Progress 07/01/09 to 06/30/14
Outputs Target Audience: Target audience for this work is first, meat processors, then other meat and food scientists, livestock and poultry producers and consumers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? The completion of these studies provided opportunities for training a Master's Degree student and two undergraduate research projects. How have the results been disseminated to communities of interest? Results were presented in Abstract form at professional meetings. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts What was accomplished under these goals?
Studies were conducted to evaluate the impact of low oxygen atmospheres for increasing meat oxidative stability in a Master Pack system. With oxygen concentrations of 1 to 3% fresh beef color was impaired. To assure very low oxygen concentrations and avoid formation of metmyoglobin, oxygen scavengers were utilized in master packs. For this, a series of experiments were performed to show the effect of storage time and presence of oxygen scavengers in 20% carbon dioxide and 80% nitrogen (20/80) and 30% carbon dioxide and 70% nitrogen (30/70) master packed ground beef. Oxygen scavengers proved to be beneficial for maintaining a very low oxygen concentration (less than 0.1%) and desirable fresh beef color. Nevertheless, their effect on depletion of carbon dioxide and formation of carbon monoxide must be considered as well. An abstract based on this work was presented at the Reciprocal Meat Conference held in Madison, Wisconsin.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Harvison, K.J. and E.W. Mills. 2014. Modified Atmosphere Packaging Affects Ground Beef Patty Cohesiveness. Proceedings of Reciprocal Meat Conference 67:18.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Arteaga, I.S. and E.W. Mills. 2014. Oxygen Scavengers Affect Color and Gas Concentrations of Master Packed Ground Beef. Proceedings of Reciprocal Meat Conference 67:1064.
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Progress 10/01/12 to 09/30/13
Outputs Target Audience: Target audience is meat processors, other meat scientists, livestock producers and consumers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? Work in this project area provided for successful completion of a doctoral degree in Animal Science, ongoing training of a Masters Degree student and several undergraduate student research opportunities. How have the results been disseminated to communities of interest? Results have been presented in Abstract form at professional meetings. What do you plan to do during the next reporting period to accomplish the goals? Work will continue toward publication of natural cured bacon results. Meat packaging investigations will be continued and developed into abstract or peer reviewed publications.
Impacts What was accomplished under these goals?
Work in this project area lead to submission and acceptance of PhD thesis investigating the application of natural curing processes in the production of bacon. Abstracts were presented at professional meetings and manuscripts are in preparation for submission. Preliminary trials were completed to investigate the impact of packaging environment on texture of ground beef patties.
Publications
- Type:
Other
Status:
Awaiting Publication
Year Published:
2013
Citation:
Mills, E.W. 2013. Additives and Extenders. in Encyclopedia of Meat Science. Elsevier Science, Kidlington, Oxford, UK. Vol. 1:1-6.
- Type:
Other
Status:
Awaiting Publication
Year Published:
2013
Citation:
Mills, E.W. 2013. Functional Ingredients. in Encyclopedia of Meat Science. Elsevier Science, Kidlington, Oxford, UK. Vol. 1:6-11.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2013
Citation:
McKeith, A.G., E.W. Mills, C.N. Cutter, K.B. Kephart. 2013. Inhibition of Foodborne Pathogens inNo-Nitrate or Nitrite-Added Bacon. Proceedings of Reciprocal Meat Conference 66:49-50, (abstract number 106).
- Type:
Journal Articles
Status:
Accepted
Year Published:
2013
Citation:
Svoboda, A., C. DebRoy, E. Dudley, E. Mills, C. Cutter. 2013. Presence of Shiga Toxin-Producing Escherichia coli O-Groups in Small and Very Small Beef Processing Plants and Resulting Ground Beef Detected by a Multiplex Polymerase Chain Reaction Assay. Foodborne Pathogens and Disease, Vol. 10, accepted for publication
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Magwedere, K., H.A. Dang, E.W. Mills, C.N. Cutter, E.L. Roberts and C. DebRoy. 2013. Incidence of Shiga toxin-producing Escherichia coli strains in beef, pork, chicken, deer, boar, bison, and rabbit retail meat. J VET Diagn Invest 2013 25:254-258.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2012
Citation:
Gipe, A. 2012. Investigation of Quality Attributes And Inhibition of Foodborne Pathogens in No-Nitrate or Nitrite-Added Bacon. PhD Thesis, Penn State University, University Park PA.
