Source: MAINE SHELLFISH DEVELOPERS LLC submitted to
DEVELOPMENT AND EXPANSION OF CLOSED CONTAINMENT OYSTER FEEDLOTS AT NON-COASTAL SITES
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1024090
Grant No.
2020-33610-32516
Cumulative Award Amt.
$649,690.00
Proposal No.
2020-07054
Multistate No.
(N/A)
Project Start Date
Sep 1, 2020
Project End Date
Aug 31, 2022
Grant Year
2020
Program Code
[8.12]- Small and Mid-Size Farms
Project Director
Pryor, T.
Recipient Organization
MAINE SHELLFISH DEVELOPERS LLC
193 CLARKS COVE R
WALPOLE,ME 045733307
Performing Department
(N/A)
Non Technical Summary
The Value Proposition: Our proposed land-based, shellfish feedlots will provide customers large, fat, tasty oysters - and eventually clams, mussels and perhaps scallops -- at a reasonable price safely and sustainably. Controlled growth in large quantities could open additional value-added and export opportunities.Our vision and commitment is that MSD shellfish harvests meet Food and Agriculture Organization's (FAO)'s classic "four pillars of food security": 1) Physical availability (the supply side as determined by the level of production), 2) Economic and physical access (meets everyman's budget), 3) Food utilization (highly palatable and highly nutritional), 4) Stability (assuring that the above three are always easily accessible). (World Food Summit 1996)To this list add non-seasonal, traceable and sustainable (thanks to aquaponics) with zero waste. With controlled conditions and an inexpensive feed, the cost per oyster will be substantially lower than the same oyster farmed offshore where in Maine it's typically around forty-two cents. MSD envisions licensed oyster feedlots positioned strategically across the USD, providing low cost oysters utilizing a remote feedlot management process and the MSD provided feed and seedlings.
Animal Health Component
30%
Research Effort Categories
Basic
30%
Applied
30%
Developmental
40%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
70208111010100%
Goals / Objectives
MSD's goal is to develop palatable, nutritional and inexpensive feed in high volume and densely supplied 24/7 year around to oysters grown in the temperature-controlled Mark V feedlot. Success in developing this alternate feed will permit year-round growth and sale of oysters readily available to all, disrupting the traditional business model for shellfish farming with potential beneficial global impact.
Project Methods
Task 1 -- Yeast autolysis: MSD will use an established industrial process whereby we heat the spent yeast to 50° C until the yeast undergoes autolysisTask 2 - Cell wall separation: After autolysis, the spent cell walls of the yeast will be separated from the lysate.Task 3 - Measuring/balancing parameters: Following separation, we will assess the density of the yeast autolysate, or yeast extract, by measuring Total Dissolved Solids (TDSTask 4 -- Dewatering: In pursuit of dense algal cultures, MSD will experiment with different extract dewatering techniques, focused on maintaining extract quality and low cost.Task 5 - Measuring effectiveness: The team will evaluate the effectiveness and economy of the yeast extract as both a cell culture component and biostimulant. Cultures will be tested for cell density and nutritional composition.Task 6 - Dry weight minimum: We will then evaluate the resulting density with our automated cell counter. In this stage we aim to achieve a minimum of 10g/l of cell dry weight before moving on to a fermenter unit, with continuous optimization thereafter.Task 7 - Establishing protocols: Filtered media (the water around the cells) from the cultures will be tested for carbon, nitrogen and phosphorus.Task 8 -Production scale-up: Simultaneously, the technical team with strategic partner OO with bulk production capacity will be evaluating the yeast extracts biostimulant properties using established methods in their laboratoryTask 9: -- Project Management: The principal investigator will hold a day-long kick off meeting at a retreat with staff and consultants as well as staff meetings weekly thereafter.

