Performing Department
(N/A)
Non Technical Summary
The problem of losses due to insect and microbiological pests does not end in the field or with the harvest. The problem persists into the storage phase where the value of raw commodities is reduced by direct damage. Thus sensing and eliminating insect pests must be accomplished to ensure not only the retention of maximum value of the crop but also to permit the safe movement of agricultural commodities from infested and/or infected areas to those that are not. In addition, stored grains like corn, wheat, and rice as well as nuts and fruits and tobacco are processed into value-added products that are susceptible to insect attack. Traditionally, post harvest pest control has been achieved predominantly through use of methyl bromide and other fumigants. The strategic use of post-harvest fumigants is required due to human and environmental health regulations. If insect infestations can be sensed before the onset of damage, via the technologies and cooperation in this project, a reduction in the amount of fumigant used for disinfestation, a reduction in the frequency of fumigation, and an increase in fumigation efficiency is expected.This project focuses on the Indianmeal moth and its larvae. The Indianmeal moth is the most common stored product insect found throughout the U.S. Adult Indianmeal moths can be found almost anywhere in the temperate regions of the world. In the U.S. and Europe it is the one insect pest that causes the most damage. The economic losses from these pests in processing, transporting and storing can be in the millions of dollars per contamination incident, product recall, consumer complaint/litigation, and pest control applications. Yet there is no efficient, low cost method to monitor and sense Indianmeal moths.The Indianmeal moth is most often found feeding on finished food products, the ingredients for food such as stored wheat products, milled/processed wheat, and other stored products such as milled cereal products, flour, bran, pasta products, spices or infesting equipment where food is prepared, processed, packaged or stored. Indianmeal moth larvae are the destructive life stage of the insect, eating voraciously. The larvae are highly mobile and continuously seek out new sources of food. While there are no direct health concerns, the psychological health of an individual knowingly consuming these larvae can have dramatic impacts and consequences including legal costs for the retailer, distributor or manufacturer of the product. It is the oneinsect found more often than any other on stored food and grain in the U.S. Over fifty years the genetics of this insect have changed to resist the commonly used pesticide Malathion. In the 1970's, the Indianmeal moth started showing signs of resistance to this insecticide. Since then, the Indianmeal moth has become the most resistant insect known to man. As the other insect competitors for the same habitat and food sources were slower to develop this genetic resistance to Malathion, the Indianmeal moth emerged as the primary pest of stored products.SDC's objective in this project is to develop an affordable and accurate device that can detect Indianmeal moth and Indianmeal moth larvae in stored products. No other technology has been shown to sense larvae in stored products. SDC's technology is pioneering. The SDC device will provide early warning of insects in food processing and storage to help prevent infestation and control populations and drastically reduce the need for hazardous chemicals in the food industry. The primary goal of this project is to implement SDC's innovative nano-tin oxide sensor technology to sense pheromones and semiochemicals, chemicals given off by insects to communicate with other insects. SDC will sense these chemicals simultaneously at very low levels and to differentiate between them and other gases in the air. SDC anticipates that if the project is successful the most damaging insect of stored products will be sensed, and the size of their populations reported. The key technical benefit is a better quality reading of insect and larva populations. This informs the pest manager of the best time to fumigate and prevents overuse of chemical fumigants. Today's usual procedure, trapping and counting insects in a discrete sample, then projecting a population for the total is subject to sampling error and could result in unnecessary fumigation.SDC's platform technology is based on the ability of its sensors to sense virtually any reducing gas with extremely high sensitivity, so the potential for follow-on applications is significant. Using this technology platform, SDC has plans to develop additional pheromone-based sensing devices for other insects in the agricultural grain storage and stored products markets. For example, SDC's success in sensing the warehouse beetle could be extended to sensing the Khapra beetle, which is subject to quarantine, because the Khapra beetle has the same pheromone as the warehouse beetle. Additionally, SDC recognizes the opportunities available to it and its potential corporate collaborators to use this technology for applications beyond agriculture such as medical diagnosis of pulmonary fungal infections, odor detection, monitoring indoor air quality, mold detection in residences and hospitals, and homeland security. It may be especially useful in this context for the federal government, which could use the device in forestry applications to locate termites that attack and destroy forests and forest products. Bed bug incidences are on the rise across the country, and there may be a need in the future for the government's application of SDC's technology to combat this pest. Federal buildings in Cleveland, Ohio, Washington D.C. and St. Albans, Vermont have had bed bug incursions. Finally, the federal government could use this highly sensitive, portable device to scan for explosives.
