Source: UNIVERSITY OF GUAM UOG STATION submitted to
DETERMINATION OF SHELF-LIFE, QUALITY, AND SAFETY OF LOCALLY-GROWN FRESH-CUT PRODUCES ON GUAM
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
1009206
Grant No.
(N/A)
Project No.
GUA0908
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Mar 29, 2016
Project End Date
Sep 30, 2019
Grant Year
(N/A)
Project Director
Yang, JI, .
Recipient Organization
UNIVERSITY OF GUAM UOG STATION
(N/A)
MANGILAO,GU 96913
Performing Department
Cooperative Extension Service
Non Technical Summary
Guam greatly depends on imports in agricultural products. About 90% of foods are imported from Asian countries and U.S. mainland. Due to long-distance international transportation, the produces consumed by residents are often not farm-fresh, easily spoiled, and poor in quality. Replacing 10% of imports of foods with locally grown fresh produces and processed products is a goal to change the situation. Guam can cultivate more than one hundred kinds of vegetables and fruits. Wisely processing these tropical and subtropical crops is one of strategies to address the critical issue.Applying modified atmosphere packaging, vegetables and fruits can be processed into safe, farm-fresh, healthy, and convenient products¾fresh-cut produces. The objectives of the project are to: (1) determine the optimum post-harvest storage and processing conditions on the safety and quality of fresh-cut produces; (2) determine the shelf-life of fresh-cut produces based on sensory, nutritional, and microbial quality; and (3) delivery science-based knowledge of processing fresh-cut produces in the community. We expect that the project will promote processing and marketing fresh-cut produces on the island; benefit farmers, food processors, residents, and consumers; and reduce the food imports and improve the agricultural sustainability on Guam.
Animal Health Component
0%
Research Effort Categories
Basic
30%
Applied
50%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
5025010101030%
5015010100030%
7125010110030%
5125010101010%
Goals / Objectives
The major goal of this project is to use available tropical and sub-tropical food resources on Guam to process and market fresh-cut produces to benefit residents and consumers with "safe, healthy, fresh, and convenient" products, reduce high food imports, and improve the agricultural sustainability on the island.The objectives of this project are to:Determine the optimum cultivar, maturity or ripeness, and post-harvest storage of locally-grown vegetables and fruits for processing high quality fresh-cut produces;Determine optimum conditions for processing selected fresh-cut vegetables and fruits;Determine the respiration rate of fresh-cut produces for the optimum conditions of modified atmosphere packaging;Determine the shelf-life of fresh-cut produces by evaluating sensory, nutritional, and microbial quality;Develop extension educational curriculum and conduct workshops in the community to teach target audiences to process and market fresh-cut produces.This project will address the USDA National Priority and Outcomes:Local and Global Food Security and Hunger, including Outcome #1 "Enhanced capacity of a sustainable global food system", Outcome #2 "More sustainable, diverse, and resilient food systems across scales", and Outcome #3 "Improved local, national and global capacity to meet growing food demands";Food Safety, including Outcome #1 "Increase number of viable technologies to improve food safety", Outcome #2 "Reduce incidence of foodborne illness", and Outcome #3 "Increase adoption of recommended safe food handling practices at the individual, family, community, production, and supply system levels".
Project Methods
Materials The selected fresh vegetables and tropical fruits will be harvested and obtained from local farmers or purchased from local markets. After harvest, the vegetables and fruits will be directly sent to Food Science Laboratory at College of Natural and Applied Sciences and stored properly before processing.Fresh-cut processing The procedures for preparing fresh-cut produces depend on the variety of vegetables and fruits. Generally, the vegetable or fruit will be first cleaned, sanitized, and washed and then trimmed or peeled, decored, and cut. If needed, the fresh-cut vegetables and fruits may be treated with certain solutions (such as ascorbic acid, calcium lactate, citric acid) to prevent browning and softening during storage. The prepared fresh-cut produces will be immediately used for modified atmosphere packaging.Modified atmosphere packaging (MAP) Based on the respiration rates of fresh-cut produces and the permissive rates of packaging film for O2, CO2, and H2O (moisture), specific trays and bags (or containers) and MAP film will be selected to maintain the required conditions for modified atmosphere packaging. The selected final O2 and CO2 concentrations in MAP will be achieved to maximally extend the shelf-life of fresh-cut produces. The modified atmosphere packaged tropical fruits will be stored at the temperature of 1-4 °C.Quality evaluation The quality of fresh-cut produces during storage for 10-14 days will be evaluated using analytical methods and sensory evaluation by observing their appearance and determining their sugar content, acidity, color, firmness, flavor, and taste. The nutrition quality of fresh-cut produces (such as ascorbic acid, total phenolics, total flavonoids, and key phytochemicals) will also be determined analytically.Microbial quality and safety The microbial quality will be determined by measuring standard plate counts, molds and yeasts, and coliforms of fresh-cut produces. To ensure food safety of fresh-cut fruits, the microbial challenging test will be used to determine the survival and reduction of inoculated E. coli and Listeria innocua.Shelf-life determination The shelf-life of tropical fresh-cut produces with modified atmosphere packaging during storage will be determined based on the product quality and microbial quality of fresh-cut produces. The limit for the quality loss of fresh-cut produces to determine the shelf-life will not exceed 30% of the original quality of fresh-cut produces during storage.Statistical analysis Three replications of each treatment were performed for all experiments. One-way analysis of variance and least-significant-difference test will be conducted to identify differences among means. Mean differences will be considered significant at the P<0.05 level.

