Progress 09/01/12 to 08/31/16
Outputs Target Audience:Target audiences of scientific presentations during the reporting period included plant pathologists, weed scientists, soil scientists, horticulturalists, students, agricultural professionals and consultants, extension specialists, and policy makers from the USA and other countries. Target audiences of extension and outreach presentations included farmers, extension personnel, students and agricultural consultants. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?In addition to outreach activities to growers and agricultural professionals (detailed below), this project funded one Ph.D.-level graduate student (2013 to 2016) and partial funding for one post-doctoral research associate, both of whom focused on research with anaerobic soil disinfestation in lab, growth chamber, and field settings. Four undergraduate students (at UT), four community college students (at USDA-ARS), two visiting scientists and one high school student were also engaged in ASD-specific training. How have the results been disseminated to communities of interest?Outreach during the reporting period included maintenance of a website to highlight research projects, research publications and technical publications. On-farm demonstrations includedonein Tennessee and threein Florida during the reporting period. Field day presentations during the reporting period included one in Tennessee to approximately 60 grower and public attendees, one producer field day in Florida (10 attendees) and two publicfield days in Florida (80 total attendees). What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts What was accomplished under these goals?
For Obj. 1, pot and field studies were conducted to evaluate multiple C:N ratios as well as total C input rates; two field trials were conducted to evaluate alternative nitrogen inputs combined with agricultural carbon sources. Trials included analysis of crop performance, tissue nutrient analysis, disease incidence and in field trials, weed control, nematode population dynamics, and organic acid generation. A Ph.D. student dissertation was completed in the research area and abstracts and manuscripts published and additional manuscripts are being submitted to refereed journals. For Obj. 2, experiments were completed on the detection of Salmonella with enrichment before and after ASD treatment. No Salmonella could be detected in any inputs prior to amendment, during the cropping season or in any plants or fruit, regardless of picking stage. For Obj. 3, Field trials were repeated on the suppression of disease with post-treatment applications. Greenhouse trials were conducted to further investigate post-application disease suppression. For Obj. 4, we continued to present results of these trials at scientific meetings and field days (see following sections).
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2016
Citation:
Hong, J.C., K.J. Martin, N. Kokalis-Burelle, D.M. Butler, J. Albano, and E.N. Rosskopf. 2016. Evaluation nitrogen amendments for anaerobic soil disinfestation. Proceedings of 5th International Symposium on Tomato Diseases. Malaga, Spain, 13-16 June 2016.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Rosskopf, E.N., J.C. Hong, D.M. Butler, U. Shrestha and K. Martin. 2015. The importance of biotechnology in the search for alternatives to soil fumigation. Biotechnology for Better Tomorrow. October 29-31, 2015, Tampa, FL, pp 3-4.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Rosskopf, E.N.*, J.C. Hong, N. Kokalis-Burelle, M. Ozores-Hampton, F. DiGioia, X. Zhao, Z. Black, Z. Gao, C. Wilson, J. Thomas, K. Monaghan, M. Swisher, H. Guo, J. Muramoto, C. Shennan, U. Shrestha and D.M. Butler. 2015. Addressing practical limitation to the adoption of anaerobic soil disinfestation. Proceedings of the Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions 11-1 to 11-2.