Animal Health Component
50%
Research Effort Categories
Basic
(N/A)
Applied
50%
Developmental
50%
Goals / Objectives
This project's overarching goal is to retain maximum value of agricultural commodities and to permit their safe movement from infested and/or infected areas to those that are not while avoiding overuse of fumigants and insecticides.The problem of losses due to insect and microbiological pests does not end in the field or with the harvest but persists into the storage phase where the value of commodities is reduced by direct pest damage. Strategic use of post-harvest fumigants is required due to human and environmental health regulations. Postharvest pest control has been achieved predominantly through use of methyl bromide and other fumigants.Detecting insect infestations before the onset of damage, via the technologies and cooperation described below, can increase the efficiency of fumigation and reduce the amount of fumigant used for disinfestation, as well as the fumigation frequency. This can be achieved by sensing and eliminating insect pests. In addition, stored grains like corn, wheat, and rice as well as nuts and fruits are processed into value-added products that are susceptible to insect attack.Sensor Development Corporation's (SDC's) specific goal in this project is to develop an affordable sensor that enables pest sensing in an extensive number of venues where economics and health issues occur. This single portable unit will sense the Indianmeal moth (IMM) adult insects, larvae and cocoons below the targeted infestation number, allowing pest managers to take action to limit damage from these pests and contain them to specific areas early. This is done by sensing the Indianmeal moth adult female's sex pheromones and by sensing the semiochemical kairomones in Indianmeal moth larval frass and mandibular gland secretions. The Indianmeal moth is the most common stored product insect found throughout the U.S. It is the one insect found more often than any other on stored food and grain in the U.S. The adult Indianmeal moth can be found almost anywhere in the temperate regions of the world. In the U.S. and Europe it is the one insect pest that causes the most damage.The technical objectives of this Phase II proposal are organized and assigned according to the expertise and the resources of the four project collaborators: San Joaquin Valley Agricultural Sciences Center USDA ARS, Parlier, CA (San Joaquin Center), The Richard Desich SMART Commercialization Center for Microsystems (Elyria, OH), Local Electronics Design House, and Sensor Development Corporation (Elyria, OH) under PI N.J. Smilanich. The ultimate goal, which has promise based on SDC's preliminary catalyst work, is to achieve selective and sensitive sensing of IMM adult female's pheromone and of IMM larval and cocoon semiochemical kairomones with chips customized for the pheromone and for the semiochemical. The Phase II objectives and descriptions of expected results in each objective follow. Participation by the San Joaquin Center, SDC's insect and pheromone expert collaborators and Local Electronics Design House, SDC's electronics supplier, is indicated as appropriate.SDC will accomplish its goal with the following three Objectives:Technical Objective 1. (SDC, SMART Commercialization Center, Local Electronics Design House) Revise the pre-prototype design to a commercial product design with all features of the commercial productThe environment targeted for our insect sensor demands the device and the interconnections to be able to withstand shock and drop accompanying use in an uncontrolled user environment, not unlike shake/drop conditions to which cell phones are subjected. One important task in this objective is to work with an electronic component designer to create a more rugged design. The scope of the work proposed in this task includes developing improved chip-carrier connections, as well as more robust carrier-holder connections. The objective is to make the connections able to survive: 1) sinusoidal and random vibration; and 2) shock caused by a drop from roughly countertop height.The approach for this work is to propose a design that meets SDC's technical and price point objectives. After this design is completed, reviewed and approved, a prototype first sample commercial product will be built. The prototype first sample's performance will be judged against established performance criteria for the commercial product. Any gross errors/problems in the proposed design will be corrected.Technical Objective 2. (SDC, San Joaquin Center, Local Electronics Design House) Build, prove-in and field test a first sample commercial product based on the revised design.Technical Objective 2 builds the new design using replacement