Source: YAUPON WELLNESS COMPANY, INC submitted to NRP
EVALUATING THE FEASIBILITY OF CULTIVATING YAUPON HOLLY FOR ITS FOOD VALUE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1015892
Grant No.
2018-33610-28278
Cumulative Award Amt.
$99,544.00
Proposal No.
2018-00189
Multistate No.
(N/A)
Project Start Date
Jul 1, 2018
Project End Date
Feb 28, 2019
Grant Year
2018
Program Code
[8.12]- Small and Mid-Size Farms
Recipient Organization
YAUPON WELLNESS COMPANY, INC
1101 CHATHAM PKWY, UNIT E4
SAVANNAH,GA 31408
Performing Department
(N/A)
Non Technical Summary
Project Summary/AbstractWith over a trillion cups of caffeinated beverages consumed each year, caffeine is arguably the world's most frequently consumed stimulant. Caffeine is widely used to enhance athletic performance, body composition, pain relief, and mental acuity. Naturally caffeinated beverages include coffee, black and green tea, cola, cocoa, guarana, yerba maté, and Yaupon tea. According to the Tea Association of the USA, tea is the most widely consumed beverage in the world next to water. Tea can be found in 80 percent of American households. Of the caffeine sources listed above, the wild yaupon growing in the American southeast is the only significant native source of caffeine in the continental US. Yaupon tea was a significant part of Native American and early American culture and is undergoing resurgence in popularity in both the U.S. and E.U. with raw material for processing coming from wild collected sources. Yaupon Holly is widely cultivated for its ornamental value however, there are no known Yaupon Holly plantations established for production as a food. The Yaupon Wellness Company (YWC), is a rural Georgia based company founded in 2013, that wild-picks, dries, roasts, brews, and distributes certified organic bottled Yaupon tea and dry ASI tea products. YWC is currently harvesting approximately 500 pounds of wild Georgia Yaupon leaves per month. The company has developed distribution channels and has launched the bottling of its Yaupon Tea for the Ready-to-Drink (RTD) market.The current barrier facing YWC's commercial viability is the need for a reliable, sustainable source of yaupon holly. Cultivation of Yaupon for food purposes offers a new crop opportunity for quality minded small and mid-sized farms therefore the PI has established the YWC Pilot Farm in order to begin development of optimal production practices and serve as a model for other growers to replicate. This project proposes to utilize this initial planting to evaluate the technical and financial feasibility of cultivating Yaupon Holly for it food value. Specifically the project will: 1) Characterize clonal plantings of Yaupon on the YWC Pilot Farm from chemical and agronomic perspective. 2)Compile plantation establishment and production costs together with yields through one production cycle of the pilot plantation. 3)Utilize the above data to develop a preliminary enterprise budget for Yaupon production based on actual establishment and production costs. 4) Begin development of an outreach plan for recruiting and training potential growers.Successful achievement of these objectives will result in the development of a baseline dataset of Yaupon properties that will be used to evaluate the effects of treatments and make a preliminary determination of financial feasibility. In addition, an outreach plan focused on small farmers will be developed with the assistance of extension and small farm specialists. Assuming a successful outcome to Phase I, Phase II will expand production research and optimize the production system through at least two additional cycles. Phase II will also evaluate the feasibility of an on-farm processing facility and fully develop outreach and training programs including a plan for mentoring new growers through their first production cycle. Phase III will commercialize the research by partnering with existing small farm outreach efforts to present the plan to growers with the goal of establishing 100 acres of Yaupon food production during the first three years.Benefits from a successful project include enhanced profitability and farm diversity for growers, a higher quality, more reliable source of Yaupon raw material for processors, enhanced consumer safety and satisfaction, and positive environmental effects through a reduction in the need to wild collect Yaupon to meet increasing market demand.