Progress 09/01/20 to 08/31/22

Outputs
Target Audience:Maine Shellfish Devdelopers LLC (MSD) envisions and has begun toimplement closed farming of oystersand other shellfish at non--coastal sitesanywhere. This is a long over duebteaking awayfrom the ecological and regulatorylimit that hold the industry back even as demand soars.Our goal is to frachise the method nation-wide much as if a network of family-owned dairies, except that ours will be located close to population centers. This will provide readily availabe fresh and traceable seafod throughout the US to our main target customers, the white tableclothrestaurants. . MSD will supp;y the franchisees withfeed and seed as well as marketing services much as a soft drink company provides its bottlig plants with syrurp while marketing the product(s),except thatt our "syrup" will be large volumes of cistost effectivemicroalgae.The latter was not possibleuntil MSDand a nearby collabrating institytuion, the Center for Algal Innovation, made atechnical breakthrough nine months following the compleion of this Phase I . The wotk was fundee by a NSF Phase I award that overlappped this USDA Phase II project by three months. Th franchisees will become, in effect, a secomd set of target customers. Microalgae has potentially many used other than feeding shellfisj. It has eitht times more proin by weight than rice and as much as soy but unluke rice, sotycorn and wheat, they microalgae have amp;e omega-3 fatty acids and much more. An ability to priduce icostly nutrientscheaply portends their production by large companiesfor human food and animal feed. It seem probable that MSD/CAI will from a joimtly owned licensing company early in 2025. Those large companies wil comprise our third set of yarget customers. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? MAJOR GOALs 1.AutOMATED PRODUCTION SYSTEM: Somelarge, ciccular fish tanks alreafy installed pre-pandemic were found to be unsuitablevfor shellfish purposaes. We saved the tanks for wasre water management purposesand chose insteat to eadapt vertival tankage (see Objective 2 below) such as employed by some hydroponic and cannabis gtowers but for purely quatic purposes.. This made posible an entirely different automated systemwhereby a robotic grab hanging from ceilimg tracks could unfer computerized control be directed to grab and transport stock-loadeed, web-bagsfrom open raceways, transporting these to the wrkplac for processing and packaging. Once there the grab will alow them oysters to beemptied into a hopper.From there the oysters are to be washed, tumbled, counted, and weighed with some packaged for market and oth the remainder re-bagged and reurned to for more grow-out. The bags or sets of bagswill cary chipswithfresh data. Since there wil be a maually operated protype production in Year-1 and the full completed systemnot until Q-4 of Year-2(May 2026), we anticipate that AI will have progresssed to where its inclusion for data management will be realstic. With 4-stacks and 12-raceways per Unit and, ultimately five Units per 20,000 sq,ft builfimg (20-stacks/60-raceways), there will be tens of thousands of oysters povessed hourly. Conciveably, eacj stack could have a somewhat different diet, flow rate, loadomg ca[acity, etc. AI coul weighed the relative benefits and not only recommend changes but imp;ememt the best. MSD 's AI could monitor the information collected by each franchisee in a natoon-wide net andwill allow an extraodinart lovel of biological self-imptovement,all monitored atMSD's headwuarters. 2. SYSTEM DESIGN FOR LARGE-SCALE EXPANSION:EX[AN: A single priduction Unit consists of twp cleansing tanks, four stacks of three 20' fiberglass raceways each (see Objective 2 below), and a compiterized processing an pavkagng Woekpla plis a robotic stockmanagement and control system. With a truncated Workplace, we term this our Low--Vo9loume Modelce. It is rated to yieldharvests of 20,000 oysters per week. On a 50-week yeat. Thi8s comes to one millioipnoysters from equipment occupyiing 60'x 60' (1.0M.OY/Year). In order to expand rapidly, MSD will francgise LVMs nation-wide not unlike a nerwork of family dairies exept that ours will be located close to population centers. We will recommend tha franchisees start with one LVM but in a buolding -- that might be a rentedd watehouse - with space enough to acomodate four more LVMS and an expanded Workspace. We term five LVMs to be one High-Volume Model (HVM), the sole difference is that HVMs will confer significant economies of scale thanks to sharing the system and the staff. SPECIFIC OBJECTIVES 1. ACHIEVE WATER QUALITY BETER THAN IN THE ESTUARIES: In fact, since we begin with man-made seawateeer and wil have in place a 100% recirculating capability,seawater at non-coastal sites will always exceed near-shore quality, this is achieved with a hybrid of installed of a shelfish-specificf chemo-mecanical system (SS-RAS) and conventional RAS . The former consists of multitrophic and aquqaa ponic treatments, one utilizina a well aerated tank of seaweed, the other by filteringout particulates to be utilized asa resuece to culture protozoansas acomp;ementary feed to microalgae (Subsequent to the completion of Phase II, MSD and colleagies at the nearbt Center for Algal Innovaion devised a means of uniquely culturing cost effective microalgae) -- see Target Sustomers_. 2. DESIGN A GANTRY-BASED STOCK TRANSGER SYSTEM:Much o:f Year-1 wa lost attempting to making an overhead gantry serve to halul out large wwire cyliners packed with oysters that were stood verticlly in the deep, circulat tanks. This was was ergonomically clumsy an, when transferring oysters to the Wwrkplacem un-safe, Instear, sraff opteted to adapt an approch employed by some Hhdroponic and Cannibis growers by stackiind three levels of 20' fiberglass raceways, .Amocj up was assembed, each 4' wde and 1' deepwith a cente divider so that the feed-loaded flow could reuclock-wise one day anf counter-clockwise the next, A single LM wiwill have four stacks with three levls each, hence 12- raceway thus one HVM 950M/Oy/Tear) would have 20-stacks and 60 raceways

Publications


    Progress 09/01/20 to 08/31/21

    Outputs
    Target Audience:The target audiences for the project overall are1) today's near-shore seedling suppliers such as Norumbega Oyster Farm 2) a local fish market such as Harbor Fish Market in Portland ME and 3) a seafood distributor in Boston. During this reporting period - Year 1 of the 2-Year project - near-shore seedling supplier Norumbega Oysterhas supplied seedlings for growout and testing and is in close contact with progress on the project. A local fish market and a seafood distributor in Boston will be reached during Year 2. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

    Impacts
    What was accomplished under these goals? Maine Shellfish Developers LLC is transforming shellfish farming to 100% onland closed-containment production - keeping them where they began life all the way to market-size. Year-round, environmentally insulated, and offering large-scale efficiencies, this can make a major contribution to sustainable national and global food security. Completing the first onland closed-containment operational oyster farm is a major goal of this project with a specific objective of achieving and maintaining better water quality for oysters than their natural environment. MSD has completed installation of the four tanks as well as all basic plumbing. Two of the four tanks have become fully operational and the remaining two tanks are being phased in during Year 2. An automated production system providing unprecedented levels of animal husbandry with minimal staffing requirement will lay the foundation for large scale low cost per unit oyster production. MSD has completed the design of an overhead beam/gantry operating system that can span the range from a semi-automated 4-tank version up to a highly automated 20-tank version and that can be implemented in stages. Details were included in MSD's Phase II Interim Technical Report submitted in September 2021. Designing the complete production system as a unit for modular large scale expansion is a vital element of rapid cost-effective scale up. MSD's existing 4-tank system has been constructed with that objective in mind. When all 4 tanks become fully operational and stabilized, detailed design of the finished modular unit will be completed and included in the plans and costs for Phase III expansion. A detailed plan for Phase III expansion will be include in the Phase II project Final Report. This will include costs and financing projections for near-term expansion into a proposed adjacent HVM showcase. Broad outlines of the plan are complete awaiting details that will be finalized during Year 2.

    Publications