potato sprout control using natural products. To reach out to growers, processors, and general public, the project participants will develop and record a webinar. The recorded webinar will be archived on eOrganic (https://eorganic.info/ ) website and on the eOrganic YouTube channel at https://www.youtube.com/eorganic . We discussed it with Alice Formiga (eOrganic director), Horticulture Dep. at OSU, and she will be helping with seminar presentation and posting on eOrganic. A link to the recorded webinar will be provided from the website of OR Potato Commission (https://www.oregonspuds.com/)In addition, the results will be compiled in at least 3 manuscripts and published in refereed scientific journals.The results will be included in teaching materials for OSU face to face and online courses. OSU has recently launched an Undergraduate Certificate in Organic Farming, and an Online Graduate Certificate in Organic Agriculture. The knowledge gained within this project will be incorporated in online courses in the Organic Certificates to reach students and professionals across the nation.

Progress 09/01/22 to 08/31/23

Outputs
Target Audience:We engaged with potato growers through a presentation at the Hermiston Farm Fair in December 2022, reaching approximately 150 participants, as well as with members of the Oregon Potato Commission in March 2022. Additionally, my graduate students and I presented our findings at various international conferences, attended by a diverse audience of professionals, extension agents, and researchers. These events provided valuable opportunities to share our work and foster discussions within the broader agricultural community. Below please find the exact presentations. The graduate student names are noted with asterisk: Jeliazkov (Zheljazkov), V.D. and J. Thoma*. 2022. Title: The promise of essentials oils as sprout suppressants in potato storage and as alternatives to synthetic pesticides. The 49th Hermiston Farm Fair Seminars, Nov 30 - Dec 2, 2022. Invited presentation, approximately 150 participants. https://agsci.oregonstate.edu/harec/farm-fair Jeliazkov (Zheljazkov), V.D. 2022. Title: Plant natural products as sprout inhibitors or growth suppressants in potatoes. Presented at the Oregon Potato Commission Meeting, March 3, 2022. Approximately 15 participants. Zheljazkov, V.D. Invited presentation. 2023. Essential Oils for Sprout Control in Potato. 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023, May 30-02 June 2023, Sts. Constantine and Helena Resort, Bulgaria. Approximately 180 participants. https://icnpu2023.com/ Almutairi*, H., V.D. Zheljazkov, and C.L. Cantrell. 2023. Essential Oils for Potato (Solanum tuberosumL.) Sprout Suppression at Room Temperature.Association for the Advancement of Industrial Crops, (AAIC)-2023, 34thAnnual Meeting, Aug 27-30th, Corvallis, OR, USA. Approximately 60 participants. https://aaic.org/2023-annual-meeting/ Alzarqaa*, A. and V.D. Zheljazkov. 2023. Efficacy of Essential Oils in Suppressing Potato (Solanum tuberosumL.) Sprouting. Association for the Advancement of Industrial Crops, (AAIC)-2023, 34thAnnual Meeting, Aug 27-30th, Corvallis, OR, USA. Approximately 60 participants. https://aaic.org/2023-annual-meeting/ Changes/Problems:We do not anticipate any major challenges. What opportunities for training and professional development has the project provided?The project enhanced graduate students' and participants' understanding of potato sprout control through hands-on research and practical application. Graduate students gained valuable experience in data analysis and scientific communication by compiling their findings into a manuscript for publication. This process not only deepened their technical