Source: PENNSYLVANIA STATE UNIVERSITY submitted to
APPLICATION OF DIAGNOSTIC SERVICES TO SUSTAIN AND PROMOTE ONE HEALTH
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
NEW
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1023391
Grant No.
(N/A)
Project No.
PEN04748
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Jul 1, 2020
Project End Date
Jun 30, 2025
Grant Year
(N/A)
Project Director
Jayarao, BH.
Recipient Organization
PENNSYLVANIA STATE UNIVERSITY
208 MUELLER LABORATORY
UNIVERSITY PARK,PA 16802
Performing Department
Veterinary & Biomedical Sciences
Non Technical Summary
Agriculture is the No. 1 industry in Pennsylvania, and animal agriculture plays a major role. The sale of livestock and poultry products accounts for nearly 70% of Pennsylvania's farm income. To keep Pennsylvania's Animal Agriculture secure, Pennsylvania Department of Agriculture's Bureau of Animal Health and Diagnostics (BAHDS) oversees this critical function. This work is critical for protecting the US livestock and poultry industries from foreign animal diseases, which, in turn, protects our food supply and our citizens. A healthy animal agriculture industry also is a key part of ensuring that Pennsylvania's trade status with other states and other nations is not negatively impacted by an outbreak within our borders. BAHDS oversees the operations of the Pennsylvania Animal Diagnostic Laboratory System, or PADLS. The PADLS system connects the Pennsylvania Veterinary Laboratory (PVL) in Harrisburg, Penn State University's Animal Diagnostic Laboratory (ADL) and the University of Pennsylvania's School of Veterinary Medicine at New Bolton Center. The Animal Diagnostic Laboratory at Penn State is a critical entity in the network of veterinary epidemic intelligence and vital to the maintenance and well-being of a modern animal industry. Penn State ADL not only provides diagnostic services to animal industry in Pennsylvania but also performs cutting edge research to address diseases of animals and humans. Penn State ADL shares the responsibility for controlling and eradicating diseases in livestock and poultry. This mission is critical since these diseases have the potential to impact human health and cause significant economic loss to farmers in the Commonwealth.
Animal Health Component
100%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3113299104010%
3113299110010%
3113299110110%
3113299116015%
3113470104010%
3113470110010%
3113470110110%
3113470116015%
3113899116010%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3299 - Poultry, general/other; 3899 - Other animals, general; 3470 - Other dairy cattle products;

Field Of Science
1040 - Molecular biology; 1101 - Virology; 1160 - Pathology; 1100 - Bacteriology;
Goals / Objectives
In January 2020, Pennsylvania's 52,700 farm families continue to be the stewards of more than 7.3 million acres of farmland. Agriculture is the No. 1 industry in Pennsylvania, and animal agriculture plays a major role. The sale of livestock and poultry products accounts for nearly 70% of Pennsylvania's farm income. In 2019, Pennsylvania ranked 6th nationally in total milk production. The Commonwealth's 480,000 cows produce 10.1 billion pounds of milk. The dairy industry is the key economic driver in Pennsylvania's agriculture industry, the Commonwealth's leading economic enterprise. Pennsylvania ranks fourth nationally in trout production. The 2019 the estimated value of trout production in Pennsylvania was $14.8 million. In 2018, Pennsylvania equine owners allocated 793,000 acres of land for equine purposes with associated assets totaling $1.7 billion. Pennsylvania ranks fourth nationally in egg production and 10th nationally in turkey production. The poultry industry is the second largest animal agriculture in PA and the estimated value of this overall industry exceeds $1 billion. Pennsylvania is home to approximately 2,700 independent hog producers. The pork industry in Pennsylvania ranks 13th in the nation with $331.5 million in sales. The industry supports more than 8,000 jobs and generates around $67.5 million in export income while creating more than 1,600 export jobs.To secure and keep Pennsylvania's Animal Agriculture vibrant and thriving in Pennsylvania, the Pennsylvania Department of Agriculture's Bureau of Animal Health and Diagnostics (BAHDS) is indispensable to animal agriculture in Pennsylvania. With millions of livestock and poultry in Pennsylvania, BAHDS has a presence across the state. This work is critical for protecting the US livestock and poultry industries from foreign animal diseases, which, in turn, protects our food supply and our citizens. A healthy animal agriculture industry also is a key part of ensuring that Pennsylvania's trade status with other states and other nations is not negatively impacted by an outbreak within our borders. BAHDS administers regulatory programs for animal health