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Shrestha, U., R.M. Aug�, and D.M. Butler. 2016. A meta-analysis of the impact of anaerobic soil disinfestation on pest suppression and yield of horticultural crops. Frontiers in Plant Science 7:1254. doi: 10.3389/fpls.2016.01254.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2016
Citation:
Shrestha, U. 2016. Anaerobic soil disinfestation: meta-analysis and optimization of amendment carbon rate and C:N ratio to control key plant pathogens and weeds. University of Tennessee, Knoxville, Ph.D. Dissertation. http://trace.tennessee.edu/utk_graddiss/3963/.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2016
Citation:
Shrestha, U., R.M. Auge, A. Saxton and D.M. Butler. 2016. A Meta-analysis of efficacy of anaerobic soil disinfestation on soil borne pathogen suppression. 2016 APS Annual Meeting. 30 July 3 Aug, Tampa, FL.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Shrestha, U., B.H. Ownley, E.N. Rosskopf and D.M. Butler. 2015. Amendment carbon rate and C:N ratio impacts the effectiveness of anaerobic soil disinfestation. Proceedings of the Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions; 10-1 to 10-3.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2016
Citation:
Shrestha, U., M. Dee, B.H. Ownle and D.M. Butler. 2016. Effect of anaerobic soil disinfestation on survival of S. rolfsii sclerotia and soil populations of Trichoderma (Abstr.) Phytopathology 106:S2.6. doi: 10.1094 / PHYTO-106-4-S2.6
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Progress 09/01/14 to 08/31/15
Outputs Target Audience:Target audiences of scientific presentations during the reporting period included plant pathologists, weed scientists, soil scientists, horticulturalists, agricultural professionals and consultants, extension specialists, and policy makers from the USA and other countries. Target audiences of extension and outreach presentations included farmers, extension personnel, and agricultural consultants. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?In addition to outreach activities to growers and agricultural professionals (detailed below), this project funds one Ph.D.-level graduate student (began in Jan 2013) and partial funding for one post-doctoral research associate, both of whom are focused on research with anaerobic soil disinfestation in lab, growth chamber, and field settings. Six undergraduate students (at UT), four community college students (at USDA-ARS), two visiting scientists and one high school student have been engaged in ASD-specific training. How have the results been disseminated to communities of interest?Outreach during the reporting period included presentations at a second roundtable organized by the California Strawberry Commission and maintenance of a website to highlight research projects, research publications, technical publications, and videos (available at http://vegetables.tennessee.edu/asd). One hands-on demonstration has been conducted in Florida in cooperation with the Saint Lucie County Extension Service during this rating period as well as one public demonstration in cooperation with the Saint Lucie County School Board. Two on-farm demonstrations of ASD were conducted and one has been used as a public demonstration site. What do you plan to do during the next reporting period to accomplish the goals?For Obj. 1, analysis of data and preparation of manuscripts from pot and field studies will continue, with expectation that multiple publications will be submitted during the next reporting period. For Obj. 2, augmentation studies will be conducted in the spring. For Obj. 3, data analysis of field trials will be completed. For Obj. 4, outreach activities will continue through a variety of methods and venues, including technical and scientific publications, field days, additional on-farm studies, and scientific meetings.
Impacts What was accomplished under these goals?
For Obj. 1, pot studies were conducted to evaluate multiple C:N ratios as well as total C input rates; two field trials were conducted to evaluate alternative nitrogen inputs combined with agricultural carbon sources. Trials included analysis of crop performance, tissue nutrient analysis, disease incidence and in field trials, weed control, nematode population dynamics, and organic acid generation. For Obj. 2, experiments were completed on the detection of Salmonella with enrichment before and after ASD treatment. No Salmonella could be detected in any inputs prior to amendment, during the cropping season or in any plants or fruit, regardless of picking stage. For Obj. 3, field trials were repeated on the suppression of disease with post-treatment applications. Greenhouse trials were conducted to further investigate post-application disease suppression. For Obj. 4, we have continued to present results of these trials at scientific meetings and field days (see following sections).
