Progress 07/01/18 to 02/29/20
Outputs Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest? Using the results from the thermal studies and in vitro studies, an international patent filing was made as "Improved Ultra-Fast Cooling System and Methods of Use", PCT/US2019/26162, published as WO2019195791A1. The USPTO written opinion was that all claims are novel, involve inventive step, and industrially applicable. The PCT filing was being nationalized in USA, EU, Japan, China and India in 2021. The PI of this project is the leading inventor and the company owns the IP. CryoCrate was invited to presented the ultra-fast cooling technology and other CryoCrate technologies in Life Science Summit 2020, one of the twenty emerging company showcases, see: https://lifesciencessummit.org/presenters-20/. CryoCrate trademarked the technology as INSTAvitria®, and USPTO has granted us the trademark. CryoCrate published the associated technical details and applications on CryoCrate webpages What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts What was accomplished under these goals?
Our overall goal is to develop a novel ultra-fast cooling device to improve the efficiency of cryopreservation of animal embryos and spermatozoa. With the support of this SBIR award, we first designed and manufactured the proposed device system and tested the thermal performance and systematic operation, as proposed in Aim 1 and detailed in the Interim Technical Report: we designed and manufactured a novel two-phase, blade-jet-array device to achieve the desired ultra-fast cooling rate on a surface area that is significantly larger than that demonstrated in our preliminary results using a co-axial single jet device. We also designed and manufactured a cell suspension processing device that processes cell suspensions into ultrathin straws or ultrathin layers with large surface areas to be cooled by the liquid nitrogen jet arrays of the above ultra-fast cooling device. The device also forms closed systems that prevent contamination from liquid nitrogen. We tested the thermal performance of the proposed system composing two devices. We used the device system developed from the Aim 1 work to vitrify pig embryos and semen suspensions. We first determined the post-thaw viability of intact pig embryos and spermatozoa using standard in vitro assays. After confirming the success in vitrification of intact pig embryos (25% post-thaw re-expansion, which was five times more than the control group using traditional vitrification approach without delipidation), embryo transfers were performed on two sows, using 180 and 204 embryos vitrified by our device system, respectively. One of the two sows stopped menstruation and showed a wet oviduct. Approach and Results: In the Aim 1 work, 08/01/2018 to 02/28/2019, we successfully upgraded the proposed system through an iterative engineering approach combining theoretical analyses, numerical simulation and thermal performance measurement. A new design of cell suspension processing device was made and the devices were manufactured based on the new design. The thermal performance was tested and the systematic operation efficiency evaluated. In the Aim 2 work, 03/01/2019 to 02/29/2020, we performed standard in vitro or in vivo assays of post-thaw semen and embryos. The post-thaw re-expansion rate was over 25% for intact pig embryos, which was significant improvement of traditional approach of pig embryo vitrification without delipidation (less than 10% as re-expansion rate). However, in vivo ET experiments on two sows were not successful, due to technical reasons. We didn't observe any survival from thawed semen.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
https://lifesciencessummit.org/presenters-20/, see company CryoCrate
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Progress 07/01/18 to 06/30/19
Outputs Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?Patent publication, conference presentation, our website: Using the results from the thermal studies and in vitro studies, an international patent filing was made as "Improved Ultra-Fast Cooling System and Methods of Use", PCT/US2019/26162, published as WO2019195791A1. The USPTO written opinion was that all claims are novel, involve inventive step, and industrially applicable. The PCT filing was being nationalized in USA, EU, Japan, China and India in 2021. The PI of this project is the leading inventor and the company owns the IP. CryoCrate was invited to presented the ultra-fast cooling technology and other CryoCrate technologies in Life Science Summit 2020, one of the twenty emerging company showcases, see: https://lifesciencessummit.org/presenters-20/. CryoCrate trademarked the technology as INSTAvitria®, and USPTO has granted us the trademark. CryoCrate published the associated technical details and applications on CryoCrate webpages: What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts What was accomplished under these goals?
Our overall goal is to develop a novel ultra-fast cooling device to improve the efficiency of cryopreservation of animal embryos and spermatozoa. With the support of this SBIR award, we first designed and manufactured the proposed device system and tested the thermal performance and systematic operation, as proposed in Aim 1 and detailed in the Interim Technical Report: we designed and manufactured a novel two-phase, blade-jet-array device to achieve the desired ultra-fast cooling rate on a surface area that is significantly larger than that demonstrated in our preliminary results using a co-axial single jet device. We also designed and manufactured a cell suspension processing device that processes cell suspensions into ultrathin straws or ultrathin layers with large surface areas to be cooled by the liquid nitrogen jet arrays of the above ultra-fast cooling device. The device also forms closed systems that prevent contamination from liquid nitrogen. We tested the thermal performance of the proposed system composing two devices. We used the device system developed from the Aim 1 work to vitrify pig embryos and semen suspensions. We first determined the post-thaw viability of intact pig embryos and spermatozoa using standard in vitro assays. After confirming the success in vitrification of intact pig embryos (25% post-thaw re-expansion, which was five times more than the control group using traditional vitrification approach without delipidation), embryo transfers were performed on two sows, using 180 and 204 embryos vitrified by our device system, respectively. One of the two sows stopped menstruation and showed a wet oviduct.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
https://lifesciencessummit.org/presenters-20/
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