certification, containment of diseased animals and elimination of disease agents. BAHDS oversees the operations of the Pennsylvania Animal Diagnostic Laboratory System, or PADLS. The PADLS system connects the Pennsylvania Veterinary Laboratory (PVL) in Harrisburg, Penn State University's Animal Diagnostic Laboratory (ADL) and the University of Pennsylvania's School of Veterinary Medicine at New Bolton Center.The Animal Diagnostic Laboratory at Penn State is a critical entity in the network of veterinary epidemic intelligence and vital to the maintenance and well-being of a modern animal industry. Penn State ADL not only provides diagnostic services to animal industry in Pennsylvania but also performs cutting edge research to address diseases of animals and humans. Much of the research work that is done on animal diseases has a direct impact on human disease research. Penn State ADL shares the responsibility for controlling and eradicating diseases in livestock and poultry. This mission is critical since these diseases have the potential to impact human health and cause significant economic loss to farmers in the Commonwealth.1) To detect, diagnose and conduct surveillance for diseases of mammalian and avian species in the State of Pennsylvania (Jayarao, Kuchipudi, Dunn, Wallner-Pendleton, Lighty, Brooks, Luley, Surendran Nair, Lu)2) To develop and validate diagnostic tests to detect and diagnose diseases of mammalian and avian species (Jayarao, Kuchipudi, Lighty, Brooks, Luley, Surendran Nair, Lu)3) To conduct basic and translational research on diseases of economic and zoonotic importance to animal and human health (Jayarao, Kuchipudi, Lighty, Brooks, Luley, Surendran Nair, Lu)4) To train and mentor veterinarians, residents, visiting scholars, post-doctoral fellows, and undergraduate and graduate students(Jayarao, Kuchipudi, Dunn, Wallner-Pendleton, Lighty, Brooks, Luley, Surendran Nair, Lu)
Project Methods
The Animal Diagnostic Laboratory (ADL) at The Pennsylvania State University is a full service AAVLD accredited veterinary diagnostic laboratory. Standard operating procedures are used to perform and report diagnostic tests and their results to the clientele. Diagnostic tests that will be developed will be validated by the Scientific Discipline Group of PADLS and approved by the Animal Health and Diagnostic Commission before they can be used in the laboratory. Disease surveillance will be performed based on the protocols and procedures provided by the state or the federal agencies. Research projects will be done on approval of Penn State IRBand IUCAC.Over a period of five years, thisproject will perform and offer diagnostic services, conduct surveillance, conduct research on animal and human diseases, train students and professionals, disseminate research findings and develop outreach materials.

Progress 07/01/20 to 09/30/20

Outputs
Target Audience:Veterinarians, Dairy and beef producers, Poultry producers (all species and types, commercial poultry, backyard producers, organic producers, game bird producers, live bird market producers), Other clientele (deer, elk, swine, sheep, goat, bird fanciers, horses, wildlife rehabilitators). Researchers, Federal Govt. Institutions and Regulatory Agencies (USDA-Animal and Plant Health Inspection Service, USDA-National Animal Health Laboratory Network, USDA-National Veterinary Services Laboratories), State and Local Law Enforcement Agencies, Pennsylvania Department of Agriculture, Bureau of Animal Health and Diagnostic Services, Animal Health and Diagnostic Commission, Pennsylvania Game Commission, pharmaceutical and biologics companies, welfare organizations, American Association of Veterinary Laboratory Diagnosticians, American Board of Toxicology, Penn Ag Industries Association, Pennsylvania Association of Sustainable Agriculture, undergraduate and graduate students, research scientists, and general public. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?USDA NIFA Practitioners' meeting (virtual). Presentations on poultry internal and external parasites, as well as Staph aureus infection (July 24, 2020). CL Davis Foundation Webinar. Topics in Forensic Pathology. Estimating the postmortem interval, pathology of gunshot wounds. September 8, 2020. In addition, this research provides training and mentoring opportunities for the following (Goal 4): Visiting scholar (1) mentored by Suresh Kuchipudi on molecular and genetic characterization of rabies viruses in India Graduate student (1) mentored by Suresh Kuchipudi on molecular mechanisms of Zika virus pathogenesis Resident in Microbiology (1) mentored by Suresh Kuchipudi on clinical veterinary microbiology and methods for detection of veterinary pathogens Undergraduate students (4) mentored by Suresh Kuchipudi on investigation of the molecular mechanism of Virus host interactions Post-doctoral scientist (1) mentored by Suresh Kuchipudi