Animal Health Component
33%
Research Effort Categories
Basic
34%
Applied
33%
Developmental
33%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
10260301081100%
Goals / Objectives
In order to begin development of the needed data, the PI has established a pilot Yaupon plantation consisting of 10,000 plants across approximately 12 acres. The work plan of this Phase I SBIR proposal will utilize this planting plus expertise from Clemson University to determine the effect of shade and fertilization and evaluate the technical and financial feasibility of commercial production of Yaupon for its food value. Specifically, the project team will:1. Characterize clonal plantings of Yaupon on the YWC Pilot Farm from chemical and agronomic perspective.2. Compile plantation establishment and production costs together with yields through one production cycle of the pilot plantation.3. Utilize the above data to develop a preliminary enterprise budget for Yaupon production based on actual establishment and production costs.4. Begin development of an outreach plan for recruiting and training potential growers.}Successful achievement of these objectives will result in the development of a baseline dataset of Yaupon properties that will be used to evaluate the effects of future treatments and make a preliminary determination of financial feasibility. In addition, an outreach plan focused on small farmers will be developed with the assistance of extension and small farm specialists.Technical feasibility will be deemed to have been demonstrated when the baseline dataset has been established and data collected from one production cycle of the pilot plantation. Financial feasibility of the project will be deemed to have been demonstrated if the enterprise budget projections indicate a minimum farm gate price of $8 per dry pound will produce a gross income of at least $5,000 per acre by the fourth year after establishment.Assuming a successful outcome to Phase I, Phase II will replicate the research through at least two additional cycles providing multi-year data and conduct taste testing to determine the effect of the treatments on the final product. Phase II will also develop Good Agricultural Practices (GAP) compliant post-harvest handling and processing protocols, evaluate the feasibility of an on-farm processing facility, and fully develop outreach and training programs as well as a plan for mentoring new growers through their first production cycle. Phase III will commercialize the research by partnering with existing small farm outreach efforts to present the plan to potential growers with the goal of establishing 100 acres of Yaupon food production during the first three years of Phase III.}Benefits from a successful project include enhanced profitability and farm diversity for growers, a higher quality, more reliable source of Yaupon raw material for processors, enhanced consumer safety and satisfaction, and positive environmental effects through a reduction in the need to wild collect Yaupon to meet increasing market demand. Technical ObjectivesThe technical objectives and questions to be answered by this research are:1. Characterize clonal plantings of Yaupon on the YWC Pilot Farm from chemical and agronomic perspective.How are yield and chemical markers (Caffeine, Theobromine, and anti-oxidant potential {Hydrophillic Oxygen Radical Absorbance Capacity- ORAC}) affected by varying combinations of shade and fertilization?Does the plant form vary from shade and fertilization to facilitate or antagonize mechanical harvesting?Do desirable characteristics vary with harvest date?Successfully answering these questions will provide a preliminary basis for optimal cultivation of yaupon.2. Compile plantation establishment and production costs together with yields through one production cycle of the pilot plantation.What are the costs associated with establishing and producing Yaupon for food production?3. Utilize the above data to develop a preliminary enterprise budget for Yaupon production based on actual establishment and production costs.What is the total cost per acre to establish and maintain a Yaupon plantation for food purposes?How much income can a grower expect from one acre of Yaupon?Is the income competitive with other crops at the anticipated price level?Is it feasible for processors to pay the prices required for grower profitability?Successfully answering these questions will determine financial feasibility.4. Begin development of an outreach plan for recruiting and training potential growers.What is the best way to reach potential growers?Can we leverage existing small farm outreach programs in surrounding States?How can we effectively train new growers?}
Project Methods