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Progress 10/01/11 to 09/30/12
Outputs OUTPUTS: Ingoing nitrite concentration is a primary determinant of ultimate cured meat properties and cured meat safety. Natural ingredients such as celery juice powder provide nitrite via microbial reduction of nitrate prior to or during manufacture of "no-nitrate or nitrite-added" meat products. However, such ingredients have variable nitrate contents. Therefore, the objective of this study was to investigate the nitrate and nitrite contents of "no-nitrate or nitrite-added" bacon brine formulations. Brines were all manufactured with 5% sugar. All no salt (NS) treatments were formulated with no added salt and all salt (S) treatments were formulated with 20% salt. The curing ingredients investigated are vegetable juice powder with starter (V) (natural nitrate; treatments NSV2 (2%), NSV4 (4%), SV2 (2%), SV4 (4%)), pre-converted celery juice powder (C) (natural nitrite; treatments NSC, SC), and pre-converted celery juice powder with natural cure accelerator (CC) (natural nitrite with accelerator; treatments NSCC, SCC). All commercial ingredients were utilized at concentrations recommended by the supplier. Conventional-cure brine (NI) (treatments NSNI, SNI) contained 1200 ppm sodium nitrite and 5500 ppm sodium erythorbate. The No-Cure brine (NC) (treatments NSNC, SNC) contained sugar (5%) and water and salt (0 or 20%) respectively. Results demonstrated that treatments NSV2, NSV4, SV2, and SV4 had lower (P < 0.05) pHs than all other treatments (NSC, NSCC, NSNI, NSNC, SC, SCC, SNI, SNC). As expected, conventional treatments (NSNI and SNI) had greater (P < 0.05) nitrite concentrations than all other treatments. In addition, treatments NSV2 and NSV4 had increasing nitrite concentrations over the first 6 hours then decreased thereafter. Additionally, nitrate concentrations were higher in treatments NSV2 and NSV4 (no salt, natural nitrate with starter culture) than all other treatments. These data suggest that salt may inhibit the reduction of nitrate when pre-incubating brines containing natural nitrate. Pre-incubating the brine for 4-6 hours, without salt, prior to injection, may help manufacturers eliminate the need for an incubation step during the cooking/smoking process and shorten the time needed for processing "no-nitrate or nitrite-added bacon. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: The target audience for this information is academic, industry and government meat scientists. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts The findings of this work demonstrated that nitrate and nitrite contents are quite variable in curing brines and cured products prepared using natural curing ingredients. The study results were presented at one national and one international research conference where academic, industry and government scientists received the information. In this country the USDA, Food Safety Inspection Service is actively collecting information in order to determine how best to regulated labeling of naturally cured meat products.
Publications
- Svoboda, A., C. DebRoy, E. Dudley, E. Mills, and C. Cutter. 2012. Presence of Shiga-toxin Producing Escherichia coli in Small and Very Small Beef Processing Plants and Resulting Beef Products Detected by a Multiplex Polymerase Chain Reaction Assay. Providence, RI. IAFP (Abstract #P3:16).
- Gipe, A. N., E. W. Mills, C. R. Raines, K. B. Kephart, and C. N. Cutter. 2012. Investigation of quality attributes of no-nitrate or nitrite-added bacon. Proceedings of Reciprocal Meat Conference 65:58-59. (Abstract #28).
- Yoder, S. F., W. R. Henning, E. W. Mills, S. Doores, N. Ostiguy, and C. N. Cutter. 2012. Investigation of chemical rinses suitable for very small meat plants to reduce pathogens on beef surfaces. J. Food Prot. 75:1 pp. 14-21.