expertise but also provided critical training in scientific writing and presentation, equipping them with skills essential for their professional development. How have the results been disseminated to communities of interest?We've been actively sharing our research on potato sprout control with growers and industry experts. In December 2022, we presented our work at the Hermiston Farm Fair, connecting with about 150 attendees, and then in March 2022, we engaged with members of the Oregon Potato Commission. These events allowed us to bring our findings directly to the people who could benefit most. We've also taken our research to the international stage. My graduate students and I presented at several major conferences, where we connected with professionals, extension agents, and researchers from around the world. These interactions not only let us share our discoveries but also sparked valuable discussions within the agricultural community. Here's a rundown of our presentations (graduate student names are marked with an asterisk): Jeliazkov (Zheljazkov), V.D. and J. Thoma*. 2022. The promise of essential oils as sprout suppressants in potato storage and as alternatives to synthetic pesticides. Presented at the 49th Hermiston Farm Fair Seminars, Nov 30 - Dec 2, 2022, reaching about 150 participants. Hermiston Farm Fair Jeliazkov (Zheljazkov), V.D. 2022. Plant natural products as sprout inhibitors or growth suppressants in potatoes. Shared at the Oregon Potato Commission Meeting on March 3, 2022, with around 15 attendees. Zheljazkov, V.D. 2023. Essential Oils for Sprout Control in Potato. Delivered at the 5th International Conference on Natural Products Utilization in Bulgaria, May 30 - June 2, 2023, to an audience of roughly 180 participants. ICNPU-2023 Almutairi, H., V.D. Zheljazkov, and C.L. Cantrell*. 2023. Essential Oils for Potato (Solanum tuberosum L.) Sprout Suppression at Room Temperature. Presented at the AAIC-2023, 34th Annual Meeting, Aug 27-30, in Corvallis, OR, to around 60 participants. AAIC-2023 Alzarqaa, A. and V.D. Zheljazkov*. 2023. Efficacy of Essential Oils in Suppressing Potato (Solanum tuberosum L.) Sprouting. Also presented at the AAIC-2023, with about 60 attendees. AAIC-2023 Through these events, we've been able to share our work with a diverse audience and engage in discussions that will help shape the future of sustainable sprout control in potato storage. What do you plan to do during the next reporting period to accomplish the goals?· Ongoing Analysis: Continue analyzing potato tissue samples using HPLC/MS to monitor plant hormones, glucose, and abscisic acid levels, evaluating changes in potato quality under essential oil treatments. · Chemical Profiling: Further analyze the chemical profiles of the most effective essential oils identified in the project, focusing on optimizing their use in potato sprout control. · Fractionation and Compound Identification: Conduct fractionation of the most effective essential oils to isolate specific fractions and compounds, paving the way for new product development. · Controlled Environment Studies: Set up additional controlled environment experiments to further test and refine the most effective essential oil treatments identified to date. · Essential Oil Fraction and Blend Studies: Expand studies on essential oil fractions and blends to determine optimal combinations for sprout control efficacy. · Outreach and Presentations: Present findings at the Hermiston Farm Fair. · Industry Collaboration: Continue the collaborative work with the 1,4 Group utilizing their facilities in Meridian, Idaho · Publications: Compile and prepare project findings for submission, targeting 1-2 refereed journal publications in 2023-2024.

