Source: UNIVERSITY OF WYOMING submitted to NRP
NEW, EMERGING, AND RE-EMERGING ANIMAL DISEASES: WYOMING AND THE INTERMOUNTAIN REGION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1008910
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Dec 8, 2015
Project End Date
Sep 30, 2020
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF WYOMING
1000 E UNIVERSITY AVE DEPARTMENT 3434
LARAMIE,WY 82071-2000
Performing Department
Veterinary Sciences
Non Technical Summary
Hatch and Animal Health projects in the Department of Veterinary Sciences (DVS) and the Wyoming State Veterinary Laboratory (WSVL) are interwoven. In the past the projects have been combined. Currently, separating these projects is justified but there remains overlap in the scope of work. The expertise in animal diseases in DVS and the WSVL has focused on animal health issues that occur at the wildlife/livestock interface. This focus remains an imperative as some diseases impact the economic viability of livestock production and producers. With the acceptance of the One-Health initiative during the last few years and the realization that the health of animals, humans, and the environment are interrelated, this focus will expand to diseases that occur at the wildlife/livestock/companion animal/human interface. Large land holdings are being subdivided throughout Wyoming and the populace, often individuals from larger urban centers, is expanding into rural and semi-rural areas. The expansion will lead to greater exposure of the populace to zoonotic diseases and environmental toxins. The WSVL, operated and staffed by personnel of DVS, is an AAVLD-accredited full-service laboratory involved in the timely and accurate diagnosis of animal disease and is the only diagnostic laboratory of its kind in Wyoming. Additionally, the WSVL is a member of the National Animal Health Laboratory (NAHLN, USDA) and Laboratory Response Networks (LRN, CDC) and is approved to test for high-impact animal pathogens as well as those that can affect human health. These are pathogens that can occur naturally or that could be introduced as an incident of bioterrorism. The focus of DVS and the WSVL is not only on infectious disease. Serious animal losses occur from hereditary causes and from toxins; furthermore, animals can serve as sentinels of environmental degradation. Early recognition, diagnosis, and characterization of new, emerging, or re-emerging diseases in animals can help mitigate the economic impact of diseases to livestock enterprises, monitor the health of wildlife populations, and insure the health of humans and the environment. However, early recognition, diagnosis, and characterization require the acquisition and retention of well trained staff, sophisticated equipment, state-of-the-art procedures, and other resources. This infrastructure must be in place to react in a timely fashion to the unusual occurrence of a novel animal disease. During real-world outbreaks, there is seldom time to accumulate the necessary resources to mount an effective, rapid response. To this end, the WSVL has close working relationships with the Wyoming Livestock Board (WLSB, which includes the office of the state veterinarian), USDA, Wyoming Department of Health (WDH), and the Wyoming Game and Fish Department (WGFD). Knowledge gained and lessons learned during real-world disease outbreaks, in their natural setting, are among the most cost effective forms of applied research for the public sector and in the public interest (D O'Toole: Monitoring and investigating natural disease by veterinary pathologists in diagnostic laboratories. Veterinary Pathology 47: 40-44, 2010).
Animal Health Component
80%
Research Effort Categories
Basic
20%
Applied
80%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3113910110020%
3113910110120%
3113910111010%
3113910116030%
3113910115010%
3113910117010%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3910 - Cross-commodity research--multiple animal species;