Progress 03/29/16 to 09/30/19

Outputs
Target Audience:Farmers, enterpreneurs, educators,consumers, and students. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We presented the results of the study on the fresh-cut coconut flakes and fresh-cut breadfruit in the 9th and 10th University of Guam Conference on Island Sustainability in 2018 and 2019, respectively. The technology of processing fresh-cut breadfruit using modified atmosphere packing was delivered in the community workshop "Fresh-cut Breadfruit". In addition, the technology was also taught in the class of Food Processing Technology at University of Guam. How have the results been disseminated to communities of interest?Information about the shelf-life of fresh-cut breadfruit was provided tofarmers and entrepreneurswho are interested in breadfruit plantation, processing, and marketing inthe Pacific islands. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Coconut and breadfruit are popular tropical fruits on Guam. However, the coconut meat and fresh breadfruit have a short shelf-life of 3-5 days even during storage in refrigerator. In this project, we used the modified atmosphere packaging (MAP) to study the shelf-life of coconut meat and fresh-cut breadfruit. The vacuum packaging was also used for coconut meat in the study. During storage of coconut meat at 4 °C for 16 days, we tested the weight loss, pH value, total soluble solids, color value, aerobic plate counts, and molds and yeasts of coconut meat and also determined the O2 and CO2 concentration in the packaging. During storage of fresh-cut breadfruit at 4 °C for 3 weeks, we determined the color values, texture, and microbial counts of fresh-cut breadfruit and also analyzed the concentrations of O2, CO2, and C2H4 in the polyethylene bags with MAP. We found the optimum processing for coconut meat was using vacuum packaging to pack fresh-mature coconut meat without blanching treatment. This processing can extend the shelf-life of coconut meat for 16 days at 4 °C. In the studying of fresh-cut breadfruit, we observed that the modified atmosphere packaging can extend the shelf-life of breadfruit to 14 days at 4 °C. The fresh-cut breadfruit during storage within 14 days exhibited a good quality for steaming or other uses in food preparation. The shelf-life of processed coconut meat under vacuum and the fresh-cut breadfruit with MAP at 4 °C can meet the needs for distributing and marketing the coconut and breadfruit products on Guam and otherPacific islands.

