Recipient Organization
YAUPON WELLNESS COMPANY, INC
1101 CHATHAM PKWY, UNIT E4
SAVANNAH,GA 31408
Performing Department
(N/A)
Non Technical Summary
Project Summary/AbstractWith over a trillion cups of caffeinated beverages consumed each year, caffeine is arguably the world's most frequently consumed stimulant. Caffeine is widely used to enhance athletic performance, body composition, pain relief, and mental acuity. Naturally caffeinated beverages include coffee, black and green tea, cola, cocoa, guarana, yerba maté, and Yaupon tea. According to the Tea Association of the USA, tea is the most widely consumed beverage in the world next to water. Tea can be found in 80 percent of American households. Of the caffeine sources listed above, the wild yaupon growing in the American southeast is the only significant native source of caffeine in the continental US. Yaupon tea was a significant part of Native American and early American culture and is undergoing resurgence in popularity in both the U.S. and E.U. with raw material for processing coming from wild collected sources. Yaupon Holly is widely cultivated for its ornamental value however, there are no known Yaupon Holly plantations established for production as a food. The Yaupon Wellness Company (YWC), is a rural Georgia based company founded in 2013, that wild-picks, dries, roasts, brews, and distributes certified organic bottled Yaupon tea and dry ASI tea products. YWC is currently harvesting approximately 500 pounds of wild Georgia Yaupon leaves per month. The company has developed distribution channels and has launched the bottling of its Yaupon Tea for the Ready-to-Drink (RTD) market.The current barrier facing YWC's commercial viability is the need for a reliable, sustainable source of yaupon holly. Cultivation of Yaupon for food purposes offers a new crop opportunity for quality minded small and mid-sized farms therefore the PI has established the YWC Pilot Farm in order to begin development of optimal production practices and serve as a model for other growers to replicate. This project proposes to utilize this initial planting to evaluate the technical and financial feasibility of cultivating Yaupon Holly for it food value. Specifically the project will: 1) Characterize clonal plantings of Yaupon on the YWC Pilot Farm from chemical and agronomic perspective. 2)Compile plantation establishment and production costs together with yields through one production cycle of the pilot plantation. 3)Utilize the above data to develop a preliminary enterprise budget for Yaupon production based on actual establishment and production costs. 4) Begin development of an outreach plan for recruiting and training potential growers.Successful achievement of these objectives will result in the development of a baseline dataset of Yaupon properties that will be used to evaluate the effects of treatments and make a preliminary determination of financial feasibility. In addition, an outreach plan focused on small farmers will be developed with the assistance of extension and small farm specialists. Assuming a successful outcome to Phase I, Phase II will expand production research and optimize the production system through at least two additional cycles. Phase II will also evaluate the feasibility of an on-farm processing facility and fully develop outreach and training programs including a plan for mentoring new growers through their first production cycle. Phase III will commercialize the research by partnering with existing small farm outreach efforts to present the plan to growers with the goal of establishing 100 acres of Yaupon food production during the first three years.Benefits from a successful project include enhanced profitability and farm diversity for growers, a higher quality, more reliable source of Yaupon raw material for processors, enhanced consumer safety and satisfaction, and positive environmental effects through a reduction in the need to wild collect Yaupon to meet increasing market demand.