Field Of Science
1101 - Virology; 1160 - Pathology; 1100 - Bacteriology; 1110 - Parasitology; 1150 - Toxicology; 1170 - Epidemiology;
Goals / Objectives
Goals and objectives are to: 1) identify and characterize new, emerging, or re-emerging spontaneous diseases in domestic animals and wildlife, including those potentially impacting human health; 2) determine the cause and pathogenesis of new, emerging, or re-emerging spontaneous diseases in wildlife and domestic animals; 3) document and track the incidence, prevalence and trends of spontaneous diseases as they occur state-wide; 4) validate commercial diagnostic tests or develop new tests for diseases in wildlife and domestic animals; similarly, validate or develop new assays for environmental toxins that have the potential to adversely affect animal or human health; and 5) leverage preliminary data from real-world cases of spontaneous disease or toxins in animals and wildlife or studies to generate extramural grants to support applied and basic research. Attainment of these goals and objectives will increase the knowledge about new, emerging, or re-emerging diseases in animals. This knowledge will help prevent or mitigate the effects of disease and toxins in animals, humans, and the environment. Knowledge acquired will be integrated into teaching efforts and disseminated to stakeholders through scientific and lay meetings publications, and press.
Project Methods
Case submissions to the WSVL are varied but the approach to diagnosis and characterization of new, emerging, or re-emerging diseases typically involves some combination of pathology, toxicology, virology, bacteriology, parasitology, and epidemiology/public health, as well as clinical pathology, molecular diagnostics, and electron microscopy. WSVL's philosophy is to identify and target unusual occurrences of animal disease, i.e., those with: 1) high unexplained morbidity or mortality, 2) an unusual constellation of clinical signs and lesions, 3) unique features that require additional testing beyond initial work-ups, or 4) an association with human illness. Depending on the nature and severity of the health problem, diagnosticians may go to the field to collect additional samples and case history. After initial characterization of the disease in individual animals, epidemiology can be used to put the disease occurrence into perspective, accounting for animal populations or animal/human interaction. As the investigation evolves, other health professionals from the Wyoming Department of Health, Wyoming Livestock Board, Wyoming Game and Fish Department, or federal agencies may be involved as the situation dictates. Outcomes of these disease investigations lead to better characterization and clinical recognition of diseases, development of new diagnostic tests and procedures, and insights about potential preventative or mitigation strategies.Most of the proposed studies utilize diagnostic submissions from naturally occurring disease events and do not require IACUC approval. In the event that additional non-diagnostic sampling is required, specific protocols will be submitted to the IACUC for approval prior to initiation of sampling.