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Hong, J.C., K.J. Martin, N. Kokalis-Burelle, D.M. Butler and E.N. Rosskopf. 2014. Soil bacterial populations identified in anaerobic soil disinfestation in Florida. Proceedings of the Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions; pp. 13-1.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Hong, J.C., K.J. Martin, N. Kokalis-Burelle, D.M. Butler, P. Serrano- P�rez and E.N. Rosskopf. 2015. Changes in microbial communities associated with anaerobic soil disinfestation. Annual Meeting of the American Phytopathological Society; Pasadena, CA, 1-5 Aug 2015.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Rosskopf, E.N., C. Shennan, N. Kokalis-Burelle, D.M. Butler, P. Serrano-P�rez, M.d.C. Rodr�quez-Molina and J.C. Hong. 2015. Application of anaerobic soil disinfestation in Florida: A brief review. Annual Meeting of the American Phytopathological Society; Pasadena, CA, 1-5 Aug 2015.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Shrestha, U., M.M. Dee, B.H. Ownley and D.M. Butler. 2015. Anaerobic soil disinfestation amendment carbon rate affects germination and parasitism on sclerotia of Sclerotium rolfsii. Annual Meeting of the American Phytopathological Society; Pasadena, CA, 1-5 Aug 2015.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Kokalis-Burelle, N., J. Hong, D.M. Butler and E.N. Rosskopf. 2015. Anaerobic soil disinfestation (ASD) combined with soil solarization for root-knot nematode control in vegetable and ornamental crops. Society of Nematologists 54th Annual Meeting Abstracts; East Lansing, MI, 19-24 Jul 2015.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
Hong, J.C., K.J. Martin, N. Kokalis-Burelle, D.M. Butler and E.N. Rosskopf. 2015. Comparison of commercially available soil DNA extraction kits on soil treated with anaerobic soil disinfestation. Phytobiomes 2015: Designing a New Paradigm for Crop Improvement; Washington, DC, 30 Jun-2 Jul 2015.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Shrestha, U., A. Bruce, B.H. Ownley and D.M. Butler. 2014. Optimizing amendment C:N ratio for Fusarium oxysporum f. sp. lycopersici suppression under anaerobic soil disinfestation. Proceedings of the Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions; pp. 53-1 to 53-3.
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Progress 09/01/13 to 08/31/14
Outputs Target Audience: Target audiences of scientific presentations during the reporting period included plant pathologists, weed scientists, soil scientists, horticulturalists, agricultural professionals and consultants, extension specialists, and policy makers from the USA and other countries. Target audiences of extension and outreach presentations included farmers, extension personnel, and agricultural consultants. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? In addition to outreach activities to growers and agricultural professionals (detailed below), this project funds one Ph.D.-level graduate student (began in Jan 2013) and partial funding for one post-doctoral research associate, both of whom are focused on research with anaerobic soil disinfestation in lab, growth chamber, and field settings. Three community college students have been trained on the use of ASD and are working on senior thesis projects from these trials. One high school student has been engaged in an internship position looking at the impacts of ASD on microbial communities. How have the results been disseminated to communities of interest? Outreach during the reporting period included presentations at a roundtable organized by the California Strawberry Commission and creation of a website to highlight research projects, research publication, technical publications, and videos (available at http://vegetables.tennessee.edu/asd). Two hands-on demonstrations have been conducted in Florida in cooperation with the Saint Lucie County Extension Service. What do you plan to do during the next reporting period to accomplish the goals? For Obj. 1, analysis of data and preparation of manuscripts from pot and field studies will continue. For Obj. 2, field trials will continue on the evaluation of organisms of interest related to food safety concerns, including augmentation studies to be initiated in the spring. For Obj. 3, the current field trial will be completed and the trial will be repeated. For Obj. 4, outreach activities will continue through a variety of methods and venues, including technical and scientific publications, field days, on-farm studies, and scientific meetings (including MBAO).
Impacts What was accomplished under these goals?
For Obj. 1, the second year of growth chamber and field studies were completed in TN. Growth chamber studies (repeated) evaluated ASD C source and C:N ratio impacts on yellow nutsedge, Sclerotium rolfsii, and Fusarium oxysporum. The field study evaluated C:N ratio of ASD soil amendments on mortality of introduced pathogen inoculum, disease incidence, crop productivity, weed control, and soil properties following ASD treatment. Results have been presented at professional meetings and publications and a dissertation are in progress. For Obj. 2, experiments were established to test for Salmonella with enrichment before and after ASD treatment. For Obj. 3, one field trial, using a split design with tomato and cucumber crops, was completed to determine if the combination of organic acids with solarization and ASD would provide disease control. A second experiment was established to assess the ability to suppress plants diseases POST-treatment application. For Obj. 4, we have continued to present results of these trials at scientific meetings and field days (see following sections).
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2013
Citation:
Shrestha, U., B.H. Ownley, E.N. Rosskopf, M.E. Dee and D.M. Butler. 2013. Optimization of amendment C:N ratio in anaerobic soil disinfestation for control of Sclerotium rolfsii. Proceedings of the Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions; pp. 14-1 to 14-3.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Shrestha, U., A. Bruce, B.H. Ownley and D.M. Butler. 2014. Effects of organic amendment C:N ratio on Fusarium oxysporum f. sp. lycopersici populations following anaerobic soil disinfestation. Phytopathology 104:S3.108 (abstract).