Methods & Work Plan1. Characterize clonal plantings of Yaupon on the YWC Pilot Farm from chemical and agronomic perspective.Agronomic Characterization In Spring 2015, the PI established a Yaupon plantation on his farm located in Metter, GA consisting of 10,000 plants in rows 10 feet apart with 5 feet between plants for a total of approximately 12 acres.These plants were propagated by Innova Farms nursery in Boston, GA and delivered to the YWC farm in 5 gallon containers. The YWC Pilot Farm is currently transitioning to USDA National Organic Program certification.}?Upon notification of acceptance, Clemson University investigators will meet with members of Yaupon Wellness Company at the plantation in Savannah GA. An automated weather station with data capture on the farm will record temperature, humidity, rainfall and light. Temperature and light under the shade will be measured using handheld devices, comparing to the weather station in open field situation. Field plots will be laid out according to the experimental plan design below (see Figure 1 next page).Three nutrient applications rates with each level supplied by 4 rows of fertigation.Three shade levels (60%, 30% and 0), provided by two shade houses and an open space for 0, will run across the fertilizer treatments.Three harvest dates (May 31, July 15 and Aug 31) in the 2018 growing season.The completely randomized design, will be a full factorial, with repeated measures on each harvest date.Treatment 1. FertilizationTwelverows in each block will be fertigated using a Dosatron injection pump every two weeks withNeptune's Harvest Organic Hydrolyzed Fish Fertilizer (2-4-1). Emitters delivering 0.5 gallons per hour have been placed at each plant. The system will be activated for 1.5 hours per treatment resulting 0.75 gallons being delivered to each plant per treatment. Rows 1-4 will receive three ounces per gallon, rows 5-8 will receive one ounce per gallon and rows 9-12 will receive two ounces per gallon.Treatment 2. Shade30% shade will be constructed across the first 20 plants in all 12 rows. 60% shade will be constructed across plants 41-60. In rows 2,3; 6,7; and 10,11; plants 5-15; 25-35; and 45-55, will become the experimental plants. Therefore 20 interior plants, in all treatment factor blocks, will become the experimental units. The 20 sampled plants for each treatment block will be divided into 5 subplots of 4 plants each. Plant number 3 in each subplot is pre-selected, with 5 sentinel shoots on each shrub tagged for measuring shoot morphology. The tag will be placed 3 nodes before the terminal bud.On the harvest date, Clemson University and YWC Company staff will walk the experimental plots, making visual observation. Extension personnel will scout pests, diseases, or nutrient anomalies in the areas shown in Figure 1. The tagged sentinel shoots on the pre-selected shrubs will be severed 3 nodes above the tag removing new growth for that harvest period. Length of new growth, number of leaves, length of longest leaf on branch, leaf area (using Image J, https://imagej.nih.gov/ij/index.html) and thickness of longest leaf, will be measured and recorded for each sentinel shoot. The 5 shrubs in each subplot will be harvested by YWC Company farm crew with instruction of Clemson University personnel. Young shoots from each subplot will be placed in a paper sack Leaf fresh mass per subplot will measured and recorded. The sack will be slit open and contents laid flat, with 5 samples pulled from five precincts of the flattened shoot mass.The sampled shoots of each subplot will be separated into leaves and stems (a minimum of 20 g each sample) with the fresh weight recorded. Each sample will be assigned a unique identifier which will indicate the location from which the sample was taken. Samples from the even numbered subplots will be dried for 48 h at 40 C in paper envelopes. Dry mass will be determined and the harvested dry mass of each subplot will be estimated and recorded based on the % dry mass of the sample. These tasks will be performed by YWC employees and farm personnel at YWC Farm under supervision from Clemson personnel during the timeframe outlined above.All samples will be appropriately packaged and forwarded to Clemson University for chemical analysis and anti-oxidant potential testing. A complete set of tissue samples will also be kept at the YWC farm. Once the samples are received at Clemson, a hot water extract will be prepared from each dry samples for biochemical analysis. Two grams of dry leaf will be powdered using a ball mill. The powder will be steeped in 100 ml of boiled water held at a 100C for six minutes. The decoction will be centrifuged and the supernatant will be filtered through a 0.45µm nylon filter, and stored at -20C until analysis. RP-HPLC ProcedureCaffeine and theobromine concentration in the hot water extract will be quantified using Ultra High Pressure Liquid Chromatography (UHPLC) coupled to a Diode Array Detector. The compounds will be separated on a reverse phase column (Kinetic C18 XB, 3.0 x 150 mm, 2.6-μm particle size) using acetonitrile and 0.1% formic acid as the solvents. The compounds will be detected based on the absorbance at 274 nm, and the quantification will be done using the