Progress 12/08/15 to 09/30/20

Outputs
Target Audience:Efforts supported by this project affected livestock producers, veterinarians, regulatory agencies, public health officials and animal owners throughout the state of Wyoming and the region. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?In each of the accomplishments above, numerous graduate and undergraduate students participated in the studies. How have the results been disseminated to communities of interest?The results of work under this project have been presented to state and regional stakeholders at Wyoming Veterinary Medical Association meetings, the Wyoming Stockgrowers Association meeting, working group meetings including those with state and regional APHIS staff, the Wyoming Livestock Board and others. Direct contact with veterinarians and producers on specific problems occurs daily. In addition, this and related work is presented at national and international scientific meetings. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? The following is a summary of accomplishments by objective and disease/infection for this project. 1) identify and characterize new, emerging, or re-emerging spontaneous diseases in domestic animals and wildlife, including those potentially impacting human health; Histophilus somni Myocarditis in cattle infected with Histophilus somni is an increasingly recognized problem in feedlot cattle. Studies continued to characterize this disease and begin to develop control and prevention strategies. Mycoplasma bovis Mycoplasma bovis was identified as a cause of major fatal outbreaks of disease in free ranging pronghorn. This is the first recognition of this pathogen as a cause of disease in pronghorn. 2) determine the cause and pathogenesis of new, emerging, or re-emerging spontaneous diseases in wildlife and domestic animals; Cervid adenovirus Whole genome analysis of adenovirus isolates from a variety of wildlife defines a new species of adenovirus. This virus has been responsible for large die-offs of mule- and black-tailed deer. Based on this work the ICTV assigned this a new species in the genus Atadenovirus, with designation Deer atadenovirus A. This work not only resulted in the declaration of a new species of adenovirus but serves as the basis for development of very specific diagnostic and epidemiologic methods. Cervid herpesvirus Studies of keratoconjunctivitis in mule deer lead to the identification of a novel alphaherpesvirus. This virus will be included for designation as a new species in this year's ICTV proposal. Cryptosporidium parvum Cryptosporidium parvum was shown to be a previously unrecognized cause of infections in falcons. A major meta-analysis of Cryptosporidium infection in livestock and other ungulates was completed. SDCA2 Identification of the genetic basis for a novel neurodegenerative disease in Belgian sheepdogs, Spongy Degeneration with Cerebellar Ataxia (SDCA2). This is a rare familial disease which involved some cases from the WSVL. Identification of the SINE insertion in ATP1B2 responsible will facilitate testing to eradicate this genetic locus from the breed pool. 3) document and track the incidence, prevalence and trends of spontaneous diseases as they occur statewide; Chronic Wasting Disease Publication of field data indicating the severe effects of chronic wasting disease on white tailed deer population levels. This extensive longitudinal study using capture-recapture techniques on naturally occurring disease in wild populations provides considerable basis for planning and management of CWD in white tailed deer. Effect of endemic chronic wasting disease severe on mule deer population levels. This extensive longitudinal study using capture-recapture techniques on naturally occurring disease in wild populations provides considerable basis for planning and management of CWD in mule deer. The distribution of abnormal prion within the tonsils of CWD infected elk and mule deer were described. Toxoplasmosis Potential interaction between infection with Toxoplasma gondii and the resulting immune response, with the Huntington's disease locus in mice. Related work extends previous findings of nutritional effects on the progression of Huntington's disease. Brucella abortus Risk assessment for brucellosis in the Greater Yellowstone Area provided both risk models for wildlife-bovine transmission and cost-benefit analysis of possible approaches to control strategies for this disease in its last enzootic focus in the U.S. The impact of CWD on brucellosis control efforts in the Greater Yellowstone Area was evaluated. Brucella ovis The seroprevalence and risk factors for ovine brucellosis were determined for the state of Wyoming. The use of serologic testing of rams for management of Brucella ovis infections in domestic sheep flocks was evaluated. OPP An estimate of the prevalence of ovine progressive pneumonia and analysis of risk factors for this important disease were determined for Wyoming sheep flocks. 4) validate commercial diagnostic tests or develop new tests for diseases in wildlife and domestic animals; similarly, validate or develop new assays for environmental toxins that have the potential to adversely affect animal or human health Malignant Catarrhal Fever In collaboration with colleagues from UC Davis and others a new in situ hybridization method was developed for diagnosis in formalin fixed tissues and was used to further understand the pathogenesis of this disease through localization of viral nucleic acid in infected animals. Brucellosis Work on development of an effective PCR test for Brucella spp. lead to development and optimization of sampling enrichment and extraction protocols for this bacteria from tissues and blood. Validation of these techniques continues on field samples. Two serologic ELISA tests for detection of Brucella ovis infection were evaluated. Bibersteinia trehalosi A new real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi was developed to help identify virulent isolates of this bacterium. Toxoplasma gondii A new fluorescent bead-based serologic test for antibodies to Toxoplasma gondii in chickens was developed.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Central nervous signs, blindness and cerebral vermicosis in free-ranging peregrine falcons (Falco peregrinus) associated with aberrant larval migrations. Fischer D, Oberl�nder B, Peters M, Eley N, Pantchev N, Bangoura B, Lierz M. Vet Parasitol Reg Stud Reports. 2020 Apr;20:100410.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Rickettsia hoogstraalii and a Rickettsiella from the Bat Tick Argas transgariepinus, in Namibia. Reeves WK, Mans BJ, Durden LA, Miller MM, Gratton EM, Laverty TM. J Parasitol. 2020 Oct 1;106(5):663-669.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Mycoplasma bovis Infections in Free-Ranging Pronghorn, Wyoming, USA. Malmberg JL, O'Toole D, Creekmore T, Peckham E, Killion H, Vance M, Ashley R, Johnson M, Anderson C, Vasquez M, Sandidge D, Mildenberger J, Hull N, Bradway D, Cornish T, Register KB, Sondgeroth KS. Emerg Infect Dis. 2020 Dec;26(12):2807-2814.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Intoxication by Astragalus garbancillo var. garbancillo in llamas. Marin RE, Micheloud JF, Vignale ND, Gimeno EJ, O'Toole D, Gardner DR, Woods L, Uzal FA. J Vet Diagn Invest. 2020 May;32(3):467-470.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Comparison of 2 ELISAs for detecting exposure to Brucella ovis. Elderbrook MJ, Schumaker BA, Ueti MW, Bastos de Almeida M, Vieira TSWJ, Vieira RFC, Sondgeroth KS. J Vet Diagn Invest. 2020 Sep;32(5):700-705.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Chronic wasting disease undermines efforts to control the spread of brucellosis in the Greater Yellowstone Ecosystem. Maloney M, Merkle JA, Aadland D, Peck D, Horan RD, Monteith KL, Winslow T, Logan J, Finnoff D, Sims C, Schumaker B. Ecol Appl. 2020 Sep;30(6):e02129.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: City sanitation and socioeconomics predict rat zoonotic infection across diverse neighbourhoods. Murray MH, Fidino M, Fyffe R, Byers KA, Pettengill JB, Sondgeroth KS, Killion H, Magle SB, Rios MJ, Ortinau N, Santymire RM. Zoonoses Public Health. 2020 Sep;67(6):673-683.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Ruminant Coccidiosis. Bangoura B, Bardsley KD. Vet Clin North Am Food Anim Pract. 2020 Mar;36(1):187-203.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens. Fabian BT, Hedar F, Koethe M, Bangoura B, Maksimov P, Conraths FJ, Villena I, Aubert D, Seeber F, Schares G. Parasit Vectors. 2020 Jul 31;13(1):388.