onsusceptibility and potential adaptation of SARS-CoV2 to livestock Resident in Avian Medicine (1) mentored by Eva Wallner Pendleton, Megan Lighty, and Patty Dunn on avian medicine and pathology Veterinarians (60) mentored by Eva Wallner Pendleton during Poultry Medicine Workshop Veterinarians (2) mentored by Eva Wallner Pendleton on diseases of captive gamebirds Undergraduate students (3) mentored by Huaguang Lu on diagnostic virology Research Fellows (2) mentored by Huaguang Lu participating in the Cooperative Threat Reduction Biosciences Fellowship Program, (CRDF Global Fellowship), and Avian Reovirus Transmission studies Visiting Scholar (1) mentored by Megan Lighty and Patty Dunnonavian medicine and pathology Undergraduate Student (1) mentored by Jason Brooks in veterinary forensic science Resident in Pathology (1) mentored by Jason Brooks onmammalian pathology How have the results been disseminated to communities of interest?Results have been disseminated to communities of interest through conference presentations, peer reviewed journal articles, a book chapter, and the Penn State Extension website. What do you plan to do during the next reporting period to accomplish the goals?1) Provide Diagnostic Testing Services (Goal 2) 2) Perform Research on Reoviruses, Avian Influenza viruses, and Bovine Herpes Virus (Goal 3) 3) Undertake Field Investigation 4) Meet with Clientele and address their questions and issues 5) Apply for research grants 6) Prepare for AAVLD site visit 8) Provide externships for veterinary students, undergraduate and graduate students (Goal 4) 9) Consult with vendors 10) Provide technical consultation on animal diseases to drug industry

Impacts
What was accomplished under these goals? During the reporting period, October 1, 2019-September 30, 2020, 7743 cases were handled by ADL for diagnostic purposes accounting for 200,915 specimens (Goal 1). The specimens were from avian species (including wildlife), dairy and beef cattle, wild mammals, domestic animals, deer, and fish. The mammalian and avian field investigation group performed 137 and 59 field investigations, respectively.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Nissly RH, Zaman N, Ibrahim PAS, McDaniel K, Lim L, Kiser JN, Bird I, Chothe SK, Bhushan GL, Vandegrift K, Neibergs HL, Kuchipudi SV. Influenza C and D viral load in cattle correlates with bovine respiratory disease (BRD): Emerging role of orthomyxoviruses in the pathogenesis of BRD. Virology. 2020 Sep 26;551:10-15. doi: 10.1016/j.virol.2020.08.014. Epub ahead of print. PMID: 33010670
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Salazar E, Kuchipudi SV, Christensen PA, Eagar T, Yi X, Zhao P, Jin Z, Long SW, Olsen RJ, Chen J, Castillo B, Leveque C, Towers D, Lavinder JJ, Gollihar J, Cardona JA, Ippolito GC, Nissly RH, Bird I, Greenawalt D, Rossi RM, Gontu A, Srinivasan S, Poojary I, Cattadori IM, Hudson P, Josleyn NM, Prugar L, Huie KE, Herbert AS, Bernard DW, Dye JM, Kapur V, Musser JM. Convalescent plasma anti-SARS-CoV-2 spike protein ectodomain and receptor binding domain IgG correlate with virus neutralization. J Clin Invest. 2020 Sep 10:141206. doi: 10.1172/JCI141206. Epub ahead of print. PMID: 32910806.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Byukusenge M, Nissly RH, Li L, Pierre T, Mathews T, Wallner-Pendleton E, Dunn P, Barnhart D, Loughrey S, Davison S, Kelly DJ, Tewari D, Jayarao BM, Kuchipudi SV. Complete Genome Sequences of Seven Avibacterium Paragallinarum Isolates from Poultry Farms in Pennsylvania, USA. Microbiol Resour Announc. 2020 Jul 2;9(27):e00654-20. doi: 10.1128/MRA.00654-20. PMID: 32616650; PMCID: PMC7330252.
  • Type: Websites Status: Published Year Published: 2020 Citation: M. Lighty. Deworming Backyard Poultry. In: Penn State Poultry Extension Website. Modified July 2020. https://extension.psu.edu/deworming-backyard-poultry
  • Type: Book Chapters Status: Published Year Published: 2020 Citation: Brooks JW. The Forensic Necropsy. In: Byrd, Norris, & Bradley, eds. Veterinary Forensic Medicine and Forensic Sciences. Boca Raton, FL: CRC Press. 2020.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Lighty, M. 2020. Oral Presentation: Nature of Disease: Zoonoses and Pandemics. Northeastern Conference on Avian Diseases. September 15. Virtual Meeting.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Lighty, M. 2020. Oral Presentation: Poultry Health and Disease. 4-H Poultry Project Series. July 16. Virtual Meeting.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Lighty, M. 2020 Oral Presentation: Maximizing the Value of Your Diagnostic Lab Submissions. Pennsylvania Poultry Sales and Service Conference. September 16. Virtual Meeting
  • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Lighty, M. 2020. Oral Presentation: Viral Hepatitis in Turkey Poults. American Association of Avian Pathologists Scientific Program. August 4, Virtual Meeting