Progress 05/01/23 to 04/30/24
Outputs
Target Audience:Research community Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?One PhD student (Valerie Seitz) and one MS student (Arsen Yerlan) successfully completed their degree programs during the course of this project. They both received funded from this award and their theses are a direct outcome of this project. How have the results been disseminated to communities of interest?Schipanski, M. Soil health: Lessons learned from the higher and drier Central Great Plains. University of Nebraska Soil Health Meeting--Keynote address, Hastings, NE. January 29, 2025. What do you plan to do during the next reporting period to accomplish the goals?This project is complete.
Impacts
What was accomplished under these goals?
Objective 1. This objective has been completed and the results have been published. Objective 2: The results from Obj 1 informed the choice of 4 cover crop species from which exudates were collected and used to "feed" laboratory scale microcosms. This experiment has been completed and the results have been published. Objective 3. We completed the short-term field experiment evaluating four different cover crop species (Secale cereale, Vicia villosa, Brassica napus, and Sorghum bicolor) and a fallow control grown from August 2022 to May 2023. Maize was then planted in all plots and subsequently harvested in fall 2023. Soil samples collected at cover crop termination and peak maize biomass were analyzed for soil extracellular enzymes, organic carbon, nitrogen, soil aggregate stability, and microbial community metrics. Prior to cover crop termination, Secale cereale (rye) and Brassica napus increased aggregate stability and nitrogen-cycling enzyme activity, respectively, relative to other treatments. The cover crop treatment effects only persisted for rye into the following corn crop via increased carbon and phosphorus cycling enzyme activity. We identified key correlations between microbial community metrics and soil enzyme activity along with evidence of a legacy effect from certain cover crops. Our research shows that within a single season, cover crops can modify the soil microbiome at an organismal level and influence soil functions, demonstrating the potential scalability of findings from controlled studies to more complex field environments.The graduate student working on this project successfully defended his thesis and graduated. The publication will be submitted within the next 2 months.?
Publications
- Type:
Peer Reviewed Journal Articles
Status:
Accepted
Year Published:
2023
Citation:
14. Seitz, V. A.; Chaparro, J. M.; Schipanski, M. E.; Wrighton, K. C.; Prenni, J. E.* Cover Crop Cultivar, Species, and Functional Diversity is Reflected in Variable Root Exudation Composition. Journal of Agricultural and Food Chemistry 2023, 71 (30), 11373-11385. DOI: 10.1021/acs.jafc.3c02912.
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Progress 05/01/22 to 04/30/23
Outputs
Target Audience:Research community Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The Ph.D. student that was leading Obj. 1 & 2 sucessfully defended her thesis in august of 2023. During this project period she engaged in the following professional development activities: Vice President for Research Professional Development Workshop Series, Colorado State UniversitySchool of Global Environmental Sustainability Leadership Professional Development and Science Communication Workshop Series, Colorado State University. During this project she has also become proficient in both amplicon and metagenomic sequencing (sample preparation/library building and data analysis) as well as nontargete metabolite analysis and metaprotomics. The M.S. student researcher (involved in Obj. 3)has received extensive training in field and lab methods, project management and teamwork. The student has presented his research proposal to the CSU Agroecology Research Group. Also, in Spring 2023, the student researcher took an in-person data science class offered at CSU to improve R coding and data visualization skills and then led an R coding training for peers. How have the results been disseminated to communities of interest?Oral Presentations Valerie Seitz: "Cover Crops, the Soil Microbiome, and Soil Health - How Plants Feed their Friends", Guest seminar, University of North Carolina Chapel Hill. Virtual Valerie Seitz: "Cover Crops, the Soil Microbiome, and Soil Health - How Plants Feed their Friends", Cell & Molecular Biology program seminar, Colorado State University, Fort Collins, CO. Poster Presentations Valerie A Seitz, Jacqueline Chaparro, Meagan Schipanski, Kelly Wrighton, Nancy Jo Ehlke, Nicholas Wiering, Jessica Prenni, "Cover crop cultivar, species, and functional diversity is reflected in variable root exudation composition". Annual Conference for the American Society for Mass Spectrometry. Houston, TX. Valerie A Seitz, Jacqueline Chaparro, Meagan Schipanski, Kelly Wrighton, Nancy Jo Ehlke, Nicholas Wiering, Jessica Prenni, "Characterizing root exudate composition across 19 cover crop species". Graduate Student Showcase. Colorado State University, Fort Collins, CO. What do you plan to do during the next reporting period to accomplish the goals?Obj. 2: Complete data interpretation and manuscript preparation, submit manuscript. Obj. 3:In the coming year, we will complete all lab analyses from the field experiment, conduct statistical tests and analyses, present and publish results. We also initiated a second cycle of the experiment that will run into 2025 in an adjacent field.
Impacts
What was accomplished under these goals?