Publications

  • Type: Other Status: Published Year Published: 2019 Citation: Yang, J. and De Jesus, J. 2019. Extending the shelf-life of fresh-cut breadfruit (Artocarpus altilis) by modified atmosphere packaging. The 10th University of Guam Conference on Island Sustainability. Session 2C. Presentation Abstract.


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Entrepreneurs, farmers, general consumers,educators, and students. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We presented the results of the study on the shelf-life of fresh-cut breadfruit in the10th University of Guam Conference on Island Sustainability in 2019. The technology of processing fresh-cut breadfruit using modified atmosphere packing was delivered in the community workshop "Fresh-cut Breadfruit". In addition, the technology was also taught in the class of Food Processing Technology at University of Guam. How have the results been disseminated to communities of interest?Information about the shelf-life of fresh-cut breadfruit was provided tofarmers and entrepreneurswho are interested in breadfruit plantation, processing, and marketing inthe Pacific islands What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? We processed the fresh-cut mature breadfruit and used the modified atmosphere packaging to pack the fresh-cut breadfruit in polyethylene bags. The color, texture, gases (O2, CO2, and C2H4), microbial counts, and molds & yeasts of fresh-cut breadfruit were analyzed during the storage of fresh-cut breadfruit at 4 °C for three weeks. In addition, we tested the sensory quality of steamed fruit-cut breadfruit. We observed that the color a* and b* values were constant and the L* value decreased about 30% within the first 10 days. The firmness of fresh-cut breadfruit did not change during storage for three weeks. The oxygen level in the modified atmosphere packaging decreased to 3% at storage of 10 days, the carbon oxide level increased to 20% after storage of two weeks, and the ethylene level significantly enhanced after two weeks. The total plate counts of fresh-cut breadfruit were below 106 during storage of two weeks. The sensory quality of steamed fresh-cut breadfruit was accepted within two weeks of storage. Based on the results, the fresh-cut breadfruit with modified atmosphere packaging showed a shelf-life of 14 days at 4 °C. The shelf-lifeof fresh-cut breadfruit can meet theneeds ofprocessing, distributing, and marketing the productin the Pacific islands.

Publications

  • Type: Other Status: Published Year Published: 2019 Citation: Yang, J. and De Jesus, J. 2019. Extending the shelf-life of fresh-cut breadfruit (Artocarpus altilis) by modified atmosphere packaging. The University of Guam 10th Conference on Island Sustainability. Session 2C. Presentation Abstract.


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Farmers, entrepreneurs, food processors, residents, and educators Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The project trainedtwo reseach associates in the area of processing fresh-cut food products using modified atomosphere packaging, determining the shelf-life and quality of theproducts. How have the results been disseminated to communities of interest?Some results from the project were presented in a local conference "2018 Island Sustainability Conference" at University of Guam. What do you plan to do during the next reporting period to accomplish the goals?We will continute conduct research experiments to study the shelf-life of fresh-cut breadfruit using modified atmosphere packaging and delivere the science-based information in the community through extension outreach programs.

Impacts
What was accomplished under these goals? We conducted experiments to accomplish the objective1, 2, 3, and 4. We selectedcoconut fruit and breadfruitfruitas locally grown tropical fruitsfor fresh-cut products. Weinvestigated the modified atmosphere packaging for fresh-cut breadfruit and coconut-meat products and study the shelf-life and quality offresh-cut breadfruit duirng storage at 4º C.We determined the respiration(ethylene, O2, CO2) and microbial counts of fresh-cut breadfruit in modified atomosphere packaging during storage at 4ºC for 3 weeks.

Publications


    Progress 10/01/16 to 09/30/17

    Outputs
    Target Audience:Food scientists, food technologists, food entrepreneurs, farmers, and consumers. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Tropical breadfruit is a unique, nutritious, and healthy fruit on Guam, we plan to test the shelf-life of minimum-processed breadfruit in the next reporting period.

