Source: UNIV OF WISCONSIN submitted to
NANOTECHNOLOGY AND BIOSENSORS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
1003629
Grant No.
(N/A)
Project No.
WIS01782
Proposal No.
(N/A)
Multistate No.
NC-_old1194
Program Code
(N/A)
Project Start Date
Oct 1, 2014
Project End Date
Sep 30, 2016
Grant Year
(N/A)
Project Director
Gunasekaran, S.
Recipient Organization
UNIV OF WISCONSIN
21 N PARK ST STE 6401
MADISON,WI 53715-1218
Performing Department
Biological Systems Engineering
Non Technical Summary
An easy-to-use biosensor will be developed to test food suspensions in out-of-lab settings for the presence or absence of target bacteria. The system will be optimized to detect various amounts of target organisms.
Animal Health Component
0%
Research Effort Categories
Basic
50%
Applied
30%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
71250102020100%
Knowledge Area
712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins;

Subject Of Investigation
5010 - Food;

Field Of Science
2020 - Engineering;
Goals / Objectives
Develop new technologies for characterizing fundamental nanoscale processes Develop devices and systems incorporating microfabrication and nanotechnology
Project Methods
We will use an immunogenic biosensing method. We will employ a bifunctional linkler that has the binding affinity to the target organism as well as to aggregate gold nanoparticles. The aggregation of gold nanoparticles, in the absence of target organism, will give raise to change in the system color -- from red to purple. Once optimized, the red-to-purple color change can be a visual indication of the absence of organism above a pre-set threshold level.

Progress 10/01/14 to 09/30/16

Outputs
Target Audience:The target audiences reached are academics and industry personnel. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?One graduate student is pursuing PhD. How have the results been disseminated to communities of interest?Both via conference paper presentations and peer-review research publications. What do you plan to do during the next reporting period to accomplish the goals?This work is continued under Hatch project 1010419, where we will continue to investigate the effect of system parameters such as size,shape, and concentration of gold nanoparticles on detection sensitivity.

Impacts
What was accomplished under these goals? We have developed a system for visible detection of microorganisms based on the surface plasmon resonance effect of aggregating gold nanoparticles. The aggregation of streptavidin-coated gold nanoparticles is mediated by biotin-labelled antibodies, which we call switchable linkers. Gold nanoparticles are poorly aggregated when the switchable linkers are attached to the target organisms. Hence, the presence of target organisms interferes with the extent of aggregation of gold nanoparticles. Thus, the resulting color change can be used to indicate the presence of target organism at or above a certain threshold value. We are using E. coli as target organism to test and optimize our system. The detection sensitivity achieved is less than 10 CFU/mL.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: You Y, S Lim, S Gunasekaran. 2015. Highly sensitive and visible detection of bacteria in water. IFT Annual Meeting, July 11-14, Chicago, IL.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Lim S, S Gunasekaran. 2014. In situ synthesis of gelatin-capped gold nanoparticles as food thermal history indicator. IEEE Nano/Micro Engineered and Molecular Systems (NEMS), April 13-16, Honolulu, HI.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Bagci, P, Wang, Y-C, Gunasekaran S. 2015. A green route for synthesis of gold nanoparticles and selective colorimetric detection of cysteine. J. Food Sci, N2071N2078
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Wang YC, Lu L, Gunasekaran S. 2015. Gold nanoparticle-based thermal history indicator for monitoring low-temperature storage. Microchimica Acta 182:1305-11


Progress 10/01/14 to 09/30/15

Outputs
Target Audience:The target audiences are academia and industry. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?A graduate student is beingsupported for hisPhD training. How have the results been disseminated to communities of interest?The results have been disseminated through conference presentations and in a peer-review journal article. What do you plan to do during the next reporting period to accomplish the goals?In the next reporting period, we will synthesize gold nanoparticles of different sizes and shapes to investigate their effect of pathogen detection effectiveness in terms of improved sensitivity and rapidity. We will also continue work by testing several indicator organisms in buffer and some real matrices.

Impacts
What was accomplished under these goals? A gold nanoparticles-based immunosensor is being designed and devloped. The surface plasmon resonance (SPR) effect when gold nanoparticles aggregate provides color change which is used as an indicator for the detection of the presence of target organism. The detection schme uses a bifunctional linker, which is labelled antibody. Antibody facilitatesbinding of target bacteria through antibody-antigen immunogenic reaction. Biotin labelling of the antibody leads to aggregation of streptavidin-coatedgold nanoparticles via the well-known streptavidin-biotin interaction. We expect highly sensitive and visible detcetion of target bacteria via this proposed scheme.

Publications