Progress 09/01/21 to 08/31/22
Outputs Target Audience:The goal of this SBIR effort is to contribute value and ingredient diversity to the food industry. The general interest in insects as a sustainable protein source is booming. Over 80 companies in North America and Europe currently offer a wide variety of food products with insects as a key ingredient (Dossey et al., 2016a; Dossey et al., 2016b). In fact, All Things Bugs LLC has led the industry's development of insects as a protein source, selling over 15,000 pounds of our Griopro® brand cricket powder. However, for this industry to be successful, we must continue to innovate and diversify. Mealworms (the larval stage of the Yellow Mealworm beetle species Tenebrio molitor), being the second most produced insect in North America (and most produced in Europe) represent the logical next step in our R&D. Small farm customer validation: Our research has discovered that there is a very large emerging interest in mealworm farming. Hundreds of small hobbyist farmers are planning to start their own commercial mealworm farms. The large capital raises (i.e. Ynsect - $400M and Beta Hatch - $9M) among mealworm companies validates our concept and choice of mealworms as a target species! We have surveyed over 20 small and mid-sized mealworm farms during Phase I to determine their needs as farmers, what aspects of their operation could be improved via technology and what their major cost drivers are. All have provided enthusiastic feedback that our focus on automated growth trays and mealworm harvesting are EXACTLY what they need to scale their operations and make them more profitable! Changes/Problems:The problems we have encountered to date are primarily due to global supply chain delays of equipment parts and materials via our sub-contractor. The sub-contractor building our equipment (Robotray in Miami, FL, USA) has also experienced what many companies in the US and world-wide have experience during the pandemic: labor shortages. Additionally, we have made some substantial improvements to our mealworm farming system (see details described above and below) which are not problems but did require us to adjust our schedule and strategy a bit. Nonetheless, we have made all of these adjustments and thus have remained largely on- schedule with a much better improved project. Also, our lab mealworm incubator experienced humidity control failure which caused us to lose many mealworms in ongoing experiments due to low humidity levels. We have found alternative ways to control humidity and can also use our walk-in environmental chamber currently used for crickets. We are currently shopping for a new reach-in mealworm incubator and searching for funds to buy one. In addition to delays in getting the equipment from our subcontractor, our senior scientist at All Things Bugs LLC left the firm at the beginning of the project for another job, so we had to replace him with the best alternative mealworm care technician we could find. We were able to find and train the new technician quickly and he is now operating fully independently in the laboratory with the mealworm rearing experiments. Thus in Year 2, we will complete the mealworm egg harvesting and mealworm feed optimization experiments in Objective 3 toward writing a mealworm farming manual for future small mealworm farm clients and the larger food and feed industry. What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?Publications, Presentations and Other Publicized Work Products (no max pages) Selected Subset of Presentations and Abstracts: 2022: As with the rest of the world, the various effects of the COVID-19 pandemic (reduced ability to travel, cancelled conferences, budget constrains and overall business uncertainty) has meant we have not had as many public presentations or publications as would have been typical in the past 1-2 years, or that we would have liked. However we were able to do the following: * In 2022 and 2023 we have several major publications and presentations of our substantial ground breaking work coming out on cricket and mealworm genetics, genomics, genetic engineering, farming innovations and food product development. Additionally, we have significant PR and marketing campaigns to launch in 2023 as well as at least 1 major new food product launch (cricket based high protein breakfast cereal). PATENT ISSUED: Aaron T. Dossey, INSECT PRODUCTS AND METHODS OF MANUFACTURE AND USE THEREOF, United States, U.S.?Patent Number: 11,337,451; U.S. Patent Application Number: 14/537,960; International Patent Application Number: PCT/US14/64920. PATENT ISSUED: Aaron T. Dossey, INSECT PRODUCTS AND METHODS OF MANUFACTURE AND USE THEREOF, Canada, Patent Application Number: 2,929,177; National Stage Entry of International Patent Application Number: PCT/US2014/064920. Advances in Genome Biology and Technology (AGBT); Agriculture Meeting (first ever agriculture meeting for AGBT); April 4-6, 2022; Lowes Coronado Bay Resort, Coronado Island, San Diego, CA, USA; Selected as a "Flash Talk"; Monday, February 24, 2020; Title: " Cutting Edge Genomics and Engineering Insects for Sustainable Food, Feed and Pharma". Dr. Aaron T. Dossey and Dr. Clay Chu. Room: Wednesday, April 6, 2022, held in the Commodore Ballroom from 10:20am - 10:40am. Advances in Genome Biology and Technology (AGBT); Agriculture Meeting (first ever agriculture meeting for AGBT); April 4-6, 2022; Lowes Coronado Bay Resort, Coronado Island, San Diego, CA, USA; Poser# 803; Title: " Cutting Edge Genomics and Engineering Insects for Sustainable Food, Feed and Pharma". Dr. Aaron T. Dossey and Dr. Clay Chu. Room: Exhibit Hall & Sponsor Promenade/ Avalon. What do you plan to do during the next reporting period to accomplish the goals?We plan to continue execution of our objectives into year 2.