Impacts
What was accomplished under these goals? ·Completed analysis of potato tissue samples for sugars using NMR for some of the experiments. · Analyzed the chemical profile of the most effective essential oils identified in some of the experiments. · Set up and conducted follow-up controlled environment experiments with the most effective essential oils discovered so far. · Conducted detailed studies on essential oil fractions and blends for their effectiveness. · Presented findings at the Hermiston Farm Fair on December 2, 2022. · Graduate students presented seminars on their research progress and findings. · Successfully defended MS thesis by Jena Thoma, a graduate student contributing to this project. · Held a collaborative meeting with the 1,4 Group to explore joint efforts in developing alternative sprout control products for potatoes. · Compiled project results into two refereed journal publications submitted in 2023.

Publications

  • Type: Peer Reviewed Journal Articles Status: Published Year Published: 2023 Citation: 0000-0002-3479-9653 Thoma JL, Cantrell CL, Tamang P and Zheljazkov VD (2023) Determining the optimum mixture of three essential oils for potato sprout suppression at room temperature storage. Front. Plant Sci. 14:1199117. doi: 10.3389/fpls.2023.1199117


Progress 09/01/21 to 08/31/22

Outputs
Target Audience:Target audiences include researchers, graduate students, extension agents, growers, processors, other agricultural professionals, and general public. Efforts: We have been in contact with Oregon Potato Association and presented at the meeting at the Oregon Potato Commission. Also, our extension efforts include the current development of extension publication, development of an webinar to be presented at eOrganic, and presentation at international conference. In addition, we shared the research results so far with the scientific community and graduate students by 4 refereed journal publications. Refereed journal publications: Zheljazkov, V.D., G. Micalizzi, S. Yilma, C.L. Cantrell, A. Reichley, L. Mondello, I.B. Semerdjieva, and T. Radoukova. 2022. Melissa officinalis L. as a Sprout Suppressor in Solanum tuberosum L. and an Alternative to Synthetic Pesticides. J. Agric. Food Chem. 2022, 70, 44, 14205-14219 https://doi.org/10.1021/acs.jafc.2c05942 Impact factor (IF): 5.8 Thoma, J.L.; Cantrell, C.L.; Zheljazkov, V.D. 2022. Effects of Essential Oil Fumigation on Potato Sprouting at Room-Temperature Storage. Plants 2022, 11, 3109. https://doi.org/10.3390/plants11223109 IF: 4.65 Thoma, J.L.; Cantrell, C.L.; Zheljazkov, V.D. 2022. Evaluation of Essential Oils as Sprout Suppressants for Potato (Solanum tuberosum) at Room Temperature Storage. Plants, 11, 3055. https://doi.org/10.3390/plants11223055 IF: 4.65 Thoma, J.; Zheljazkov, V.D. 2022. Sprout Suppressants in Potato Storage: Conventional Options and Promising Essential Oils--A Review. Sustainability 2022, 14, 6382. https://doi.org/10.3390/su14116382 Impact Factor: 4.08 ?Conference presentations Zheljazkov, V.D., G. Micalizzi, S. Yilma, L. Mondello, and C.L. Cantrell. 2021. Essential oils as sprout inhibitors in potato. Oral presentation. Symposium "Hemp, Medicinal and Aromatic Crops: Production, Phytochemistry, & Utilization" American Chemical Society, ACS 2021 Fall Meeting. Atlanta, GA; August, 22-26, 2021 Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Jeliazkov, the PI of the project hired four graduate students that are being trained and conduct research on the project goals and objectives. All of the students are minorities, and 3 are international. Three of the students have stipends from the Saudi Arabia government. These include: Name PhD/MS Title Current position Luluh Nasser A Aljayan MS Natural products for potato sprout control MS Jena Thoma MS Essential oils as sprout inhibitors for potato MS Hanin Mukhlid M Almutairi PhD Studies on essential oils as sprout inhibitors in potato (tentative) PhD student Areej Abdulah A. Alzarqaa PhD Screening of natural products for sprout control in potato (tentative) PhD student How have the results been disseminated to communities of interest?As indicted in the previous sections, our group already published 4 refereed journal papers in 2022. These are reaching researchers, graduate students, and extension agents across the country and internationally. In addition, we are reaching out to growers, e.g. with a presentation at the Oregon Potato Commission Meeting in March, 2022. What do you plan to do during the next reporting period to accomplish the goals?The four graduate students, I, and the other project participants will continue with the research work as outlined in the project proposal to meet the objectives. These include the following activities: Continue with the analyses of potato tissue samples using HPLC/MS for plant hormones and glucose and abscisic acid kits to evaluate potato quality changes as a function of the essential oil treatments Continue with the analyses of chemical profile of the most effective essential oils identified in this project so far Fractionation work on the most effective essential oils to identify fractions and individual compounds (molecules) that can be used for new product development Continue with setting up follow up controlled environment experiments with the most effective essential oils identified so far Essential oil fractions and blends studies Presentation at the 2022 Hermiston Farm Fair on Dec 2, 2022 Seminar presentations of the graduate students on their research Defense of MS thesis by Jena Thoma, graduate student working on this project Meeting with 1,4 group that is developing alternative sprout control products for potato to develop collaborative efforts for new product development Compile our results so far into 2-5 refereed journal publications in 2023 Develop an Extension publication Present a Webinar for eOrganic