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Hong, J.C., K.J. Martin, N. Kokalis-Burelle, D.M. Butler and E.N. Rosskopf. 2014. Comparison of soil bacterial communities in fields treated with anaerobic soil disinfestation located on Floridas east to west coast. Phytopathology 104:S3.52 (abstract).
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2014
Citation:
Rosskopf, E.N., D.M. Butler, N. Kokalis-Burelle and J.C. Hong. 2014. Using anaerobic soil disinfestation in organic vegetable production. Annual Conference of the American Society for Horticultural Science; Orlando, FL, 28-31 Jul 2014. NIFA support acknowledged? Yes
- Type:
Websites
Status:
Published
Year Published:
2014
Citation:
http://vegetables.tennessee.edu/asd
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Progress 09/01/12 to 08/31/13
Outputs Target Audience: Target audiences of scientific presentations at the American Phytopathology Meetings during the reporting period included plant pathologists, weed scientists, soil scientists, horticulturalists, agricultural professionals and consultants, extension specialists, and policy makers from the USA and other countries. Target audiences of extension and outreach presentations included farmers, extension personnel, and agricultural consultants. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? In addition to outreach activities to growers and agricultural professionals (detailed below), this project funds one Ph.D.-level graduate student (began in Jan 2013) and partial funding for one post-doctoral research associate, both of whom are focused on research with anaerobic soil disinfestation in lab, growth chamber, and field settings. How have the results been disseminated to communities of interest? In TN, outreach included a presentation to growers at the TN Horticulture Expo in January of 2013 and 3 presentations at a field day held at a university research site (Plateau Research and Education Center) in August of 2013. What do you plan to do during the next reporting period to accomplish the goals? For Obj. 1, analysis of data from pot and field studies will continue. The field study will be repeated during the 2014 field season. For Obj. 2, field trials will continue on the evaluation of organisms of interest related to food safety concerns, including augmentation studies to be initiated in the spring. For Obj. 3, the current field trial will be completed and the trial will be repeated. For Obj. 4, outreach activities will continue through a variety of methods and venues, including technical and scientific publications, field days, on-farm studies, and scientific meetings (including MBAO).
Impacts What was accomplished under these goals?
For Obj. 1, growth chamber and field studies were initiated in TN soon after the beginning of the project. Growth chamber studies (repeated) evaluated ASD C source and C:N ratio impacts on yellow nutsedge, Sclerotium rolfsii, and Fusarium oxysporum. A field study was initiated to evaluate C:N ratio of ASD soil amendments on mortality of introduced pathogen inoculum, disease incidence, crop productivity, weed control, and soil properties following ASD treatment. Data analysis is in progress and the first results will be presented at the MBAO conference in fall of 2013. For Obj. 2, two field trials were conducted to evaluate corn gluten meal, soybean meal, pasteurized broiler litter pellets, mustard seed meal, and chitin as replacements for partially composted broiler litter. All inputs, including litter, were tested for Salmonella and hemorrhagic Escherichia coli and none had detectable levels. For Obj. 3, one field trial, using a split design with tomato and cucumber crops, was established to determine if pest control could be improved by using a combination of ASD treatment, solarization, and organic acid application. For Obj. 4, we have already begun to present results of these trials at scientific meetings, grower meetings, and field days (see following sections).
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2013
Citation:
Hong, J.C., K.J. Martin, N. Kokalis-Burelle, D.M. Butler and E.N. Rosskopf. 2013. Bacterial population changes in fields treated with anaerobic soil disinfestation. Phytopathology 103(Suppl. 2):S2.61 (abstract).
- Type:
Conference Papers and Presentations
Status:
Submitted
Year Published:
2013
Citation:
Hong, J.C., K.J. Martin, N. Kokalis-Burelle, D.M. Butler and E.N. Rosskopf. 2013. Identifying bacterial populations potentially integral to anaerobic soil disinfestation. 5th Annual Argonne Soil Metagenomics Meeting Abstracts; Bloomingdale, IL, 2-4 Oct 2013. (submitted)
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