Progress 10/01/18 to 09/30/19

Outputs
Target Audience:Efforts supported by this project affected livestock producers, veterinarians, regulatory agencies, public health officials and animal owners throughout the state of Wyoming and the region. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Numerous graduate and undergraduate students were involved in various aspects of these projects. How have the results been disseminated to communities of interest?The results of work under this project have been presented to state and regional stakeholders at Wyoming Veterinary Medical Association meetings, the Wyoming Stockgrowers Association meeting, working group meetings including those with state and regional APHIS staff, the Wyoming Livestock Board and others. Direct contact with veterinarians and producers on specific problems occurs daily. In addition, this and related work is presented at national and international scientific meetings. What do you plan to do during the next reporting period to accomplish the goals?The Department of Veterinary Sciences and the Wyoming State Vet Lab will continue to provide diagnostic and surveillance support to the state of Wyoming and the region. Case and other material resulting from this work will be analyzed and characterized to provide new knowledge and insights into the pathogenesis, immunity and behavior of pathogens in animal populations.

Impacts
What was accomplished under these goals? 1. Histophilus somni continues to be an increasingly recognized problem in feedlot cattle. Studies continued in 2019 to characterize this disease and begin to develop control and prevention strategies. 2. Studies from previous years of prion distribution in the tonsils of naturally infected mule deer and elk were published. 3. A broad review and meta-analysis of cryptosporidium in livestock was completed and published. 4. An in situ hybridization method for the detection of ovine herpesvirus 2, the causative agent of malignant catharral fever, in fixed tissues was developed. 5. Several grants were submitted based on results from studies performed in part under this project.