Animal Health Component
33%
Research Effort Categories
Basic
34%
Applied
33%
Developmental
33%
Goals / Objectives
In order to begin development of the needed data, the PI has established a pilot Yaupon plantation consisting of 10,000 plants across approximately 12 acres. The work plan of this Phase I SBIR proposal will utilize this planting plus expertise from Clemson University to determine the effect of shade and fertilization and evaluate the technical and financial feasibility of commercial production of Yaupon for its food value. Specifically, the project team will:1. Characterize clonal plantings of Yaupon on the YWC Pilot Farm from chemical and agronomic perspective.2. Compile plantation establishment and production costs together with yields through one production cycle of the pilot plantation.3. Utilize the above data to develop a preliminary enterprise budget for Yaupon production based on actual establishment and production costs.4. Begin development of an outreach plan for recruiting and training potential growers.}Successful achievement of these objectives will result in the development of a baseline dataset of Yaupon properties that will be used to evaluate the effects of future treatments and make a preliminary determination of financial feasibility. In addition, an outreach plan focused on small farmers will be developed with the assistance of extension and small farm specialists.Technical feasibility will be deemed to have been demonstrated when the baseline dataset has been established and data collected from one production cycle of the pilot plantation. Financial feasibility of the project will be deemed to have been demonstrated if the enterprise budget projections indicate a minimum farm gate price of $8 per dry pound will produce a gross income of at least $5,000 per acre by the fourth year after establishment.Assuming a successful outcome to Phase I, Phase II will replicate the research through at least two additional cycles providing multi-year data and conduct taste testing to determine the effect of the treatments on the final product. Phase II will also develop Good Agricultural Practices (GAP) compliant post-harvest handling and processing protocols, evaluate the feasibility of an on-farm processing facility, and fully develop outreach and training programs as well as a plan for mentoring new growers through their first production cycle. Phase III will commercialize the research by partnering with existing small farm outreach efforts to present the plan to potential growers with the goal of establishing 100 acres of Yaupon food production during the first three years of Phase III.}Benefits from a successful project include enhanced profitability and farm diversity for growers, a higher quality, more reliable source of Yaupon raw material for processors, enhanced consumer safety and satisfaction, and positive environmental effects through a reduction in the need to wild collect Yaupon to meet increasing market demand. Technical ObjectivesThe technical objectives and questions to be answered by this research are:1. Characterize clonal plantings of Yaupon on the YWC Pilot Farm from chemical and agronomic perspective.How are yield and chemical markers (Caffeine, Theobromine, and anti-oxidant potential {Hydrophillic Oxygen Radical Absorbance Capacity- ORAC}) affected by varying combinations of shade and fertilization?Does the plant form vary from shade and fertilization to facilitate or antagonize mechanical harvesting?Do desirable characteristics vary with harvest date?Successfully answering these questions will provide a preliminary basis for optimal cultivation of yaupon.2. Compile plantation establishment and production costs together with yields through one production cycle of the pilot plantation.What are the costs associated with establishing and producing Yaupon for food production?3. Utilize the above data to develop a preliminary enterprise budget for Yaupon production based on actual establishment and production costs.What is the total cost per acre to establish and maintain a Yaupon plantation for food purposes?How much income can a grower expect from one acre of Yaupon?Is the income competitive with other crops at the anticipated price level?Is it feasible for processors to pay the prices required for grower profitability?Successfully answering these questions will determine financial feasibility.4. Begin development of an outreach plan for recruiting and training potential growers.What is the best way to reach potential growers?Can we leverage existing small farm outreach programs in surrounding States?How can we effectively train new growers?}
Project Methods
Methods & Work Plan1. Characterize clonal plantings of Yaupon on the YWC Pilot Farm from chemical and agronomic perspective.Agronomic Characterization In Spring 2015, the PI established a Yaupon plantation on his farm located in Metter, GA consisting of 10,000 plants in rows 10 feet apart with 5 feet between plants for a total of approximately 12 acres.These plants were propagated by Innova Farms nursery in Boston, GA and delivered to the YWC farm in 5 gallon containers. The YWC Pilot Farm is currently transitioning to USDA National Organic Program certification.