Impacts
What was accomplished under these goals? First, we did a very detailed literature search and compiled the information into a review paper. This review presented an overview of physical and chemical means of sprout suppression in stored potato and critically analyzed studies focusing on the use of essential oils (EOs) for sprout suppression. Challenges and limitations of EO use in potato sprout suppression are discussed as well as areas of future research. The review was published in Sustainability in 2022. We conducted around 20 different controlled environment experiments so far to meet the objectives. We did fractionation of one of the most promising essential oils (EO) from the first two experiments, tested these fractions, and individual pure compounds found in these fractions. The EOs of interest were analyzed using gas chromatography coupled to mass spectrometry (GC-MS) and/or a flame ionization detector (GC-FID); the detailed chemical profiles was provided. The Melissa officinalis EO was fractionated into 7 fractions and these were tested on minitubers. We identified two fractions (F and A) that suppressed potato sprouting better than the whole oil. The GC-MS-FID analyses of M. officinalis EO fraction A identified myrcene, Z-ocimene, E-ocimene, trans-caryophyllene, and α-humulene as the main constituents, while the main constituents of fraction F were α-terpineol, β-citronellol, and geraniol. The pure isolated compounds, together with the major compound in M. officinalis EO (citral), were tested for sprout suppression on 3 potato cultivars (Ranger Russet, Terra Rosa, and Dakota TrailBlazer), which revealed that β-citronellol reduced the sprout length and the number of sprouts in all 3 cultivars, while citral and (+)-α-terpineol) reduced the sprout length in all 3 cultivars and the number of sprouts in Ranger Russet relative to the two controls. Myrcene had stimulating effect on the number of sprouts in Terra Rosa. However, none of the pure compounds suppressed sprouting completely or comparable to the EO of M. officinalis. In one of the graduate student experiments, we evaluated twenty-one essential oils (EOs) as potential sprout suppressants in cv. Ranger Russet potatoes at room-temperature storage. Treatment with Artemisia herba-alba EO was the most effective at suppressing both sprout length and sprout number over a 90-day storage period. GC--MS--FID analysis of A. herba-alba EO revealed the presence of α-thujone, hexadecenoic acid, β-thujone, camphor, sabinene, and camphene at amounts >1%. Other plants such as Cistus ladanifer, Ocimum basilicum, Ormenis mixta, and Salvia sclarea EOs significantly reduced sprout length for shorter storage periods, whereas Cinnamomum zeylanicum (bark) and Laurus nobilis EOs also significantly reduced sprout number. Syzygium aromaticum (clove) EO did not significantly suppress sprouting at room temperature. These results indicate the potential of certain EOs to be used as sprout suppressants for room-temperature potato storage, providing needed alternatives for both organic and conventional potato industries. The findings were compiled into a manuscript and published in the journal Plants in 2022. Yet, in another graduate student experiment, we evaluated the potential of ten EOs for sprout suppression in potato cultivar Ranger Russet at room temperature. Treatment with Cymbopogon citratus EO was found to be the most effective sprout suppressant, completely suppressing sprouting over the 90-day storage period. The EOs of Myrtus communis and Melaleuca quinquenervia significantly reduced sprout length relative to the control but did not have any effect on sprout number. The findings were compiled into a manuscript and published in Plants in 2022.

Publications

  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Thoma, J.L.; Cantrell, C.L.; Zheljazkov, V.D. 2022. Effects of Essential Oil Fumigation on Potato Sprouting at Room-Temperature Storage. Plants 2022, 11, 3109. https://doi.org/10.3390/plants11223109 Impact Factor: 4.65
  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Zheljazkov, V.D., G. Micalizzi, S. Yilma, C.L. Cantrell, A. Reichley, L. Mondello, I.B. Semerdjieva, and T. Radoukova. 2022. Melissa officinalis L. as a Sprout Suppressor in Solanum tuberosum L. and an Alternative to Synthetic Pesticides. J. Agric. Food Chem. 2022, 70, 44, 1420514219 https://doi.org/10.1021/acs.jafc.2c05942 Impact factor (IF): 5.8
  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Thoma, J.L.; Cantrell, C.L.; Zheljazkov, V.D. 2022. Evaluation of Essential Oils as Sprout Suppressants for Potato (Solanum tuberosum) at Room Temperature Storage. Plants, 11, 3055. https://doi.org/10.3390/plants11223055 IF: 4.65
  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Thoma, J.; Zheljazkov, V.D. 2022. Sprout Suppressants in Potato Storage: Conventional Options and Promising Essential OilsA Review. Sustainability 2022, 14, 6382. https://doi.org/10.3390/su14116382 Impact Factor: 4.08