components and checks fit, function, and design for manufacturability. Prove-in tests are run at the lab bench to show that the new design meets all commercial product performance criteria. After this demo, a field trial will be run with live adult female IMM and with larvae and larvae in cocoons. This field trial will show that the first commercial sample product meets the commercial product performance criteria in the less controlled conditions of a field trial.Technical Objective 3. (SDC, Selected Customers) Conduct beta-testing of the first commercial sample to advance the demonstration that this beta-version fulfills all expected commercial product performance criteria.Technical Objective 3 interfaces SDC's technical program with its commercialization program. A successful demo of the first commercial sample's performance forms the basis for building 10 more beta version commercial samples using external funding. Beta Test and Purchase Agreements will be executed with selected prospective customers. Trials will be run in storage facilities or warehouses of food ingredients or finished food products made or used by these customers. SDC expects that these selected customers may suggest changes to the product after testing. These changes will be considered and the product modified as warranted prior to official product launch.The deliverables from completing these Phase II objectives build seamlessly on the achievements of the completed Phase I project and lead on to commercialization.SDC's Technical Objectives described above complement the Specific Aims of the work that will be done by the San Joaquin Center in the CRADA agreed by SDC and the San Joaquin Center.The goals of the CRADA work to be done at the San Joaquin Center are to enhance the competitiveness of US agriculture via development of efficient,economical, and environmentally responsible strategies to overcome post-harvest losses from insect infestation, thereby ensuring the retention and expansion of U.S. grown products in the global marketplace.The research goal is to develop novel sensor technologies for identifying infestations of Indianmeal moth in stores of dried fruit and tree nuts.The research will involve a multipronged approach and is outlined here:Specific Aim 1: Isolate and characterize 2-acyl-1,3-cyclohexanediones from larval mandibular glands and/or feces of the Indianmeal moth, Plodia interpunctella, and almond moth, Cadra cautella.Specific Aim 2: Synthesize gram-scale quantities of natural products from Aim1 and confirm characterization.Specific Aim 3: Using synthetic material (Specific Aim 2): evaluate the fundamental physicochemical properties, including but not limited to Henry's Law solid to air partitioning distributions.Specific Aim 4: Verify that natural products (Aim 1) are naturally emitted from stored products infested with Indianmeal moth, Plodia interpunctella, and almond moth, Cadra cautella. Quantify rates of emission using a volatile collection apparatus.Specific Aim 5: Integrate results from Aims 1 through 4, and supply synthetic material to aid in the development of sensors diagnostic of Indianmeal moth, Plodia interpunctella, and almond moth, Cadra cautella,infestations.
Project Methods
SDC's methodologies specialize in engineering and commercial application of gas and volatile organic compound sensor technologies. SDC has expertise in high sensitivity volatile organic compound sensing as this technology relates to pest control in commercial as well as quarantine settings across the globe. For example, the warehouse beetle has the same pheromone as the Khapra beetle, which is subject to quarantine.SDC produces nano-crystalline tin oxide particles by sol-gel methods, fabricates sensor chips by microfabrication and thick-film techniques, assesses the semiconductor film properties by optical and analytical methods, and evaluates sensor performance using a custom computerized testing apparatus. SDC has developed methods for making sol-gels containing any one of a number of catalysts. These catalysts enhance the chip's response to specific gases, enabling selective detection of gas markers of different bio processes. This technology platform has been demonstrated as a way to sense VOC markers for molds and insects in stored grain, and for fungal infections in human lungs. Also, SDC designed and built a prototype including an electronics board, microprocessor, and the programming required to control the individual chips and collect the chip response in a digital text file ready for analysis. Six operating channels can be run at temperatures from 200°C to 400°C. This prototype forms the basis for many different products by redesigning the chips to suit particular VOCs and by making associated software changes.