external standard curve prepared from commercial standards. Tentative identification of the separated components will be made by matching UV-Vis spectra and retention time match with commercial standards. Each sample will be analyzed in duplicates. The identity of the compounds in a set of subsamples will be further confirmed using an ultra-high resolution mass spectrometer (Orbitrap Fusion; Thermo Scientific) with an electrospray ionization interface following the above UHPLC condition. The identification will be based on accurate mass and fragmentation pattern of the compound/peak of interest.Hydrophilic-Oxygen Radical Absorbance Capacity (ORACFL) AssayA hydrophilic-ORACFL assay will be performed on the samples according to Robbins, Gong, Wells, Greenspan and Pegg (2015). A phosphate buffer (0.075 M, pH 7.4) will be used as the blank and diluent. Fluorescein (0.1 μM) will be employed as the reaction probe and 2,2′-azobis(2-amidino-propane) dihydrochloride, (AAPH, 80 μM in phosphate buffer), will be used as the radical initiator. Both working solutions will be held at 37 °C for the duration of the experiment. The phenolic extract will be diluted to 0.5 mg/mL with 95% (v/v) ethanol. The ethanolic solution will be further diluted with the phosphate buffer to a final concentration of 0.025 mg/mL. A standard curve based on five different Trolox concentrations (12.5, 25, 50, 80, and 100 μM in the phosphate buffer) will be constructed. The area under the kinetic curve (AUC) will be determined and following blank correction, samples and standards (i.e., 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid {Trolox}) will be compared. Final values will be reported as mmol Trolox eq./100-g sample from triplicate measurements.It is anticipated 108 assays will be required, 3 shade levels X 3 fertilization levels X 3 harvest dates for both stems and leaves (2), with 2 subplots in each treatment factor combination.

Progress 07/01/18 to 02/28/19

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? The Phase I activities of this project were designed to evaluate the effects of shade and fertilization on segments of a young yaupon plantation. The data collected would be used to develop a preliminary enterprise budget in order to determine the financial feasibility of cultivating yaupon holly for its food value. All objectives were successfully accomplished and the data shows that it is both technical and financial feasibility to cultivate yaupon for its food value. 1. Characterize clonal plantings of Yaupon on the YWC Pilot Farm from chemical and agronomic perspective. The field in Mettler GA was outfitted with shade cover and fertigation in April 2018. Three successive harvests were conducted June 13, July 25 and September 21, 2018 using harvest and sampling methods as described in the Work Plan. The early and late harvests had 2-3 times the mass of the July harvest for fresh and dry mass, across all treatment conditions. Total yields of the three harvests, by fresh and dry mass, are expressed as a pooled summation of harvest dates. The amount of harvested fresh mass (lb/acre) was affected by both the level of shading and the amount of fertilizer applied. Shading increased the fresh harvest mass at all fertility rates, and the increase was related to the rate of fertilizer applied (Fig 2A). At the standard fertility rate in full sun yielded about 925.9 lb/acre, and 30 and 60% shade both increased yield to about 1433 lb/acre. In 2x fertilizer application, 30% shading was ineffective and but 60% shading increased yield to about 1587.3 lb/acre. The regression curves indicate with standard fertilizer rate, shading at about 40% would be near optimal, but with 2x fertility it yield is likely to increase, with shading even greater than 60%. Treble fertilizer rate yielded less than 2x, and would have a greater cost. Yaupon teas are usually sold on a dry mass basis, and the yield in dry mass is an important measure of productivity. Harvested dry mass followed similar trends as fresh, with shading increasing the amount of harvested dry mass. At the standard fertilizer application dry mass increased from 385.8 to 551.2 lb/acre, moving from full sun to 30% shade (Fig. 2B), but shade greater than 30% is likely to decrease the yield of dry mass. With 2x fertilizer, 60% shade would be required to produce 551.2 lb/acre, and greater shading is likely to increase dry mass further. Treble fertilizer yielded no better than 2x, and would have a greater cost, for no real advantage. This plantation contained 1155 plants per acre, whereas a more standard density would be 2180 plants per acre (and a direct per plant adjustment would not be accurate). Yield of dry mass was improved using shade, and to a lesser extent fertilizer. 