Objective 1. This objective has been completed and the results have been published. Objective 2:The results from Obj 1 informed the choice of 4 cover crop species from which exudates were collected and used to "feed" laboratory scale microcosms. This experiment has been completed and the resulting samples were anlyzed by metabolomics, metaproteomics, metatrascriptomics, and metagenomics. The manuscript describing the results of this experiment is in preparation and will be submitted in the fall of 2023. Objective 3. The same 4 cover crops selected for Obj. 2 were planted in the field in fall 2022. Specifically, we established a field experiment fall of 2022 with 5 replicate blocks and 4 cover crop treatments (hairy vetch, cereal rye, sorghum, and rape seed) along with a control plot. Cover crops were terminated in early May 2023 and corn was planted. We collected soil and plant samples before cover crop termination and again corn growth (July). To link the field experiment to the root exudate and microbiome lab analyses, we separated rhizosphere and bulk soil to analyze for cover crop effects on inorganic nitrogen, soil microbial communities and dissolved organic carbon content. We are also analyzing soil aggregation as a key soil health indicator.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2023
Citation:
Seitz, V. A.; Chaparro, J. M.; Schipanski, M. E.; Wrighton, K. C.; Prenni, J. E. Cover Crop Cultivar, Species, and Functional Diversity is Reflected in Variable Root Exudation Composition. Journal of Agricultural and Food Chemistry 2023, 71 (30), 11373-11385. DOI: 10.1021/acs.jafc.3c02912.
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Progress 05/01/21 to 04/30/22
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The Ph.D. student involved in this project has engaged in multiple training and professional development opportunities including: Rigor & Reproducibility Workshop, Center for Open Science, 2022 PNNL Environmental Molecular Science Laboratory Metabolic Modeling Workshop, chosen applicant to participate in data analysis workshops, 2021 3rd Annual NCQBCS Mass Spectrometry Summer School, 3-day workshop, 2021 How have the results been disseminated to communities of interest?The results have been disseminated in a number of poster and oral presentation: 1.) Jessica Prenni (Invited Speaker) "Cover Crops and the Soil Microbiome - How plants feed their friends", Online workshop, Investigating Plant Associated Microbiomes with Multiple -Omics Techniques, The Ohio State University, virtual. 2.) Valerie A Seitz "How cover crops can shape the composition and function of the soil microbiome". Vice President for Research (VPR) Graduate Student Fellowship 3-Minute Challenge. (Talk). Office of the Vice President for Research. 2022-2023 fellow. 3.) Valerie A Seitz, Jacqueline Chaparro, Bridget McGivern, Mikayla Borton, Reb Daly, Kelly Wrighton, Meagan Schipanski, Jessica Prenni. "Genotypic variation root exudate composition drives specific soil microbial membership and composition". Poster Presentation. Fall Graduate Student Showcase. Virtual (poster). Student Choice Award. 4.) Valerie A Seitz, Jacqueline Chaparro, Bridget McGivern, Mikayla Borton, Reb Daly, Kelly Wrighton, Meagan Schipanski, Jessica Prenni. "Genotypic variation root exudate composition drives specific soil microbial membership and composition". Poster Presentation. American Society for Mass Spectrometry. Virtual (poster). 5.) Valerie A Lindstrom, Jacqueline Chaparro, Bridget McGivern, Mikayla Borton, Reb Daly, Kelly Wrighton, Meagan Schipanski, Jessica Prenni. "Genotypic variation root exudate composition drives specific soil microbial assemblage". Student Flash Talk. NCQBCS Mass Spectrometry Summer School. Virtual (talk). 6.) Valerie A Lindstrom, Jacqueline Chaparro, Bridget McGivern, Mikayla Borton, Reb Daly, Kelly Wrighton, Meagan Schipanski, Jessica Prenni "Cover crops, the soil microbiome, and soil health - how plants feed their friends". Spring Cell and Molecular Biology Seminar, Colorado State University. Virtual. (talk) What do you plan to do during the next reporting period to accomplish the goals?Objective 1: Complete analysis of exudate profiles, compile results and submit for publication. Objective 2: Complete microcosm experiment with cover crop derived exudates. Submit samples for metagenomics, 16s rRNA, and metatranscriptomics analysis. Perform metaproteomics and exometabolomics analysis. Objective 3: Plant 4 cover crop species in field and collect soil samples as well as phenotypic data.
Impacts
What was accomplished under these goals?
Objective 1: Exudates have been generated for 18 species of cover crops. The exudates have been analyzed by GC-MS and LC-MS and data analysis is underway. The results of this analysis will be compiled for publication by the end of 2022. Objective 2: The results from Obj 1 have informed the choice of 4 cover crop species from which exudates will be collected and used to "feed" laboratory scale microcosms. These exudates are currently being produced and text microcosm experiments are underway. These experiments will be performed in summer 2022. Objective 3: The same 4 cover crop species will be planted in the field in Fall of 2022. The field has been identified and reserved. A Masters student has been recruited to lead the field experiments and will be starting also Fall of 2022.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2022
Citation:
Valerie A Seitz, Bridget B McGivern, Rebecca A Daly, Jacqueline M Chaparro Mikayla A Borton, Amy M Sheflin, Stephen Kresovich, Lindsay Shields, Meagan E Schipanski, Kelly C Wrighton and Jessica E Prenni Variation in root exudate composition influences soil microbiome membership and function , Applied and Environmental Microbiology, 2022 (Accepted 4/14/22)
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