Publications

  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Margineda CA, O'Toole D, Prieto M, Uzal FA, Zielinski GC. Histophilus somni myocarditis and leptomeningitis in feedlot cattle: case report and occurrence in South America. J Vet Diagn Invest. 2019 Nov;31(6):893-898.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Malmberg JL, Lee JS, Gagne RB, Kraberger S, Kechejian S, Roelke M, McBride R, Onorato D, Cunningham M, Crooks KR, VandeWoude S. Altered lentiviral infection dynamics follow genetic rescue of the Florida panther. Proc Biol Sci. 2019 Oct 23;286(1913):20191689.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Gomes-Solecki M, Arnaboldi PM, Backenson PB, Benach JL, Cooper CL, Dattwyler RJ, Diuk-Wasser M, Fikrig E, Hovius JW, Laegreid W, Lundberg U, Marconi RT, Marques AR, Molloy P, Narasimhan S, Pal U, Pedra JHF, Plotkin S, Rock DL, Rosa P,Telford SR, Tsao J, Yang XF, Schutzer SE. Protective Immunity and New Vaccines for Lyme Disease. Clin Infect Dis. 2019 Oct 17. pii: ciz872.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Hille MM, Jewell JE, Belden EL. Cellular distribution of the prion protein in palatine tonsils of mule deer (Odocoileus hemionus) and Rocky Mountain elk (Cervus elaphus nelsoni). J Vet Med Sci. 2019 Nov 14;81(11):1586-1596.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Hatam-Nahavandi K, Ahmadpour E, Carmena D, Spotin A, Bangoura B, Xiao L. Cryptosporidium infections in terrestrial ungulates with focus on livestock: a systematic review and meta-analysis. Parasit Vectors. 2019 Sep 14;12(1):453.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Ahmadpour E, Ghanizadegan MA, Razavi A, Kangari M, Seyfi R, Shahdust M, Yazdanian A, Safarpour H, Bannazadeh Baghi H, Zarean M, Hosseini SA, Norouzi R, Ebrahimi M, Bangoura B. Strongyloides stercoralis infection in human immunodeficiency virus-infected patients and related risk factors: A systematic review and meta-analysis. Transbound Emerg Dis. 2019 Nov;66(6):2233-2243.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Elderbrook M, Schumaker B, Cornish T, Peck D, Sondgeroth K. Seroprevalence and risk factors of Brucella ovis in domestic sheep in Wyoming, USA. BMC Vet Res.2019 Jul 15;15(1):246. doi: 10.1186/s12917-019-1995-5.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Kechejian SR, Dannemiller N, Kraberger S, Ledesma-Feliciano C, Malmberg J, Roelke Parker M, Cunningham M, McBride R, Riley SPD, Vickers WT, Logan K, Alldredge M, Crooks K, L�chelt M, Carver S, VandeWoude S. Feline Foamy Virus is Highly Prevalent in Free-Ranging Puma concolor from Colorado, Florida and Southern California. Viruses. 2019 Apr 19;11(4). pii: E359.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Ivanova DL, Denton SL, Fettel KD, Sondgeroth KS, Munoz Gutierrez J, Bangoura B, Dunay IR, Gigley JP. Innate Lymphoid Cells in Protection, Pathology, and Adaptive Immunity During Apicomplexan Infection. Front Immunol. 2019 Feb 28;10:196.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Thabet A, Schm�schke R, Fertey J, Bangoura B, Sch�nfelder J, Lendner M, Ulbert S, Daugschies A. Eimeria tenella oocysts attenuated by low energy electron irradiation (LEEI) induce protection against challenge infection in chickens. Vet Parasitol. 2019 Feb;266:18-26.
  • Type: Journal Articles Status: Published Year Published: 2019 Citation: Branscom LA, Cornish TE, Sondgeroth KS. Evaluation of serologic testing of rams in the management of Brucella ovis in a domestic sheep flock. J Vet Diagn Invest. 2019 Jan;31(1):86-89.


Progress 10/01/17 to 09/30/18

Outputs
Target Audience:Efforts supported by this project affected livestock producers, veterinarians, regulatory agencies, public health officials and animal owners throughout the state of Wyoming and the region. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Numerous graduate and undergraduate students were involved in various aspects of these projects. How have the results been disseminated to communities of interest?The results of work under this project have been presented to state and regional stakeholders at Wyoming Veterinary Medical Association meetings, the Wyoming Stockgrowers Association meeting, working group meetings including those with state and regional APHIS staff, the Wyoming Livestock Board and others. Direct contact with veterinarians and producers on specific problems occurs daily. In addition, this and related work is presented at national and international scientific meetings. What do you plan to do during the next reporting period to accomplish the goals?The Department of Veterinary Sciences and the WSVL will continue to provide diagnostic and surveillance support to the state of Wyoming and the region. Case and other material resulting from this work will be analyzed and characterized to provide new knowledge and insights into the pathogenesis, immunity and behavior of pathogens in animal populations.

