Progress 02/15/15 to 02/14/17
Outputs
Target Audience:Use of the MiSeq and data generated have targeted scientists and educators in the field of microbiology and animal physiology. Additionally, graduate and undergraduate students were trained in techniques and knowledge of next generation sequencing. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Graduate students and undergraduate students were trained in cell culture, RNA extraction and library preparation and sequencing. Additionally, they were trained in data analysis and interpretation, manuscript preparation and presentation of data. How have the results been disseminated to communities of interest?Initiall findings were presented at the American Society of Animal Science meeting and are currently in preparation for manuscript submission. Findings were also presented to faculty, staff and students at the University of Connecticut. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
We have demonstrated that the host-pathogen interaction between Staphylococcus Aureus and bovine mammary epithelial cells alters gene expression in both the host and the pathogen. Additionally, these changes are associated with changes in cell function, viability and adhesion. Plant-derived antimicrobials reduced adhesion and invasion of S. Aureus to mammary epithelial cells in culture and these changes are associate with changes in cell adhesion gene expression in the pathogen. These findings are currently in preparation for publication.
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2015
Citation:
Valley, EV, Jaganathan, D, Venkitanarayanan, K, Kazmer, GW, Kuo, L, Wang, YB, and Govoni, KE. 2015. Effects of plant derived compounds on Staphylococcus aureus infection of primary bovine mammary epithelial cells. J. Anim. Sci. Vol. 93, Suppl. s3.
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Progress 02/15/15 to 02/14/16
Outputs
Target Audience:The experiments that will utlize the Illumina MiSeq Equipment target a scietific audience including scientists, faculty, postdocs, graduate students and undergraduates. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This equipment and the proposed exerperiments have provided training for graduate and postdocs in whole transcriptome sequencing analysis. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?Additional sequencing analysis will be completed using the MiSeq to meet the remaining objective in the proposal.
Impacts
What was accomplished under these goals?
Use of the Illumina Mi-Seq analyzer, we have sequenced the whole transcriptome of staphylococcus aureus in the absence and presence of plant-derived compounds trans-cinnemaldehyde (TC) and eugenol (EG). Treatment of SA with TC resulted in 110 differentially expressed genes of which 3 were down-regulated greater than 2-fold and 10 were up-regulated greater than 2-fold. Treatment of SA with EG resulted in 222 differentially expressed genes of which 17 were down-regulated by greater than 2-fold and 13 were up-regulated greater than 2-fold. To gain a better understanding of the host-pathogen interaction during mastitis infection, we developed an in vitro model in which MEC were infected with SA and then RNA was isolated from both the MEC and SA for whole transcriptome analysis. RNA-Seq analysis using the MiSeq determined that 706 genes in SA are differentially expressed when exposed to MEC. Currently analysis is in progress to identify the functions and pathways in which these genes are involved.
Publications
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