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Progress 10/01/10 to 09/30/11
Outputs OUTPUTS: The consumption of a wide variety of species of wild and farmed game has been an important source of protein for humans world-wide for millennia. Shiga toxin producing Escherichia coli (STEC) has emerged as important enteric food-borne zoonotic pathogen of considerable public health significance worldwide but little is known about the level of contamination in game meat. The main game meat consuming populations are the traditional ones with most of the game meat produced and processed for their own consumption under regulatory exemption thereby escaping sanitary scrutiny in many countries. In this study, a total of 55 samples from five game species (deer, wild boar, bison, rabbit and elk) were collected from retail game meat vendors in Pennsylvania for detection of eight STEC serogroups (O26, O45, O103, O113, O111, O121,O145 and O157) that are responsible for the majority of STEC infections in domestic livestock and livestock products using a multiplex PCR assay method. The method for detection of STEC consisted of enrichment in modified tryptic soy broth (mTSB) and static incubation for 6 h at 37 C initially and 18 h at 42 C subsequently, followed by Deoxyribonucleic acid (DNA) extraction with the MasterPure DNA Purification Kit. Multiplex polymerase chain reaction (PCR) assays targeting the wzxgene in the O-antigen gene clusters of the eight serogroups and the stx1, stx2, genes for the wzx positive samples was done. Seven serogroups were detected with the exception of O26. One white tailed deer ground meat sample was confirmed positive for Stx1 gene. The result shows the importance of microbiological testing and performance standards as a critical part of commercial game meat oversight and the need for increased surveillance of wild game meat. PARTICIPANTS: Much of the work on this project was completed by Dr. Kuda Magwadere a visiting scientist from Namibia who was sponsored by the Borlaug program of the USDA, Foreign Agricultural Service. TARGET AUDIENCES: The findings of this work are of significance to meat processors and meat consumers especially those involved with commercial game meat. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts Farm raised game animals are typically harvested in facilities similar to those used for USDA inspected slaughter of amenable livestock species. However, regulatory oversight of such facilities is much less stringent than for USDA inspected plants. This project was conducted as a preliminary survey of game meat sold through a number of market channels. The discovery numerous viable STEC serogroups in game meat samples suggests frequent fecal contamination. Thus there is an opportunity to improve the safety of game meat products by improving sanitary practices applied at and after slaughter. Since the current project only dealt with retail products obtained at specialty stores and farmer's markets no firm conclusions may be drawn as to the specific source of the contamination. Additional investigations are required at the slaughter facilities in order to identify the sources and better focus future efforts on prevention.
Publications
- Jin, J. and E. W. Mills. 2011. Split phalanges from archaeological sites: Evidence of nutritional stress Journal of Archaeological Science 38(8):1798-1809.
- Yoder, S. F., W. R. Henning, E. W. Mills, S. Doores, N. Ostiguy, and C. N. Cutter. 2010. Investigation of water washes suitable for very small meat plants to reduce pathogens on beef surfaces. J. Food Prot. 73:907-915.
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Progress 10/01/09 to 09/30/10
Outputs OUTPUTS: The benefits of improved diet are especially important in communities and households where food resources or capital for food purchases are limited. The Lisha Bora research and educational program (NIH funded work underway in Eldoret, Kenya) emphasizes the importance of good nutrition for health and wellbeing. Lisha Bora is an example of the continuing effort to improve nutrition in part by increasing the use of meat in nutrient deficient diets. The purpose of the current work is to assess agricultural, political, infrastructural, financial, and community prospects for sustainable production of meat and food containing meat in Kenya. Agricultural: The southern regions of Kenya are agriculturally productive areas with considerable current production and good potential for further integration of livestock and crop production. Maize and wheat are commonly raised on large holder farms. Small holder farms typically produce maize along with a wide variety of other crops. Concentrated poultry production was observed at several sites with flocks of about 200 birds being raised using modern technology and formulated feed leading to rapid growth of meat birds or increased egg production. Political: Federal and local governments are currently reasonably stable, providing some assurance that investors will be able to realize the benefits of their creative and financial commitments. However, tribal influences are still strong across much of Kenya. Infrastructure: All weather roads are present in some areas but most rural areas are accessible only by dirt roads and may be inaccessible during rainy seasons. Paved roads between major cities, while being improved, are not well maintained and may be nearly impassible in places. Electricity is available