Impacts
What was accomplished under these goals? 1) Malignant catarrhal fever (MCF) is an important disease of cattle, bison and wild ungulates. In collaboration with colleagues from UC Davis and others a new in situ hybridization method was developed for diagnosis in formalin fixed tissues and was used to further understand the pathogenesis of this disease through localization of viral nucleic acid in infected animals. Cryptosporidium parvum was shown to be a previously unrecognized cause of infections in falcons. 2) Further studies on the impact of cervid adenovirus on mule deerwere completed. In addition, previous work from this lab indicated that the virus classification was incorrect and that this was in fact a new adenoviral type. The ICTV concurred this year and has assigned this a new species in the genus Atadenovirus, with designation Deer atadenovirus A. Studies of keratoconjunctivitis in mule deer lead to the identification of a novel alphaherpesvirus. This virus will be included for designation as a new species in this year's ICTV proposal. 3) Studies on the extent of Brucella ovis within Wyoming sheep flocks continue. This year we completed work on evaluation of serologic testing for B.ovis as a management tool for this economically important infectious disease. 4) Work on development of an effective PCR test for Brucella spp. lead to development and optimization of sampling enrichment and extraction protocols for this bacteria from tissues and blood. Validation of these techniques continues on field samples. A newreal-time PCR specific for the leukotoxin gene ofBibersteinia trehalosi was developed to help identify virulent isolates of this bacterium. 5) Several extramural funding proposals based on the above results were submitted.

Publications

  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Pesavento PA, Cunha CW, Li H, Jackson K, O'Toole D. In Situ Hybridization for Localization of Ovine Herpesvirus 2, the Agent of Sheep-Associated Malignant Catarrhal Fever, in Formalin-Fixed Tissues. Vet Pathol. 2018 Sep 16:300985818798085. doi: 10.1177/0300985818798085. [Epub ahead of print] PubMed PMID: 30222071
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Hull NC, Schumaker BA. Comparisons of brucellosis between human and veterinary medicine. Infect Ecol Epidemiol. 2018 Jul 24;8(1):1500846. doi: 10.1080/20008686.2018.1500846. eCollection 2018. Review. PubMed PMID: 30083304; PubMed Central PMCID: PMC6063340.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Mu�oz Guti�rrez JF, Sondgeroth KS, Williams ES, Montgomery DL, Creekmore TE, Miller MM. Infectious keratoconjunctivitis in free-ranging mule deer in Wyoming: a retrospective study and identification of a novel alphaherpesvirus. J Vet Diagn Invest. 2018 Sep;30(5):663-670. doi: 10.1177/1040638718787862. Epub 2018 Jul 21. PubMed PMID: 30032722
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Killion HJ, Edwards W, Jennings-Gaines J, Wood M, Fox K, Sondgeroth K. Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi. J Vet Diagn Invest. 2018 Jul;30(4):589-592. doi: 10.1177/1040638717753497. Epub 2018 Mar 30. PubMed PMID: 29601780.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Woods LW, Schumaker BA, Pesavento PA, Crossley BM, Swift PK. Adenoviral hemorrhagic disease in California mule deer, 1990-2014. J Vet Diagn Invest. 2018 Jul;30(4):530-537. doi: 10.1177/1040638718766036. Epub 2018 Mar 27. PubMed PMID: 29582709.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Azmanis P, di Somma A, Pappalardo L, Silvanose CD, Bangoura B. First detection of Cryptosporidium parvum in falcons (Falconiformes): Diagnosis, molecular sequencing, therapeutic trial and epidemiological assessment of a possible emerging disease in captive falcons. Vet Parasitol. 2018 Mar 15;252:167-172. doi: 10.1016/j.vetpar.2018.02.012. Epub 2018 Feb 9. PubMed PMID: 29559142.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Hull N, Miller J, Berry D, Laegreid W, Smith A, Klinghagen C, Schumaker B. Optimization of Brucella abortus Protocols for Downstream Molecular Applications. J Clin Microbiol. 2018 Mar 26;56(4). pii: e01894-17. doi: 10.1128/JCM.01894-17. Print 2018 Apr. PubMed PMID: 29436425; PubMed Central PMCID: PMC5869842.
  • Type: Journal Articles Status: Published Year Published: 2018 Citation: Branscom LA, Cornish TE, Sondgeroth KS. Evaluation of serologic testing of rams in the management of Brucella ovis in a domestic sheep flock. J Vet Diagn Invest. 2018 Dec 12:1040638718814591. doi: 10.1177/1040638718814591. [Epub ahead of print] PubMed PMID: 30541385.


