Source: UNIVERSITY OF WYOMING submitted to
EFFECTS OF LIMITED IRRIGATION ON HERBICIDE EFFICACY AND HERBICIDE CARRY OVER
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
1005893
Grant No.
(N/A)
Project No.
WYO-552-15
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Apr 1, 2015
Project End Date
Sep 30, 2018
Grant Year
(N/A)
Project Director
Kniss, AN.
Recipient Organization
UNIVERSITY OF WYOMING
1000 E UNIVERSITY AVE DEPARTMENT 3434
LARAMIE,WY 82071-2000
Performing Department
Plant Sciences
Non Technical Summary
The study will evaluate the impacts of limited irrigation on soil-applied herbicide efficacy and break down. Soil applied herbicides used in dry beans, and corn will be tested under three moisture levels to determine the effects on herbicide efficacy and potential carryover to sugarbeet, dry beans, barley and corn. These crops are all frequent components of crop rotations in the Big Horn Basin of Wyoming. In addition, indoor bioassays will be compared to field bioassays for their ability to predict potential crop injury.
Animal Health Component
0%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
11101201140100%
Knowledge Area
111 - Conservation and Efficient Use of Water;

Subject Of Investigation
0120 - Land;

Field Of Science
1140 - Weed science;
Goals / Objectives
Determine the impact of limited irrigation on efficacy of soil-applied herbicides that are commonly used in corn and dry-bean production.Limited irrigation may reduce weed control from soil-applied herbicides early in the season, which may increase reliance on post emergence herbicide use.By reducing microbial breakdown, limited irrigation may increase late-season herbicide availability, improving late-season weed control.Determine the impact of limited irrigation on herbicide dissipation in the soil and potential carryover to rotational crops.By reducing microbial breakdown of the herbicide, limited irrigation may result in increased availability of the herbicide the following year, causing potential yield loss in rotational crops.It is possible that a fall soil bioassay may predict crop yield loss potential or the following spring under limited irrigation conditions.
Project Methods
The study will be conducted at the Wyoming Agricultural Experiment Station Powell Research and Extension Center (PREC). The experimental design will be a randomized complete block with three replications, with irrigation levels, crops and herbicides as factors. In the spring of 2015, corn and dry beans will be planted in twenty-two inches rows and grown under lateral sprinkler irrigation. This system will allow applying three irrigation treatments, consisting of 100, 85 and 70% of the crops water needs. Plots will consist of 12 rows with an approximate size of 22 by 35 ft. Herbicides will be applied at the recommended rates with a back sprayer. Herbicides in corn will include atrazine, verdict, zidua and prequel; herbidcides in dry beans will include pursuit, prowl, sonalan, and treflan. Herbicide efficacy will be evaluated by counting the number of each individual weed species in a 1m2 area, for a total of four weed counts per plot. The counts to establish herbicide efficacy will be completed at three different times during the growing season: at crop emergence, 15 days after the application, and before crop harvest. After-harvest crop residue will be incorporated into the soil. Early in spring of 2016 soil will be sampled at two different depths (0" to 3" and 3" to 6") to determine the amounts of herbicides remaining in soil approximately a year after application. Part of the soil sample will be sent to a laboratory for a quantitative determination of the herbicide remaining in the soil, and an indoor bioassay will be conducted with the rest of the soil sample. Bioassays are a relatively inexpensive way to determine crop injury due to herbicide residues in soil, when compared to lab testing. The bioassay will be conducted in the greenhouse facility located at PREC in spring of 2016 and 2017 soon after the collection of soil samples. Samples from each plot will be placed in 9" x 18" plastic trays and corn, dry bean, sugarbeet or barley will be planted depending on previous crops. Visual evaluations of herbicide injury will be conducted at five day intervals for a period of two weeks. Plants will be harvested, oven dried and weighed to determine plant biomass.Unless crop injury is very high, it is difficult to predict how the injury observed in the indoor bioassay correlates to potential crop yield reduction. Therefore, in order to overcome this limitation a field bioassay will be conducted in spring of 2016. Areas planted to corn and dry beans and treated with different soil applied herbicides the previous year will be planted to different crops to assess the effects of herbicide residues under field conditions. Plots from the year 2015 will be divided in three and planted perpendicularly to dry beans, corn, sugarbeet or barley depending on previous crop. The plot size for the second year of the study will be 11 by 22 ft. Crops will be visually evaluated after crop emergence for herbicide injury and harvested later in the season to determine yields. A limitation of the study is dictated by the fact that herbicide breakdown is also influenced by weather conditions, and unusual weather conditions during the course of the study can't be predicted. In order to overcome this limitation a repetition of the study (greenhouse bioassay included) will begin in 2016 in an adjacent field, extending the total duration of the project to three years.Perhaps the major limitation of the study is the fact that tillage, a factor affecting herbicide break down and carryover will not be tested in this study due to restrictions in available space for conducting the study and available funding.