2. Compile plantation establishment and production costs together with yields through one production cycle of the pilot plantation. A preliminary enterprise budget for Yaupon production has been developed based on actual establishment and production costs based on 60% shading and 2x fertilization. Under these assumptions, the total cost per acre to establish and maintain a Yaupon plantation is estimated at $3820.18. This cost is comprised of $1837.23 maintenance costs (variable input costs), $203.61 of general overhead costs, and $1779.34 of establishment costs. Establishment costs are calculated by amortizing the establishment of the plants and infrastructure over a ten-year period. The cost of shading represents the largest fixed cost variable during establishment. This establishment cost estimate is based on the entire acreage covered with 60% shade. Unshaded or less shaded production would result in a lower establishment cost of $646.84 per acre (unshaded) and $304.90 lower for less shaded (30% shade) production. However, our data showed an approximately 40% improvement in yield by applying 60% shade (over unshaded) and an 11% improvement in yield by applying 60% shade versus 30% shade under the 2x fertilization scenario, which more than offsets the additional shade costs per acre over both the unshaded and 30% shade scenarios. To the extent that shading improves yield and quality of the product, the added cost of shading at or beyond 60% shade may prove justifiable in phase II of this grant. The range of gross income in this study ranged from a low of $3000 per acre at a low price/low yield scenario ($6 per pound/500 pounds) to a high of $8400 in a high price/high yield scenario ($12 per pound/700 pounds). The median/expected value is $4896 in this model at $8 per pound with a 612 pound yield. The gross income per acre is competitive with other specialty crops at the anticipated median price and yield level for dried, ground Yaupon tea. 3. Utilize the above data to develop a preliminary enterprise budget for Yaupon production based on actual establishment and production costs. Objective 4. Begin development of an outreach plan for recruiting and training potential growers. Most Land-Grant Universities operate New & Beginning Farmer and Sustainable Agriculture programs through their Cooperative Extension programs. The clientele served by these programs offer a natural target population and model for engagement of potential Yaupon growers. Additionally, once ideal growing regions are identified from both climatologic and supply chain perspectives, local County Extension offices, State Departments of Agriculture, and USDA offices can offer connections to potential growers and landowners. Because yaupon is a native plant that can be cultivated to organic standards, reaching out to landowners interested in low-impact crop production through local and regional conservation organizations, such as land trusts, could be another productive way to reach potential growers. Grower training will begin with the development of a Yaupon tea production guide. This guide will be illustrated with diagrams and photos to provide research-based recommendations on soil improvement practices, cultivar selection, plant spacing and planting guidelines, fertility recommendations, irrigation practices, pest management strategies (organic and conventional), shading and shade structure considerations, and harvesting guidelines. The published guide will be available to potential growers in electronic and print formats, and will be the basis for future Yaupon production trainings. Grower trainings will take two basic forms, 1) on-farm field days, and 2) grower workshops. Summary The results of Phase I produced baseline agronomic data that was used to develop an enterprise budget for commercial food production of Yaupon holly. That budget indicates that, at the median price/production level of $8 per pound/ 612 pounds per acre dry harvest, a gross income of $4,896 would be generated. Phase I technical feasibility was deemed to have been demonstrated when data had been compiled from a set of experiments designed to determine optimal production parameters. Financial feasibility would be indicated if the harvest data indicated income potential of $5,000 gross income from 1 acre of yaupon production. Therefore we submit that both technical and financial feasibility were fully achieved and that this information forms a good foundation for continuation and expansion of the research in Phase II and implementation by small growers in Phase III. The phytochemical data showed that shading clearly influenced the chemical profile by increasing caffeine content and lowering theobromine content. Although not a planned piece of the phase 1 grant, metabalomic analysis showed a wide variety of alkaloids, along with caffeine were up-regulated by shade. Similarly, many important phenyl-propanoid compounds were down regulated by shading. Therefore we must carefully analyze how environmental factors effect yield and quality. A taste panel will be convened to see how these documented changes make a difference in the human experience of the tea.

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