in cities and larger towns but is not common on homesteads in rural areas. The public water supply, if available, is typically unreliable and water is commonly managed by individual households or business establishments. Financial: Faulu Bank in Eldoret, Kenya is an example of a financial services provider that also provides education and technical training for its customers. Faulu arranges group/community training sessions on topics relating to group finance, enterprise development and technology applications. Focused technology topics include mushroom production, yogurt production, baking, liquid soap production and fruit juice extraction. Community: The system of household financial decision making will be an important influence on the ease/comfort with which someone might try a new activity. For women, the ability to engage in group income generating activities protects their resources from being confiscated by spouses or other family members. Many types of groups are already established in Kenyan communities. Existing community groups are often established by individuals with common enterprise or commodity interests. Groups may be composed of vegetable producers, dairy farmers, goat herders or rabbit producers to name a few. These groups represent a natural starting point as we work to identify prospects for NutriBusiness development. PARTICIPANTS: Participants in this project included research leader Dr. Edward Mills, PSU, Department of Dairy and Animal Science. TARGET AUDIENCES: Benefits of this work will accrue to undernourished and frail individual in developing countries. International aid agencies and governments might adopt this technology or model other products on the concepts developed here. Some product concepts might be utilized in snack food production in the developed world. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts Work in Kenya in assessing prospects for increased local production of meat animals and community level processing and manufacturing was conducted and a project report was submitted to sponsors at Indiana University and the Global Livestock CRISP. Presentations were made to both governmental and non-governmental organizations in Kenya. Cooperative efforts with Heifer International are currently underway to develop a goat based Nutribusiness enterprise in Homabay Kenya. Cooperation is being initiated with Jomo Kenyatte University in Nairobi for further development of Chiparoo products.
Publications
- Steinberg, E. L., J. W. Comerford, V. H. Baumer, and E. W. Mills. 2009. Production and consumer characteristics of pasture-fed beef. Prof. Anim. Sci. 25:443-448.
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Progress 07/01/09 to 09/30/09
Outputs OUTPUTS: The benefits of improved diet are especially important in communities and households where food resources or capital for food purchases are limited. Young children and those who are frail due to illness or age are especially vulnerable to reduced access to adequate nutrition. The Nutribusiness model for a value-added food processing enterprise was developed with the idea that women in a group that develops a food production, processing and marketing business would also be able and inclined to provide improved nutrition for their children and others within their care. By design, Nutribusiness enterprises formulate and manufacture nutritionally superior foods and generate income for Nutribusiness owners. Chiparoo is a product developed for production in a Nutribusiness enterprise. It contains a broad range of important nutrients due to a combination meat and nonmeat ingredients. In its original form it is intended to serve as a snack for children or an adjunct to be crumbled into a weaning porridge for very young children. As such it could provide meat source nutrients in the diets of young children or others who would not otherwise be provided with meat. Lisha Bora research and educational program (NIH funded work underway in Eldoret, Kenya) emphasizes importance of good nutrition for health and wellbeing. Continued progress for improving nutrition can be achieved by improving knowledge of nutrition and proper food selection, increasing agricultural production and successful enterprise development leading to improved economic status. Assessment of possible alternatives for local meat production and processing in Kenya is in progress. PARTICIPANTS: Participants in this project included research leader Dr. Edward Mills, PSU, Department of Dairy and Animal Science. TARGET AUDIENCES: Benefits of this work will accrue to undernourished children and frail adults in developing countries. International aid agencies and governments might adopt this technology or model other products on the concepts developed here. Some product concepts might be utilized in snack food production in the developed world. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts Work in Kenya in assessing prospects for increased local production of meat animals and community level processing and manufacturing is underway. Further research is being planned to address food safety and flavor problems encountered. Considering the short time since the start of the project, July 1, 2009, no specific outcomes are reported at this time.
Publications
- Mills, E.W. 2009. Meat Product Safety. J. Anim. Sci. Vol. 87. E-Suppl. 2/J. Dairy Sci. Vol. 92. E-Suppl. 1:537.
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