Progress 04/01/15 to 09/30/18

Outputs
Target Audience: Nothing Reported Changes/Problems:The primary PI died before the conslusion of the study, so there was a major delay in completing some of the writing and analysis. This has now been finished, and a draft of the manuscript has been completed, and will be submitted soon. What opportunities for training and professional development has the project provided?1 M.S. degree was awarded with this study as the thesis project. How have the results been disseminated to communities of interest?1 peer-reviewed article is in preparation. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Soil-applied herbicides are important for controlling weeds in many crops, as they offer a broadened control spectrum and chemical diversity when POST-applied herbicide options are limited. However, if soil-applied herbicides persist for an extended time, there is risk for damage to susceptible rotational crops in succeeding years. As herbicide degradation in the soil is dependent on water, among other factors, needs to reduce agricultural water use in the future could lead to limited herbicide degradation and a greater risk for carryover. This project seeks to understand how limited irrigation affects dissipation of soil-applied herbicides in irrigated crop rotations. A field study was undertaken by applying 8 soil-applied herbicides to dry bean and corn. Three irrigation treatments (100, 85, and 70% of crop evapotranspiration) were applied with an overhead sprinkler. Soil samples taken 0, 1, 7, 14, 21, 28, 42, 56, 70, 84, 112, and 140 d after application were analyzed for herbicide level using gas or liquid chromatography and mass spectrometry. Results were regressed over time to produce a degradation curve and soil half-life estimate for each herbicide and irrigation treatment. Reduced irrigation never significantly increased soil half-life of any herbicide tested for both study years. Growth and yield of rotational crops planted one year following herbicide application supported these results, as reduced irrigation did not increase the risk of carryover. Instead, carryover was primarily determined by the inherent persistence of individual herbicides. Imazethapyr (0.1 kg ai ha-1) injured rotational sugar beet, corn, and sunflower. Isoxaflutole (0.1 kg ai ha-1) injured rotational dry bean and sunflower. Pyroxasulfone (0.2 kg ai ha-1) injured rotational sugar beet in one of two years. Atrazine (2.0 kg ai ha-1), saflufenacil (0.1 kg ai ha-1) + dimethenamid-p (0.6 kg ai ha-1), ethalfluralin (0.8 kg ai ha-1), trifluralin (0.6 kg ai ha-1), and pendimethalin (1.1 kg ai ha-1) did not injure rotational crops.

Publications


    Progress 10/01/16 to 09/30/17

    Outputs
    Target Audience: Nothing Reported Changes/Problems:The originalPI for this project died in a tragic motorcycle accident. We are doing the best we can to bring this project to completion but things have fallen behind the original schedule. Dr. Sbatella left big shoes to fill. What opportunities for training and professional development has the project provided?An M.S. student completed his degree with this project as his thesis. How have the results been disseminated to communities of interest?Data has been presented to scientists at professional society meetings. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

    Impacts
    What was accomplished under these goals? Field study was finished in 2017, final data has been collected, but is still being analyzed.

    Publications

    • Type: Conference Papers and Presentations Status: Awaiting Publication Year Published: 2017 Citation: Adamson et al. (2017) Dissipation of Soil-Applied Herbicides under Limited Irrigation. Proc. Western Soc. Weed Sci. 129.
    • Type: Conference Papers and Presentations Status: Awaiting Publication Year Published: 2017 Citation: Adamson et al. (2017) Crop Response to Soil-Applied Herbicide Carryover. Proc Western Soc. Weed Sci. 37.


    Progress 10/01/15 to 09/30/16

    Outputs
    Target Audience:The study area was visited by college students and Ag professionals, in order to show the effects of herbicide carryover in rotational crops. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Grad student is learning the laboratory procedures for determining herbicide residues in soil. The study area was visited by college students and Ag professionals, in order to show the effects of herbicide carryover in rotational crops. How have the results been disseminated to communities of interest?Partial results have been shared to growers and interested public in field days, and to other prefessionals in professional meetings. What do you plan to do during the next reporting period to accomplish the goals?In 2017, the field bio-assay portion of the second study will be completed, and all soil samples will be processed to determine herbicide degradation.

    Impacts
    What was accomplished under these goals? The field study initiated in 2015, continued during the 2016 growing season at the Powell Research and Extension Center. During this season the portion of the study initiated the previous years was planted to sugarbeet, corn, sunflower or dry beans as a field bioassay, to evaluate the effects of herbicide carry over under different irrigation regimes. Crops were evaluated for injury during the growing season and harvested for yield determination. In an adjacent field, a second run of the study began in 2016, with same methodology used in 2015. In fall of 2016, all soil samples collected from both studies, are being process to determine herbicides levels remaining in soil. The objective is to determine degradation rates of the tested herbicides under different irrigation levels.

    Publications

    • Type: Other Status: Published Year Published: 2016 Citation: G. Sbatella and A. R. Kniss. 2016.Effects of Limited Irrigation on Herbicide Efficacy and Herbicide Carryover.Field Days Bulletin 2016, Wyoming Agricultural Experiment Station. Pg 75-76.


    Progress 04/01/15 to 09/30/15

    Outputs
    Target Audience:During the year 2015 a field study was initiated at the Powell Research and Extension Center where four different soil-applied herbicides were applied to dry beans and corn respectively. Crops were grown under three irrigation regimes (100, 80, and 69 % of crop evapostranspiration), and soil moisture was monitored using GS1 soil moisture sensors. Volumetric soil water content of the three irrigation treatments averaged 22%, 18% and 17% throughout the growing season. Crop yields decreased as irrigation was reduced. Soil samples were collected at regular intervals following herbicide application and will be analyzed in the lab in the year 2016 to determine herbicide levels remaining in the soil. A second set of soil samples was collected in fall of 2015 and will be used in 2016 to perform a greenhouse bioassay to determine crop response to residual herbicide. These results will be compared to crop response in the field during the 2016 growing season, when sugar beet, sunflower, and dry bean or corn will be planted over the original plots and assessed for herbicide damage. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Graduate students have become familair with soil-water content monitoring techniques, irrigation techniques, herbicide applications, data collection. How have the results been disseminated to communities of interest?Preliminary results from the first year have been presented by the graduate student at the University Wyoming and in professional meetings (Western Society of Weed Science). What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

    Impacts
    What was accomplished under these goals? Data was collected from the 2015 field study to compare with 2016 observations which will allow preliminary conclusions regarding the major goals for the project.

    Publications