Impacts What was accomplished under these goals?
To date this project, while delayed, has been quite successful and we have made some very valuable and helpful discoveries. Our work has also generated a lot of public interest, several publications and presentations etc. The following lists our discoveries, successes, achievements, publications and presentations to date: Discoveries and Successes: Our most significant discovery and innovation to date has been Dr. Dossey's re- design of the mealworm farming, harvesting handling, separating and processing system. His invention to leverage automated bakery/food industry equipment, particularly the tray handling and emptying system to unload standard industry trays from racks or stacks, dump them onto a conveyor, into a hopper or directly into a shaker sifter, then return them to a new or the original rack or stack, is a game changing innovation that will substantially improve the efficiency of automated mealworm farming at any scale, even for single-operator small farmer startups. This innovation leverages and adapts existing equipment and accessories (trays/racks etc.) used in the bakery, food and/or insect rearing industries to substantially improve mealworm farming for greater efficiency and lower cost. It uses bakery trays and racks or more modern stackable specialized mealworm/insect growth trays (used for other insects too like black soldier fly larvae, superworms, buffalo worms etc.). However, we are adding features that fully automate the process. We have discovered that humidity is critical for mealworm, growth and, while mealworms can grow in substrate at any humidity level above 25% without any water added, at dryer conditions growth is very slow and inefficient. Additionally, we have shown a substantially higher mortality and lower survival rate at lower humidity levels (survival from egg to harvest as low as 30%). However, at humidities over 50%, ideally at 70-80%, mealworms thrive in wheat bran or rice bran, and survival is well over 50% (can be as high as 75% in ideal optimal conditions). Adding water via water crystals (polyacrylamide or cut up vegetables) is not nearly efficient, requiring lots of labor and risk of mold growth. We also demonstrated that even at 70-80% humidity, there is little or no mold growth in mealworm substrates during a growth cycle, thus limiting risk of mealworm die-off or contamination of mold or mycotoxins in mealworms grown for human food or animal feed. This experiment is very valuable, as it demonstrates our concepts of using humidity in an efficient, hands-off fully-automated mealworm production facility are valid and can work. We have also discovered that rice bran substantially out-performs wheat bran as a mealworm feed. We found growth rates and survival as high as double for mealworms grown on rice bran vs wheat bran. This is great news for many reasons, especially that growing mealworms on rice bran results in mealworm protein that is gluten-free which is more desirable for the food industry. Also, rice bran appears to result in healthier mealworms which are more resistant to dryer conditions (higher survival in general, even when humidity is lower). These were all preliminary experiments and we plan to scale them up for more robust data in Year 2. During Year 1 we also conducted preliminary experiments to measure the number of mealworm eggs ideal to add per kilogram of mealworm feed (currently wheat bran) to optimize growth and survival while minimizing feed waste and eliminating the need to feed mealworms during a growth cycle (eg: add feed only at the beginning then "set and forget" until harvest - also the goal of using humidity vs added water). We were very surprised and pleased to discover that we under-estimated the number of mealworms and eggs that a kilogram of feed could support by approximately half. Thus, we should be able to produce approximately double or more the kilograms of mealworms and protein per kilogram of mealworm feed originally estimated under optimal temperature and humidity conditions. Additionally, we found literature showing that mealworms grow best in full time dark (eg: no light) conditions which also supports mealworms as an efficient, sustainable low cost and low-resource intensive protein source since there is no need to supply them with light at any growth stage. We will verify these results and scale up these experiments in year 2.
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2022
Citation:
5) Advances in Genome Biology and Technology (AGBT); Agriculture Meeting (first ever agriculture meeting for AGBT); April 4-6, 2022; Lowes Coronado Bay Resort, Coronado Island, San Diego, CA, USA; Selected as a Flash Talk; Monday, February 24, 2020; Title: " Cutting Edge Genomics and Engineering Insects for Sustainable Food, Feed and Pharma". Dr. Aaron T. Dossey and Dr. Clay Chu. Room: Wednesday, April 6, 2022, held in the Commodore Ballroom from 10:20am - 10:40am.
|