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:Efforts supported by this project affected livestock producers, veterinarians, regulatory agencies, public health officials and animal owners throughout the state of Wyoming and the region. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?In each of the accomplishments above, numerous graduate and undergraduate students participated in the studies. How have the results been disseminated to communities of interest?All of the accomplishments listed above are published in high quality peer-reviewed journals. In addition, all have been presented at scientific and professional meetings. The CWD results were the basis for a number of popular press resports. What do you plan to do during the next reporting period to accomplish the goals?Further studies are underway on CWD in wild deerand brucellosis control in elk.

Impacts
What was accomplished under these goals? 1.Effect of endemic chronic wasting diseasesevere on mule deer population levels. This extensive longitudinal study using capture-recapture techniques on naturally occurring disease in wild populations provides considerable basis for planning and management of CWD in mule deer. 2. Whole genomeanalysis of adenovirus isolates from a variety of wildlife defines a new species of adenovirus. This virus has been responsible for large die-offs of mule and black tailed deer. This study not only resulted in the declaration of a new species of adenovirus but serves as the basis for developmentof very specific diagnostic and epidemiologic methods. 3. Identification of the genetic basis for a novel neurodegenerative disease in belgian sheepdogs. This is a rare familial disease which involved some cases from the WSVL. Identification of the SINE insertion responsible will facilitate testing to eradicate this genetic locus from the breed pool.

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Miller MM, Cornish TE, Creekmore TE, Fox K, Laegreid W, McKenna J, Vasquez M, Woods LW. Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus. J Gen Virol. 2017 Sep;98(9):2320-2328.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Bradshaw AC, Tell LA, Ernest HB, Bahan S, Carlson J, Sehgal RNM. Detection and prevalence of Haemoproteus archilochus (Haemosporida, Haemoproteidae) in two species of California hummingbirds. Parasitol Res. 2017 Jul;116(7):1879-1885.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Mauri N, Kleiter M, Dietschi E, Leschnik M, H�gler S, Wiedmer M, Dietrich J, Henke D, Steffen F, Schuller S, Gurtner C, Stokar-Regenscheit N, O'Toole D, Bilzer T, Herden C, Oevermann A, Jagannathan V, Leeb T. A SINE Insertion in ATP1B2 in Belgian Shepherd Dogs Affected by Spongy Degeneration with Cerebellar Ataxia (SDCA2). G3 (Bethesda). 2017 Aug 7;7(8):2729-2737.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: DeVivo MT, Edmunds DR, Kauffman MJ, Schumaker BA, Binfet J, Kreeger TJ, Richards BJ, Sch�tzl HM, Cornish TE. Endemic chronic wasting disease causes mule deer population decline in Wyoming. PLoS One. 2017 Oct 19;12(10):e0186512.


Progress 12/08/15 to 09/30/16

Outputs
Target Audience:Efforts supported by this project affectedlivestock producers, veterinarians, regulatory agencies, public health officials and animal owners throughout the state of Wyoming and the region. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?In each of the accompishments listed for the reporting period, numerous graduate and undergraduate students participated in the studies. How have the results been disseminated to communities of interest?All of the accomplishments listed for the reporting period are published in high quality peer-reviewed journals. In addition, all have been presented at scientific and professional meetings. The brucellosis risk and cost-benefit data were presented at a series of producer group meetings in the GYA and associated areas. What do you plan to do during the next reporting period to accomplish the goals?Further studies are underway on CWD in wild deer, bluetongue virus transmission to cattle and brucellosis control in elk.