}?Upon notification of acceptance, Clemson University investigators will meet with members of Yaupon Wellness Company at the plantation in Savannah GA. An automated weather station with data capture on the farm will record temperature, humidity, rainfall and light. Temperature and light under the shade will be measured using handheld devices, comparing to the weather station in open field situation. Field plots will be laid out according to the experimental plan design below (see Figure 1 next page).Three nutrient applications rates with each level supplied by 4 rows of fertigation.Three shade levels (60%, 30% and 0), provided by two shade houses and an open space for 0, will run across the fertilizer treatments.Three harvest dates (May 31, July 15 and Aug 31) in the 2018 growing season.The completely randomized design, will be a full factorial, with repeated measures on each harvest date.Treatment 1. FertilizationTwelverows in each block will be fertigated using a Dosatron injection pump every two weeks withNeptune's Harvest Organic Hydrolyzed Fish Fertilizer (2-4-1). Emitters delivering 0.5 gallons per hour have been placed at each plant. The system will be activated for 1.5 hours per treatment resulting 0.75 gallons being delivered to each plant per treatment. Rows 1-4 will receive three ounces per gallon, rows 5-8 will receive one ounce per gallon and rows 9-12 will receive two ounces per gallon.Treatment 2. Shade30% shade will be constructed across the first 20 plants in all 12 rows. 60% shade will be constructed across plants 41-60. In rows 2,3; 6,7; and 10,11; plants 5-15; 25-35; and 45-55, will become the experimental plants. Therefore 20 interior plants, in all treatment factor blocks, will become the experimental units. The 20 sampled plants for each treatment block will be divided into 5 subplots of 4 plants each. Plant number 3 in each subplot is pre-selected, with 5 sentinel shoots on each shrub tagged for measuring shoot morphology. The tag will be placed 3 nodes before the terminal bud.On the harvest date, Clemson University and YWC Company staff will walk the experimental plots, making visual observation. Extension personnel will scout pests, diseases, or nutrient anomalies in the areas shown in Figure 1. The tagged sentinel shoots on the pre-selected shrubs will be severed 3 nodes above the tag removing new growth for that harvest period. Length of new growth, number of leaves, length of longest leaf on branch, leaf area (using Image J, https://imagej.nih.gov/ij/index.html) and thickness of longest leaf, will be measured and recorded for each sentinel shoot. The 5 shrubs in each subplot will be harvested by YWC Company farm crew with instruction of Clemson University personnel. Young shoots from each subplot will be placed in a paper sack Leaf fresh mass per subplot will measured and recorded. The sack will be slit open and contents laid flat, with 5 samples pulled from five precincts of the flattened shoot mass.The sampled shoots of each subplot will be separated into leaves and stems (a minimum of 20 g each sample) with the fresh weight recorded. Each sample will be assigned a unique identifier which will indicate the location from which the sample was taken. Samples from the even numbered subplots will be dried for 48 h at 40 C in paper envelopes. Dry mass will be determined and the harvested dry mass of each subplot will be estimated and recorded based on the % dry mass of the sample. These tasks will be performed by YWC employees and farm personnel at YWC Farm under supervision from Clemson personnel during the timeframe outlined above.All samples will be appropriately packaged and forwarded to Clemson University for chemical analysis and anti-oxidant potential testing. A complete set of tissue samples will also be kept at the YWC farm. Once the samples are received at Clemson, a hot water extract will be prepared from each dry samples for biochemical analysis. Two grams of dry leaf will be powdered using a ball mill. The powder will be steeped in 100 ml of boiled water held at a 100C for six minutes. The decoction will be centrifuged and the supernatant will be filtered through a 0.45µm nylon filter, and stored at -20C until analysis. RP-HPLC ProcedureCaffeine and theobromine concentration in the hot water extract will be quantified using Ultra High Pressure Liquid Chromatography (UHPLC) coupled to a Diode Array Detector. The compounds will be separated on a reverse phase column (Kinetic C18 XB, 3.0 x 150 mm, 2.6-μm particle size) using acetonitrile and 0.1% formic acid as the solvents. The compounds will be detected based on the absorbance at 274 nm, and the quantification will be done using the external standard curve prepared from commercial standards. Tentative identification of the separated components will be made by matching UV-Vis spectra and retention time match with commercial standards. Each sample will be analyzed in duplicates. The identity of the compounds in a set of subsamples will be further confirmed using an ultra-high resolution mass spectrometer (Orbitrap Fusion; Thermo Scientific) with an electrospray ionization interface following the above UHPLC condition. The identification will be based on accurate mass and fragmentation pattern of the compound/peak of interest.Hydrophilic-Oxygen Radical Absorbance Capacity (ORACFL) AssayA hydrophilic-ORACFL assay will be performed on the samples according to Robbins, Gong, Wells, Greenspan and Pegg (2015). A phosphate buffer (0.075 M, pH 7.4) will be used as the blank and diluent. Fluorescein (0.1 μM) will be employed as the reaction probe and 2,2′-azobis(2-amidino-propane) dihydrochloride, (AAPH, 80 μM in phosphate buffer), will be used as the radical initiator. Both working solutions will be held at 37 °C for the duration of the experiment. The phenolic extract will be diluted to 0.5 mg/mL with 95% (v/v) ethanol. The ethanolic solution will be further diluted with the phosphate buffer to a final concentration of 0.025 mg/mL. A standard curve based on five different Trolox concentrations (12.5, 25, 50, 80, and 100 μM in the phosphate buffer) will be constructed. The area under the kinetic curve (AUC) will be determined and following blank correction, samples and standards (i.e., 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid {Trolox}) will be compared. Final values will be reported as mmol Trolox eq./100-g sample from triplicate measurements.It is anticipated 108 assays will be required, 3 shade levels X 3 fertilization levels X 3 harvest dates for both stems and leaves (2), with 2 subplots in each treatment factor combination.