    Impacts
    What was accomplished under these goals? Coconut is popular tropical fruit on Guam; residents use fresh mature coconut meat for extracting coconut milk and cooking. To extend the self-life of fresh coconut meat, we minimum-processed the coconut meat by grating, blanching, packaging using passive modified atmosphere packaging (MAP) or vacuum in polyethylene bag, and storing at 4 °C for 16 days. During the storage, the packaged coconut meat was sampled at 0, 4, 8, 12, 14, and 16 days for determining the weight loss, pH value, total soluble solids, color value, aerobic plate counts, and molds and yeasts as well as O2 and CO2 concentration in packaging. Two replications were performed for each experiment. The unblanched and blanched coconut meat exhibited a pH value of 6.4-7.0, which did not significantly change during storage for 16 days at 4°C with the MAP and vacuum packaging. The blanched coconut meat exhibited total soluble solids of 3.0 °Brix less than that of unbalanced coconut meat at 7-9 °Brix, showing a significant loss in soluble solid content of the grated-coconut meat during blanching. The MAP and vacuum packaging did not significantly change the total soluble solids of coconut meat for 16 days at 4°C. Although the weight of packaged coconut meat did not change during the first 8 days of storage, the weight loss increased gradually at 8-12 days. At day 16, the vacuum and MAP packaged coconut meat exhibited a weight loss of 4.5% and 1.5%, respectively. The coconut meat exhibited a slight increase in darkness and color intensity during storage of 16 days at 4 °C by decreasing the color value L* (10-15%) and increasing the hue value (5-11%). During storage at 4 °C for 16 days, the aerobic plate counts and the counts of molds and yeasts of the coconut meats increased 2-3 log and 5 log, respectively, except for the blanched coconut meat with the MAP packaging, which exhibited a 10-log increase in molds and yeasts. Except for the MAP-packaged blanched coconut meat, the total plate counts and molds and yeasts did not exceeded an increase of 6-log during the storage for 16 days for the vacuum-packaged blanched and nonblanched coconut meat and for the MAP-packaged nonblanched coconut meat. For the MAP-packaged blanched coconut meat, the atmosphere of the packaging exhibited an increase of 2.7-6.6% in CO2 concentration and a decrease of 5-12% in O2 concentration at the day 14-16, suggesting a significant growth of microorganisms. The results of the study suggested that the vacuum packaging without blanching is a good processing to extend the shelf-life of fresh coconut meat for storage at 4 °C and the shelf-life can be extended to 16 days. The MAP packaging with blanching is not suggested to extend the shelf life of fresh coconut meat because blanching decreased the total soluble solids of coconut meat and bacteria, molds, and yeasts in blanched coconut meat grew aggressively in the MAP packaging compared with the vacuum packaging.

    Publications


      Progress 03/29/16 to 09/30/16

      Outputs
      Target Audience:The target audiences include farmers, food facilities, entrepreneurs, and individuals. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?We plan to study other tropical vegetables and fruits for processing fresh-cut produce. We willdetermine the respiration rate of fresh-cut produces for the optimum conditions of modified atmosphere packaging andthe shelf-life of fresh-cut produces by evaluating sensory, nutritional, and microbial quality;

      Impacts
      What was accomplished under these goals? At the initiate stage of the project, we purchased instrument such asa gas analyzer, poly bag sealer,bags for modified atmoshper packaging, and suppliesfor preparing the study. We selected cucumber and romaine lettucefor preliminarystudy usingmodified atmospher packaging.Thecucumbers and romain lettuce were cleaned, washed with antimicroibal agent, cut, and packed withmodfied atmospher packaging.The qualityand shelf-life of the fresh-cut produce were evaluated during storage at 4ºC for two weeks. The packaged fresh-cut cucumber and romain lettuce showed good freshness andquality in color and textureduringstorage for7-10 days at 4ºC.

      Publications