Impacts
What was accomplished under these goals? Most of the major accomplishmentsin 2015-16 are related to objective 3,document and track the incidence, prevalence and trends of spontaneous diseases as they occur state-wide. The first of these is the publication of field data indicating the severe effects of chronic wasting disease on white tailed deer population levels. This extensive longitudinal study using capture-recapture techniques on naturally occurring disease in wild populations provides considerable basis for planning and management of CWD in white tailed deer. Another significant publication finds potential interaction between infection with Toxoplasma gondii and the resulting immune response, with the Huntington's disease locus in mice. Related work extends previous findings of nutritional effects on the progression of Huntington's disease. Two papers on risk assessment for brucellosis in the Greater Yellowstone Area provide both risk models for wildlife-bovine transmission and cost-benefit analysis of possible approaches to control strategies for this disease in its last enzootic focus in the U.S.Finally, a current estimate of the prevalence of ovine progressive pneumonia and analysis of risk factors for this important disease were determined for Wyoming sheep flocks.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Edmunds DR, Kauffman MJ, Schumaker BA, Lindzey FG, Cook WE, Kreeger TJ, Grogan RG, Cornish TE. Chronic Wasting Disease Drives Population Decline of White-Tailed Deer. PLoS One. 2016 Aug 30;11(8):e0161127.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Donley DW, Olson AR, Raisbeck MF, Fox JH, Gigley JP. Huntingtons Disease Mice Infected with Toxoplasma gondii Demonstrate Early Kynurenine Pathway Activation, Altered CD8+ T-Cell Responses, and Premature Mortality. PLoS One. 2016 Sep 9;11(9):e0162404.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Berggren KL, Lu Z, Fox JA, Dudenhoeffer M, Agrawal S, Fox JH. Neonatal Iron Supplementation Induces Striatal Atrophy in Female YAC128 Huntington's Disease Mice. J Huntingtons Dis. 2016;5(1):53-63.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Fox J, Lu Z, Barrows L. Thiol-disulfide Oxidoreductases TRX1 and TMX3 Decrease Neuronal Atrophy in a Lentiviral Mouse Model of Huntington's Disease. PLoS Curr. 2015 Nov 6;7. pii: ecurrents.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Speiser KL, Schumaker BA, Cook WE, Cornish TE, Cammack KM, Miller MM. Comparison of the humoral response between sheep vaccinated with a killed-virus vaccine and those vaccinated with a modified-live virus vaccine against bluetongue virus serotype 17. J Am Vet Med Assoc. 2016 May 1;248(9):1043-9.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Boroff K, Kauffman M, Peck D, Maichak E, Scurlock B, Schumaker B. Risk assessment and management of brucellosis in the southern greater Yellowstone area (II): Cost-benefit analysis of reducing elk brucellosis prevalence. Prev Vet Med. 2016 Nov 1;134:39-48.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Kauffman M, Peck D, Scurlock B, Logan J, Robinson T, Cook W, Boroff K, Schumaker B. Risk assessment and management of brucellosis in the southern greater Yellowstone area (I): A citizen-science based risk model for bovine brucellosis transmission from elk to cattle. Prev Vet Med. 2016 Sep 15;132:88-97.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Gerstner S, Adamovicz JJ, Duncan JV, Laegreid WW, Marshall KL, Logan JR, Schumaker BA. Prevalence of and risk factors associated with ovine progressive pneumonia in Wyoming sheep flocks. J Am Vet Med Assoc. 2015 Oct 15;247(8):932-7.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Vu HL, Ma F, Laegreid WW, Pattnaik AK, Steffen D, Doster AR, Osorio FA. A Synthetic Porcine Reproductive and Respiratory Syndrome Virus Strain Confers Unprecedented Levels of Heterologous Protection. J Virol. 2015 Dec;89(23):12070-83.